General Information of Target

Target ID LDTP01246
Target Name KH domain-containing, RNA-binding, signal transduction-associated protein 3 (KHDRBS3)
Gene Name KHDRBS3
Gene ID 10656
Synonyms
SALP; SLM2; KH domain-containing, RNA-binding, signal transduction-associated protein 3; RNA-binding protein T-Star; Sam68-like mammalian protein 2; SLM-2; Sam68-like phosphotyrosine protein
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MEEKYLPELMAEKDSLDPSFTHALRLVNQEIEKFQKGEGKDEEKYIDVVINKNMKLGQKV
LIPVKQFPKFNFVGKLLGPRGNSLKRLQEETLTKMSILGKGSMRDKAKEEELRKSGEAKY
FHLNDDLHVLIEVFAPPAEAYARMGHALEEIKKFLIPDYNDEIRQAQLQELTYLNGGSEN
ADVPVVRGKPTLRTRGVPAPAITRGRGGVTARPVGVVVPRGTPTPRGVLSTRGPVSRGRG
LLTPRARGVPPTGYRPPPPPPTQETYGEYDYDDGYGTAYDEQSYDSYDNSYSTPAQSGAD
YYDYGHGLSEETYDSYGQEEWTNSRHKAPSARTAKGVYRDQPYGRY
Target Bioclass
Other
Family
KHDRBS family
Subcellular location
Nucleus
Function
RNA-binding protein that plays a role in the regulation of alternative splicing and influences mRNA splice site selection and exon inclusion. Binds preferentially to the 5'-[AU]UAAA-3' motif in vitro. Binds optimally to RNA containing 5'-[AU]UAA-3' as a bipartite motif spaced by more than 15 nucleotides. Binds poly(A). RNA-binding abilities are down-regulated by tyrosine kinase PTK6. Involved in splice site selection of vascular endothelial growth factor. In vitro regulates CD44 alternative splicing by direct binding to purine-rich exonic enhancer. Can regulate alternative splicing of neurexins NRXN1-3 in the laminin G-like domain 6 containing the evolutionary conserved neurexin alternative spliced segment 4 (AS4) involved in neurexin selective targeting to postsynaptic partners such as neuroligins and LRRTM family members. Targeted, cell-type specific splicing regulation of NRXN1 at AS4 is involved in neuronal glutamatergic synapse function and plasticity. May regulate expression of KHDRBS2/SLIM-1 in defined brain neuron populations by modifying its alternative splicing. Can bind FABP9 mRNA. May play a role as a negative regulator of cell growth. Inhibits cell proliferation.; (Microbial infection) Involved in post-transcriptional regulation of HIV-1 gene expression.
Uniprot ID
O75525
Ensemble ID
ENST00000355849.10
HGNC ID
HGNC:18117

