Details of the Target

General Information of Target

Target ID LDTP06666
Target Name Flap endonuclease GEN homolog 1 (GEN1)
Gene Name GEN1
Gene ID 348654
Synonyms
Flap endonuclease GEN homolog 1; EC 3.1.-.-
3D Structure
Download
2D Sequence (FASTA)
Download
3D Structure (PDB)
Download
Sequence
MGVNDLWQILEPVKQHIPLRNLGGKTIAVDLSLWVCEAQTVKKMMGSVMKPHLRNLFFRI
SYLTQMDVKLVFVMEGEPPKLKADVISKRNQSRYGSSGKSWSQKTGRSHFKSVLRECLHM
LECLGIPWVQAAGEAEAMCAYLNAGGHVDGCLTNDGDTFLYGAQTVYRNFTMNTKDPHVD
CYTMSSIKSKLGLDRDALVGLAILLGCDYLPKGVPGVGKEQALKLIQILKGQSLLQRFNR
WNETSCNSSPQLLVTKKLAHCSVCSHPGSPKDHERNGCRLCKSDKYCEPHDYEYCCPCEW
HRTEHDRQLSEVENNIKKKACCCEGFPFHEVIQEFLLNKDKLVKVIRYQRPDLLLFQRFT
LEKMEWPNHYACEKLLVLLTHYDMIERKLGSRNSNQLQPIRIVKTRIRNGVHCFEIEWEK
PEHYAMEDKQHGEFALLTIEEESLFEAAYPEIVAVYQKQKLEIKGKKQKRIKPKENNLPE
PDEVMSFQSHMTLKPTCEIFHKQNSKLNSGISPDPTLPQESISASLNSLLLPKNTPCLNA
QEQFMSSLRPLAIQQIKAVSKSLISESSQPNTSSHNISVIADLHLSTIDWEGTSFSNSPA
IQRNTFSHDLKSEVESELSAIPDGFENIPEQLSCESERYTANIKKVLDEDSDGISPEEHL
LSGITDLCLQDLPLKERIFTKLSYPQDNLQPDVNLKTLSILSVKESCIANSGSDCTSHLS
KDLPGIPLQNESRDSKILKGDQLLQEDYKVNTSVPYSVSNTVVKTCNVRPPNTALDHSRK
VDMQTTRKILMKKSVCLDRHSSDEQSAPVFGKAKYTTQRMKHSSQKHNSSHFKESGHNKL
SSPKIHIKETEQCVRSYETAENEESCFPDSTKSSLSSLQCHKKENNSGTCLDSPLPLRQR
LKLRFQST
Target Bioclass
Enzyme
Family
XPG/RAD2 endonuclease family, GEN subfamily
Subcellular location
Nucleus
Function
Endonuclease which resolves Holliday junctions (HJs) by the introduction of symmetrically related cuts across the junction point, to produce nicked duplex products in which the nicks can be readily ligated. Four-way DNA intermediates, also known as Holliday junctions, are formed during homologous recombination and DNA repair, and their resolution is necessary for proper chromosome segregation. Cleaves HJs by a nick and counter-nick mechanism involving dual coordinated incisions that lead to the formation of ligatable nicked duplex products. Cleavage of the first strand is rate limiting, while second strand cleavage is rapid. Largely monomeric, dimerizes on the HJ and the first nick occurs upon dimerization at the junction. Efficiently cleaves both single and double HJs contained within large recombination intermediates. Exhibits a weak sequence preference for incision between two G residues that reside in a T-rich region of DNA. Has also endonuclease activity on 5'-flap and replication fork (RF) DNA substrates.
