Details of the Target

General Information of Target

Target ID LDTP14136
Target Name Mitochondrial import inner membrane translocase subunit Tim13 (TIMM13)
Gene Name TIMM13
Gene ID 26517
Synonyms
TIM13B; TIMM13A; TIMM13B; Mitochondrial import inner membrane translocase subunit Tim13
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MAGKVRSLLPPLLLAAAGLAGLLLLCVPTRDVREPPALKYGIVLDAGSSHTSMFIYKWPA
DKENDTGIVGQHSSCDVPGGGISSYADNPSGASQSLVGCLEQALQDVPKERHAGTPLYLG
ATAGMRLLNLTNPEASTSVLMAVTHTLTQYPFDFRGARILSGQEEGVFGWVTANYLLENF
IKYGWVGRWFRPRKGTLGAMDLGGASTQITFETTSPAEDRASEVQLHLYGQHYRVYTHSF
LCYGRDQVLQRLLASALQTHGFHPCWPRGFSTQVLLGDVYQSPCTMAQRPQNFNSSARVS
LSGSSDPHLCRDLVSGLFSFSSCPFSRCSFNGVFQPPVAGNFVAFSAFFYTVDFLRTSMG
LPVATLQQLEAAAVNVCNQTWAQLQARVPGQRARLADYCAGAMFVQQLLSRGYGFDERAF
GGVIFQKKAADTAVGWALGYMLNLTNLIPADPPGLRKGTDFSSWVVLLLLFASALLAALV
LLLRQVHSAKLPSTI
Target Bioclass
Other
Family
Small Tim family
Subcellular location
Mitochondrion inner membrane
Function
Mitochondrial intermembrane chaperone that participates in the import and insertion of some multi-pass transmembrane proteins into the mitochondrial inner membrane. Also required for the transfer of beta-barrel precursors from the TOM complex to the sorting and assembly machinery (SAM complex) of the outer membrane. Acts as a chaperone-like protein that protects the hydrophobic precursors from aggregation and guide them through the mitochondrial intermembrane space. The TIMM8-TIMM13 complex mediates the import of proteins such as TIMM23, SLC25A12/ARALAR1 and SLC25A13/ARALAR2, while the predominant TIMM9-TIMM10 70 kDa complex mediates the import of much more proteins.
Uniprot ID
Q9Y5L4
Ensemble ID
ENST00000215570.8
HGNC ID
HGNC:11816

