Details of the Target

General Information of Target

Target ID LDTP00726
Target Name Beta-1,4-galactosyltransferase 5 (B4GALT5)
Gene Name B4GALT5
Gene ID 9334
Synonyms
Beta-1,4-galactosyltransferase 5; Beta-1,4-GalTase 5; Beta4Gal-T5; b4Gal-T5; EC 2.4.1.-; Beta-1,4-GalT II; Glucosylceramide beta-1,4-galactosyltransferase; EC 2.4.1.274; Lactosylceramide synthase; LacCer synthase; UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 5; UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 5
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MRARRGLLRLPRRSLLAALFFFSLSSSLLYFVYVAPGIVNTYLFMMQAQGILIRDNVRTI
GAQVYEQVLRSAYAKRNSSVNDSDYPLDLNHSETFLQTTTFLPEDFTYFANHTCPERLPS
MKGPIDINMSEIGMDYIHELFSKDPTIKLGGHWKPSDCMPRWKVAILIPFRNRHEHLPVL
FRHLLPMLQRQRLQFAFYVVEQVGTQPFNRAMLFNVGFQEAMKDLDWDCLIFHDVDHIPE
SDRNYYGCGQMPRHFATKLDKYMYLLPYTEFFGGVSGLTVEQFRKINGFPNAFWGWGGED
DDLWNRVQNAGYSVSRPEGDTGKYKSIPHHHRGEVQFLGRYALLRKSKERQGLDGLNNLN
YFANITYDALYKNITVNLTPELAQVNEY
Target Bioclass
Enzyme
Family
Glycosyltransferase 7 family
Subcellular location
Golgi apparatus, Golgi stack membrane
Function
Catalyzes the synthesis of lactosylceramide (LacCer) via the transfer of galactose from UDP-galactose to glucosylceramide (GlcCer). LacCer is the starting point in the biosynthesis of all gangliosides (membrane-bound glycosphingolipids) which play pivotal roles in the CNS including neuronal maturation and axonal and myelin formation. Plays a role in the glycosylation of BMPR1A and regulation of its protein stability. Essential for extraembryonic development during early embryogenesis.
Uniprot ID
O43286
Ensemble ID
ENST00000371711.4
HGNC ID
HGNC:928

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 1 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C248(1.27)  LDD0531  [1]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0214  AC1 HCT 116 C248(1.27)  LDD0531  [1]
 LDCM0216  AC100 HCT 116 C248(0.46)  LDD0533  [1]
 LDCM0217  AC101 HCT 116 C248(0.54)  LDD0534  [1]
 LDCM0218  AC102 HCT 116 C248(0.33)  LDD0535  [1]
 LDCM0219  AC103 HCT 116 C248(0.30)  LDD0536  [1]
 LDCM0220  AC104 HCT 116 C248(0.35)  LDD0537  [1]
 LDCM0221  AC105 HCT 116 C248(0.41)  LDD0538  [1]
 LDCM0222  AC106 HCT 116 C248(0.42)  LDD0539  [1]
 LDCM0223  AC107 HCT 116 C248(0.51)  LDD0540  [1]
 LDCM0224  AC108 HCT 116 C248(0.35)  LDD0541  [1]
 LDCM0225  AC109 HCT 116 C248(0.33)  LDD0542  [1]
 LDCM0227  AC110 HCT 116 C248(0.41)  LDD0544  [1]
