Details of the Target

General Information of Target

Target ID LDTP06641
Target Name Dual specificity protein phosphatase 6 (DUSP6)
Gene Name DUSP6
Gene ID 1848
Synonyms
MKP3; PYST1; Dual specificity protein phosphatase 6; EC 3.1.3.16; EC 3.1.3.48; Dual specificity protein phosphatase PYST1; Mitogen-activated protein kinase phosphatase 3; MAP kinase phosphatase 3; MKP-3
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MIDTLRPVPFASEMAISKTVAWLNEQLELGNERLLLMDCRPQELYESSHIESAINVAIPG
IMLRRLQKGNLPVRALFTRGEDRDRFTRRCGTDTVVLYDESSSDWNENTGGESVLGLLLK
KLKDEGCRAFYLEGGFSKFQAEFSLHCETNLDGSCSSSSPPLPVLGLGGLRISSDSSSDI
ESDLDRDPNSATDSDGSPLSNSQPSFPVEILPFLYLGCAKDSTNLDVLEEFGIKYILNVT
PNLPNLFENAGEFKYKQIPISDHWSQNLSQFFPEAISFIDEARGKNCGVLVHCLAGISRS
VTVTVAYLMQKLNLSMNDAYDIVKMKKSNISPNFNFMGQLLDFERTLGLSSPCDNRVPAQ
QLYFTTPSNQNVYQVDSLQST
Target Bioclass
Enzyme
Family
Protein-tyrosine phosphatase family, Non-receptor class dual specificity subfamily
Subcellular location
Cytoplasm
Function
Inactivates MAP kinases. Has a specificity for the ERK family. Plays an important role in alleviating chronic postoperative pain. Necessary for the normal dephosphorylation of the long-lasting phosphorylated forms of spinal MAPK1/3 and MAP kinase p38 induced by peripheral surgery, which drives the resolution of acute postoperative allodynia. Also important for dephosphorylation of MAPK1/3 in local wound tissue, which further contributes to resolution of acute pain. Promotes cell differentiation by regulating MAPK1/MAPK3 activity and regulating the expression of AP1 transcription factors.
Uniprot ID
Q16828
Ensemble ID
ENST00000279488.8
HGNC ID
HGNC:3072
ChEMBL ID
CHEMBL1250381

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
COLO792 SNV: p.S202R .
LNCaP clone FGC SNV: p.Y98Ter .
SNGM SNV: p.A129S .
SW1271 SNV: p.P59T .
UACC257 SNV: p.S300L .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 1 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C353(1.50)  LDD0532  [1]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0215  AC10 HCT 116 C353(1.50)  LDD0532  [1]
 LDCM0216  AC100 HCT 116 C353(0.84)  LDD0533  [1]
 LDCM0217  AC101 HCT 116 C353(1.22)  LDD0534  [1]
 LDCM0218  AC102 HCT 116 C353(1.27)  LDD0535  [1]
 LDCM0219  AC103 HCT 116 C353(1.36)  LDD0536  [1]
 LDCM0220  AC104 HCT 116 C353(1.05)  LDD0537  [1]
 LDCM0221  AC105 HCT 116 C353(1.59)  LDD0538  [1]
 LDCM0222  AC106 HCT 116 C353(1.49)  LDD0539  [1]
 LDCM0223  AC107 HCT 116 C353(1.81)  LDD0540  [1]
