Details of the Target

General Information of Target

Target ID LDTP01697
Target Name 3'(2'),5'-bisphosphate nucleotidase 1 (BPNT1)
Gene Name BPNT1
Gene ID 10380
Synonyms
3'(2'),5'-bisphosphate nucleotidase 1; EC 3.1.3.7; 3'-phosphoadenosine 5'-phosphate phosphatase; PAP phosphatase; Bisphosphate 3'-nucleotidase 1; BPntase 1; HsPIP; Inositol-polyphosphate 1-phosphatase; EC 3.1.3.57
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MASSNTVLMRLVASAYSIAQKAGMIVRRVIAEGDLGIVEKTCATDLQTKADRLAQMSICS
SLARKFPKLTIIGEEDLPSEEVDQELIEDSQWEEILKQPCPSQYSAIKEEDLVVWVDPLD
GTKEYTEGLLDNVTVLIGIAYEGKAIAGVINQPYYNYEAGPDAVLGRTIWGVLGLGAFGF
QLKEVPAGKHIITTTRSHSNKLVTDCVAAMNPDAVLRVGGAGNKIIQLIEGKASAYVFAS
PGCKKWDTCAPEVILHAVGGKLTDIHGNVLQYHKDVKHMNSAGVLATLRNYDYYASRVPE
SIKNALVP
Target Bioclass
Enzyme
Family
Inositol monophosphatase superfamily
Function
Phosphatase that converts 3'(2')-phosphoadenosine 5'-phosphate (PAP) to AMP and inositol 1,4-bisphosphate (Ins(1,4)P2) to inositol 4-phosphate. Is also able to hydrolyze adenosine 3'-phosphate 5'-phosphosulfate (PAPS) to adenosine 5'-phosphosulfate (APS). Probably prevents the toxic accumulation of PAP, a compound which inhibits a variety of proteins, including PAPS-utilizing enzymes such as sulfotransferases, and RNA processing enzymes. Could also play a role in inositol recycling and phosphoinositide metabolism. Is not active on 3'-AMP, inositol-1-phosphate and inositol-1,4,5-triphosphate.
Uniprot ID
O95861
Ensemble ID
ENST00000322067.12
HGNC ID
HGNC:1096

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
CAL78 SNV: p.Q84E .
LNCaP clone FGC SNV: p.T44I DBIA    Probe Info 
LS513 SNV: p.I254V DBIA    Probe Info 
MCC13 SNV: p.Q227Ter DBIA    Probe Info 
MFE319 SNV: p.I95T DBIA    Probe Info 
SHP77 SNV: p.V114F .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 29 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
m-APA
 Probe Info  		15.00  LDD0402  [1]
ONAyne
 Probe Info  		K49(1.32)  LDD0274  [2]
STPyne
 Probe Info  		K49(9.09)  LDD0277  [2]
Probe 1
 Probe Info  		Y16(16.01); Y293(44.17)  LDD3495  [3]
DBIA
 Probe Info  		C243(1.33); C59(1.76); C206(1.93); C45(1.99)  LDD3312  [4]
BTD
 Probe Info  		C42(1.32)  LDD1700  [5]
AHL-Pu-1
 Probe Info  		C42(2.11)  LDD0169  [6]
5E-2FA
 Probe Info  		H256(0.00); H266(0.00)  LDD2235  [7]
ATP probe
 Probe Info  		N.A.  LDD0199  [8]
4-Iodoacetamidophenylacetylene
 Probe Info  		C243(0.00); C249(0.00); C59(0.00); C206(0.00)  LDD0038  [9]
IA-alkyne
 Probe Info  		C42(0.00); C206(0.00); C249(0.00); C243(0.00)  LDD0032  [10]
IPIAA_H
 Probe Info  		N.A.  LDD0030  [11]
IPIAA_L
 Probe Info  		N.A.  LDD0031  [11]
Lodoacetamide azide
 Probe Info  		C243(0.00); C249(0.00); C59(0.00); C206(0.00)  LDD0037  [9]
ATP probe
 Probe Info  		N.A.  LDD0035  [12]
JW-RF-010
 Probe Info  		N.A.  LDD0026  [13]
NAIA_4
 Probe Info  		C42(0.00); C206(0.00); C243(0.00)  LDD2226  [14]
WYneO
 Probe Info  		N.A.  LDD0022  [15]
DA-P3
 Probe Info  		N.A.  LDD0178  [16]
Compound 10
 Probe Info  		N.A.  LDD2216  [17]
Compound 11
 Probe Info  		N.A.  LDD2213  [17]
ENE
 Probe Info  		N.A.  LDD0006  [15]
IPM
 Probe Info  		N.A.  LDD0005  [15]
TFBX
 Probe Info  		C206(0.00); C59(0.00); C42(0.00); C243(0.00)  LDD0148  [13]
Phosphinate-6
 Probe Info  		N.A.  LDD0018  [18]
Acrolein
 Probe Info  		C206(0.00); C249(0.00); C59(0.00)  LDD0217  [19]
Methacrolein
 Probe Info  		C206(0.00); C42(0.00)  LDD0218  [19]
AOyne
 Probe Info  		11.10  LDD0443  [20]
NAIA_5
 Probe Info  		C59(0.00); C243(0.00); C249(0.00); C206(0.00)  LDD2223  [14]
PAL-AfBPP Probe
Click To Hide/Show 2 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
STS-2
 Probe Info  		N.A.  LDD0139  [21]
VE-P