 LDCM0228  AC111 HCT 116 C248(0.33)  LDD0545  [1]
 LDCM0229  AC112 HCT 116 C248(0.34)  LDD0546  [1]
 LDCM0246  AC128 HCT 116 C248(0.59)  LDD0563  [1]
 LDCM0247  AC129 HCT 116 C248(1.47)  LDD0564  [1]
 LDCM0249  AC130 HCT 116 C248(1.15)  LDD0566  [1]
 LDCM0250  AC131 HCT 116 C248(1.21)  LDD0567  [1]
 LDCM0251  AC132 HCT 116 C248(0.96)  LDD0568  [1]
 LDCM0252  AC133 HCT 116 C248(1.40)  LDD0569  [1]
 LDCM0253  AC134 HCT 116 C248(1.16)  LDD0570  [1]
 LDCM0254  AC135 HCT 116 C248(0.81)  LDD0571  [1]
 LDCM0255  AC136 HCT 116 C248(0.75)  LDD0572  [1]
 LDCM0256  AC137 HCT 116 C248(1.41)  LDD0573  [1]
 LDCM0257  AC138 HCT 116 C248(1.17)  LDD0574  [1]
 LDCM0258  AC139 HCT 116 C248(0.97)  LDD0575  [1]
 LDCM0260  AC140 HCT 116 C248(1.11)  LDD0577  [1]
 LDCM0261  AC141 HCT 116 C248(1.27)  LDD0578  [1]
 LDCM0262  AC142 HCT 116 C248(1.06)  LDD0579  [1]
 LDCM0263  AC143 HCT 116 C248(1.12)  LDD0580  [1]
 LDCM0264  AC144 HCT 116 C248(0.99)  LDD0581  [1]
 LDCM0265  AC145 HCT 116 C248(1.31)  LDD0582  [1]
 LDCM0266  AC146 HCT 116 C248(0.99)  LDD0583  [1]
 LDCM0267  AC147 HCT 116 C248(0.89)  LDD0584  [1]
 LDCM0268  AC148 HCT 116 C248(0.48)  LDD0585  [1]
 LDCM0269  AC149 HCT 116 C248(0.95)  LDD0586  [1]
 LDCM0271  AC150 HCT 116 C248(1.41)  LDD0588  [1]
 LDCM0272  AC151 HCT 116 C248(1.11)  LDD0589  [1]
 LDCM0273  AC152 HCT 116 C248(0.77)  LDD0590  [1]
 LDCM0274  AC153 HCT 116 C248(0.52)  LDD0591  [1]
 LDCM0621  AC154 HCT 116 C248(1.06)  LDD2158  [1]
 LDCM0622  AC155 HCT 116 C248(1.06)  LDD2159  [1]
 LDCM0623  AC156 HCT 116 C248(0.82)  LDD2160  [1]
 LDCM0624  AC157 HCT 116 C248(1.42)  LDD2161  [1]
 LDCM0276  AC17 HCT 116 C248(0.55)  LDD0593  [1]
 LDCM0277  AC18 HCT 116 C248(0.54)  LDD0594  [1]
 LDCM0278  AC19 HCT 116 C248(0.96)  LDD0595  [1]
 LDCM0279  AC2 HCT 116 C248(1.29)  LDD0596  [1]
 LDCM0280  AC20 HCT 116 C248(1.29)  LDD0597  [1]
 LDCM0281  AC21 HCT 116 C248(0.82)  LDD0598  [1]
 LDCM0282  AC22 HCT 116 C248(0.73)  LDD0599  [1]
 LDCM0283  AC23 HCT 116 C248(0.80)  LDD0600  [1]
 LDCM0284  AC24 HCT 116 C248(0.82)  LDD0601  [1]
 LDCM0285  AC25 HCT 116 C248(1.25)  LDD0602  [1]
 LDCM0286  AC26 HCT 116 C248(1.15)  LDD0603  [1]
 LDCM0287  AC27 HCT 116 C248(1.01)  LDD0604  [1]
 LDCM0288  AC28 HCT 116 C248(0.93)  LDD0605  [1]
 LDCM0289  AC29 HCT 116 C248(1.21)  LDD0606  [1]
 LDCM0290  AC3 HCT 116 C248(1.80)  LDD0607  [1]
 LDCM0291  AC30 HCT 116 C248(1.34)  LDD0608  [1]
 LDCM0292  AC31 HCT 116 C248(1.05)  LDD0609  [1]