Uniprot ID
Q17RS7
Ensemble ID
ENST00000317402.11
HGNC ID
HGNC:26881

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 8 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
AHL-Pu-1
 Probe Info  		C866(2.34)  LDD0168  [1]
DBIA
 Probe Info  		C707(1.18); C715(1.18)  LDD0078  [2]
IA-alkyne
 Probe Info  		C207(0.00); C853(0.00)  LDD0165  [3]
IPM
 Probe Info  		C246(0.00); C707(0.00); C880(0.00); C537(0.00)  LDD2156  [4]
Phosphinate-6
 Probe Info  		N.A.  LDD0018  [5]
Acrolein
 Probe Info  		N.A.  LDD0217  [6]
AOyne
 Probe Info  		15.00  LDD0443  [7]
NAIA_5
 Probe Info  		N.A.  LDD2223  [8]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0025  4SU-RNA HEK-293T C866(2.34)  LDD0168  [1]
 LDCM0026  4SU-RNA+native RNA HEK-293T C866(2.37)  LDD0169  [1]
 LDCM0214  AC1 PaTu 8988t C853(1.93); C246(1.46)  LDD1093  [2]
 LDCM0215  AC10 HCT 116 C246(0.97)  LDD0532  [2]
 LDCM0216  AC100 HCT 116 C246(0.79)  LDD0533  [2]
 LDCM0217  AC101 HCT 116 C246(0.82)  LDD0534  [2]
 LDCM0218  AC102 HCT 116 C246(0.74)  LDD0535  [2]
 LDCM0219  AC103 HCT 116 C246(0.91)  LDD0536  [2]
 LDCM0220  AC104 HCT 116 C246(0.85)  LDD0537  [2]
 LDCM0221  AC105 HCT 116 C246(0.83)  LDD0538  [2]
 LDCM0222  AC106 HCT 116 C246(0.90)  LDD0539  [2]
 LDCM0223  AC107 HCT 116 C246(1.06)  LDD0540  [2]
 LDCM0224  AC108 HCT 116 C246(0.67)  LDD0541  [2]
 LDCM0225  AC109 HCT 116 C246(0.77)  LDD0542  [2]
 LDCM0226  AC11 HCT 116 C246(1.01)  LDD0543  [2]
 LDCM0227  AC110 HCT 116 C246(0.77)  LDD0544  [2]
 LDCM0228  AC111 HCT 116 C246(0.72)  LDD0545  [2]
 LDCM0229  AC112 HCT 116 C246(0.84)  LDD0546  [2]
 LDCM0230  AC113 HEK-293T C246(0.86)  LDD0828  [2]
 LDCM0231  AC114 HEK-293T C246(0.86)  LDD0829  [2]
 LDCM0232  AC115 HEK-293T C246(0.91)  LDD0830  [2]
 LDCM0233  AC116 HEK-293T C246(1.06)  LDD0831  [2]
 LDCM0234  AC117 HEK-293T C246(0.97)  LDD0832  [2]
 LDCM0235  AC118 HEK-293T C246(0.85)  LDD0833  [2]
 LDCM0236  AC119 HEK-293T C246(0.85)  LDD0834  [2]
 LDCM0237  AC12 HCT 116 C246(0.86)  LDD0554  [2]
 LDCM0238  AC120 HEK-293T C246(1.01)  LDD0836  [2]
 LDCM0239  AC121 HEK-293T C246(0.80)  LDD0837  [2]
 LDCM0240  AC122 HEK-293T C246(0.94)  LDD0838  [2]
 LDCM0241  AC123 HEK-293T C246(0.73)  LDD0839  [2]
 LDCM0242  AC124 HEK-293T C246(0.84)  LDD0840  [2]
 LDCM0243  AC125 HEK-293T C246(1.09)  LDD0841  [2]
 LDCM0244  AC126 HEK-293T C246(1.03)  LDD0842  [2]
 LDCM0245  AC127 HEK-293T C246(1.16)  LDD0843  [2]
 LDCM0246  AC128 HCT 116 C246(1.15)  LDD0563  [2]
 LDCM0247  AC129 HCT 116 C246(1.34)  LDD0564  [2]
