Details of the Target

General Information of Target

Target ID LDTP12311
Target Name Carbohydrate-responsive element-binding protein (MLXIPL)
Gene Name MLXIPL
Gene ID 51085
Synonyms
BHLHD14; MIO; WBSCR14; Carbohydrate-responsive element-binding protein; ChREBP; Class D basic helix-loop-helix protein 14; bHLHd14; MLX interactor; MLX-interacting protein-like; WS basic-helix-loop-helix leucine zipper protein; WS-bHLH; Williams-Beuren syndrome chromosomal region 14 protein
3D Structure
Download
2D Sequence (FASTA)
Download
3D Structure (PDB)
Download
Sequence
MSASKIPLFKMKDLILILCLLEMSFAVPFFPQQSGTPGMASLSLETMRQLGSLQRLNTLS
QYSRYGFGKSFNSLWMHGLLPPHSSLPWMRPREHETQQYEYSLPVHPPPLPSQPSLKPQQ
PGLKPFLQSAAATTNQATALKEALQPPIHLGHLPLQEGELPLVQQQVAPSDKPPKPELPG
VDFADPQGPSLPGMDFPDPQGPSLPGLDFADPQGSTIFQIARLISHGPMPQNKQSPLYPG
MLYVPFGANQLNAPARLGIMSSEEVAGGREDPMAYGAMFPGFGGMRPGFEGMPHNPAMGG
DFTLEFDSPVAATKGPENEEGGAQGSPMPEANPDNLENPAFLTELEPAPHAGLLALPKDD
IPGLPRSPSGKMKGLPSVTPAAADPLMTPELADVYRTYDADMTTSVDFQEEATMDTTMAP
NSLQTSMPGNKAQEPEMMHDAWHFQEP
Target Bioclass
Transcription factor
Subcellular location
Nucleus
Function
Binds DNA as a heterodimer with MLX/TCFL4 and activates transcription. Binds to the canonical E box sequence 5'-CACGTG-3'. Plays a role in transcriptional activation of glycolytic target genes. Involved in glucose-responsive gene regulation. Regulates transcription in response to changes in cellular carbohydrate abundance such as occurs during fasting to feeding metabolic transition. Refeeding stimulates MLXIPL/ChREBP transcription factor, leading to increased BCKDK to PPM1K expression ratio, phosphorylation and activation of ACLY that ultimately results in the generation of malonyl-CoA and oxaloacetate immediate substrates of de novo lipogenesis and gluconeogenesis, respectively.
Uniprot ID
Q9NP71
Ensemble ID
ENST00000313375.8
HGNC ID
HGNC:12744

