Details of the Target

General Information of Target

Target ID LDTP00383
Target Name AP-3 complex subunit delta-1 (AP3D1)
Gene Name AP3D1
Gene ID 8943
Synonyms
AP-3 complex subunit delta-1; AP-3 complex subunit delta; Adaptor-related protein complex 3 subunit delta-1; Delta-adaptin
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MALKMVKGSIDRMFDKNLQDLVRGIRNHKEDEAKYISQCIDEIKQELKQDNIAVKANAVC
KLTYLQMLGYDISWAAFNIIEVMSASKFTFKRIGYLAASQSFHEGTDVIMLTTNQIRKDL
SSPSQYDTGVALTGLSCFVTPDLARDLANDIMTLMSHTKPYIRKKAVLIMYKVFLKYPES
LRPAFPRLKEKLEDPDPGVQSAAVNVICELARRNPKNYLSLAPLFFKLMTSSTNNWVLIK
IIKLFGALTPLEPRLGKKLIEPLTNLIHSTSAMSLLYECVNTVIAVLISLSSGMPNHSAS
IQLCVQKLRILIEDSDQNLKYLGLLAMSKILKTHPKSVQSHKDLILQCLDDKDESIRLRA
LDLLYGMVSKKNLMEIVKKLMTHVDKAEGTTYRDELLTKIIDICSQSNYQYITNFEWYIS
ILVELTRLEGTRHGHLIAAQMLDVAIRVKAIRKFAVSQMSALLDSAHLLASSTQRNGICE
VLYAAAWICGEFSEHLQEPHHTLEAMLRPRVTTLPGHIQAVYVQNVVKLYASILQQKEQA
GEAEGAQAVTQLMVDRLPQFVQSADLEVQERASCILQLVKHIQKLQAKDVPVAEEVSALF
AGELNPVAPKAQKKVPVPEGLDLDAWINEPLSDSESEDERPRAVFHEEEQRRPKHRPSEA
DEEELARRREARKQEQANNPFYIKSSPSPQKRYQDTPGVEHIPVVQIDLSVPLKVPGLPM
SDQYVKLEEERRHRQKLEKDKRRKKRKEKEKKGKRRHSSLPTESDEDIAPAQQVDIVTEE
MPENALPSDEDDKDPNDPYRALDIDLDKPLADSEKLPIQKHRNTETSKSPEKDVPMVEKK
SKKPKKKEKKHKEKERDKEKKKEKEKKKSPKPKKKKHRKEKEERTKGKKKSKKQPPGSEE
AAGEPVQNGAPEEEQLPPESSYSLLAENSYVKMTCDIRGSLQEDSQVTVAIVLENRSSSI
LKGMELSVLDSLNARMARPQGSSVHDGVPVPFQLPPGVSNEAQYVFTIQSIVMAQKLKGT
LSFIAKNDEGATHEKLDFRLHFSCSSYLITTPCYSDAFAKLLESGDLSMSSIKVDGIRMS
FQNLLAKICFHHHFSVVERVDSCASMYSRSIQGHHVCLLVKKGENSVSVDGKCSDSTLLS
NLLEEMKATLAKC
Target Bioclass
Transporter and channel
Family
Adaptor complexes large subunit family
Subcellular location
Cytoplasm
Function
Part of the AP-3 complex, an adaptor-related complex which is not clathrin-associated. The complex is associated with the Golgi region as well as more peripheral structures. It facilitates the budding of vesicles from the Golgi membrane and may be directly involved in trafficking to lysosomes. Involved in process of CD8+ T-cell and NK cell degranulation. In concert with the BLOC-1 complex, AP-3 is required to target cargos into vesicles assembled at cell bodies for delivery into neurites and nerve terminals.