 LDCM0249  AC130 HCT 116 C246(1.20)  LDD0566  [2]
 LDCM0250  AC131 HCT 116 C246(0.98)  LDD0567  [2]
 LDCM0251  AC132 HCT 116 C246(1.03)  LDD0568  [2]
 LDCM0252  AC133 HCT 116 C246(1.21)  LDD0569  [2]
 LDCM0253  AC134 HCT 116 C246(1.22)  LDD0570  [2]
 LDCM0254  AC135 HCT 116 C246(1.28)  LDD0571  [2]
 LDCM0255  AC136 HCT 116 C246(1.13)  LDD0572  [2]
 LDCM0256  AC137 HCT 116 C246(1.04)  LDD0573  [2]
 LDCM0257  AC138 HCT 116 C246(1.05)  LDD0574  [2]
 LDCM0258  AC139 HCT 116 C246(1.08)  LDD0575  [2]
 LDCM0259  AC14 HCT 116 C246(1.02)  LDD0576  [2]
 LDCM0260  AC140 HCT 116 C246(1.18)  LDD0577  [2]
 LDCM0261  AC141 HCT 116 C246(1.10)  LDD0578  [2]
 LDCM0262  AC142 HCT 116 C246(1.03)  LDD0579  [2]
 LDCM0263  AC143 PaTu 8988t C323(0.89); C321(0.89)  LDD1142  [2]
 LDCM0264  AC144 PaTu 8988t C323(1.02); C321(1.02)  LDD1143  [2]
 LDCM0265  AC145 PaTu 8988t C323(0.76); C321(0.76)  LDD1144  [2]
 LDCM0266  AC146 PaTu 8988t C323(1.13); C321(1.13)  LDD1145  [2]
 LDCM0267  AC147 PaTu 8988t C323(0.75); C321(0.75)  LDD1146  [2]
 LDCM0268  AC148 PaTu 8988t C323(0.83); C321(0.83)  LDD1147  [2]
 LDCM0269  AC149 PaTu 8988t C323(0.95); C321(0.95)  LDD1148  [2]
 LDCM0270  AC15 HCT 116 C246(1.12)  LDD0587  [2]
 LDCM0271  AC150 PaTu 8988t C323(0.91); C321(0.91)  LDD1150  [2]
 LDCM0272  AC151 PaTu 8988t C323(0.83); C321(0.83)  LDD1151  [2]
 LDCM0273  AC152 PaTu 8988t C323(0.69); C321(0.69)  LDD1152  [2]
 LDCM0274  AC153 PaTu 8988t C323(0.83); C321(0.83)  LDD1153  [2]
 LDCM0621  AC154 PaTu 8988t C323(0.84); C321(0.84)  LDD2166  [2]
 LDCM0622  AC155 PaTu 8988t C323(0.90); C321(0.90)  LDD2167  [2]
 LDCM0623  AC156 PaTu 8988t C323(0.81); C321(0.81)  LDD2168  [2]
 LDCM0624  AC157 PaTu 8988t C323(0.88); C321(0.88)  LDD2169  [2]
 LDCM0276  AC17 PaTu 8988t C246(1.16)  LDD1155  [2]
 LDCM0277  AC18 PaTu 8988t C246(1.07)  LDD1156  [2]
 LDCM0278  AC19 PaTu 8988t C246(1.05)  LDD1157  [2]
 LDCM0279  AC2 PaTu 8988t C853(1.36); C246(1.07)  LDD1158  [2]
 LDCM0280  AC20 PaTu 8988t C246(0.98)  LDD1159  [2]
 LDCM0281  AC21 PaTu 8988t C246(0.94)  LDD1160  [2]
 LDCM0282  AC22 PaTu 8988t C246(0.95)  LDD1161  [2]
 LDCM0283  AC23 PaTu 8988t C246(1.12)  LDD1162  [2]
 LDCM0284  AC24 PaTu 8988t C246(1.08)  LDD1163  [2]
 LDCM0287  AC27 HEK-293T C322(0.95)  LDD1526  [9]
 LDCM0288  AC28 HEK-293T C766(1.19)  LDD1527  [9]
 LDCM0289  AC29 HEK-293T C497(0.94); C766(1.01)  LDD1528  [9]
 LDCM0290  AC3 PaTu 8988t C853(1.97); C246(1.16)  LDD1169  [2]
 LDCM0292  AC31 HEK-293T C766(1.10)  LDD1531  [9]
 LDCM0293  AC32 HEK-293T C766(1.01)  LDD1532  [9]
