Details of the Target

General Information of Target

Target ID LDTP09764
Target Name Acid sphingomyelinase-like phosphodiesterase 3b (SMPDL3B)
Gene Name SMPDL3B
Gene ID 27293
Synonyms
ASML3B; ASMLPD; Acid sphingomyelinase-like phosphodiesterase 3b; ASM-like phosphodiesterase 3b; EC 3.1.4.-
3D Structure
Download
2D Sequence (FASTA)
Download
3D Structure (PDB)
Download
Sequence
MALVRALVCCLLTAWHCRSGLGLPVAPAGGRNPPPAIGQFWHVTDLHLDPTYHITDDHTK
VCASSKGANASNPGPFGDVLCDSPYQLILSAFDFIKNSGQEASFMIWTGDSPPHVPVPEL
STDTVINVITNMTTTIQSLFPNLQVFPALGNHDYWPQDQLPVVTSKVYNAVANLWKPWLD
EEAISTLRKGGFYSQKVTTNPNLRIISLNTNLYYGPNIMTLNKTDPANQFEWLESTLNNS
QQNKEKVYIIAHVPVGYLPSSQNITAMREYYNEKLIDIFQKYSDVIAGQFYGHTHRDSIM
VLSDKKGSPVNSLFVAPAVTPVKSVLEKQTNNPGIRLFQYDPRDYKLLDMLQYYLNLTEA
NLKGESIWKLEYILTQTYDIEDLQPESLYGLAKQFTILDSKQFIKYYNYFFVSYDSSVTC
DKTCKAFQICAIMNLDNISYADCLKQLYIKHNY
Target Bioclass
Enzyme
Family
Acid sphingomyelinase family
Subcellular location
Secreted
Function
Lipid-modulating phosphodiesterase. Active on the surface of macrophages and dendritic cells and strongly influences macrophage lipid composition and membrane fluidity. Acts as a negative regulator of Toll-like receptor signaling. Has in vitro phosphodiesterase activity, but the physiological substrate is unknown. Lacks activity with phosphocholine-containing lipids, but can cleave CDP-choline, and can release phosphate from ATP and ADP (in vitro).
Uniprot ID
Q92485
Ensemble ID
ENST00000373888.8
HGNC ID
HGNC:21416

