Details of the Target

General Information of Target

Target ID LDTP12067
Target Name Protein ECT2 (ECT2)
Gene Name ECT2
Gene ID 1894
Synonyms
Protein ECT2; Epithelial cell-transforming sequence 2 oncogene
3D Structure
Download
2D Sequence (FASTA)
Download
3D Structure (PDB)
Download
Sequence
MNPFWSMSTSSVRKRSEGEEKTLTGDVKTSPPRTAPKKQLPSIPKNALPITKPTSPAPAA
QSTNGTHASYGPFYLEYSLLAEFTLVVKQKLPGVYVQPSYRSALMWFGVIFIRHGLYQDG
VFKFTVYIPDNYPDGDCPRLVFDIPVFHPLVDPTSGELDVKRAFAKWRRNHNHIWQVLMY
ARRVFYKIDTASPLNPEAAVLYEKDIQLFKSKVVDSVKVCTARLFDQPKIEDPYAISFSP
WNPSVHDEAREKMLTQKKPEEQHNKSVHVAGLSWVKPGSVQPFSKEEKTVAT
Target Bioclass
Other
Subcellular location
Nucleus
Function
Guanine nucleotide exchange factor (GEF) that catalyzes the exchange of GDP for GTP. Promotes guanine nucleotide exchange on the Rho family members of small GTPases, like RHOA, RHOC, RAC1 and CDC42. Required for signal transduction pathways involved in the regulation of cytokinesis. Component of the centralspindlin complex that serves as a microtubule-dependent and Rho-mediated signaling required for the myosin contractile ring formation during the cell cycle cytokinesis. Regulates the translocation of RHOA from the central spindle to the equatorial region. Plays a role in the control of mitotic spindle assembly; regulates the activation of CDC42 in metaphase for the process of spindle fibers attachment to kinetochores before chromosome congression. Involved in the regulation of epithelial cell polarity; participates in the formation of epithelial tight junctions in a polarity complex PARD3-PARD6-protein kinase PRKCQ-dependent manner. Plays a role in the regulation of neurite outgrowth. Inhibits phenobarbital (PB)-induced NR1I3 nuclear translocation. Stimulates the activity of RAC1 through its association with the oncogenic PARD6A-PRKCI complex in cancer cells, thereby acting to coordinately drive tumor cell proliferation and invasion. Also stimulates genotoxic stress-induced RHOB activity in breast cancer cells leading to their cell death.
Uniprot ID
Q9H8V3
Ensemble ID
ENST00000232458.9
HGNC ID
HGNC:3155

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
ETK1 SNV: p.G106S DBIA    Probe Info 
HCT15 SNV: p.M351L .
HEC1 SNV: p.L416P DBIA    Probe Info 
MFE319 SNV: p.Q134R DBIA    Probe Info 
SSP25 SNV: p.G106S DBIA    Probe Info 

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 18 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
m-APA
 Probe Info  		11.71  LDD0402  [1]
BTD
 Probe Info  		C564(1.41); C584(1.02); C221(0.84)  LDD1700  [2]
AHL-Pu-1
 Probe Info  		C274(4.49)  LDD0170  [3]
DBIA
 Probe Info  		C221(4.04)  LDD0209  [4]
ATP probe
 Probe Info  		N.A.  LDD0199  [5]
4-Iodoacetamidophenylacetylene
 Probe Info  		C796(0.00); C157(0.00); C308(0.00); C221(0.00)  LDD0038  [6]
IA-alkyne
 Probe Info  		C308(0.00); C661(0.00); C221(0.00); C796(0.00)  LDD0036  [6]
IPIAA_L
 Probe Info  		N.A.  LDD0031  [7]
Lodoacetamide azide
 Probe Info  		C308(0.00); C661(0.00); C686(0.00); C157(0.00)  LDD0037  [6]
Compound 10
 Probe Info  		N.A.  LDD2216  [8]
Compound 11
 Probe Info  		N.A.  LDD2213  [8]
IPM
 Probe Info  		N.A.  LDD0005  [9]
TFBX
 Probe Info  		C686(0.00); C796(0.00); C584(0.00)  LDD0148  [10]
Phosphinate-6
 Probe Info  		C86(0.00); C221(0.00)  LDD0018  [11]
Acrolein
 Probe Info  		C221(0.00); C686(0.00); C584(0.00)  LDD0217  [12]
Methacrolein
 Probe Info  		N.A.  LDD0218  [12]
AOyne
 Probe Info  		14.70  LDD0443  [13]
NAIA_5
 Probe Info  		C308(0.00); C584(0.00)  LDD2223  [14]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0548  1-(4-(Benzo[d][1,3]dioxol-5-ylmethyl)piperazin-1-yl)-2-nitroethan-1-one MDA-MB-231 C221(0.68); C584(0.79)  LDD2142  [2]