 LDCM0296  AC35 HCT 116 C246(1.00)  LDD0613  [2]
 LDCM0297  AC36 HCT 116 C246(0.98)  LDD0614  [2]
 LDCM0298  AC37 HCT 116 C246(0.88)  LDD0615  [2]
 LDCM0299  AC38 HCT 116 C246(0.95)  LDD0616  [2]
 LDCM0300  AC39 HCT 116 C246(0.82)  LDD0617  [2]
 LDCM0301  AC4 PaTu 8988t C853(1.62); C246(1.03)  LDD1180  [2]
 LDCM0302  AC40 HCT 116 C246(0.87)  LDD0619  [2]
 LDCM0303  AC41 HCT 116 C246(1.01)  LDD0620  [2]
 LDCM0304  AC42 HCT 116 C246(1.02)  LDD0621  [2]
 LDCM0305  AC43 HCT 116 C246(1.18)  LDD0622  [2]
 LDCM0306  AC44 HCT 116 C246(0.98)  LDD0623  [2]
 LDCM0307  AC45 HCT 116 C246(0.87)  LDD0624  [2]
 LDCM0308  AC46 HCT 116 C246(1.01)  LDD0625  [2]
 LDCM0309  AC47 HCT 116 C246(0.89)  LDD0626  [2]
 LDCM0310  AC48 HCT 116 C246(1.09)  LDD0627  [2]
 LDCM0311  AC49 HCT 116 C246(1.25)  LDD0628  [2]
 LDCM0312  AC5 PaTu 8988t C853(1.64); C246(1.08)  LDD1191  [2]
 LDCM0313  AC50 HCT 116 C246(1.12)  LDD0630  [2]
 LDCM0314  AC51 HCT 116 C246(1.00)  LDD0631  [2]
 LDCM0315  AC52 HCT 116 C246(0.93)  LDD0632  [2]
 LDCM0316  AC53 HCT 116 C246(1.18)  LDD0633  [2]
 LDCM0317  AC54 HCT 116 C246(1.01)  LDD0634  [2]
 LDCM0318  AC55 HCT 116 C246(1.26)  LDD0635  [2]
 LDCM0319  AC56 HCT 116 C246(1.32)  LDD0636  [2]
 LDCM0320  AC57 HCT 116 C246(0.87)  LDD0637  [2]
 LDCM0321  AC58 HCT 116 C246(0.97)  LDD0638  [2]
 LDCM0322  AC59 HCT 116 C246(1.09)  LDD0639  [2]
 LDCM0323  AC6 HCT 116 C246(0.99)  LDD0640  [2]
 LDCM0324  AC60 HCT 116 C246(1.14)  LDD0641  [2]
 LDCM0325  AC61 HCT 116 C246(0.99)  LDD0642  [2]
 LDCM0326  AC62 HCT 116 C246(1.05)  LDD0643  [2]
 LDCM0327  AC63 HCT 116 C246(1.09)  LDD0644  [2]
 LDCM0328  AC64 HCT 116 C246(1.09)  LDD0645  [2]
 LDCM0329  AC65 HCT 116 C246(1.07)  LDD0646  [2]
 LDCM0330  AC66 HCT 116 C246(0.93)  LDD0647  [2]
 LDCM0331  AC67 HCT 116 C246(1.20)  LDD0648  [2]
 LDCM0332  AC68 HEK-293T C246(0.92)  LDD0930  [2]
 LDCM0333  AC69 HEK-293T C246(0.96)  LDD0931  [2]
 LDCM0334  AC7 HCT 116 C246(0.96)  LDD0651  [2]
 LDCM0335  AC70 HEK-293T C246(0.84)  LDD0933  [2]
 LDCM0336  AC71 HEK-293T C246(0.88)  LDD0934  [2]
 LDCM0337  AC72 HEK-293T C246(1.06)  LDD0935  [2]
 LDCM0338  AC73 HEK-293T C246(0.98)  LDD0936  [2]
 LDCM0339  AC74 HEK-293T C246(0.78)  LDD0937  [2]
 LDCM0340  AC75 HEK-293T C246(0.89)  LDD0938  [2]
 LDCM0341  AC76 HEK-293T C246(0.71)  LDD0939  [2]
 LDCM0342  AC77 HEK-293T C246(0.82)  LDD0940  [2]
 LDCM0343  AC78 HEK-293T C246(0.87)  LDD0941  [2]
 LDCM0344  AC79 HEK-293T C246(0.87)  LDD0942  [2]
 LDCM0345  AC8 HCT 116 C246(0.99)  LDD0662  [2]
