Details of the Target

General Information of Target

Target ID LDTP01129
Target Name CLIP-associating protein 2 (CLASP2)
Gene Name CLASP2
Gene ID 23122
Synonyms
KIAA0627; CLIP-associating protein 2; Cytoplasmic linker-associated protein 2; Protein Orbit homolog 2; hOrbit2
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MAMGDDKSFDDEESVDGNRPSSAASAFKVPAPKTSGNPANSARKPGSAGGPKVGGASKEG
GAGAVDEDDFIKAFTDVPSIQIYSSRELEETLNKIREILSDDKHDWDQRANALKKIRSLL
VAGAAQYDCFFQHLRLLDGALKLSAKDLRSQVVREACITVAHLSTVLGNKFDHGAEAIVP
TLFNLVPNSAKVMATSGCAAIRFIIRHTHVPRLIPLITSNCTSKSVPVRRRSFEFLDLLL
QEWQTHSLERHAAVLVETIKKGIHDADAEARVEARKTYMGLRNHFPGEAETLYNSLEPSY
QKSLQTYLKSSGSVASLPQSDRSSSSSQESLNRPFSSKWSTANPSTVAGRVSAGSSKASS
LPGSLQRSRSDIDVNAAAGAKAHHAAGQSVRSGRLGAGALNAGSYASLEDTSDKLDGTAS
EDGRVRAKLSAPLAGMGNAKADSRGRSRTKMVSQSQPGSRSGSPGRVLTTTALSTVSSGV
QRVLVNSASAQKRSKIPRSQGCSREASPSRLSVARSSRIPRPSVSQGCSREASRESSRDT
SPVRSFQPLASRHHSRSTGALYAPEVYGASGPGYGISQSSRLSSSVSAMRVLNTGSDVEE
AVADALKKPARRRYESYGMHSDDDANSDASSACSERSYSSRNGSIPTYMRQTEDVAEVLN
RCASSNWSERKEGLLGLQNLLKNQRTLSRVELKRLCEIFTRMFADPHGKRVFSMFLETLV
DFIQVHKDDLQDWLFVLLTQLLKKMGADLLGSVQAKVQKALDVTRESFPNDLQFNILMRF
TVDQTQTPSLKVKVAILKYIETLAKQMDPGDFINSSETRLAVSRVITWTTEPKSSDVRKA
AQSVLISLFELNTPEFTMLLGALPKTFQDGATKLLHNHLRNTGNGTQSSMGSPLTRPTPR
SPANWSSPLTSPTNTSQNTLSPSAFDYDTENMNSEDIYSSLRGVTEAIQNFSFRSQEDMN
EPLKRDSKKDDGDSMCGGPGMSDPRAGGDATDSSQTALDNKASLLHSMPTHSSPRSRDYN
PYNYSDSISPFNKSALKEAMFDDDADQFPDDLSLDHSDLVAELLKELSNHNERVEERKIA
LYELMKLTQEESFSVWDEHFKTILLLLLETLGDKEPTIRALALKVLREILRHQPARFKNY
AELTVMKTLEAHKDPHKEVVRSAEEAASVLATSISPEQCIKVLCPIIQTADYPINLAAIK
MQTKVIERVSKETLNLLLPEIMPGLIQGYDNSESSVRKACVFCLVAVHAVIGDELKPHLS
QLTGSKMKLLNLYIKRAQTGSGGADPTTDVSGQS
Target Bioclass
Other
Family
CLASP family
Subcellular location
Cytoplasm, cytoskeleton
Function
Microtubule plus-end tracking protein that promotes the stabilization of dynamic microtubules. Involved in the nucleation of noncentrosomal microtubules originating from the trans-Golgi network (TGN). Required for the polarization of the cytoplasmic microtubule arrays in migrating cells towards the leading edge of the cell. May act at the cell cortex to enhance the frequency of rescue of depolymerizing microtubules by attaching their plus-ends to cortical platforms composed of ERC1 and PHLDB2. This cortical microtubule stabilizing activity is regulated at least in part by phosphatidylinositol 3-kinase signaling. Also performs a similar stabilizing function at the kinetochore which is essential for the bipolar alignment of chromosomes on the mitotic spindle. Acts as a mediator of ERBB2-dependent stabilization of microtubules at the cell cortex.
