Details of the Target

General Information of Target

Target ID LDTP12382
Target Name Semaphorin-4B (SEMA4B)
Gene Name SEMA4B
Gene ID 10509
Synonyms
KIAA1745; SEMAC; Semaphorin-4B; Semaphorin-C
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MEPRAVAEAVETGEEDVIMEALRSYNQEHSQSFTFDDAQQEDRKRLAELLVSVLEQGLPP
SHRVIWLQSVRILSRDRNCLDPFTSRQSLQALACYADISVSEGSVPESADMDVVLESLKC
LCNLVLSSPVAQMLAAEARLVVKLTERVGLYRERSFPHDVQFFDLRLLFLLTALRTDVRQ
QLFQELKGVRLLTDTLELTLGVTPEGNPPPTLLPSQETERAMEILKVLFNITLDSIKGEV
DEEDAALYRHLGTLLRHCVMIATAGDRTEEFHGHAVNLLGNLPLKCLDVLLTLEPHGDST
EFMGVNMDVIRALLIFLEKRLHKTHRLKESVAPVLSVLTECARMHRPARKFLKAQVLPPL
RDVRTRPEVGEMLRNKLVRLMTHLDTDVKRVAAEFLFVLCSESVPRFIKYTGYGNAAGLL
AARGLMAGGRPEGQYSEDEDTDTDEYKEAKASINPVTGRVEEKPPNPMEGMTEEQKEHEA
MKLVTMFDKLSRNRVIQPMGMSPRGHLTSLQDAMCETMEQQLSSDPDSDPD
Target Bioclass
Other
Family
Semaphorin family
Subcellular location
Membrane
Function Inhibits axonal extension by providing local signals to specify territories inaccessible for growing axons.
Uniprot ID
Q9NPR2
Ensemble ID
ENST00000332496.10
HGNC ID
HGNC:10730

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
CAL148 SNV: p.I693M .
EFO21 SNV: p.E685D .
GB1 SNV: p.T159I .
HEC1 SNV: p.N607K .
HEC1B SNV: p.N607K .
HT SNV: p.V295E .
HT1197 SNV: p.C526Ter DBIA    Probe Info 
IPC298 SNV: p.E164G .
KMCH1 SNV: p.Y787F DBIA    Probe Info 
OE33 SNV: p.V207I .
SKMEL30 Substitution: p.A283V .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 5 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
BTD
 Probe Info  		C185(0.86)  LDD2092  [1]
IPM
 Probe Info  		C753(14.19); C821(3.75)  LDD1701  [1]
DBIA
 Probe Info  		C816(1.03)  LDD0532  [2]
IA-alkyne
 Probe Info  		N.A.  LDD0162  [3]
NAIA_5
 Probe Info  		C399(0.00); C131(0.00); C286(0.00)  LDD2223  [4]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0524  2-Cyano-N-(2-morpholin-4-yl-ethyl)-acetamide MDA-MB-231 C399(1.07)  LDD2117  [1]
 LDCM0539  3-(4-Isopropylpiperazin-1-yl)-3-oxopropanenitrile MDA-MB-231 C753(0.31)  LDD2132  [1]
 LDCM0214  AC1 HEK-293T C821(1.17)  LDD1507  [5]
 LDCM0215  AC10 HCT 116 C816(1.03)  LDD0532  [2]
 LDCM0216  AC100 HCT 116 C816(1.20)  LDD0533  [2]
 LDCM0217  AC101 HCT 116 C816(1.22)  LDD0534  [2]
 LDCM0218  AC102 HCT 116 C816(1.23)  LDD0535  [2]
 LDCM0219  AC103 HCT 116 C816(1.52)  LDD0536  [2]
 LDCM0220  AC104 HCT 116 C816(1.36)  LDD0537  [2]
 LDCM0221  AC105 HCT 116 C816(1.24)  LDD0538  [2]
 LDCM0222  AC106 HCT 116 C816(1.79)  LDD0539  [2]
 LDCM0223  AC107 HCT 116 C816(1.64)  LDD0540  [2]
 LDCM0224  AC108 HCT 116 C816(1.25)  LDD0541  [2]
