Details of the Target

General Information of Target

Target ID LDTP13954
Target Name Complex I intermediate-associated protein 30, mitochondrial (NDUFAF1)
Gene Name NDUFAF1
Gene ID 51103
Synonyms
CIA30; Complex I intermediate-associated protein 30, mitochondrial; NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 1
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MNIMDFNVKKLAADAGTFLSRAVQFTEEKLGQAEKTELDAHLENLLSKAECTKIWTEKIM
KQTEVLLQPNPNARIEEFVYEKLDRKAPSRINNPELLGQYMIDAGTEFGPGTAYGNALIK
CGETQKRIGTADRELIQTSALNFLTPLRNFIEGDYKTIAKERKLLQNKRLDLDAAKTRLK
KAKAAETRNSSEQELRITQSEFDRQAEITRLLLEGISSTHAHHLRCLNDFVEAQMTYYAQ
CYQYMLDLQKQLGSFPSNYLSNNNQTSVTPVPSVLPNAIGSSAMASTSGLVITSPSNLSD
LKECSGSRKARVLYDYDAANSTELSLLADEVITVFSVVGMDSDWLMGERGNQKGKVPITY
LELLN
Target Bioclass
Other
Family
CIA30 family
Subcellular location
Mitochondrion
Function As part of the MCIA complex, involved in the assembly of the mitochondrial complex I.
Uniprot ID
Q9Y375
Ensemble ID
ENST00000260361.9
HGNC ID
HGNC:18828
ChEMBL ID
CHEMBL2363065

