Details of the Target

General Information of Target

Target ID LDTP02883
Target Name Alpha-N-acetylgalactosaminidase (NAGA)
Gene Name NAGA
Gene ID 4668
Synonyms
Alpha-N-acetylgalactosaminidase; EC 3.2.1.49; Alpha-galactosidase B
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
Download
Sequence
MLLKTVLLLGHVAQVLMLDNGLLQTPPMGWLAWERFRCNINCDEDPKNCISEQLFMEMAD
RMAQDGWRDMGYTYLNIDDCWIGGRDASGRLMPDPKRFPHGIPFLADYVHSLGLKLGIYA
DMGNFTCMGYPGTTLDKVVQDAQTFAEWKVDMLKLDGCFSTPEERAQGYPKMAAALNATG
RPIAFSCSWPAYEGGLPPRVNYSLLADICNLWRNYDDIQDSWWSVLSILNWFVEHQDILQ
PVAGPGHWNDPDMLLIGNFGLSLEQSRAQMALWTVLAAPLLMSTDLRTISAQNMDILQNP
LMIKINQDPLGIQGRRIHKEKSLIEVYMRPLSNKASALVFFSCRTDMPYRYHSSLGQLNF
TGSVIYEAQDVYSGDIISGLRDETNFTVIINPSGVVMWYLYPIKNLEMSQQ
Target Bioclass
Enzyme
Family
Glycosyl hydrolase 27 family
Subcellular location
Lysosome
Function Removes terminal alpha-N-acetylgalactosamine residues from glycolipids and glycopeptides. Required for the breakdown of glycolipids.
Uniprot ID
P17050
Ensemble ID
ENST00000396398.8
HGNC ID
HGNC:7631
ChEMBL ID
CHEMBL3132

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 13 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
Alkylaryl probe 2
 Probe Info  		17.00  LDD0391  [1]
DBIA
 Probe Info  		C343(1.33)  LDD3314  [2]
FBPP2
 Probe Info  		2.40  LDD0054  [3]
Acrolein
 Probe Info  		N.A.  LDD0230  [4]
4-Iodoacetamidophenylacetylene
 Probe Info  		C343(0.00); C80(0.00); C158(0.00); C49(0.00)  LDD0038  [5]
IA-alkyne
 Probe Info  		C343(0.00); C158(0.00); C49(0.00)  LDD0036  [5]
Lodoacetamide azide
 Probe Info  		C343(0.00); C80(0.00); C158(0.00)  LDD0037  [5]
JW-RF-010
 Probe Info  		N.A.  LDD0026  [6]
IPM
 Probe Info  		C158(0.00); C38(0.00)  LDD2156  [7]
Crotonaldehyde
 Probe Info  		N.A.  LDD0219  [4]
Methacrolein
 Probe Info  		N.A.  LDD0218  [4]
AOyne
 Probe Info  		12.40  LDD0443  [8]
NAIA_5
 Probe Info  		N.A.  LDD2223  [9]
PAL-AfBPP Probe
Click To Hide/Show 1 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C220
 Probe Info  		12.91  LDD1894  [10]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0214  AC1 HEK-293T C158(0.84)  LDD1507  [11]
 LDCM0215  AC10 HEK-293T C158(1.13)  LDD1508  [11]
 LDCM0226  AC11 HEK-293T C209(0.95)  LDD1509  [11]
 LDCM0237  AC12 HEK-293T C158(0.98)  LDD1510  [11]
 LDCM0259  AC14 HEK-293T C158(1.08)  LDD1512  [11]
 LDCM0270  AC15 HEK-293T C158(1.16)  LDD1513  [11]
 LDCM0276  AC17 HEK-293T C158(1.00)  LDD1515  [11]
 LDCM0277  AC18 HEK-293T C158(0.92)  LDD1516  [11]
 LDCM0278  AC19 HEK-293T C209(0.70)  LDD1517  [11]
 LDCM0279  AC2 HEK-293T C158(0.95)  LDD1518  [11]
