Details of the Target

General Information of Target

Target ID LDTP11232
Target Name Iron-sulfur cluster assembly 1 homolog, mitochondrial (ISCA1)
Gene Name ISCA1
Gene ID 81689
Synonyms
HBLD2; Iron-sulfur cluster assembly 1 homolog, mitochondrial; HESB-like domain-containing protein 2; Iron-sulfur assembly protein IscA; hIscA
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MEAPPVTMMPVTGGTINMMEYLLQGSVLDHSLESLIHRLRGLCDNMEPETFLDHEMVFLL
KGQQASPFVLRARRSMDRAGAPWHLRYLGQPEMGDKNRHALVRNCVDIATSENLTDFLME
MGFRMDHEFVAKGHLFRKGIMKIMVYKIFRILVPGNTDSTEALSLSYLVELSVVAPAGQD
MVSDDMKNFAEQLKPLVHLEKIDPKRLM
Target Bioclass
Other
Family
HesB/IscA family
Subcellular location
Mitochondrion
Function Involved in the maturation of mitochondrial 4Fe-4S proteins functioning late in the iron-sulfur cluster assembly pathway. Probably involved in the binding of an intermediate of Fe/S cluster assembly.
Uniprot ID
Q9BUE6
Ensemble ID
ENST00000375991.9
HGNC ID
HGNC:28660

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
HT115 SNV: p.T67A .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 4 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C57(1.36)  LDD0080  [1]
TFBX
 Probe Info  		C57(0.00); C123(0.00); C121(0.00)  LDD0027  [2]
IPM
 Probe Info  		N.A.  LDD0147  [2]
NAIA_5
 Probe Info  		N.A.  LDD2223  [3]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0216  AC100 HCT 116 C121(1.11)  LDD0533  [1]
 LDCM0217  AC101 HCT 116 C121(1.11)  LDD0534  [1]
 LDCM0218  AC102 HCT 116 C121(1.11)  LDD0535  [1]
 LDCM0219  AC103 HCT 116 C121(1.10)  LDD0536  [1]
 LDCM0220  AC104 HCT 116 C121(1.03)  LDD0537  [1]
 LDCM0221  AC105 HCT 116 C121(1.22)  LDD0538  [1]
 LDCM0222  AC106 HCT 116 C121(1.18)  LDD0539  [1]
 LDCM0223  AC107 HCT 116 C121(1.11)  LDD0540  [1]
 LDCM0224  AC108 HCT 116 C121(1.05)  LDD0541  [1]
 LDCM0225  AC109 HCT 116 C121(1.13)  LDD0542  [1]
 LDCM0227  AC110 HCT 116 C121(1.19)  LDD0544  [1]
 LDCM0228  AC111 HCT 116 C121(1.15)  LDD0545  [1]
 LDCM0229  AC112 HCT 116 C121(1.30)  LDD0546  [1]
 LDCM0230  AC113 HCT 116 C121(0.86)  LDD0547  [1]
 LDCM0231  AC114 HCT 116 C121(0.85)  LDD0548  [1]
 LDCM0232  AC115 HCT 116 C121(0.96)  LDD0549  [1]
 LDCM0233  AC116 HCT 116 C121(1.71)  LDD0550  [1]
 LDCM0234  AC117 HCT 116 C121(0.77)  LDD0551  [1]
 LDCM0235  AC118 HCT 116 C121(0.99)  LDD0552  [1]