Uniprot ID
O14617
Ensemble ID
ENST00000345016.9
HGNC ID
HGNC:568

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 24 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
m-APA
 Probe Info  		10.88  LDD0402  [1]
A-EBA
 Probe Info  		3.15  LDD0215  [2]
STPyne
 Probe Info  		K580(8.71); K584(9.70); K7(8.25); K820(1.15)  LDD0277  [3]
DBIA
 Probe Info  		C60(4.35); C574(7.55); C997(1.91); C971(2.09)  LDD3311  [4]
BTD
 Probe Info  		C60(1.52); C1153(1.38)  LDD1700  [5]
AHL-Pu-1
 Probe Info  		C574(2.53)  LDD0168  [6]
HHS-465
 Probe Info  		Y682(1.88)  LDD2237  [7]
ATP probe
 Probe Info  		N.A.  LDD0199  [8]
4-Iodoacetamidophenylacetylene
 Probe Info  		C574(0.00); C348(0.00); C208(0.00); C1117(0.00)  LDD0038  [9]
IA-alkyne
 Probe Info  		C574(0.00); C348(0.00); C1133(0.00)  LDD0032  [10]
IPIAA_L
 Probe Info  		C1133(0.00); C39(0.00)  LDD0031  [11]
Lodoacetamide azide
 Probe Info  		C574(0.00); C1133(0.00); C348(0.00); C208(0.00)  LDD0037  [9]
NAIA_4
 Probe Info  		N.A.  LDD2226  [12]
TFBX
 Probe Info  		C1103(0.00); C574(0.00)  LDD0027  [13]
WYneN
 Probe Info  		N.A.  LDD0021  [14]
Compound 10
 Probe Info  		C1103(0.00); C574(0.00)  LDD2216  [15]
IPM
 Probe Info  		C1103(0.00); C574(0.00)  LDD0005  [14]
VSF
 Probe Info  		N.A.  LDD0007  [14]
1c-yne
 Probe Info  		K588(0.00); K610(0.00)  LDD0228  [16]
Acrolein
 Probe Info  		C348(0.00); C208(0.00); C574(0.00); C935(0.00)  LDD0217  [17]
Methacrolein
 Probe Info  		C574(0.00); C348(0.00)  LDD0218  [17]
W1
 Probe Info  		C39(0.00); C1103(0.00); C348(0.00)  LDD0236  [18]
AOyne
 Probe Info  		9.30  LDD0443  [19]
NAIA_5
 Probe Info  		C574(0.00); C1133(0.00); C1089(0.00); C208(0.00)  LDD2223  [12]
PAL-AfBPP Probe
Click To Hide/Show 8 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C095
 Probe Info  		5.54  LDD1786  [20]
C183
 Probe Info  		9.58  LDD1861  [20]
C196
 Probe Info  		13.55  LDD1872  [20]
C243
 Probe Info  		12.21  LDD1916  [20]
C349
 Probe Info  		8.94  LDD2010  [20]
FFF probe4
 Probe Info  		12.65  LDD0466  [21]
STS-2
 Probe Info  		N.A.  LDD0138  [22]
OEA-DA
 Probe Info  		3.07  LDD0046  [23]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0548  1-(4-(Benzo[d][1,3]dioxol-5-ylmethyl)piperazin-1-yl)-2-nitroethan-1-one MDA-MB-231 C574(0.36)  LDD2142  [5]
 LDCM0502  1-(Cyanoacetyl)piperidine MDA-MB-231 C60(0.63)  LDD2095  [5]
 LDCM0537  2-Cyano-N,N-dimethylacetamide MDA-MB-231 C60(0.73)  LDD2130  [5]
 LDCM0524  2-Cyano-N-(2-morpholin-4-yl-ethyl)-acetamide MDA-MB-231 C60(0.99); C1153(1.06)  LDD2117  [5]
