Details of the Target

General Information of Target

Target ID LDTP03319
Target Name 3-mercaptopyruvate sulfurtransferase (MPST)
Gene Name MPST
Gene ID 4357
Synonyms
TST2; 3-mercaptopyruvate sulfurtransferase; MST; EC 2.8.1.2
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MASPQLCRALVSAQWVAEALRAPRAGQPLQLLDASWYLPKLGRDARREFEERHIPGAAFF
DIDQCSDRTSPYDHMLPGAEHFAEYAGRLGVGAATHVVIYDASDQGLYSAPRVWWMFRAF
GHHAVSLLDGGLRHWLRQNLPLSSGKSQPAPAEFRAQLDPAFIKTYEDIKENLESRRFQV
VDSRATGRFRGTEPEPRDGIEPGHIPGTVNIPFTDFLSQEGLEKSPEEIRHLFQEKKVDL
SKPLVATCGSGVTACHVALGAYLCGKPDVPIYDGSWVEWYMRARPEDVISEGRGKTH
Target Bioclass
Enzyme
Subcellular location
Cytoplasm
Function
Transfer of a sulfur ion to cyanide or to other thiol compounds. Also has weak rhodanese activity. Detoxifies cyanide and is required for thiosulfate biosynthesis. Acts as an antioxidant. In combination with cysteine aminotransferase (CAT), contributes to the catabolism of cysteine and is an important producer of hydrogen sulfide in the brain, retina and vascular endothelial cells. Hydrogen sulfide H(2)S is an important synaptic modulator, signaling molecule, smooth muscle contractor and neuroprotectant. Its production by the 3MST/CAT pathway is regulated by calcium ions.
Uniprot ID
P25325
Ensemble ID
ENST00000341116.7
HGNC ID
HGNC:7223

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 25 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
m-APA
 Probe Info  		13.25  LDD0402  [1]
Jackson_1
 Probe Info  		20.00  LDD0122  [2]
N1
 Probe Info  		100.00  LDD0242  [3]
TH211
 Probe Info  		Y37(7.49)  LDD0260  [4]
C-Sul
 Probe Info  		2.60  LDD0066  [5]
STPyne
 Probe Info  		K164(1.11)  LDD0277  [6]
Jackson_14
 Probe Info  		3.03  LDD0123  [2]
DBIA
 Probe Info  		C65(1.66)  LDD0533  [7]
5E-2FA
 Probe Info  		H123(0.00); H122(0.00); H96(0.00); H231(0.00)  LDD2235  [8]
4-Iodoacetamidophenylacetylene
 Probe Info  		N.A.  LDD0038  [9]
IA-alkyne
 Probe Info  		N.A.  LDD0036  [9]
IPIAA_L
 Probe Info  		N.A.  LDD0031  [10]
Lodoacetamide azide
 Probe Info  		N.A.  LDD0037  [9]
IPM
 Probe Info  		N.A.  LDD0025  [11]
JW-RF-010
 Probe Info  		N.A.  LDD0026  [11]
TFBX
 Probe Info  		N.A.  LDD0027  [11]
ENE
 Probe Info  		N.A.  LDD0006  [12]
VSF
 Probe Info  		N.A.  LDD0007  [12]
Phosphinate-6
 Probe Info  		N.A.  LDD0018  [13]
1c-yne
 Probe Info  		K164(0.00); K224(0.00)  LDD0228  [14]
Acrolein
 Probe Info  		N.A.  LDD0217  [15]
W1
 Probe Info  		N.A.  LDD0236  [16]
NAIA_5
 Probe Info  		N.A.  LDD2223  [17]
HHS-465
 Probe Info  		N.A.  LDD2240  [18]
HHS-482
 Probe Info  		Y166(1.17)  LDD2239  [19]
PAL-AfBPP Probe
Click To Hide/Show 2 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C289