 LDCM0519  1-(6-methoxy-3,4-dihydroquinolin-1(2H)-yl)-2-nitroethan-1-one MDA-MB-231 C221(0.92); C584(0.93)  LDD2112  [2]
 LDCM0524  2-Cyano-N-(2-morpholin-4-yl-ethyl)-acetamide MDA-MB-231 C221(1.05); C584(0.74); C564(0.85)  LDD2117  [2]
 LDCM0558  2-Cyano-N-phenylacetamide MDA-MB-231 C221(1.26)  LDD2152  [2]
 LDCM0510  3-(4-(Hydroxydiphenylmethyl)piperidin-1-yl)-3-oxopropanenitrile MDA-MB-231 C221(0.99); C584(0.86)  LDD2103  [2]
 LDCM0025  4SU-RNA DM93 C274(4.49)  LDD0170  [3]
 LDCM0214  AC1 HEK-293T C584(0.96); C796(0.94); C221(0.96); C157(0.94)  LDD1507  [15]
 LDCM0215  AC10 HEK-293T C584(1.09); C796(1.01); C221(0.92); C157(1.02)  LDD1508  [15]
 LDCM0226  AC11 HEK-293T C584(0.99); C796(0.92); C221(0.92); C157(0.94)  LDD1509  [15]
 LDCM0237  AC12 HEK-293T C584(1.16); C796(0.98); C221(0.96); C157(1.00)  LDD1510  [15]
 LDCM0259  AC14 HEK-293T C584(0.94); C796(0.95); C221(0.94); C157(0.95)  LDD1512  [15]
 LDCM0270  AC15 HEK-293T C584(1.05); C796(1.03); C221(0.93); C157(0.95)  LDD1513  [15]
 LDCM0276  AC17 HEK-293T C584(0.99); C796(0.89); C221(1.04); C157(0.79)  LDD1515  [15]
 LDCM0277  AC18 HEK-293T C584(0.97); C796(0.95); C221(1.04); C157(1.03)  LDD1516  [15]
 LDCM0278  AC19 HEK-293T C584(1.10); C796(0.96); C221(0.87); C157(1.06)  LDD1517  [15]
 LDCM0279  AC2 HEK-293T C584(1.06); C796(1.12); C221(0.92); C157(1.06)  LDD1518  [15]
 LDCM0280  AC20 HEK-293T C584(1.12); C796(1.06); C221(1.01); C157(1.01)  LDD1519  [15]
 LDCM0281  AC21 HEK-293T C584(0.87); C796(0.95); C221(1.01); C157(1.21)  LDD1520  [15]
 LDCM0282  AC22 HEK-293T C584(0.93); C796(0.98); C221(1.03); C157(1.08)  LDD1521  [15]
 LDCM0283  AC23 HEK-293T C584(1.10); C796(1.07); C221(0.98); C157(0.98)  LDD1522  [15]
 LDCM0284  AC24 HEK-293T C584(1.05); C796(1.02); C221(0.96); C157(1.10)  LDD1523  [15]
 LDCM0285  AC25 HEK-293T C584(0.96); C796(0.96); C221(1.00); C157(0.84)  LDD1524  [15]
 LDCM0286  AC26 HEK-293T C584(1.10); C796(1.02); C221(0.98); C157(0.99)  LDD1525  [15]
 LDCM0287  AC27 HEK-293T C584(1.00); C796(0.92); C221(0.90); C157(0.91)  LDD1526  [15]
 LDCM0288  AC28 HEK-293T C584(1.10); C796(0.99); C221(0.98); C157(1.04)  LDD1527  [15]
 LDCM0289  AC29 HEK-293T C584(0.92); C796(0.88); C221(1.03); C157(0.95)  LDD1528  [15]
 LDCM0290  AC3 HEK-293T C584(1.00); C796(0.93); C221(1.01); C157(1.06)  LDD1529  [15]
 LDCM0291  AC30 HEK-293T C584(1.02); C796(0.96); C221(0.97); C157(1.01)  LDD1530  [15]
 LDCM0292  AC31 HEK-293T C584(1.05); C796(0.91); C221(0.97); C157(0.97)  LDD1531  [15]
 LDCM0293  AC32 HEK-293T C584(1.06); C796(0.99); C221(0.94); C157(1.02)  LDD1532  [15]
 LDCM0294  AC33 HEK-293T C584(0.99); C796(0.96); C221(0.97); C157(1.01)  LDD1533  [15]
 LDCM0295  AC34 HEK-293T C584(0.98); C796(1.02); C221(1.05); C157(1.08)  LDD1534  [15]
 LDCM0296  AC35 HEK-293T C584(1.06); C796(0.98); C221(0.96); C157(1.10)  LDD1535  [15]
 LDCM0297  AC36 HEK-293T C584(1.21); C796(0.94); C221(0.97); C157(1.08)  LDD1536  [15]
 LDCM0298  AC37 HEK-293T C584(0.76); C796(0.91); C221(0.93); C157(1.14)  LDD1537  [15]
 LDCM0299  AC38 HEK-293T C584(1.00); C796(1.01); C221(0.94); C157(1.08)  LDD1538  [15]
 LDCM0300  AC39 HEK-293T C584(1.19); C796(1.15); C221(0.95); C157(0.91)  LDD1539  [15]
 LDCM0301  AC4 HEK-293T C584(1.05); C796(0.93); C221(1.05); C157(1.01)  LDD1540  [15]
 LDCM0302  AC40 HEK-293T C584(1.12); C796(0.96); C221(1.06); C157(1.05)  LDD1541  [15]
 LDCM0303  AC41 HEK-293T C584(1.00); C796(0.93); C221(0.97); C157(0.86)  LDD1542  [15]
 LDCM0304  AC42 HEK-293T C584(0.94); C796(1.04); C221(0.92); C157(1.09)  LDD1543  [15]
 LDCM0305  AC43 HEK-293T C584(1.08); C796(0.97); C221(0.90); C157(1.04)  LDD1544  [15]
 LDCM0306  AC44 HEK-293T C584(1.08); C796(0.97); C221(0.94); C157(0.97)  LDD1545  [15]
 LDCM0307  AC45 HEK-293T C584(1.13); C796(0.89); C221(1.05); C157(1.03)  LDD1546  [15]
