Details of the Target

General Information of Target

Target ID LDTP06702
Target Name WASH complex subunit 4 (WASHC4)
Gene Name WASHC4
Gene ID 23325
Synonyms
KIAA1033; WASH complex subunit 4; Strumpellin and WASH-interacting protein; SWIP; WASH complex subunit SWIP
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MAVETLSPDWEFDRVDDGSQKIHAEVQLKNYGKFLEEYTSQLRRIEDALDDSIGDVWDFN
LDPIALKLLPYEQSSLLELIKTENKVLNKVITVYAALCCEIKKLKYEAETKFYNGLLFYG
EGATDASMVEGDCQIQMGRFISFLQELSCFVTRCYEVVMNVVHQLAALYISNKIAPKIIE
TTGVHFQTMYEHLGELLTVLLTLDEIIDNHITLKDHWTMYKRLLKSVHHNPSKFGIQEEK
LKPFEKFLLKLEGQLLDGMIFQACIEQQFDSLNGGVSVSKNSTFAEEFAHSIRSIFANVE
AKLGEPSEIDQRDKYVGICGLFVLHFQIFRTIDKKFYKSLLDICKKVPAITLTANIIWFP
DNFLIQKIPAAAKLLDRKSLQAIKIHRDTFLQQKAQSLTKDVQSYYVFVSSWMMKMESIL
SKEQRMDKFAEDLTNRCNVFIQGFLYAYSISTIIKTTMNLYMSMQKPMTKTSVKALCRLV
ELLKAIEHMFYRRSMVVADSVSHITQHLQHQALHSISVAKKRVISDKKYSEQRLDVLSAL
VLAENTLNGPSTKQRRLIVSLALSVGTQMKTFKDEELFPLQVVMKKLDLISELRERVQTQ
CDCCFLYWHRAVFPIYLDDVYENAVDAARLHYMFSALRDCVPAMMHARHLESYEILLDCY
DKEIMEILNEHLLDKLCKEIEKDLRLSVHTHLKLDDRNPFKVGMKDLALFFSLNPIRFFN
RFIDIRAYVTHYLDKTFYNLTTVALHDWATYSEMRNLATQRYGLVMTEAHLPSQTLEQGL
DVLEIMRNIHIFVSRYLYNLNNQIFIERTSNNKHLNTINIRHIANSIRTHGTGIMNTTVN
FTYQFLKKKFYIFSQFMYDEHIKSRLIKDIRFFREIKDQNDHKYPFDRAEKFNRGIRKLG
VTPEGQSYLDQFRQLISQIGNAMGYVRMIRSGGLHCSSNAIRFVPDLEDIVNFEELVKEE
GLAEETLKAARHLDSVLSDHTRNSAEGTEYFKMLVDVFAPEFRRPKNIHLRNFYIIVPPL
TLNFVEHSISCKEKLNKKNKIGAAFTDDGFAMGVAYILKLLDQYREFDSLHWFQSVREKY
LKEIRAVAKQQNVQSASQDEKLLQTMNLTQKRLDVYLQEFELLYFSLSSARIFFRADKTA
AEENQEKKEKEEETKTSNGDLSDSTVSADPVVK
Target Bioclass
Transporter and channel
Family
SWIP family
Subcellular location
Early endosome
Function
Acts as a component of the WASH core complex that functions as a nucleation-promoting factor (NPF) at the surface of endosomes, where it recruits and activates the Arp2/3 complex to induce actin polymerization, playing a key role in the fission of tubules that serve as transport intermediates during endosome sorting.
Uniprot ID
Q2M389
Ensemble ID
ENST00000332180.10
HGNC ID
HGNC:29174

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
HCT15 SNV: p.N1158D .
HEL SNV: p.E4D DBIA    Probe Info 
HEL9217 SNV: p.E4D DBIA    Probe Info 
HEPG2 SNV: p.L241I .
HT115 SNV: p.K29N .
HUH28 SNV: p.L947V .
KYM1 Deletion: p.P550_S551del .
KYSE30 SNV: p.P699L .
LS180 Deletion: p.S379VfsTer31 .
NCIH1299 SNV: p.Q1090P .
PANC1 Substitution: p.V901I .
RL Substitution: p.V901I DBIA    Probe Info 
RL952 Deletion: p.N1039IfsTer20
Substitution: p.V901I		 DBIA    Probe Info 
SNU449 SNV: p.Q1104K .