 Probe Info  		N.A.  LDD0396  [22]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0548  1-(4-(Benzo[d][1,3]dioxol-5-ylmethyl)piperazin-1-yl)-2-nitroethan-1-one MDA-MB-231 C42(0.79); C59(0.49)  LDD2142  [5]
 LDCM0519  1-(6-methoxy-3,4-dihydroquinolin-1(2H)-yl)-2-nitroethan-1-one MDA-MB-231 C42(0.98)  LDD2112  [5]
 LDCM0502  1-(Cyanoacetyl)piperidine MDA-MB-231 C42(0.78)  LDD2095  [5]
 LDCM0537  2-Cyano-N,N-dimethylacetamide MDA-MB-231 C42(0.96)  LDD2130  [5]
 LDCM0558  2-Cyano-N-phenylacetamide MDA-MB-231 C42(1.47)  LDD2152  [5]
 LDCM0538  4-(Cyanoacetyl)morpholine MDA-MB-231 C42(0.72)  LDD2131  [5]
 LDCM0026  4SU-RNA+native RNA HEK-293T C42(2.11)  LDD0169  [6]
 LDCM0214  AC1 HEK-293T C42(0.85); C100(0.86); C243(0.96); C206(1.03)  LDD1507  [23]
 LDCM0215  AC10 HEK-293T C42(0.99); C100(0.89); C243(0.98); C206(1.00)  LDD1508  [23]
 LDCM0226  AC11 HEK-293T C42(1.00); C100(0.98); C243(0.94); C206(1.00)  LDD1509  [23]
 LDCM0237  AC12 HEK-293T C42(1.04); C100(1.00); C206(1.07); C249(0.92)  LDD1510  [23]
 LDCM0259  AC14 HEK-293T C42(0.97); C100(1.03); C243(1.01); C206(1.01)  LDD1512  [23]
 LDCM0270  AC15 HEK-293T C42(1.00); C100(0.97); C243(0.91); C206(1.02)  LDD1513  [23]
 LDCM0276  AC17 HEK-293T C42(0.90); C100(0.84); C243(0.94); C206(0.98)  LDD1515  [23]
 LDCM0277  AC18 HEK-293T C42(1.02); C100(0.95); C243(1.02); C206(1.02)  LDD1516  [23]
 LDCM0278  AC19 HEK-293T C42(0.94); C100(0.93); C243(0.89); C206(1.00)  LDD1517  [23]
 LDCM0279  AC2 HEK-293T C42(0.96); C100(0.93); C243(0.95); C206(1.10)  LDD1518  [23]
 LDCM0280  AC20 HEK-293T C42(1.04); C100(0.91); C206(0.90); C249(0.95)  LDD1519  [23]
 LDCM0281  AC21 HEK-293T C42(1.08); C100(0.96); C243(0.97); C206(1.16)  LDD1520  [23]
 LDCM0282  AC22 HEK-293T C42(0.97); C100(1.03); C243(1.15); C206(0.94)  LDD1521  [23]
 LDCM0283  AC23 HEK-293T C42(0.97); C100(0.99); C243(0.97); C206(1.02)  LDD1522  [23]
 LDCM0284  AC24 HEK-293T C42(0.98); C100(0.92); C243(0.99); C206(1.01)  LDD1523  [23]
 LDCM0285  AC25 HEK-293T C42(1.00); C100(0.81); C243(0.97); C206(0.97)  LDD1524  [23]
 LDCM0286  AC26 HEK-293T C42(0.98); C100(0.92); C243(0.97); C206(1.06)  LDD1525  [23]
 LDCM0287  AC27 HEK-293T C42(1.03); C100(0.89); C243(0.96); C206(1.03)  LDD1526  [23]
 LDCM0288  AC28 HEK-293T C42(1.03); C100(0.92); C206(0.94); C249(1.00)  LDD1527  [23]
 LDCM0289  AC29 HEK-293T C42(1.01); C100(0.96); C243(1.00); C206(1.31)  LDD1528  [23]
 LDCM0290  AC3 HEK-293T C42(1.01); C100(0.99); C243(0.96); C206(1.08)  LDD1529  [23]
 LDCM0291  AC30 HEK-293T C42(0.95); C100(1.06); C243(0.87); C206(1.13)  LDD1530  [23]
 LDCM0292  AC31 HEK-293T C42(1.01); C100(0.98); C243(1.12); C206(0.95)  LDD1531  [23]
 LDCM0293  AC32 HEK-293T C42(1.03); C100(1.04); C243(0.96); C206(0.87)  LDD1532  [23]
 LDCM0294  AC33 HEK-293T C42(0.89); C100(0.87); C243(0.93); C206(0.96)  LDD1533  [23]
 LDCM0295  AC34 HEK-293T C42(0.90); C100(0.90); C243(0.97); C206(1.00)  LDD1534  [23]
 LDCM0296  AC35 HEK-293T C42(1.00); C100(0.86); C243(0.90); C206(1.07)  LDD1535  [23]
 LDCM0297  AC36 HEK-293T C42(0.97); C100(0.86); C206(1.09); C249(0.98)  LDD1536  [23]
 LDCM0298  AC37 HEK-293T C42(0.98); C100(1.04); C243(0.99); C206(1.06)  LDD1537  [23]
 LDCM0299  AC38 HEK-293T C42(0.98); C100(1.02); C243(1.01); C206(1.02)  LDD1538  [23]
 LDCM0300  AC39 HEK-293T C42(1.02); C100(0.95); C243(1.01); C206(1.01)  LDD1539  [23]
 LDCM0301  AC4 HEK-293T C42(0.99); C100(1.05); C206(1.09); C249(1.05)  LDD1540  [23]
 LDCM0302  AC40 HEK-293T C42(1.02); C100(1.01); C243(0.97); C206(1.04)  LDD1541  [23]
 LDCM0303  AC41 HEK-293T C42(0.83); C100(0.82); C243(0.94); C206(0.99)  LDD1542  [23]
 LDCM0304  AC42 HEK-293T C42(0.95); C100(0.89); C243(0.90); C206(1.05)  LDD1543  [23]
 LDCM0305  AC43 HEK-293T C42(1.02); C100(0.94); C243(0.94); C206(1.07)  LDD1544  [23]
 LDCM0306  AC44 HEK-293T C42(0.97); C100(1.05); C206(1.09); C249(0.90)  LDD1545  [23]