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
HEC1 SNV: p.A138V .
HEC1B SNV: p.A138V .
HS936T SNV: p.R204K .
KYSE30 SNV: p.E12G .
MEWO SNV: p.E311K .
NCIH1650 SNV: p.E320Q .
SNU1 SNV: p.V64L .
SW1271 SNV: p.G227E .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 3 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
HHS-475
 Probe Info 
Y5(0.85)  LDD0264  [1]
ATP probe
 Probe Info 
N.A.  LDD0199  [2]
HHS-465
 Probe Info 
N.A.  LDD2240  [3]
PAL-AfBPP Probe
Click To Hide/Show 9 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C139
 Probe Info 
11.39  LDD1821  [4]
C165
 Probe Info 
16.22  LDD1845  [4]
C293
 Probe Info 
17.15  LDD1963  [4]
C296
 Probe Info 
11.88  LDD1966  [4]
C313
 Probe Info 
14.42  LDD1980  [4]
C314
 Probe Info 
12.38  LDD1981  [4]
C346
 Probe Info 
11.39  LDD2007  [4]
C355
 Probe Info 
23.43  LDD2016  [4]
C383
 Probe Info 
13.93  LDD2042  [4]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0116  HHS-0101 DM93 Y5(0.85)  LDD0264  [1]
 LDCM0117  HHS-0201 DM93 Y5(1.05)  LDD0265  [1]
 LDCM0118  HHS-0301 DM93 Y5(1.02)  LDD0266  [1]
 LDCM0119  HHS-0401 DM93 Y5(0.99)  LDD0267  [1]
 LDCM0120  HHS-0701 DM93 Y5(1.05)  LDD0268  [1]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 5 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Replication factor C subunit 3 (RFC3) Activator 1 small subunits family P40938
Phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 1 (INPP5D) Inositol 1,4,5-trisphosphate 5-phosphatase family Q92835
Proteasome subunit alpha type-1 (PSMA1) Peptidase T1A family P25786
Non-receptor tyrosine-protein kinase TYK2 (TYK2) Tyr protein kinase family P29597
Dexamethasone-induced Ras-related protein 1 (RASD1) RasD family Q9Y272
Transporter and channel
Click To Hide/Show 4 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Metal transporter CNNM3 (CNNM3) ACDP family Q8NE01
Aquaporin-1 (AQP1) MIP/aquaporin (TC 1.A.8) family P29972
Sorting nexin-18 (SNX18) Sorting nexin family Q96RF0
SH3 and multiple ankyrin repeat domains protein 3 (SHANK3) . Q9BYB0
Transcription factor
Click To Hide/Show 5 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Doublesex- and mab-3-related transcription factor 3 (DMRT3) DMRT family Q9NQL9
Zinc finger protein 575 (ZNF575) Krueppel C2H2-type zinc-finger protein family Q86XF7
Myb-related transcription factor, partner of profilin (MYPOP) . Q86VE0
Zinc finger protein 408 (ZNF408) . Q9H9D4
Zinc finger protein 580 (ZNF580) . Q9UK33
Other
Click To Hide/Show 16 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
DBIRD complex subunit ZNF326 (ZNF326) AKAP95 family Q5BKZ1
Dedicator of cytokinesis protein 2 (DOCK2) DOCK family Q92608
Hemoglobin subunit zeta (HBZ) Globin family P02008
KH domain-containing, RNA-binding, signal transduction-associated protein 2 (KHDRBS2) KHDRBS family Q5VWX1
Negative elongation factor B (NELFB) NELF-B family Q8WX92
U4/U6 small nuclear ribonucleoprotein Prp31 (PRPF31) PRP31 family Q8WWY3
Bromo adjacent homology domain-containing 1 protein (BAHD1) . Q8TBE0
Lamin tail domain-containing protein 2 (LMNTD2) . Q8IXW0
Proline-rich protein 3 (PRR3) . P79522
Protocadherin beta-14 (PCDHB14) . Q9Y5E9
Rab effector Noc2 (RPH3AL) . Q9UNE2
RAS protein activator like-3 (RASAL3) . Q86YV0
RNA-binding motif protein, X chromosome (RBMX) . P38159
RNA-binding protein 3 (RBM3) . P98179
Testis-specific protein 10-interacting protein (TSGA10IP) . Q3SY00
YTH domain-containing protein 1 (YTHDC1) . Q96MU7

References

1 Discovery of a Cell-Active SuTEx Ligand of Prostaglandin Reductase 2. Chembiochem. 2021 Jun 15;22(12):2134-2139. doi: 10.1002/cbic.202000879. Epub 2021 Apr 29.
2 Targeted Proteomic Approaches for Proteome-Wide Characterizations of the AMP-Binding Capacities of Kinases. J Proteome Res. 2022 Aug 5;21(8):2063-2070. doi: 10.1021/acs.jproteome.2c00225. Epub 2022 Jul 12.
3 Global profiling identifies a stress-responsive tyrosine site on EDC3 regulating biomolecular condensate formation. Cell Chem Biol. 2022 Dec 15;29(12):1709-1720.e7. doi: 10.1016/j.chembiol.2022.11.008. Epub 2022 Dec 6.
Mass spectrometry data entry: PXD038010
4 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587