 LDCM0346  AC80 HEK-293T C246(0.91)  LDD0944  [2]
 LDCM0347  AC81 HEK-293T C246(0.86)  LDD0945  [2]
 LDCM0348  AC82 HEK-293T C246(1.01)  LDD0946  [2]
 LDCM0349  AC83 HCT 116 C246(1.51)  LDD0666  [2]
 LDCM0350  AC84 HCT 116 C246(1.33)  LDD0667  [2]
 LDCM0351  AC85 HCT 116 C246(1.12)  LDD0668  [2]
 LDCM0352  AC86 HCT 116 C246(1.07)  LDD0669  [2]
 LDCM0353  AC87 HCT 116 C246(0.95)  LDD0670  [2]
 LDCM0354  AC88 HCT 116 C246(1.02)  LDD0671  [2]
 LDCM0355  AC89 HCT 116 C246(1.03)  LDD0672  [2]
 LDCM0357  AC90 HCT 116 C246(0.85)  LDD0674  [2]
 LDCM0358  AC91 HCT 116 C246(1.24)  LDD0675  [2]
 LDCM0359  AC92 HCT 116 C246(1.22)  LDD0676  [2]
 LDCM0360  AC93 HCT 116 C246(1.02)  LDD0677  [2]
 LDCM0361  AC94 HCT 116 C246(1.12)  LDD0678  [2]
 LDCM0362  AC95 HCT 116 C246(1.16)  LDD0679  [2]
 LDCM0363  AC96 HCT 116 C246(1.13)  LDD0680  [2]
 LDCM0364  AC97 HCT 116 C246(1.18)  LDD0681  [2]
 LDCM0365  AC98 HCT 116 C246(1.13)  LDD0682  [2]
 LDCM0366  AC99 HCT 116 C246(0.84)  LDD0683  [2]
 LDCM0248  AKOS034007472 HCT 116 C246(1.00)  LDD0565  [2]
 LDCM0356  AKOS034007680 HCT 116 C246(1.04)  LDD0673  [2]
 LDCM0275  AKOS034007705 HCT 116 C246(1.31)  LDD0592  [2]
 LDCM0020  ARS-1620 HCC44 C707(1.18); C715(1.18)  LDD0078  [2]
 LDCM0632  CL-Sc Hep-G2 C853(2.39)  LDD2227  [8]
 LDCM0367  CL1 HEK-293T C246(0.86)  LDD0965  [2]
 LDCM0368  CL10 HEK-293T C246(0.41)  LDD0966  [2]
 LDCM0369  CL100 PaTu 8988t C853(1.75); C246(1.20)  LDD1248  [2]
 LDCM0370  CL101 HCT 116 C246(1.18)  LDD0687  [2]
 LDCM0371  CL102 HCT 116 C246(0.95)  LDD0688  [2]
 LDCM0372  CL103 HCT 116 C246(1.15)  LDD0689  [2]
 LDCM0373  CL104 HCT 116 C246(1.03)  LDD0690  [2]
 LDCM0374  CL105 PaTu 8988t C246(0.99)  LDD1253  [2]
 LDCM0375  CL106 PaTu 8988t C246(1.11)  LDD1254  [2]
 LDCM0376  CL107 PaTu 8988t C246(1.05)  LDD1255  [2]
 LDCM0377  CL108 PaTu 8988t C246(1.10)  LDD1256  [2]
 LDCM0378  CL109 PaTu 8988t C246(1.06)  LDD1257  [2]
 LDCM0379  CL11 HEK-293T C246(0.45)  LDD0977  [2]
 LDCM0380  CL110 PaTu 8988t C246(1.10)  LDD1259  [2]
 LDCM0381  CL111 PaTu 8988t C246(1.13)  LDD1260  [2]
 LDCM0382  CL112 HEK-293T C322(0.92)  LDD1586  [9]
 LDCM0383  CL113 HEK-293T C497(0.95); C880(0.95); C322(1.21)  LDD1587  [9]
 LDCM0385  CL115 HEK-293T C497(1.01)  LDD1589  [9]
 LDCM0386  CL116 HEK-293T C322(0.78)  LDD1590  [9]
 LDCM0387  CL117 HCT 116 C246(1.24)  LDD0704  [2]
 LDCM0388  CL118 HCT 116 C246(1.00)  LDD0705  [2]
 LDCM0389  CL119 HCT 116 C246(1.08)  LDD0706  [2]
 LDCM0390  CL12 HEK-293T C246(0.87)  LDD0988  [2]
 LDCM0391  CL120 HCT 116 C246(1.08)  LDD0708  [2]