 LDCM0307  AC45 HEK-293T C42(0.97); C100(1.00); C243(1.09); C206(1.13)  LDD1546  [23]
 LDCM0308  AC46 HEK-293T C42(0.96); C100(1.04); C243(0.98); C206(0.91)  LDD1547  [23]
 LDCM0309  AC47 HEK-293T C42(0.98); C100(0.98); C243(1.02); C206(0.98)  LDD1548  [23]
 LDCM0310  AC48 HEK-293T C42(1.02); C100(1.01); C243(1.00); C206(1.05)  LDD1549  [23]
 LDCM0311  AC49 HEK-293T C42(0.89); C100(0.73); C243(0.95); C206(1.01)  LDD1550  [23]
 LDCM0312  AC5 HEK-293T C42(1.02); C100(1.02); C243(1.17); C206(1.02)  LDD1551  [23]
 LDCM0313  AC50 HEK-293T C42(0.97); C100(0.87); C243(0.96); C206(0.98)  LDD1552  [23]
 LDCM0314  AC51 HEK-293T C42(1.05); C100(0.86); C243(0.95); C206(1.03)  LDD1553  [23]
 LDCM0315  AC52 HEK-293T C42(1.02); C100(0.97); C206(0.96); C249(0.93)  LDD1554  [23]
 LDCM0316  AC53 HEK-293T C42(0.97); C100(0.93); C243(1.00); C206(1.00)  LDD1555  [23]
 LDCM0317  AC54 HEK-293T C42(1.03); C100(1.00); C243(1.03); C206(0.96)  LDD1556  [23]
 LDCM0318  AC55 HEK-293T C42(1.01); C100(0.92); C243(0.99); C206(0.97)  LDD1557  [23]
 LDCM0319  AC56 HEK-293T C42(1.01); C100(0.96); C243(0.94); C206(1.07)  LDD1558  [23]
 LDCM0320  AC57 HEK-293T C42(0.85); C100(0.84); C243(0.95); C206(0.94)  LDD1559  [23]
 LDCM0321  AC58 HEK-293T C42(0.94); C100(0.95); C243(0.87); C206(1.03)  LDD1560  [23]
 LDCM0322  AC59 HEK-293T C42(0.96); C100(1.00); C243(0.90); C206(1.11)  LDD1561  [23]
 LDCM0323  AC6 HEK-293T C42(0.96); C100(1.04); C243(0.98); C206(1.06)  LDD1562  [23]
 LDCM0324  AC60 HEK-293T C42(0.96); C100(0.87); C206(0.99); C249(0.92)  LDD1563  [23]
 LDCM0325  AC61 HEK-293T C42(1.01); C100(0.97); C243(1.01); C206(1.12)  LDD1564  [23]
 LDCM0326  AC62 HEK-293T C42(0.97); C100(1.01); C243(0.94); C206(0.99)  LDD1565  [23]
 LDCM0327  AC63 HEK-293T C42(0.98); C100(0.94); C243(0.95); C206(1.00)  LDD1566  [23]
 LDCM0328  AC64 HEK-293T C42(0.98); C100(1.04); C243(1.00); C206(1.05)  LDD1567  [23]
 LDCM0334  AC7 HEK-293T C42(0.99); C100(0.97); C243(1.01); C206(0.99)  LDD1568  [23]
 LDCM0345  AC8 HEK-293T C42(1.04); C100(1.10); C243(0.97); C206(1.06)  LDD1569  [23]
 LDCM0248  AKOS034007472 HEK-293T C42(1.05); C100(0.96); C243(1.00); C206(0.97)  LDD1511  [23]
 LDCM0356  AKOS034007680 HEK-293T C42(0.84); C100(0.83); C243(0.93); C206(1.01)  LDD1570  [23]
 LDCM0275  AKOS034007705 HEK-293T C42(1.03); C100(1.03); C243(1.01); C206(1.11)  LDD1514  [23]
 LDCM0156  Aniline NCI-H1299 12.02  LDD0403  [1]
 LDCM0498  BS-3668 MDA-MB-231 C59(0.75)  LDD2091  [5]
 LDCM0108  Chloroacetamide HeLa C249(0.00); C206(0.00); C59(0.00); H278(0.00)  LDD0222  [19]
 LDCM0632  CL-Sc Hep-G2 C42(20.00); C249(1.69); C206(1.23); C243(0.73)  LDD2227  [14]
 LDCM0367  CL1 HEK-293T C42(0.93); C100(0.95); C243(0.99); C206(1.07)  LDD1571  [23]
 LDCM0368  CL10 HEK-293T C42(1.05); C100(1.13); C243(0.80); C206(0.98)  LDD1572  [23]
 LDCM0369  CL100 HEK-293T C42(0.93); C100(0.96); C243(0.95); C206(1.01)  LDD1573  [23]
 LDCM0370  CL101 HEK-293T C42(0.96); C100(1.01); C243(0.97); C206(0.95)  LDD1574  [23]
 LDCM0371  CL102 HEK-293T C42(0.88); C100(0.76); C243(0.95); C206(0.97)  LDD1575  [23]
 LDCM0372  CL103 HEK-293T C42(1.01); C100(0.84); C243(0.93); C206(1.11)  LDD1576  [23]
 LDCM0373  CL104 HEK-293T C42(0.87); C100(0.99); C243(1.05); C206(0.95)  LDD1577  [23]
 LDCM0374  CL105 HEK-293T C42(0.86); C100(0.95); C243(1.01); C206(0.98)  LDD1578  [23]
 LDCM0375  CL106 HEK-293T C42(0.98); C100(0.98); C243(1.01); C206(0.95)  LDD1579  [23]
 LDCM0376  CL107 HEK-293T C42(1.01); C100(1.07); C243(0.99); C206(1.08)  LDD1580  [23]
 LDCM0377  CL108 HEK-293T C42(0.96); C100(0.94); C243(1.02); C206(0.99)  LDD1581  [23]
 LDCM0378  CL109 HEK-293T C42(0.90); C100(1.00); C243(1.07); C206(1.03)  LDD1582  [23]
 LDCM0379  CL11 HEK-293T C42(1.21); C100(1.34); C243(0.98); C206(0.99)  LDD1583  [23]
