General Information of Experiment

Probe Name MCL-4  Probe Info 
Probe concentration 10 uM
Criteria Quantification: Probe vs (Probe+Competitor)
Quantitative Method
LFQ
Competitor Name Micheliolide  Competitor Info 
Competitor Concentration 10 uM
In Vivo Experiment Model Model Type Cell lysate
Derived Tissue Peripheral blood
Disease Model Normal
Model Name Human normal cell lysate (M9-ENL1)

Interaction Altas of This Experiment [1]

Enzyme
Click to Show/Hide to Show 10 Protein Related to This Bioclass
Target ID Target Name Binding Ratio
 LDTP00717  Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1 (PAPSS1) 7.6
 LDTP04892  Ras-related protein Rab-2A (RAB2A) 7.0
 LDTP09258  ATP-dependent RNA helicase DDX55 (DDX55) 5.5
 LDTP10358  Probable ATP-dependent RNA helicase DDX27 (DDX27) 3.2
 LDTP08254  Ubiquitin carboxyl-terminal hydrolase 48 (USP48) 2.2
 LDTP08082  L-xylulose reductase (DCXR) 1.8
 LDTP04910  Ras-related protein Rap-1b (RAP1B) 1.7
 LDTP04334  DNA ligase 3 (LIG3) 1.6
 LDTP04901  Ras-related protein Rab-14 (RAB14) 1.6
 LDTP01707  Thioredoxin domain-containing protein 12 (TXNDC12) 1.5
Transporter and channel
Click to Show/Hide to Show 1 Protein Related to This Bioclass
Target ID Target Name Binding Ratio
 LDTP02010  Annexin A1 (ANXA1) 2.3
Other
Click to Show/Hide to Show 11 Protein Related to This Bioclass
Target ID Target Name Binding Ratio
 LDTP00552  Actin-related protein 2/3 complex subunit 3 (ARPC3) 7.4
 LDTP03555  Guanine nucleotide-binding protein subunit alpha-15 (GNA15) 4.2
 LDTP06562  Histone-binding protein RBBP7 (RBBP7) 3.2
 LDTP01036  Leupaxin (LPXN) 2.5
 LDTP02365  U2 small nuclear ribonucleoprotein A' (SNRPA1) 2.2
 LDTP01683  BAG family molecular chaperone regulator 2 (BAG2) 1.9
 LDTP05015  Small ribosomal subunit protein eS28 (RPS28) 1.6
 LDTP04995  CCHC-type zinc finger nucleic acid binding protein (CNBP) 1.5
 LDTP06176  Mediator of DNA damage checkpoint protein 1 (MDC1) 1.5
 LDTP13167  Peflin (PEF1) 1.5
 LDTP04504  Rap1 GTPase-GDP dissociation stimulator 1 (RAP1GDS1) 1.5

References

1 Comprehensive Structure-Activity Profiling of Micheliolide and its Targeted Proteome in Leukemia Cells via Probe-Guided Late-Stage C-H Functionalization. ACS Cent Sci. 2021 May 26;7(5):841-857. doi: 10.1021/acscentsci.0c01624. Epub 2021 Apr 28.
Mass spectrometry data entry: PXD024455