 LDCM0558  2-Cyano-N-phenylacetamide MDA-MB-231 C60(1.12); C1153(0.81)  LDD2152  [5]
 LDCM0539  3-(4-Isopropylpiperazin-1-yl)-3-oxopropanenitrile MDA-MB-231 C60(1.09)  LDD2132  [5]
 LDCM0025  4SU-RNA HEK-293T C574(2.53)  LDD0168  [6]
 LDCM0026  4SU-RNA+native RNA HEK-293T C574(8.42); C1103(2.51)  LDD0169  [6]
 LDCM0545  Acetamide MDA-MB-231 C574(0.49)  LDD2138  [5]
 LDCM0520  AKOS000195272 MDA-MB-231 C60(1.15)  LDD2113  [5]
 LDCM0156  Aniline NCI-H1299 12.12  LDD0403  [1]
 LDCM0498  BS-3668 MDA-MB-231 C574(0.53)  LDD2091  [5]
 LDCM0108  Chloroacetamide HeLa C574(0.00); C348(0.00); H655(0.00)  LDD0222  [17]
 LDCM0632  CL-Sc Hep-G2 C574(1.23); C1103(0.59)  LDD2227  [12]
 LDCM0634  CY-0357 Hep-G2 C1103(0.52)  LDD2228  [12]
 LDCM0213  Electrophilic fragment 2 MDA-MB-231 C574(7.17); C60(0.89)  LDD1702  [5]
 LDCM0625  F8 Ramos C1103(1.03); C574(0.65)  LDD2187  [24]
 LDCM0572  Fragment10 Ramos C1103(1.47); C574(0.86)  LDD2189  [24]
 LDCM0573  Fragment11 Ramos C1103(0.79); C574(20.00)  LDD2190  [24]
 LDCM0574  Fragment12 Ramos C1103(0.95); C574(0.83)  LDD2191  [24]
 LDCM0575  Fragment13 Ramos C1103(1.05); C574(0.93)  LDD2192  [24]
 LDCM0576  Fragment14 Ramos C1103(1.00); C574(1.94)  LDD2193  [24]
 LDCM0579  Fragment20 Ramos C1103(0.84); C574(0.64)  LDD2194  [24]
 LDCM0580  Fragment21 Ramos C1103(1.10); C574(0.99)  LDD2195  [24]
 LDCM0582  Fragment23 Ramos C1103(0.91); C574(0.39)  LDD2196  [24]
 LDCM0578  Fragment27 Ramos C1103(1.02); C574(0.97)  LDD2197  [24]
 LDCM0586  Fragment28 Ramos C1103(0.97); C574(1.88)  LDD2198  [24]
 LDCM0588  Fragment30 Ramos C1103(1.79); C574(1.06)  LDD2199  [24]
 LDCM0589  Fragment31 Ramos C1103(1.19)  LDD2200  [24]
 LDCM0590  Fragment32 Ramos C1103(1.08); C574(0.78)  LDD2201  [24]
 LDCM0468  Fragment33 Ramos C1103(1.08); C574(1.05)  LDD2202  [24]
 LDCM0596  Fragment38 Ramos C1103(0.93); C574(0.88)  LDD2203  [24]
 LDCM0566  Fragment4 Ramos C1103(1.14); C574(0.87)  LDD2184  [24]
 LDCM0610  Fragment52 Ramos C1103(1.34); C574(2.12)  LDD2204  [24]
 LDCM0614  Fragment56 Ramos C1103(1.52); C574(1.47)  LDD2205  [24]
 LDCM0569  Fragment7 Ramos C1103(0.92); C574(0.71)  LDD2186  [24]
 LDCM0571  Fragment9 Ramos C1103(1.14); C574(0.81)  LDD2188  [24]
 LDCM0107  IAA HeLa N.A.  LDD0221  [17]
 LDCM0022  KB02 Ramos C1103(1.19); C574(0.72)  LDD2182  [24]
 LDCM0023  KB03 MDA-MB-231 C574(19.65); C60(0.81)  LDD1701  [5]
 LDCM0024  KB05 G361 C60(4.35); C574(7.55); C997(1.91); C971(2.09)  LDD3311  [4]
 LDCM0528  N-(4-bromophenyl)-2-cyano-N-phenylacetamide MDA-MB-231 C60(0.85); C574(0.86)  LDD2121  [5]
 LDCM0109  NEM HeLa N.A.  LDD0223  [17]
