Details of the Target

General Information of Target

Target ID LDTP01616
Target Name CTD nuclear envelope phosphatase 1 (CTDNEP1)
Gene Name CTDNEP1
Gene ID 23399
Synonyms
DULLARD; CTD nuclear envelope phosphatase 1; EC 3.1.3.16; Serine/threonine-protein phosphatase dullard
3D Structure
Download
2D Sequence (FASTA)
Download
3D Structure (PDB)
Download
Sequence
MMRTQCLLGLRTFVAFAAKLWSFFIYLLRRQIRTVIQYQTVRYDILPLSPVSRNRLAQVK
RKILVLDLDETLIHSHHDGVLRPTVRPGTPPDFILKVVIDKHPVRFFVHKRPHVDFFLEV
VSQWYELVVFTASMEIYGSAVADKLDNSRSILKRRYYRQHCTLELGSYIKDLSVVHSDLS
SIVILDNSPGAYRSHPDNAIPIKSWFSDPSDTALLNLLPMLDALRFTADVRSVLSRNLHQ
HRLW
Target Bioclass
Enzyme
Family
Dullard family
Subcellular location
Endoplasmic reticulum membrane
Function
Serine/threonine protein phosphatase forming with CNEP1R1 an active phosphatase complex that dephosphorylates and may activate LPIN1 and LPIN2. LPIN1 and LPIN2 are phosphatidate phosphatases that catalyze the conversion of phosphatidic acid to diacylglycerol and control the metabolism of fatty acids at different levels. May indirectly modulate the lipid composition of nuclear and/or endoplasmic reticulum membranes and be required for proper nuclear membrane morphology and/or dynamics. May also indirectly regulate the production of lipid droplets and triacylglycerol. May antagonize BMP signaling.
Uniprot ID
O95476
Ensemble ID
ENST00000318988.10
HGNC ID
HGNC:19085

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 8 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
FBP2
 Probe Info  		3.14  LDD0317  [1]
IPM
 Probe Info  		N.A.  LDD0241  [2]
BTD
 Probe Info  		C6(2.43)  LDD2100  [3]
AHL-Pu-1
 Probe Info  		C6(3.69)  LDD0170  [4]
IA-alkyne
 Probe Info  		C6(4.85)  LDD2187  [5]
DBIA
 Probe Info  		C6(0.96)  LDD0580  [6]
TFBX
 Probe Info  		N.A.  LDD0027  [7]
YN-1
 Probe Info  		N.A.  LDD0446  [8]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0025  4SU-RNA DM93 C6(3.69)  LDD0170  [4]
 LDCM0026  4SU-RNA+native RNA DM93 C6(9.51)  LDD0171  [4]
 LDCM0215  AC10 HEK-293T C6(0.87)  LDD1508  [9]
 LDCM0226  AC11 HEK-293T C6(0.99)  LDD1509  [9]
 LDCM0263  AC143 HCT 116 C6(0.96)  LDD0580  [6]
 LDCM0264  AC144 HCT 116 C6(0.51)  LDD0581  [6]
 LDCM0265  AC145 HCT 116 C6(0.72)  LDD0582  [6]
 LDCM0266  AC146 HCT 116 C6(0.85)  LDD0583  [6]
 LDCM0267  AC147 HCT 116 C6(0.80)  LDD0584  [6]
 LDCM0268  AC148 HCT 116 C6(0.67)  LDD0585  [6]
 LDCM0269  AC149 HCT 116 C6(0.73)  LDD0586  [6]
 LDCM0271  AC150 HCT 116 C6(0.69)  LDD0588  [6]
 LDCM0272  AC151 HCT 116 C6(0.86)  LDD0589  [6]
 LDCM0273  AC152 HCT 116 C6(0.87)  LDD0590  [6]
 LDCM0274  AC153 HCT 116 C6(0.76)  LDD0591  [6]
 LDCM0621  AC154 HCT 116 C6(0.63)  LDD2158  [6]
 LDCM0622  AC155 HCT 116 C6(0.83)  LDD2159  [6]
 LDCM0623  AC156 HCT 116 C6(0.78)  LDD2160  [6]