 LDCM0280  AC20 HEK-293T C158(0.91)  LDD1519  [11]
 LDCM0282  AC22 HEK-293T C158(1.02)  LDD1521  [11]
 LDCM0283  AC23 HEK-293T C158(0.97)  LDD1522  [11]
 LDCM0284  AC24 HEK-293T C158(1.05)  LDD1523  [11]
 LDCM0285  AC25 HEK-293T C158(0.93)  LDD1524  [11]
 LDCM0286  AC26 HEK-293T C158(0.89)  LDD1525  [11]
 LDCM0287  AC27 HEK-293T C209(0.83)  LDD1526  [11]
 LDCM0288  AC28 HEK-293T C158(0.95)  LDD1527  [11]
 LDCM0290  AC3 HEK-293T C209(0.99)  LDD1529  [11]
 LDCM0291  AC30 HEK-293T C158(1.16)  LDD1530  [11]
 LDCM0292  AC31 HEK-293T C158(1.18)  LDD1531  [11]
 LDCM0293  AC32 HEK-293T C158(1.04)  LDD1532  [11]
 LDCM0294  AC33 HEK-293T C158(0.88)  LDD1533  [11]
 LDCM0295  AC34 HEK-293T C158(0.93)  LDD1534  [11]
 LDCM0296  AC35 HEK-293T C209(1.03)  LDD1535  [11]
 LDCM0297  AC36 HEK-293T C158(1.11)  LDD1536  [11]
 LDCM0299  AC38 HEK-293T C158(1.00)  LDD1538  [11]
 LDCM0300  AC39 HEK-293T C158(1.19)  LDD1539  [11]
 LDCM0301  AC4 HEK-293T C158(0.94)  LDD1540  [11]
 LDCM0302  AC40 HEK-293T C158(1.08)  LDD1541  [11]
 LDCM0303  AC41 HEK-293T C158(0.95)  LDD1542  [11]
 LDCM0304  AC42 HEK-293T C158(0.86)  LDD1543  [11]
 LDCM0305  AC43 HEK-293T C209(0.65)  LDD1544  [11]
 LDCM0306  AC44 HEK-293T C158(0.97)  LDD1545  [11]
 LDCM0308  AC46 HEK-293T C158(0.94)  LDD1547  [11]
 LDCM0309  AC47 HEK-293T C158(1.01)  LDD1548  [11]
 LDCM0310  AC48 HEK-293T C158(1.04)  LDD1549  [11]
 LDCM0311  AC49 HEK-293T C158(0.97)  LDD1550  [11]
 LDCM0313  AC50 HEK-293T C158(0.84)  LDD1552  [11]
 LDCM0314  AC51 HEK-293T C209(0.68)  LDD1553  [11]
 LDCM0315  AC52 HEK-293T C158(0.92)  LDD1554  [11]
 LDCM0317  AC54 HEK-293T C158(1.10)  LDD1556  [11]
 LDCM0318  AC55 HEK-293T C158(1.18)  LDD1557  [11]
 LDCM0319  AC56 HEK-293T C158(1.04)  LDD1558  [11]
 LDCM0320  AC57 HEK-293T C158(0.89)  LDD1559  [11]
 LDCM0321  AC58 HEK-293T C158(0.95)  LDD1560  [11]
 LDCM0322  AC59 HEK-293T C209(0.89)  LDD1561  [11]
 LDCM0323  AC6 HEK-293T C158(0.99)  LDD1562  [11]
 LDCM0324  AC60 HEK-293T C158(1.01)  LDD1563  [11]
 LDCM0326  AC62 HEK-293T C158(1.13)  LDD1565  [11]
 LDCM0327  AC63 HEK-293T C158(0.93)  LDD1566  [11]
 LDCM0328  AC64 HEK-293T C158(1.06)  LDD1567  [11]
 LDCM0334  AC7 HEK-293T C158(0.88)  LDD1568  [11]
 LDCM0345  AC8 HEK-293T C158(1.01)  LDD1569  [11]
 LDCM0356  AKOS034007680 HEK-293T C158(1.04)  LDD1570  [11]
 LDCM0275  AKOS034007705 HEK-293T C158(1.06)  LDD1514  [11]
 LDCM0367  CL1 HEK-293T C158(1.35)  LDD1571  [11]
 LDCM0368  CL10 HEK-293T C158(1.26)  LDD1572  [11]
 LDCM0369  CL100 HEK-293T C158(0.96)  LDD1573  [11]
 LDCM0370  CL101 HEK-293T C158(1.24)  LDD1574  [11]