 LDCM0236  AC119 HCT 116 C121(0.91)  LDD0553  [1]
 LDCM0238  AC120 HCT 116 C121(1.27)  LDD0555  [1]
 LDCM0239  AC121 HCT 116 C121(0.76)  LDD0556  [1]
 LDCM0240  AC122 HCT 116 C121(1.01)  LDD0557  [1]
 LDCM0241  AC123 HCT 116 C121(0.62)  LDD0558  [1]
 LDCM0242  AC124 HCT 116 C121(1.22)  LDD0559  [1]
 LDCM0243  AC125 HCT 116 C121(1.15)  LDD0560  [1]
 LDCM0244  AC126 HCT 116 C121(1.17)  LDD0561  [1]
 LDCM0245  AC127 HCT 116 C121(1.10)  LDD0562  [1]
 LDCM0246  AC128 HEK-293T C123(1.00)  LDD0844  [1]
 LDCM0247  AC129 HEK-293T C123(1.08)  LDD0845  [1]
 LDCM0249  AC130 HEK-293T C123(1.31)  LDD0847  [1]
 LDCM0250  AC131 HEK-293T C123(1.25)  LDD0848  [1]
 LDCM0251  AC132 HEK-293T C123(1.06)  LDD0849  [1]
 LDCM0252  AC133 HEK-293T C123(1.36)  LDD0850  [1]
 LDCM0253  AC134 HEK-293T C123(1.04)  LDD0851  [1]
 LDCM0254  AC135 HEK-293T C123(1.33)  LDD0852  [1]
 LDCM0255  AC136 HEK-293T C123(0.90)  LDD0853  [1]
 LDCM0256  AC137 HEK-293T C123(1.08)  LDD0854  [1]
 LDCM0257  AC138 HEK-293T C123(1.04)  LDD0855  [1]
 LDCM0258  AC139 HEK-293T C123(1.06)  LDD0856  [1]
 LDCM0260  AC140 HEK-293T C123(1.12)  LDD0858  [1]
 LDCM0261  AC141 HEK-293T C123(1.03)  LDD0859  [1]
 LDCM0262  AC142 HEK-293T C123(1.27)  LDD0860  [1]
 LDCM0276  AC17 HCT 116 C121(1.09)  LDD0593  [1]
 LDCM0277  AC18 HCT 116 C121(1.43)  LDD0594  [1]
 LDCM0278  AC19 HCT 116 C121(0.98)  LDD0595  [1]
 LDCM0280  AC20 HCT 116 C121(1.13)  LDD0597  [1]
 LDCM0281  AC21 HCT 116 C121(0.96)  LDD0598  [1]
 LDCM0282  AC22 HCT 116 C121(1.26)  LDD0599  [1]
 LDCM0283  AC23 HCT 116 C121(1.16)  LDD0600  [1]
 LDCM0284  AC24 HCT 116 C121(0.90)  LDD0601  [1]
 LDCM0285  AC25 HCT 116 C121(1.12)  LDD0602  [1]
 LDCM0286  AC26 HCT 116 C121(1.13)  LDD0603  [1]
 LDCM0287  AC27 HCT 116 C121(1.17)  LDD0604  [1]
 LDCM0288  AC28 HCT 116 C121(1.07)  LDD0605  [1]
 LDCM0289  AC29 HCT 116 C121(1.35)  LDD0606  [1]
 LDCM0291  AC30 HCT 116 C121(1.17)  LDD0608  [1]
 LDCM0292  AC31 HCT 116 C121(1.25)  LDD0609  [1]
 LDCM0293  AC32 HCT 116 C121(1.51)  LDD0610  [1]
 LDCM0294  AC33 HCT 116 C121(1.28)  LDD0611  [1]
 LDCM0295  AC34 HCT 116 C121(1.21)  LDD0612  [1]
 LDCM0296  AC35 HCT 116 C121(0.92)  LDD0613  [1]
 LDCM0297  AC36 HCT 116 C121(0.98)  LDD0614  [1]
 LDCM0298  AC37 HCT 116 C121(0.80)  LDD0615  [1]
 LDCM0299  AC38 HCT 116 C121(1.07)  LDD0616  [1]
 LDCM0300  AC39 HCT 116 C121(1.05)  LDD0617  [1]
