Details of the Target

General Information of Target

Target ID LDTP00873
Target Name Cleavage and polyadenylation specificity factor subunit 5 (NUDT21)
Gene Name NUDT21
Gene ID 11051
Synonyms
CFIM25; CPSF25; CPSF5; Cleavage and polyadenylation specificity factor subunit 5; Cleavage and polyadenylation specificity factor 25 kDa subunit; CPSF 25 kDa subunit; Cleavage factor Im complex 25 kDa subunit; CFIm25; Nucleoside diphosphate-linked moiety X motif 21; Nudix motif 21; Nudix hydrolase 21; Pre-mRNA cleavage factor Im 68 kDa subunit
3D Structure
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2D Sequence (FASTA)
Download
3D Structure (PDB)
Download
Sequence
MSVVPPNRSQTGWPRGVTQFGNKYIQQTKPLTLERTINLYPLTNYTFGTKEPLYEKDSSV
AARFQRMREEFDKIGMRRTVEGVLIVHEHRLPHVLLLQLGTTFFKLPGGELNPGEDEVEG
LKRLMTEILGRQDGVLQDWVIDDCIGNWWRPNFEPPQYPYIPAHITKPKEHKKLFLVQLQ
EKALFAVPKNYKLVAAPLFELYDNAPGYGPIISSLPQLLSRFNFIYN
Target Bioclass
Enzyme
Family
Nudix hydrolase family, CPSF5 subfamily
Subcellular location
Nucleus
Function
Component of the cleavage factor Im (CFIm) complex that functions as an activator of the pre-mRNA 3'-end cleavage and polyadenylation processing required for the maturation of pre-mRNA into functional mRNAs. CFIm contributes to the recruitment of multiprotein complexes on specific sequences on the pre-mRNA 3'-end, so called cleavage and polyadenylation signals (pA signals). Most pre-mRNAs contain multiple pA signals, resulting in alternative cleavage and polyadenylation (APA) producing mRNAs with variable 3'-end formation. The CFIm complex acts as a key regulator of cleavage and polyadenylation site choice during APA through its binding to 5'-UGUA-3' elements localized in the 3'-untranslated region (UTR) for a huge number of pre-mRNAs. NUDT21/CPSF5 activates indirectly the mRNA 3'-processing machinery by recruiting CPSF6 and/or CPSF7. Binds to 5'-UGUA-3' elements localized upstream of pA signals that act as enhancers of pre-mRNA 3'-end processing . The homodimer mediates simultaneous sequence-specific recognition of two 5'-UGUA-3' elements within the pre-mRNA. Plays a role in somatic cell fate transitions and pluripotency by regulating widespread changes in gene expression through an APA-dependent function. Binds to chromatin. Binds to, but does not hydrolyze mono- and di-adenosine nucleotides.
Uniprot ID
O43809
Ensemble ID
ENST00000300291.10
HGNC ID
HGNC:13870

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 20 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
TH211
 Probe Info  		Y24(20.00)  LDD0257  [1]
TH216
 Probe Info  		Y24(20.00)  LDD0259  [1]