 LDCM0380  CL110 HEK-293T C42(0.95); C100(0.99); C243(0.92); C206(0.96)  LDD1584  [23]
 LDCM0381  CL111 HEK-293T C42(1.00); C100(0.91); C243(1.00); C206(1.04)  LDD1585  [23]
 LDCM0382  CL112 HEK-293T C42(1.05); C100(0.96); C243(0.94); C206(0.95)  LDD1586  [23]
 LDCM0383  CL113 HEK-293T C42(0.88); C100(1.01); C243(0.94); C206(1.04)  LDD1587  [23]
 LDCM0384  CL114 HEK-293T C42(0.84); C100(0.92); C243(0.91); C206(1.14)  LDD1588  [23]
 LDCM0385  CL115 HEK-293T C42(1.03); C100(0.84); C243(0.99); C206(0.85)  LDD1589  [23]
 LDCM0386  CL116 HEK-293T C42(1.00); C100(0.99); C243(0.98); C206(1.03)  LDD1590  [23]
 LDCM0387  CL117 HEK-293T C42(0.89); C100(0.99); C243(0.93); C206(0.97)  LDD1591  [23]
 LDCM0388  CL118 HEK-293T C42(0.83); C100(0.95); C243(0.95); C206(1.01)  LDD1592  [23]
 LDCM0389  CL119 HEK-293T C42(1.03); C100(0.88); C243(1.02); C206(1.14)  LDD1593  [23]
 LDCM0390  CL12 HEK-293T C42(1.23); C100(1.11); C243(1.00); C206(1.05)  LDD1594  [23]
 LDCM0391  CL120 HEK-293T C42(0.96); C100(1.02); C243(1.02); C206(1.09)  LDD1595  [23]
 LDCM0392  CL121 HEK-293T C42(0.97); C100(0.92); C243(0.95); C206(1.02)  LDD1596  [23]
 LDCM0393  CL122 HEK-293T C42(0.88); C100(0.84); C243(0.93); C206(0.98)  LDD1597  [23]
 LDCM0394  CL123 HEK-293T C42(0.95); C100(0.86); C243(0.88); C206(1.12)  LDD1598  [23]
 LDCM0395  CL124 HEK-293T C42(0.89); C100(0.84); C243(0.94); C206(0.95)  LDD1599  [23]
 LDCM0396  CL125 HEK-293T C42(0.86); C100(1.01); C243(0.97); C206(0.98)  LDD1600  [23]
 LDCM0397  CL126 HEK-293T C42(0.87); C100(1.02); C243(0.90); C206(1.05)  LDD1601  [23]
 LDCM0398  CL127 HEK-293T C42(0.99); C100(1.08); C243(1.04); C206(1.09)  LDD1602  [23]
 LDCM0399  CL128 HEK-293T C42(0.90); C100(1.07); C243(0.99); C206(0.92)  LDD1603  [23]
 LDCM0400  CL13 HEK-293T C42(0.91); C100(0.90); C243(0.91); C206(0.87)  LDD1604  [23]
 LDCM0401  CL14 HEK-293T C42(0.92); C100(0.93); C243(0.78); C206(1.05)  LDD1605  [23]
 LDCM0402  CL15 HEK-293T C42(0.93); C100(0.81); C243(0.84); C206(1.25)  LDD1606  [23]
 LDCM0403  CL16 HEK-293T C42(1.02); C100(1.05); C243(0.96); C206(1.05)  LDD1607  [23]
 LDCM0404  CL17 HEK-293T C42(0.89); C100(0.90); C243(0.98); C206(1.03)  LDD1608  [23]
 LDCM0405  CL18 HEK-293T C42(1.02); C100(0.91); C243(0.85); C206(1.10)  LDD1609  [23]
 LDCM0406  CL19 HEK-293T C42(1.09); C100(0.98); C243(0.92); C206(1.04)  LDD1610  [23]
 LDCM0407  CL2 HEK-293T C42(0.86); C100(0.90); C243(0.82); C206(1.13)  LDD1611  [23]
 LDCM0408  CL20 HEK-293T C42(1.19); C100(1.30); C206(0.91); C249(0.88)  LDD1612  [23]
 LDCM0409  CL21 HEK-293T C42(1.08); C100(1.20); C243(1.08); C206(1.06)  LDD1613  [23]
 LDCM0410  CL22 HEK-293T C42(1.16); C100(1.29); C243(1.06); C206(1.03)  LDD1614  [23]
 LDCM0411  CL23 HEK-293T C42(1.24); C100(1.36); C243(1.08); C206(0.94)  LDD1615  [23]
 LDCM0412  CL24 HEK-293T C42(1.31); C100(1.14); C243(1.03); C206(1.09)  LDD1616  [23]
 LDCM0413  CL25 HEK-293T C42(0.86); C100(0.97); C243(0.95); C206(0.98)  LDD1617  [23]
 LDCM0414  CL26 HEK-293T C42(0.88); C100(0.98); C243(1.06); C206(1.05)  LDD1618  [23]
 LDCM0415  CL27 HEK-293T C42(1.03); C100(0.90); C243(0.99); C206(1.05)  LDD1619  [23]
 LDCM0416  CL28 HEK-293T C42(0.96); C100(0.93); C243(0.97); C206(1.03)  LDD1620  [23]
 LDCM0417  CL29 HEK-293T C42(0.98); C100(0.86); C243(0.88); C206(1.08)  LDD1621  [23]
 LDCM0418  CL3 HEK-293T C42(1.01); C100(0.93); C243(0.98); C206(1.13)  LDD1622  [23]
 LDCM0419  CL30 HEK-293T C42(0.99); C100(0.96); C243(1.02); C206(0.99)  LDD1623  [23]
 LDCM0420  CL31 HEK-293T C42(1.05); C100(1.12); C243(0.91); C206(1.11)  LDD1624  [23]
 LDCM0421  CL32 HEK-293T C42(1.10); C100(1.10); C206(1.05); C249(0.95)  LDD1625  [23]
 LDCM0422  CL33 HEK-293T C42(0.93); C100(1.22); C243(0.95); C206(1.00)  LDD1626  [23]
