Details of the Target

General Information of Target

Target ID LDTP05561
Target Name Lactadherin (MFGE8)
Gene Name MFGE8
Gene ID 4240
Synonyms
Lactadherin; Breast epithelial antigen BA46; HMFG; MFGM; Milk fat globule-EGF factor 8; MFG-E8; SED1) [Cleaved into: Lactadherin short form; Medin]
3D Structure
Download
2D Sequence (FASTA)
Download
3D Structure (PDB)
Download
Sequence
MPRPRLLAALCGALLCAPSLLVALDICSKNPCHNGGLCEEISQEVRGDVFPSYTCTCLKG
YAGNHCETKCVEPLGLENGNIANSQIAASSVRVTFLGLQHWVPELARLNRAGMVNAWTPS
SNDDNPWIQVNLLRRMWVTGVVTQGASRLASHEYLKAFKVAYSLNGHEFDFIHDVNKKHK
EFVGNWNKNAVHVNLFETPVEAQYVRLYPTSCHTACTLRFELLGCELNGCANPLGLKNNS
IPDKQITASSSYKTWGLHLFSWNPSYARLDKQGNFNAWVAGSYGNDQWLQVDLGSSKEVT
GIITQGARNFGSVQFVASYKVAYSNDSANWTEYQDPRTGSSKIFPGNWDNHSHKKNLFET
PILARYVRILPVAWHNRIALRLELLGC
Target Type
Literature-reported
Target Bioclass
Enzyme
Subcellular location
Membrane
Function
Plays an important role in the maintenance of intestinal epithelial homeostasis and the promotion of mucosal healing. Promotes VEGF-dependent neovascularization. Contributes to phagocytic removal of apoptotic cells in many tissues. Specific ligand for the alpha-v/beta-3 and alpha-v/beta-5 receptors. Also binds to phosphatidylserine-enriched cell surfaces in a receptor-independent manner. Zona pellucida-binding protein which may play a role in gamete interaction.; [Medin]: Main constituent of aortic medial amyloid.
TTD ID
T22538
Uniprot ID
Q08431
DrugMap ID
TT1GLAJ
Ensemble ID
ENST00000268150.13
HGNC ID
HGNC:7036
ChEMBL ID
CHEMBL3713343

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
AGS SNV: p.V279A .
COLO792 SNV: p.P4T DBIA    Probe Info 
HSC4 SNV: p.P199R .
Ishikawa (Heraklio) 02 ER SNV: p.W117Ter .
MCC13 SNV: p.P242S .
MOLT4 SNV: p.W255R IA-alkyne    Probe Info 
NALM6 SNV: p.G386D .
NCIH2286 SNV: p.A111S .
SUPT1 SNV: p.L380M .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 7 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
TH211
 Probe Info  		Y252(20.00)  LDD0260  [1]
STPyne
 Probe Info  		K355(9.89)  LDD0277  [2]
DBIA
 Probe Info  		C66(2.53)  LDD3310  [3]
BTD
 Probe Info  		C216(1.23)  LDD1700  [4]
IA-alkyne
 Probe Info  		C32(0.00); C38(0.00)  LDD0036  [5]
Lodoacetamide azide
 Probe Info  		C216(0.00); C212(0.00)  LDD0037  [5]
AOyne
 Probe Info  		15.00  LDD0443  [6]
PAL-AfBPP Probe
Click To Hide/Show 6 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C108
 Probe Info  		8.28  LDD1795  [7]
C149
 Probe Info  		5.50  LDD1830  [7]
C170
 Probe Info  		17.27  LDD1850  [7]
C281
 Probe Info  		10.56  LDD1951  [7]
C302
 Probe Info  		11.88  LDD1971  [7]
C310
 Probe Info  		10.13  LDD1977  [7]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0502  1-(Cyanoacetyl)piperidine MDA-MB-231 C216(0.86)  LDD2095  [4]
 LDCM0215  AC10 HEK-293T C230(1.01)  LDD1508  [8]