 LDCM0302  AC40 HCT 116 C121(1.32)  LDD0619  [1]
 LDCM0303  AC41 HCT 116 C121(1.11)  LDD0620  [1]
 LDCM0304  AC42 HCT 116 C121(1.22)  LDD0621  [1]
 LDCM0305  AC43 HCT 116 C121(1.39)  LDD0622  [1]
 LDCM0306  AC44 HCT 116 C121(1.19)  LDD0623  [1]
 LDCM0307  AC45 HCT 116 C121(1.23)  LDD0624  [1]
 LDCM0308  AC46 HCT 116 C121(0.87)  LDD0625  [1]
 LDCM0309  AC47 HCT 116 C121(1.17)  LDD0626  [1]
 LDCM0310  AC48 HCT 116 C121(1.72)  LDD0627  [1]
 LDCM0311  AC49 HCT 116 C121(1.61)  LDD0628  [1]
 LDCM0313  AC50 HCT 116 C121(1.41)  LDD0630  [1]
 LDCM0314  AC51 HCT 116 C121(0.81)  LDD0631  [1]
 LDCM0315  AC52 HCT 116 C121(1.20)  LDD0632  [1]
 LDCM0316  AC53 HCT 116 C121(1.28)  LDD0633  [1]
 LDCM0317  AC54 HCT 116 C121(1.16)  LDD0634  [1]
 LDCM0318  AC55 HCT 116 C121(1.49)  LDD0635  [1]
 LDCM0319  AC56 HCT 116 C121(1.46)  LDD0636  [1]
 LDCM0320  AC57 HEK-293T C123(1.18)  LDD0918  [1]
 LDCM0321  AC58 HEK-293T C123(0.89)  LDD0919  [1]
 LDCM0322  AC59 HEK-293T C123(1.14)  LDD0920  [1]
 LDCM0324  AC60 HEK-293T C123(1.25)  LDD0922  [1]
 LDCM0325  AC61 HEK-293T C123(1.25)  LDD0923  [1]
 LDCM0326  AC62 HEK-293T C123(1.37)  LDD0924  [1]
 LDCM0327  AC63 HEK-293T C123(1.14)  LDD0925  [1]
 LDCM0328  AC64 HEK-293T C123(1.26)  LDD0926  [1]
 LDCM0329  AC65 HEK-293T C123(1.16)  LDD0927  [1]
 LDCM0330  AC66 HEK-293T C123(1.36)  LDD0928  [1]
 LDCM0331  AC67 HEK-293T C123(1.05)  LDD0929  [1]
 LDCM0332  AC68 HEK-293T C123(0.96)  LDD0930  [1]
 LDCM0333  AC69 HEK-293T C123(0.92)  LDD0931  [1]
 LDCM0335  AC70 HEK-293T C123(0.96)  LDD0933  [1]
 LDCM0336  AC71 HEK-293T C123(1.06)  LDD0934  [1]
 LDCM0337  AC72 HEK-293T C123(1.26)  LDD0935  [1]
 LDCM0338  AC73 HEK-293T C123(1.10)  LDD0936  [1]
 LDCM0339  AC74 HEK-293T C123(0.83)  LDD0937  [1]
 LDCM0340  AC75 HEK-293T C123(1.15)  LDD0938  [1]
 LDCM0341  AC76 HEK-293T C123(0.88)  LDD0939  [1]
 LDCM0342  AC77 HEK-293T C123(1.07)  LDD0940  [1]
 LDCM0343  AC78 HEK-293T C123(0.93)  LDD0941  [1]
 LDCM0344  AC79 HEK-293T C123(0.88)  LDD0942  [1]
 LDCM0346  AC80 HEK-293T C123(0.82)  LDD0944  [1]
 LDCM0347  AC81 HEK-293T C123(0.85)  LDD0945  [1]
 LDCM0348  AC82 HEK-293T C123(0.92)  LDD0946  [1]
 LDCM0349  AC83 HEK-293T C123(1.04)  LDD0947  [1]
 LDCM0350  AC84 HEK-293T C123(1.31)  LDD0948  [1]
 LDCM0351  AC85 HEK-293T C123(1.15)  LDD0949  [1]