 LDCM0496  Nucleophilic fragment 11a MDA-MB-231 C60(0.52); C574(0.81)  LDD2089  [5]
 LDCM0500  Nucleophilic fragment 13a MDA-MB-231 C60(0.92); C1153(1.01); C39(1.45)  LDD2093  [5]
 LDCM0504  Nucleophilic fragment 15a MDA-MB-231 C60(1.01)  LDD2097  [5]
 LDCM0506  Nucleophilic fragment 16a MDA-MB-231 C60(1.00); C1153(1.02)  LDD2099  [5]
 LDCM0508  Nucleophilic fragment 17a MDA-MB-231 C60(1.14)  LDD2101  [5]
 LDCM0514  Nucleophilic fragment 20a MDA-MB-231 C60(1.01); C1153(0.99)  LDD2107  [5]
 LDCM0516  Nucleophilic fragment 21a MDA-MB-231 C1153(0.53); C935(1.33)  LDD2109  [5]
 LDCM0518  Nucleophilic fragment 22a MDA-MB-231 C60(1.07)  LDD2111  [5]
 LDCM0521  Nucleophilic fragment 23b MDA-MB-231 C574(0.89)  LDD2114  [5]
 LDCM0526  Nucleophilic fragment 26a MDA-MB-231 C60(2.64)  LDD2119  [5]
 LDCM0527  Nucleophilic fragment 26b MDA-MB-231 C60(0.94); C1153(0.89)  LDD2120  [5]
 LDCM0530  Nucleophilic fragment 28a MDA-MB-231 C60(0.84); C1153(0.75)  LDD2123  [5]
 LDCM0532  Nucleophilic fragment 29a MDA-MB-231 C60(0.98); C1103(0.88)  LDD2125  [5]
 LDCM0534  Nucleophilic fragment 30a MDA-MB-231 C60(1.01); C1153(1.12)  LDD2127  [5]
 LDCM0535  Nucleophilic fragment 30b MDA-MB-231 C60(2.40); C1153(0.88)  LDD2128  [5]
 LDCM0536  Nucleophilic fragment 31 MDA-MB-231 C60(1.05); C1153(0.88)  LDD2129  [5]
 LDCM0541  Nucleophilic fragment 36 MDA-MB-231 C60(0.59)  LDD2134  [5]
 LDCM0542  Nucleophilic fragment 37 MDA-MB-231 C1153(1.26); C39(2.18)  LDD2135  [5]
 LDCM0543  Nucleophilic fragment 38 MDA-MB-231 C60(1.06)  LDD2136  [5]
 LDCM0544  Nucleophilic fragment 39 MDA-MB-231 C60(1.06); C1153(0.88)  LDD2137  [5]
 LDCM0211  Nucleophilic fragment 3b MDA-MB-231 C60(1.52); C1153(1.38)  LDD1700  [5]
 LDCM0546  Nucleophilic fragment 40 MDA-MB-231 C60(0.65); C1153(0.47)  LDD2140  [5]
 LDCM0547  Nucleophilic fragment 41 MDA-MB-231 C574(0.63)  LDD2141  [5]
 LDCM0549  Nucleophilic fragment 43 MDA-MB-231 C60(1.26); C1153(0.90)  LDD2143  [5]
 LDCM0550  Nucleophilic fragment 5a MDA-MB-231 C60(2.74)  LDD2144  [5]
 LDCM0552  Nucleophilic fragment 6a MDA-MB-231 C60(0.89); C1153(0.99)  LDD2146  [5]
 LDCM0553  Nucleophilic fragment 6b MDA-MB-231 C1153(2.78)  LDD2147  [5]
 LDCM0554  Nucleophilic fragment 7a MDA-MB-231 C60(0.64)  LDD2148  [5]
 LDCM0627  NUDT7-COV-1 HEK-293T C574(0.07)  LDD2206  [25]
 LDCM0628  OTUB2-COV-1 HEK-293T C574(1.13)  LDD2207  [25]

References

1 Quantitative and Site-Specific Chemoproteomic Profiling of Targets of Acrolein. Chem Res Toxicol. 2019 Mar 18;32(3):467-473. doi: 10.1021/acs.chemrestox.8b00343. Epub 2019 Jan 15.