STPyne
 Probe Info  		K189(9.23); K23(6.60); K29(4.19)  LDD0277  [2]
Probe 1
 Probe Info  		Y54(28.51)  LDD3495  [3]
HHS-482
 Probe Info  		Y24(1.00)  LDD0285  [4]
HHS-475
 Probe Info  		Y24(0.93)  LDD0264  [5]
HHS-465
 Probe Info  		Y24(4.91)  LDD2237  [6]
DBIA
 Probe Info  		C144(0.61)  LDD0532  [7]
ATP probe
 Probe Info  		N.A.  LDD0199  [8]
4-Iodoacetamidophenylacetylene
 Probe Info  		N.A.  LDD0038  [9]
IA-alkyne
 Probe Info  		N.A.  LDD0036  [9]
IPIAA_H
 Probe Info  		N.A.  LDD0030  [10]
IPIAA_L
 Probe Info  		N.A.  LDD0031  [10]
Lodoacetamide azide
 Probe Info  		N.A.  LDD0037  [9]
NHS
 Probe Info  		N.A.  LDD0010  [11]
SF
 Probe Info  		K189(0.00); Y24(0.00)  LDD0028  [12]
1d-yne
 Probe Info  		N.A.  LDD0357  [13]
Acrolein
 Probe Info  		N.A.  LDD0217  [14]
AOyne
 Probe Info  		4.70  LDD0443  [15]
TER-AC
 Probe Info  		N.A.  LDD0426  [16]
PAL-AfBPP Probe
Click To Hide/Show 2 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
FFF probe13
 Probe Info  		12.24  LDD0475  [17]
FFF probe2
 Probe Info  		6.49  LDD0463  [17]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0215  AC10 HCT 116 C144(0.61)  LDD0532  [7]
 LDCM0226  AC11 HCT 116 C144(0.55)  LDD0543  [7]
 LDCM0237  AC12 HCT 116 C144(0.66)  LDD0554  [7]
 LDCM0259  AC14 HCT 116 C144(0.67)  LDD0576  [7]
 LDCM0263  AC143 HCT 116 C144(0.77)  LDD0580  [7]
 LDCM0264  AC144 HCT 116 C144(1.05)  LDD0581  [7]
 LDCM0265  AC145 HCT 116 C144(1.06)  LDD0582  [7]
 LDCM0266  AC146 HCT 116 C144(1.02)  LDD0583  [7]
 LDCM0267  AC147 HCT 116 C144(1.14)  LDD0584  [7]
 LDCM0268  AC148 HCT 116 C144(1.55)  LDD0585  [7]
 LDCM0269  AC149 HCT 116 C144(1.28)  LDD0586  [7]
 LDCM0270  AC15 HCT 116 C144(0.42)  LDD0587  [7]
 LDCM0271  AC150 HCT 116 C144(0.89)  LDD0588  [7]
 LDCM0272  AC151 HCT 116 C144(1.03)  LDD0589  [7]
 LDCM0273  AC152 HCT 116 C144(1.20)  LDD0590  [7]
 LDCM0274  AC153 HCT 116 C144(1.84)  LDD0591  [7]
 LDCM0621  AC154 HCT 116 C144(0.99)  LDD2158  [7]
 LDCM0622  AC155 HCT 116 C144(1.01)  LDD2159  [7]
 LDCM0623  AC156 HCT 116 C144(0.86)  LDD2160  [7]
 LDCM0624  AC157 HCT 116 C144(0.79)  LDD2161  [7]
 LDCM0276  AC17 HCT 116 C144(1.96)  LDD0593  [7]
 LDCM0277  AC18 HCT 116 C144(0.91)  LDD0594  [7]
 LDCM0278  AC19 HCT 116 C144(0.79)  LDD0595  [7]
 LDCM0279  AC2 HEK-293T C144(0.99)  LDD1518  [18]
 LDCM0280  AC20 HCT 116 C144(1.32)  LDD0597  [7]
 LDCM0281  AC21 HCT 116 C144(1.81)  LDD0598  [7]
 LDCM0282  AC22 HCT 116 C144(1.12)  LDD0599  [7]
 LDCM0283  AC23 HCT 116 C144(1.30)  LDD0600  [7]
 LDCM0284  AC24 HCT 116 C144(1.21)  LDD0601  [7]
 LDCM0286  AC26 HEK-293T C144(1.04)  LDD1525  [18]
