Details of the Target

General Information of Target

Target ID LDTP00519
Target Name NEDD4-binding protein 3 (N4BP3)
Gene Name N4BP3
Gene ID 23138
Synonyms
KIAA0341; NEDD4-binding protein 3; N4BP3
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MATAPGPAGIAMGSVGSLLERQDFSPEELRAALAGSRGSRQPDGLLRKGLGQREFLSYLH
LPKKDSKSTKNTKRAPRNEPADYATLYYREHSRAGDFSKTSLPERGRFDKCRIRPSVFKP
TAGNGKGFLSMQSLASHKGQKLWRSNGSLHTLACHPPLSPGPRASQARAQLLHALSLDEG
GPEPEPSLSDSSSGGSFGRSPGTGPSPFSSSLGHLNHLGGSLDRASQGPKEAGPPAVLSC
LPEPPPPYEFSCSSAEEMGAVLPETCEELKRGLGDEDGSNPFTQVLEERQRLWLAELKRL
YVERLHEVTQKAERSERNLQLQLFMAQQEQRRLRKELRAQQGLAPEPRAPGTLPEADPSA
RPEEEARWEVCQKTAEISLLKQQLREAQAELAQKLAEIFSLKTQLRGSRAQAQAQDAELV
RLREAVRSLQEQAPREEAPGSCETDDCKSRGLLGEAGGSEARDSAEQLRAELLQERLRGQ
EQALRFEQERRTWQEEKERVLRYQREIQGGYMDMYRRNQALEQELRALREPPTPWSPRLE
SSKI
Target Bioclass
Other
Family
N4BP3 family
Subcellular location
Cytoplasmic vesicle
Function
Plays a positive role in the antiviral innate immune signaling pathway. Mechanistically, interacts with MAVS and functions as a positive regulator to promote 'Lys-63'-linked polyubiquitination of MAVS and thus strengthens the interaction between MAVS and TRAF2. Also plays a role in axon and dendrite arborization during cranial nerve development. May also be important for neural crest migration and early development of other anterior structures including eye, brain and cranial cartilage.
Uniprot ID
O15049
Ensemble ID
ENST00000274605.6
HGNC ID
HGNC:29852