 LDCM0293  AC32 HCT 116 C248(1.20)  LDD0610  [1]
 LDCM0294  AC33 HCT 116 C248(1.16)  LDD0611  [1]
 LDCM0295  AC34 HCT 116 C248(0.73)  LDD0612  [1]
 LDCM0301  AC4 HCT 116 C248(1.80)  LDD0618  [1]
 LDCM0302  AC40 HEK-293T C229(0.64)  LDD1541  [2]
 LDCM0303  AC41 HEK-293T C229(0.96)  LDD1542  [2]
 LDCM0308  AC46 HCT 116 C248(1.01)  LDD0625  [1]
 LDCM0309  AC47 HCT 116 C248(1.14)  LDD0626  [1]
 LDCM0310  AC48 HCT 116 C248(0.99)  LDD0627  [1]
 LDCM0311  AC49 HCT 116 C248(0.84)  LDD0628  [1]
 LDCM0312  AC5 HCT 116 C248(1.82)  LDD0629  [1]
 LDCM0313  AC50 HCT 116 C248(0.86)  LDD0630  [1]
 LDCM0314  AC51 HCT 116 C248(1.27)  LDD0631  [1]
 LDCM0315  AC52 HCT 116 C248(1.30)  LDD0632  [1]
 LDCM0316  AC53 HCT 116 C248(1.10)  LDD0633  [1]
 LDCM0317  AC54 HCT 116 C248(0.80)  LDD0634  [1]
 LDCM0318  AC55 HCT 116 C248(0.80)  LDD0635  [1]
 LDCM0319  AC56 HCT 116 C248(0.91)  LDD0636  [1]
 LDCM0320  AC57 HCT 116 C248(0.54)  LDD0637  [1]
 LDCM0321  AC58 HCT 116 C248(0.43)  LDD0638  [1]
 LDCM0322  AC59 HCT 116 C248(0.67)  LDD0639  [1]
 LDCM0324  AC60 HCT 116 C248(1.03)  LDD0641  [1]
 LDCM0325  AC61 HCT 116 C248(0.63)  LDD0642  [1]
 LDCM0326  AC62 HCT 116 C248(0.70)  LDD0643  [1]
 LDCM0327  AC63 HCT 116 C248(0.63)  LDD0644  [1]
 LDCM0328  AC64 HCT 116 C248(0.47)  LDD0645  [1]
 LDCM0329  AC65 HCT 116 C248(1.05)  LDD0646  [1]
 LDCM0330  AC66 HCT 116 C248(1.11)  LDD0647  [1]
 LDCM0331  AC67 HCT 116 C248(0.47)  LDD0648  [1]
 LDCM0332  AC68 HCT 116 C248(0.75)  LDD0649  [1]
 LDCM0333  AC69 HCT 116 C248(0.88)  LDD0650  [1]
 LDCM0335  AC70 HCT 116 C248(0.88)  LDD0652  [1]
 LDCM0336  AC71 HCT 116 C248(0.91)  LDD0653  [1]
 LDCM0337  AC72 HCT 116 C248(0.83)  LDD0654  [1]
 LDCM0338  AC73 HCT 116 C248(0.99)  LDD0655  [1]
 LDCM0339  AC74 HCT 116 C248(1.08)  LDD0656  [1]
 LDCM0340  AC75 HCT 116 C248(0.93)  LDD0657  [1]
 LDCM0341  AC76 HCT 116 C248(0.92)  LDD0658  [1]
 LDCM0342  AC77 HCT 116 C248(0.79)  LDD0659  [1]
 LDCM0343  AC78 HCT 116 C248(1.07)  LDD0660  [1]
 LDCM0344  AC79 HCT 116 C248(0.75)  LDD0661  [1]
 LDCM0345  AC8 HEK-293T C229(0.92)  LDD1569  [2]
 LDCM0346  AC80 HCT 116 C248(1.02)  LDD0663  [1]
 LDCM0347  AC81 HCT 116 C248(0.96)  LDD0664  [1]
 LDCM0348  AC82 HCT 116 C248(0.86)  LDD0665  [1]
 LDCM0365  AC98 HCT 116 C248(0.17)  LDD0682  [1]
 LDCM0366  AC99 HCT 116 C248(0.38)  LDD0683  [1]
 LDCM0356  AKOS034007680 HEK-293T C229(0.85)  LDD1570  [2]
 LDCM0275  AKOS034007705 HEK-293T C229(1.06)  LDD1514  [2]