 Probe Info  		25.11  LDD1959  [20]
FFF probe2
 Probe Info  		5.46  LDD0463  [21]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0216  AC100 HCT 116 C65(1.66)  LDD0533  [7]
 LDCM0217  AC101 HCT 116 C65(0.73)  LDD0534  [7]
 LDCM0218  AC102 HCT 116 C65(0.84)  LDD0535  [7]
 LDCM0219  AC103 HCT 116 C65(0.41)  LDD0536  [7]
 LDCM0220  AC104 HCT 116 C65(0.55)  LDD0537  [7]
 LDCM0221  AC105 HCT 116 C65(0.49)  LDD0538  [7]
 LDCM0222  AC106 HCT 116 C65(0.49)  LDD0539  [7]
 LDCM0223  AC107 HCT 116 C65(0.59)  LDD0540  [7]
 LDCM0224  AC108 HCT 116 C65(0.50)  LDD0541  [7]
 LDCM0225  AC109 HCT 116 C65(0.54)  LDD0542  [7]
 LDCM0227  AC110 HCT 116 C65(0.52)  LDD0544  [7]
 LDCM0228  AC111 HCT 116 C65(0.42)  LDD0545  [7]
 LDCM0229  AC112 HCT 116 C65(0.52)  LDD0546  [7]
 LDCM0263  AC143 HCT 116 C65(0.81)  LDD0580  [7]
 LDCM0264  AC144 HCT 116 C65(0.45)  LDD0581  [7]
 LDCM0265  AC145 HCT 116 C65(0.76)  LDD0582  [7]
 LDCM0266  AC146 HCT 116 C65(0.70)  LDD0583  [7]
 LDCM0267  AC147 HCT 116 C65(0.37)  LDD0584  [7]
 LDCM0268  AC148 HCT 116 C65(0.13)  LDD0585  [7]
 LDCM0269  AC149 HCT 116 C65(0.39)  LDD0586  [7]
 LDCM0271  AC150 HCT 116 C65(0.78)  LDD0588  [7]
 LDCM0272  AC151 HCT 116 C65(0.88)  LDD0589  [7]
 LDCM0273  AC152 HCT 116 C65(0.33)  LDD0590  [7]
 LDCM0274  AC153 HCT 116 C65(0.11)  LDD0591  [7]
 LDCM0621  AC154 HCT 116 C65(0.50)  LDD2158  [7]
 LDCM0622  AC155 HCT 116 C65(0.57)  LDD2159  [7]
 LDCM0623  AC156 HCT 116 C65(0.76)  LDD2160  [7]
 LDCM0624  AC157 HCT 116 C65(0.91)  LDD2161  [7]
 LDCM0285  AC25 HCT 116 C65(1.21)  LDD0602  [7]
 LDCM0286  AC26 HCT 116 C65(0.95)  LDD0603  [7]
 LDCM0287  AC27 HCT 116 C65(1.10)  LDD0604  [7]
 LDCM0288  AC28 HCT 116 C65(0.86)  LDD0605  [7]
 LDCM0289  AC29 HCT 116 C65(1.18)  LDD0606  [7]
 LDCM0291  AC30 HCT 116 C65(1.25)  LDD0608  [7]
 LDCM0292  AC31 HCT 116 C65(1.15)  LDD0609  [7]
 LDCM0293  AC32 HCT 116 C65(0.79)  LDD0610  [7]
 LDCM0294  AC33 HCT 116 C65(0.92)  LDD0611  [7]
 LDCM0295  AC34 HCT 116 C65(0.69)  LDD0612  [7]
 LDCM0296  AC35 HCT 116 C65(1.50)  LDD0613  [7]
 LDCM0297  AC36 HCT 116 C65(1.16)  LDD0614  [7]
 LDCM0298  AC37 HCT 116 C65(1.32)  LDD0615  [7]
 LDCM0299  AC38 HCT 116 C65(1.50)  LDD0616  [7]
 LDCM0300  AC39 HCT 116 C65(0.95)  LDD0617  [7]
 LDCM0302  AC40 HCT 116 C65(0.55)  LDD0619  [7]
 LDCM0303  AC41 HCT 116 C65(1.07)  LDD0620  [7]
 LDCM0304  AC42 HCT 116 C65(0.89)  LDD0621  [7]
 LDCM0305  AC43 HCT 116 C65(1.09)  LDD0622  [7]
 LDCM0306  AC44 HCT 116 C65(0.75)  LDD0623  [7]
 LDCM0307  AC45 HCT 116 C65(0.53)  LDD0624  [7]
 LDCM0308  AC46 HCT 116 C65(1.30)  LDD0625  [7]
 LDCM0309  AC47 HCT 116 C65(0.94)  LDD0626  [7]
