Details of the Target

General Information of Target

Target ID LDTP05045
Target Name Ubiquitin-conjugating enzyme E2 B (UBE2B)
Gene Name UBE2B
Gene ID 7320
Synonyms
RAD6B; UBCH1; Ubiquitin-conjugating enzyme E2 B; EC 2.3.2.23; E2 ubiquitin-conjugating enzyme B; RAD6 homolog B; HR6B; hHR6B; Ubiquitin carrier protein B; Ubiquitin-conjugating enzyme E2-17 kDa; Ubiquitin-protein ligase B
3D Structure
Download
2D Sequence (FASTA)
Download
3D Structure (PDB)
Download
Sequence
MSTPARRRLMRDFKRLQEDPPVGVSGAPSENNIMQWNAVIFGPEGTPFEDGTFKLVIEFS
EEYPNKPPTVRFLSKMFHPNVYADGSICLDILQNRWSPTYDVSSILTSIQSLLDEPNPNS
PANSQAAQLYQENKREYEKRVSAIVEQSWNDS
Target Bioclass
Enzyme
Family
Ubiquitin-conjugating enzyme family
Subcellular location
Cell membrane
Function
Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In association with the E3 enzyme BRE1 (RNF20 and/or RNF40), it plays a role in transcription regulation by catalyzing the monoubiquitination of histone H2B at 'Lys-120' to form H2BK120ub1. H2BK120ub1 gives a specific tag for epigenetic transcriptional activation, elongation by RNA polymerase II, telomeric silencing, and is also a prerequisite for H3K4me and H3K79me formation. In vitro catalyzes 'Lys-11'-, as well as 'Lys-48'- and 'Lys-63'-linked polyubiquitination. Required for postreplication repair of UV-damaged DNA. Associates to the E3 ligase RAD18 to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys-164'. May be involved in neurite outgrowth. May play a role in DNA repair.
Uniprot ID
P63146
Ensemble ID
ENST00000265339.7
HGNC ID
HGNC:12473
ChEMBL ID
CHEMBL3784907

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 6 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
HHS-475
 Probe Info  		Y82(2.45)  LDD0264  [1]
DBIA
 Probe Info  		C88(1.12)  LDD0080  [2]
NAIA_4
 Probe Info  		N.A.  LDD2226  [3]
IA-alkyne
 Probe Info  		N.A.  LDD0150  [4]
IPM
 Probe Info  		N.A.  LDD0005  [5]
AOyne
 Probe Info  		12.80  LDD0443  [6]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0214  AC1 HCT 116 C88(1.23)  LDD0531  [2]
 LDCM0215  AC10 HCT 116 C88(1.42)  LDD0532  [2]
 LDCM0216  AC100 PaTu 8988t C88(1.04)  LDD1095  [2]
 LDCM0217  AC101 PaTu 8988t C88(0.97)  LDD1096  [2]
 LDCM0218  AC102 PaTu 8988t C88(1.16)  LDD1097  [2]