 LDCM0308  AC46 HEK-293T C584(1.04); C796(0.89); C221(0.99); C157(1.15)  LDD1547  [15]
 LDCM0309  AC47 HEK-293T C584(1.13); C796(1.08); C221(0.96); C157(1.04)  LDD1548  [15]
 LDCM0310  AC48 HEK-293T C584(1.10); C796(1.00); C221(1.04); C157(1.02)  LDD1549  [15]
 LDCM0311  AC49 HEK-293T C584(1.04); C796(0.99); C221(0.96); C157(0.94)  LDD1550  [15]
 LDCM0312  AC5 HEK-293T C584(0.90); C796(0.92); C221(0.99); C157(1.03)  LDD1551  [15]
 LDCM0313  AC50 HEK-293T C584(1.03); C796(1.08); C221(0.95); C157(1.05)  LDD1552  [15]
 LDCM0314  AC51 HEK-293T C584(1.08); C796(0.95); C221(0.92); C157(1.07)  LDD1553  [15]
 LDCM0315  AC52 HEK-293T C584(1.13); C796(0.92); C221(0.98); C157(0.97)  LDD1554  [15]
 LDCM0316  AC53 HEK-293T C584(0.87); C796(0.91); C221(0.98); C157(1.00)  LDD1555  [15]
 LDCM0317  AC54 HEK-293T C584(1.02); C796(0.98); C221(1.01); C157(0.98)  LDD1556  [15]
 LDCM0318  AC55 HEK-293T C584(1.13); C796(0.97); C221(1.00); C157(0.94)  LDD1557  [15]
 LDCM0319  AC56 HEK-293T C584(1.07); C796(0.92); C221(1.09); C157(1.07)  LDD1558  [15]
 LDCM0320  AC57 HEK-293T C584(0.97); C796(0.95); C221(0.98); C157(0.95)  LDD1559  [15]
 LDCM0321  AC58 HEK-293T C584(1.05); C796(1.04); C221(0.96); C157(0.97)  LDD1560  [15]
 LDCM0322  AC59 HEK-293T C584(1.07); C796(0.99); C221(0.90); C157(0.94)  LDD1561  [15]
 LDCM0323  AC6 HEK-293T C584(0.88); C796(0.94); C221(1.06); C157(1.09)  LDD1562  [15]
 LDCM0324  AC60 HEK-293T C584(1.16); C796(0.93); C221(1.02); C157(1.19)  LDD1563  [15]
 LDCM0325  AC61 HEK-293T C584(0.94); C796(0.96); C221(1.05); C157(1.09)  LDD1564  [15]
 LDCM0326  AC62 HEK-293T C584(0.99); C796(0.91); C221(0.96); C157(1.07)  LDD1565  [15]
 LDCM0327  AC63 HEK-293T C584(0.99); C796(1.02); C221(0.92); C157(1.02)  LDD1566  [15]
 LDCM0328  AC64 HEK-293T C584(1.12); C796(0.91); C221(0.97); C157(1.05)  LDD1567  [15]
 LDCM0334  AC7 HEK-293T C584(1.06); C796(1.03); C221(1.06); C157(0.94)  LDD1568  [15]
 LDCM0345  AC8 HEK-293T C584(1.07); C796(0.99); C221(1.11); C157(1.07)  LDD1569  [15]
 LDCM0545  Acetamide MDA-MB-231 C221(0.43); C584(0.29)  LDD2138  [2]
 LDCM0248  AKOS034007472 HEK-293T C584(1.09); C796(0.96); C221(1.01); C157(1.06)  LDD1511  [15]
 LDCM0356  AKOS034007680 HEK-293T C584(0.98); C796(0.89); C221(0.93); C157(0.95)  LDD1570  [15]
 LDCM0275  AKOS034007705 HEK-293T C584(1.08); C796(0.91); C221(1.05); C157(1.00)  LDD1514  [15]
 LDCM0156  Aniline NCI-H1299 11.40  LDD0403  [1]
 LDCM0498  BS-3668 MDA-MB-231 C584(0.50)  LDD2091  [2]
 LDCM0630  CCW28-3 231MFP C221(1.79)  LDD2214  [16]
 LDCM0632  CL-Sc Hep-G2 C686(0.96); C221(0.89); C221(0.44)  LDD2227  [14]
 LDCM0367  CL1 HEK-293T C584(0.94); C796(1.10); C221(0.89); C157(0.95)  LDD1571  [15]
 LDCM0368  CL10 HEK-293T C584(0.95); C796(0.92); C221(1.19); C157(1.00)  LDD1572  [15]
 LDCM0369  CL100 HEK-293T C584(1.09); C796(0.95); C221(0.94); C157(1.04)  LDD1573  [15]
 LDCM0370  CL101 HEK-293T C584(0.91); C796(0.93); C221(0.97); C157(1.01)  LDD1574  [15]
 LDCM0371  CL102 HEK-293T C584(1.04); C796(0.86); C221(1.09); C157(1.04)  LDD1575  [15]
 LDCM0372  CL103 HEK-293T C796(1.01); C221(0.86); C157(1.03); C661(1.15)  LDD1576  [15]
 LDCM0373  CL104 HEK-293T C584(1.12); C796(0.99); C221(0.97); C157(0.97)  LDD1577  [15]
 LDCM0374  CL105 HEK-293T C584(0.92); C796(0.92); C221(0.99); C157(1.04)  LDD1578  [15]
 LDCM0375  CL106 HEK-293T C584(0.91); C796(0.90); C221(0.97); C157(1.03)  LDD1579  [15]
 LDCM0376  CL107 HEK-293T C796(0.98); C221(0.96); C157(1.04); C661(1.02)  LDD1580  [15]
 LDCM0377  CL108 HEK-293T C584(1.12); C796(0.97); C221(1.05); C157(1.00)  LDD1581  [15]
 LDCM0378  CL109 HEK-293T C584(0.96); C796(0.92); C221(0.84); C157(0.97)  LDD1582  [15]
 LDCM0379  CL11 HEK-293T C584(1.05); C796(0.77); C221(1.14); C157(0.94)  LDD1583  [15]