 LDCM0224  AC108 HCT 116 C353(0.85)  LDD0541  [1]
 LDCM0225  AC109 HCT 116 C353(1.13)  LDD0542  [1]
 LDCM0226  AC11 HCT 116 C353(1.38)  LDD0543  [1]
 LDCM0227  AC110 HCT 116 C353(0.90)  LDD0544  [1]
 LDCM0228  AC111 HCT 116 C353(1.03)  LDD0545  [1]
 LDCM0229  AC112 HCT 116 C353(1.56)  LDD0546  [1]
 LDCM0237  AC12 HCT 116 C353(1.36)  LDD0554  [1]
 LDCM0259  AC14 HCT 116 C353(1.31)  LDD0576  [1]
 LDCM0263  AC143 HCT 116 C353(1.61)  LDD0580  [1]
 LDCM0264  AC144 HCT 116 C353(2.17)  LDD0581  [1]
 LDCM0265  AC145 HCT 116 C353(1.57)  LDD0582  [1]
 LDCM0266  AC146 HCT 116 C353(1.74)  LDD0583  [1]
 LDCM0267  AC147 HCT 116 C353(1.91)  LDD0584  [1]
 LDCM0268  AC148 HCT 116 C353(2.01)  LDD0585  [1]
 LDCM0269  AC149 HCT 116 C353(1.99)  LDD0586  [1]
 LDCM0270  AC15 HCT 116 C353(1.46)  LDD0587  [1]
 LDCM0271  AC150 HCT 116 C353(1.89)  LDD0588  [1]
 LDCM0272  AC151 HCT 116 C353(1.29)  LDD0589  [1]
 LDCM0273  AC152 HCT 116 C353(1.76)  LDD0590  [1]
 LDCM0274  AC153 HCT 116 C353(2.31)  LDD0591  [1]
 LDCM0621  AC154 HCT 116 C353(2.06)  LDD2158  [1]
 LDCM0622  AC155 HCT 116 C353(1.76)  LDD2159  [1]
 LDCM0623  AC156 HCT 116 C353(1.40)  LDD2160  [1]
 LDCM0624  AC157 HCT 116 C353(1.77)  LDD2161  [1]
 LDCM0276  AC17 HCT 116 C353(0.83)  LDD0593  [1]
 LDCM0277  AC18 HCT 116 C353(0.65)  LDD0594  [1]
 LDCM0278  AC19 HCT 116 C353(0.84)  LDD0595  [1]
 LDCM0280  AC20 HCT 116 C353(0.96)  LDD0597  [1]
 LDCM0281  AC21 HCT 116 C353(0.93)  LDD0598  [1]
 LDCM0282  AC22 HCT 116 C353(0.80)  LDD0599  [1]
 LDCM0283  AC23 HCT 116 C353(1.01)  LDD0600  [1]
 LDCM0284  AC24 HCT 116 C353(0.94)  LDD0601  [1]
 LDCM0285  AC25 HCT 116 C353(1.75)  LDD0602  [1]
 LDCM0286  AC26 HCT 116 C353(1.23)  LDD0603  [1]
 LDCM0287  AC27 HCT 116 C353(1.41)  LDD0604  [1]
 LDCM0288  AC28 HCT 116 C353(1.42)  LDD0605  [1]
 LDCM0289  AC29 HCT 116 C353(1.25)  LDD0606  [1]
 LDCM0291  AC30 HCT 116 C353(1.83)  LDD0608  [1]
 LDCM0292  AC31 HCT 116 C353(2.43)  LDD0609  [1]
 LDCM0293  AC32 HCT 116 C353(2.09)  LDD0610  [1]
 LDCM0294  AC33 HCT 116 C353(2.14)  LDD0611  [1]
 LDCM0295  AC34 HCT 116 C353(2.19)  LDD0612  [1]
 LDCM0296  AC35 HCT 116 C353(0.97)  LDD0613  [1]
 LDCM0297  AC36 HCT 116 C353(0.87)  LDD0614  [1]
 LDCM0298  AC37 HCT 116 C353(1.13)  LDD0615  [1]
 LDCM0299  AC38 HCT 116 C353(0.91)  LDD0616  [1]
 LDCM0300  AC39 HCT 116 C353(1.97)  LDD0617  [1]
 LDCM0302  AC40 HCT 116 C353(1.64)  LDD0619  [1]
 LDCM0303  AC41 HCT 116 C353(1.24)  LDD0620  [1]
 LDCM0304  AC42 HCT 116 C353(1.31)  LDD0621  [1]
 LDCM0305  AC43 HCT 116 C353(1.13)  LDD0622  [1]