 LDCM0310  AC48 HCT 116 C65(1.91)  LDD0627  [7]
 LDCM0311  AC49 HCT 116 C65(0.30)  LDD0628  [7]
 LDCM0313  AC50 HCT 116 C65(0.41)  LDD0630  [7]
 LDCM0314  AC51 HCT 116 C65(2.06)  LDD0631  [7]
 LDCM0315  AC52 HCT 116 C65(0.94)  LDD0632  [7]
 LDCM0316  AC53 HCT 116 C65(0.62)  LDD0633  [7]
 LDCM0317  AC54 HCT 116 C65(0.52)  LDD0634  [7]
 LDCM0318  AC55 HCT 116 C65(0.33)  LDD0635  [7]
 LDCM0319  AC56 HCT 116 C65(0.20)  LDD0636  [7]
 LDCM0320  AC57 HCT 116 C65(1.16)  LDD0637  [7]
 LDCM0321  AC58 HCT 116 C65(1.03)  LDD0638  [7]
 LDCM0322  AC59 HCT 116 C65(0.79)  LDD0639  [7]
 LDCM0324  AC60 HCT 116 C65(1.66)  LDD0641  [7]
 LDCM0325  AC61 HCT 116 C65(1.71)  LDD0642  [7]
 LDCM0326  AC62 HCT 116 C65(0.76)  LDD0643  [7]
 LDCM0327  AC63 HCT 116 C65(1.27)  LDD0644  [7]
 LDCM0328  AC64 HCT 116 C65(0.74)  LDD0645  [7]
 LDCM0329  AC65 HCT 116 C65(0.93)  LDD0646  [7]
 LDCM0330  AC66 HCT 116 C65(1.60)  LDD0647  [7]
 LDCM0331  AC67 HCT 116 C65(0.32)  LDD0648  [7]
 LDCM0332  AC68 HCT 116 C65(0.75)  LDD0649  [7]
 LDCM0333  AC69 HCT 116 C65(0.59)  LDD0650  [7]
 LDCM0335  AC70 HCT 116 C65(0.28)  LDD0652  [7]
 LDCM0336  AC71 HCT 116 C65(1.20)  LDD0653  [7]
 LDCM0337  AC72 HCT 116 C65(0.54)  LDD0654  [7]
 LDCM0338  AC73 HCT 116 C65(0.17)  LDD0655  [7]
 LDCM0339  AC74 HCT 116 C65(0.21)  LDD0656  [7]
 LDCM0340  AC75 HCT 116 C65(0.13)  LDD0657  [7]
 LDCM0341  AC76 HCT 116 C65(0.36)  LDD0658  [7]
 LDCM0342  AC77 HCT 116 C65(0.34)  LDD0659  [7]
 LDCM0343  AC78 HCT 116 C65(1.36)  LDD0660  [7]
 LDCM0344  AC79 HCT 116 C65(0.80)  LDD0661  [7]
 LDCM0346  AC80 HCT 116 C65(0.47)  LDD0663  [7]
 LDCM0347  AC81 HCT 116 C65(1.10)  LDD0664  [7]
 LDCM0348  AC82 HCT 116 C65(0.12)  LDD0665  [7]
 LDCM0365  AC98 HCT 116 C65(0.09)  LDD0682  [7]
 LDCM0366  AC99 HCT 116 C65(0.77)  LDD0683  [7]
 LDCM0156  Aniline NCI-H1299 13.15  LDD0403  [1]
 LDCM0108  Chloroacetamide HeLa C65(0.00); H123(0.00)  LDD0222  [15]
 LDCM0632  CL-Sc Hep-G2 C65(20.00)  LDD2227  [17]
 LDCM0382  CL112 HCT 116 C65(1.07)  LDD0699  [7]
 LDCM0383  CL113 HCT 116 C65(0.84)  LDD0700  [7]
 LDCM0384  CL114 HCT 116 C65(0.94)  LDD0701  [7]
 LDCM0385  CL115 HCT 116 C65(0.97)  LDD0702  [7]
 LDCM0386  CL116 HCT 116 C65(1.04)  LDD0703  [7]
 LDCM0387  CL117 HCT 116 C65(0.25)  LDD0704  [7]
 LDCM0388  CL118 HCT 116 C65(0.78)  LDD0705  [7]
 LDCM0389  CL119 HCT 116 C65(0.78)  LDD0706  [7]
 LDCM0391  CL120 HCT 116 C65(1.04)  LDD0708  [7]
 LDCM0392  CL121 HCT 116 C65(0.74)  LDD0709  [7]
 LDCM0393  CL122 HCT 116 C65(1.04)  LDD0710  [7]
 LDCM0394  CL123 HCT 116 C65(0.37)  LDD0711  [7]
 LDCM0395  CL124 HCT 116 C65(0.39)  LDD0712  [7]
 LDCM0396  CL125 HCT 116 C65(2.25)  LDD0713  [7]
 LDCM0397  CL126 HCT 116 C65(0.88)  LDD0714  [7]
