Details of the Probe

General Information of Probe

Probe ID
LDPC0003
Probe Name
Alkyne tyramide
Synonyms
Alkyne tyramide; 1694495-59-4; N-[2-(4-hydroxyphenyl)ethyl]pent-4-ynamide; N-(4-Hydroxyphenethyl)pent-4-ynamide; Alkyne-tyramide; SCHEMBL26268646; AKOS040759811; MS-23195; HY-131442; CS-0134719; G17521
Probe Type
ABPP Probe
Structure
3D MOL 2D MOL
Ro5 Violations (Lipinski): 0 Molecular Weight (mw) 217.26
Lipid-water partition coefficient (xlogp) 1.7
Rotatable Bond Count (rotbonds) 5
Hydrogen Bond Donor Count (hbonddonor) 2
Hydrogen Bond Acceptor Count (hbondacc) 2
Chemical Identifiers
Formula
C13H15NO2
IUPAC Name
N-[2-(4-hydroxyphenyl)ethyl]pent-4-ynamide
Canonical SMILES
C#CCCC(=O)NCCC1=CC=C(C=C1)O
InChI
InChI=1S/C13H15NO2/c1-2-3-4-13(16)14-10-9-11-5-7-12(15)8-6-11/h1,5-8,15H,3-4,9-10H2,(H,14,16)
InChIKey
CQZJBJSXQYZLEX-UHFFFAOYSA-N