 LDCM0287  AC27 HEK-293T C144(0.78)  LDD1526  [18]
 LDCM0288  AC28 HEK-293T C144(1.02)  LDD1527  [18]
 LDCM0289  AC29 HEK-293T C144(0.96)  LDD1528  [18]
 LDCM0290  AC3 HEK-293T C144(1.13)  LDD1529  [18]
 LDCM0291  AC30 HEK-293T C144(0.99)  LDD1530  [18]
 LDCM0292  AC31 HEK-293T C144(1.04)  LDD1531  [18]
 LDCM0293  AC32 HEK-293T C144(1.04)  LDD1532  [18]
 LDCM0295  AC34 HEK-293T C144(0.80)  LDD1534  [18]
 LDCM0296  AC35 HEK-293T C144(0.92)  LDD1535  [18]
 LDCM0297  AC36 HEK-293T C144(0.97)  LDD1536  [18]
 LDCM0298  AC37 HEK-293T C144(1.06)  LDD1537  [18]
 LDCM0299  AC38 HEK-293T C144(1.00)  LDD1538  [18]
 LDCM0300  AC39 HEK-293T C144(1.15)  LDD1539  [18]
 LDCM0301  AC4 HEK-293T C144(1.05)  LDD1540  [18]
 LDCM0302  AC40 HEK-293T C144(1.15)  LDD1541  [18]
 LDCM0304  AC42 HEK-293T C144(0.93)  LDD1543  [18]
 LDCM0305  AC43 HEK-293T C144(0.91)  LDD1544  [18]
 LDCM0306  AC44 HEK-293T C144(1.03)  LDD1545  [18]
 LDCM0307  AC45 HEK-293T C144(1.08)  LDD1546  [18]
 LDCM0308  AC46 HCT 116 C144(1.10)  LDD0625  [7]
 LDCM0309  AC47 HCT 116 C144(0.94)  LDD0626  [7]
 LDCM0310  AC48 HCT 116 C144(1.19)  LDD0627  [7]
 LDCM0311  AC49 HCT 116 C144(1.59)  LDD0628  [7]
 LDCM0312  AC5 HEK-293T C144(0.98)  LDD1551  [18]
 LDCM0313  AC50 HCT 116 C144(1.35)  LDD0630  [7]
 LDCM0314  AC51 HCT 116 C144(0.89)  LDD0631  [7]
 LDCM0315  AC52 HCT 116 C144(1.11)  LDD0632  [7]
 LDCM0316  AC53 HCT 116 C144(1.13)  LDD0633  [7]
 LDCM0317  AC54 HCT 116 C144(1.47)  LDD0634  [7]
 LDCM0318  AC55 HCT 116 C144(1.55)  LDD0635  [7]
 LDCM0319  AC56 HCT 116 C144(1.45)  LDD0636  [7]
 LDCM0320  AC57 HCT 116 C144(2.49)  LDD0637  [7]
 LDCM0321  AC58 HCT 116 C144(1.27)  LDD0638  [7]
 LDCM0322  AC59 HCT 116 C144(0.99)  LDD0639  [7]
 LDCM0323  AC6 HCT 116 C144(0.51)  LDD0640  [7]
 LDCM0324  AC60 HCT 116 C144(1.11)  LDD0641  [7]
 LDCM0325  AC61 HCT 116 C144(1.24)  LDD0642  [7]
 LDCM0326  AC62 HCT 116 C144(1.08)  LDD0643  [7]
 LDCM0327  AC63 HCT 116 C144(1.30)  LDD0644  [7]
 LDCM0328  AC64 HCT 116 C144(1.52)  LDD0645  [7]
 LDCM0329  AC65 HCT 116 C144(1.23)  LDD0646  [7]
 LDCM0330  AC66 HCT 116 C144(1.76)  LDD0647  [7]
 LDCM0331  AC67 HCT 116 C144(1.14)  LDD0648  [7]
 LDCM0332  AC68 HCT 116 C144(0.71)  LDD0649  [7]
 LDCM0333  AC69 HCT 116 C144(0.68)  LDD0650  [7]
 LDCM0334  AC7 HCT 116 C144(0.70)  LDD0651  [7]
 LDCM0335  AC70 HCT 116 C144(0.49)  LDD0652  [7]
 LDCM0336  AC71 HCT 116 C144(0.89)  LDD0653  [7]
 LDCM0337  AC72 HCT 116 C144(0.81)  LDD0654  [7]
 LDCM0338  AC73 HCT 116 C144(0.37)  LDD0655  [7]
 LDCM0339  AC74 HCT 116 C144(0.49)  LDD0656  [7]
 LDCM0340  AC75 HCT 116 C144(0.22)  LDD0657  [7]