 LDCM0225  AC109 HCT 116 C816(1.11)  LDD0542  [2]
 LDCM0226  AC11 HCT 116 C816(1.18)  LDD0543  [2]
 LDCM0227  AC110 HCT 116 C816(1.39)  LDD0544  [2]
 LDCM0228  AC111 HCT 116 C816(1.26)  LDD0545  [2]
 LDCM0229  AC112 HCT 116 C816(1.06)  LDD0546  [2]
 LDCM0230  AC113 HCT 116 C816(0.94)  LDD0547  [2]
 LDCM0231  AC114 HCT 116 C816(1.22)  LDD0548  [2]
 LDCM0232  AC115 HCT 116 C816(1.05)  LDD0549  [2]
 LDCM0233  AC116 HCT 116 C816(1.03)  LDD0550  [2]
 LDCM0234  AC117 HCT 116 C816(1.28)  LDD0551  [2]
 LDCM0235  AC118 HCT 116 C816(1.16)  LDD0552  [2]
 LDCM0236  AC119 HCT 116 C816(1.27)  LDD0553  [2]
 LDCM0237  AC12 HCT 116 C816(1.28)  LDD0554  [2]
 LDCM0238  AC120 HCT 116 C816(1.44)  LDD0555  [2]
 LDCM0239  AC121 HCT 116 C816(1.35)  LDD0556  [2]
 LDCM0240  AC122 HCT 116 C816(1.16)  LDD0557  [2]
 LDCM0241  AC123 HCT 116 C816(1.40)  LDD0558  [2]
 LDCM0242  AC124 HCT 116 C816(1.26)  LDD0559  [2]
 LDCM0243  AC125 HCT 116 C816(1.19)  LDD0560  [2]
 LDCM0244  AC126 HCT 116 C816(1.09)  LDD0561  [2]
 LDCM0245  AC127 HCT 116 C816(1.19)  LDD0562  [2]
 LDCM0246  AC128 HCT 116 C816(0.65)  LDD0563  [2]
 LDCM0247  AC129 HCT 116 C816(0.54)  LDD0564  [2]
 LDCM0249  AC130 HCT 116 C816(0.57)  LDD0566  [2]
 LDCM0250  AC131 HCT 116 C816(0.58)  LDD0567  [2]
 LDCM0251  AC132 HCT 116 C816(0.59)  LDD0568  [2]
 LDCM0252  AC133 HCT 116 C816(0.57)  LDD0569  [2]
 LDCM0253  AC134 HCT 116 C816(0.58)  LDD0570  [2]
 LDCM0254  AC135 HCT 116 C816(0.63)  LDD0571  [2]
 LDCM0255  AC136 HCT 116 C816(0.63)  LDD0572  [2]
 LDCM0256  AC137 HCT 116 C816(0.69)  LDD0573  [2]
 LDCM0257  AC138 HCT 116 C816(0.60)  LDD0574  [2]
 LDCM0258  AC139 HCT 116 C816(0.61)  LDD0575  [2]
 LDCM0259  AC14 HCT 116 C816(1.06)  LDD0576  [2]
 LDCM0260  AC140 HCT 116 C816(0.68)  LDD0577  [2]
 LDCM0261  AC141 HCT 116 C816(0.64)  LDD0578  [2]
 LDCM0262  AC142 HCT 116 C816(0.67)  LDD0579  [2]
 LDCM0263  AC143 HCT 116 C816(1.26)  LDD0580  [2]
 LDCM0264  AC144 HCT 116 C816(0.88)  LDD0581  [2]
 LDCM0265  AC145 HCT 116 C816(1.26)  LDD0582  [2]
 LDCM0266  AC146 HCT 116 C816(1.24)  LDD0583  [2]
 LDCM0267  AC147 HCT 116 C816(1.04)  LDD0584  [2]
 LDCM0268  AC148 HCT 116 C816(1.82)  LDD0585  [2]
 LDCM0269  AC149 HCT 116 C816(1.74)  LDD0586  [2]
 LDCM0270  AC15 HCT 116 C816(0.82)  LDD0587  [2]
 LDCM0271  AC150 HCT 116 C816(1.04)  LDD0588  [2]
 LDCM0272  AC151 HCT 116 C816(0.91)  LDD0589  [2]
 LDCM0273  AC152 HCT 116 C816(1.06)  LDD0590  [2]
 LDCM0274  AC153 HCT 116 C816(2.12)  LDD0591  [2]
 LDCM0621  AC154 HCT 116 C816(1.08)  LDD2158  [2]
 LDCM0622  AC155 HCT 116 C816(1.27)  LDD2159  [2]
 LDCM0623  AC156 HCT 116 C816(1.05)  LDD2160  [2]
