Details of the Target

General Information of Target

Target ID LDTP04911
Target Name Ras-related protein Rap-2b (RAP2B)
Gene Name RAP2B
Gene ID 5912
Synonyms
Ras-related protein Rap-2b; EC 3.6.5.2
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MREYKVVVLGSGGVGKSALTVQFVTGSFIEKYDPTIEDFYRKEIEVDSSPSVLEILDTAG
TEQFASMRDLYIKNGQGFILVYSLVNQQSFQDIKPMRDQIIRVKRYERVPMILVGNKVDL
EGEREVSYGEGKALAEEWSCPFMETSAKNKASVDELFAEIVRQMNYAAQPNGDEGCCSAC
VIL
Target Bioclass
Enzyme
Family
Small GTPase superfamily, Ras family
Subcellular location
Recycling endosome membrane
Function
Small GTP-binding protein which cycles between a GDP-bound inactive and a GTP-bound active form. Involved in EGFR and CHRM3 signaling pathways through stimulation of PLCE1. May play a role in cytoskeletal rearrangements and regulate cell spreading through activation of the effector TNIK. May regulate membrane vesiculation in red blood cells.
Uniprot ID
P61225
Ensemble ID
ENST00000323534.5
HGNC ID
HGNC:9862

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 10 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
m-APA
 Probe Info  		7.27  LDD0402  [1]
JZ128-DTB
 Probe Info  		N.A.  LDD0462  [2]
BTD
 Probe Info  		C140(0.96)  LDD2104  [3]
DBIA
 Probe Info  		C140(1.08)  LDD0593  [4]
4-Iodoacetamidophenylacetylene
 Probe Info  		N.A.  LDD0038  [5]
IA-alkyne
 Probe Info  		N.A.  LDD0036  [5]
Lodoacetamide azide
 Probe Info  		N.A.  LDD0037  [5]
IPM
 Probe Info  		N.A.  LDD2156  [6]
AOyne
 Probe Info  		9.10  LDD0443  [7]
NAIA_5
 Probe Info  		N.A.  LDD2223  [8]
PAL-AfBPP Probe
Click To Hide/Show 3 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C293
 Probe Info  		22.47  LDD1963  [9]
FFF probe11
 Probe Info  		6.66  LDD0472  [10]
FFF probe3
 Probe Info  		8.37  LDD0465  [10]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0519  1-(6-methoxy-3,4-dihydroquinolin-1(2H)-yl)-2-nitroethan-1-one MDA-MB-231 C140(1.48)  LDD2112  [3]
 LDCM0214  AC1 HEK-293T C140(1.14)  LDD1507  [11]
 LDCM0226  AC11 HEK-293T C140(1.06)  LDD1509  [11]
 LDCM0237  AC12 HEK-293T C140(1.01)  LDD1510  [11]
 LDCM0259  AC14 HEK-293T C140(1.05)  LDD1512  [11]
 LDCM0276  AC17 HCT 116 C140(1.08)  LDD0593  [4]
 LDCM0277  AC18 HCT 116 C140(0.59)  LDD0594  [4]
 LDCM0278  AC19 HCT 116 C140(0.81)  LDD0595  [4]
 LDCM0280  AC20 HCT 116 C140(0.93)  LDD0597  [4]
 LDCM0281  AC21 HCT 116 C140(0.77)  LDD0598  [4]
 LDCM0282  AC22 HCT 116 C140(1.00)  LDD0599  [4]
 LDCM0283  AC23 HCT 116 C140(0.89)  LDD0600  [4]
 LDCM0284  AC24 HCT 116 C140(1.04)  LDD0601  [4]
 LDCM0285  AC25 HEK-293T C140(1.03)  LDD1524  [11]
 LDCM0287  AC27 HEK-293T C140(1.05)  LDD1526  [11]
 LDCM0288  AC28 HEK-293T C140(1.02)  LDD1527  [11]
 LDCM0289  AC29 HEK-293T C140(1.09)  LDD1528  [11]
 LDCM0290  AC3 HEK-293T C140(1.05)  LDD1529  [11]
 LDCM0291  AC30 HEK-293T C140(1.01)  LDD1530  [11]
 LDCM0294  AC33 HEK-293T C140(1.08)  LDD1533  [11]
 LDCM0296  AC35 HCT 116 C140(1.95)  LDD0613  [4]
 LDCM0297  AC36 HCT 116 C140(1.82)  LDD0614  [4]
 LDCM0298  AC37 HCT 116 C140(1.45)  LDD0615  [4]