 LDCM0306  AC44 HCT 116 C353(1.18)  LDD0623  [1]
 LDCM0307  AC45 HCT 116 C353(1.81)  LDD0624  [1]
 LDCM0308  AC46 HCT 116 C353(1.04)  LDD0625  [1]
 LDCM0309  AC47 HCT 116 C353(1.56)  LDD0626  [1]
 LDCM0310  AC48 HCT 116 C353(1.46)  LDD0627  [1]
 LDCM0311  AC49 HCT 116 C353(1.82)  LDD0628  [1]
 LDCM0313  AC50 HCT 116 C353(1.83)  LDD0630  [1]
 LDCM0314  AC51 HCT 116 C353(0.87)  LDD0631  [1]
 LDCM0315  AC52 HCT 116 C353(1.34)  LDD0632  [1]
 LDCM0316  AC53 HCT 116 C353(1.85)  LDD0633  [1]
 LDCM0317  AC54 HCT 116 C353(1.62)  LDD0634  [1]
 LDCM0318  AC55 HCT 116 C353(1.88)  LDD0635  [1]
 LDCM0319  AC56 HCT 116 C353(2.13)  LDD0636  [1]
 LDCM0320  AC57 HCT 116 C353(0.76)  LDD0637  [1]
 LDCM0321  AC58 HCT 116 C353(0.94)  LDD0638  [1]
 LDCM0322  AC59 HCT 116 C353(1.55)  LDD0639  [1]
 LDCM0323  AC6 HCT 116 C353(1.30)  LDD0640  [1]
 LDCM0324  AC60 HCT 116 C353(1.37)  LDD0641  [1]
 LDCM0325  AC61 HCT 116 C353(0.85)  LDD0642  [1]
 LDCM0326  AC62 HCT 116 C353(1.41)  LDD0643  [1]
 LDCM0327  AC63 HCT 116 C353(0.88)  LDD0644  [1]
 LDCM0328  AC64 HCT 116 C353(1.15)  LDD0645  [1]
 LDCM0329  AC65 HCT 116 C353(0.56)  LDD0646  [1]
 LDCM0330  AC66 HCT 116 C353(0.64)  LDD0647  [1]
 LDCM0331  AC67 HCT 116 C353(0.73)  LDD0648  [1]
 LDCM0334  AC7 HCT 116 C353(1.41)  LDD0651  [1]
 LDCM0345  AC8 HCT 116 C353(1.20)  LDD0662  [1]
 LDCM0349  AC83 HCT 116 C353(2.23)  LDD0666  [1]
 LDCM0350  AC84 HCT 116 C353(2.18)  LDD0667  [1]
 LDCM0351  AC85 HCT 116 C353(1.23)  LDD0668  [1]
 LDCM0352  AC86 HCT 116 C353(1.14)  LDD0669  [1]
 LDCM0353  AC87 HCT 116 C353(1.01)  LDD0670  [1]
 LDCM0354  AC88 HCT 116 C353(1.56)  LDD0671  [1]
 LDCM0355  AC89 HCT 116 C353(1.84)  LDD0672  [1]
 LDCM0357  AC90 HCT 116 C353(0.88)  LDD0674  [1]
 LDCM0358  AC91 HCT 116 C353(2.14)  LDD0675  [1]
 LDCM0359  AC92 HCT 116 C353(2.31)  LDD0676  [1]
 LDCM0360  AC93 HCT 116 C353(1.18)  LDD0677  [1]
 LDCM0361  AC94 HCT 116 C353(1.08)  LDD0678  [1]
 LDCM0362  AC95 HCT 116 C353(1.20)  LDD0679  [1]
 LDCM0363  AC96 HCT 116 C353(1.78)  LDD0680  [1]
 LDCM0364  AC97 HCT 116 C353(2.07)  LDD0681  [1]
 LDCM0365  AC98 HCT 116 C353(2.01)  LDD0682  [1]
 LDCM0366  AC99 HCT 116 C353(1.19)  LDD0683  [1]
 LDCM0248  AKOS034007472 HCT 116 C353(1.25)  LDD0565  [1]
 LDCM0356  AKOS034007680 HCT 116 C353(1.40)  LDD0673  [1]
 LDCM0275  AKOS034007705 HCT 116 C353(2.24)  LDD0592  [1]
 LDCM0020  ARS-1620 HCC44 C353(1.19)  LDD2171  [1]
 LDCM0367  CL1 HCT 116 C353(1.43)  LDD0684  [1]
 LDCM0368  CL10 HCT 116 C353(1.04)  LDD0685  [1]