 LDCM0624  AC157 HCT 116 C6(1.03)  LDD2161  [6]
 LDCM0276  AC17 HCT 116 C6(1.07)  LDD0593  [6]
 LDCM0277  AC18 HCT 116 C6(0.91)  LDD0594  [6]
 LDCM0278  AC19 HCT 116 C6(1.28)  LDD0595  [6]
 LDCM0279  AC2 HEK-293T C6(0.91)  LDD1518  [9]
 LDCM0280  AC20 HCT 116 C6(1.27)  LDD0597  [6]
 LDCM0281  AC21 HCT 116 C6(1.25)  LDD0598  [6]
 LDCM0282  AC22 HCT 116 C6(0.82)  LDD0599  [6]
 LDCM0283  AC23 HCT 116 C6(1.08)  LDD0600  [6]
 LDCM0284  AC24 HCT 116 C6(1.07)  LDD0601  [6]
 LDCM0285  AC25 HCT 116 C6(0.71)  LDD0602  [6]
 LDCM0286  AC26 HCT 116 C6(1.18)  LDD0603  [6]
 LDCM0287  AC27 HCT 116 C6(0.86)  LDD0604  [6]
 LDCM0288  AC28 HCT 116 C6(1.19)  LDD0605  [6]
 LDCM0289  AC29 HCT 116 C6(1.08)  LDD0606  [6]
 LDCM0290  AC3 HEK-293T C6(0.95)  LDD1529  [9]
 LDCM0291  AC30 HCT 116 C6(0.98)  LDD0608  [6]
 LDCM0292  AC31 HCT 116 C6(0.95)  LDD0609  [6]
 LDCM0293  AC32 HCT 116 C6(0.92)  LDD0610  [6]
 LDCM0294  AC33 HCT 116 C6(0.92)  LDD0611  [6]
 LDCM0295  AC34 HCT 116 C6(0.67)  LDD0612  [6]
 LDCM0296  AC35 HCT 116 C6(0.98)  LDD0613  [6]
 LDCM0297  AC36 HCT 116 C6(1.05)  LDD0614  [6]
 LDCM0298  AC37 HCT 116 C6(1.35)  LDD0615  [6]
 LDCM0299  AC38 HCT 116 C6(0.90)  LDD0616  [6]
 LDCM0300  AC39 HCT 116 C6(1.07)  LDD0617  [6]
 LDCM0302  AC40 HCT 116 C6(1.04)  LDD0619  [6]
 LDCM0303  AC41 HCT 116 C6(1.34)  LDD0620  [6]
 LDCM0304  AC42 HCT 116 C6(1.23)  LDD0621  [6]
 LDCM0305  AC43 HCT 116 C6(1.21)  LDD0622  [6]
 LDCM0306  AC44 HCT 116 C6(1.10)  LDD0623  [6]
 LDCM0307  AC45 HCT 116 C6(1.48)  LDD0624  [6]
 LDCM0310  AC48 HEK-293T C6(1.02)  LDD1549  [9]
 LDCM0313  AC50 HEK-293T C6(0.97)  LDD1552  [9]
 LDCM0314  AC51 HEK-293T C6(0.95)  LDD1553  [9]
 LDCM0319  AC56 HEK-293T C6(1.03)  LDD1558  [9]
 LDCM0321  AC58 HEK-293T C6(1.02)  LDD1560  [9]
 LDCM0322  AC59 HEK-293T C6(0.97)  LDD1561  [9]
 LDCM0328  AC64 HEK-293T C6(0.92)  LDD1567  [9]
 LDCM0345  AC8 HEK-293T C6(1.01)  LDD1569  [9]
 LDCM0275  AKOS034007705 HEK-293T C6(1.04)  LDD1514  [9]
 LDCM0367  CL1 HCT 116 C6(0.73)  LDD0684  [6]
 LDCM0368  CL10 HCT 116 C6(0.55)  LDD0685  [6]
 LDCM0370  CL101 HEK-293T C6(1.10)  LDD1574  [9]
 LDCM0374  CL105 HCT 116 C6(1.69)  LDD0691  [6]
 LDCM0375  CL106 HCT 116 C6(1.12)  LDD0692  [6]
 LDCM0376  CL107 HCT 116 C6(1.30)  LDD0693  [6]
 LDCM0377  CL108 HCT 116 C6(1.09)  LDD0694  [6]
 LDCM0378  CL109 HCT 116 C6(0.99)  LDD0695  [6]
 LDCM0379  CL11 HCT 116 C6(0.74)  LDD0696  [6]
 LDCM0380  CL110 HCT 116 C6(1.01)  LDD0697  [6]
 LDCM0381  CL111 HCT 116 C6(1.22)  LDD0698  [6]
 LDCM0382  CL112 HCT 116 C6(0.92)  LDD0699  [6]
 LDCM0383  CL113 HCT 116 C6(0.92)  LDD0700  [6]
 LDCM0384  CL114 HCT 116 C6(0.98)  LDD0701  [6]
 LDCM0385  CL115 HCT 116 C6(0.89)  LDD0702  [6]
 LDCM0386  CL116 HCT 116 C6(0.82)  LDD0703  [6]
 LDCM0387  CL117 HCT 116 C6(1.01)  LDD0704  [6]
 LDCM0388  CL118 HCT 116 C6(1.47)  LDD0705  [6]