SUPT1 SNV: p.R596Ter .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 19 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C-Sul
 Probe Info  		4.33  LDD0066  [1]
BTD
 Probe Info  		C936(2.21)  LDD1700  [2]
HHS-475
 Probe Info  		Y71(0.87)  LDD0264  [3]
Acrolein
 Probe Info  		N.A.  LDD0221  [4]
DBIA
 Probe Info  		C604(1.04)  LDD0080  [5]
N1
 Probe Info  		Q1104(0.00); Q1110(0.00)  LDD0245  [6]
4-Iodoacetamidophenylacetylene
 Probe Info  		C659(0.00); C1031(0.00); C344(0.00); C936(0.00)  LDD0038  [7]
IA-alkyne
 Probe Info  		C640(0.00); C1031(0.00); C936(0.00); C344(0.00)  LDD0036  [7]
Lodoacetamide azide
 Probe Info  		C640(0.00); C1031(0.00); C344(0.00); C149(0.00)  LDD0037  [7]
ATP probe
 Probe Info  		N.A.  LDD0035  [8]
NAIA_4
 Probe Info  		N.A.  LDD2226  [9]
IPM
 Probe Info  		N.A.  LDD0147  [10]
NHS
 Probe Info  		K302(0.00); K586(0.00)  LDD0010  [11]
STPyne
 Probe Info  		N.A.  LDD0009  [11]
TFBX
 Probe Info  		C344(0.00); C936(0.00)  LDD0148  [10]
Methacrolein
 Probe Info  		N.A.  LDD0218  [4]
W1
 Probe Info  		D887(0.00); Y884(0.00)  LDD0236  [12]
NAIA_5
 Probe Info  		C936(0.00); C344(0.00)  LDD2223  [9]
HHS-482
 Probe Info  		Y71(1.62)  LDD2239  [13]
PAL-AfBPP Probe
Click To Hide/Show 3 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C314
 Probe Info  		30.27  LDD1981  [14]
FFF probe13
 Probe Info  		9.57  LDD0475  [15]
FFF probe3
 Probe Info  		20.00  LDD0465  [15]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0524  2-Cyano-N-(2-morpholin-4-yl-ethyl)-acetamide MDA-MB-231 C936(0.96)  LDD2117  [2]
 LDCM0214  AC1 HCT 116 C344(1.52)  LDD0531  [5]
 LDCM0215  AC10 HEK-293T C601(1.34); C603(1.34); C604(1.34)  LDD0813  [5]
 LDCM0216  AC100 HCT 116 C344(0.64); C604(1.25)  LDD0533  [5]
 LDCM0217  AC101 HCT 116 C344(0.67); C604(1.18)  LDD0534  [5]
 LDCM0218  AC102 HCT 116 C344(0.68); C604(1.02)  LDD0535  [5]
 LDCM0219  AC103 HCT 116 C344(0.63); C604(1.07)  LDD0536  [5]
 LDCM0220  AC104 HCT 116 C344(0.65); C604(1.12)  LDD0537  [5]
 LDCM0221  AC105 HCT 116 C344(0.65); C604(1.28)  LDD0538  [5]
 LDCM0222  AC106 HCT 116 C344(0.65); C604(1.15)  LDD0539  [5]
 LDCM0223  AC107 HCT 116 C344(0.54); C604(1.23)  LDD0540  [5]
 LDCM0224  AC108 HCT 116 C344(0.53); C604(1.46)  LDD0541  [5]
 LDCM0225  AC109 HCT 116 C344(0.56); C604(0.91)  LDD0542  [5]
 LDCM0226  AC11 HEK-293T C601(1.21); C603(1.21); C604(1.21)  LDD0824  [5]
 LDCM0227  AC110 HCT 116 C344(0.52); C604(1.23)  LDD0544  [5]
 LDCM0228  AC111 HCT 116 C344(0.52); C604(1.03)  LDD0545  [5]
 LDCM0229  AC112 HCT 116 C344(0.53); C604(0.96)  LDD0546  [5]
 LDCM0230  AC113 HEK-293T C604(0.88)  LDD0828  [5]
 LDCM0231  AC114 HEK-293T C604(0.94)  LDD0829  [5]
 LDCM0232  AC115 HEK-293T C604(0.87)  LDD0830  [5]
 LDCM0233  AC116 HEK-293T C604(0.85)  LDD0831  [5]
 LDCM0234  AC117 HEK-293T C604(0.82)  LDD0832  [5]
 LDCM0235  AC118 HEK-293T C604(0.97)  LDD0833  [5]
 LDCM0236  AC119 HEK-293T C604(0.72)  LDD0834  [5]
 LDCM0237  AC12 HEK-293T C601(0.91); C603(0.91); C604(0.91)  LDD0835  [5]
 LDCM0238  AC120 HEK-293T C604(0.84)  LDD0836  [5]
 LDCM0239  AC121 HEK-293T C604(0.81)  LDD0837  [5]
 LDCM0240  AC122 HEK-293T C604(0.65)  LDD0838  [5]
 LDCM0241  AC123 HEK-293T C604(0.64)  LDD0839  [5]
 LDCM0242  AC124 HEK-293T C604(0.77)  LDD0840  [5]