 LDCM0370  CL101 HCT 116 C353(1.85)  LDD0687  [1]
 LDCM0371  CL102 HCT 116 C353(1.48)  LDD0688  [1]
 LDCM0372  CL103 HCT 116 C353(1.20)  LDD0689  [1]
 LDCM0373  CL104 HCT 116 C353(1.32)  LDD0690  [1]
 LDCM0374  CL105 HCT 116 C353(0.81)  LDD0691  [1]
 LDCM0375  CL106 HCT 116 C353(0.77)  LDD0692  [1]
 LDCM0376  CL107 HCT 116 C353(0.82)  LDD0693  [1]
 LDCM0377  CL108 HCT 116 C353(0.99)  LDD0694  [1]
 LDCM0378  CL109 HCT 116 C353(1.81)  LDD0695  [1]
 LDCM0379  CL11 HCT 116 C353(1.59)  LDD0696  [1]
 LDCM0380  CL110 HCT 116 C353(0.96)  LDD0697  [1]
 LDCM0381  CL111 HCT 116 C353(1.02)  LDD0698  [1]
 LDCM0382  CL112 HCT 116 C353(1.53)  LDD0699  [1]
 LDCM0383  CL113 HCT 116 C353(1.59)  LDD0700  [1]
 LDCM0384  CL114 HCT 116 C353(1.33)  LDD0701  [1]
 LDCM0385  CL115 HCT 116 C353(1.76)  LDD0702  [1]
 LDCM0386  CL116 HCT 116 C353(1.81)  LDD0703  [1]
 LDCM0387  CL117 HCT 116 C353(1.71)  LDD0704  [1]
 LDCM0388  CL118 HCT 116 C353(1.26)  LDD0705  [1]
 LDCM0389  CL119 HCT 116 C353(1.28)  LDD0706  [1]
 LDCM0390  CL12 HCT 116 C353(1.22)  LDD0707  [1]
 LDCM0391  CL120 HCT 116 C353(1.07)  LDD0708  [1]
 LDCM0392  CL121 HCT 116 C353(0.81)  LDD0709  [1]
 LDCM0393  CL122 HCT 116 C353(0.96)  LDD0710  [1]
 LDCM0394  CL123 HCT 116 C353(2.39)  LDD0711  [1]
 LDCM0395  CL124 HCT 116 C353(1.65)  LDD0712  [1]
 LDCM0396  CL125 HCT 116 C353(0.92)  LDD0713  [1]
 LDCM0397  CL126 HCT 116 C353(0.70)  LDD0714  [1]
 LDCM0398  CL127 HCT 116 C353(0.75)  LDD0715  [1]
 LDCM0399  CL128 HCT 116 C353(0.67)  LDD0716  [1]
 LDCM0400  CL13 HCT 116 C353(1.26)  LDD0717  [1]
 LDCM0401  CL14 HCT 116 C353(1.74)  LDD0718  [1]
 LDCM0402  CL15 HCT 116 C353(2.36)  LDD0719  [1]
 LDCM0407  CL2 HCT 116 C353(1.52)  LDD0724  [1]
 LDCM0418  CL3 HCT 116 C353(2.38)  LDD0735  [1]
 LDCM0420  CL31 HCT 116 C353(0.65)  LDD0737  [1]
 LDCM0421  CL32 HCT 116 C353(0.97)  LDD0738  [1]
 LDCM0422  CL33 HCT 116 C353(0.99)  LDD0739  [1]
 LDCM0423  CL34 HCT 116 C353(1.25)  LDD0740  [1]
 LDCM0424  CL35 HCT 116 C353(1.20)  LDD0741  [1]
 LDCM0425  CL36 HCT 116 C353(1.35)  LDD0742  [1]
 LDCM0426  CL37 HCT 116 C353(1.14)  LDD0743  [1]
 LDCM0428  CL39 HCT 116 C353(1.07)  LDD0745  [1]
 LDCM0429  CL4 HCT 116 C353(2.45)  LDD0746  [1]
 LDCM0430  CL40 HCT 116 C353(1.19)  LDD0747  [1]
 LDCM0431  CL41 HCT 116 C353(1.06)  LDD0748  [1]
 LDCM0432  CL42 HCT 116 C353(1.27)  LDD0749  [1]
 LDCM0433  CL43 HCT 116 C353(1.13)  LDD0750  [1]
 LDCM0434  CL44 HCT 116 C353(1.13)  LDD0751  [1]
 LDCM0435  CL45 HCT 116 C353(1.36)  LDD0752  [1]
