Details of the Target

General Information of Target

Target ID LDTP05013
Target Name Small ribosomal subunit protein eS25 (RPS25)
Gene Name RPS25
Gene ID 6230
Synonyms
Small ribosomal subunit protein eS25; 40S ribosomal protein S25
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MPPKDDKKKKDAGKSAKKDKDPVNKSGGKAKKKKWSKGKVRDKLNNLVLFDKATYDKLCK
EVPNYKLITPAVVSERLKIRGSLARAALQELLSKGLIKLVSKHRAQVIYTRNTKGGDAPA
AGEDA
Target Bioclass
Other
Family
Eukaryotic ribosomal protein eS25 family
Subcellular location
Cytoplasm
Function Component of the small ribosomal subunit. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell.
Uniprot ID
P62851
Ensemble ID
ENST00000527673.2
HGNC ID
HGNC:10413

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 38 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
m-APA
 Probe Info  		9.12  LDD0402  [1]
A-EBA
 Probe Info  		3.35  LDD0215  [2]
CY4
 Probe Info  		8.86  LDD0244  [3]
N1
 Probe Info  		5.92  LDD0242  [3]
TH211
 Probe Info  		Y65(16.78)  LDD0257  [4]
TH216
 Probe Info  		Y109(12.60); Y65(11.28)  LDD0259  [4]
YN-1
 Probe Info  		100.00  LDD0444  [5]
BTD
 Probe Info  		C59(4.20)  LDD1699  [6]
AZ-9
 Probe Info  		E90(0.98)  LDD2208  [7]
ONAyne
 Probe Info  		K66(0.00); K114(0.00)  LDD0273  [8]
OPA-S-S-alkyne
 Probe Info  		K114(2.69); K66(2.69)  LDD3494  [9]
AMP probe
 Probe Info  		K43(0.00); K52(0.00); K94(0.00)  LDD0200  [10]
ATP probe
 Probe Info  		K43(0.00); K52(0.00); K94(0.00); K66(0.00)  LDD0199  [10]
4-Iodoacetamidophenylacetylene
 Probe Info  		N.A.  LDD0038  [11]
IA-alkyne
 Probe Info  		N.A.  LDD0032  [12]
Lodoacetamide azide
 Probe Info  		N.A.  LDD0037  [11]
ATP probe
 Probe Info  		K114(0.00); K94(0.00)  LDD0035  [13]
IPM
 Probe Info  		N.A.  LDD0025  [14]
JW-RF-010
 Probe Info  		N.A.  LDD0026  [14]
NAIA_4
 Probe Info  		N.A.  LDD2226  [15]
TFBX
 Probe Info  		N.A.  LDD0027  [14]
Compound 10
 Probe Info  		N.A.  LDD2216  [16]
DBIA
 Probe Info  		C59(0.18)  LDD2236  [17]
NHS
 Probe Info  		K66(0.00); K52(0.00)  LDD0010  [18]
OSF
 Probe Info  		N.A.  LDD0029  [19]
SF
 Probe Info  		K102(0.00); Y109(0.00); K18(0.00); K94(0.00)  LDD0028  [19]
STPyne
 Probe Info  		K52(0.00); K66(0.00)  LDD0009  [18]
Phosphinate-6
 Probe Info  		N.A.  LDD0018  [20]
Ox-W18
 Probe Info  		N.A.  LDD2175  [21]
1c-yne
 Probe Info  		K66(0.00); K52(0.00); K43(0.00)  LDD0228  [22]
Acrolein
 Probe Info  		N.A.  LDD0217  [23]
Methacrolein
 Probe Info  		N.A.  LDD0218  [23]
W1
 Probe Info  		N.A.  LDD0236  [24]
AOyne
 Probe Info  		12.80  LDD0443  [25]
NAIA_5
 Probe Info  		N.A.  LDD2223  [15]
TER-AC
 Probe Info  		N.A.  LDD0426  [26]
TPP-AC
 Probe Info  		N.A.  LDD0427  [26]