 LDCM0219  AC103 PaTu 8988t C88(1.24)  LDD1098  [2]
 LDCM0220  AC104 PaTu 8988t C88(0.87)  LDD1099  [2]
 LDCM0221  AC105 PaTu 8988t C88(1.35)  LDD1100  [2]
 LDCM0222  AC106 PaTu 8988t C88(0.98)  LDD1101  [2]
 LDCM0223  AC107 PaTu 8988t C88(0.97)  LDD1102  [2]
 LDCM0224  AC108 PaTu 8988t C88(0.92)  LDD1103  [2]
 LDCM0225  AC109 PaTu 8988t C88(1.06)  LDD1104  [2]
 LDCM0226  AC11 HCT 116 C88(1.05)  LDD0543  [2]
 LDCM0227  AC110 PaTu 8988t C88(1.54)  LDD1106  [2]
 LDCM0228  AC111 PaTu 8988t C88(1.22)  LDD1107  [2]
 LDCM0229  AC112 PaTu 8988t C88(1.05)  LDD1108  [2]
 LDCM0230  AC113 HCT 116 C88(0.90)  LDD0547  [2]
 LDCM0231  AC114 HCT 116 C88(0.93)  LDD0548  [2]
 LDCM0232  AC115 HCT 116 C88(1.46)  LDD0549  [2]
 LDCM0233  AC116 HCT 116 C88(1.32)  LDD0550  [2]
 LDCM0234  AC117 HCT 116 C88(1.17)  LDD0551  [2]
 LDCM0235  AC118 HCT 116 C88(1.02)  LDD0552  [2]
 LDCM0236  AC119 HCT 116 C88(1.06)  LDD0553  [2]
 LDCM0237  AC12 HCT 116 C88(1.03)  LDD0554  [2]
 LDCM0238  AC120 HCT 116 C88(1.43)  LDD0555  [2]
 LDCM0239  AC121 HCT 116 C88(0.99)  LDD0556  [2]
 LDCM0240  AC122 HCT 116 C88(0.83)  LDD0557  [2]
 LDCM0241  AC123 HCT 116 C88(1.00)  LDD0558  [2]
 LDCM0242  AC124 HCT 116 C88(1.01)  LDD0559  [2]
 LDCM0243  AC125 HCT 116 C88(1.21)  LDD0560  [2]
 LDCM0244  AC126 HCT 116 C88(0.99)  LDD0561  [2]
 LDCM0245  AC127 HCT 116 C88(1.18)  LDD0562  [2]
 LDCM0246  AC128 PaTu 8988t C88(0.64)  LDD1125  [2]
 LDCM0247  AC129 PaTu 8988t C88(1.06)  LDD1126  [2]
 LDCM0249  AC130 PaTu 8988t C88(0.97)  LDD1128  [2]
 LDCM0250  AC131 PaTu 8988t C88(0.76)  LDD1129  [2]
 LDCM0251  AC132 PaTu 8988t C88(1.00)  LDD1130  [2]
 LDCM0252  AC133 PaTu 8988t C88(0.85)  LDD1131  [2]
 LDCM0253  AC134 PaTu 8988t C88(0.95)  LDD1132  [2]
 LDCM0254  AC135 PaTu 8988t C88(0.75)  LDD1133  [2]
 LDCM0255  AC136 PaTu 8988t C88(0.57)  LDD1134  [2]
 LDCM0256  AC137 PaTu 8988t C88(0.77)  LDD1135  [2]
 LDCM0257  AC138 PaTu 8988t C88(0.89)  LDD1136  [2]
 LDCM0258  AC139 PaTu 8988t C88(0.55)  LDD1137  [2]
 LDCM0259  AC14 HCT 116 C88(1.10)  LDD0576  [2]
 LDCM0260  AC140 PaTu 8988t C88(0.92)  LDD1139  [2]
 LDCM0261  AC141 PaTu 8988t C88(1.09)  LDD1140  [2]
 LDCM0262  AC142 PaTu 8988t C88(0.95)  LDD1141  [2]
 LDCM0270  AC15 HCT 116 C88(1.24)  LDD0587  [2]
 LDCM0276  AC17 HCT 116 C88(0.89)  LDD0593  [2]