 LDCM0389  CL119 HCT 116 C6(1.07)  LDD0706  [6]
 LDCM0390  CL12 HCT 116 C6(0.60)  LDD0707  [6]
 LDCM0391  CL120 HCT 116 C6(1.25)  LDD0708  [6]
 LDCM0392  CL121 HEK-293T C6(1.09)  LDD1596  [9]
 LDCM0396  CL125 HEK-293T C6(1.04)  LDD1600  [9]
 LDCM0400  CL13 HCT 116 C6(0.67)  LDD0717  [6]
 LDCM0401  CL14 HCT 116 C6(0.73)  LDD0718  [6]
 LDCM0402  CL15 HCT 116 C6(0.96)  LDD0719  [6]
 LDCM0405  CL18 HEK-293T C6(1.10)  LDD1609  [9]
 LDCM0406  CL19 HEK-293T C6(1.25)  LDD1610  [9]
 LDCM0407  CL2 HCT 116 C6(0.58)  LDD0724  [6]
 LDCM0412  CL24 HEK-293T C6(1.52)  LDD1616  [9]
 LDCM0413  CL25 HEK-293T C6(1.51)  LDD1617  [9]
 LDCM0418  CL3 HCT 116 C6(0.67)  LDD0735  [6]
 LDCM0419  CL30 HEK-293T C6(0.91)  LDD1623  [9]
 LDCM0420  CL31 HEK-293T C6(1.27)  LDD1624  [9]
 LDCM0425  CL36 HEK-293T C6(1.48)  LDD1629  [9]
 LDCM0426  CL37 HEK-293T C6(1.22)  LDD1630  [9]
 LDCM0429  CL4 HCT 116 C6(1.29)  LDD0746  [6]
 LDCM0432  CL42 HEK-293T C6(0.97)  LDD1636  [9]
 LDCM0433  CL43 HEK-293T C6(1.05)  LDD1637  [9]
 LDCM0438  CL48 HEK-293T C6(1.21)  LDD1642  [9]
 LDCM0439  CL49 HEK-293T C6(1.20)  LDD1643  [9]
 LDCM0440  CL5 HCT 116 C6(0.56)  LDD0757  [6]
 LDCM0445  CL54 HEK-293T C6(1.55)  LDD1648  [9]
 LDCM0446  CL55 HEK-293T C6(1.16)  LDD1649  [9]
 LDCM0451  CL6 HCT 116 C6(0.77)  LDD0768  [6]
 LDCM0452  CL60 HEK-293T C6(1.29)  LDD1655  [9]
 LDCM0453  CL61 HCT 116 C6(1.31)  LDD0770  [6]
 LDCM0454  CL62 HCT 116 C6(1.11)  LDD0771  [6]
 LDCM0455  CL63 HCT 116 C6(1.34)  LDD0772  [6]
 LDCM0456  CL64 HCT 116 C6(1.19)  LDD0773  [6]
 LDCM0457  CL65 HCT 116 C6(1.06)  LDD0774  [6]
 LDCM0458  CL66 HCT 116 C6(1.09)  LDD0775  [6]
 LDCM0459  CL67 HCT 116 C6(1.02)  LDD0776  [6]
 LDCM0460  CL68 HCT 116 C6(0.96)  LDD0777  [6]
 LDCM0461  CL69 HCT 116 C6(1.39)  LDD0778  [6]
 LDCM0462  CL7 HCT 116 C6(0.82)  LDD0779  [6]
 LDCM0463  CL70 HCT 116 C6(0.97)  LDD0780  [6]
 LDCM0464  CL71 HCT 116 C6(1.00)  LDD0781  [6]
 LDCM0465  CL72 HCT 116 C6(1.05)  LDD0782  [6]
 LDCM0466  CL73 HCT 116 C6(1.25)  LDD0783  [6]
 LDCM0467  CL74 HCT 116 C6(1.14)  LDD0784  [6]
 LDCM0469  CL76 HCT 116 C6(0.92)  LDD0786  [6]
 LDCM0470  CL77 HCT 116 C6(0.79)  LDD0787  [6]
 LDCM0471  CL78 HCT 116 C6(0.85)  LDD0788  [6]
 LDCM0472  CL79 HCT 116 C6(0.77)  LDD0789  [6]
 LDCM0473  CL8 HCT 116 C6(0.68)  LDD0790  [6]
 LDCM0474  CL80 HCT 116 C6(0.89)  LDD0791  [6]
 LDCM0475  CL81 HCT 116 C6(0.86)  LDD0792  [6]
 LDCM0476  CL82 HCT 116 C6(0.84)  LDD0793  [6]
 LDCM0477  CL83 HCT 116 C6(0.98)  LDD0794  [6]
 LDCM0478  CL84 HCT 116 C6(0.87)  LDD0795  [6]
 LDCM0479  CL85 HCT 116 C6(0.91)  LDD0796  [6]
 LDCM0480  CL86 HCT 116 C6(0.92)  LDD0797  [6]
 LDCM0481  CL87 HCT 116 C6(0.71)  LDD0798  [6]
 LDCM0482  CL88 HCT 116 C6(0.73)  LDD0799  [6]
 LDCM0483  CL89 HCT 116 C6(0.98)  LDD0800  [6]