 LDCM0380  CL110 HEK-293T C584(0.97); C796(0.90); C221(1.04); C157(0.95)  LDD1584  [15]
 LDCM0381  CL111 HEK-293T C796(1.02); C221(0.87); C157(0.99); C661(0.89)  LDD1585  [15]
 LDCM0382  CL112 HEK-293T C584(1.03); C796(0.93); C221(1.20); C157(1.01)  LDD1586  [15]
 LDCM0383  CL113 HEK-293T C584(0.97); C796(0.98); C221(0.95); C157(0.98)  LDD1587  [15]
 LDCM0384  CL114 HEK-293T C584(1.10); C796(0.94); C221(1.10); C157(1.03)  LDD1588  [15]
 LDCM0385  CL115 HEK-293T C796(1.02); C221(0.98); C157(1.06); C661(1.08)  LDD1589  [15]
 LDCM0386  CL116 HEK-293T C584(1.14); C796(0.97); C221(1.00); C157(1.22)  LDD1590  [15]
 LDCM0387  CL117 HEK-293T C584(0.97); C796(0.90); C221(0.93); C157(1.08)  LDD1591  [15]
 LDCM0388  CL118 HEK-293T C584(1.03); C796(0.93); C221(0.98); C157(1.03)  LDD1592  [15]
 LDCM0389  CL119 HEK-293T C796(1.00); C221(0.83); C157(1.03); C661(1.06)  LDD1593  [15]
 LDCM0390  CL12 HEK-293T C584(1.06); C796(0.91); C221(1.14); C157(1.29)  LDD1594  [15]
 LDCM0391  CL120 HEK-293T C584(1.17); C796(0.96); C221(0.96); C157(0.94)  LDD1595  [15]
 LDCM0392  CL121 HEK-293T C584(0.92); C796(0.90); C221(0.97); C157(1.11)  LDD1596  [15]
 LDCM0393  CL122 HEK-293T C584(1.06); C796(0.91); C221(1.00); C157(0.96)  LDD1597  [15]
 LDCM0394  CL123 HEK-293T C796(0.95); C221(0.84); C157(0.96); C661(1.24)  LDD1598  [15]
 LDCM0395  CL124 HEK-293T C584(1.12); C796(0.87); C221(0.98); C157(1.08)  LDD1599  [15]
 LDCM0396  CL125 HEK-293T C584(0.94); C796(0.87); C221(0.94); C157(1.13)  LDD1600  [15]
 LDCM0397  CL126 HEK-293T C584(1.05); C796(0.98); C221(1.03); C157(1.10)  LDD1601  [15]
 LDCM0398  CL127 HEK-293T C796(0.97); C221(0.92); C157(1.12); C661(0.95)  LDD1602  [15]
 LDCM0399  CL128 HEK-293T C584(1.09); C796(0.96); C221(1.02); C157(1.11)  LDD1603  [15]
 LDCM0400  CL13 HEK-293T C584(0.90); C796(0.93); C221(1.00); C157(1.01)  LDD1604  [15]
 LDCM0401  CL14 HEK-293T C584(1.10); C796(0.92); C221(1.07); C157(1.03)  LDD1605  [15]
 LDCM0402  CL15 HEK-293T C796(0.91); C221(0.78); C157(1.11); C661(0.78)  LDD1606  [15]
 LDCM0403  CL16 HEK-293T C584(1.22); C796(1.02); C221(1.01); C157(1.09)  LDD1607  [15]
 LDCM0404  CL17 HEK-293T C584(0.92); C796(0.93); C221(0.99); C157(0.81)  LDD1608  [15]
 LDCM0405  CL18 HEK-293T C584(0.94); C796(0.99); C221(0.93); C157(1.06)  LDD1609  [15]
 LDCM0406  CL19 HEK-293T C584(1.04); C796(0.93); C221(0.98); C157(0.89)  LDD1610  [15]
 LDCM0407  CL2 HEK-293T C584(1.09); C796(0.97); C221(1.08); C157(1.01)  LDD1611  [15]
 LDCM0408  CL20 HEK-293T C584(1.15); C796(0.93); C221(1.05); C157(1.02)  LDD1612  [15]
 LDCM0409  CL21 HEK-293T C584(1.22); C796(0.77); C221(1.19); C157(1.00)  LDD1613  [15]
 LDCM0410  CL22 HEK-293T C584(0.96); C796(0.98); C221(1.21); C157(1.02)  LDD1614  [15]
 LDCM0411  CL23 HEK-293T C584(1.07); C796(0.91); C221(1.08); C157(0.95)  LDD1615  [15]
 LDCM0412  CL24 HEK-293T C584(1.01); C796(0.95); C221(1.24); C157(1.31)  LDD1616  [15]
 LDCM0413  CL25 HEK-293T C584(0.90); C796(0.83); C221(0.83); C157(0.98)  LDD1617  [15]
 LDCM0414  CL26 HEK-293T C584(1.00); C796(0.94); C221(1.01); C157(1.10)  LDD1618  [15]
 LDCM0415  CL27 HEK-293T C796(1.01); C221(0.87); C157(1.10); C661(0.99)  LDD1619  [15]
 LDCM0416  CL28 HEK-293T C584(0.99); C796(0.99); C221(0.97); C157(1.09)  LDD1620  [15]
 LDCM0417  CL29 HEK-293T C584(0.94); C796(0.98); C221(0.99); C157(1.14)  LDD1621  [15]
 LDCM0418  CL3 HEK-293T C796(0.96); C221(0.99); C157(1.06); C661(0.98)  LDD1622  [15]
 LDCM0419  CL30 HEK-293T C584(1.07); C796(1.05); C221(1.02); C157(1.03)  LDD1623  [15]
 LDCM0420  CL31 HEK-293T C584(1.00); C796(0.90); C221(1.02); C157(1.11)  LDD1624  [15]
 LDCM0421  CL32 HEK-293T C584(1.12); C796(0.99); C221(1.01); C157(0.94)  LDD1625  [15]