 LDCM0277  AC18 HCT 116 C88(0.71)  LDD0594  [2]
 LDCM0278  AC19 HCT 116 C88(0.74)  LDD0595  [2]
 LDCM0279  AC2 HCT 116 C88(1.14)  LDD0596  [2]
 LDCM0280  AC20 HCT 116 C88(0.94)  LDD0597  [2]
 LDCM0281  AC21 HCT 116 C88(0.86)  LDD0598  [2]
 LDCM0282  AC22 HCT 116 C88(0.74)  LDD0599  [2]
 LDCM0283  AC23 HCT 116 C88(0.76)  LDD0600  [2]
 LDCM0284  AC24 HCT 116 C88(0.89)  LDD0601  [2]
 LDCM0285  AC25 HCT 116 C88(0.77)  LDD0602  [2]
 LDCM0286  AC26 HCT 116 C88(0.70)  LDD0603  [2]
 LDCM0287  AC27 HCT 116 C88(0.80)  LDD0604  [2]
 LDCM0288  AC28 HCT 116 C88(0.68)  LDD0605  [2]
 LDCM0289  AC29 HCT 116 C88(1.11)  LDD0606  [2]
 LDCM0290  AC3 HCT 116 C88(1.05)  LDD0607  [2]
 LDCM0291  AC30 HCT 116 C88(1.31)  LDD0608  [2]
 LDCM0292  AC31 HCT 116 C88(0.97)  LDD0609  [2]
 LDCM0293  AC32 HCT 116 C88(1.10)  LDD0610  [2]
 LDCM0294  AC33 HCT 116 C88(0.88)  LDD0611  [2]
 LDCM0295  AC34 HCT 116 C88(1.24)  LDD0612  [2]
 LDCM0301  AC4 HCT 116 C88(1.28)  LDD0618  [2]
 LDCM0308  AC46 HCT 116 C88(0.95)  LDD0625  [2]
 LDCM0309  AC47 HCT 116 C88(0.90)  LDD0626  [2]
 LDCM0310  AC48 HCT 116 C88(0.90)  LDD0627  [2]
 LDCM0311  AC49 HCT 116 C88(0.82)  LDD0628  [2]
 LDCM0312  AC5 HCT 116 C88(1.26)  LDD0629  [2]
 LDCM0313  AC50 HCT 116 C88(1.06)  LDD0630  [2]
 LDCM0314  AC51 HCT 116 C88(0.87)  LDD0631  [2]
 LDCM0315  AC52 HCT 116 C88(1.00)  LDD0632  [2]
 LDCM0316  AC53 HCT 116 C88(0.71)  LDD0633  [2]
 LDCM0317  AC54 HCT 116 C88(0.81)  LDD0634  [2]
 LDCM0318  AC55 HCT 116 C88(0.74)  LDD0635  [2]
 LDCM0319  AC56 HCT 116 C88(0.79)  LDD0636  [2]
 LDCM0323  AC6 HCT 116 C88(1.15)  LDD0640  [2]
 LDCM0332  AC68 HCT 116 C88(1.07)  LDD0649  [2]
 LDCM0333  AC69 HCT 116 C88(0.87)  LDD0650  [2]
 LDCM0334  AC7 HCT 116 C88(1.00)  LDD0651  [2]
 LDCM0335  AC70 HCT 116 C88(1.30)  LDD0652  [2]
 LDCM0336  AC71 HCT 116 C88(0.89)  LDD0653  [2]
 LDCM0337  AC72 HCT 116 C88(1.21)  LDD0654  [2]
 LDCM0338  AC73 HCT 116 C88(1.21)  LDD0655  [2]
 LDCM0339  AC74 HCT 116 C88(1.05)  LDD0656  [2]
 LDCM0340  AC75 HCT 116 C88(1.26)  LDD0657  [2]
 LDCM0341  AC76 HCT 116 C88(0.92)  LDD0658  [2]
 LDCM0342  AC77 HCT 116 C88(0.99)  LDD0659  [2]
 LDCM0343  AC78 HCT 116 C88(1.01)  LDD0660  [2]
 LDCM0344  AC79 HCT 116 C88(1.14)  LDD0661  [2]
 LDCM0345  AC8 HCT 116 C88(0.82)  LDD0662  [2]