 LDCM0484  CL9 HCT 116 C6(0.69)  LDD0801  [6]
 LDCM0485  CL90 HCT 116 C6(0.87)  LDD0802  [6]
 LDCM0486  CL91 HEK-293T C6(1.08)  LDD1689  [9]
 LDCM0491  CL96 HEK-293T C6(1.42)  LDD1694  [9]
 LDCM0492  CL97 HEK-293T C6(1.53)  LDD1695  [9]
 LDCM0213  Electrophilic fragment 2 MDA-MB-231 C6(8.64)  LDD1702  [3]
 LDCM0625  F8 Ramos C6(4.85)  LDD2187  [5]
 LDCM0573  Fragment11 Ramos C6(0.56)  LDD2190  [5]
 LDCM0575  Fragment13 Ramos C6(3.87)  LDD2192  [5]
 LDCM0582  Fragment23 Ramos C6(1.81)  LDD2196  [5]
 LDCM0578  Fragment27 Ramos C6(1.07)  LDD2197  [5]
 LDCM0586  Fragment28 Ramos C6(1.47)  LDD2198  [5]
 LDCM0468  Fragment33 HCT 116 C6(0.93)  LDD0785  [6]
 LDCM0614  Fragment56 Ramos C6(1.49)  LDD2205  [5]
 LDCM0022  KB02 GB-2 C6(1.62)  LDD2335  [10]
 LDCM0023  KB03 DM93 C6(1.89)  LDD2889  [10]
 LDCM0024  KB05 SKMEL24 C6(1.60)  LDD3323  [10]
 LDCM0507  Nucleophilic fragment 16b MDA-MB-231 C6(2.43)  LDD2100  [3]
 LDCM0512  Nucleophilic fragment 19a MDA-MB-231 C6(1.23)  LDD2105  [3]
 LDCM0544  Nucleophilic fragment 39 MDA-MB-231 C6(0.64)  LDD2137  [3]
 LDCM0546  Nucleophilic fragment 40 MDA-MB-231 C6(0.61)  LDD2140  [3]
 LDCM0547  Nucleophilic fragment 41 MDA-MB-231 C6(0.90)  LDD2141  [3]
 LDCM0551  Nucleophilic fragment 5b MDA-MB-231 C6(5.47)  LDD2145  [3]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Other
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Nuclear envelope phosphatase-regulatory subunit 1 (CNEP1R1) CNEP1R1 family Q8N9A8
Ubiquilin-2 (UBQLN2) . Q9UHD9

References

1 Tranylcypromine specificity for monoamine oxidase is limited by promiscuous protein labelling and lysosomal trapping. RSC Chem Biol. 2020 Aug 12;1(4):209-213. doi: 10.1039/d0cb00048e. eCollection 2020 Oct 1.
Mass spectrometry data entry: PXD018580
2 Oxidant-Induced Bioconjugation for Protein Labeling in Live Cells. ACS Chem Biol. 2023 Jan 20;18(1):112-122. doi: 10.1021/acschembio.2c00740. Epub 2022 Dec 21.
3 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
4 Chemoproteomic capture of RNA binding activity in living cells. Nat Commun. 2023 Oct 7;14(1):6282. doi: 10.1038/s41467-023-41844-z.
Mass spectrometry data entry: PXD044625
5 Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry. Methods Enzymol. 2023;679:295-322. doi: 10.1016/bs.mie.2022.07.037. Epub 2022 Sep 7.
Mass spectrometry data entry: PXD027578
6 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
7 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
8 Ynamide Electrophile for the Profiling of Ligandable Carboxyl Residues in Live Cells and the Development of New Covalent Inhibitors. J Med Chem. 2022 Aug 11;65(15):10408-10418. doi: 10.1021/acs.jmedchem.2c00272. Epub 2022 Jul 26.
9 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
10 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840