 LDCM0299  AC38 HCT 116 C140(1.74)  LDD0616  [4]
 LDCM0300  AC39 HCT 116 C140(1.26)  LDD0617  [4]
 LDCM0301  AC4 HEK-293T C140(1.09)  LDD1540  [11]
 LDCM0302  AC40 HCT 116 C140(0.78)  LDD0619  [4]
 LDCM0303  AC41 HCT 116 C140(1.30)  LDD0620  [4]
 LDCM0304  AC42 HCT 116 C140(1.02)  LDD0621  [4]
 LDCM0305  AC43 HCT 116 C140(1.32)  LDD0622  [4]
 LDCM0306  AC44 HCT 116 C140(0.98)  LDD0623  [4]
 LDCM0307  AC45 HCT 116 C140(0.77)  LDD0624  [4]
 LDCM0308  AC46 HCT 116 C140(1.32)  LDD0625  [4]
 LDCM0309  AC47 HCT 116 C140(0.85)  LDD0626  [4]
 LDCM0310  AC48 HCT 116 C140(1.28)  LDD0627  [4]
 LDCM0311  AC49 HCT 116 C140(0.43)  LDD0628  [4]
 LDCM0312  AC5 HEK-293T C140(1.08)  LDD1551  [11]
 LDCM0313  AC50 HCT 116 C140(0.46)  LDD0630  [4]
 LDCM0314  AC51 HCT 116 C140(1.34)  LDD0631  [4]
 LDCM0315  AC52 HCT 116 C140(0.89)  LDD0632  [4]
 LDCM0316  AC53 HCT 116 C140(0.58)  LDD0633  [4]
 LDCM0317  AC54 HCT 116 C140(0.57)  LDD0634  [4]
 LDCM0318  AC55 HCT 116 C140(0.44)  LDD0635  [4]
 LDCM0319  AC56 HCT 116 C140(0.35)  LDD0636  [4]
 LDCM0320  AC57 HEK-293T C140(1.00)  LDD1559  [11]
 LDCM0322  AC59 HEK-293T C140(0.95)  LDD1561  [11]
 LDCM0323  AC6 HEK-293T C140(0.85)  LDD1562  [11]
 LDCM0324  AC60 HEK-293T C140(1.04)  LDD1563  [11]
 LDCM0325  AC61 HEK-293T C140(1.02)  LDD1564  [11]
 LDCM0326  AC62 HEK-293T C140(1.05)  LDD1565  [11]
 LDCM0248  AKOS034007472 HEK-293T C140(1.05)  LDD1511  [11]
 LDCM0356  AKOS034007680 HEK-293T C140(1.02)  LDD1570  [11]
 LDCM0156  Aniline NCI-H1299 11.55  LDD0403  [1]
 LDCM0367  CL1 HCT 116 C140(0.72)  LDD0684  [4]
 LDCM0368  CL10 HCT 116 C140(0.56)  LDD0685  [4]
 LDCM0369  CL100 HEK-293T C140(1.03)  LDD1573  [11]
 LDCM0370  CL101 HEK-293T C140(0.95)  LDD1574  [11]
 LDCM0371  CL102 HEK-293T C140(1.10)  LDD1575  [11]
 LDCM0373  CL104 HEK-293T C140(1.10)  LDD1577  [11]
 LDCM0374  CL105 HCT 116 C140(0.71)  LDD0691  [4]
 LDCM0375  CL106 HCT 116 C140(0.48)  LDD0692  [4]
 LDCM0376  CL107 HCT 116 C140(0.51)  LDD0693  [4]
 LDCM0377  CL108 HCT 116 C140(0.42)  LDD0694  [4]
 LDCM0378  CL109 HCT 116 C140(0.68)  LDD0695  [4]
 LDCM0379  CL11 HCT 116 C140(0.52)  LDD0696  [4]
 LDCM0380  CL110 HCT 116 C140(0.53)  LDD0697  [4]
 LDCM0381  CL111 HCT 116 C140(0.84)  LDD0698  [4]
 LDCM0382  CL112 HEK-293T C140(1.06)  LDD1586  [11]
 LDCM0383  CL113 HEK-293T C140(1.00)  LDD1587  [11]
 LDCM0384  CL114 HEK-293T C140(1.10)  LDD1588  [11]
 LDCM0386  CL116 HEK-293T C140(1.11)  LDD1590  [11]
 LDCM0387  CL117 HCT 116 C140(0.50)  LDD0704  [4]
 LDCM0388  CL118 HCT 116 C140(1.03)  LDD0705  [4]
 LDCM0389  CL119 HCT 116 C140(0.88)  LDD0706  [4]
 LDCM0390  CL12 HCT 116 C140(0.68)  LDD0707  [4]
 LDCM0391  CL120 HCT 116 C140(1.36)  LDD0708  [4]
 LDCM0392  CL121 HCT 116 C140(0.33)  LDD0709  [4]
 LDCM0393  CL122 HCT 116 C140(0.95)  LDD0710  [4]
 LDCM0394  CL123 HCT 116 C140(0.52)  LDD0711  [4]
 LDCM0395  CL124 HCT 116 C140(0.47)  LDD0712  [4]
 LDCM0396  CL125 HEK-293T C140(0.98)  LDD1600  [11]
 LDCM0397  CL126 HEK-293T C140(1.09)  LDD1601  [11]
 LDCM0399  CL128 HEK-293T C140(0.98)  LDD1603  [11]