 LDCM0243  AC125 HEK-293T C604(0.64)  LDD0841  [5]
 LDCM0244  AC126 HEK-293T C604(0.51)  LDD0842  [5]
 LDCM0245  AC127 HEK-293T C604(0.58)  LDD0843  [5]
 LDCM0246  AC128 HEK-293T C601(1.09); C603(1.13); C604(1.13)  LDD0844  [5]
 LDCM0247  AC129 HEK-293T C601(0.85); C603(0.80); C604(0.80)  LDD0845  [5]
 LDCM0249  AC130 HEK-293T C601(0.94); C603(1.02); C604(1.02)  LDD0847  [5]
 LDCM0250  AC131 HEK-293T C601(0.92); C603(0.94); C604(0.94)  LDD0848  [5]
 LDCM0251  AC132 HEK-293T C601(0.99); C603(1.10); C604(1.10)  LDD0849  [5]
 LDCM0252  AC133 HEK-293T C601(0.88); C603(0.79); C604(0.79)  LDD0850  [5]
 LDCM0253  AC134 HEK-293T C601(0.98); C603(1.01); C604(1.01)  LDD0851  [5]
 LDCM0254  AC135 HEK-293T C601(0.93); C603(0.93); C604(0.93)  LDD0852  [5]
 LDCM0255  AC136 HEK-293T C601(0.94); C603(0.84); C604(0.84)  LDD0853  [5]
 LDCM0256  AC137 HEK-293T C601(0.93); C603(0.88); C604(0.88)  LDD0854  [5]
 LDCM0257  AC138 HEK-293T C601(1.01); C603(0.99); C604(0.99)  LDD0855  [5]
 LDCM0258  AC139 HEK-293T C601(1.04); C603(1.02); C604(1.02)  LDD0856  [5]
 LDCM0259  AC14 HEK-293T C601(0.70); C603(0.70); C604(0.70)  LDD0857  [5]
 LDCM0260  AC140 HEK-293T C601(0.95); C603(0.91); C604(0.91)  LDD0858  [5]
 LDCM0261  AC141 HEK-293T C601(1.08); C603(1.16); C604(1.16)  LDD0859  [5]
 LDCM0262  AC142 HEK-293T C601(0.97); C603(0.91); C604(0.91)  LDD0860  [5]
 LDCM0263  AC143 HEK-293T C601(1.07); C604(0.81)  LDD0861  [5]
 LDCM0264  AC144 HEK-293T C601(1.28); C604(0.52)  LDD0862  [5]
 LDCM0265  AC145 HEK-293T C601(1.27); C604(0.45)  LDD0863  [5]
 LDCM0266  AC146 HEK-293T C601(1.26); C604(0.50)  LDD0864  [5]
 LDCM0267  AC147 HEK-293T C601(1.31); C604(0.52)  LDD0865  [5]
 LDCM0268  AC148 HEK-293T C601(1.29); C604(0.61)  LDD0866  [5]
 LDCM0269  AC149 HEK-293T C601(1.22); C604(0.93)  LDD0867  [5]
 LDCM0270  AC15 HEK-293T C601(1.43); C603(1.43); C604(1.43)  LDD0868  [5]
 LDCM0271  AC150 HEK-293T C601(1.33); C604(0.76)  LDD0869  [5]
 LDCM0272  AC151 HEK-293T C601(1.23); C604(0.68)  LDD0870  [5]
 LDCM0273  AC152 HEK-293T C601(1.17); C604(0.68)  LDD0871  [5]
 LDCM0274  AC153 HEK-293T C601(1.16); C604(0.80)  LDD0872  [5]
 LDCM0621  AC154 HEK-293T C601(1.13); C604(0.80)  LDD2162  [5]
 LDCM0622  AC155 HEK-293T C601(1.23); C604(0.67)  LDD2163  [5]
 LDCM0623  AC156 HEK-293T C601(1.27); C604(0.50)  LDD2164  [5]
 LDCM0624  AC157 HEK-293T C601(1.39); C604(0.56)  LDD2165  [5]
 LDCM0276  AC17 HCT 116 C604(0.94)  LDD0593  [5]
 LDCM0277  AC18 HCT 116 C604(0.85)  LDD0594  [5]
 LDCM0278  AC19 HCT 116 C604(0.86)  LDD0595  [5]
 LDCM0279  AC2 HCT 116 C344(1.03)  LDD0596  [5]
 LDCM0280  AC20 HCT 116 C604(0.97)  LDD0597  [5]
 LDCM0281  AC21 HCT 116 C604(0.82)  LDD0598  [5]
 LDCM0282  AC22 HCT 116 C604(0.80)  LDD0599  [5]
 LDCM0283  AC23 HCT 116 C604(0.93)  LDD0600  [5]
 LDCM0284  AC24 HCT 116 C604(1.28)  LDD0601  [5]
 LDCM0285  AC25 PaTu 8988t C344(1.45); C603(0.93); C604(0.93)  LDD1164  [5]
 LDCM0286  AC26 PaTu 8988t C344(1.22); C603(0.61); C604(0.61)  LDD1165  [5]
 LDCM0287  AC27 PaTu 8988t C344(1.15); C603(0.64); C604(0.64)  LDD1166  [5]
 LDCM0288  AC28 PaTu 8988t C344(1.16); C603(1.10); C604(1.10)  LDD1167  [5]
 LDCM0289  AC29 PaTu 8988t C344(1.21); C603(0.70); C604(0.70)  LDD1168  [5]
 LDCM0290  AC3 HCT 116 C344(1.23)  LDD0607  [5]