 LDCM0352  AC86 HEK-293T C123(1.08)  LDD0950  [1]
 LDCM0353  AC87 HEK-293T C123(0.97)  LDD0951  [1]
 LDCM0354  AC88 HEK-293T C123(1.10)  LDD0952  [1]
 LDCM0355  AC89 HEK-293T C123(1.15)  LDD0953  [1]
 LDCM0357  AC90 HEK-293T C123(0.91)  LDD0955  [1]
 LDCM0358  AC91 HEK-293T C123(0.69)  LDD0956  [1]
 LDCM0359  AC92 HEK-293T C123(1.06)  LDD0957  [1]
 LDCM0360  AC93 HEK-293T C123(0.99)  LDD0958  [1]
 LDCM0361  AC94 HEK-293T C123(0.87)  LDD0959  [1]
 LDCM0362  AC95 HEK-293T C123(1.11)  LDD0960  [1]
 LDCM0363  AC96 HEK-293T C123(0.65)  LDD0961  [1]
 LDCM0364  AC97 HEK-293T C123(0.90)  LDD0962  [1]
 LDCM0365  AC98 HCT 116 C121(1.18)  LDD0682  [1]
 LDCM0366  AC99 HCT 116 C121(1.26)  LDD0683  [1]
 LDCM0374  CL105 HCT 116 C121(1.17)  LDD0691  [1]
 LDCM0375  CL106 HCT 116 C121(1.95)  LDD0692  [1]
 LDCM0376  CL107 HCT 116 C121(1.49)  LDD0693  [1]
 LDCM0377  CL108 HCT 116 C121(1.73)  LDD0694  [1]
 LDCM0378  CL109 HCT 116 C121(1.09)  LDD0695  [1]
 LDCM0380  CL110 HCT 116 C121(1.60)  LDD0697  [1]
 LDCM0381  CL111 HCT 116 C121(1.03)  LDD0698  [1]
 LDCM0382  CL112 HCT 116 C121(0.94)  LDD0699  [1]
 LDCM0383  CL113 HCT 116 C121(1.27)  LDD0700  [1]
 LDCM0384  CL114 HCT 116 C121(1.34)  LDD0701  [1]
 LDCM0385  CL115 HCT 116 C121(1.21)  LDD0702  [1]
 LDCM0386  CL116 HCT 116 C121(1.28)  LDD0703  [1]
 LDCM0387  CL117 HCT 116 C121(1.69)  LDD0704  [1]
 LDCM0388  CL118 HCT 116 C121(1.53)  LDD0705  [1]
 LDCM0389  CL119 HCT 116 C121(1.14)  LDD0706  [1]
 LDCM0391  CL120 HCT 116 C121(1.08)  LDD0708  [1]
 LDCM0392  CL121 HCT 116 C121(0.68)  LDD0709  [1]
 LDCM0393  CL122 HCT 116 C121(1.45)  LDD0710  [1]
 LDCM0394  CL123 HCT 116 C121(1.27)  LDD0711  [1]
 LDCM0395  CL124 HCT 116 C121(1.38)  LDD0712  [1]
 LDCM0396  CL125 HEK-293T C123(1.04)  LDD0994  [1]
 LDCM0397  CL126 HEK-293T C123(1.48)  LDD0995  [1]
 LDCM0398  CL127 HEK-293T C123(0.97)  LDD0996  [1]
 LDCM0399  CL128 HEK-293T C123(1.36)  LDD0997  [1]
 LDCM0420  CL31 HCT 116 C121(0.76)  LDD0737  [1]
 LDCM0421  CL32 HCT 116 C121(0.85)  LDD0738  [1]
 LDCM0422  CL33 HCT 116 C121(0.70)  LDD0739  [1]
 LDCM0423  CL34 HCT 116 C121(0.79)  LDD0740  [1]
 LDCM0424  CL35 HCT 116 C121(0.83)  LDD0741  [1]
 LDCM0425  CL36 HCT 116 C121(0.81)  LDD0742  [1]
 LDCM0426  CL37 HCT 116 C121(0.78)  LDD0743  [1]
 LDCM0428  CL39 HCT 116 C121(0.83)  LDD0745  [1]
 LDCM0430  CL40 HCT 116 C121(0.95)  LDD0747  [1]