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
CAL62 SNV: p.G133Ter .
DU145 SNV: p.S397P .
MEWO SNV: p.P67L .
MFE319 SNV: p.T427N .
TCCSUP SNV: p.P441R .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 1 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C177(1.74)  LDD0532  [1]
PAL-AfBPP Probe
Click To Hide/Show 2 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C170
 Probe Info  		14.93  LDD1850  [2]
C171
 Probe Info  		6.28  LDD1851  [2]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0214  AC1 HEK-293T C177(0.89)  LDD1507  [3]
 LDCM0215  AC10 HCT 116 C177(1.74)  LDD0532  [1]
 LDCM0216  AC100 HCT 116 C177(0.86)  LDD0533  [1]
 LDCM0217  AC101 HCT 116 C177(1.09)  LDD0534  [1]
 LDCM0218  AC102 HCT 116 C177(0.93)  LDD0535  [1]
 LDCM0219  AC103 HCT 116 C177(0.98)  LDD0536  [1]
 LDCM0220  AC104 HCT 116 C177(0.80)  LDD0537  [1]
 LDCM0221  AC105 HCT 116 C177(1.08)  LDD0538  [1]
 LDCM0222  AC106 HCT 116 C177(0.90)  LDD0539  [1]
 LDCM0223  AC107 HCT 116 C177(1.06)  LDD0540  [1]
 LDCM0224  AC108 HCT 116 C177(1.07)  LDD0541  [1]
 LDCM0225  AC109 HCT 116 C177(0.88)  LDD0542  [1]
 LDCM0226  AC11 HCT 116 C177(1.11)  LDD0543  [1]
 LDCM0227  AC110 HCT 116 C177(0.89)  LDD0544  [1]
 LDCM0228  AC111 HCT 116 C177(0.98)  LDD0545  [1]
 LDCM0229  AC112 HCT 116 C177(1.07)  LDD0546  [1]
 LDCM0237  AC12 HCT 116 C177(0.97)  LDD0554  [1]
 LDCM0259  AC14 HCT 116 C177(1.34)  LDD0576  [1]
 LDCM0270  AC15 HCT 116 C177(1.56)  LDD0587  [1]
 LDCM0276  AC17 HEK-293T C177(0.98)  LDD1515  [3]
 LDCM0280  AC20 HEK-293T C177(1.04)  LDD1519  [3]
 LDCM0282  AC22 HEK-293T C177(1.09)  LDD1521  [3]
 LDCM0284  AC24 HEK-293T C177(1.10)  LDD1523  [3]
 LDCM0285  AC25 HEK-293T C177(0.89)  LDD1524  [3]
 LDCM0288  AC28 HEK-293T C177(0.94)  LDD1527  [3]
 LDCM0291  AC30 HEK-293T C177(0.94)  LDD1530  [3]
 LDCM0293  AC32 HEK-293T C177(1.08)  LDD1532  [3]
 LDCM0294  AC33 HEK-293T C177(1.11)  LDD1533  [3]
 LDCM0297  AC36 HEK-293T C177(1.17)  LDD1536  [3]
 LDCM0299  AC38 HEK-293T C177(1.04)  LDD1538  [3]
 LDCM0301  AC4 HEK-293T C177(1.02)  LDD1540  [3]
 LDCM0302  AC40 HEK-293T C177(1.00)  LDD1541  [3]
 LDCM0303  AC41 HEK-293T C177(1.10)  LDD1542  [3]
 LDCM0306  AC44 HEK-293T C177(1.21)  LDD1545  [3]
 LDCM0308  AC46 HEK-293T C177(1.00)  LDD1547  [3]
 LDCM0310  AC48 HEK-293T C177(1.12)  LDD1549  [3]
 LDCM0311  AC49 HEK-293T C177(1.00)  LDD1550  [3]
 LDCM0315  AC52 HEK-293T C177(1.20)  LDD1554  [3]
 LDCM0317  AC54 HEK-293T C177(1.08)  LDD1556  [3]
 LDCM0319  AC56 HEK-293T C177(1.13)  LDD1558  [3]
 LDCM0320  AC57 HEK-293T C177(0.95)  LDD1559  [3]
 LDCM0323  AC6 HCT 116 C177(1.27)  LDD0640  [1]
 LDCM0324  AC60 HEK-293T C177(1.08)  LDD1563  [3]
 LDCM0326  AC62 HEK-293T C177(1.04)  LDD1565  [3]
 LDCM0328  AC64 HEK-293T C177(0.98)  LDD1567  [3]
 LDCM0334  AC7 HCT 116 C177(1.00)  LDD0651  [1]
 LDCM0345  AC8 HCT 116 C177(1.00)  LDD0662  [1]
 LDCM0365  AC98 HCT 116 C177(1.14)  LDD0682  [1]
 LDCM0366  AC99 HCT 116 C177(0.79)  LDD0683  [1]
 LDCM0248  AKOS034007472 HCT 116 C177(1.13)  LDD0565  [1]
 LDCM0356  AKOS034007680 HCT 116 C177(1.38)  LDD0673  [1]
 LDCM0275  AKOS034007705 HCT 116 C177(2.21)  LDD0592  [1]
 LDCM0367  CL1 HEK-293T C177(0.95)  LDD1571  [3]
 LDCM0368  CL10 HEK-293T C177(0.89)  LDD1572  [3]
 LDCM0370  CL101 HCT 116 C177(1.10)  LDD0687  [1]
 LDCM0371  CL102 HCT 116 C177(0.96)  LDD0688  [1]
 LDCM0372  CL103 HCT 116 C177(0.80)  LDD0689  [1]
 LDCM0373  CL104 HCT 116 C177(1.15)  LDD0690  [1]
 LDCM0374  CL105 HEK-293T C177(1.06)  LDD1578  [3]
 LDCM0378  CL109 HEK-293T C177(0.97)  LDD1582  [3]
 LDCM0383  CL113 HEK-293T C177(1.03)  LDD1587  [3]
 LDCM0387  CL117 HEK-293T C177(1.07)  LDD1591  [3]
 LDCM0390  CL12 HEK-293T C177(0.93)  LDD1594  [3]