 LDCM0371  CL102 HEK-293T C158(0.99); C209(0.89)  LDD1575  [11]
 LDCM0372  CL103 HEK-293T C158(1.09)  LDD1576  [11]
 LDCM0373  CL104 HEK-293T C158(1.07)  LDD1577  [11]
 LDCM0374  CL105 HEK-293T C158(1.01)  LDD1578  [11]
 LDCM0375  CL106 HEK-293T C158(0.88); C209(0.75)  LDD1579  [11]
 LDCM0376  CL107 HEK-293T C158(1.08)  LDD1580  [11]
 LDCM0377  CL108 HEK-293T C158(1.06)  LDD1581  [11]
 LDCM0378  CL109 HEK-293T C158(1.05)  LDD1582  [11]
 LDCM0379  CL11 HEK-293T C158(1.15)  LDD1583  [11]
 LDCM0380  CL110 HEK-293T C158(1.06); C209(0.85)  LDD1584  [11]
 LDCM0381  CL111 HEK-293T C158(0.76)  LDD1585  [11]
 LDCM0382  CL112 HEK-293T C158(0.87)  LDD1586  [11]
 LDCM0383  CL113 HEK-293T C158(1.19)  LDD1587  [11]
 LDCM0384  CL114 HEK-293T C158(0.83); C209(0.77)  LDD1588  [11]
 LDCM0385  CL115 HEK-293T C158(0.88)  LDD1589  [11]
 LDCM0386  CL116 HEK-293T C158(0.92)  LDD1590  [11]
 LDCM0387  CL117 HEK-293T C158(1.27)  LDD1591  [11]
 LDCM0388  CL118 HEK-293T C158(0.98); C209(0.82)  LDD1592  [11]
 LDCM0389  CL119 HEK-293T C158(1.07)  LDD1593  [11]
 LDCM0390  CL12 HEK-293T C158(0.98)  LDD1594  [11]
 LDCM0391  CL120 HEK-293T C158(0.94)  LDD1595  [11]
 LDCM0392  CL121 HEK-293T C158(1.19)  LDD1596  [11]
 LDCM0393  CL122 HEK-293T C158(0.91); C209(0.78)  LDD1597  [11]
 LDCM0394  CL123 HEK-293T C158(0.90)  LDD1598  [11]
 LDCM0395  CL124 HEK-293T C158(1.02)  LDD1599  [11]
 LDCM0396  CL125 HEK-293T C158(1.10)  LDD1600  [11]
 LDCM0397  CL126 HEK-293T C158(0.88); C209(0.75)  LDD1601  [11]
 LDCM0398  CL127 HEK-293T C158(1.10)  LDD1602  [11]
 LDCM0399  CL128 HEK-293T C158(1.06)  LDD1603  [11]
 LDCM0400  CL13 HEK-293T C158(1.24)  LDD1604  [11]
 LDCM0401  CL14 HEK-293T C158(0.88); C209(0.77)  LDD1605  [11]
 LDCM0402  CL15 HEK-293T C158(1.06)  LDD1606  [11]
 LDCM0403  CL16 HEK-293T C158(1.10)  LDD1607  [11]
 LDCM0404  CL17 HEK-293T C158(1.35)  LDD1608  [11]
 LDCM0405  CL18 HEK-293T C158(1.11)  LDD1609  [11]
 LDCM0406  CL19 HEK-293T C209(1.03)  LDD1610  [11]
 LDCM0407  CL2 HEK-293T C158(1.14); C209(0.81)  LDD1611  [11]
 LDCM0408  CL20 HEK-293T C158(1.13)  LDD1612  [11]
 LDCM0410  CL22 HEK-293T C158(1.12)  LDD1614  [11]
 LDCM0411  CL23 HEK-293T C158(1.31)  LDD1615  [11]
 LDCM0412  CL24 HEK-293T C158(0.90)  LDD1616  [11]
 LDCM0413  CL25 HEK-293T C158(1.14)  LDD1617  [11]
 LDCM0414  CL26 HEK-293T C158(0.91); C209(0.74)  LDD1618  [11]
 LDCM0415  CL27 HEK-293T C158(0.73)  LDD1619  [11]
 LDCM0416  CL28 HEK-293T C158(0.98)  LDD1620  [11]
 LDCM0417  CL29 HEK-293T C158(1.04)  LDD1621  [11]
 LDCM0418  CL3 HEK-293T C158(0.98)  LDD1622  [11]