The Probe Interaction Atlas

Target(s) List of this Probe

21 Enzyme Labeled by This Probe
Target Name Target ID Binding Site(Ratio) Interaction ID
Alpha-enolase (ENO1) LDTP02159 K330; K233  LDD0003 
Alpha-enolase (ENO1) LDTP02159 K330; K233  LDD0003 
Citrate synthase, mitochondrial (CS) LDTP01205 Y185; Y345  LDD0003 
Citrate synthase, mitochondrial (CS) LDTP01205 Y185; Y345  LDD0003 
Cytochrome b-c1 complex subunit 1, mitochondrial (UQCRC1) LDTP03610 Y123; Y420  LDD0003 
Cytochrome b-c1 complex subunit 1, mitochondrial (UQCRC1) LDTP03610 Y123; Y420  LDD0003 
Elongation factor 1-alpha 1 (EEF1A1) LDTP05071 K450; K180  LDD0003 
Elongation factor 1-alpha 1 (EEF1A1) LDTP05071 K450; K180  LDD0003 
Elongation factor G, mitochondrial (GFM1) LDTP10774 Y549; Y235  LDD0003 
Elongation factor G, mitochondrial (GFM1) LDTP10774 Y549; Y235  LDD0003 
Elongation factor Tu, mitochondrial (TUFM) LDTP04251 Y360; Y95  LDD0003 
Elongation factor Tu, mitochondrial (TUFM) LDTP04251 Y360; Y95  LDD0003 
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) LDTP02038 K117; K263  LDD0003 
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) LDTP02038 K117; K263  LDD0003 
GTP-binding nuclear protein Ran (RAN) LDTP05007 K37  LDD0003 
Isoleucine--tRNA ligase, mitochondrial (IARS2) LDTP12588 Y330; Y543; Y993  LDD0003 
Isoleucine--tRNA ligase, mitochondrial (IARS2) LDTP12588 Y330; Y543; Y993  LDD0003 
Isoleucine--tRNA ligase, mitochondrial (IARS2) LDTP12588 Y330; Y543; Y993  LDD0003 
Large ribosomal subunit protein mL39 (MRPL39) LDTP12835 Y235; Y211  LDD0003 
Large ribosomal subunit protein mL39 (MRPL39) LDTP12835 Y235; Y211  LDD0003 
Peptidyl-prolyl cis-trans isomerase A (PPIA) LDTP05028 K44  LDD0003 
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⏷ Show the Full List of 21 Enzyme
6 Transporter and channel Labeled by This Probe
Target Name Target ID Binding Site(Ratio) Interaction ID
ATP synthase subunit O, mitochondrial (ATP5PO) LDTP04150 Y35; Y46  LDD0003 
ATP synthase subunit O, mitochondrial (ATP5PO) LDTP04150 Y35; Y46  LDD0003 
Mitochondrial 2-oxoglutarate/malate carrier protein (SLC25A11) LDTP05384 Y94; Y259  LDD0003 
Mitochondrial 2-oxoglutarate/malate carrier protein (SLC25A11) LDTP05384 Y94; Y259  LDD0003 
Solute carrier family 25 member 3 (SLC25A3) LDTP05238 Y196; Y208  LDD0003 
Solute carrier family 25 member 3 (SLC25A3) LDTP05238 Y196; Y208  LDD0003 
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⏷ Show the Full List of 6 Transporter and channel
3 Transcription factor Labeled by This Probe
Target Name Target ID Binding Site(Ratio) Interaction ID
Transcription factor A, mitochondrial (TFAM) LDTP05235 Y200; Y103; Y162  LDD0003 
Transcription factor A, mitochondrial (TFAM) LDTP05235 Y200; Y103; Y162  LDD0003 
Transcription factor A, mitochondrial (TFAM) LDTP05235 Y200; Y103; Y162  LDD0003 
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21 Other Labeled by This Probe
Target Name Target ID Binding Site(Ratio) Interaction ID
10 kDa heat shock protein, mitochondrial (HSPE1) LDTP04931 Y76; Y88  LDD0003 
10 kDa heat shock protein, mitochondrial (HSPE1) LDTP04931 Y76; Y88  LDD0003 
Histone H1.4 (H1-4) LDTP02494 K64; K90; K75; K110  LDD0003 
Histone H1.4 (H1-4) LDTP02494 K64; K90; K75; K110  LDD0003 
Histone H1.4 (H1-4) LDTP02494 K64; K90; K75; K110  LDD0003 
Histone H1.4 (H1-4) LDTP02494 K64; K90; K75; K110  LDD0003 
Histone H2B type 2-F (H2BC18) LDTP07049 K117; K121; K47; K86  LDD0003 
Histone H2B type 2-F (H2BC18) LDTP07049 K117; K121; K47; K86  LDD0003 
Histone H2B type 2-F (H2BC18) LDTP07049 K117; K121; K47; K86  LDD0003 
Histone H2B type 2-F (H2BC18) LDTP07049 K117; K121; K47; K86  LDD0003 
Histone H3.3 (H3-3A; H3-3B) LDTP05198 K80; K57  LDD0003 
Histone H3.3 (H3-3A; H3-3B) LDTP05198 K80; K57  LDD0003 
Single-stranded DNA-binding protein, mitochondrial (SSBP1) LDTP05437 Y119; Y73  LDD0003 
Single-stranded DNA-binding protein, mitochondrial (SSBP1) LDTP05437 Y119; Y73  LDD0003 
Small ribosomal subunit protein uS17 (RPS11) LDTP04975 K147  LDD0003 
Stress-70 protein, mitochondrial (HSPA9) LDTP03860 Y128; Y652; Y568; Y181  LDD0003 
Stress-70 protein, mitochondrial (HSPA9) LDTP03860 Y128; Y652; Y568; Y181  LDD0003 
Stress-70 protein, mitochondrial (HSPA9) LDTP03860 Y128; Y652; Y568; Y181  LDD0003 
Stress-70 protein, mitochondrial (HSPA9) LDTP03860 Y128; Y652; Y568; Y181  LDD0003 
Stress-70 protein, mitochondrial (HSPA9) LDTP03860 Y128; Y652; Y568; Y181  LDD0003 
Transcription elongation regulator 1 (TCERG1) LDTP00430 K703  LDD0003 
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⏷ Show the Full List of 21 Other

Full Information of The Labelling Profiles of This Probe

Qualitation: Labeling site identification
  
Experiment 1 Reporting the Labelling Profiles of This Probe
REF [1]
Probe concentration
5 mM
Experiment Method
Proximity labeling
In Vitro Experiment Model
Model Type
Living cell
Derived Tissue
Peripheral blood
Disease Model
Normal
Model Name
Human normal cells (HEK293T-APEX-mito)
Interaction Atlas ID
 LDD0003 
Download The Altas
  
Experiment 2 Reporting the Labelling Profiles of This Probe
REF [1]
Probe concentration
2 mM
Experiment Method
Proximity labeling
In Vitro Experiment Model
Model Type
Living cell
Derived Tissue
Cervix uteri
Disease Model
Endocervical adenocarcinoma [ICD-11:2C77]
Model Name
Human endocervical adenocarcinoma cells (HeLa)
Interaction Atlas ID
 LDD0012 
Download The Altas

References

1 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764