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 3 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
Curcusone 37
 Probe Info  		2.24  LDD0188  [1]
DBIA
 Probe Info  		C442(0.69); C447(0.69)  LDD0563  [2]
5E-2FA
 Probe Info  		N.A.  LDD2235  [3]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0246  AC128 HCT 116 C442(0.69); C447(0.69)  LDD0563  [2]
 LDCM0247  AC129 HCT 116 C442(0.68); C447(0.68)  LDD0564  [2]
 LDCM0249  AC130 HCT 116 C442(0.83); C447(0.83)  LDD0566  [2]
 LDCM0250  AC131 HCT 116 C442(0.90); C447(0.90)  LDD0567  [2]
 LDCM0251  AC132 HCT 116 C442(1.06); C447(1.06)  LDD0568  [2]
 LDCM0252  AC133 HCT 116 C442(0.96); C447(0.96)  LDD0569  [2]
 LDCM0253  AC134 HCT 116 C442(1.03); C447(1.03)  LDD0570  [2]
 LDCM0254  AC135 HCT 116 C442(0.83); C447(0.83)  LDD0571  [2]
 LDCM0255  AC136 HCT 116 C442(0.96); C447(0.96)  LDD0572  [2]
 LDCM0256  AC137 HCT 116 C442(0.83); C447(0.83)  LDD0573  [2]
 LDCM0257  AC138 HCT 116 C442(0.85); C447(0.85)  LDD0574  [2]
 LDCM0258  AC139 HCT 116 C442(1.04); C447(1.04)  LDD0575  [2]
 LDCM0260  AC140 HCT 116 C442(1.09); C447(1.09)  LDD0577  [2]
 LDCM0261  AC141 HCT 116 C442(1.22); C447(1.22)  LDD0578  [2]
 LDCM0262  AC142 HCT 116 C442(0.90); C447(0.90)  LDD0579  [2]
 LDCM0263  AC143 HCT 116 C442(0.71); C447(0.71)  LDD0580  [2]
 LDCM0264  AC144 HCT 116 C442(0.75); C447(0.75)  LDD0581  [2]
 LDCM0265  AC145 HCT 116 C442(0.69); C447(0.69)  LDD0582  [2]
 LDCM0266  AC146 HCT 116 C442(0.87); C447(0.87)  LDD0583  [2]
 LDCM0267  AC147 HCT 116 C442(0.85); C447(0.85)  LDD0584  [2]
 LDCM0268  AC148 HCT 116 C442(0.76); C447(0.76)  LDD0585  [2]
 LDCM0269  AC149 HCT 116 C442(0.98); C447(0.98)  LDD0586  [2]
 LDCM0271  AC150 HCT 116 C442(0.67); C447(0.67)  LDD0588  [2]
 LDCM0272  AC151 HCT 116 C442(1.23); C447(1.23)  LDD0589  [2]
 LDCM0273  AC152 HCT 116 C442(0.90); C447(0.90)  LDD0590  [2]
 LDCM0274  AC153 HCT 116 C442(0.83); C447(0.83)  LDD0591  [2]
 LDCM0621  AC154 HCT 116 C442(0.77); C447(0.77)  LDD2158  [2]
 LDCM0622  AC155 HCT 116 C442(0.73); C447(0.73)  LDD2159  [2]
 LDCM0623  AC156 HCT 116 C442(0.77); C447(0.77)  LDD2160  [2]
 LDCM0624  AC157 HCT 116 C442(0.82); C447(0.82)  LDD2161  [2]
 LDCM0403  CL16 HCT 116 C442(1.18); C447(1.18)  LDD0720  [2]
 LDCM0404  CL17 HCT 116 C442(0.86); C447(0.86)  LDD0721  [2]
 LDCM0405  CL18 HCT 116 C442(1.07); C447(1.07)  LDD0722  [2]
 LDCM0406  CL19 HCT 116 C442(0.90); C447(0.90)  LDD0723  [2]
 LDCM0408  CL20 HCT 116 C442(1.07); C447(1.07)  LDD0725  [2]
 LDCM0409  CL21 HCT 116 C442(0.92); C447(0.92)  LDD0726  [2]
 LDCM0410  CL22 HCT 116 C442(0.74); C447(0.74)  LDD0727  [2]
 LDCM0411  CL23 HCT 116 C442(1.28); C447(1.28)  LDD0728  [2]
 LDCM0412  CL24 HCT 116 C442(1.21); C447(1.21)  LDD0729  [2]
 LDCM0413  CL25 HCT 116 C442(1.19); C447(1.19)  LDD0730  [2]
 LDCM0414  CL26 HCT 116 C442(1.12); C447(1.12)  LDD0731  [2]
 LDCM0415  CL27 HCT 116 C442(1.22); C447(1.22)  LDD0732  [2]
 LDCM0416  CL28 HCT 116 C442(1.10); C447(1.10)  LDD0733  [2]
 LDCM0417  CL29 HCT 116 C442(1.27); C447(1.27)  LDD0734  [2]
 LDCM0419  CL30 HCT 116 C442(1.27); C447(1.27)  LDD0736  [2]
 LDCM0421  CL32 HCT 116 C442(1.32); C447(1.32)  LDD0738  [2]
 LDCM0422  CL33 HCT 116 C442(1.16); C447(1.16)  LDD0739  [2]
 LDCM0423  CL34 HCT 116 C442(1.08); C447(1.08)  LDD0740  [2]
 LDCM0424  CL35 HCT 116 C442(1.10); C447(1.10)  LDD0741  [2]
 LDCM0425  CL36 HCT 116 C442(0.88); C447(0.88)  LDD0742  [2]
 LDCM0426  CL37 HCT 116 C442(1.14); C447(1.14)  LDD0743  [2]
 LDCM0428  CL39 HCT 116 C442(1.14); C447(1.14)  LDD0745  [2]
 LDCM0430  CL40 HCT 116 C442(1.75); C447(1.75)  LDD0747  [2]
 LDCM0431  CL41 HCT 116 C442(0.88); C447(0.88)  LDD0748  [2]
 LDCM0432  CL42 HCT 116 C442(0.87); C447(0.87)  LDD0749  [2]
 LDCM0433  CL43 HCT 116 C442(0.99); C447(0.99)  LDD0750  [2]
 LDCM0434  CL44 HCT 116 C442(0.94); C447(0.94)  LDD0751  [2]
 LDCM0435  CL45 HCT 116 C442(1.08); C447(1.08)  LDD0752  [2]
 LDCM0436  CL46 HCT 116 C442(0.97); C447(0.97)  LDD0753  [2]
 LDCM0437  CL47 HCT 116 C442(0.92); C447(0.92)  LDD0754  [2]
 LDCM0438  CL48 HCT 116 C442(0.79); C447(0.79)  LDD0755  [2]
 LDCM0439  CL49 HCT 116 C442(0.95); C447(0.95)  LDD0756  [2]