 LDCM0392  CL121 HCT 116 C246(0.72)  LDD0709  [2]
 LDCM0393  CL122 HCT 116 C246(1.02)  LDD0710  [2]
 LDCM0394  CL123 HCT 116 C246(1.10)  LDD0711  [2]
 LDCM0395  CL124 HCT 116 C246(1.18)  LDD0712  [2]
 LDCM0396  CL125 HCT 116 C246(1.08)  LDD0713  [2]
 LDCM0397  CL126 HCT 116 C246(1.00)  LDD0714  [2]
 LDCM0398  CL127 HCT 116 C246(1.09)  LDD0715  [2]
 LDCM0399  CL128 HCT 116 C246(1.11)  LDD0716  [2]
 LDCM0400  CL13 HEK-293T C246(0.83)  LDD0998  [2]
 LDCM0401  CL14 HEK-293T C246(0.91)  LDD0999  [2]
 LDCM0402  CL15 HEK-293T C246(0.89)  LDD1000  [2]
 LDCM0403  CL16 HEK-293T C246(1.32)  LDD1001  [2]
 LDCM0404  CL17 HCT 116 C246(1.12)  LDD0721  [2]
 LDCM0405  CL18 HCT 116 C246(0.98)  LDD0722  [2]
 LDCM0406  CL19 HCT 116 C246(1.05)  LDD0723  [2]
 LDCM0407  CL2 HEK-293T C246(0.76)  LDD1005  [2]
 LDCM0408  CL20 HCT 116 C246(1.29)  LDD0725  [2]
 LDCM0409  CL21 HCT 116 C246(0.95)  LDD0726  [2]
 LDCM0410  CL22 HCT 116 C246(1.00)  LDD0727  [2]
 LDCM0411  CL23 HCT 116 C246(1.25)  LDD0728  [2]
 LDCM0412  CL24 HCT 116 C246(0.97)  LDD0729  [2]
 LDCM0413  CL25 HCT 116 C246(0.91)  LDD0730  [2]
 LDCM0414  CL26 HCT 116 C246(0.88)  LDD0731  [2]
 LDCM0415  CL27 HCT 116 C246(1.10)  LDD0732  [2]
 LDCM0416  CL28 HCT 116 C246(0.97)  LDD0733  [2]
 LDCM0417  CL29 HCT 116 C246(1.14)  LDD0734  [2]
 LDCM0418  CL3 HEK-293T C246(0.96)  LDD1016  [2]
 LDCM0419  CL30 HCT 116 C246(1.05)  LDD0736  [2]
 LDCM0420  CL31 HCT 116 C246(0.94)  LDD0737  [2]
 LDCM0421  CL32 HCT 116 C246(1.02)  LDD0738  [2]
 LDCM0422  CL33 HCT 116 C246(1.09)  LDD0739  [2]
 LDCM0423  CL34 HCT 116 C246(1.17)  LDD0740  [2]
 LDCM0424  CL35 HCT 116 C246(1.08)  LDD0741  [2]
 LDCM0425  CL36 HCT 116 C246(1.13)  LDD0742  [2]
 LDCM0426  CL37 HCT 116 C246(1.04)  LDD0743  [2]
 LDCM0428  CL39 HCT 116 C246(0.98)  LDD0745  [2]
 LDCM0429  CL4 HEK-293T C246(0.90)  LDD1027  [2]
 LDCM0430  CL40 HCT 116 C246(1.18)  LDD0747  [2]
 LDCM0431  CL41 HCT 116 C246(1.06)  LDD0748  [2]
 LDCM0432  CL42 HCT 116 C246(0.96)  LDD0749  [2]
 LDCM0433  CL43 HCT 116 C246(1.00)  LDD0750  [2]
 LDCM0434  CL44 HCT 116 C246(0.98)  LDD0751  [2]
 LDCM0435  CL45 HCT 116 C246(1.12)  LDD0752  [2]
 LDCM0436  CL46 HCT 116 C246(0.99)  LDD0753  [2]
 LDCM0437  CL47 HCT 116 C246(1.06)  LDD0754  [2]
 LDCM0438  CL48 HCT 116 C246(0.93)  LDD0755  [2]
 LDCM0439  CL49 HCT 116 C246(1.08)  LDD0756  [2]
 LDCM0440  CL5 HEK-293T C246(0.94)  LDD1038  [2]
 LDCM0441  CL50 HCT 116 C246(1.02)  LDD0758  [2]
 LDCM0442  CL51 HCT 116 C246(1.24)  LDD0759  [2]
 LDCM0443  CL52 HCT 116 C246(1.17)  LDD0760  [2]