 LDCM0400  CL13 HCT 116 C140(0.70)  LDD0717  [4]
 LDCM0401  CL14 HCT 116 C140(0.86)  LDD0718  [4]
 LDCM0402  CL15 HCT 116 C140(0.81)  LDD0719  [4]
 LDCM0403  CL16 HEK-293T C140(1.12)  LDD1607  [11]
 LDCM0404  CL17 HEK-293T C140(1.24)  LDD1608  [11]
 LDCM0406  CL19 HEK-293T C140(1.10)  LDD1610  [11]
 LDCM0407  CL2 HCT 116 C140(0.67)  LDD0724  [4]
 LDCM0408  CL20 HEK-293T C140(1.15)  LDD1612  [11]
 LDCM0409  CL21 HEK-293T C140(1.30)  LDD1613  [11]
 LDCM0410  CL22 HEK-293T C140(1.06)  LDD1614  [11]
 LDCM0413  CL25 HEK-293T C140(1.17)  LDD1617  [11]
 LDCM0414  CL26 HEK-293T C140(1.00)  LDD1618  [11]
 LDCM0416  CL28 HEK-293T C140(1.22)  LDD1620  [11]
 LDCM0417  CL29 HEK-293T C140(1.08)  LDD1621  [11]
 LDCM0418  CL3 HCT 116 C140(1.06)  LDD0735  [4]
 LDCM0420  CL31 HCT 116 C140(0.66)  LDD0737  [4]
 LDCM0421  CL32 HCT 116 C140(0.86)  LDD0738  [4]
 LDCM0422  CL33 HCT 116 C140(0.72)  LDD0739  [4]
 LDCM0423  CL34 HCT 116 C140(0.28)  LDD0740  [4]
 LDCM0424  CL35 HCT 116 C140(0.29)  LDD0741  [4]
 LDCM0425  CL36 HCT 116 C140(0.33)  LDD0742  [4]
 LDCM0426  CL37 HCT 116 C140(0.25)  LDD0743  [4]
 LDCM0428  CL39 HCT 116 C140(0.32)  LDD0745  [4]
 LDCM0429  CL4 HCT 116 C140(1.07)  LDD0746  [4]
 LDCM0430  CL40 HCT 116 C140(0.33)  LDD0747  [4]
 LDCM0431  CL41 HCT 116 C140(0.42)  LDD0748  [4]
 LDCM0432  CL42 HCT 116 C140(0.20)  LDD0749  [4]
 LDCM0433  CL43 HCT 116 C140(0.25)  LDD0750  [4]
 LDCM0434  CL44 HCT 116 C140(0.42)  LDD0751  [4]
 LDCM0435  CL45 HCT 116 C140(0.29)  LDD0752  [4]
 LDCM0436  CL46 HEK-293T C140(1.04)  LDD1640  [11]
 LDCM0439  CL49 HEK-293T C140(1.20)  LDD1643  [11]
 LDCM0440  CL5 HCT 116 C140(0.89)  LDD0757  [4]
 LDCM0441  CL50 HEK-293T C140(1.05)  LDD1645  [11]
 LDCM0443  CL52 HEK-293T C140(1.07)  LDD1646  [11]
 LDCM0444  CL53 HEK-293T C140(1.42)  LDD1647  [11]
 LDCM0446  CL55 HEK-293T C140(1.20)  LDD1649  [11]
 LDCM0447  CL56 HEK-293T C140(1.22)  LDD1650  [11]
 LDCM0448  CL57 HEK-293T C140(1.45)  LDD1651  [11]
 LDCM0449  CL58 HEK-293T C140(0.98)  LDD1652  [11]
 LDCM0451  CL6 HCT 116 C140(0.74)  LDD0768  [4]
 LDCM0453  CL61 HEK-293T C140(0.93)  LDD1656  [11]
 LDCM0454  CL62 HEK-293T C140(0.96)  LDD1657  [11]
 LDCM0456  CL64 HEK-293T C140(1.21)  LDD1659  [11]
 LDCM0457  CL65 HEK-293T C140(1.00)  LDD1660  [11]
 LDCM0459  CL67 HEK-293T C140(1.04)  LDD1662  [11]
 LDCM0460  CL68 HEK-293T C140(1.18)  LDD1663  [11]
 LDCM0461  CL69 HEK-293T C140(1.21)  LDD1664  [11]
 LDCM0462  CL7 HCT 116 C140(0.84)  LDD0779  [4]
 LDCM0463  CL70 HEK-293T C140(0.95)  LDD1666  [11]
 LDCM0466  CL73 HEK-293T C140(1.31)  LDD1669  [11]
 LDCM0467  CL74 HEK-293T C140(0.93)  LDD1670  [11]
 LDCM0469  CL76 HEK-293T C140(0.98)  LDD1672  [11]
 LDCM0470  CL77 HEK-293T C140(1.29)  LDD1673  [11]
 LDCM0472  CL79 HEK-293T C140(1.14)  LDD1675  [11]
 LDCM0473  CL8 HCT 116 C140(0.66)  LDD0790  [4]
 LDCM0474  CL80 HEK-293T C140(1.02)  LDD1677  [11]
 LDCM0475  CL81 HEK-293T C140(1.12)  LDD1678  [11]
 LDCM0476  CL82 HEK-293T C140(0.93)  LDD1679  [11]
 LDCM0479  CL85 HEK-293T C140(1.02)  LDD1682  [11]