 LDCM0624  AC157 HCT 116 C816(1.10)  LDD2161  [2]
 LDCM0276  AC17 HEK-293T C821(0.94)  LDD1515  [5]
 LDCM0277  AC18 HEK-293T C821(1.20)  LDD1516  [5]
 LDCM0278  AC19 HEK-293T C821(1.12)  LDD1517  [5]
 LDCM0279  AC2 HEK-293T C821(1.18)  LDD1518  [5]
 LDCM0285  AC25 HEK-293T C821(1.02)  LDD1524  [5]
 LDCM0286  AC26 HEK-293T C821(1.24)  LDD1525  [5]
 LDCM0287  AC27 HEK-293T C821(0.93)  LDD1526  [5]
 LDCM0290  AC3 HEK-293T C821(1.03)  LDD1529  [5]
 LDCM0294  AC33 HEK-293T C821(0.95)  LDD1533  [5]
 LDCM0295  AC34 HEK-293T C821(1.38)  LDD1534  [5]
 LDCM0296  AC35 HEK-293T C821(0.95)  LDD1535  [5]
 LDCM0303  AC41 HEK-293T C821(0.92)  LDD1542  [5]
 LDCM0304  AC42 HEK-293T C821(1.36)  LDD1543  [5]
 LDCM0305  AC43 HEK-293T C821(1.03)  LDD1544  [5]
 LDCM0311  AC49 HEK-293T C821(0.93)  LDD1550  [5]
 LDCM0313  AC50 HEK-293T C821(1.19)  LDD1552  [5]
 LDCM0314  AC51 HEK-293T C821(0.99)  LDD1553  [5]
 LDCM0320  AC57 HEK-293T C821(1.02)  LDD1559  [5]
 LDCM0321  AC58 HEK-293T C821(1.26)  LDD1560  [5]
 LDCM0322  AC59 HEK-293T C821(0.92)  LDD1561  [5]
 LDCM0323  AC6 HCT 116 C816(1.25)  LDD0640  [2]
 LDCM0334  AC7 HCT 116 C816(1.04)  LDD0651  [2]
 LDCM0345  AC8 HCT 116 C816(1.01)  LDD0662  [2]
 LDCM0349  AC83 HCT 116 C816(1.37)  LDD0666  [2]
 LDCM0350  AC84 HCT 116 C816(1.14)  LDD0667  [2]
 LDCM0351  AC85 HCT 116 C816(1.22)  LDD0668  [2]
 LDCM0352  AC86 HCT 116 C816(1.16)  LDD0669  [2]
 LDCM0353  AC87 HCT 116 C816(1.26)  LDD0670  [2]
 LDCM0354  AC88 HCT 116 C816(1.31)  LDD0671  [2]
 LDCM0355  AC89 HCT 116 C816(1.23)  LDD0672  [2]
 LDCM0357  AC90 HCT 116 C816(1.50)  LDD0674  [2]
 LDCM0358  AC91 HCT 116 C816(1.20)  LDD0675  [2]
 LDCM0359  AC92 HCT 116 C816(1.11)  LDD0676  [2]
 LDCM0360  AC93 HCT 116 C816(1.11)  LDD0677  [2]
 LDCM0361  AC94 HCT 116 C816(1.08)  LDD0678  [2]
 LDCM0362  AC95 HCT 116 C816(1.61)  LDD0679  [2]
 LDCM0363  AC96 HCT 116 C816(1.37)  LDD0680  [2]
 LDCM0364  AC97 HCT 116 C816(1.44)  LDD0681  [2]
 LDCM0365  AC98 HCT 116 C816(1.43)  LDD0682  [2]
 LDCM0366  AC99 HCT 116 C816(1.46)  LDD0683  [2]
 LDCM0248  AKOS034007472 HCT 116 C816(1.24)  LDD0565  [2]
 LDCM0356  AKOS034007680 HCT 116 C816(1.18)  LDD0673  [2]
 LDCM0275  AKOS034007705 HCT 116 C816(0.85)  LDD0592  [2]
 LDCM0632  CL-Sc Hep-G2 C821(0.45)  LDD2227  [4]
 LDCM0370  CL101 HCT 116 C816(0.66)  LDD0687  [2]
 LDCM0371  CL102 HCT 116 C816(1.16)  LDD0688  [2]
 LDCM0372  CL103 HCT 116 C816(0.98)  LDD0689  [2]
 LDCM0373  CL104 HCT 116 C816(1.01)  LDD0690  [2]
 LDCM0375  CL106 HEK-293T C310(0.97); C821(1.07)  LDD1579  [5]