 LDCM0226  AC11 HEK-293T C66(0.96); C230(0.93)  LDD1509  [8]
 LDCM0237  AC12 HEK-293T C66(1.12)  LDD1510  [8]
 LDCM0259  AC14 HEK-293T C230(1.03)  LDD1512  [8]
 LDCM0270  AC15 HEK-293T C230(1.02)  LDD1513  [8]
 LDCM0277  AC18 HEK-293T C230(1.06)  LDD1516  [8]
 LDCM0278  AC19 HEK-293T C66(1.37); C230(1.03)  LDD1517  [8]
 LDCM0279  AC2 HEK-293T C230(1.05)  LDD1518  [8]
 LDCM0280  AC20 HEK-293T C66(1.36)  LDD1519  [8]
 LDCM0281  AC21 HEK-293T C230(0.99)  LDD1520  [8]
 LDCM0282  AC22 HEK-293T C230(1.00)  LDD1521  [8]
 LDCM0283  AC23 HEK-293T C230(0.98)  LDD1522  [8]
 LDCM0286  AC26 HEK-293T C230(0.96)  LDD1525  [8]
 LDCM0287  AC27 HEK-293T C66(0.87); C230(0.99)  LDD1526  [8]
 LDCM0288  AC28 HEK-293T C66(1.15)  LDD1527  [8]
 LDCM0289  AC29 HEK-293T C230(0.87)  LDD1528  [8]
 LDCM0290  AC3 HEK-293T C66(0.83); C230(0.96)  LDD1529  [8]
 LDCM0291  AC30 HEK-293T C230(1.03)  LDD1530  [8]
 LDCM0292  AC31 HEK-293T C230(1.04)  LDD1531  [8]
 LDCM0295  AC34 HEK-293T C230(0.93)  LDD1534  [8]
 LDCM0296  AC35 HEK-293T C66(0.93); C230(0.97)  LDD1535  [8]
 LDCM0297  AC36 HEK-293T C66(1.04)  LDD1536  [8]
 LDCM0298  AC37 HEK-293T C230(0.99)  LDD1537  [8]
 LDCM0299  AC38 HEK-293T C230(1.19)  LDD1538  [8]
 LDCM0300  AC39 HEK-293T C230(1.08)  LDD1539  [8]
 LDCM0301  AC4 HEK-293T C66(1.08)  LDD1540  [8]
 LDCM0304  AC42 HEK-293T C230(0.90)  LDD1543  [8]
 LDCM0305  AC43 HEK-293T C66(1.00); C230(1.01)  LDD1544  [8]
 LDCM0306  AC44 HEK-293T C66(0.90)  LDD1545  [8]
 LDCM0307  AC45 HEK-293T C230(1.16)  LDD1546  [8]
 LDCM0308  AC46 HEK-293T C230(0.98)  LDD1547  [8]
 LDCM0309  AC47 HEK-293T C230(0.82)  LDD1548  [8]
 LDCM0312  AC5 HEK-293T C230(0.83)  LDD1551  [8]
 LDCM0313  AC50 HEK-293T C230(0.97)  LDD1552  [8]
 LDCM0314  AC51 HEK-293T C66(1.06); C230(0.91)  LDD1553  [8]
 LDCM0315  AC52 HEK-293T C66(1.02)  LDD1554  [8]
 LDCM0316  AC53 HEK-293T C230(1.20)  LDD1555  [8]
 LDCM0317  AC54 HEK-293T C230(0.84)  LDD1556  [8]
 LDCM0318  AC55 HEK-293T C230(1.16)  LDD1557  [8]
 LDCM0321  AC58 HEK-293T C230(1.02)  LDD1560  [8]
 LDCM0322  AC59 HEK-293T C66(0.96); C230(0.97)  LDD1561  [8]
 LDCM0323  AC6 HEK-293T C230(1.19)  LDD1562  [8]
 LDCM0324  AC60 HEK-293T C66(1.10)  LDD1563  [8]
 LDCM0325  AC61 HEK-293T C230(1.01)  LDD1564  [8]
 LDCM0326  AC62 HEK-293T C230(1.17)  LDD1565  [8]
 LDCM0327  AC63 HEK-293T C230(1.14)  LDD1566  [8]
 LDCM0334  AC7 HEK-293T C230(1.02)  LDD1568  [8]
 LDCM0248  AKOS034007472 HEK-293T C230(1.06)  LDD1511  [8]
 LDCM0368  CL10 HEK-293T C230(0.98)  LDD1572  [8]
 LDCM0369  CL100 HEK-293T C230(0.99)  LDD1573  [8]
 LDCM0371  CL102 HEK-293T C225(0.61)  LDD1575  [8]
 LDCM0372  CL103 HEK-293T C230(1.15)  LDD1576  [8]
 LDCM0373  CL104 HEK-293T C230(0.91)  LDD1577  [8]
 LDCM0375  CL106 HEK-293T C225(1.83)  LDD1579  [8]
 LDCM0376  CL107 HEK-293T C230(1.21)  LDD1580  [8]