HHS-482
 Probe Info  		Y109(1.10)  LDD2239  [27]
PAL-AfBPP Probe
Click To Hide/Show 2 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
STS-1
 Probe Info  		1.14  LDD0137  [28]
STS-2
 Probe Info  		N.A.  LDD0138  [28]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0502  1-(Cyanoacetyl)piperidine MDA-MB-231 C59(0.72)  LDD2095  [6]
 LDCM0537  2-Cyano-N,N-dimethylacetamide MDA-MB-231 C59(1.00)  LDD2130  [6]
 LDCM0524  2-Cyano-N-(2-morpholin-4-yl-ethyl)-acetamide MDA-MB-231 C59(0.92)  LDD2117  [6]
 LDCM0558  2-Cyano-N-phenylacetamide MDA-MB-231 C59(1.39)  LDD2152  [6]
 LDCM0510  3-(4-(Hydroxydiphenylmethyl)piperidin-1-yl)-3-oxopropanenitrile MDA-MB-231 C59(1.06)  LDD2103  [6]
 LDCM0539  3-(4-Isopropylpiperazin-1-yl)-3-oxopropanenitrile MDA-MB-231 C59(0.48)  LDD2132  [6]
 LDCM0538  4-(Cyanoacetyl)morpholine MDA-MB-231 C59(0.77)  LDD2131  [6]
 LDCM0545  Acetamide MDA-MB-231 C59(0.46)  LDD2138  [6]
 LDCM0520  AKOS000195272 MDA-MB-231 C59(0.82)  LDD2113  [6]
 LDCM0156  Aniline NCI-H1299 11.63  LDD0403  [1]
 LDCM0498  BS-3668 MDA-MB-231 C59(0.42)  LDD2091  [6]
 LDCM0108  Chloroacetamide HeLa N.A.  LDD0222  [23]
 LDCM0632  CL-Sc Hep-G2 C59(1.06); C59(0.90); C59(0.88); C59(0.51)  LDD2227  [15]
 LDCM0625  F8 Ramos C59(1.33)  LDD2187  [29]
 LDCM0572  Fragment10 Ramos C59(0.79)  LDD2189  [29]
 LDCM0573  Fragment11 Ramos C59(0.00)  LDD2190  [29]
 LDCM0574  Fragment12 Ramos C59(0.81)  LDD2191  [29]
 LDCM0575  Fragment13 Ramos C59(0.85)  LDD2192  [29]
 LDCM0576  Fragment14 Ramos C59(0.76)  LDD2193  [29]
 LDCM0579  Fragment20 Ramos C59(0.57)  LDD2194  [29]
 LDCM0580  Fragment21 Ramos C59(1.00)  LDD2195  [29]
 LDCM0582  Fragment23 Ramos C59(1.84)  LDD2196  [29]
 LDCM0578  Fragment27 Ramos C59(1.15)  LDD2197  [29]
 LDCM0586  Fragment28 Ramos C59(0.92)  LDD2198  [29]
 LDCM0588  Fragment30 Ramos C59(0.82)  LDD2199  [29]
 LDCM0589  Fragment31 Ramos C59(0.90)  LDD2200  [29]
 LDCM0590  Fragment32 Ramos C59(0.64)  LDD2201  [29]
 LDCM0468  Fragment33 Ramos C59(0.72)  LDD2202  [29]
 LDCM0596  Fragment38 Ramos C59(0.82)  LDD2203  [29]
 LDCM0566  Fragment4 Ramos C59(0.71)  LDD2184  [29]
 LDCM0610  Fragment52 Ramos C59(0.68)  LDD2204  [29]
 LDCM0614  Fragment56 Ramos C59(0.79)  LDD2205  [29]
 LDCM0569  Fragment7 Ramos C59(0.62)  LDD2186  [29]
 LDCM0571  Fragment9 Ramos C59(0.56)  LDD2188  [29]
 LDCM0022  KB02 Ramos C59(0.53)  LDD2182  [29]
 LDCM0023  KB03 Ramos C59(0.69)  LDD2183  [29]
 LDCM0024  KB05 Ramos C59(1.36)  LDD2185  [29]
 LDCM0509  N-(4-bromo-3,5-dimethylphenyl)-2-nitroacetamide MDA-MB-231 C59(1.39)  LDD2102  [6]
 LDCM0496  Nucleophilic fragment 11a MDA-MB-231 C59(0.61)  LDD2089  [6]