 LDCM0341  AC76 HCT 116 C144(0.89)  LDD0658  [7]
 LDCM0342  AC77 HCT 116 C144(1.15)  LDD0659  [7]
 LDCM0343  AC78 HCT 116 C144(0.83)  LDD0660  [7]
 LDCM0344  AC79 HCT 116 C144(1.83)  LDD0661  [7]
 LDCM0345  AC8 HCT 116 C144(0.52)  LDD0662  [7]
 LDCM0346  AC80 HCT 116 C144(1.99)  LDD0663  [7]
 LDCM0347  AC81 HCT 116 C144(0.25)  LDD0664  [7]
 LDCM0348  AC82 HCT 116 C144(0.13)  LDD0665  [7]
 LDCM0248  AKOS034007472 HCT 116 C144(0.77)  LDD0565  [7]
 LDCM0356  AKOS034007680 HCT 116 C144(0.83)  LDD0673  [7]
 LDCM0275  AKOS034007705 HCT 116 C144(0.45)  LDD0592  [7]
 LDCM0108  Chloroacetamide HeLa N.A.  LDD0222  [14]
 LDCM0367  CL1 HEK-293T C144(0.81)  LDD1571  [18]
 LDCM0368  CL10 HEK-293T C144(0.91)  LDD1572  [18]
 LDCM0370  CL101 HCT 116 C144(0.55)  LDD0687  [7]
 LDCM0371  CL102 HCT 116 C144(0.60)  LDD0688  [7]
 LDCM0372  CL103 HCT 116 C144(0.56)  LDD0689  [7]
 LDCM0373  CL104 HCT 116 C144(0.66)  LDD0690  [7]
 LDCM0374  CL105 HCT 116 C144(1.28)  LDD0691  [7]
 LDCM0375  CL106 HCT 116 C144(0.64)  LDD0692  [7]
 LDCM0376  CL107 HCT 116 C144(0.72)  LDD0693  [7]
 LDCM0377  CL108 HCT 116 C144(0.79)  LDD0694  [7]
 LDCM0378  CL109 HCT 116 C144(1.02)  LDD0695  [7]
 LDCM0379  CL11 HEK-293T C144(1.29)  LDD1583  [18]
 LDCM0380  CL110 HCT 116 C144(0.70)  LDD0697  [7]
 LDCM0381  CL111 HCT 116 C144(1.10)  LDD0698  [7]
 LDCM0383  CL113 HEK-293T C144(0.72)  LDD1587  [18]
 LDCM0384  CL114 HEK-293T C144(0.81)  LDD1588  [18]
 LDCM0387  CL117 HEK-293T C144(0.96)  LDD1591  [18]
 LDCM0388  CL118 HEK-293T C144(0.98)  LDD1592  [18]
 LDCM0390  CL12 HEK-293T C144(1.21)  LDD1594  [18]
 LDCM0392  CL121 HCT 116 C144(1.21)  LDD0709  [7]
 LDCM0393  CL122 HCT 116 C144(1.38)  LDD0710  [7]
 LDCM0394  CL123 HCT 116 C144(1.29)  LDD0711  [7]
 LDCM0395  CL124 HCT 116 C144(1.52)  LDD0712  [7]
 LDCM0396  CL125 HCT 116 C144(1.26)  LDD0713  [7]
 LDCM0397  CL126 HCT 116 C144(0.77)  LDD0714  [7]
 LDCM0398  CL127 HCT 116 C144(1.01)  LDD0715  [7]
 LDCM0399  CL128 HCT 116 C144(1.62)  LDD0716  [7]
 LDCM0400  CL13 HEK-293T C144(0.95)  LDD1604  [18]
 LDCM0401  CL14 HEK-293T C144(1.11)  LDD1605  [18]
 LDCM0405  CL18 HEK-293T C144(0.99)  LDD1609  [18]
 LDCM0406  CL19 HEK-293T C144(1.46)  LDD1610  [18]
 LDCM0407  CL2 HEK-293T C144(1.07)  LDD1611  [18]
 LDCM0408  CL20 HEK-293T C144(1.48)  LDD1612  [18]
 LDCM0409  CL21 HEK-293T C144(0.96)  LDD1613  [18]
 LDCM0410  CL22 HEK-293T C144(1.12)  LDD1614  [18]
 LDCM0411  CL23 HEK-293T C144(1.40)  LDD1615  [18]
 LDCM0412  CL24 HEK-293T C144(1.12)  LDD1616  [18]
 LDCM0413  CL25 HEK-293T C144(0.90)  LDD1617  [18]
 LDCM0414  CL26 HEK-293T C144(0.99)  LDD1618  [18]