 LDCM0291  AC30 PaTu 8988t C344(1.21); C603(0.92); C604(0.92)  LDD1170  [5]
 LDCM0292  AC31 PaTu 8988t C344(1.39); C603(0.98); C604(0.98)  LDD1171  [5]
 LDCM0293  AC32 PaTu 8988t C344(1.00); C603(1.47); C604(1.47)  LDD1172  [5]
 LDCM0294  AC33 PaTu 8988t C344(1.07); C603(1.06); C604(1.06)  LDD1173  [5]
 LDCM0295  AC34 PaTu 8988t C344(1.08); C603(1.25); C604(1.25)  LDD1174  [5]
 LDCM0296  AC35 HCT 116 C344(1.07)  LDD0613  [5]
 LDCM0297  AC36 HCT 116 C344(0.86)  LDD0614  [5]
 LDCM0298  AC37 HCT 116 C344(0.89)  LDD0615  [5]
 LDCM0299  AC38 HCT 116 C344(1.00)  LDD0616  [5]
 LDCM0300  AC39 HCT 116 C344(1.09)  LDD0617  [5]
 LDCM0301  AC4 HCT 116 C344(1.52)  LDD0618  [5]
 LDCM0302  AC40 HCT 116 C344(0.88)  LDD0619  [5]
 LDCM0303  AC41 HCT 116 C344(0.66)  LDD0620  [5]
 LDCM0304  AC42 HCT 116 C344(0.75)  LDD0621  [5]
 LDCM0305  AC43 HCT 116 C344(0.64)  LDD0622  [5]
 LDCM0306  AC44 HCT 116 C344(0.84)  LDD0623  [5]
 LDCM0307  AC45 HCT 116 C344(0.75)  LDD0624  [5]
 LDCM0308  AC46 HEK-293T C604(1.15)  LDD0906  [5]
 LDCM0309  AC47 HEK-293T C604(0.96)  LDD0907  [5]
 LDCM0310  AC48 HEK-293T C604(1.07)  LDD0908  [5]
 LDCM0311  AC49 HEK-293T C604(0.96)  LDD0909  [5]
 LDCM0312  AC5 HCT 116 C344(1.52)  LDD0629  [5]
 LDCM0313  AC50 HEK-293T C604(1.17)  LDD0911  [5]
 LDCM0314  AC51 HEK-293T C604(1.16)  LDD0912  [5]
 LDCM0315  AC52 HEK-293T C604(1.13)  LDD0913  [5]
 LDCM0316  AC53 HEK-293T C604(1.11)  LDD0914  [5]
 LDCM0317  AC54 HEK-293T C604(1.11)  LDD0915  [5]
 LDCM0318  AC55 HEK-293T C604(1.23)  LDD0916  [5]
 LDCM0319  AC56 HEK-293T C604(0.85)  LDD0917  [5]
 LDCM0320  AC57 HCT 116 C604(0.96)  LDD0637  [5]
 LDCM0321  AC58 HCT 116 C604(1.18)  LDD0638  [5]
 LDCM0322  AC59 HCT 116 C604(0.90)  LDD0639  [5]
 LDCM0323  AC6 HEK-293T C601(1.23); C603(1.23); C604(1.23)  LDD0921  [5]
 LDCM0324  AC60 HCT 116 C604(0.86)  LDD0641  [5]
 LDCM0325  AC61 HCT 116 C604(0.87)  LDD0642  [5]
 LDCM0326  AC62 HCT 116 C604(0.82)  LDD0643  [5]
 LDCM0327  AC63 HCT 116 C604(0.77)  LDD0644  [5]
 LDCM0328  AC64 HCT 116 C604(0.87)  LDD0645  [5]
 LDCM0329  AC65 HCT 116 C604(1.11)  LDD0646  [5]
 LDCM0330  AC66 HCT 116 C604(1.07)  LDD0647  [5]
 LDCM0331  AC67 HCT 116 C604(1.18)  LDD0648  [5]
 LDCM0332  AC68 HCT 116 C604(0.79)  LDD0649  [5]
 LDCM0333  AC69 HCT 116 C604(0.77)  LDD0650  [5]
 LDCM0334  AC7 HEK-293T C601(1.19); C603(1.19); C604(1.19)  LDD0932  [5]
 LDCM0335  AC70 HCT 116 C604(0.99)  LDD0652  [5]
 LDCM0336  AC71 HCT 116 C604(0.63)  LDD0653  [5]
 LDCM0337  AC72 HCT 116 C604(0.85)  LDD0654  [5]
 LDCM0338  AC73 HCT 116 C604(0.83)  LDD0655  [5]
 LDCM0339  AC74 HCT 116 C604(1.12)  LDD0656  [5]
 LDCM0340  AC75 HCT 116 C604(0.91)  LDD0657  [5]
 LDCM0341  AC76 HCT 116 C604(0.65)  LDD0658  [5]
 LDCM0342  AC77 HCT 116 C604(1.01)  LDD0659  [5]
 LDCM0343  AC78 HCT 116 C604(0.73)  LDD0660  [5]
 LDCM0344  AC79 HCT 116 C604(1.19)  LDD0661  [5]
 LDCM0345  AC8 HEK-293T C601(0.81); C603(0.81); C604(0.81)  LDD0943  [5]
 LDCM0346  AC80 HCT 116 C604(0.67)  LDD0663  [5]
 LDCM0347  AC81 HCT 116 C604(0.77)  LDD0664  [5]
 LDCM0348  AC82 HCT 116 C604(0.89)  LDD0665  [5]
 LDCM0349  AC83 HCT 116 C604(1.40)  LDD0666  [5]
 LDCM0350  AC84 HCT 116 C604(1.36)  LDD0667  [5]
 LDCM0351  AC85 HCT 116 C604(1.19)  LDD0668  [5]