 LDCM0441  CL50 HCT 116 C442(0.99); C447(0.99)  LDD0758  [2]
 LDCM0442  CL51 HCT 116 C442(0.99); C447(0.99)  LDD0759  [2]
 LDCM0443  CL52 HCT 116 C442(1.28); C447(1.28)  LDD0760  [2]
 LDCM0444  CL53 HCT 116 C442(1.05); C447(1.05)  LDD0761  [2]
 LDCM0445  CL54 HCT 116 C442(1.14); C447(1.14)  LDD0762  [2]
 LDCM0446  CL55 HCT 116 C442(0.98); C447(0.98)  LDD0763  [2]
 LDCM0447  CL56 HCT 116 C442(0.79); C447(0.79)  LDD0764  [2]
 LDCM0448  CL57 HCT 116 C442(0.87); C447(0.87)  LDD0765  [2]
 LDCM0449  CL58 HCT 116 C442(1.23); C447(1.23)  LDD0766  [2]
 LDCM0450  CL59 HCT 116 C442(1.17); C447(1.17)  LDD0767  [2]
 LDCM0452  CL60 HCT 116 C442(0.96); C447(0.96)  LDD0769  [2]
 LDCM0453  CL61 HCT 116 C442(1.07); C447(1.07)  LDD0770  [2]
 LDCM0454  CL62 HCT 116 C442(1.88); C447(1.88)  LDD0771  [2]
 LDCM0455  CL63 HCT 116 C442(1.02); C447(1.02)  LDD0772  [2]
 LDCM0456  CL64 HCT 116 C442(0.82); C447(0.82)  LDD0773  [2]
 LDCM0457  CL65 HCT 116 C442(1.76); C447(1.76)  LDD0774  [2]
 LDCM0458  CL66 HCT 116 C442(1.19); C447(1.19)  LDD0775  [2]
 LDCM0459  CL67 HCT 116 C442(1.12); C447(1.12)  LDD0776  [2]
 LDCM0460  CL68 HCT 116 C442(1.22); C447(1.22)  LDD0777  [2]
 LDCM0461  CL69 HCT 116 C442(1.34); C447(1.34)  LDD0778  [2]
 LDCM0463  CL70 HCT 116 C442(1.13); C447(1.13)  LDD0780  [2]
 LDCM0464  CL71 HCT 116 C442(1.20); C447(1.20)  LDD0781  [2]
 LDCM0465  CL72 HCT 116 C442(1.21); C447(1.21)  LDD0782  [2]
 LDCM0466  CL73 HCT 116 C442(1.33); C447(1.33)  LDD0783  [2]
 LDCM0467  CL74 HCT 116 C442(1.12); C447(1.12)  LDD0784  [2]
 LDCM0469  CL76 HCT 116 C442(1.14); C447(1.14)  LDD0786  [2]
 LDCM0470  CL77 HCT 116 C442(0.87); C447(0.87)  LDD0787  [2]
 LDCM0471  CL78 HCT 116 C442(1.01); C447(1.01)  LDD0788  [2]
 LDCM0472  CL79 HCT 116 C442(1.10); C447(1.10)  LDD0789  [2]
 LDCM0474  CL80 HCT 116 C442(0.92); C447(0.92)  LDD0791  [2]
 LDCM0475  CL81 HCT 116 C442(1.09); C447(1.09)  LDD0792  [2]
 LDCM0476  CL82 HCT 116 C442(0.88); C447(0.88)  LDD0793  [2]
 LDCM0477  CL83 HCT 116 C442(1.01); C447(1.01)  LDD0794  [2]
 LDCM0478  CL84 HCT 116 C442(1.12); C447(1.12)  LDD0795  [2]
 LDCM0479  CL85 HCT 116 C442(0.98); C447(0.98)  LDD0796  [2]
 LDCM0480  CL86 HCT 116 C442(1.06); C447(1.06)  LDD0797  [2]
 LDCM0481  CL87 HCT 116 C442(1.17); C447(1.17)  LDD0798  [2]
 LDCM0482  CL88 HCT 116 C442(1.10); C447(1.10)  LDD0799  [2]
 LDCM0483  CL89 HCT 116 C442(1.16); C447(1.16)  LDD0800  [2]
 LDCM0485  CL90 HCT 116 C442(1.01); C447(1.01)  LDD0802  [2]
 LDCM0033  Curcusone1d MCF-7 2.24  LDD0188  [1]
 LDCM0468  Fragment33 HCT 116 C442(1.01); C447(1.01)  LDD0785  [2]
 LDCM0427  Fragment51 HCT 116 C442(0.91); C447(0.91)  LDD0744  [2]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 5 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Maspardin (SPG21) AB hydrolase superfamily Q9NZD8
Replication factor C subunit 5 (RFC5) Activator 1 small subunits family P40937
Protein N-terminal glutamine amidohydrolase (NTAQ1) NTAQ1 family Q96HA8
Carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 2 (CTDSP2) . O14595
E3 ubiquitin-protein ligase NRDP1 (RNF41) . Q9H4P4
Transporter and channel
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Syntenin-1 (SDCBP) . O00560
Other
Click To Hide/Show 6 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Connector enhancer of kinase suppressor of ras 3 (CNKSR3) CNKSR family Q6P9H4
AP-4 complex accessory subunit RUSC1 (RUSC1) . Q9BVN2
Coiled-coil domain-containing protein 146 (CCDC146) . Q8IYE0
Enkurin domain-containing protein 1 (ENKD1) . Q9H0I2
LIM domain transcription factor LMO4 (LMO4) . P61968
Proline-rich protein 35 (PRR35) . P0CG20

References

1 Total Synthesis and Target Identification of the Curcusone Diterpenes. J Am Chem Soc. 2021 Mar 24;143(11):4379-4386. doi: 10.1021/jacs.1c00557. Epub 2021 Mar 11.
2 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
3 Global profiling of functional histidines in live cells using small-molecule photosensitizer and chemical probe relay labelling. Nat Chem. 2024 Jun 4. doi: 10.1038/s41557-024-01545-6. Online ahead of print.
Mass spectrometry data entry: PXD042377