 LDCM0392  CL121 HEK-293T C177(1.04)  LDD1596  [3]
 LDCM0396  CL125 HEK-293T C177(1.02)  LDD1600  [3]
 LDCM0400  CL13 HEK-293T C177(0.91)  LDD1604  [3]
 LDCM0404  CL17 HEK-293T C177(0.95)  LDD1608  [3]
 LDCM0408  CL20 HEK-293T C177(1.10)  LDD1612  [3]
 LDCM0410  CL22 HEK-293T C177(0.95)  LDD1614  [3]
 LDCM0412  CL24 HEK-293T C177(1.04)  LDD1616  [3]
 LDCM0413  CL25 HEK-293T C177(1.09)  LDD1617  [3]
 LDCM0417  CL29 HEK-293T C177(1.01)  LDD1621  [3]
 LDCM0420  CL31 HCT 116 C177(0.81)  LDD0737  [1]
 LDCM0421  CL32 HCT 116 C177(0.96)  LDD0738  [1]
 LDCM0422  CL33 HCT 116 C177(0.71)  LDD0739  [1]
 LDCM0423  CL34 HCT 116 C177(1.00)  LDD0740  [1]
 LDCM0424  CL35 HCT 116 C177(0.88)  LDD0741  [1]
 LDCM0425  CL36 HCT 116 C177(0.80)  LDD0742  [1]
 LDCM0426  CL37 HCT 116 C177(0.98)  LDD0743  [1]
 LDCM0428  CL39 HCT 116 C177(1.17)  LDD0745  [1]
 LDCM0430  CL40 HCT 116 C177(0.94)  LDD0747  [1]
 LDCM0431  CL41 HCT 116 C177(1.25)  LDD0748  [1]
 LDCM0432  CL42 HCT 116 C177(1.53)  LDD0749  [1]
 LDCM0433  CL43 HCT 116 C177(1.01)  LDD0750  [1]
 LDCM0434  CL44 HCT 116 C177(0.87)  LDD0751  [1]
 LDCM0435  CL45 HCT 116 C177(1.02)  LDD0752  [1]
 LDCM0436  CL46 HCT 116 C177(0.99)  LDD0753  [1]
 LDCM0437  CL47 HCT 116 C177(0.89)  LDD0754  [1]
 LDCM0438  CL48 HCT 116 C177(0.74)  LDD0755  [1]
 LDCM0439  CL49 HCT 116 C177(0.61)  LDD0756  [1]
 LDCM0440  CL5 HEK-293T C177(0.95)  LDD1644  [3]
 LDCM0441  CL50 HCT 116 C177(0.94)  LDD0758  [1]
 LDCM0442  CL51 HCT 116 C177(0.85)  LDD0759  [1]
 LDCM0443  CL52 HCT 116 C177(0.78)  LDD0760  [1]
 LDCM0444  CL53 HCT 116 C177(0.77)  LDD0761  [1]
 LDCM0445  CL54 HCT 116 C177(0.81)  LDD0762  [1]
 LDCM0446  CL55 HCT 116 C177(0.78)  LDD0763  [1]
 LDCM0447  CL56 HCT 116 C177(1.06)  LDD0764  [1]
 LDCM0448  CL57 HCT 116 C177(0.88)  LDD0765  [1]
 LDCM0449  CL58 HCT 116 C177(0.93)  LDD0766  [1]
 LDCM0450  CL59 HCT 116 C177(0.93)  LDD0767  [1]
 LDCM0452  CL60 HCT 116 C177(0.94)  LDD0769  [1]
 LDCM0453  CL61 HCT 116 C177(1.26)  LDD0770  [1]
 LDCM0454  CL62 HCT 116 C177(1.30)  LDD0771  [1]
 LDCM0455  CL63 HCT 116 C177(1.14)  LDD0772  [1]
 LDCM0456  CL64 HCT 116 C177(1.24)  LDD0773  [1]
 LDCM0457  CL65 HCT 116 C177(1.24)  LDD0774  [1]
 LDCM0458  CL66 HCT 116 C177(1.88)  LDD0775  [1]
 LDCM0459  CL67 HCT 116 C177(1.57)  LDD0776  [1]
 LDCM0460  CL68 HCT 116 C177(1.62)  LDD0777  [1]
 LDCM0461  CL69 HCT 116 C177(1.10)  LDD0778  [1]
 LDCM0463  CL70 HCT 116 C177(1.23)  LDD0780  [1]
 LDCM0464  CL71 HCT 116 C177(1.39)  LDD0781  [1]
 LDCM0465  CL72 HCT 116 C177(1.49)  LDD0782  [1]
 LDCM0466  CL73 HCT 116 C177(1.33)  LDD0783  [1]
 LDCM0467  CL74 HCT 116 C177(1.57)  LDD0784  [1]
 LDCM0470  CL77 HEK-293T C177(0.90)  LDD1673  [3]
 LDCM0473  CL8 HEK-293T C177(0.86)  LDD1676  [3]
 LDCM0474  CL80 HEK-293T C177(1.13)  LDD1677  [3]
 LDCM0476  CL82 HEK-293T C177(0.98)  LDD1679  [3]
 LDCM0478  CL84 HEK-293T C177(0.96)  LDD1681  [3]
 LDCM0479  CL85 HEK-293T C177(1.09)  LDD1682  [3]
 LDCM0483  CL89 HEK-293T C177(1.13)  LDD1686  [3]
 LDCM0487  CL92 HEK-293T C177(1.19)  LDD1690  [3]
 LDCM0489  CL94 HEK-293T C177(1.05)  LDD1692  [3]
 LDCM0491  CL96 HEK-293T C177(0.98)  LDD1694  [3]
 LDCM0492  CL97 HEK-293T C177(0.92)  LDD1695  [3]
 LDCM0468  Fragment33 HCT 116 C177(1.44)  LDD0785  [1]
 LDCM0427  Fragment51 HCT 116 C177(1.20)  LDD0744  [1]
 LDCM0022  KB02 22RV1 C177(1.32)  LDD2243  [4]
 LDCM0023  KB03 22RV1 C177(1.57)  LDD2660  [4]
 LDCM0024  KB05 OVCAR-3 C177(1.10)  LDD3365  [4]

References

1 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
2 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587
3 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
4 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840