 LDCM0367  CL1 HCT 116 C248(1.84)  LDD0684  [1]
 LDCM0368  CL10 HCT 116 C248(0.66)  LDD0685  [1]
 LDCM0369  CL100 HCT 116 C248(1.29)  LDD0686  [1]
 LDCM0374  CL105 HCT 116 C248(1.00)  LDD0691  [1]
 LDCM0375  CL106 HCT 116 C248(0.44)  LDD0692  [1]
 LDCM0376  CL107 HCT 116 C248(1.02)  LDD0693  [1]
 LDCM0377  CL108 HCT 116 C248(0.52)  LDD0694  [1]
 LDCM0378  CL109 HCT 116 C248(0.82)  LDD0695  [1]
 LDCM0379  CL11 HCT 116 C248(0.74)  LDD0696  [1]
 LDCM0380  CL110 HCT 116 C248(0.68)  LDD0697  [1]
 LDCM0381  CL111 HCT 116 C248(0.76)  LDD0698  [1]
 LDCM0382  CL112 HCT 116 C248(1.27)  LDD0699  [1]
 LDCM0383  CL113 HCT 116 C248(0.91)  LDD0700  [1]
 LDCM0384  CL114 HCT 116 C248(1.35)  LDD0701  [1]
 LDCM0385  CL115 HCT 116 C248(1.19)  LDD0702  [1]
 LDCM0386  CL116 HCT 116 C248(1.53)  LDD0703  [1]
 LDCM0390  CL12 HCT 116 C248(0.74)  LDD0707  [1]
 LDCM0392  CL121 HCT 116 C248(0.83)  LDD0709  [1]
 LDCM0393  CL122 HCT 116 C248(1.31)  LDD0710  [1]
 LDCM0394  CL123 HCT 116 C248(0.84)  LDD0711  [1]
 LDCM0395  CL124 HCT 116 C248(0.69)  LDD0712  [1]
 LDCM0396  CL125 HCT 116 C248(0.83)  LDD0713  [1]
 LDCM0397  CL126 HCT 116 C248(0.75)  LDD0714  [1]
 LDCM0398  CL127 HCT 116 C248(1.01)  LDD0715  [1]
 LDCM0399  CL128 HCT 116 C248(1.01)  LDD0716  [1]
 LDCM0400  CL13 HCT 116 C248(0.84)  LDD0717  [1]
 LDCM0401  CL14 HCT 116 C248(1.28)  LDD0718  [1]
 LDCM0402  CL15 HCT 116 C248(0.89)  LDD0719  [1]
 LDCM0404  CL17 HEK-293T C229(0.97)  LDD1608  [2]
 LDCM0407  CL2 HCT 116 C248(1.16)  LDD0724  [1]
 LDCM0412  CL24 HEK-293T C229(1.10)  LDD1616  [2]
 LDCM0417  CL29 HEK-293T C229(0.61)  LDD1621  [2]
 LDCM0418  CL3 HCT 116 C248(1.57)  LDD0735  [1]
 LDCM0420  CL31 HCT 116 C248(0.68)  LDD0737  [1]
 LDCM0421  CL32 HCT 116 C248(0.60)  LDD0738  [1]
 LDCM0422  CL33 HCT 116 C248(0.69)  LDD0739  [1]
 LDCM0423  CL34 HCT 116 C248(0.42)  LDD0740  [1]
 LDCM0424  CL35 HCT 116 C248(0.30)  LDD0741  [1]
 LDCM0425  CL36 HCT 116 C248(0.64)  LDD0742  [1]
 LDCM0426  CL37 HCT 116 C248(0.38)  LDD0743  [1]
 LDCM0428  CL39 HCT 116 C248(0.41)  LDD0745  [1]
 LDCM0429  CL4 HCT 116 C248(1.08)  LDD0746  [1]
 LDCM0430  CL40 HCT 116 C248(0.46)  LDD0747  [1]
 LDCM0431  CL41 HCT 116 C248(0.61)  LDD0748  [1]
 LDCM0432  CL42 HCT 116 C248(0.37)  LDD0749  [1]
 LDCM0433  CL43 HCT 116 C248(0.40)  LDD0750  [1]
 LDCM0434  CL44 HCT 116 C248(0.41)  LDD0751  [1]
 LDCM0435  CL45 HCT 116 C248(0.43)  LDD0752  [1]
 LDCM0438  CL48 HEK-293T C229(0.89)  LDD1642  [2]