2 2-Ethynylbenzaldehyde-Based, Lysine-Targeting Irreversible Covalent Inhibitors for Protein Kinases and Nonkinases. J Am Chem Soc. 2023 Feb 12. doi: 10.1021/jacs.2c11595. Online ahead of print.
Mass spectrometry data entry: PXD037665
3 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
4 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
5 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
6 Chemoproteomic capture of RNA binding activity in living cells. Nat Commun. 2023 Oct 7;14(1):6282. doi: 10.1038/s41467-023-41844-z.
Mass spectrometry data entry: PXD044625
7 Global targeting of functional tyrosines using sulfur-triazole exchange chemistry. Nat Chem Biol. 2020 Feb;16(2):150-159. doi: 10.1038/s41589-019-0404-5. Epub 2019 Nov 25.
8 Targeted Proteomic Approaches for Proteome-Wide Characterizations of the AMP-Binding Capacities of Kinases. J Proteome Res. 2022 Aug 5;21(8):2063-2070. doi: 10.1021/acs.jproteome.2c00225. Epub 2022 Jul 12.
9 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
10 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
11 SP3-Enabled Rapid and High Coverage Chemoproteomic Identification of Cell-State-Dependent Redox-Sensitive Cysteines. Mol Cell Proteomics. 2022 Apr;21(4):100218. doi: 10.1016/j.mcpro.2022.100218. Epub 2022 Feb 25.
Mass spectrometry data entry: PXD029500 , PXD031647
12 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
13 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
14 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
15 Multiplexed CuAAC Suzuki-Miyaura Labeling for Tandem Activity-Based Chemoproteomic Profiling. Anal Chem. 2021 Feb 2;93(4):2610-2618. doi: 10.1021/acs.analchem.0c04726. Epub 2021 Jan 20.
Mass spectrometry data entry: PXD022279
16 Tunable Amine-Reactive Electrophiles for Selective Profiling of Lysine. Angew Chem Int Ed Engl. 2022 Jan 26;61(5):e202112107. doi: 10.1002/anie.202112107. Epub 2021 Dec 16.
17 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
18 Oxidant-Induced Bioconjugation for Protein Labeling in Live Cells. ACS Chem Biol. 2023 Jan 20;18(1):112-122. doi: 10.1021/acschembio.2c00740. Epub 2022 Dec 21.
19 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
20 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587
21 Ligand and Target Discovery by Fragment-Based Screening in Human Cells. Cell. 2017 Jan 26;168(3):527-541.e29. doi: 10.1016/j.cell.2016.12.029. Epub 2017 Jan 19.
22 Design and synthesis of minimalist terminal alkyne-containing diazirine photo-crosslinkers and their incorporation into kinase inhibitors for cell- and tissue-based proteome profiling. Angew Chem Int Ed Engl. 2013 Aug 12;52(33):8551-6. doi: 10.1002/anie.201300683. Epub 2013 Jun 10.
23 Mapping Protein Targets of Bioactive Small Molecules Using Lipid-Based Chemical Proteomics. ACS Chem Biol. 2017 Oct 20;12(10):2671-2681. doi: 10.1021/acschembio.7b00581. Epub 2017 Sep 20.
Mass spectrometry data entry: PXD007570
24 Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry. Methods Enzymol. 2023;679:295-322. doi: 10.1016/bs.mie.2022.07.037. Epub 2022 Sep 7.
Mass spectrometry data entry: PXD027578
25 Rapid Covalent-Probe Discovery by Electrophile-Fragment Screening. J Am Chem Soc. 2019 Jun 5;141(22):8951-8968. doi: 10.1021/jacs.9b02822. Epub 2019 May 22.