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 6 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
STPyne
 Probe Info  		K98(5.78)  LDD0277  [1]
DBIA
 Probe Info  		C183(1.25)  LDD0533  [2]
5E-2FA
 Probe Info  		N.A.  LDD2235  [3]
IA-alkyne
 Probe Info  		N.A.  LDD0036  [4]
Lodoacetamide azide
 Probe Info  		N.A.  LDD0037  [4]
Crotonaldehyde
 Probe Info  		N.A.  LDD0219  [5]
PAL-AfBPP Probe
Click To Hide/Show 6 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C091
 Probe Info  		11.31  LDD1782  [6]
C094
 Probe Info  		26.54  LDD1785  [6]
C246
 Probe Info  		17.51  LDD1919  [6]
C338
 Probe Info  		10.27  LDD2001  [6]
C355
 Probe Info  		28.44  LDD2016  [6]
C356
 Probe Info  		7.89  LDD2017  [6]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0216  AC100 HCT 116 C183(1.25)  LDD0533  [2]
 LDCM0217  AC101 HCT 116 C183(0.96)  LDD0534  [2]
 LDCM0218  AC102 HCT 116 C183(1.03)  LDD0535  [2]
 LDCM0219  AC103 HCT 116 C183(1.28)  LDD0536  [2]
 LDCM0220  AC104 HCT 116 C183(0.89)  LDD0537  [2]
 LDCM0221  AC105 HCT 116 C183(0.99)  LDD0538  [2]
 LDCM0222  AC106 HCT 116 C183(1.39)  LDD0539  [2]
 LDCM0223  AC107 HCT 116 C183(1.04)  LDD0540  [2]
 LDCM0224  AC108 HCT 116 C183(1.16)  LDD0541  [2]
 LDCM0225  AC109 HCT 116 C183(0.80)  LDD0542  [2]
 LDCM0227  AC110 HCT 116 C183(1.01)  LDD0544  [2]
 LDCM0228  AC111 HCT 116 C183(1.11)  LDD0545  [2]
 LDCM0229  AC112 HCT 116 C183(1.33)  LDD0546  [2]
 LDCM0230  AC113 HCT 116 C183(1.50)  LDD0547  [2]
 LDCM0231  AC114 HCT 116 C183(0.98)  LDD0548  [2]
 LDCM0232  AC115 HCT 116 C183(0.74)  LDD0549  [2]
 LDCM0233  AC116 HCT 116 C183(1.18)  LDD0550  [2]
 LDCM0234  AC117 HCT 116 C183(1.09)  LDD0551  [2]
 LDCM0235  AC118 HCT 116 C183(1.17)  LDD0552  [2]
 LDCM0236  AC119 HCT 116 C183(1.08)  LDD0553  [2]
 LDCM0238  AC120 HCT 116 C183(1.65)  LDD0555  [2]
 LDCM0239  AC121 HCT 116 C183(1.12)  LDD0556  [2]
 LDCM0240  AC122 HCT 116 C183(0.91)  LDD0557  [2]
 LDCM0241  AC123 HCT 116 C183(0.68)  LDD0558  [2]
 LDCM0242  AC124 HCT 116 C183(0.97)  LDD0559  [2]
 LDCM0243  AC125 HCT 116 C183(1.16)  LDD0560  [2]
 LDCM0244  AC126 HCT 116 C183(0.97)  LDD0561  [2]
 LDCM0245  AC127 HCT 116 C183(0.95)  LDD0562  [2]
 LDCM0285  AC25 HCT 116 C183(0.84)  LDD0602  [2]
 LDCM0286  AC26 HCT 116 C183(1.11)  LDD0603  [2]
 LDCM0287  AC27 HCT 116 C183(0.79)  LDD0604  [2]
 LDCM0288  AC28 HCT 116 C183(1.05)  LDD0605  [2]
 LDCM0289  AC29 HCT 116 C183(1.38)  LDD0606  [2]
 LDCM0291  AC30 HCT 116 C183(1.28)  LDD0608  [2]
 LDCM0292  AC31 HCT 116 C183(1.09)  LDD0609  [2]
 LDCM0293  AC32 HCT 116 C183(1.71)  LDD0610  [2]
 LDCM0294  AC33 HCT 116 C183(1.02)  LDD0611  [2]
 LDCM0295  AC34 HCT 116 C183(0.99)  LDD0612  [2]
 LDCM0308  AC46 HCT 116 C183(1.28)  LDD0625  [2]
 LDCM0309  AC47 HCT 116 C183(0.86)  LDD0626  [2]
 LDCM0310  AC48 HCT 116 C183(2.47)  LDD0627  [2]
 LDCM0311  AC49 HCT 116 C183(1.28)  LDD0628  [2]
 LDCM0313  AC50 HCT 116 C183(1.36)  LDD0630  [2]
 LDCM0314  AC51 HCT 116 C183(0.93)  LDD0631  [2]
 LDCM0315  AC52 HCT 116 C183(1.36)  LDD0632  [2]
 LDCM0316  AC53 HCT 116 C183(1.22)  LDD0633  [2]
 LDCM0317  AC54 HCT 116 C183(1.44)  LDD0634  [2]
 LDCM0318  AC55 HCT 116 C183(0.86)  LDD0635  [2]
 LDCM0319  AC56 HCT 116 C183(1.69)  LDD0636  [2]
 LDCM0365  AC98 HCT 116 C183(1.22)  LDD0682  [2]
 LDCM0366  AC99 HCT 116 C183(1.07)  LDD0683  [2]
 LDCM0382  CL112 HCT 116 C183(1.06)  LDD0699  [2]
 LDCM0383  CL113 HCT 116 C183(1.03)  LDD0700  [2]
 LDCM0384  CL114 HCT 116 C183(1.31)  LDD0701  [2]
 LDCM0385  CL115 HCT 116 C183(1.06)  LDD0702  [2]
 LDCM0386  CL116 HCT 116 C183(1.00)  LDD0703  [2]
 LDCM0392  CL121 HCT 116 C183(0.85)  LDD0709  [2]
 LDCM0393  CL122 HCT 116 C183(1.30)  LDD0710  [2]
 LDCM0394  CL123 HCT 116 C183(0.97)  LDD0711  [2]
 LDCM0395  CL124 HCT 116 C183(1.78)  LDD0712  [2]
 LDCM0625  F8 Ramos C183(0.80)  LDD2187  [7]
 LDCM0572  Fragment10 Ramos C183(2.32)  LDD2189  [7]
 LDCM0573  Fragment11 Ramos C183(2.19)  LDD2190  [7]
 LDCM0576  Fragment14 Ramos C183(0.57)  LDD2193  [7]
 LDCM0580  Fragment21 Ramos C183(0.94)  LDD2195  [7]
 LDCM0578  Fragment27 Ramos C183(0.82)  LDD2197  [7]
 LDCM0588  Fragment30 Ramos C183(0.78)  LDD2199  [7]
 LDCM0589  Fragment31 Ramos C183(0.99)  LDD2200  [7]
 LDCM0610  Fragment52 Ramos C183(0.90)  LDD2204  [7]
 LDCM0614  Fragment56 Ramos C183(1.01)  LDD2205  [7]
 LDCM0569  Fragment7 Ramos C183(0.45)  LDD2186  [7]
 LDCM0571  Fragment9 Ramos C183(1.54)  LDD2188  [7]
 LDCM0022  KB02 Ramos C183(0.36)  LDD2182  [7]
 LDCM0023  KB03 Ramos C183(0.45)  LDD2183  [7]
 LDCM0024  KB05 Ramos C183(0.33)  LDD2185  [7]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Inactive pancreatic lipase-related protein 1 (PNLIPRP1) Lipase family P54315
Lysoplasmalogenase TMEM86B (TMEM86B) TMEM86 family Q8N661
Transporter and channel
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Proton-coupled zinc antiporter SLC30A2 (SLC30A2) Cation diffusion facilitator (CDF) transporter (TC 2.A.4) family Q9BRI3
Sigma intracellular receptor 2 (TMEM97) TMEM97/sigma-2 receptor family Q5BJF2
Other
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Syntaxin-8 (STX8) Syntaxin family Q9UNK0
Protein YIPF6 (YIPF6) YIP1 family Q96EC8

References

1 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
2 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
3 Global profiling of functional histidines in live cells using small-molecule photosensitizer and chemical probe relay labelling. Nat Chem. 2024 Jun 4. doi: 10.1038/s41557-024-01545-6. Online ahead of print.
Mass spectrometry data entry: PXD042377
4 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
5 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
6 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587
7 Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry. Methods Enzymol. 2023;679:295-322. doi: 10.1016/bs.mie.2022.07.037. Epub 2022 Sep 7.
Mass spectrometry data entry: PXD027578