 LDCM0352  AC86 HCT 116 C604(1.25)  LDD0669  [5]
 LDCM0353  AC87 HCT 116 C604(1.32)  LDD0670  [5]
 LDCM0354  AC88 HCT 116 C604(1.95)  LDD0671  [5]
 LDCM0355  AC89 HCT 116 C604(1.86)  LDD0672  [5]
 LDCM0357  AC90 HCT 116 C604(1.29)  LDD0674  [5]
 LDCM0358  AC91 HCT 116 C604(1.65)  LDD0675  [5]
 LDCM0359  AC92 HCT 116 C604(2.23)  LDD0676  [5]
 LDCM0360  AC93 HCT 116 C604(1.95)  LDD0677  [5]
 LDCM0361  AC94 HCT 116 C604(1.49)  LDD0678  [5]
 LDCM0362  AC95 HCT 116 C604(0.94)  LDD0679  [5]
 LDCM0363  AC96 HCT 116 C604(1.97)  LDD0680  [5]
 LDCM0364  AC97 HCT 116 C604(1.55)  LDD0681  [5]
 LDCM0365  AC98 HCT 116 C344(0.47); C604(1.36)  LDD0682  [5]
 LDCM0366  AC99 HCT 116 C344(0.83); C604(1.20)  LDD0683  [5]
 LDCM0545  Acetamide MDA-MB-231 C936(0.38)  LDD2138  [2]
 LDCM0248  AKOS034007472 HEK-293T C601(0.95); C603(0.95); C604(0.95)  LDD0846  [5]
 LDCM0356  AKOS034007680 HEK-293T C601(1.05); C603(1.05); C604(1.05)  LDD0954  [5]
 LDCM0275  AKOS034007705 HEK-293T C601(1.16); C603(1.16); C604(1.16)  LDD0873  [5]
 LDCM0020  ARS-1620 HCC44 C604(1.04)  LDD2171  [5]
 LDCM0108  Chloroacetamide HeLa C344(0.00); H972(0.00)  LDD0222  [4]
 LDCM0367  CL1 HCT 116 C344(1.04)  LDD0684  [5]
 LDCM0368  CL10 HCT 116 C344(1.14)  LDD0685  [5]
 LDCM0369  CL100 HCT 116 C344(1.42)  LDD0686  [5]
 LDCM0370  CL101 HEK-293T C601(1.23); C603(1.23); C604(1.23)  LDD0968  [5]
 LDCM0371  CL102 HEK-293T C601(1.04); C603(1.04); C604(1.04)  LDD0969  [5]
 LDCM0372  CL103 HEK-293T C601(0.92); C603(0.92); C604(0.92)  LDD0970  [5]
 LDCM0373  CL104 HEK-293T C601(1.12); C603(1.12); C604(1.12)  LDD0971  [5]
 LDCM0374  CL105 HCT 116 C604(0.74)  LDD0691  [5]
 LDCM0375  CL106 HCT 116 C604(0.68)  LDD0692  [5]
 LDCM0376  CL107 HCT 116 C604(0.76)  LDD0693  [5]
 LDCM0377  CL108 HCT 116 C604(0.75)  LDD0694  [5]
 LDCM0378  CL109 HCT 116 C604(0.86)  LDD0695  [5]
 LDCM0379  CL11 HCT 116 C344(1.19)  LDD0696  [5]
 LDCM0380  CL110 HCT 116 C604(0.93)  LDD0697  [5]
 LDCM0381  CL111 HCT 116 C604(0.94)  LDD0698  [5]
 LDCM0382  CL112 PaTu 8988t C344(0.97); C603(1.15); C604(1.15)  LDD1261  [5]
 LDCM0383  CL113 PaTu 8988t C344(1.34); C603(0.81); C604(0.81)  LDD1262  [5]
 LDCM0384  CL114 PaTu 8988t C344(1.24); C603(0.98); C604(0.98)  LDD1263  [5]
 LDCM0385  CL115 PaTu 8988t C344(1.30); C603(0.76); C604(0.76)  LDD1264  [5]
 LDCM0386  CL116 PaTu 8988t C344(0.79); C603(0.83); C604(0.83)  LDD1265  [5]
 LDCM0387  CL117 HCT 116 C344(1.06)  LDD0704  [5]
 LDCM0388  CL118 HCT 116 C344(0.77)  LDD0705  [5]
 LDCM0389  CL119 HCT 116 C344(0.75)  LDD0706  [5]
 LDCM0390  CL12 HCT 116 C344(0.99)  LDD0707  [5]
 LDCM0391  CL120 HCT 116 C344(0.74)  LDD0708  [5]
 LDCM0392  CL121 HEK-293T C604(1.00)  LDD0990  [5]
 LDCM0393  CL122 HEK-293T C604(1.16)  LDD0991  [5]
 LDCM0394  CL123 HEK-293T C604(0.93)  LDD0992  [5]
 LDCM0395  CL124 HEK-293T C604(0.99)  LDD0993  [5]
 LDCM0396  CL125 HCT 116 C604(0.96)  LDD0713  [5]
 LDCM0397  CL126 HCT 116 C604(0.95)  LDD0714  [5]
 LDCM0398  CL127 HCT 116 C604(1.02)  LDD0715  [5]
 LDCM0399  CL128 HCT 116 C604(1.06)  LDD0716  [5]
 LDCM0400  CL13 HCT 116 C344(0.97)  LDD0717  [5]
 LDCM0401  CL14 HCT 116 C344(1.12)  LDD0718  [5]
 LDCM0402  CL15 HCT 116 C344(1.27)  LDD0719  [5]
 LDCM0403  CL16 PaTu 8988t C601(0.83); C603(0.83); C149(1.23); C604(0.83)  LDD1282  [5]