 LDCM0377  CL108 HEK-293T C230(0.98)  LDD1581  [8]
 LDCM0379  CL11 HEK-293T C230(1.01)  LDD1583  [8]
 LDCM0380  CL110 HEK-293T C225(1.16)  LDD1584  [8]
 LDCM0381  CL111 HEK-293T C230(0.98)  LDD1585  [8]
 LDCM0382  CL112 HEK-293T C230(0.96)  LDD1586  [8]
 LDCM0384  CL114 HEK-293T C225(0.06)  LDD1588  [8]
 LDCM0385  CL115 HEK-293T C230(1.16)  LDD1589  [8]
 LDCM0386  CL116 HEK-293T C230(0.88)  LDD1590  [8]
 LDCM0388  CL118 HEK-293T C225(1.11)  LDD1592  [8]
 LDCM0389  CL119 HEK-293T C230(1.22)  LDD1593  [8]
 LDCM0391  CL120 HEK-293T C230(0.86)  LDD1595  [8]
 LDCM0393  CL122 HEK-293T C225(0.92)  LDD1597  [8]
 LDCM0394  CL123 HEK-293T C230(1.03)  LDD1598  [8]
 LDCM0395  CL124 HEK-293T C230(0.82)  LDD1599  [8]
 LDCM0397  CL126 HEK-293T C225(0.74)  LDD1601  [8]
 LDCM0398  CL127 HEK-293T C230(1.09)  LDD1602  [8]
 LDCM0399  CL128 HEK-293T C230(1.00)  LDD1603  [8]
 LDCM0401  CL14 HEK-293T C225(0.76)  LDD1605  [8]
 LDCM0402  CL15 HEK-293T C230(1.01)  LDD1606  [8]
 LDCM0403  CL16 HEK-293T C230(0.99)  LDD1607  [8]
 LDCM0405  CL18 HEK-293T C230(1.07)  LDD1609  [8]
 LDCM0406  CL19 HEK-293T C66(0.94); C230(0.98)  LDD1610  [8]
 LDCM0407  CL2 HEK-293T C225(0.66)  LDD1611  [8]
 LDCM0408  CL20 HEK-293T C66(0.94)  LDD1612  [8]
 LDCM0409  CL21 HEK-293T C230(1.02)  LDD1613  [8]
 LDCM0410  CL22 HEK-293T C230(0.97)  LDD1614  [8]
 LDCM0411  CL23 HEK-293T C230(1.03)  LDD1615  [8]
 LDCM0414  CL26 HEK-293T C225(0.79)  LDD1618  [8]
 LDCM0415  CL27 HEK-293T C230(1.07)  LDD1619  [8]
 LDCM0416  CL28 HEK-293T C230(1.00)  LDD1620  [8]
 LDCM0418  CL3 HEK-293T C230(0.98)  LDD1622  [8]
 LDCM0419  CL30 HEK-293T C230(1.04)  LDD1623  [8]
 LDCM0420  CL31 HEK-293T C66(0.88); C230(0.95)  LDD1624  [8]
 LDCM0421  CL32 HEK-293T C66(1.11)  LDD1625  [8]
 LDCM0422  CL33 HEK-293T C230(1.03)  LDD1626  [8]
 LDCM0423  CL34 HEK-293T C230(1.19)  LDD1627  [8]
 LDCM0424  CL35 HEK-293T C230(0.86)  LDD1628  [8]
 LDCM0428  CL39 HEK-293T C230(1.11)  LDD1632  [8]
 LDCM0429  CL4 HEK-293T C230(0.95)  LDD1633  [8]
 LDCM0430  CL40 HEK-293T C230(1.01)  LDD1634  [8]
 LDCM0432  CL42 HEK-293T C230(1.01)  LDD1636  [8]
 LDCM0433  CL43 HEK-293T C66(1.13); C230(0.87)  LDD1637  [8]
 LDCM0434  CL44 HEK-293T C66(1.10)  LDD1638  [8]
 LDCM0435  CL45 HEK-293T C230(0.97)  LDD1639  [8]
 LDCM0436  CL46 HEK-293T C230(0.99)  LDD1640  [8]
 LDCM0437  CL47 HEK-293T C230(0.88)  LDD1641  [8]
 LDCM0441  CL50 HEK-293T C225(1.07)  LDD1645  [8]
 LDCM0443  CL52 HEK-293T C230(0.92)  LDD1646  [8]
 LDCM0445  CL54 HEK-293T C230(0.99)  LDD1648  [8]
 LDCM0446  CL55 HEK-293T C66(1.15); C230(0.97)  LDD1649  [8]
 LDCM0447  CL56 HEK-293T C66(0.82)  LDD1650  [8]
 LDCM0448  CL57 HEK-293T C230(1.02)  LDD1651  [8]
 LDCM0449  CL58 HEK-293T C230(1.09)  LDD1652  [8]
 LDCM0450  CL59 HEK-293T C230(0.99)  LDD1653  [8]
 LDCM0451  CL6 HEK-293T C230(0.96)  LDD1654  [8]