 LDCM0346  AC80 HCT 116 C88(0.80)  LDD0663  [2]
 LDCM0347  AC81 HCT 116 C88(1.12)  LDD0664  [2]
 LDCM0348  AC82 HCT 116 C88(0.97)  LDD0665  [2]
 LDCM0365  AC98 PaTu 8988t C88(1.01)  LDD1244  [2]
 LDCM0366  AC99 PaTu 8988t C88(0.78)  LDD1245  [2]
 LDCM0248  AKOS034007472 HCT 116 C88(1.10)  LDD0565  [2]
 LDCM0356  AKOS034007680 HCT 116 C88(1.63)  LDD0673  [2]
 LDCM0275  AKOS034007705 HCT 116 C88(1.70)  LDD0592  [2]
 LDCM0369  CL100 HCT 116 C88(1.22)  LDD0686  [2]
 LDCM0370  CL101 HCT 116 C88(1.33)  LDD0687  [2]
 LDCM0371  CL102 HCT 116 C88(1.29)  LDD0688  [2]
 LDCM0372  CL103 HCT 116 C88(0.99)  LDD0689  [2]
 LDCM0373  CL104 HCT 116 C88(1.32)  LDD0690  [2]
 LDCM0374  CL105 HCT 116 C88(0.75)  LDD0691  [2]
 LDCM0375  CL106 HCT 116 C88(0.74)  LDD0692  [2]
 LDCM0376  CL107 HCT 116 C88(0.77)  LDD0693  [2]
 LDCM0377  CL108 HCT 116 C88(0.79)  LDD0694  [2]
 LDCM0378  CL109 HCT 116 C88(0.81)  LDD0695  [2]
 LDCM0380  CL110 HCT 116 C88(0.96)  LDD0697  [2]
 LDCM0381  CL111 HCT 116 C88(0.87)  LDD0698  [2]
 LDCM0382  CL112 HCT 116 C88(1.11)  LDD0699  [2]
 LDCM0383  CL113 HCT 116 C88(1.34)  LDD0700  [2]
 LDCM0384  CL114 HCT 116 C88(1.43)  LDD0701  [2]
 LDCM0385  CL115 HCT 116 C88(1.77)  LDD0702  [2]
 LDCM0386  CL116 HCT 116 C88(1.29)  LDD0703  [2]
 LDCM0392  CL121 HCT 116 C88(0.84)  LDD0709  [2]
 LDCM0393  CL122 HCT 116 C88(1.07)  LDD0710  [2]
 LDCM0394  CL123 HCT 116 C88(0.85)  LDD0711  [2]
 LDCM0395  CL124 HCT 116 C88(0.69)  LDD0712  [2]
 LDCM0404  CL17 HCT 116 C88(0.88)  LDD0721  [2]
 LDCM0405  CL18 HCT 116 C88(0.85)  LDD0722  [2]
 LDCM0406  CL19 HCT 116 C88(0.92)  LDD0723  [2]
 LDCM0408  CL20 HCT 116 C88(0.97)  LDD0725  [2]
 LDCM0409  CL21 HCT 116 C88(0.83)  LDD0726  [2]
 LDCM0410  CL22 HCT 116 C88(1.19)  LDD0727  [2]
 LDCM0411  CL23 HCT 116 C88(1.03)  LDD0728  [2]
 LDCM0412  CL24 HCT 116 C88(0.88)  LDD0729  [2]
 LDCM0413  CL25 HCT 116 C88(0.79)  LDD0730  [2]
 LDCM0414  CL26 HCT 116 C88(0.83)  LDD0731  [2]
 LDCM0415  CL27 HCT 116 C88(0.94)  LDD0732  [2]
 LDCM0416  CL28 HCT 116 C88(0.71)  LDD0733  [2]
 LDCM0417  CL29 HCT 116 C88(1.03)  LDD0734  [2]
 LDCM0419  CL30 HCT 116 C88(0.88)  LDD0736  [2]
 LDCM0420  CL31 HCT 116 C88(0.84)  LDD0737  [2]
 LDCM0421  CL32 HCT 116 C88(0.84)  LDD0738  [2]
 LDCM0422  CL33 HCT 116 C88(0.89)  LDD0739  [2]