Uniprot ID
O75122
Ensemble ID
ENST00000313350.10
HGNC ID
HGNC:17078

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 21 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
m-APA
 Probe Info  		15.00  LDD0402  [1]
STPyne
 Probe Info  		K682(9.09)  LDD0277  [2]
BTD
 Probe Info  		C198(1.32)  LDD1700  [3]
AHL-Pu-1
 Probe Info  		C976(2.73); C633(3.18)  LDD0168  [4]
EA-probe
 Probe Info  		N.A.  LDD0440  [5]
DBIA
 Probe Info  		C633(24.61)  LDD0204  [6]
HHS-475
 Probe Info  		Y1273(0.78)  LDD0264  [7]
HHS-465
 Probe Info  		Y1273(10.00)  LDD2237  [8]
ATP probe
 Probe Info  		K671(0.00); K1033(0.00); K1037(0.00); K450(0.00)  LDD0199  [9]
IA-alkyne
 Probe Info  		C696(0.00); C662(0.00); C198(0.00); C221(0.00)  LDD0162  [10]
NAIA_4
 Probe Info  		C633(0.00); C662(0.00)  LDD2226  [11]
TFBX
 Probe Info  		C1184(0.00); C198(0.00); C1179(0.00)  LDD0027  [12]
WYneN
 Probe Info  		C1184(0.00); C633(0.00); C976(0.00)  LDD0021  [13]
DA-P3
 Probe Info  		N.A.  LDD0178  [14]
IPM
 Probe Info  		C528(0.00); C976(0.00)  LDD0005  [13]
VSF
 Probe Info  		N.A.  LDD0007  [13]
Phosphinate-6
 Probe Info  		C528(0.00); C976(0.00)  LDD0018  [15]
Acrolein
 Probe Info  		C1184(0.00); C221(0.00)  LDD0217  [16]
W1
 Probe Info  		C1184(0.00); C221(0.00); C528(0.00)  LDD0236  [17]
AOyne
 Probe Info  		8.90  LDD0443  [18]
NAIA_5
 Probe Info  		C662(0.00); C221(0.00); C1179(0.00); C129(0.00)  LDD2223  [11]
PAL-AfBPP Probe
Click To Hide/Show 1 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C407
 Probe Info  		11.88  LDD2064  [19]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0524  2-Cyano-N-(2-morpholin-4-yl-ethyl)-acetamide MDA-MB-231 C528(0.70)  LDD2117  [3]
 LDCM0558  2-Cyano-N-phenylacetamide MDA-MB-231 C198(1.25); C528(1.22); C502(1.22)  LDD2152  [3]
 LDCM0025  4SU-RNA HEK-293T C976(2.73); C633(3.18)  LDD0168  [4]
 LDCM0026  4SU-RNA+native RNA HEK-293T C976(2.60); C633(2.66)  LDD0169  [4]
 LDCM0156  Aniline NCI-H1299 11.35  LDD0403  [1]
 LDCM0102  BDHI 8 Jurkat C633(24.61)  LDD0204  [6]
 LDCM0108  Chloroacetamide HeLa C1184(0.00); C221(0.00)  LDD0222  [16]
 LDCM0632  CL-Sc Hep-G2 C633(0.84); C221(0.82)  LDD2227  [11]
 LDCM0213  Electrophilic fragment 2 MDA-MB-231 C221(2.27); C976(1.19)  LDD1702  [3]
 LDCM0031  Epigallocatechin gallate HEK-293T 6.59  LDD0183  [14]
 LDCM0175  Ethacrynic acid HeLa N.A.  LDD0440  [5]
 LDCM0625  F8 Ramos C198(0.96); C662(1.68); C528(1.04); C976(1.09)  LDD2187  [20]
 LDCM0572  Fragment10 Ramos C198(1.32); C662(0.95); C528(1.13); C976(1.38)  LDD2189  [20]
 LDCM0573  Fragment11 Ramos C198(4.63); C633(8.97)  LDD2190  [20]
 LDCM0574  Fragment12 Ramos C198(1.89); C662(0.73); C528(0.85); C976(0.42)  LDD2191  [20]
 LDCM0575  Fragment13 Ramos C198(1.39); C662(0.83); C528(0.60); C976(1.51)  LDD2192  [20]
 LDCM0576  Fragment14 Ramos C198(1.38); C662(1.09); C528(1.00); C633(1.58)  LDD2193  [20]
 LDCM0579  Fragment20 Ramos C198(1.60); C662(0.76); C528(0.83); C976(0.35)  LDD2194  [20]
 LDCM0580  Fragment21 Ramos C198(1.10); C662(0.85); C528(0.62); C976(1.36)  LDD2195  [20]
 LDCM0582  Fragment23 Ramos C198(0.60); C662(0.84); C528(1.24); C976(1.41)  LDD2196  [20]
 LDCM0578  Fragment27 Ramos C198(1.09); C662(0.89); C528(0.76); C976(1.43)  LDD2197  [20]