 LDCM0480  CL86 HEK-293T C140(1.01)  LDD1683  [11]
 LDCM0482  CL88 HEK-293T C140(1.23)  LDD1685  [11]
 LDCM0483  CL89 HEK-293T C140(1.24)  LDD1686  [11]
 LDCM0484  CL9 HCT 116 C140(0.65)  LDD0801  [4]
 LDCM0486  CL91 HEK-293T C140(1.02)  LDD1689  [11]
 LDCM0487  CL92 HEK-293T C140(1.30)  LDD1690  [11]
 LDCM0488  CL93 HEK-293T C140(1.31)  LDD1691  [11]
 LDCM0489  CL94 HEK-293T C140(1.18)  LDD1692  [11]
 LDCM0492  CL97 HEK-293T C140(1.25)  LDD1695  [11]
 LDCM0493  CL98 HEK-293T C140(1.03)  LDD1696  [11]
 LDCM0625  F8 Ramos C140(0.69)  LDD2187  [12]
 LDCM0573  Fragment11 Ramos C140(0.44)  LDD2190  [12]
 LDCM0576  Fragment14 Ramos C140(2.04)  LDD2193  [12]
 LDCM0586  Fragment28 Ramos C140(0.63)  LDD2198  [12]
 LDCM0566  Fragment4 Ramos C140(0.54)  LDD2184  [12]
 LDCM0427  Fragment51 HCT 116 C140(0.20)  LDD0744  [4]
 LDCM0569  Fragment7 Ramos C140(0.80)  LDD2186  [12]
 LDCM0179  JZ128 PC-3 N.A.  LDD0462  [2]
 LDCM0022  KB02 HEK-293T C140(0.78)  LDD1492  [11]
 LDCM0023  KB03 HEK-293T C140(0.89)  LDD1497  [11]
 LDCM0024  KB05 HMCB C140(1.47)  LDD3312  [13]
 LDCM0511  Nucleophilic fragment 18b MDA-MB-231 C140(0.96)  LDD2104  [3]
 LDCM0515  Nucleophilic fragment 20b MDA-MB-231 C140(0.85)  LDD2108  [3]
 LDCM0521  Nucleophilic fragment 23b MDA-MB-231 C140(0.81)  LDD2114  [3]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Calcium/calmodulin-dependent protein kinase type II subunit beta (CAMK2B) CAMK Ser/Thr protein kinase family Q13554
Other
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
RUN domain-containing protein 3A (RUNDC3A) RUNDC3 family Q59EK9
Ras association domain-containing protein 5 (RASSF5) . Q8WWW0

References

1 Quantitative and Site-Specific Chemoproteomic Profiling of Targets of Acrolein. Chem Res Toxicol. 2019 Mar 18;32(3):467-473. doi: 10.1021/acs.chemrestox.8b00343. Epub 2019 Jan 15.
2 A Chemoproteomic Strategy for Direct and Proteome-Wide Covalent Inhibitor Target-Site Identification. J Am Chem Soc. 2019 Jan 9;141(1):191-203. doi: 10.1021/jacs.8b07911. Epub 2018 Dec 20.
3 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
4 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
5 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
6 Benchmarking Cleavable Biotin Tags for Peptide-Centric Chemoproteomics. J Proteome Res. 2022 May 6;21(5):1349-1358. doi: 10.1021/acs.jproteome.2c00174. Epub 2022 Apr 25.
Mass spectrometry data entry: PXD031019
7 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
8 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
9 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587
10 Ligand and Target Discovery by Fragment-Based Screening in Human Cells. Cell. 2017 Jan 26;168(3):527-541.e29. doi: 10.1016/j.cell.2016.12.029. Epub 2017 Jan 19.
11 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
12 Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry. Methods Enzymol. 2023;679:295-322. doi: 10.1016/bs.mie.2022.07.037. Epub 2022 Sep 7.
Mass spectrometry data entry: PXD027578
13 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840