 LDCM0419  CL30 HEK-293T C144(0.91)  LDD1623  [18]
 LDCM0420  CL31 HEK-293T C144(1.02)  LDD1624  [18]
 LDCM0421  CL32 HEK-293T C144(1.11)  LDD1625  [18]
 LDCM0422  CL33 HEK-293T C144(0.83)  LDD1626  [18]
 LDCM0423  CL34 HEK-293T C144(1.02)  LDD1627  [18]
 LDCM0424  CL35 HEK-293T C144(1.16)  LDD1628  [18]
 LDCM0425  CL36 HEK-293T C144(1.29)  LDD1629  [18]
 LDCM0426  CL37 HEK-293T C144(1.09)  LDD1630  [18]
 LDCM0432  CL42 HEK-293T C144(0.86)  LDD1636  [18]
 LDCM0433  CL43 HEK-293T C144(1.06)  LDD1637  [18]
 LDCM0434  CL44 HEK-293T C144(0.89)  LDD1638  [18]
 LDCM0435  CL45 HEK-293T C144(1.32)  LDD1639  [18]
 LDCM0436  CL46 HEK-293T C144(1.08)  LDD1640  [18]
 LDCM0437  CL47 HEK-293T C144(1.14)  LDD1641  [18]
 LDCM0438  CL48 HEK-293T C144(1.25)  LDD1642  [18]
 LDCM0439  CL49 HEK-293T C144(0.92)  LDD1643  [18]
 LDCM0441  CL50 HEK-293T C144(0.99)  LDD1645  [18]
 LDCM0445  CL54 HEK-293T C144(0.65)  LDD1648  [18]
 LDCM0446  CL55 HEK-293T C144(1.05)  LDD1649  [18]
 LDCM0447  CL56 HEK-293T C144(1.01)  LDD1650  [18]
 LDCM0448  CL57 HEK-293T C144(1.02)  LDD1651  [18]
 LDCM0449  CL58 HEK-293T C144(1.38)  LDD1652  [18]
 LDCM0450  CL59 HEK-293T C144(1.19)  LDD1653  [18]
 LDCM0451  CL6 HEK-293T C144(0.87)  LDD1654  [18]
 LDCM0452  CL60 HEK-293T C144(1.00)  LDD1655  [18]
 LDCM0453  CL61 HEK-293T C144(0.90)  LDD1656  [18]
 LDCM0454  CL62 HEK-293T C144(1.22)  LDD1657  [18]
 LDCM0458  CL66 HEK-293T C144(0.78)  LDD1661  [18]
 LDCM0459  CL67 HEK-293T C144(1.18)  LDD1662  [18]
 LDCM0460  CL68 HEK-293T C144(1.38)  LDD1663  [18]
 LDCM0461  CL69 HEK-293T C144(0.79)  LDD1664  [18]
 LDCM0462  CL7 HEK-293T C144(1.34)  LDD1665  [18]
 LDCM0463  CL70 HEK-293T C144(1.16)  LDD1666  [18]
 LDCM0464  CL71 HEK-293T C144(1.13)  LDD1667  [18]
 LDCM0465  CL72 HEK-293T C144(1.21)  LDD1668  [18]
 LDCM0466  CL73 HEK-293T C144(0.92)  LDD1669  [18]
 LDCM0467  CL74 HEK-293T C144(1.10)  LDD1670  [18]
 LDCM0471  CL78 HEK-293T C144(1.29)  LDD1674  [18]
 LDCM0472  CL79 HEK-293T C144(1.00)  LDD1675  [18]
 LDCM0473  CL8 HEK-293T C144(0.96)  LDD1676  [18]
 LDCM0474  CL80 HEK-293T C144(1.25)  LDD1677  [18]
 LDCM0475  CL81 HEK-293T C144(1.12)  LDD1678  [18]
 LDCM0476  CL82 HEK-293T C144(1.01)  LDD1679  [18]
 LDCM0477  CL83 HEK-293T C144(1.11)  LDD1680  [18]
 LDCM0478  CL84 HEK-293T C144(1.23)  LDD1681  [18]
 LDCM0479  CL85 HEK-293T C144(1.05)  LDD1682  [18]
 LDCM0480  CL86 HEK-293T C144(0.97)  LDD1683  [18]
 LDCM0484  CL9 HEK-293T C144(1.28)  LDD1687  [18]
 LDCM0485  CL90 HEK-293T C144(0.68)  LDD1688  [18]
 LDCM0486  CL91 HEK-293T C144(1.01)  LDD1689  [18]
 LDCM0487  CL92 HEK-293T C144(1.23)  LDD1690  [18]