 LDCM0586  Fragment28 Ramos C198(1.06); C662(0.77); C528(0.89); C976(0.94)  LDD2198  [20]
 LDCM0588  Fragment30 Ramos C198(1.57); C662(1.17); C528(0.98); C976(0.74)  LDD2199  [20]
 LDCM0589  Fragment31 Ramos C198(1.31); C662(0.95); C528(0.88)  LDD2200  [20]
 LDCM0590  Fragment32 Ramos C198(1.02); C662(0.83); C528(1.37); C976(0.31)  LDD2201  [20]
 LDCM0468  Fragment33 Ramos C198(1.09); C662(0.75); C528(0.87); C976(1.22)  LDD2202  [20]
 LDCM0596  Fragment38 Ramos C198(1.29); C662(1.19); C528(0.64); C976(0.73)  LDD2203  [20]
 LDCM0566  Fragment4 Ramos C198(1.59); C662(0.98); C528(1.05); C976(0.78)  LDD2184  [20]
 LDCM0610  Fragment52 Ramos C198(1.57); C528(1.16); C633(0.62)  LDD2204  [20]
 LDCM0614  Fragment56 Ramos C198(1.32); C662(1.11); C528(1.41); C976(0.77)  LDD2205  [20]
 LDCM0569  Fragment7 Ramos C198(1.68); C662(0.82); C528(1.08); C976(1.61)  LDD2186  [20]
 LDCM0571  Fragment9 Ramos C198(1.79); C662(0.99); C528(1.22); C976(1.08)  LDD2188  [20]
 LDCM0116  HHS-0101 DM93 Y1273(0.78)  LDD0264  [7]
 LDCM0117  HHS-0201 DM93 Y1273(0.82)  LDD0265  [7]
 LDCM0118  HHS-0301 DM93 Y1273(0.80)  LDD0266  [7]
 LDCM0119  HHS-0401 DM93 Y1273(0.79)  LDD0267  [7]
 LDCM0120  HHS-0701 DM93 Y1273(0.80)  LDD0268  [7]
 LDCM0107  IAA HeLa N.A.  LDD0221  [16]
 LDCM0022  KB02 Ramos C198(2.03); C662(0.74); C528(1.58); C976(2.56)  LDD2182  [20]
 LDCM0023  KB03 MDA-MB-231 C221(11.26)  LDD1701  [3]
 LDCM0024  KB05 G361 C883(2.57); C762(2.36); C455(2.17); C1428(2.23)  LDD3311  [21]
 LDCM0030  Luteolin HEK-293T 4.97  LDD0182  [14]
 LDCM0109  NEM HeLa N.A.  LDD0224  [16]
 LDCM0499  Nucleophilic fragment 12b MDA-MB-231 C696(0.92)  LDD2092  [3]
 LDCM0500  Nucleophilic fragment 13a MDA-MB-231 C198(1.07); C696(1.46)  LDD2093  [3]
 LDCM0501  Nucleophilic fragment 13b MDA-MB-231 C696(0.58)  LDD2094  [3]
 LDCM0506  Nucleophilic fragment 16a MDA-MB-231 C198(0.72); C696(1.56)  LDD2099  [3]
 LDCM0514  Nucleophilic fragment 20a MDA-MB-231 C198(1.09); C696(0.79)  LDD2107  [3]
 LDCM0516  Nucleophilic fragment 21a MDA-MB-231 C696(0.61)  LDD2109  [3]
 LDCM0518  Nucleophilic fragment 22a MDA-MB-231 C696(1.30)  LDD2111  [3]
 LDCM0525  Nucleophilic fragment 25b MDA-MB-231 C528(0.89)  LDD2118  [3]
 LDCM0526  Nucleophilic fragment 26a MDA-MB-231 C198(1.83); C696(1.45)  LDD2119  [3]
 LDCM0529  Nucleophilic fragment 27b MDA-MB-231 C528(0.77)  LDD2122  [3]
 LDCM0530  Nucleophilic fragment 28a MDA-MB-231 C198(0.91)  LDD2123  [3]
 LDCM0531  Nucleophilic fragment 28b MDA-MB-231 C528(0.89)  LDD2124  [3]
 LDCM0532  Nucleophilic fragment 29a MDA-MB-231 C198(0.65)  LDD2125  [3]
 LDCM0534  Nucleophilic fragment 30a MDA-MB-231 C198(0.95); C221(0.58)  LDD2127  [3]
 LDCM0536  Nucleophilic fragment 31 MDA-MB-231 C198(1.28)  LDD2129  [3]
 LDCM0542  Nucleophilic fragment 37 MDA-MB-231 C696(1.67)  LDD2135  [3]
 LDCM0543  Nucleophilic fragment 38 MDA-MB-231 C198(1.01)  LDD2136  [3]
 LDCM0544  Nucleophilic fragment 39 MDA-MB-231 C198(0.79); C696(0.91)  LDD2137  [3]
 LDCM0211  Nucleophilic fragment 3b MDA-MB-231 C198(1.32)  LDD1700  [3]
 LDCM0546  Nucleophilic fragment 40 MDA-MB-231 C198(0.73); C696(1.15)  LDD2140  [3]
 LDCM0549  Nucleophilic fragment 43 MDA-MB-231 C528(0.92)  LDD2143  [3]