 LDCM0454  CL62 HEK-293T C225(0.93)  LDD1657  [8]
 LDCM0455  CL63 HEK-293T C230(1.14)  LDD1658  [8]
 LDCM0456  CL64 HEK-293T C230(0.94)  LDD1659  [8]
 LDCM0458  CL66 HEK-293T C230(1.08)  LDD1661  [8]
 LDCM0459  CL67 HEK-293T C66(1.04); C230(1.05)  LDD1662  [8]
 LDCM0460  CL68 HEK-293T C66(1.24)  LDD1663  [8]
 LDCM0461  CL69 HEK-293T C230(1.15)  LDD1664  [8]
 LDCM0462  CL7 HEK-293T C66(1.06); C230(0.92)  LDD1665  [8]
 LDCM0463  CL70 HEK-293T C230(1.12)  LDD1666  [8]
 LDCM0464  CL71 HEK-293T C230(1.08)  LDD1667  [8]
 LDCM0467  CL74 HEK-293T C225(0.86)  LDD1670  [8]
 LDCM0469  CL76 HEK-293T C230(0.91)  LDD1672  [8]
 LDCM0471  CL78 HEK-293T C230(0.96)  LDD1674  [8]
 LDCM0472  CL79 HEK-293T C66(1.00); C230(0.97)  LDD1675  [8]
 LDCM0473  CL8 HEK-293T C66(1.09)  LDD1676  [8]
 LDCM0474  CL80 HEK-293T C66(1.14)  LDD1677  [8]
 LDCM0475  CL81 HEK-293T C230(1.11)  LDD1678  [8]
 LDCM0476  CL82 HEK-293T C230(0.86)  LDD1679  [8]
 LDCM0477  CL83 HEK-293T C230(0.94)  LDD1680  [8]
 LDCM0480  CL86 HEK-293T C225(0.92)  LDD1683  [8]
 LDCM0481  CL87 HEK-293T C230(1.22)  LDD1684  [8]
 LDCM0482  CL88 HEK-293T C230(1.01)  LDD1685  [8]
 LDCM0484  CL9 HEK-293T C230(1.01)  LDD1687  [8]
 LDCM0485  CL90 HEK-293T C230(0.91)  LDD1688  [8]
 LDCM0486  CL91 HEK-293T C66(0.93); C230(1.00)  LDD1689  [8]
 LDCM0487  CL92 HEK-293T C66(1.09)  LDD1690  [8]
 LDCM0488  CL93 HEK-293T C230(1.02)  LDD1691  [8]
 LDCM0489  CL94 HEK-293T C230(1.15)  LDD1692  [8]
 LDCM0490  CL95 HEK-293T C230(1.30)  LDD1693  [8]
 LDCM0493  CL98 HEK-293T C225(0.74)  LDD1696  [8]
 LDCM0494  CL99 HEK-293T C230(1.10)  LDD1697  [8]
 LDCM0495  E2913 HEK-293T C230(1.28)  LDD1698  [8]
 LDCM0468  Fragment33 HEK-293T C230(1.09)  LDD1671  [8]
 LDCM0427  Fragment51 HEK-293T C225(1.10)  LDD1631  [8]
 LDCM0022  KB02 HEK-293T C212(0.99)  LDD1492  [8]
 LDCM0023  KB03 HEK-293T C212(0.96)  LDD1497  [8]
 LDCM0024  KB05 COLO792 C66(2.53)  LDD3310  [3]
 LDCM0514  Nucleophilic fragment 20a MDA-MB-231 C216(0.66)  LDD2107  [4]
 LDCM0534  Nucleophilic fragment 30a MDA-MB-231 C216(1.26)  LDD2127  [4]
 LDCM0543  Nucleophilic fragment 38 MDA-MB-231 C216(0.59)  LDD2136  [4]
 LDCM0211  Nucleophilic fragment 3b MDA-MB-231 C216(1.23)  LDD1700  [4]

References

1 Chemoproteomic profiling of kinases in live cells using electrophilic sulfonyl triazole probes. Chem Sci. 2021 Jan 21;12(9):3295-3307. doi: 10.1039/d0sc06623k.
2 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
3 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
4 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
5 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
6 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
7 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587
8 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402