 LDCM0423  CL34 HEK-293T C42(1.08); C100(1.24); C243(0.95); C206(1.14)  LDD1627  [23]
 LDCM0424  CL35 HEK-293T C42(1.09); C100(1.37); C243(1.03); C206(1.00)  LDD1628  [23]
 LDCM0425  CL36 HEK-293T C42(1.08); C100(1.09); C243(1.00); C206(1.08)  LDD1629  [23]
 LDCM0426  CL37 HEK-293T C42(0.97); C100(1.03); C243(1.02); C206(1.00)  LDD1630  [23]
 LDCM0428  CL39 HEK-293T C42(0.96); C100(0.86); C243(1.06); C206(1.12)  LDD1632  [23]
 LDCM0429  CL4 HEK-293T C42(0.96); C100(0.97); C243(0.92); C206(1.05)  LDD1633  [23]
 LDCM0430  CL40 HEK-293T C42(0.97); C100(1.10); C243(1.05); C206(1.07)  LDD1634  [23]
 LDCM0431  CL41 HEK-293T C42(0.85); C100(0.90); C243(0.96); C206(0.98)  LDD1635  [23]
 LDCM0432  CL42 HEK-293T C42(1.05); C100(1.10); C243(1.07); C206(1.12)  LDD1636  [23]
 LDCM0433  CL43 HEK-293T C42(1.05); C100(1.13); C243(1.01); C206(1.23)  LDD1637  [23]
 LDCM0434  CL44 HEK-293T C42(1.06); C100(1.13); C206(1.13); C249(0.89)  LDD1638  [23]
 LDCM0435  CL45 HEK-293T C42(1.13); C100(1.19); C243(0.98); C206(1.15)  LDD1639  [23]
 LDCM0436  CL46 HEK-293T C42(1.02); C100(1.29); C243(1.15); C206(1.13)  LDD1640  [23]
 LDCM0437  CL47 HEK-293T C42(1.12); C100(1.38); C243(1.08); C206(1.00)  LDD1641  [23]
 LDCM0438  CL48 HEK-293T C42(1.06); C100(1.17); C243(1.01); C206(1.16)  LDD1642  [23]
 LDCM0439  CL49 HEK-293T C42(0.84); C100(0.95); C243(1.03); C206(1.01)  LDD1643  [23]
 LDCM0440  CL5 HEK-293T C42(1.02); C100(0.97); C243(0.95); C206(1.05)  LDD1644  [23]
 LDCM0441  CL50 HEK-293T C42(0.90); C100(0.95); C243(0.87); C206(0.96)  LDD1645  [23]
 LDCM0443  CL52 HEK-293T C42(0.98); C100(1.00); C243(1.00); C206(1.09)  LDD1646  [23]
 LDCM0444  CL53 HEK-293T C42(0.76); C100(0.94); C243(1.01); C206(1.11)  LDD1647  [23]
 LDCM0445  CL54 HEK-293T C42(0.86); C100(0.94); C243(0.86); C206(1.20)  LDD1648  [23]
 LDCM0446  CL55 HEK-293T C42(0.99); C100(1.18); C243(0.95); C206(1.08)  LDD1649  [23]
 LDCM0447  CL56 HEK-293T C42(1.01); C100(1.29); C206(1.22); C249(0.91)  LDD1650  [23]
 LDCM0448  CL57 HEK-293T C42(0.94); C100(1.20); C243(1.08); C206(1.07)  LDD1651  [23]
 LDCM0449  CL58 HEK-293T C42(1.00); C100(1.34); C243(1.06); C206(1.05)  LDD1652  [23]
 LDCM0450  CL59 HEK-293T C42(1.12); C100(1.36); C243(1.05); C206(0.97)  LDD1653  [23]
 LDCM0451  CL6 HEK-293T C42(0.95); C100(0.96); C243(0.81); C206(1.08)  LDD1654  [23]
 LDCM0452  CL60 HEK-293T C42(1.04); C100(1.18); C243(1.05); C206(1.11)  LDD1655  [23]
 LDCM0453  CL61 HEK-293T C42(0.90); C100(1.01); C243(1.00); C206(0.96)  LDD1656  [23]
 LDCM0454  CL62 HEK-293T C42(0.89); C100(0.90); C243(0.89); C206(1.09)  LDD1657  [23]
 LDCM0455  CL63 HEK-293T C42(1.02); C100(0.94); C243(1.01); C206(1.08)  LDD1658  [23]
 LDCM0456  CL64 HEK-293T C42(0.88); C100(0.99); C243(0.94); C206(1.01)  LDD1659  [23]
 LDCM0457  CL65 HEK-293T C42(0.87); C100(0.88); C243(0.93); C206(1.00)  LDD1660  [23]
 LDCM0458  CL66 HEK-293T C42(0.96); C100(0.96); C243(0.87); C206(1.04)  LDD1661  [23]
 LDCM0459  CL67 HEK-293T C42(1.01); C100(1.10); C243(1.04); C206(1.18)  LDD1662  [23]
 LDCM0460  CL68 HEK-293T C42(0.98); C100(1.25); C206(1.00); C249(0.83)  LDD1663  [23]
 LDCM0461  CL69 HEK-293T C42(1.01); C100(1.13); C243(1.03); C206(1.04)  LDD1664  [23]
 LDCM0462  CL7 HEK-293T C42(1.06); C100(1.07); C243(0.90); C206(1.10)  LDD1665  [23]
 LDCM0463  CL70 HEK-293T C42(1.06); C100(1.26); C243(0.90); C206(1.00)  LDD1666  [23]
 LDCM0464  CL71 HEK-293T C42(1.07); C100(1.23); C243(0.92); C206(0.96)  LDD1667  [23]
 LDCM0465  CL72 HEK-293T C42(1.11); C100(1.10); C243(1.09); C206(1.11)  LDD1668  [23]
 LDCM0466  CL73 HEK-293T C42(0.90); C100(1.08); C243(1.01); C206(0.99)  LDD1669  [23]
 LDCM0467  CL74 HEK-293T C42(0.96); C100(0.97); C243(1.01); C206(1.04)  LDD1670  [23]
 LDCM0469  CL76 HEK-293T C42(0.95); C100(1.02); C243(1.03); C206(0.90)  LDD1672  [23]