 LDCM0419  CL30 HEK-293T C158(0.87)  LDD1623  [11]
 LDCM0420  CL31 HEK-293T C209(1.12)  LDD1624  [11]
 LDCM0421  CL32 HEK-293T C158(1.07)  LDD1625  [11]
 LDCM0423  CL34 HEK-293T C158(1.35)  LDD1627  [11]
 LDCM0424  CL35 HEK-293T C158(1.35)  LDD1628  [11]
 LDCM0425  CL36 HEK-293T C158(0.93)  LDD1629  [11]
 LDCM0426  CL37 HEK-293T C158(0.98)  LDD1630  [11]
 LDCM0428  CL39 HEK-293T C158(1.32)  LDD1632  [11]
 LDCM0429  CL4 HEK-293T C158(0.97)  LDD1633  [11]
 LDCM0430  CL40 HEK-293T C158(0.92)  LDD1634  [11]
 LDCM0431  CL41 HEK-293T C158(0.91)  LDD1635  [11]
 LDCM0432  CL42 HEK-293T C158(1.05)  LDD1636  [11]
 LDCM0433  CL43 HEK-293T C209(2.08)  LDD1637  [11]
 LDCM0434  CL44 HEK-293T C158(1.13)  LDD1638  [11]
 LDCM0436  CL46 HEK-293T C158(1.21)  LDD1640  [11]
 LDCM0437  CL47 HEK-293T C158(1.29)  LDD1641  [11]
 LDCM0438  CL48 HEK-293T C158(0.89)  LDD1642  [11]
 LDCM0439  CL49 HEK-293T C158(0.99)  LDD1643  [11]
 LDCM0440  CL5 HEK-293T C158(0.95)  LDD1644  [11]
 LDCM0441  CL50 HEK-293T C158(0.87); C209(0.74)  LDD1645  [11]
 LDCM0443  CL52 HEK-293T C158(0.95)  LDD1646  [11]
 LDCM0444  CL53 HEK-293T C158(1.03)  LDD1647  [11]
 LDCM0445  CL54 HEK-293T C158(0.84)  LDD1648  [11]
 LDCM0446  CL55 HEK-293T C209(1.49)  LDD1649  [11]
 LDCM0447  CL56 HEK-293T C158(1.08)  LDD1650  [11]
 LDCM0449  CL58 HEK-293T C158(1.21)  LDD1652  [11]
 LDCM0450  CL59 HEK-293T C158(1.35)  LDD1653  [11]
 LDCM0451  CL6 HEK-293T C158(1.09)  LDD1654  [11]
 LDCM0452  CL60 HEK-293T C158(0.98)  LDD1655  [11]
 LDCM0453  CL61 HEK-293T C158(1.02)  LDD1656  [11]
 LDCM0454  CL62 HEK-293T C158(0.95); C209(0.87)  LDD1657  [11]
 LDCM0455  CL63 HEK-293T C158(0.82)  LDD1658  [11]
 LDCM0456  CL64 HEK-293T C158(1.01)  LDD1659  [11]
 LDCM0457  CL65 HEK-293T C158(1.11)  LDD1660  [11]
 LDCM0458  CL66 HEK-293T C158(0.96)  LDD1661  [11]
 LDCM0459  CL67 HEK-293T C209(1.10)  LDD1662  [11]
 LDCM0460  CL68 HEK-293T C158(1.02)  LDD1663  [11]
 LDCM0462  CL7 HEK-293T C209(0.87)  LDD1665  [11]
 LDCM0463  CL70 HEK-293T C158(1.07)  LDD1666  [11]
 LDCM0464  CL71 HEK-293T C158(1.01)  LDD1667  [11]
 LDCM0465  CL72 HEK-293T C158(0.96)  LDD1668  [11]
 LDCM0466  CL73 HEK-293T C158(1.26)  LDD1669  [11]
 LDCM0467  CL74 HEK-293T C158(0.86); C209(0.81)  LDD1670  [11]
 LDCM0469  CL76 HEK-293T C158(1.00)  LDD1672  [11]
 LDCM0470  CL77 HEK-293T C158(0.90)  LDD1673  [11]
 LDCM0471  CL78 HEK-293T C158(1.01)  LDD1674  [11]
 LDCM0472  CL79 HEK-293T C209(0.77)  LDD1675  [11]
 LDCM0473  CL8 HEK-293T C158(0.86)  LDD1676  [11]
 LDCM0474  CL80 HEK-293T C158(1.07)  LDD1677  [11]
 LDCM0476  CL82 HEK-293T C158(1.00)  LDD1679  [11]