 LDCM0444  CL53 HCT 116 C246(0.91)  LDD0761  [2]
 LDCM0445  CL54 HCT 116 C246(1.02)  LDD0762  [2]
 LDCM0446  CL55 HCT 116 C246(0.95)  LDD0763  [2]
 LDCM0447  CL56 HCT 116 C246(0.93)  LDD0764  [2]
 LDCM0448  CL57 HCT 116 C246(1.11)  LDD0765  [2]
 LDCM0449  CL58 HCT 116 C246(1.01)  LDD0766  [2]
 LDCM0450  CL59 HCT 116 C246(1.00)  LDD0767  [2]
 LDCM0451  CL6 HEK-293T C246(0.72)  LDD1049  [2]
 LDCM0452  CL60 HCT 116 C246(1.01)  LDD0769  [2]
 LDCM0453  CL61 HCT 116 C246(0.90)  LDD0770  [2]
 LDCM0454  CL62 HCT 116 C246(0.87)  LDD0771  [2]
 LDCM0455  CL63 HCT 116 C246(0.95)  LDD0772  [2]
 LDCM0456  CL64 HCT 116 C246(0.96)  LDD0773  [2]
 LDCM0457  CL65 HCT 116 C246(0.90)  LDD0774  [2]
 LDCM0458  CL66 HCT 116 C246(0.89)  LDD0775  [2]
 LDCM0459  CL67 HCT 116 C246(0.85)  LDD0776  [2]
 LDCM0460  CL68 HCT 116 C246(0.90)  LDD0777  [2]
 LDCM0461  CL69 HCT 116 C246(1.13)  LDD0778  [2]
 LDCM0462  CL7 HEK-293T C246(0.71)  LDD1060  [2]
 LDCM0463  CL70 HCT 116 C246(1.02)  LDD0780  [2]
 LDCM0464  CL71 HCT 116 C246(0.93)  LDD0781  [2]
 LDCM0465  CL72 HCT 116 C246(1.05)  LDD0782  [2]
 LDCM0466  CL73 HCT 116 C246(1.02)  LDD0783  [2]
 LDCM0467  CL74 HCT 116 C246(1.01)  LDD0784  [2]
 LDCM0469  CL76 PaTu 8988t C323(0.74); C321(0.74); C246(1.11)  LDD1348  [2]
 LDCM0470  CL77 PaTu 8988t C323(1.09); C321(1.09); C246(1.28)  LDD1349  [2]
 LDCM0471  CL78 PaTu 8988t C323(0.77); C321(0.77); C246(1.19)  LDD1350  [2]
 LDCM0472  CL79 PaTu 8988t C323(0.75); C321(0.75); C246(1.24)  LDD1351  [2]
 LDCM0473  CL8 HEK-293T C246(1.13)  LDD1071  [2]
 LDCM0474  CL80 PaTu 8988t C323(0.95); C321(0.95); C246(1.16)  LDD1353  [2]
 LDCM0475  CL81 PaTu 8988t C323(0.60); C321(0.60); C246(1.21)  LDD1354  [2]
 LDCM0476  CL82 PaTu 8988t C323(0.87); C321(0.87); C246(1.23)  LDD1355  [2]
 LDCM0477  CL83 PaTu 8988t C323(0.86); C321(0.86); C246(1.48)  LDD1356  [2]
 LDCM0478  CL84 PaTu 8988t C323(0.82); C321(0.82); C246(1.21)  LDD1357  [2]
 LDCM0479  CL85 PaTu 8988t C323(0.72); C321(0.72); C246(1.13)  LDD1358  [2]
 LDCM0480  CL86 PaTu 8988t C323(0.83); C321(0.83); C246(1.09)  LDD1359  [2]
 LDCM0481  CL87 PaTu 8988t C323(0.88); C321(0.88); C246(1.22)  LDD1360  [2]
 LDCM0482  CL88 PaTu 8988t C323(0.82); C321(0.82); C246(1.32)  LDD1361  [2]
 LDCM0483  CL89 PaTu 8988t C323(0.69); C321(0.69); C246(1.15)  LDD1362  [2]
 LDCM0484  CL9 HEK-293T C246(1.31)  LDD1082  [2]
 LDCM0485  CL90 PaTu 8988t C323(0.62); C321(0.62); C246(1.41)  LDD1364  [2]
 LDCM0486  CL91 PaTu 8988t C853(1.73); C246(1.28)  LDD1365  [2]