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 17 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C-Sul
 Probe Info  		4.15  LDD0066  [1]
STPyne
 Probe Info  		K27(20.00)  LDD2217  [2]
AZ-9
 Probe Info  		D8(10.00)  LDD2209  [3]
BTD
 Probe Info  		C50(0.77); C46(0.64)  LDD2097  [4]
DBIA
 Probe Info  		C65(1.37); C69(1.37)  LDD2171  [5]
ATP probe
 Probe Info  		K49(0.00); K53(0.00)  LDD0199  [6]
4-Iodoacetamidophenylacetylene
 Probe Info  		N.A.  LDD0038  [7]
IA-alkyne
 Probe Info  		N.A.  LDD0162  [8]
Lodoacetamide azide
 Probe Info  		N.A.  LDD0037  [7]
JW-RF-010
 Probe Info  		N.A.  LDD0026  [9]
TFBX
 Probe Info  		N.A.  LDD0027  [9]
WYneC
 Probe Info  		N.A.  LDD0014  [10]
ENE
 Probe Info  		N.A.  LDD0006  [10]
IPM
 Probe Info  		N.A.  LDD2156  [11]
NPM
 Probe Info  		N.A.  LDD0016  [10]
1c-yne
 Probe Info  		N.A.  LDD0228  [12]
NAIA_5
 Probe Info  		C50(0.00); C46(0.00)  LDD2223  [13]
PAL-AfBPP Probe
Click To Hide/Show 6 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C004
 Probe Info  		8.06  LDD1714  [14]
C106
 Probe Info  		16.80  LDD1793  [14]
C218
 Probe Info  		11.39  LDD1892  [14]
C349
 Probe Info  		11.96  LDD2010  [14]
C350
 Probe Info  		24.25  LDD2011  [14]
FFF probe11
 Probe Info  		17.14  LDD0471  [15]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0520  AKOS000195272 MDA-MB-231 C50(0.58)  LDD2113  [4]
 LDCM0020  ARS-1620 HCC44 C65(1.37); C69(1.37)  LDD2171  [5]
 LDCM0504  Nucleophilic fragment 15a MDA-MB-231 C50(0.77); C46(0.64)  LDD2097  [4]
 LDCM0505  Nucleophilic fragment 15b MDA-MB-231 C46(1.59)  LDD2098  [4]
 LDCM0507  Nucleophilic fragment 16b MDA-MB-231 C50(0.26)  LDD2100  [4]
 LDCM0508  Nucleophilic fragment 17a MDA-MB-231 C50(1.10); C46(0.65)  LDD2101  [4]
 LDCM0511  Nucleophilic fragment 18b MDA-MB-231 C50(0.34)  LDD2104  [4]
 LDCM0522  Nucleophilic fragment 24a MDA-MB-231 C50(0.39); C46(0.87)  LDD2115  [4]
 LDCM0541  Nucleophilic fragment 36 MDA-MB-231 C50(0.72); C46(4.37)  LDD2134  [4]
 LDCM0553  Nucleophilic fragment 6b MDA-MB-231 C50(0.47)  LDD2147  [4]
 LDCM0554  Nucleophilic fragment 7a MDA-MB-231 C50(0.75)  LDD2148  [4]
 LDCM0021  THZ1 HCT 116 C65(1.37); C69(1.37)  LDD2173  [5]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Other
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Harmonin-binding protein USHBP1 (USHBP1) MCC family Q8N6Y0
Mitochondrial import inner membrane translocase subunit Tim8 A (TIMM8A) Small Tim family O60220

References

1 Low-Toxicity Sulfonium-Based Probes for Cysteine-Specific Profiling in Live Cells. Anal Chem. 2022 Mar 15;94(10):4366-4372. doi: 10.1021/acs.analchem.1c05129. Epub 2022 Mar 4.
2 Global profiling of lysine reactivity and ligandability in the human proteome. Nat Chem. 2017 Dec;9(12):1181-1190. doi: 10.1038/nchem.2826. Epub 2017 Jul 31.
3 2H-Azirine-Based Reagents for Chemoselective Bioconjugation at Carboxyl Residues Inside Live Cells. J Am Chem Soc. 2020 Apr 1;142(13):6051-6059. doi: 10.1021/jacs.9b12116. Epub 2020 Mar 23.
4 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
5 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
6 Targeted Proteomic Approaches for Proteome-Wide Characterizations of the AMP-Binding Capacities of Kinases. J Proteome Res. 2022 Aug 5;21(8):2063-2070. doi: 10.1021/acs.jproteome.2c00225. Epub 2022 Jul 12.
7 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
8 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
9 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
10 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
11 Benchmarking Cleavable Biotin Tags for Peptide-Centric Chemoproteomics. J Proteome Res. 2022 May 6;21(5):1349-1358. doi: 10.1021/acs.jproteome.2c00174. Epub 2022 Apr 25.
Mass spectrometry data entry: PXD031019
12 Tunable Amine-Reactive Electrophiles for Selective Profiling of Lysine. Angew Chem Int Ed Engl. 2022 Jan 26;61(5):e202112107. doi: 10.1002/anie.202112107. Epub 2021 Dec 16.
13 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
14 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587
15 Ligand and Target Discovery by Fragment-Based Screening in Human Cells. Cell. 2017 Jan 26;168(3):527-541.e29. doi: 10.1016/j.cell.2016.12.029. Epub 2017 Jan 19.