 LDCM0422  CL33 HEK-293T C584(0.84); C796(0.90); C221(1.01); C157(0.94)  LDD1626  [15]
 LDCM0423  CL34 HEK-293T C584(0.84); C796(0.95); C221(1.05); C157(0.93)  LDD1627  [15]
 LDCM0424  CL35 HEK-293T C584(1.07); C796(1.16); C221(1.11); C157(1.07)  LDD1628  [15]
 LDCM0425  CL36 HEK-293T C584(0.98); C796(0.97); C221(1.04); C157(1.19)  LDD1629  [15]
 LDCM0426  CL37 HEK-293T C584(1.01); C796(0.82); C221(0.92); C157(1.06)  LDD1630  [15]
 LDCM0428  CL39 HEK-293T C796(0.96); C221(1.16); C157(0.95); C661(0.88)  LDD1632  [15]
 LDCM0429  CL4 HEK-293T C584(1.14); C796(1.01); C221(1.00); C157(1.02)  LDD1633  [15]
 LDCM0430  CL40 HEK-293T C584(1.04); C796(1.04); C221(0.95); C157(0.88)  LDD1634  [15]
 LDCM0431  CL41 HEK-293T C584(0.95); C796(0.95); C221(1.07); C157(0.90)  LDD1635  [15]
 LDCM0432  CL42 HEK-293T C584(1.10); C796(1.01); C221(0.96); C157(1.10)  LDD1636  [15]
 LDCM0433  CL43 HEK-293T C584(1.06); C796(0.95); C221(0.91); C157(0.86)  LDD1637  [15]
 LDCM0434  CL44 HEK-293T C584(0.88); C796(0.94); C221(1.01); C157(0.99)  LDD1638  [15]
 LDCM0435  CL45 HEK-293T C584(0.78); C796(0.86); C221(1.16); C157(0.91)  LDD1639  [15]
 LDCM0436  CL46 HEK-293T C584(0.83); C796(0.87); C221(1.07); C157(0.94)  LDD1640  [15]
 LDCM0437  CL47 HEK-293T C584(0.88); C796(0.93); C221(1.04); C157(0.89)  LDD1641  [15]
 LDCM0438  CL48 HEK-293T C584(0.98); C796(0.97); C221(1.07); C157(1.15)  LDD1642  [15]
 LDCM0439  CL49 HEK-293T C584(0.93); C796(1.18); C221(0.84); C157(1.18)  LDD1643  [15]
 LDCM0440  CL5 HEK-293T C584(1.01); C796(0.99); C221(0.98); C157(0.70)  LDD1644  [15]
 LDCM0441  CL50 HEK-293T C584(1.01); C796(0.92); C221(1.04); C157(0.95)  LDD1645  [15]
 LDCM0443  CL52 HEK-293T C584(1.10); C796(1.01); C221(0.94); C157(1.01)  LDD1646  [15]
 LDCM0444  CL53 HEK-293T C584(0.87); C796(0.83); C221(1.00); C157(0.78)  LDD1647  [15]
 LDCM0445  CL54 HEK-293T C584(0.93); C796(0.99); C221(0.82); C157(0.94)  LDD1648  [15]
 LDCM0446  CL55 HEK-293T C584(0.97); C796(0.95); C221(0.93); C157(1.03)  LDD1649  [15]
 LDCM0447  CL56 HEK-293T C584(1.09); C796(1.00); C221(1.17); C157(0.99)  LDD1650  [15]
 LDCM0448  CL57 HEK-293T C584(0.81); C796(0.85); C221(1.09); C157(1.05)  LDD1651  [15]
 LDCM0449  CL58 HEK-293T C584(0.88); C796(0.89); C221(1.07); C157(1.04)  LDD1652  [15]
 LDCM0450  CL59 HEK-293T C584(0.99); C796(0.96); C221(1.04); C157(0.88)  LDD1653  [15]
 LDCM0451  CL6 HEK-293T C584(0.91); C796(0.93); C221(0.93); C157(1.01)  LDD1654  [15]
 LDCM0452  CL60 HEK-293T C584(1.03); C796(0.91); C221(1.11); C157(1.23)  LDD1655  [15]
 LDCM0453  CL61 HEK-293T C584(0.99); C796(0.96); C221(0.86); C157(1.11)  LDD1656  [15]
 LDCM0454  CL62 HEK-293T C584(1.12); C796(0.94); C221(1.05); C157(1.11)  LDD1657  [15]
 LDCM0455  CL63 HEK-293T C796(0.98); C221(0.91); C157(1.15); C661(1.07)  LDD1658  [15]
 LDCM0456  CL64 HEK-293T C584(1.07); C796(0.93); C221(0.90); C157(0.98)  LDD1659  [15]
 LDCM0457  CL65 HEK-293T C584(1.00); C796(0.95); C221(0.94); C157(0.86)  LDD1660  [15]
 LDCM0458  CL66 HEK-293T C584(1.01); C796(0.98); C221(0.94); C157(1.02)  LDD1661  [15]
 LDCM0459  CL67 HEK-293T C584(1.06); C796(0.89); C221(0.98); C157(0.94)  LDD1662  [15]
 LDCM0460  CL68 HEK-293T C584(1.12); C796(0.98); C221(0.98); C157(1.05)  LDD1663  [15]
 LDCM0461  CL69 HEK-293T C584(1.00); C796(0.90); C221(1.02); C157(0.99)  LDD1664  [15]
 LDCM0462  CL7 HEK-293T C584(1.12); C796(0.97); C221(0.95); C157(1.10)  LDD1665  [15]
 LDCM0463  CL70 HEK-293T C584(0.98); C796(0.97); C221(1.08); C157(0.97)  LDD1666  [15]
 LDCM0464  CL71 HEK-293T C584(1.02); C796(1.05); C221(1.04); C157(0.98)  LDD1667  [15]
 LDCM0465  CL72 HEK-293T C584(1.01); C796(0.94); C221(1.10); C157(1.17)  LDD1668  [15]
 LDCM0466  CL73 HEK-293T C584(1.02); C796(0.78); C221(0.85); C157(1.14)  LDD1669  [15]