 LDCM0404  CL17 HCT 116 C344(0.93)  LDD0721  [5]
 LDCM0405  CL18 HCT 116 C344(0.92)  LDD0722  [5]
 LDCM0406  CL19 HCT 116 C344(0.97)  LDD0723  [5]
 LDCM0407  CL2 HCT 116 C344(1.01)  LDD0724  [5]
 LDCM0408  CL20 HCT 116 C344(0.92)  LDD0725  [5]
 LDCM0409  CL21 HCT 116 C344(0.88)  LDD0726  [5]
 LDCM0410  CL22 HCT 116 C344(0.88)  LDD0727  [5]
 LDCM0411  CL23 HCT 116 C344(1.02)  LDD0728  [5]
 LDCM0412  CL24 HCT 116 C344(1.03)  LDD0729  [5]
 LDCM0413  CL25 HCT 116 C344(1.01)  LDD0730  [5]
 LDCM0414  CL26 HCT 116 C344(0.99)  LDD0731  [5]
 LDCM0415  CL27 HCT 116 C344(1.09)  LDD0732  [5]
 LDCM0416  CL28 HCT 116 C344(1.13)  LDD0733  [5]
 LDCM0417  CL29 HCT 116 C344(0.77)  LDD0734  [5]
 LDCM0418  CL3 HCT 116 C344(1.32)  LDD0735  [5]
 LDCM0419  CL30 HCT 116 C344(1.05)  LDD0736  [5]
 LDCM0420  CL31 HCT 116 C344(1.14)  LDD0737  [5]
 LDCM0421  CL32 HEK-293T C601(1.26); C604(1.41)  LDD1019  [5]
 LDCM0422  CL33 HEK-293T C601(0.99); C604(1.10)  LDD1020  [5]
 LDCM0423  CL34 HEK-293T C601(0.89); C604(1.15)  LDD1021  [5]
 LDCM0424  CL35 HEK-293T C601(1.41); C604(1.15)  LDD1022  [5]
 LDCM0425  CL36 HEK-293T C601(1.32); C604(1.45)  LDD1023  [5]
 LDCM0426  CL37 HEK-293T C601(0.82); C604(1.03)  LDD1024  [5]
 LDCM0428  CL39 HEK-293T C601(0.87); C604(1.12)  LDD1026  [5]
 LDCM0429  CL4 HCT 116 C344(0.92)  LDD0746  [5]
 LDCM0430  CL40 HEK-293T C601(0.96); C604(1.43)  LDD1028  [5]
 LDCM0431  CL41 HEK-293T C601(1.25); C604(1.06)  LDD1029  [5]
 LDCM0432  CL42 HEK-293T C601(1.24); C604(1.34)  LDD1030  [5]
 LDCM0433  CL43 HEK-293T C601(1.05); C604(1.14)  LDD1031  [5]
 LDCM0434  CL44 HEK-293T C601(1.49); C604(1.18)  LDD1032  [5]
 LDCM0435  CL45 HEK-293T C601(1.08); C604(0.96)  LDD1033  [5]
 LDCM0436  CL46 HCT 116 C344(0.83)  LDD0753  [5]
 LDCM0437  CL47 HCT 116 C344(0.78)  LDD0754  [5]
 LDCM0438  CL48 HCT 116 C344(0.88)  LDD0755  [5]
 LDCM0439  CL49 HCT 116 C344(1.10)  LDD0756  [5]
 LDCM0440  CL5 HCT 116 C344(1.20)  LDD0757  [5]
 LDCM0441  CL50 HCT 116 C344(1.08)  LDD0758  [5]
 LDCM0442  CL51 HCT 116 C344(0.96)  LDD0759  [5]
 LDCM0443  CL52 HCT 116 C344(0.85)  LDD0760  [5]
 LDCM0444  CL53 HCT 116 C344(0.74)  LDD0761  [5]
 LDCM0445  CL54 HCT 116 C344(0.94)  LDD0762  [5]
 LDCM0446  CL55 HCT 116 C344(1.06)  LDD0763  [5]
 LDCM0447  CL56 HCT 116 C344(0.85)  LDD0764  [5]
 LDCM0448  CL57 HCT 116 C344(0.85)  LDD0765  [5]
 LDCM0449  CL58 HCT 116 C344(0.90)  LDD0766  [5]
 LDCM0450  CL59 HCT 116 C344(1.00)  LDD0767  [5]
 LDCM0451  CL6 HCT 116 C344(1.06)  LDD0768  [5]
 LDCM0452  CL60 HCT 116 C344(1.01)  LDD0769  [5]
 LDCM0453  CL61 HCT 116 C344(0.83); C604(1.04)  LDD0770  [5]
 LDCM0454  CL62 HCT 116 C344(0.82); C604(0.97)  LDD0771  [5]
 LDCM0455  CL63 HCT 116 C344(0.86); C604(0.92)  LDD0772  [5]
 LDCM0456  CL64 HCT 116 C344(0.90); C604(1.15)  LDD0773  [5]
 LDCM0457  CL65 HCT 116 C344(0.95); C604(1.00)  LDD0774  [5]
 LDCM0458  CL66 HCT 116 C344(0.90); C604(1.07)  LDD0775  [5]
 LDCM0459  CL67 HCT 116 C344(0.89); C604(1.14)  LDD0776  [5]
 LDCM0460  CL68 HCT 116 C344(0.75); C604(1.01)  LDD0777  [5]
 LDCM0461  CL69 HCT 116 C344(0.71); C604(1.01)  LDD0778  [5]
 LDCM0462  CL7 HCT 116 C344(1.03)  LDD0779  [5]
 LDCM0463  CL70 HCT 116 C344(0.84); C604(0.93)  LDD0780  [5]
 LDCM0464  CL71 HCT 116 C344(0.83); C604(0.83)  LDD0781  [5]