 LDCM0497  Nucleophilic fragment 11b MDA-MB-231 C59(1.23)  LDD2090  [6]
 LDCM0499  Nucleophilic fragment 12b MDA-MB-231 C59(1.60)  LDD2092  [6]
 LDCM0500  Nucleophilic fragment 13a MDA-MB-231 C59(1.25)  LDD2093  [6]
 LDCM0501  Nucleophilic fragment 13b MDA-MB-231 C59(2.43)  LDD2094  [6]
 LDCM0503  Nucleophilic fragment 14b MDA-MB-231 C59(0.43)  LDD2096  [6]
 LDCM0504  Nucleophilic fragment 15a MDA-MB-231 C59(0.84)  LDD2097  [6]
 LDCM0505  Nucleophilic fragment 15b MDA-MB-231 C59(0.22)  LDD2098  [6]
 LDCM0506  Nucleophilic fragment 16a MDA-MB-231 C59(0.88)  LDD2099  [6]
 LDCM0507  Nucleophilic fragment 16b MDA-MB-231 C59(0.53)  LDD2100  [6]
 LDCM0508  Nucleophilic fragment 17a MDA-MB-231 C59(0.83)  LDD2101  [6]
 LDCM0512  Nucleophilic fragment 19a MDA-MB-231 C59(1.43)  LDD2105  [6]
 LDCM0514  Nucleophilic fragment 20a MDA-MB-231 C59(1.01)  LDD2107  [6]
 LDCM0515  Nucleophilic fragment 20b MDA-MB-231 C59(0.14)  LDD2108  [6]
 LDCM0516  Nucleophilic fragment 21a MDA-MB-231 C59(0.57)  LDD2109  [6]
 LDCM0518  Nucleophilic fragment 22a MDA-MB-231 C59(1.01)  LDD2111  [6]
 LDCM0523  Nucleophilic fragment 24b MDA-MB-231 C59(0.91)  LDD2116  [6]
 LDCM0525  Nucleophilic fragment 25b MDA-MB-231 C59(0.72)  LDD2118  [6]
 LDCM0526  Nucleophilic fragment 26a MDA-MB-231 C59(2.73)  LDD2119  [6]
 LDCM0527  Nucleophilic fragment 26b MDA-MB-231 C59(0.95)  LDD2120  [6]
 LDCM0529  Nucleophilic fragment 27b MDA-MB-231 C59(0.79)  LDD2122  [6]
 LDCM0530  Nucleophilic fragment 28a MDA-MB-231 C59(0.57)  LDD2123  [6]
 LDCM0532  Nucleophilic fragment 29a MDA-MB-231 C59(0.74)  LDD2125  [6]
 LDCM0533  Nucleophilic fragment 29b MDA-MB-231 C59(0.62)  LDD2126  [6]
 LDCM0534  Nucleophilic fragment 30a MDA-MB-231 C59(0.87)  LDD2127  [6]
 LDCM0535  Nucleophilic fragment 30b MDA-MB-231 C59(1.00)  LDD2128  [6]
 LDCM0536  Nucleophilic fragment 31 MDA-MB-231 C59(1.15)  LDD2129  [6]
 LDCM0540  Nucleophilic fragment 35 MDA-MB-231 C59(0.62)  LDD2133  [6]
 LDCM0541  Nucleophilic fragment 36 MDA-MB-231 C59(0.46)  LDD2134  [6]
 LDCM0542  Nucleophilic fragment 37 MDA-MB-231 C59(0.98)  LDD2135  [6]
 LDCM0543  Nucleophilic fragment 38 MDA-MB-231 C59(1.05)  LDD2136  [6]
 LDCM0544  Nucleophilic fragment 39 MDA-MB-231 C59(0.83)  LDD2137  [6]
 LDCM0211  Nucleophilic fragment 3b MDA-MB-231 C59(2.05)  LDD1700  [6]
 LDCM0546  Nucleophilic fragment 40 MDA-MB-231 C59(0.72)  LDD2140  [6]
 LDCM0549  Nucleophilic fragment 43 MDA-MB-231 C59(0.97)  LDD2143  [6]
 LDCM0550  Nucleophilic fragment 5a MDA-MB-231 C59(3.28)  LDD2144  [6]
 LDCM0552  Nucleophilic fragment 6a MDA-MB-231 C59(0.76)  LDD2146  [6]
 LDCM0553  Nucleophilic fragment 6b MDA-MB-231 C59(3.20)  LDD2147  [6]