 LDCM0470  CL77 HEK-293T C42(0.77); C100(0.81); C243(0.96); C206(1.01)  LDD1673  [23]
 LDCM0471  CL78 HEK-293T C42(0.97); C100(0.98); C243(0.95); C206(1.03)  LDD1674  [23]
 LDCM0472  CL79 HEK-293T C42(1.01); C100(1.09); C243(1.00); C206(1.08)  LDD1675  [23]
 LDCM0473  CL8 HEK-293T C42(0.86); C100(1.08); C206(0.97); C249(0.69)  LDD1676  [23]
 LDCM0474  CL80 HEK-293T C42(0.98); C100(1.31); C206(1.10); C249(1.03)  LDD1677  [23]
 LDCM0475  CL81 HEK-293T C42(1.07); C100(1.16); C243(1.06); C206(1.20)  LDD1678  [23]
 LDCM0476  CL82 HEK-293T C42(1.01); C100(1.25); C243(0.89); C206(1.10)  LDD1679  [23]
 LDCM0477  CL83 HEK-293T C42(1.03); C100(1.28); C243(1.11); C206(0.99)  LDD1680  [23]
 LDCM0478  CL84 HEK-293T C42(1.04); C100(1.16); C243(1.09); C206(1.05)  LDD1681  [23]
 LDCM0479  CL85 HEK-293T C42(0.98); C100(1.04); C243(1.00); C206(1.05)  LDD1682  [23]
 LDCM0480  CL86 HEK-293T C42(0.86); C100(1.03); C243(0.94); C206(1.04)  LDD1683  [23]
 LDCM0481  CL87 HEK-293T C42(1.01); C100(1.12); C243(0.98); C206(1.01)  LDD1684  [23]
 LDCM0482  CL88 HEK-293T C42(0.95); C100(1.15); C243(0.96); C206(0.96)  LDD1685  [23]
 LDCM0483  CL89 HEK-293T C42(0.88); C100(0.89); C243(0.93); C206(1.01)  LDD1686  [23]
 LDCM0484  CL9 HEK-293T C42(1.12); C100(1.22); C243(1.12); C206(1.03)  LDD1687  [23]
 LDCM0485  CL90 HEK-293T C42(0.80); C100(1.00); C243(0.86); C206(1.02)  LDD1688  [23]
 LDCM0486  CL91 HEK-293T C42(0.99); C100(1.19); C243(1.02); C206(1.07)  LDD1689  [23]
 LDCM0487  CL92 HEK-293T C42(0.94); C100(0.96); C206(1.07); C249(0.83)  LDD1690  [23]
 LDCM0488  CL93 HEK-293T C42(0.99); C100(1.14); C243(1.11); C206(1.08)  LDD1691  [23]
 LDCM0489  CL94 HEK-293T C42(1.03); C100(1.17); C243(1.03); C206(1.06)  LDD1692  [23]
 LDCM0490  CL95 HEK-293T C42(0.98); C100(1.25); C243(0.89); C206(0.89)  LDD1693  [23]
 LDCM0491  CL96 HEK-293T C42(1.00); C100(0.99); C243(1.01); C206(1.11)  LDD1694  [23]
 LDCM0492  CL97 HEK-293T C42(0.90); C100(1.00); C243(1.10); C206(1.01)  LDD1695  [23]
 LDCM0493  CL98 HEK-293T C42(0.87); C100(0.87); C243(1.04); C206(1.11)  LDD1696  [23]
 LDCM0494  CL99 HEK-293T C42(1.02); C100(0.94); C243(1.06); C206(1.01)  LDD1697  [23]
 LDCM0634  CY-0357 Hep-G2 C42(15.31); C243(3.12)  LDD2228  [14]
 LDCM0027  Dopamine HEK-293T 8.51  LDD0179  [16]
 LDCM0495  E2913 HEK-293T C42(1.03); C100(0.95); C243(0.97); C206(1.18)  LDD1698  [23]
 LDCM0625  F8 Ramos C42(1.33); C59(0.40); C243(1.18); C206(0.60)  LDD2187  [24]
 LDCM0572  Fragment10 Ramos C42(0.78); C59(0.59); C243(0.66); C206(0.74)  LDD2189  [24]
 LDCM0573  Fragment11 Ramos C42(0.78); C59(0.10); C243(0.18); C206(0.21)  LDD2190  [24]
 LDCM0574  Fragment12 Ramos C42(0.32); C59(0.70); C243(0.67); C206(0.93)  LDD2191  [24]
 LDCM0575  Fragment13 Ramos C42(0.60); C59(1.19); C243(1.02); C206(1.25)  LDD2192  [24]
 LDCM0576  Fragment14 Ramos C42(0.40); C59(0.54); C243(0.67); C206(0.72)  LDD2193  [24]
 LDCM0579  Fragment20 Ramos C42(0.26); C59(0.51); C243(0.48); C206(0.77)  LDD2194  [24]
 LDCM0580  Fragment21 Ramos C42(0.73); C59(1.04); C243(1.01); C206(1.06)  LDD2195  [24]
 LDCM0582  Fragment23 Ramos C42(0.65); C59(1.73); C243(1.01); C206(1.47)  LDD2196  [24]
 LDCM0578  Fragment27 Ramos C42(0.64); C59(1.17); C243(0.99); C206(1.24)  LDD2197  [24]
 LDCM0586  Fragment28 Ramos C42(1.08); C59(1.02); C243(0.90); C206(1.02)  LDD2198  [24]
 LDCM0588  Fragment30 Ramos C42(0.84); C59(0.93); C243(1.12); C206(1.33)  LDD2199  [24]
 LDCM0589  Fragment31 Ramos C42(0.62); C59(1.67); C243(1.21); C206(1.45)  LDD2200  [24]
 LDCM0590  Fragment32 Ramos C42(0.75); C59(0.62); C243(0.59); C206(0.73)  LDD2201  [24]
 LDCM0468  Fragment33 HEK-293T C42(1.01); C100(0.77); C243(0.94); C206(1.19)  LDD1671  [23]