 LDCM0380  CL110 HEK-293T C310(1.06); C821(1.14)  LDD1584  [5]
 LDCM0384  CL114 HEK-293T C310(0.92); C821(1.19)  LDD1588  [5]
 LDCM0388  CL118 HEK-293T C310(1.10); C821(1.04)  LDD1592  [5]
 LDCM0393  CL122 HEK-293T C310(0.90); C821(1.00)  LDD1597  [5]
 LDCM0397  CL126 HEK-293T C310(0.85); C821(0.99)  LDD1601  [5]
 LDCM0401  CL14 HEK-293T C310(1.09); C821(1.10)  LDD1605  [5]
 LDCM0403  CL16 HCT 116 C816(0.91)  LDD0720  [2]
 LDCM0404  CL17 HCT 116 C816(0.89)  LDD0721  [2]
 LDCM0405  CL18 HCT 116 C816(0.98)  LDD0722  [2]
 LDCM0406  CL19 HCT 116 C816(0.95)  LDD0723  [2]
 LDCM0407  CL2 HEK-293T C310(1.31); C821(1.12)  LDD1611  [5]
 LDCM0408  CL20 HCT 116 C816(0.78)  LDD0725  [2]
 LDCM0409  CL21 HCT 116 C816(0.66)  LDD0726  [2]
 LDCM0410  CL22 HCT 116 C816(0.54)  LDD0727  [2]
 LDCM0411  CL23 HCT 116 C816(1.03)  LDD0728  [2]
 LDCM0412  CL24 HCT 116 C816(0.93)  LDD0729  [2]
 LDCM0413  CL25 HCT 116 C816(0.96)  LDD0730  [2]
 LDCM0414  CL26 HCT 116 C816(0.98)  LDD0731  [2]
 LDCM0415  CL27 HCT 116 C816(0.96)  LDD0732  [2]
 LDCM0416  CL28 HCT 116 C816(0.92)  LDD0733  [2]
 LDCM0417  CL29 HCT 116 C816(0.76)  LDD0734  [2]
 LDCM0419  CL30 HCT 116 C816(0.75)  LDD0736  [2]
 LDCM0420  CL31 HEK-293T C821(0.98)  LDD1624  [5]
 LDCM0421  CL32 HCT 116 C816(1.09)  LDD0738  [2]
 LDCM0422  CL33 HCT 116 C816(1.08)  LDD0739  [2]
 LDCM0423  CL34 HCT 116 C816(0.89)  LDD0740  [2]
 LDCM0424  CL35 HCT 116 C816(1.09)  LDD0741  [2]
 LDCM0425  CL36 HCT 116 C816(0.99)  LDD0742  [2]
 LDCM0426  CL37 HCT 116 C816(0.95)  LDD0743  [2]
 LDCM0428  CL39 HCT 116 C816(0.99)  LDD0745  [2]
 LDCM0430  CL40 HCT 116 C816(1.04)  LDD0747  [2]
 LDCM0431  CL41 HCT 116 C816(0.97)  LDD0748  [2]
 LDCM0432  CL42 HCT 116 C816(0.77)  LDD0749  [2]
 LDCM0433  CL43 HCT 116 C816(1.14)  LDD0750  [2]
 LDCM0434  CL44 HCT 116 C816(0.81)  LDD0751  [2]
 LDCM0435  CL45 HCT 116 C816(0.91)  LDD0752  [2]
 LDCM0436  CL46 HCT 116 C816(0.86)  LDD0753  [2]
 LDCM0437  CL47 HCT 116 C816(1.10)  LDD0754  [2]
 LDCM0438  CL48 HCT 116 C816(0.88)  LDD0755  [2]
 LDCM0439  CL49 HCT 116 C816(0.91)  LDD0756  [2]
 LDCM0440  CL5 HEK-293T C821(1.09)  LDD1644  [5]
 LDCM0441  CL50 HCT 116 C816(0.82)  LDD0758  [2]
 LDCM0442  CL51 HCT 116 C816(0.81)  LDD0759  [2]
 LDCM0443  CL52 HCT 116 C816(0.90)  LDD0760  [2]
 LDCM0444  CL53 HCT 116 C816(0.85)  LDD0761  [2]
 LDCM0445  CL54 HCT 116 C816(1.12)  LDD0762  [2]
 LDCM0446  CL55 HCT 116 C816(1.02)  LDD0763  [2]
 LDCM0447  CL56 HCT 116 C816(0.84)  LDD0764  [2]
 LDCM0448  CL57 HCT 116 C816(1.00)  LDD0765  [2]
 LDCM0449  CL58 HCT 116 C816(1.26)  LDD0766  [2]
 LDCM0450  CL59 HCT 116 C816(0.96)  LDD0767  [2]