 LDCM0440  CL5 HCT 116 C248(0.97)  LDD0757  [1]
 LDCM0444  CL53 HEK-293T C229(0.72)  LDD1647  [2]
 LDCM0451  CL6 HCT 116 C248(0.88)  LDD0768  [1]
 LDCM0452  CL60 HEK-293T C229(1.38)  LDD1655  [2]
 LDCM0453  CL61 HCT 116 C248(1.14)  LDD0770  [1]
 LDCM0454  CL62 HCT 116 C248(1.03)  LDD0771  [1]
 LDCM0455  CL63 HCT 116 C248(0.97)  LDD0772  [1]
 LDCM0456  CL64 HCT 116 C248(1.21)  LDD0773  [1]
 LDCM0457  CL65 HCT 116 C248(1.23)  LDD0774  [1]
 LDCM0458  CL66 HCT 116 C248(1.05)  LDD0775  [1]
 LDCM0459  CL67 HCT 116 C248(0.77)  LDD0776  [1]
 LDCM0460  CL68 HCT 116 C248(0.89)  LDD0777  [1]
 LDCM0461  CL69 HCT 116 C248(1.03)  LDD0778  [1]
 LDCM0462  CL7 HCT 116 C248(0.87)  LDD0779  [1]
 LDCM0463  CL70 HCT 116 C248(0.91)  LDD0780  [1]
 LDCM0464  CL71 HCT 116 C248(0.87)  LDD0781  [1]
 LDCM0465  CL72 HCT 116 C248(1.00)  LDD0782  [1]
 LDCM0466  CL73 HCT 116 C248(0.90)  LDD0783  [1]
 LDCM0467  CL74 HCT 116 C248(1.01)  LDD0784  [1]
 LDCM0469  CL76 HCT 116 C248(0.54)  LDD0786  [1]
 LDCM0470  CL77 HCT 116 C248(1.02)  LDD0787  [1]
 LDCM0471  CL78 HCT 116 C248(0.49)  LDD0788  [1]
 LDCM0472  CL79 HCT 116 C248(0.60)  LDD0789  [1]
 LDCM0473  CL8 HCT 116 C248(0.70)  LDD0790  [1]
 LDCM0474  CL80 HCT 116 C248(1.87)  LDD0791  [1]
 LDCM0475  CL81 HCT 116 C248(1.18)  LDD0792  [1]
 LDCM0476  CL82 HCT 116 C248(0.38)  LDD0793  [1]
 LDCM0477  CL83 HCT 116 C248(0.34)  LDD0794  [1]
 LDCM0478  CL84 HCT 116 C248(0.40)  LDD0795  [1]
 LDCM0479  CL85 HCT 116 C248(0.88)  LDD0796  [1]
 LDCM0480  CL86 HCT 116 C248(0.66)  LDD0797  [1]
 LDCM0481  CL87 HCT 116 C248(0.83)  LDD0798  [1]
 LDCM0482  CL88 HCT 116 C248(0.47)  LDD0799  [1]
 LDCM0483  CL89 HCT 116 C248(0.30)  LDD0800  [1]
 LDCM0484  CL9 HCT 116 C248(1.65)  LDD0801  [1]
 LDCM0485  CL90 HCT 116 C248(0.58)  LDD0802  [1]
 LDCM0486  CL91 HCT 116 C248(1.11)  LDD0803  [1]
 LDCM0487  CL92 HCT 116 C248(0.99)  LDD0804  [1]
 LDCM0488  CL93 HCT 116 C248(1.05)  LDD0805  [1]
 LDCM0489  CL94 HCT 116 C248(0.89)  LDD0806  [1]
 LDCM0490  CL95 HCT 116 C248(1.22)  LDD0807  [1]
 LDCM0491  CL96 HCT 116 C248(1.08)  LDD0808  [1]
 LDCM0492  CL97 HCT 116 C248(0.96)  LDD0809  [1]
 LDCM0493  CL98 HCT 116 C248(1.43)  LDD0810  [1]
 LDCM0494  CL99 HCT 116 C248(1.68)  LDD0811  [1]
 LDCM0468  Fragment33 HCT 116 C248(0.94)  LDD0785  [1]
 LDCM0427  Fragment51 HCT 116 C248(0.42)  LDD0744  [1]

References

1 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
2 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402