 LDCM0488  CL93 HEK-293T C144(0.95)  LDD1691  [18]
 LDCM0489  CL94 HEK-293T C144(0.79)  LDD1692  [18]
 LDCM0490  CL95 HEK-293T C144(0.98)  LDD1693  [18]
 LDCM0491  CL96 HEK-293T C144(1.22)  LDD1694  [18]
 LDCM0492  CL97 HEK-293T C144(0.74)  LDD1695  [18]
 LDCM0493  CL98 HEK-293T C144(1.03)  LDD1696  [18]
 LDCM0427  Fragment51 HEK-293T C144(1.10)  LDD1631  [18]
 LDCM0116  HHS-0101 DM93 Y24(0.93)  LDD0264  [5]
 LDCM0117  HHS-0201 DM93 Y24(0.84)  LDD0265  [5]
 LDCM0118  HHS-0301 DM93 Y24(0.82)  LDD0266  [5]
 LDCM0119  HHS-0401 DM93 Y24(0.85)  LDD0267  [5]
 LDCM0120  HHS-0701 DM93 Y24(0.75)  LDD0268  [5]
 LDCM0107  IAA HeLa N.A.  LDD0221  [14]
 LDCM0123  JWB131 DM93 Y24(1.00)  LDD0285  [4]
 LDCM0124  JWB142 DM93 Y24(1.41)  LDD0286  [4]
 LDCM0125  JWB146 DM93 Y24(1.16)  LDD0287  [4]
 LDCM0126  JWB150 DM93 Y24(1.58)  LDD0288  [4]
 LDCM0127  JWB152 DM93 Y24(2.41)  LDD0289  [4]
 LDCM0128  JWB198 DM93 Y24(0.24)  LDD0290  [4]
 LDCM0129  JWB202 DM93 Y24(1.03)  LDD0291  [4]
 LDCM0130  JWB211 DM93 Y24(1.08)  LDD0292  [4]
 LDCM0022  KB02 HEK-293T C144(1.04)  LDD1492  [18]
 LDCM0023  KB03 HEK-293T C144(1.04)  LDD1497  [18]
 LDCM0024  KB05 HEK-293T C144(0.95)  LDD1502  [18]
 LDCM0109  NEM HeLa H89(0.00); H87(0.00)  LDD0223  [14]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 6 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Histone-arginine methyltransferase CARM1 (CARM1) Protein arginine N-methyltransferase family Q86X55
Protein N-terminal glutamine amidohydrolase (NTAQ1) NTAQ1 family Q96HA8
Cleavage and polyadenylation specificity factor subunit 5 (NUDT21) Nudix hydrolase family O43809
Ubiquitin thioesterase ZRANB1 (ZRANB1) Peptidase C64 family Q9UGI0
E3 ubiquitin-protein ligase TRIM23 (TRIM23) Arf family P36406
Zinc finger protein RFP (TRIM27) TRIM/RBCC family P14373
Transporter and channel
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
AP-4 complex accessory subunit Tepsin (TEPSIN) . Q96N21
Transcription factor
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Zinc finger protein 655 (ZNF655) Krueppel C2H2-type zinc-finger protein family Q8N720
Other
Click To Hide/Show 9 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Ataxin-1 (ATXN1) ATXN1 family P54253
Protein FAM90A1 (FAM90A1) FAM90 family Q86YD7
Golgin subfamily A member 2 (GOLGA2) GOLGA2 family Q08379
Glial fibrillary acidic protein (GFAP) Intermediate filament family P14136
Keratin, type II cytoskeletal 75 (KRT75) Intermediate filament family O95678
Cleavage and polyadenylation specificity factor subunit 6 (CPSF6) RRM CPSF6/7 family Q16630
Cleavage and polyadenylation specificity factor subunit 7 (CPSF7) RRM CPSF6/7 family Q8N684