 LDCM0436  CL46 HCT 116 C353(0.95)  LDD0753  [1]
 LDCM0437  CL47 HCT 116 C353(0.84)  LDD0754  [1]
 LDCM0438  CL48 HCT 116 C353(1.03)  LDD0755  [1]
 LDCM0439  CL49 HCT 116 C353(1.10)  LDD0756  [1]
 LDCM0440  CL5 HCT 116 C353(1.17)  LDD0757  [1]
 LDCM0441  CL50 HCT 116 C353(1.05)  LDD0758  [1]
 LDCM0442  CL51 HCT 116 C353(1.02)  LDD0759  [1]
 LDCM0443  CL52 HCT 116 C353(0.87)  LDD0760  [1]
 LDCM0444  CL53 HCT 116 C353(1.01)  LDD0761  [1]
 LDCM0445  CL54 HCT 116 C353(1.03)  LDD0762  [1]
 LDCM0446  CL55 HCT 116 C353(0.85)  LDD0763  [1]
 LDCM0447  CL56 HCT 116 C353(0.83)  LDD0764  [1]
 LDCM0448  CL57 HCT 116 C353(0.88)  LDD0765  [1]
 LDCM0449  CL58 HCT 116 C353(0.92)  LDD0766  [1]
 LDCM0450  CL59 HCT 116 C353(1.09)  LDD0767  [1]
 LDCM0451  CL6 HCT 116 C353(2.12)  LDD0768  [1]
 LDCM0452  CL60 HCT 116 C353(1.08)  LDD0769  [1]
 LDCM0453  CL61 HCT 116 C353(1.30)  LDD0770  [1]
 LDCM0454  CL62 HCT 116 C353(1.68)  LDD0771  [1]
 LDCM0455  CL63 HCT 116 C353(2.26)  LDD0772  [1]
 LDCM0456  CL64 HCT 116 C353(1.71)  LDD0773  [1]
 LDCM0457  CL65 HCT 116 C353(1.23)  LDD0774  [1]
 LDCM0458  CL66 HCT 116 C353(1.25)  LDD0775  [1]
 LDCM0459  CL67 HCT 116 C353(1.59)  LDD0776  [1]
 LDCM0460  CL68 HCT 116 C353(1.60)  LDD0777  [1]
 LDCM0461  CL69 HCT 116 C353(1.37)  LDD0778  [1]
 LDCM0462  CL7 HCT 116 C353(2.15)  LDD0779  [1]
 LDCM0463  CL70 HCT 116 C353(1.13)  LDD0780  [1]
 LDCM0464  CL71 HCT 116 C353(1.11)  LDD0781  [1]
 LDCM0465  CL72 HCT 116 C353(1.00)  LDD0782  [1]
 LDCM0466  CL73 HCT 116 C353(1.24)  LDD0783  [1]
 LDCM0467  CL74 HCT 116 C353(1.68)  LDD0784  [1]
 LDCM0473  CL8 HCT 116 C353(4.08)  LDD0790  [1]
 LDCM0484  CL9 HCT 116 C353(2.05)  LDD0801  [1]
 LDCM0468  Fragment33 HCT 116 C353(1.97)  LDD0785  [1]
 LDCM0427  Fragment51 HCT 116 C353(1.03)  LDD0744  [1]
 LDCM0021  THZ1 HCT 116 C353(1.19)  LDD2173  [1]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 3 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Mitogen-activated protein kinase 1 (MAPK1) CMGC Ser/Thr protein kinase family P28482
Mitogen-activated protein kinase 3 (MAPK3) CMGC Ser/Thr protein kinase family P27361
Tyrosine-protein kinase TXK (TXK) Tyr protein kinase family P42681
Transporter and channel
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Amyloid-beta precursor protein (APP) APP family P05067
Other
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Prohibitin-2 (PHB2) Prohibitin family Q99623

References

1 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.