 LDCM0465  CL72 HCT 116 C344(0.61); C604(1.10)  LDD0782  [5]
 LDCM0466  CL73 HCT 116 C344(0.80); C604(0.89)  LDD0783  [5]
 LDCM0467  CL74 HCT 116 C344(0.79); C604(1.02)  LDD0784  [5]
 LDCM0469  CL76 HEK-293T C601(0.86); C603(0.86); C604(0.91)  LDD1067  [5]
 LDCM0470  CL77 HEK-293T C601(0.87); C603(0.87); C604(0.89)  LDD1068  [5]
 LDCM0471  CL78 HEK-293T C601(0.91); C603(0.91); C604(0.85)  LDD1069  [5]
 LDCM0472  CL79 HEK-293T C601(0.89); C603(0.89); C604(0.82)  LDD1070  [5]
 LDCM0473  CL8 HCT 116 C344(1.00)  LDD0790  [5]
 LDCM0474  CL80 HEK-293T C601(0.91); C603(0.91); C604(0.94)  LDD1072  [5]
 LDCM0475  CL81 HEK-293T C601(1.00); C603(1.00); C604(0.91)  LDD1073  [5]
 LDCM0476  CL82 HEK-293T C601(0.97); C603(0.97); C604(1.06)  LDD1074  [5]
 LDCM0477  CL83 HEK-293T C601(1.06); C603(1.06); C604(1.10)  LDD1075  [5]
 LDCM0478  CL84 HEK-293T C601(0.95); C603(0.95); C604(0.88)  LDD1076  [5]
 LDCM0479  CL85 HEK-293T C601(0.97); C603(0.97); C604(0.94)  LDD1077  [5]
 LDCM0480  CL86 HEK-293T C601(0.92); C603(0.92); C604(0.82)  LDD1078  [5]
 LDCM0481  CL87 HEK-293T C601(0.85); C603(0.85); C604(0.84)  LDD1079  [5]
 LDCM0482  CL88 HEK-293T C601(0.86); C603(0.86); C604(0.92)  LDD1080  [5]
 LDCM0483  CL89 HEK-293T C601(0.83); C603(0.83); C604(0.90)  LDD1081  [5]
 LDCM0484  CL9 HCT 116 C344(0.95)  LDD0801  [5]
 LDCM0485  CL90 HEK-293T C601(0.81); C603(0.81); C604(0.86)  LDD1083  [5]
 LDCM0486  CL91 HCT 116 C344(0.88)  LDD0803  [5]
 LDCM0487  CL92 HCT 116 C344(0.83)  LDD0804  [5]
 LDCM0488  CL93 HCT 116 C344(1.10)  LDD0805  [5]
 LDCM0489  CL94 HCT 116 C344(1.22)  LDD0806  [5]
 LDCM0490  CL95 HCT 116 C344(0.93)  LDD0807  [5]
 LDCM0491  CL96 HCT 116 C344(0.95)  LDD0808  [5]
 LDCM0492  CL97 HCT 116 C344(0.88)  LDD0809  [5]
 LDCM0493  CL98 HCT 116 C344(1.09)  LDD0810  [5]
 LDCM0494  CL99 HCT 116 C344(1.19)  LDD0811  [5]
 LDCM0495  E2913 HEK-293T C344(0.94); C1031(0.98); C659(1.36); C99(0.91)  LDD1698  [16]
 LDCM0625  F8 Ramos C936(3.13)  LDD2187  [17]
 LDCM0572  Fragment10 Ramos C936(1.68)  LDD2189  [17]
 LDCM0574  Fragment12 Ramos C936(1.18)  LDD2191  [17]
 LDCM0575  Fragment13 Ramos C936(1.71)  LDD2192  [17]
 LDCM0579  Fragment20 Ramos C936(1.22)  LDD2194  [17]
 LDCM0580  Fragment21 Ramos C936(1.03)  LDD2195  [17]
 LDCM0582  Fragment23 Ramos C936(1.21)  LDD2196  [17]
 LDCM0578  Fragment27 Ramos C936(1.20)  LDD2197  [17]
 LDCM0586  Fragment28 Ramos C936(0.68)  LDD2198  [17]
 LDCM0588  Fragment30 Ramos C936(2.35)  LDD2199  [17]
 LDCM0589  Fragment31 Ramos C936(1.78)  LDD2200  [17]
 LDCM0590  Fragment32 Ramos C936(2.39)  LDD2201  [17]
 LDCM0468  Fragment33 HCT 116 C344(0.78); C604(1.01)  LDD0785  [5]
 LDCM0596  Fragment38 Ramos C936(2.21)  LDD2203  [17]
 LDCM0427  Fragment51 HEK-293T C601(1.21); C604(1.61)  LDD1025  [5]
 LDCM0610  Fragment52 Ramos C936(1.45)  LDD2204  [17]
 LDCM0569  Fragment7 Ramos C936(1.26)  LDD2186  [17]
 LDCM0571  Fragment9 Ramos C936(1.15)  LDD2188  [17]
 LDCM0116  HHS-0101 DM93 Y71(0.87)  LDD0264  [3]
 LDCM0117  HHS-0201 DM93 Y71(1.19)  LDD0265  [3]
 LDCM0118  HHS-0301 DM93 Y71(2.02)  LDD0266  [3]
 LDCM0119  HHS-0401 DM93 Y71(1.39)  LDD0267  [3]
 LDCM0120  HHS-0701 DM93 Y71(1.72)  LDD0268  [3]
 LDCM0107  IAA HeLa N.A.  LDD0221  [4]
 LDCM0022  KB02 HCT 116 C604(1.04)  LDD0080  [5]