 LDCM0451  CL6 HEK-293T C821(1.28)  LDD1654  [5]
 LDCM0452  CL60 HCT 116 C816(0.89)  LDD0769  [2]
 LDCM0454  CL62 HEK-293T C310(0.99); C821(1.02)  LDD1657  [5]
 LDCM0457  CL65 HEK-293T C821(0.96)  LDD1660  [5]
 LDCM0458  CL66 HEK-293T C821(1.54)  LDD1661  [5]
 LDCM0459  CL67 HEK-293T C821(0.97)  LDD1662  [5]
 LDCM0462  CL7 HEK-293T C821(0.92)  LDD1665  [5]
 LDCM0467  CL74 HEK-293T C310(0.87); C821(0.94)  LDD1670  [5]
 LDCM0470  CL77 HEK-293T C821(1.16)  LDD1673  [5]
 LDCM0471  CL78 HEK-293T C821(1.27)  LDD1674  [5]
 LDCM0472  CL79 HEK-293T C821(1.04)  LDD1675  [5]
 LDCM0480  CL86 HEK-293T C310(0.77); C821(0.97)  LDD1683  [5]
 LDCM0483  CL89 HEK-293T C821(0.93)  LDD1686  [5]
 LDCM0485  CL90 HEK-293T C821(1.15)  LDD1688  [5]
 LDCM0486  CL91 HEK-293T C821(0.98)  LDD1689  [5]
 LDCM0493  CL98 HEK-293T C310(0.95); C821(0.99)  LDD1696  [5]
 LDCM0213  Electrophilic fragment 2 MDA-MB-231 C399(1.73)  LDD1702  [1]
 LDCM0427  Fragment51 HCT 116 C816(0.95)  LDD0744  [2]
 LDCM0022  KB02 786-O C185(3.67)  LDD2247  [6]
 LDCM0023  KB03 MDA-MB-231 C753(14.19); C821(3.75)  LDD1701  [1]
 LDCM0024  KB05 G361 C131(3.00)  LDD3311  [6]
 LDCM0528  N-(4-bromophenyl)-2-cyano-N-phenylacetamide MDA-MB-231 C753(0.59)  LDD2121  [1]
 LDCM0499  Nucleophilic fragment 12b MDA-MB-231 C185(0.86)  LDD2092  [1]
 LDCM0501  Nucleophilic fragment 13b MDA-MB-231 C821(0.87)  LDD2094  [1]
 LDCM0506  Nucleophilic fragment 16a MDA-MB-231 C753(0.88)  LDD2099  [1]
 LDCM0526  Nucleophilic fragment 26a MDA-MB-231 C310(1.59)  LDD2119  [1]
 LDCM0527  Nucleophilic fragment 26b MDA-MB-231 C185(1.08)  LDD2120  [1]
 LDCM0530  Nucleophilic fragment 28a MDA-MB-231 C753(1.02)  LDD2123  [1]
 LDCM0532  Nucleophilic fragment 29a MDA-MB-231 C753(1.13)  LDD2125  [1]
 LDCM0534  Nucleophilic fragment 30a MDA-MB-231 C399(0.82)  LDD2127  [1]
 LDCM0535  Nucleophilic fragment 30b MDA-MB-231 C753(0.35)  LDD2128  [1]
 LDCM0543  Nucleophilic fragment 38 MDA-MB-231 C399(0.94)  LDD2136  [1]
 LDCM0546  Nucleophilic fragment 40 MDA-MB-231 C753(0.90)  LDD2140  [1]
 LDCM0549  Nucleophilic fragment 43 MDA-MB-231 C753(0.65); C185(0.87)  LDD2143  [1]
 LDCM0553  Nucleophilic fragment 6b MDA-MB-231 C753(0.48)  LDD2147  [1]

References

1 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
2 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
3 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
4 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
5 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
6 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840