 LDCM0467  CL74 HEK-293T C584(1.11); C796(0.96); C221(1.05); C157(1.00)  LDD1670  [15]
 LDCM0469  CL76 HEK-293T C584(1.06); C796(1.02); C221(0.93); C157(1.02)  LDD1672  [15]
 LDCM0470  CL77 HEK-293T C584(0.96); C796(0.92); C221(1.09); C157(0.95)  LDD1673  [15]
 LDCM0471  CL78 HEK-293T C584(1.18); C796(1.04); C221(1.00); C157(1.05)  LDD1674  [15]
 LDCM0472  CL79 HEK-293T C584(1.02); C796(0.93); C221(0.94); C157(0.95)  LDD1675  [15]
 LDCM0473  CL8 HEK-293T C584(1.03); C796(0.90); C221(0.98); C157(1.01)  LDD1676  [15]
 LDCM0474  CL80 HEK-293T C584(0.92); C796(0.96); C221(1.00); C157(1.05)  LDD1677  [15]
 LDCM0475  CL81 HEK-293T C584(0.89); C796(0.86); C221(1.07); C157(0.97)  LDD1678  [15]
 LDCM0476  CL82 HEK-293T C584(0.97); C796(0.92); C221(1.04); C157(0.99)  LDD1679  [15]
 LDCM0477  CL83 HEK-293T C584(1.02); C796(1.06); C221(1.03); C157(1.05)  LDD1680  [15]
 LDCM0478  CL84 HEK-293T C584(0.98); C796(0.89); C221(1.10); C157(1.13)  LDD1681  [15]
 LDCM0479  CL85 HEK-293T C584(0.96); C796(1.04); C221(0.83); C157(1.00)  LDD1682  [15]
 LDCM0480  CL86 HEK-293T C584(1.07); C796(0.97); C221(1.02); C157(1.10)  LDD1683  [15]
 LDCM0481  CL87 HEK-293T C796(0.98); C221(0.92); C157(1.14); C661(0.84)  LDD1684  [15]
 LDCM0482  CL88 HEK-293T C584(1.20); C796(1.03); C221(0.95); C157(1.01)  LDD1685  [15]
 LDCM0483  CL89 HEK-293T C584(0.98); C796(0.96); C221(0.97); C157(0.93)  LDD1686  [15]
 LDCM0484  CL9 HEK-293T C584(0.95); C796(0.98); C221(1.13); C157(1.01)  LDD1687  [15]
 LDCM0485  CL90 HEK-293T C584(1.15); C796(0.95); C221(0.92); C157(0.89)  LDD1688  [15]
 LDCM0486  CL91 HEK-293T C584(1.14); C796(0.98); C221(0.94); C157(1.17)  LDD1689  [15]
 LDCM0487  CL92 HEK-293T C584(1.02); C796(0.92); C221(0.96); C157(1.06)  LDD1690  [15]
 LDCM0488  CL93 HEK-293T C584(0.95); C796(0.82); C221(1.03); C157(1.04)  LDD1691  [15]
 LDCM0489  CL94 HEK-293T C584(0.91); C796(0.89); C221(1.12); C157(1.02)  LDD1692  [15]
 LDCM0490  CL95 HEK-293T C584(1.15); C796(0.89); C221(1.22); C157(0.89)  LDD1693  [15]
 LDCM0491  CL96 HEK-293T C584(1.11); C796(0.88); C221(1.08); C157(1.02)  LDD1694  [15]
 LDCM0492  CL97 HEK-293T C584(0.88); C796(1.04); C221(0.88); C157(0.98)  LDD1695  [15]
 LDCM0493  CL98 HEK-293T C584(0.96); C796(0.89); C221(1.02); C157(1.06)  LDD1696  [15]
 LDCM0494  CL99 HEK-293T C796(0.99); C221(1.03); C157(1.03); C661(0.97)  LDD1697  [15]
 LDCM0495  E2913 HEK-293T C796(1.01); C221(1.01); C157(1.06); C661(1.26)  LDD1698  [15]
 LDCM0213  Electrophilic fragment 2 MDA-MB-231 C686(4.28); C796(3.77); C661(3.71); C584(3.35)  LDD1702  [2]
 LDCM0625  F8 Ramos C584(0.99); C796(1.93); C221(1.59)  LDD2187  [17]
 LDCM0572  Fragment10 Ramos C584(0.62); C221(2.25)  LDD2189  [17]
 LDCM0573  Fragment11 Ramos C584(0.83); C796(13.41); C221(16.65)  LDD2190  [17]
 LDCM0574  Fragment12 Ramos C584(0.85); C221(0.74)  LDD2191  [17]
 LDCM0575  Fragment13 Ramos C584(0.86); C221(0.93)  LDD2192  [17]
 LDCM0576  Fragment14 Ramos C584(0.67); C221(0.89)  LDD2193  [17]
 LDCM0579  Fragment20 Ramos C584(0.80); C221(0.45)  LDD2194  [17]
 LDCM0580  Fragment21 Ramos C584(0.76); C221(0.91)  LDD2195  [17]
 LDCM0582  Fragment23 Ramos C584(0.44); C221(0.71)  LDD2196  [17]
 LDCM0578  Fragment27 Ramos C584(0.95); C221(0.87)  LDD2197  [17]
 LDCM0586  Fragment28 Ramos C584(0.88); C796(0.54); C221(0.80)  LDD2198  [17]
 LDCM0588  Fragment30 Ramos C584(1.11); C221(1.04)  LDD2199  [17]
 LDCM0589  Fragment31 Ramos C584(0.69); C221(0.63)  LDD2200  [17]
 LDCM0590  Fragment32 Ramos C584(1.22); C221(5.14)  LDD2201  [17]
 LDCM0468  Fragment33 HEK-293T C796(1.00); C221(0.93); C157(1.22); C661(1.08)  LDD1671  [15]
 LDCM0596  Fragment38 Ramos C584(0.90); C221(0.85)  LDD2203  [17]
 LDCM0566  Fragment4 Ramos C584(0.77); C796(1.09); C221(1.44)  LDD2184  [17]