 LDCM0398  CL127 HCT 116 C65(0.95)  LDD0715  [7]
 LDCM0399  CL128 HCT 116 C65(0.70)  LDD0716  [7]
 LDCM0469  CL76 HCT 116 C65(0.88)  LDD0786  [7]
 LDCM0470  CL77 HCT 116 C65(0.58)  LDD0787  [7]
 LDCM0471  CL78 HCT 116 C65(0.72)  LDD0788  [7]
 LDCM0472  CL79 HCT 116 C65(0.38)  LDD0789  [7]
 LDCM0474  CL80 HCT 116 C65(1.42)  LDD0791  [7]
 LDCM0475  CL81 HCT 116 C65(0.67)  LDD0792  [7]
 LDCM0476  CL82 HCT 116 C65(0.24)  LDD0793  [7]
 LDCM0477  CL83 HCT 116 C65(0.31)  LDD0794  [7]
 LDCM0478  CL84 HCT 116 C65(0.19)  LDD0795  [7]
 LDCM0479  CL85 HCT 116 C65(0.89)  LDD0796  [7]
 LDCM0480  CL86 HCT 116 C65(0.87)  LDD0797  [7]
 LDCM0481  CL87 HCT 116 C65(0.49)  LDD0798  [7]
 LDCM0482  CL88 HCT 116 C65(0.33)  LDD0799  [7]
 LDCM0483  CL89 HCT 116 C65(0.17)  LDD0800  [7]
 LDCM0485  CL90 HCT 116 C65(0.64)  LDD0802  [7]
 LDCM0625  F8 Ramos C65(1.07)  LDD2187  [22]
 LDCM0572  Fragment10 Ramos C65(0.78)  LDD2189  [22]
 LDCM0573  Fragment11 Ramos C65(5.27)  LDD2190  [22]
 LDCM0574  Fragment12 Ramos C65(0.65)  LDD2191  [22]
 LDCM0575  Fragment13 Ramos C65(0.89)  LDD2192  [22]
 LDCM0576  Fragment14 Ramos C65(1.22)  LDD2193  [22]
 LDCM0579  Fragment20 Ramos C65(0.53)  LDD2194  [22]
 LDCM0580  Fragment21 Ramos C65(0.73)  LDD2195  [22]
 LDCM0582  Fragment23 Ramos C65(0.77)  LDD2196  [22]
 LDCM0578  Fragment27 Ramos C65(0.86)  LDD2197  [22]
 LDCM0586  Fragment28 Ramos C65(0.81)  LDD2198  [22]
 LDCM0588  Fragment30 Ramos C65(0.70)  LDD2199  [22]
 LDCM0589  Fragment31 Ramos C65(1.08)  LDD2200  [22]
 LDCM0590  Fragment32 Ramos C65(0.53)  LDD2201  [22]
 LDCM0468  Fragment33 Ramos C65(0.61)  LDD2202  [22]
 LDCM0596  Fragment38 Ramos C65(1.34)  LDD2203  [22]
 LDCM0566  Fragment4 Ramos C65(0.92)  LDD2184  [22]
 LDCM0610  Fragment52 Ramos C65(1.33)  LDD2204  [22]
 LDCM0614  Fragment56 Ramos C65(0.76)  LDD2205  [22]
 LDCM0569  Fragment7 Ramos C65(0.95)  LDD2186  [22]
 LDCM0571  Fragment9 Ramos C65(0.44)  LDD2188  [22]
 LDCM0107  IAA HeLa C65(0.00); H53(0.00); H231(0.00)  LDD0221  [15]
 LDCM0022  KB02 Ramos C65(0.74)  LDD2182  [22]
 LDCM0023  KB03 Ramos C65(0.75)  LDD2183  [22]
 LDCM0024  KB05 HMCB C85(1.63)  LDD3312  [23]
 LDCM0109  NEM HeLa N.A.  LDD0227  [15]
 LDCM0627  NUDT7-COV-1 HEK-293T C7(0.80)  LDD2206  [24]
 LDCM0628  OTUB2-COV-1 HEK-293T C7(0.95)  LDD2207  [24]
 LDCM0016  Ranjitkar_cp1 MDA-MB-231 3.03  LDD0123  [2]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
E3 SUMO-protein ligase ZBED1 (ZBED1) . O96006

References

1 Quantitative and Site-Specific Chemoproteomic Profiling of Targets of Acrolein. Chem Res Toxicol. 2019 Mar 18;32(3):467-473. doi: 10.1021/acs.chemrestox.8b00343. Epub 2019 Jan 15.