 LDCM0596  Fragment38 Ramos C42(0.58); C59(0.73); C243(0.86); C206(0.91)  LDD2203  [24]
 LDCM0566  Fragment4 Ramos C42(0.69); C59(0.77); C243(0.72); C206(0.66)  LDD2184  [24]
 LDCM0427  Fragment51 HEK-293T C42(0.90); C100(0.98); C243(0.92); C206(1.06)  LDD1631  [23]
 LDCM0610  Fragment52 Ramos C42(0.47); C59(0.86); C243(1.12); C206(1.27)  LDD2204  [24]
 LDCM0614  Fragment56 Ramos C42(0.87); C59(1.02); C243(1.44); C206(1.16)  LDD2205  [24]
 LDCM0569  Fragment7 Ramos C42(0.50); C59(0.41); C243(0.76); C206(0.55)  LDD2186  [24]
 LDCM0571  Fragment9 Ramos C42(0.54); C59(0.53); C243(0.58); C206(0.63)  LDD2188  [24]
 LDCM0107  IAA HeLa C59(0.00); C206(0.00)  LDD0221  [19]
 LDCM0022  KB02 HEK-293T C42(0.92); C100(1.01); C59(1.03); C249(1.01)  LDD1492  [23]
 LDCM0023  KB03 Jurkat C59(15.94)  LDD0315  [10]
 LDCM0024  KB05 HMCB C243(1.33); C59(1.76); C206(1.93); C45(1.99)  LDD3312  [4]
 LDCM0509  N-(4-bromo-3,5-dimethylphenyl)-2-nitroacetamide MDA-MB-231 C42(0.84)  LDD2102  [5]
 LDCM0528  N-(4-bromophenyl)-2-cyano-N-phenylacetamide MDA-MB-231 C42(0.84)  LDD2121  [5]
 LDCM0109  NEM HeLa H273(0.00); H278(0.00); H266(0.00)  LDD0223  [19]
 LDCM0496  Nucleophilic fragment 11a MDA-MB-231 C42(4.06)  LDD2089  [5]
 LDCM0499  Nucleophilic fragment 12b MDA-MB-231 C42(0.85)  LDD2092  [5]
 LDCM0500  Nucleophilic fragment 13a MDA-MB-231 C42(1.40)  LDD2093  [5]
 LDCM0504  Nucleophilic fragment 15a MDA-MB-231 C42(0.77)  LDD2097  [5]
 LDCM0506  Nucleophilic fragment 16a MDA-MB-231 C42(1.02)  LDD2099  [5]
 LDCM0507  Nucleophilic fragment 16b MDA-MB-231 C42(0.84); C59(1.03)  LDD2100  [5]
 LDCM0508  Nucleophilic fragment 17a MDA-MB-231 C42(1.03)  LDD2101  [5]
 LDCM0511  Nucleophilic fragment 18b MDA-MB-231 C59(0.61)  LDD2104  [5]
 LDCM0512  Nucleophilic fragment 19a MDA-MB-231 C42(0.98)  LDD2105  [5]
 LDCM0514  Nucleophilic fragment 20a MDA-MB-231 C42(1.24); C59(0.87)  LDD2107  [5]
 LDCM0515  Nucleophilic fragment 20b MDA-MB-231 C59(0.79)  LDD2108  [5]
 LDCM0516  Nucleophilic fragment 21a MDA-MB-231 C42(0.86); C59(0.61)  LDD2109  [5]
 LDCM0518  Nucleophilic fragment 22a MDA-MB-231 C42(1.04); C59(1.23)  LDD2111  [5]
 LDCM0521  Nucleophilic fragment 23b MDA-MB-231 C42(0.67); C59(0.34)  LDD2114  [5]
 LDCM0522  Nucleophilic fragment 24a MDA-MB-231 C42(1.03)  LDD2115  [5]
 LDCM0530  Nucleophilic fragment 28a MDA-MB-231 C42(0.86); C59(0.81)  LDD2123  [5]
 LDCM0531  Nucleophilic fragment 28b MDA-MB-231 C59(1.43)  LDD2124  [5]
 LDCM0532  Nucleophilic fragment 29a MDA-MB-231 C42(1.00); C59(0.76)  LDD2125  [5]
 LDCM0533  Nucleophilic fragment 29b MDA-MB-231 C59(0.79)  LDD2126  [5]
 LDCM0536  Nucleophilic fragment 31 MDA-MB-231 C42(1.00)  LDD2129  [5]
 LDCM0541  Nucleophilic fragment 36 MDA-MB-231 C42(0.62)  LDD2134  [5]
 LDCM0542  Nucleophilic fragment 37 MDA-MB-231 C42(0.84)  LDD2135  [5]
 LDCM0543  Nucleophilic fragment 38 MDA-MB-231 C42(1.16); C59(1.22)  LDD2136  [5]
 LDCM0544  Nucleophilic fragment 39 MDA-MB-231 C59(0.95)  LDD2137  [5]
 LDCM0211  Nucleophilic fragment 3b MDA-MB-231 C42(1.32)  LDD1700  [5]
 LDCM0546  Nucleophilic fragment 40 MDA-MB-231 C42(0.94); C59(0.86)  LDD2140  [5]
 LDCM0547  Nucleophilic fragment 41 MDA-MB-231 C42(0.67); C59(0.92)  LDD2141  [5]
 LDCM0551  Nucleophilic fragment 5b MDA-MB-231 C59(0.51)  LDD2145  [5]
 LDCM0552  Nucleophilic fragment 6a MDA-MB-231 C42(1.09)  LDD2146  [5]
 LDCM0555  Nucleophilic fragment 7b MDA-MB-231 C59(0.67)  LDD2149  [5]
 LDCM0627  NUDT7-COV-1 HEK-293T C42(0.60); C42(0.57)  LDD2206  [25]
 LDCM0628  OTUB2-COV-1 HEK-293T C42(1.20); C42(0.50)  LDD2207  [25]
 LDCM0131  RA190 MM1.R C42(1.04)  LDD0304  [26]

References

1 Quantitative and Site-Specific Chemoproteomic Profiling of Targets of Acrolein. Chem Res Toxicol. 2019 Mar 18;32(3):467-473. doi: 10.1021/acs.chemrestox.8b00343. Epub 2019 Jan 15.