 LDCM0550  Nucleophilic fragment 5a MDA-MB-231 C696(1.15)  LDD2144  [3]
 LDCM0552  Nucleophilic fragment 6a MDA-MB-231 C696(0.97)  LDD2146  [3]
 LDCM0553  Nucleophilic fragment 6b MDA-MB-231 C528(0.64)  LDD2147  [3]
 LDCM0554  Nucleophilic fragment 7a MDA-MB-231 C528(0.50)  LDD2148  [3]
 LDCM0032  Oleacein HEK-293T 5.66  LDD0184  [14]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Other
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Microtubule-associated protein RP/EB family member 1 (MAPRE1) MAPRE family Q15691

References

1 Quantitative and Site-Specific Chemoproteomic Profiling of Targets of Acrolein. Chem Res Toxicol. 2019 Mar 18;32(3):467-473. doi: 10.1021/acs.chemrestox.8b00343. Epub 2019 Jan 15.
2 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
3 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
4 Chemoproteomic capture of RNA binding activity in living cells. Nat Commun. 2023 Oct 7;14(1):6282. doi: 10.1038/s41467-023-41844-z.
Mass spectrometry data entry: PXD044625
5 Chemoproteomic Profiling Reveals Ethacrynic Acid Targets Adenine Nucleotide Translocases to Impair Mitochondrial Function. Mol Pharm. 2018 Jun 4;15(6):2413-2422. doi: 10.1021/acs.molpharmaceut.8b00250. Epub 2018 May 15.
6 Covalent Inhibition by a Natural Product-Inspired Latent Electrophile. J Am Chem Soc. 2023 May 24;145(20):11097-11109. doi: 10.1021/jacs.3c00598. Epub 2023 May 15.
7 Discovery of a Cell-Active SuTEx Ligand of Prostaglandin Reductase 2. Chembiochem. 2021 Jun 15;22(12):2134-2139. doi: 10.1002/cbic.202000879. Epub 2021 Apr 29.
8 Global targeting of functional tyrosines using sulfur-triazole exchange chemistry. Nat Chem Biol. 2020 Feb;16(2):150-159. doi: 10.1038/s41589-019-0404-5. Epub 2019 Nov 25.
9 Targeted Proteomic Approaches for Proteome-Wide Characterizations of the AMP-Binding Capacities of Kinases. J Proteome Res. 2022 Aug 5;21(8):2063-2070. doi: 10.1021/acs.jproteome.2c00225. Epub 2022 Jul 12.
10 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
11 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
12 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
13 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
14 A chemical probe unravels the reactive proteome of health-associated catechols. Chem Sci. 2023 Jul 22;14(32):8635-8643. doi: 10.1039/d3sc00888f. eCollection 2023 Aug 16.
Mass spectrometry data entry: PXD043348
15 DFT-Guided Discovery of Ethynyl-Triazolyl-Phosphinates as Modular Electrophiles for Chemoselective Cysteine Bioconjugation and Profiling. Angew Chem Int Ed Engl. 2022 Oct 10;61(41):e202205348. doi: 10.1002/anie.202205348. Epub 2022 Aug 22.
Mass spectrometry data entry: PXD033004
16 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
17 Oxidant-Induced Bioconjugation for Protein Labeling in Live Cells. ACS Chem Biol. 2023 Jan 20;18(1):112-122. doi: 10.1021/acschembio.2c00740. Epub 2022 Dec 21.
18 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
19 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587
20 Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry. Methods Enzymol. 2023;679:295-322. doi: 10.1016/bs.mie.2022.07.037. Epub 2022 Sep 7.
Mass spectrometry data entry: PXD027578
21 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840