 LDCM0554  Nucleophilic fragment 7a MDA-MB-231 C59(0.79)  LDD2148  [6]
 LDCM0555  Nucleophilic fragment 7b MDA-MB-231 C59(0.68)  LDD2149  [6]
 LDCM0556  Nucleophilic fragment 8a MDA-MB-231 C59(0.39)  LDD2150  [6]
 LDCM0557  Nucleophilic fragment 8b MDA-MB-231 C59(0.78)  LDD2151  [6]
 LDCM0559  Nucleophilic fragment 9b MDA-MB-231 C59(1.10)  LDD2153  [6]
 LDCM0131  RA190 MM1.R C59(1.39)  LDD0304  [30]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Transcription factor
Click To Hide/Show 3 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Zinc finger and BTB domain-containing protein 14 (ZBTB14) Krueppel C2H2-type zinc-finger protein family O43829
THAP domain-containing protein 1 (THAP1) THAP1 family Q9NVV9
Homeobox-containing protein 1 (HMBOX1) . Q6NT76
Other
Click To Hide/Show 3 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Testis-specific Y-encoded-like protein 2 (TSPYL2) Nucleosome assembly protein (NAP) family Q9H2G4
SH3 and cysteine-rich domain-containing protein 3 (STAC3) . Q96MF2
Uncharacterized protein NKAPD1 (NKAPD1) . Q6ZUT1

References

1 Quantitative and Site-Specific Chemoproteomic Profiling of Targets of Acrolein. Chem Res Toxicol. 2019 Mar 18;32(3):467-473. doi: 10.1021/acs.chemrestox.8b00343. Epub 2019 Jan 15.
2 2-Ethynylbenzaldehyde-Based, Lysine-Targeting Irreversible Covalent Inhibitors for Protein Kinases and Nonkinases. J Am Chem Soc. 2023 Feb 12. doi: 10.1021/jacs.2c11595. Online ahead of print.
Mass spectrometry data entry: PXD037665
3 Cyclopropenone, Cyclopropeniminium Ion, and Cyclopropenethione as Novel Electrophilic Warheads for Potential Target Discovery of Triple-Negative Breast Cancer. J Med Chem. 2023 Feb 23;66(4):2851-2864. doi: 10.1021/acs.jmedchem.2c01889. Epub 2023 Feb 10.
4 Chemoproteomic profiling of kinases in live cells using electrophilic sulfonyl triazole probes. Chem Sci. 2021 Jan 21;12(9):3295-3307. doi: 10.1039/d0sc06623k.
5 Ynamide Electrophile for the Profiling of Ligandable Carboxyl Residues in Live Cells and the Development of New Covalent Inhibitors. J Med Chem. 2022 Aug 11;65(15):10408-10418. doi: 10.1021/acs.jmedchem.2c00272. Epub 2022 Jul 26.
6 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
7 2H-Azirine-Based Reagents for Chemoselective Bioconjugation at Carboxyl Residues Inside Live Cells. J Am Chem Soc. 2020 Apr 1;142(13):6051-6059. doi: 10.1021/jacs.9b12116. Epub 2020 Mar 23.
8 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
9 A chemical proteomics approach for global mapping of functional lysines on cell surface of living cell. Nat Commun. 2024 Apr 8;15(1):2997. doi: 10.1038/s41467-024-47033-w.