 LDCM0487  CL92 PaTu 8988t C853(1.76); C246(1.01)  LDD1366  [2]
 LDCM0488  CL93 PaTu 8988t C853(1.30); C246(0.90)  LDD1367  [2]
 LDCM0489  CL94 PaTu 8988t C853(1.15); C246(1.13)  LDD1368  [2]
 LDCM0490  CL95 PaTu 8988t C853(1.92); C246(1.41)  LDD1369  [2]
 LDCM0491  CL96 PaTu 8988t C853(1.81); C246(1.16)  LDD1370  [2]
 LDCM0492  CL97 PaTu 8988t C853(1.38); C246(1.30)  LDD1371  [2]
 LDCM0493  CL98 PaTu 8988t C853(2.24); C246(1.16)  LDD1372  [2]
 LDCM0494  CL99 PaTu 8988t C853(1.66); C246(1.04)  LDD1373  [2]
 LDCM0495  E2913 HEK-293T C497(1.18)  LDD1698  [9]
 LDCM0575  Fragment13 Ramos C766(1.62)  LDD2192  [10]
 LDCM0576  Fragment14 Ramos C766(0.56)  LDD2193  [10]
 LDCM0580  Fragment21 Ramos C766(0.87)  LDD2195  [10]
 LDCM0582  Fragment23 Ramos C766(3.37)  LDD2196  [10]
 LDCM0588  Fragment30 Ramos C766(2.01)  LDD2199  [10]
 LDCM0468  Fragment33 HCT 116 C246(0.99)  LDD0785  [2]
 LDCM0427  Fragment51 HCT 116 C246(1.51)  LDD0744  [2]
 LDCM0022  KB02 786-O C853(2.16)  LDD2247  [11]
 LDCM0023  KB03 Jurkat C853(4.91)  LDD0209  [12]
 LDCM0024  KB05 UACC257 C853(1.19)  LDD3325  [11]

References

1 Chemoproteomic capture of RNA binding activity in living cells. Nat Commun. 2023 Oct 7;14(1):6282. doi: 10.1038/s41467-023-41844-z.
Mass spectrometry data entry: PXD044625
2 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
3 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
4 Benchmarking Cleavable Biotin Tags for Peptide-Centric Chemoproteomics. J Proteome Res. 2022 May 6;21(5):1349-1358. doi: 10.1021/acs.jproteome.2c00174. Epub 2022 Apr 25.
Mass spectrometry data entry: PXD031019
5 DFT-Guided Discovery of Ethynyl-Triazolyl-Phosphinates as Modular Electrophiles for Chemoselective Cysteine Bioconjugation and Profiling. Angew Chem Int Ed Engl. 2022 Oct 10;61(41):e202205348. doi: 10.1002/anie.202205348. Epub 2022 Aug 22.
Mass spectrometry data entry: PXD033004
6 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
7 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
8 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
9 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
10 Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry. Methods Enzymol. 2023;679:295-322. doi: 10.1016/bs.mie.2022.07.037. Epub 2022 Sep 7.
Mass spectrometry data entry: PXD027578
11 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
12 Covalent Inhibition by a Natural Product-Inspired Latent Electrophile. J Am Chem Soc. 2023 May 24;145(20):11097-11109. doi: 10.1021/jacs.3c00598. Epub 2023 May 15.