2 Appendage and Scaffold Diverse Fully Functionalized Small-Molecule Probes via a Minimalist Terminal Alkyne-Aliphatic Diazirine Isocyanide. J Org Chem. 2018 Sep 21;83(18):11245-11253. doi: 10.1021/acs.joc.8b01831. Epub 2018 Aug 31.
3 Cyclopropenone, Cyclopropeniminium Ion, and Cyclopropenethione as Novel Electrophilic Warheads for Potential Target Discovery of Triple-Negative Breast Cancer. J Med Chem. 2023 Feb 23;66(4):2851-2864. doi: 10.1021/acs.jmedchem.2c01889. Epub 2023 Feb 10.
4 Chemoproteomic profiling of kinases in live cells using electrophilic sulfonyl triazole probes. Chem Sci. 2021 Jan 21;12(9):3295-3307. doi: 10.1039/d0sc06623k.
5 Low-Toxicity Sulfonium-Based Probes for Cysteine-Specific Profiling in Live Cells. Anal Chem. 2022 Mar 15;94(10):4366-4372. doi: 10.1021/acs.analchem.1c05129. Epub 2022 Mar 4.
6 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
7 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
8 Global profiling of functional histidines in live cells using small-molecule photosensitizer and chemical probe relay labelling. Nat Chem. 2024 Jun 4. doi: 10.1038/s41557-024-01545-6. Online ahead of print.
Mass spectrometry data entry: PXD042377
9 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
10 SP3-Enabled Rapid and High Coverage Chemoproteomic Identification of Cell-State-Dependent Redox-Sensitive Cysteines. Mol Cell Proteomics. 2022 Apr;21(4):100218. doi: 10.1016/j.mcpro.2022.100218. Epub 2022 Feb 25.
Mass spectrometry data entry: PXD029500 , PXD031647
11 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
12 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
13 DFT-Guided Discovery of Ethynyl-Triazolyl-Phosphinates as Modular Electrophiles for Chemoselective Cysteine Bioconjugation and Profiling. Angew Chem Int Ed Engl. 2022 Oct 10;61(41):e202205348. doi: 10.1002/anie.202205348. Epub 2022 Aug 22.
Mass spectrometry data entry: PXD033004
14 Tunable Amine-Reactive Electrophiles for Selective Profiling of Lysine. Angew Chem Int Ed Engl. 2022 Jan 26;61(5):e202112107. doi: 10.1002/anie.202112107. Epub 2021 Dec 16.
15 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
16 Oxidant-Induced Bioconjugation for Protein Labeling in Live Cells. ACS Chem Biol. 2023 Jan 20;18(1):112-122. doi: 10.1021/acschembio.2c00740. Epub 2022 Dec 21.
17 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
18 Global profiling identifies a stress-responsive tyrosine site on EDC3 regulating biomolecular condensate formation. Cell Chem Biol. 2022 Dec 15;29(12):1709-1720.e7. doi: 10.1016/j.chembiol.2022.11.008. Epub 2022 Dec 6.
Mass spectrometry data entry: PXD038010
19 Global targeting of functional tyrosines using sulfur-triazole exchange chemistry. Nat Chem Biol. 2020 Feb;16(2):150-159. doi: 10.1038/s41589-019-0404-5. Epub 2019 Nov 25.
20 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587
21 Ligand and Target Discovery by Fragment-Based Screening in Human Cells. Cell. 2017 Jan 26;168(3):527-541.e29. doi: 10.1016/j.cell.2016.12.029. Epub 2017 Jan 19.
22 Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry. Methods Enzymol. 2023;679:295-322. doi: 10.1016/bs.mie.2022.07.037. Epub 2022 Sep 7.
Mass spectrometry data entry: PXD027578
23 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
24 Rapid Covalent-Probe Discovery by Electrophile-Fragment Screening. J Am Chem Soc. 2019 Jun 5;141(22):8951-8968. doi: 10.1021/jacs.9b02822. Epub 2019 May 22.