 LDCM0427  Fragment51 HEK-293T C584(1.06); C796(0.99); C221(0.97); C157(1.08)  LDD1631  [15]
 LDCM0610  Fragment52 Ramos C584(1.33); C221(0.99)  LDD2204  [17]
 LDCM0614  Fragment56 Ramos C584(1.28); C221(0.75)  LDD2205  [17]
 LDCM0569  Fragment7 Ramos C584(0.64); C796(0.64); C221(1.42)  LDD2186  [17]
 LDCM0571  Fragment9 Ramos C584(0.80); C221(1.05)  LDD2188  [17]
 LDCM0022  KB02 HEK-293T C584(0.95); C796(0.96); C221(0.99); C308(1.02)  LDD1492  [15]
 LDCM0023  KB03 Jurkat C221(4.04)  LDD0209  [4]
 LDCM0024  KB05 COLO792 C584(3.05); C157(2.41)  LDD3310  [18]
 LDCM0509  N-(4-bromo-3,5-dimethylphenyl)-2-nitroacetamide MDA-MB-231 C584(0.99)  LDD2102  [2]
 LDCM0528  N-(4-bromophenyl)-2-cyano-N-phenylacetamide MDA-MB-231 C221(0.81); C584(0.61)  LDD2121  [2]
 LDCM0496  Nucleophilic fragment 11a MDA-MB-231 C221(0.50)  LDD2089  [2]
 LDCM0497  Nucleophilic fragment 11b MDA-MB-231 C221(1.11); C584(1.34)  LDD2090  [2]
 LDCM0499  Nucleophilic fragment 12b MDA-MB-231 C584(1.01)  LDD2092  [2]
 LDCM0500  Nucleophilic fragment 13a MDA-MB-231 C584(0.85); C564(0.52); C796(1.05)  LDD2093  [2]
 LDCM0501  Nucleophilic fragment 13b MDA-MB-231 C221(1.16); C584(1.14)  LDD2094  [2]
 LDCM0503  Nucleophilic fragment 14b MDA-MB-231 C221(0.49); C584(0.49); C796(1.19)  LDD2096  [2]
 LDCM0505  Nucleophilic fragment 15b MDA-MB-231 C221(0.94); C584(0.66); C564(0.97)  LDD2098  [2]
 LDCM0506  Nucleophilic fragment 16a MDA-MB-231 C221(0.96); C584(0.60); C796(1.04)  LDD2099  [2]
 LDCM0511  Nucleophilic fragment 18b MDA-MB-231 C221(1.08); C584(1.02)  LDD2104  [2]
 LDCM0512  Nucleophilic fragment 19a MDA-MB-231 C221(1.38); C584(1.15)  LDD2105  [2]
 LDCM0513  Nucleophilic fragment 19b MDA-MB-231 C221(0.69); C584(0.97)  LDD2106  [2]
 LDCM0514  Nucleophilic fragment 20a MDA-MB-231 C221(1.00); C584(0.85); C564(0.89)  LDD2107  [2]
 LDCM0515  Nucleophilic fragment 20b MDA-MB-231 C584(0.51); C564(0.88)  LDD2108  [2]
 LDCM0516  Nucleophilic fragment 21a MDA-MB-231 C584(0.56); C564(0.97)  LDD2109  [2]
 LDCM0517  Nucleophilic fragment 21b MDA-MB-231 C584(1.86)  LDD2110  [2]
 LDCM0518  Nucleophilic fragment 22a MDA-MB-231 C221(0.76); C584(0.67)  LDD2111  [2]
 LDCM0521  Nucleophilic fragment 23b MDA-MB-231 C221(0.99)  LDD2114  [2]
 LDCM0523  Nucleophilic fragment 24b MDA-MB-231 C584(0.92)  LDD2116  [2]
 LDCM0525  Nucleophilic fragment 25b MDA-MB-231 C584(0.94); C564(1.19)  LDD2118  [2]
 LDCM0527  Nucleophilic fragment 26b MDA-MB-231 C584(0.74)  LDD2120  [2]
 LDCM0529  Nucleophilic fragment 27b MDA-MB-231 C584(0.74)  LDD2122  [2]
 LDCM0530  Nucleophilic fragment 28a MDA-MB-231 C221(0.72); C584(0.75)  LDD2123  [2]
 LDCM0531  Nucleophilic fragment 28b MDA-MB-231 C584(0.90)  LDD2124  [2]
 LDCM0532  Nucleophilic fragment 29a MDA-MB-231 C584(0.49); C564(0.46)  LDD2125  [2]
 LDCM0533  Nucleophilic fragment 29b MDA-MB-231 C584(0.96)  LDD2126  [2]
 LDCM0534  Nucleophilic fragment 30a MDA-MB-231 C221(0.81); C584(0.66)  LDD2127  [2]
 LDCM0535  Nucleophilic fragment 30b MDA-MB-231 C584(0.81)  LDD2128  [2]
 LDCM0536  Nucleophilic fragment 31 MDA-MB-231 C221(0.91); C584(0.74); C564(0.65)  LDD2129  [2]
 LDCM0542  Nucleophilic fragment 37 MDA-MB-231 C221(0.71); C584(0.95)  LDD2135  [2]
 LDCM0543  Nucleophilic fragment 38 MDA-MB-231 C221(1.02); C584(0.84)  LDD2136  [2]
 LDCM0544  Nucleophilic fragment 39 MDA-MB-231 C221(0.85); C584(0.97)  LDD2137  [2]
 LDCM0211  Nucleophilic fragment 3b MDA-MB-231 C564(1.41); C584(1.02); C221(0.84)  LDD1700  [2]
 LDCM0546  Nucleophilic fragment 40 MDA-MB-231 C221(0.58); C584(0.52)  LDD2140  [2]
 LDCM0547  Nucleophilic fragment 41 MDA-MB-231 C221(0.46); C584(0.49); C564(0.77)  LDD2141  [2]
 LDCM0549  Nucleophilic fragment 43 MDA-MB-231 C584(1.11)  LDD2143  [2]