2 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
3 An Azo Coupling-Based Chemoproteomic Approach to Systematically Profile the Tyrosine Reactivity in the Human Proteome. Anal Chem. 2021 Jul 27;93(29):10334-10342. doi: 10.1021/acs.analchem.1c01935. Epub 2021 Jul 12.
4 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
5 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
6 Chemoproteomic capture of RNA binding activity in living cells. Nat Commun. 2023 Oct 7;14(1):6282. doi: 10.1038/s41467-023-41844-z.
Mass spectrometry data entry: PXD044625
7 Global profiling of functional histidines in live cells using small-molecule photosensitizer and chemical probe relay labelling. Nat Chem. 2024 Jun 4. doi: 10.1038/s41557-024-01545-6. Online ahead of print.
Mass spectrometry data entry: PXD042377
8 Targeted Proteomic Approaches for Proteome-Wide Characterizations of the AMP-Binding Capacities of Kinases. J Proteome Res. 2022 Aug 5;21(8):2063-2070. doi: 10.1021/acs.jproteome.2c00225. Epub 2022 Jul 12.
9 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
10 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
11 SP3-Enabled Rapid and High Coverage Chemoproteomic Identification of Cell-State-Dependent Redox-Sensitive Cysteines. Mol Cell Proteomics. 2022 Apr;21(4):100218. doi: 10.1016/j.mcpro.2022.100218. Epub 2022 Feb 25.
Mass spectrometry data entry: PXD029500 , PXD031647
12 Comparison of Quantitative Mass Spectrometry Platforms for Monitoring Kinase ATP Probe Uptake in Lung Cancer. J Proteome Res. 2018 Jan 5;17(1):63-75. doi: 10.1021/acs.jproteome.7b00329. Epub 2017 Nov 22.
Mass spectrometry data entry: PXD006095 , PXD006096
13 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
14 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
15 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
16 A chemical probe unravels the reactive proteome of health-associated catechols. Chem Sci. 2023 Jul 22;14(32):8635-8643. doi: 10.1039/d3sc00888f. eCollection 2023 Aug 16.
Mass spectrometry data entry: PXD043348
17 Multiplexed CuAAC Suzuki-Miyaura Labeling for Tandem Activity-Based Chemoproteomic Profiling. Anal Chem. 2021 Feb 2;93(4):2610-2618. doi: 10.1021/acs.analchem.0c04726. Epub 2021 Jan 20.
Mass spectrometry data entry: PXD022279
18 DFT-Guided Discovery of Ethynyl-Triazolyl-Phosphinates as Modular Electrophiles for Chemoselective Cysteine Bioconjugation and Profiling. Angew Chem Int Ed Engl. 2022 Oct 10;61(41):e202205348. doi: 10.1002/anie.202205348. Epub 2022 Aug 22.
Mass spectrometry data entry: PXD033004
19 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
20 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
21 Design and synthesis of minimalist terminal alkyne-containing diazirine photo-crosslinkers and their incorporation into kinase inhibitors for cell- and tissue-based proteome profiling. Angew Chem Int Ed Engl. 2013 Aug 12;52(33):8551-6. doi: 10.1002/anie.201300683. Epub 2013 Jun 10.
22 Pharmacological Targeting of Vacuolar H(+)-ATPase via Subunit V1G Combats Multidrug-Resistant Cancer. Cell Chem Biol. 2020 Nov 19;27(11):1359-1370.e8. doi: 10.1016/j.chembiol.2020.06.011. Epub 2020 Jul 9.
23 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
24 Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry. Methods Enzymol. 2023;679:295-322. doi: 10.1016/bs.mie.2022.07.037. Epub 2022 Sep 7.
Mass spectrometry data entry: PXD027578
25 Rapid Covalent-Probe Discovery by Electrophile-Fragment Screening. J Am Chem Soc. 2019 Jun 5;141(22):8951-8968. doi: 10.1021/jacs.9b02822. Epub 2019 May 22.
26 Physical and Functional Analysis of the Putative Rpn13 Inhibitor RA190. Cell Chem Biol. 2020 Nov 19;27(11):1371-1382.e6. doi: 10.1016/j.chembiol.2020.08.007. Epub 2020 Aug 27.