Mass spectrometry data entry: PXD042888
10 Targeted Proteomic Approaches for Proteome-Wide Characterizations of the AMP-Binding Capacities of Kinases. J Proteome Res. 2022 Aug 5;21(8):2063-2070. doi: 10.1021/acs.jproteome.2c00225. Epub 2022 Jul 12.
11 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
12 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
13 Comparison of Quantitative Mass Spectrometry Platforms for Monitoring Kinase ATP Probe Uptake in Lung Cancer. J Proteome Res. 2018 Jan 5;17(1):63-75. doi: 10.1021/acs.jproteome.7b00329. Epub 2017 Nov 22.
Mass spectrometry data entry: PXD006095 , PXD006096
14 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
15 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
16 Multiplexed CuAAC Suzuki-Miyaura Labeling for Tandem Activity-Based Chemoproteomic Profiling. Anal Chem. 2021 Feb 2;93(4):2610-2618. doi: 10.1021/acs.analchem.0c04726. Epub 2021 Jan 20.
Mass spectrometry data entry: PXD022279
17 Global profiling of phosphorylation-dependent changes in cysteine reactivity. Nat Methods. 2022 Mar;19(3):341-352. doi: 10.1038/s41592-022-01398-2. Epub 2022 Feb 28.
Mass spectrometry data entry: PXD026730
18 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
19 Solid Phase Synthesis of Fluorosulfate Containing Macrocycles for Chemoproteomic Workflows. bioRxiv [Preprint]. 2023 Feb 18:2023.02.17.529022. doi: 10.1101/2023.02.17.529022.
Mass spectrometry data entry: PXD039931
20 DFT-Guided Discovery of Ethynyl-Triazolyl-Phosphinates as Modular Electrophiles for Chemoselective Cysteine Bioconjugation and Profiling. Angew Chem Int Ed Engl. 2022 Oct 10;61(41):e202205348. doi: 10.1002/anie.202205348. Epub 2022 Aug 22.
Mass spectrometry data entry: PXD033004
21 Oxidative cyclization reagents reveal tryptophan cation- interactions. Nature. 2024 Mar;627(8004):680-687. doi: 10.1038/s41586-024-07140-6. Epub 2024 Mar 6.
Mass spectrometry data entry: PXD001377 , PXD005252
22 Tunable Amine-Reactive Electrophiles for Selective Profiling of Lysine. Angew Chem Int Ed Engl. 2022 Jan 26;61(5):e202112107. doi: 10.1002/anie.202112107. Epub 2021 Dec 16.
23 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
24 Oxidant-Induced Bioconjugation for Protein Labeling in Live Cells. ACS Chem Biol. 2023 Jan 20;18(1):112-122. doi: 10.1021/acschembio.2c00740. Epub 2022 Dec 21.
25 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
26 Differently Tagged Probes for Protein Profiling of Mitochondria. Chembiochem. 2019 May 2;20(9):1155-1160. doi: 10.1002/cbic.201800735. Epub 2019 Mar 26.
27 Global targeting of functional tyrosines using sulfur-triazole exchange chemistry. Nat Chem Biol. 2020 Feb;16(2):150-159. doi: 10.1038/s41589-019-0404-5. Epub 2019 Nov 25.
28 Design and synthesis of minimalist terminal alkyne-containing diazirine photo-crosslinkers and their incorporation into kinase inhibitors for cell- and tissue-based proteome profiling. Angew Chem Int Ed Engl. 2013 Aug 12;52(33):8551-6. doi: 10.1002/anie.201300683. Epub 2013 Jun 10.
29 Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry. Methods Enzymol. 2023;679:295-322. doi: 10.1016/bs.mie.2022.07.037. Epub 2022 Sep 7.
Mass spectrometry data entry: PXD027578
30 Physical and Functional Analysis of the Putative Rpn13 Inhibitor RA190. Cell Chem Biol. 2020 Nov 19;27(11):1371-1382.e6. doi: 10.1016/j.chembiol.2020.08.007. Epub 2020 Aug 27.