 LDCM0423  CL34 HCT 116 C88(1.02)  LDD0740  [2]
 LDCM0424  CL35 HCT 116 C88(1.16)  LDD0741  [2]
 LDCM0425  CL36 HCT 116 C88(0.95)  LDD0742  [2]
 LDCM0426  CL37 HCT 116 C88(1.15)  LDD0743  [2]
 LDCM0428  CL39 HCT 116 C88(0.87)  LDD0745  [2]
 LDCM0430  CL40 HCT 116 C88(0.87)  LDD0747  [2]
 LDCM0431  CL41 HCT 116 C88(0.85)  LDD0748  [2]
 LDCM0432  CL42 HCT 116 C88(1.04)  LDD0749  [2]
 LDCM0433  CL43 HCT 116 C88(0.75)  LDD0750  [2]
 LDCM0434  CL44 HCT 116 C88(0.86)  LDD0751  [2]
 LDCM0435  CL45 HCT 116 C88(0.66)  LDD0752  [2]
 LDCM0436  CL46 HCT 116 C88(0.78)  LDD0753  [2]
 LDCM0437  CL47 HCT 116 C88(0.93)  LDD0754  [2]
 LDCM0438  CL48 HCT 116 C88(0.89)  LDD0755  [2]
 LDCM0439  CL49 HCT 116 C88(0.65)  LDD0756  [2]
 LDCM0441  CL50 HCT 116 C88(0.74)  LDD0758  [2]
 LDCM0442  CL51 HCT 116 C88(0.67)  LDD0759  [2]
 LDCM0443  CL52 HCT 116 C88(0.86)  LDD0760  [2]
 LDCM0444  CL53 HCT 116 C88(1.50)  LDD0761  [2]
 LDCM0445  CL54 HCT 116 C88(0.62)  LDD0762  [2]
 LDCM0446  CL55 HCT 116 C88(0.89)  LDD0763  [2]
 LDCM0447  CL56 HCT 116 C88(1.03)  LDD0764  [2]
 LDCM0448  CL57 HCT 116 C88(1.11)  LDD0765  [2]
 LDCM0449  CL58 HCT 116 C88(0.71)  LDD0766  [2]
 LDCM0450  CL59 HCT 116 C88(0.78)  LDD0767  [2]
 LDCM0452  CL60 HCT 116 C88(0.65)  LDD0769  [2]
 LDCM0453  CL61 HCT 116 C88(1.20)  LDD0770  [2]
 LDCM0454  CL62 HCT 116 C88(1.06)  LDD0771  [2]
 LDCM0455  CL63 HCT 116 C88(1.26)  LDD0772  [2]
 LDCM0456  CL64 HCT 116 C88(1.17)  LDD0773  [2]
 LDCM0457  CL65 HCT 116 C88(1.21)  LDD0774  [2]
 LDCM0458  CL66 HCT 116 C88(1.09)  LDD0775  [2]
 LDCM0459  CL67 HCT 116 C88(1.08)  LDD0776  [2]
 LDCM0460  CL68 HCT 116 C88(1.37)  LDD0777  [2]
 LDCM0461  CL69 HCT 116 C88(1.20)  LDD0778  [2]
 LDCM0463  CL70 HCT 116 C88(1.11)  LDD0780  [2]
 LDCM0464  CL71 HCT 116 C88(1.34)  LDD0781  [2]
 LDCM0465  CL72 HCT 116 C88(1.26)  LDD0782  [2]
 LDCM0466  CL73 HCT 116 C88(1.21)  LDD0783  [2]
 LDCM0467  CL74 HCT 116 C88(1.36)  LDD0784  [2]
 LDCM0469  CL76 HCT 116 C88(1.08)  LDD0786  [2]
 LDCM0470  CL77 HCT 116 C88(1.00)  LDD0787  [2]
 LDCM0471  CL78 HCT 116 C88(1.13)  LDD0788  [2]
 LDCM0472  CL79 HCT 116 C88(1.53)  LDD0789  [2]
 LDCM0474  CL80 HCT 116 C88(1.19)  LDD0791  [2]
 LDCM0475  CL81 HCT 116 C88(1.09)  LDD0792  [2]
 LDCM0476  CL82 HCT 116 C88(1.32)  LDD0793  [2]