 LDCM0431  CL41 HCT 116 C121(0.85)  LDD0748  [1]
 LDCM0432  CL42 HCT 116 C121(0.85)  LDD0749  [1]
 LDCM0433  CL43 HCT 116 C121(0.76)  LDD0750  [1]
 LDCM0434  CL44 HCT 116 C121(0.90)  LDD0751  [1]
 LDCM0435  CL45 HCT 116 C121(0.82)  LDD0752  [1]
 LDCM0453  CL61 HCT 116 C121(1.03)  LDD0770  [1]
 LDCM0454  CL62 HCT 116 C121(1.30)  LDD0771  [1]
 LDCM0455  CL63 HCT 116 C121(1.38)  LDD0772  [1]
 LDCM0456  CL64 HCT 116 C121(1.40)  LDD0773  [1]
 LDCM0457  CL65 HCT 116 C121(1.42)  LDD0774  [1]
 LDCM0458  CL66 HCT 116 C121(1.79)  LDD0775  [1]
 LDCM0459  CL67 HCT 116 C121(1.88)  LDD0776  [1]
 LDCM0460  CL68 HCT 116 C121(1.40)  LDD0777  [1]
 LDCM0461  CL69 HCT 116 C121(1.30)  LDD0778  [1]
 LDCM0463  CL70 HCT 116 C121(1.30)  LDD0780  [1]
 LDCM0464  CL71 HCT 116 C121(1.17)  LDD0781  [1]
 LDCM0465  CL72 HCT 116 C121(1.15)  LDD0782  [1]
 LDCM0466  CL73 HCT 116 C121(1.36)  LDD0783  [1]
 LDCM0467  CL74 HCT 116 C121(1.42)  LDD0784  [1]
 LDCM0469  CL76 HCT 116 C121(0.94)  LDD0786  [1]
 LDCM0470  CL77 HCT 116 C121(1.09)  LDD0787  [1]
 LDCM0471  CL78 HCT 116 C121(0.99)  LDD0788  [1]
 LDCM0472  CL79 HCT 116 C121(1.49)  LDD0789  [1]
 LDCM0474  CL80 HCT 116 C121(1.22)  LDD0791  [1]
 LDCM0475  CL81 HCT 116 C121(1.02)  LDD0792  [1]
 LDCM0476  CL82 HCT 116 C121(0.79)  LDD0793  [1]
 LDCM0477  CL83 HCT 116 C121(1.14)  LDD0794  [1]
 LDCM0478  CL84 HCT 116 C121(1.28)  LDD0795  [1]
 LDCM0479  CL85 HCT 116 C121(0.95)  LDD0796  [1]
 LDCM0480  CL86 HCT 116 C121(1.01)  LDD0797  [1]
 LDCM0481  CL87 HCT 116 C121(1.08)  LDD0798  [1]
 LDCM0482  CL88 HCT 116 C121(1.00)  LDD0799  [1]
 LDCM0483  CL89 HCT 116 C121(1.09)  LDD0800  [1]
 LDCM0485  CL90 HCT 116 C121(1.06)  LDD0802  [1]
 LDCM0468  Fragment33 HCT 116 C121(1.25)  LDD0785  [1]
 LDCM0427  Fragment51 HCT 116 C121(1.13)  LDD0744  [1]
 LDCM0022  KB02 HCT 116 C57(1.36)  LDD0080  [1]
 LDCM0023  KB03 HCT 116 C57(1.40)  LDD0081  [1]
 LDCM0024  KB05 HCT 116 C57(1.35)  LDD0082  [1]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Other
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Iron-sulfur cluster assembly 2 homolog, mitochondrial (ISCA2) HesB/IscA family Q86U28

References

1 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
2 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
3 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264