Myosin light polypeptide 6 (MYL6) . P60660
S100P-binding protein (S100PBP) . Q96BU1

References

1 Chemoproteomic profiling of kinases in live cells using electrophilic sulfonyl triazole probes. Chem Sci. 2021 Jan 21;12(9):3295-3307. doi: 10.1039/d0sc06623k.
2 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
3 An Azo Coupling-Based Chemoproteomic Approach to Systematically Profile the Tyrosine Reactivity in the Human Proteome. Anal Chem. 2021 Jul 27;93(29):10334-10342. doi: 10.1021/acs.analchem.1c01935. Epub 2021 Jul 12.
4 Chemoproteomic profiling of kinases in live cells using electrophilic sulfonyl triazole probes. Chem Sci. 2021 Jan 21;12(9):3295-3307. doi: 10.1039/d0sc06623k.
5 Discovery of a Cell-Active SuTEx Ligand of Prostaglandin Reductase 2. Chembiochem. 2021 Jun 15;22(12):2134-2139. doi: 10.1002/cbic.202000879. Epub 2021 Apr 29.
6 Global targeting of functional tyrosines using sulfur-triazole exchange chemistry. Nat Chem Biol. 2020 Feb;16(2):150-159. doi: 10.1038/s41589-019-0404-5. Epub 2019 Nov 25.
7 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
8 Targeted Proteomic Approaches for Proteome-Wide Characterizations of the AMP-Binding Capacities of Kinases. J Proteome Res. 2022 Aug 5;21(8):2063-2070. doi: 10.1021/acs.jproteome.2c00225. Epub 2022 Jul 12.
9 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
10 SP3-Enabled Rapid and High Coverage Chemoproteomic Identification of Cell-State-Dependent Redox-Sensitive Cysteines. Mol Cell Proteomics. 2022 Apr;21(4):100218. doi: 10.1016/j.mcpro.2022.100218. Epub 2022 Feb 25.
Mass spectrometry data entry: PXD029500 , PXD031647
11 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
12 Solid Phase Synthesis of Fluorosulfate Containing Macrocycles for Chemoproteomic Workflows. bioRxiv [Preprint]. 2023 Feb 18:2023.02.17.529022. doi: 10.1101/2023.02.17.529022.
Mass spectrometry data entry: PXD039931
13 Tunable Amine-Reactive Electrophiles for Selective Profiling of Lysine. Angew Chem Int Ed Engl. 2022 Jan 26;61(5):e202112107. doi: 10.1002/anie.202112107. Epub 2021 Dec 16.
14 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
15 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
16 Differently Tagged Probes for Protein Profiling of Mitochondria. Chembiochem. 2019 May 2;20(9):1155-1160. doi: 10.1002/cbic.201800735. Epub 2019 Mar 26.
17 Ligand and Target Discovery by Fragment-Based Screening in Human Cells. Cell. 2017 Jan 26;168(3):527-541.e29. doi: 10.1016/j.cell.2016.12.029. Epub 2017 Jan 19.
18 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402