 LDCM0477  CL83 HCT 116 C88(1.13)  LDD0794  [2]
 LDCM0478  CL84 HCT 116 C88(1.45)  LDD0795  [2]
 LDCM0479  CL85 HCT 116 C88(1.11)  LDD0796  [2]
 LDCM0480  CL86 HCT 116 C88(1.03)  LDD0797  [2]
 LDCM0481  CL87 HCT 116 C88(1.07)  LDD0798  [2]
 LDCM0482  CL88 HCT 116 C88(1.19)  LDD0799  [2]
 LDCM0483  CL89 HCT 116 C88(1.63)  LDD0800  [2]
 LDCM0485  CL90 HCT 116 C88(0.95)  LDD0802  [2]
 LDCM0486  CL91 HCT 116 C88(1.04)  LDD0803  [2]
 LDCM0487  CL92 HCT 116 C88(1.05)  LDD0804  [2]
 LDCM0488  CL93 HCT 116 C88(1.03)  LDD0805  [2]
 LDCM0489  CL94 HCT 116 C88(1.10)  LDD0806  [2]
 LDCM0490  CL95 HCT 116 C88(1.19)  LDD0807  [2]
 LDCM0491  CL96 HCT 116 C88(1.16)  LDD0808  [2]
 LDCM0492  CL97 HCT 116 C88(1.14)  LDD0809  [2]
 LDCM0493  CL98 HCT 116 C88(1.12)  LDD0810  [2]
 LDCM0494  CL99 HCT 116 C88(1.21)  LDD0811  [2]
 LDCM0468  Fragment33 HCT 116 C88(1.27)  LDD0785  [2]
 LDCM0427  Fragment51 HCT 116 C88(0.93)  LDD0744  [2]
 LDCM0116  HHS-0101 DM93 Y82(2.45)  LDD0264  [1]
 LDCM0117  HHS-0201 DM93 Y82(0.83)  LDD0265  [1]
 LDCM0118  HHS-0301 DM93 Y82(1.07)  LDD0266  [1]
 LDCM0022  KB02 HCT 116 C88(1.12)  LDD0080  [2]
 LDCM0023  KB03 HCT 116 C88(1.35)  LDD0081  [2]
 LDCM0024  KB05 HCT 116 C88(1.25)  LDD0082  [2]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
E3 ubiquitin-protein ligase RAD18 (RAD18) RAD18 family Q9NS91
E3 ubiquitin-protein ligase UBR1 (UBR1) UBR1 family Q8IWV7
Other
Click To Hide/Show 3 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
DNA damage-inducible transcript 4-like protein (DDIT4L) DDIT4 family Q96D03
Histone H2A type 3 (H2AC25) Histone H2A family Q7L7L0
Homocysteine-responsive endoplasmic reticulum-resident ubiquitin-like domain member 2 protein (HERPUD2) . Q9BSE4

References

1 Discovery of a Cell-Active SuTEx Ligand of Prostaglandin Reductase 2. Chembiochem. 2021 Jun 15;22(12):2134-2139. doi: 10.1002/cbic.202000879. Epub 2021 Apr 29.
2 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
3 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
4 Sequence-Based Prediction of Cysteine Reactivity Using Machine Learning. Biochemistry. 2018 Jan 30;57(4):451-460. doi: 10.1021/acs.biochem.7b00897. Epub 2017 Oct 26.
5 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
6 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.