 LDCM0550  Nucleophilic fragment 5a MDA-MB-231 C221(1.98); C584(1.71); C796(1.45)  LDD2144  [2]
 LDCM0551  Nucleophilic fragment 5b MDA-MB-231 C584(3.09)  LDD2145  [2]
 LDCM0552  Nucleophilic fragment 6a MDA-MB-231 C221(0.53); C584(0.71); C796(0.60)  LDD2146  [2]
 LDCM0555  Nucleophilic fragment 7b MDA-MB-231 C584(0.87)  LDD2149  [2]
 LDCM0556  Nucleophilic fragment 8a MDA-MB-231 C584(0.27)  LDD2150  [2]
 LDCM0557  Nucleophilic fragment 8b MDA-MB-231 C584(1.08)  LDD2151  [2]
 LDCM0559  Nucleophilic fragment 9b MDA-MB-231 C221(2.08); C584(1.68)  LDD2153  [2]
 LDCM0131  RA190 MM1.R C221(1.34)  LDD0304  [19]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Serine/threonine-protein kinase PLK4 (PLK4) Ser/Thr protein kinase family O00444
Kinesin-like protein KIF23 (KIF23) Kinesin family Q02241
Transporter and channel
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Cadherin-1 (CDH1) . P12830
Other
Click To Hide/Show 3 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Catenin alpha-1 (CTNNA1) Vinculin/alpha-catenin family P35221
Rac GTPase-activating protein 1 (RACGAP1) . Q9H0H5
SH2/SH3 adapter protein NCK1 (NCK1) . P16333

References

1 Quantitative and Site-Specific Chemoproteomic Profiling of Targets of Acrolein. Chem Res Toxicol. 2019 Mar 18;32(3):467-473. doi: 10.1021/acs.chemrestox.8b00343. Epub 2019 Jan 15.
2 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
3 Chemoproteomic capture of RNA binding activity in living cells. Nat Commun. 2023 Oct 7;14(1):6282. doi: 10.1038/s41467-023-41844-z.
Mass spectrometry data entry: PXD044625
4 Covalent Inhibition by a Natural Product-Inspired Latent Electrophile. J Am Chem Soc. 2023 May 24;145(20):11097-11109. doi: 10.1021/jacs.3c00598. Epub 2023 May 15.
5 Targeted Proteomic Approaches for Proteome-Wide Characterizations of the AMP-Binding Capacities of Kinases. J Proteome Res. 2022 Aug 5;21(8):2063-2070. doi: 10.1021/acs.jproteome.2c00225. Epub 2022 Jul 12.
6 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
7 SP3-Enabled Rapid and High Coverage Chemoproteomic Identification of Cell-State-Dependent Redox-Sensitive Cysteines. Mol Cell Proteomics. 2022 Apr;21(4):100218. doi: 10.1016/j.mcpro.2022.100218. Epub 2022 Feb 25.
Mass spectrometry data entry: PXD029500 , PXD031647
8 Multiplexed CuAAC Suzuki-Miyaura Labeling for Tandem Activity-Based Chemoproteomic Profiling. Anal Chem. 2021 Feb 2;93(4):2610-2618. doi: 10.1021/acs.analchem.0c04726. Epub 2021 Jan 20.
Mass spectrometry data entry: PXD022279
9 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
10 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
11 DFT-Guided Discovery of Ethynyl-Triazolyl-Phosphinates as Modular Electrophiles for Chemoselective Cysteine Bioconjugation and Profiling. Angew Chem Int Ed Engl. 2022 Oct 10;61(41):e202205348. doi: 10.1002/anie.202205348. Epub 2022 Aug 22.
Mass spectrometry data entry: PXD033004
12 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
13 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
14 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
15 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
16 Covalent Ligand Screening Uncovers a RNF4 E3 Ligase Recruiter for Targeted Protein Degradation Applications. ACS Chem Biol. 2019 Nov 15;14(11):2430-2440. doi: 10.1021/acschembio.8b01083. Epub 2019 May 13.
17 Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry. Methods Enzymol. 2023;679:295-322. doi: 10.1016/bs.mie.2022.07.037. Epub 2022 Sep 7.
Mass spectrometry data entry: PXD027578
18 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
19 Physical and Functional Analysis of the Putative Rpn13 Inhibitor RA190. Cell Chem Biol. 2020 Nov 19;27(11):1371-1382.e6. doi: 10.1016/j.chembiol.2020.08.007. Epub 2020 Aug 27.