 LDCM0477  CL83 HEK-293T C158(1.42)  LDD1680  [11]
 LDCM0478  CL84 HEK-293T C158(1.03)  LDD1681  [11]
 LDCM0479  CL85 HEK-293T C158(1.04)  LDD1682  [11]
 LDCM0480  CL86 HEK-293T C158(0.88); C209(0.71)  LDD1683  [11]
 LDCM0481  CL87 HEK-293T C158(0.96)  LDD1684  [11]
 LDCM0482  CL88 HEK-293T C158(0.94)  LDD1685  [11]
 LDCM0483  CL89 HEK-293T C158(1.17)  LDD1686  [11]
 LDCM0485  CL90 HEK-293T C158(1.28)  LDD1688  [11]
 LDCM0486  CL91 HEK-293T C209(1.24)  LDD1689  [11]
 LDCM0487  CL92 HEK-293T C158(1.03)  LDD1690  [11]
 LDCM0489  CL94 HEK-293T C158(1.14)  LDD1692  [11]
 LDCM0490  CL95 HEK-293T C158(1.12)  LDD1693  [11]
 LDCM0491  CL96 HEK-293T C158(1.11)  LDD1694  [11]
 LDCM0492  CL97 HEK-293T C158(1.09)  LDD1695  [11]
 LDCM0493  CL98 HEK-293T C158(1.08); C209(0.91)  LDD1696  [11]
 LDCM0494  CL99 HEK-293T C158(0.82)  LDD1697  [11]
 LDCM0495  E2913 HEK-293T C158(0.92)  LDD1698  [11]
 LDCM0468  Fragment33 HEK-293T C158(0.83)  LDD1671  [11]
 LDCM0427  Fragment51 HEK-293T C158(0.87); C209(0.79)  LDD1631  [11]
 LDCM0022  KB02 42-MG-BA C343(1.16)  LDD2244  [2]
 LDCM0023  KB03 42-MG-BA C343(1.12)  LDD2661  [2]
 LDCM0024  KB05 IGR37 C343(1.33)  LDD3314  [2]
 LDCM0109  NEM HeLa N.A.  LDD0230  [4]
 LDCM0099  Phenelzine MDA-MB-231 3.00  LDD0392  [1]
 LDCM0008  Tranylcypromine SH-SY5Y 2.40  LDD0054  [3]

The Interaction Atlas With This Target

The Drug(s) Related To This Target

Approved
Click To Hide/Show 1 Drug(s) Interacting with This Target
Drug Name Drug Type External ID
Glycerin . DB09462

References

1 Hydrazines as versatile chemical biology probes and drug-discovery tools for cofactor-dependent enzymes. bioRxiv, 2020-06.
2 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
3 Tranylcypromine specificity for monoamine oxidase is limited by promiscuous protein labelling and lysosomal trapping. RSC Chem Biol. 2020 Aug 12;1(4):209-213. doi: 10.1039/d0cb00048e. eCollection 2020 Oct 1.
Mass spectrometry data entry: PXD018580
4 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
5 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
6 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
7 Benchmarking Cleavable Biotin Tags for Peptide-Centric Chemoproteomics. J Proteome Res. 2022 May 6;21(5):1349-1358. doi: 10.1021/acs.jproteome.2c00174. Epub 2022 Apr 25.
Mass spectrometry data entry: PXD031019
8 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
9 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
10 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587
11 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402