 LDCM0023  KB03 HCT 116 C604(1.13)  LDD0081  [5]
 LDCM0024  KB05 HCT 116 C604(0.95)  LDD0082  [5]
 LDCM0528  N-(4-bromophenyl)-2-cyano-N-phenylacetamide MDA-MB-231 C936(0.52)  LDD2121  [2]
 LDCM0109  NEM HeLa N.A.  LDD0224  [4]
 LDCM0496  Nucleophilic fragment 11a MDA-MB-231 C936(0.47)  LDD2089  [2]
 LDCM0500  Nucleophilic fragment 13a MDA-MB-231 C936(1.14)  LDD2093  [2]
 LDCM0506  Nucleophilic fragment 16a MDA-MB-231 C936(0.97)  LDD2099  [2]
 LDCM0514  Nucleophilic fragment 20a MDA-MB-231 C936(0.92)  LDD2107  [2]
 LDCM0516  Nucleophilic fragment 21a MDA-MB-231 C936(0.87)  LDD2109  [2]
 LDCM0518  Nucleophilic fragment 22a MDA-MB-231 C936(0.91)  LDD2111  [2]
 LDCM0530  Nucleophilic fragment 28a MDA-MB-231 C936(0.95)  LDD2123  [2]
 LDCM0532  Nucleophilic fragment 29a MDA-MB-231 C936(1.01)  LDD2125  [2]
 LDCM0534  Nucleophilic fragment 30a MDA-MB-231 C936(0.97)  LDD2127  [2]
 LDCM0536  Nucleophilic fragment 31 MDA-MB-231 C936(0.97)  LDD2129  [2]
 LDCM0542  Nucleophilic fragment 37 MDA-MB-231 C936(1.13)  LDD2135  [2]
 LDCM0211  Nucleophilic fragment 3b MDA-MB-231 C936(2.21)  LDD1700  [2]
 LDCM0546  Nucleophilic fragment 40 MDA-MB-231 C936(0.67)  LDD2140  [2]
 LDCM0552  Nucleophilic fragment 6a MDA-MB-231 C936(0.92)  LDD2146  [2]
 LDCM0021  THZ1 HCT 116 C604(1.04)  LDD2173  [5]

References

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2 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
3 Discovery of a Cell-Active SuTEx Ligand of Prostaglandin Reductase 2. Chembiochem. 2021 Jun 15;22(12):2134-2139. doi: 10.1002/cbic.202000879. Epub 2021 Apr 29.
4 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
5 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
6 Cyclopropenone, Cyclopropeniminium Ion, and Cyclopropenethione as Novel Electrophilic Warheads for Potential Target Discovery of Triple-Negative Breast Cancer. J Med Chem. 2023 Feb 23;66(4):2851-2864. doi: 10.1021/acs.jmedchem.2c01889. Epub 2023 Feb 10.
7 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
8 Comparison of Quantitative Mass Spectrometry Platforms for Monitoring Kinase ATP Probe Uptake in Lung Cancer. J Proteome Res. 2018 Jan 5;17(1):63-75. doi: 10.1021/acs.jproteome.7b00329. Epub 2017 Nov 22.
Mass spectrometry data entry: PXD006095 , PXD006096
9 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
10 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
11 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
12 Oxidant-Induced Bioconjugation for Protein Labeling in Live Cells. ACS Chem Biol. 2023 Jan 20;18(1):112-122. doi: 10.1021/acschembio.2c00740. Epub 2022 Dec 21.
13 Global targeting of functional tyrosines using sulfur-triazole exchange chemistry. Nat Chem Biol. 2020 Feb;16(2):150-159. doi: 10.1038/s41589-019-0404-5. Epub 2019 Nov 25.
14 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587
15 Ligand and Target Discovery by Fragment-Based Screening in Human Cells. Cell. 2017 Jan 26;168(3):527-541.e29. doi: 10.1016/j.cell.2016.12.029. Epub 2017 Jan 19.
16 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
17 Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry. Methods Enzymol. 2023;679:295-322. doi: 10.1016/bs.mie.2022.07.037. Epub 2022 Sep 7.
Mass spectrometry data entry: PXD027578