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
A549 SNV: p.A598S .
COLO792 SNV: p.F768L .
HEC1 SNV: p.R761C .
HEC1B SNV: p.R761C .
IGROV1 Deletion: p.L149CfsTer29 .
Ishikawa (Heraklio) 02 ER SNV: p.V546I .
MDAMB436 SNV: p.D22E .
MFE319 SNV: p.L366V; p.R718W .
MOLT4 SNV: p.R834H .
NCIH358 SNV: p.T533S .
RL952 SNV: p.T147I .
RT112 SNV: p.P436A .
TOV21G SNV: p.E175K .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 2 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C143(1.44); C218(0.77)  LDD0532  [1]
TFBX
 Probe Info  		N.A.  LDD0148  [2]
PAL-AfBPP Probe
Click To Hide/Show 1 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
Alk-rapa
 Probe Info  		7.64  LDD0213  [3]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0215  AC10 HCT 116 C143(1.44); C218(0.77)  LDD0532  [1]
 LDCM0226  AC11 HCT 116 C143(1.26); C218(1.04)  LDD0543  [1]
 LDCM0237  AC12 HCT 116 C143(1.10); C218(0.94)  LDD0554  [1]
 LDCM0259  AC14 HCT 116 C143(1.38); C218(0.78)  LDD0576  [1]
 LDCM0263  AC143 HCT 116 C143(0.95); C218(1.20)  LDD0580  [1]
 LDCM0264  AC144 HCT 116 C143(0.93); C218(1.08)  LDD0581  [1]
 LDCM0265  AC145 HCT 116 C143(0.74); C218(1.15)  LDD0582  [1]
 LDCM0266  AC146 HCT 116 C143(0.90); C218(1.30)  LDD0583  [1]
 LDCM0267  AC147 HCT 116 C218(1.01); C143(1.02)  LDD0584  [1]
 LDCM0268  AC148 HCT 116 C143(0.73); C218(3.20)  LDD0585  [1]
 LDCM0269  AC149 HCT 116 C143(0.93); C218(2.34)  LDD0586  [1]
 LDCM0270  AC15 HCT 116 C218(0.77); C143(1.35)  LDD0587  [1]
 LDCM0271  AC150 HCT 116 C143(0.99); C218(1.22)  LDD0588  [1]
 LDCM0272  AC151 HCT 116 C143(0.90); C218(1.12)  LDD0589  [1]
 LDCM0273  AC152 HCT 116 C143(0.99); C218(1.19)  LDD0590  [1]
 LDCM0274  AC153 HCT 116 C143(0.80); C218(1.97)  LDD0591  [1]
 LDCM0621  AC154 HCT 116 C143(1.10); C218(0.92)  LDD2158  [1]
 LDCM0622  AC155 HCT 116 C143(0.87); C218(1.24)  LDD2159  [1]
 LDCM0623  AC156 HCT 116 C143(0.89); C218(1.02)  LDD2160  [1]
 LDCM0624  AC157 HCT 116 C143(0.90); C218(1.18)  LDD2161  [1]
 LDCM0276  AC17 HCT 116 C143(1.11); C218(1.11)  LDD0593  [1]
 LDCM0277  AC18 HCT 116 C143(0.95); C218(1.17)  LDD0594  [1]
 LDCM0278  AC19 HCT 116 C143(0.89); C218(1.08)  LDD0595  [1]
 LDCM0280  AC20 HCT 116 C143(0.86); C218(0.99)  LDD0597  [1]
 LDCM0281  AC21 HCT 116 C143(0.84); C218(1.22)  LDD0598  [1]
 LDCM0282  AC22 HCT 116 C218(0.93); C143(1.04)  LDD0599  [1]
 LDCM0283  AC23 HCT 116 C143(0.85); C218(1.11)  LDD0600  [1]
 LDCM0284  AC24 HCT 116 C143(0.89); C218(1.04)  LDD0601  [1]
 LDCM0285  AC25 HCT 116 C218(0.87)  LDD0602  [1]
 LDCM0286  AC26 HCT 116 C218(1.01)  LDD0603  [1]
 LDCM0287  AC27 HCT 116 C218(1.06)  LDD0604  [1]
 LDCM0288  AC28 HCT 116 C218(1.34)  LDD0605  [1]
 LDCM0289  AC29 HCT 116 C218(1.08)  LDD0606  [1]
 LDCM0291  AC30 HCT 116 C218(1.11)  LDD0608  [1]
 LDCM0292  AC31 HCT 116 C218(1.02)  LDD0609  [1]
 LDCM0293  AC32 HCT 116 C218(1.18)  LDD0610  [1]
 LDCM0294  AC33 HCT 116 C218(0.97)  LDD0611  [1]
 LDCM0295  AC34 HCT 116 C218(0.92)  LDD0612  [1]
 LDCM0296  AC35 HCT 116 C218(0.07)  LDD0613  [1]
 LDCM0297  AC36 HCT 116 C218(1.01)  LDD0614  [1]
 LDCM0298  AC37 HCT 116 C218(1.02)  LDD0615  [1]
 LDCM0299  AC38 HCT 116 C218(0.70)  LDD0616  [1]
 LDCM0300  AC39 HCT 116 C218(0.99)  LDD0617  [1]
 LDCM0302  AC40 HCT 116 C218(0.85)  LDD0619  [1]
 LDCM0303  AC41 HCT 116 C218(0.56)  LDD0620  [1]
 LDCM0304  AC42 HCT 116 C218(0.94)  LDD0621  [1]
 LDCM0305  AC43 HCT 116 C218(1.04)  LDD0622  [1]
 LDCM0306  AC44 HCT 116 C218(0.89)  LDD0623  [1]
 LDCM0307  AC45 HCT 116 C218(0.88)  LDD0624  [1]
 LDCM0308  AC46 HCT 116 C218(1.10)  LDD0625  [1]
 LDCM0309  AC47 HCT 116 C218(0.98)  LDD0626  [1]
 LDCM0310  AC48 HCT 116 C218(1.04)  LDD0627  [1]
 LDCM0311  AC49 HCT 116 C218(1.32)  LDD0628  [1]
 LDCM0313  AC50 HCT 116 C218(1.35)  LDD0630  [1]
 LDCM0314  AC51 HCT 116 C218(0.92)  LDD0631  [1]
 LDCM0315  AC52 HCT 116 C218(1.02)  LDD0632  [1]
 LDCM0316  AC53 HCT 116 C218(1.41)  LDD0633  [1]
 LDCM0317  AC54 HCT 116 C218(1.10)  LDD0634  [1]
 LDCM0318  AC55 HCT 116 C218(1.18)  LDD0635  [1]
 LDCM0319  AC56 HCT 116 C218(1.39)  LDD0636  [1]
 LDCM0320  AC57 HCT 116 C143(1.08)  LDD0637  [1]
 LDCM0321  AC58 HCT 116 C143(0.95)  LDD0638  [1]
 LDCM0322  AC59 HCT 116 C143(1.25)  LDD0639  [1]
 LDCM0323  AC6 HCT 116 C218(0.90); C143(1.36)  LDD0640  [1]
 LDCM0324  AC60 HCT 116 C143(1.13)  LDD0641  [1]
 LDCM0325  AC61 HCT 116 C143(1.16)  LDD0642  [1]
 LDCM0326  AC62 HCT 116 C143(1.26)  LDD0643  [1]
 LDCM0327  AC63 HCT 116 C143(1.04)  LDD0644  [1]
 LDCM0328  AC64 HCT 116 C143(1.10)  LDD0645  [1]
 LDCM0329  AC65 HCT 116 C143(0.98)  LDD0646  [1]
 LDCM0330  AC66 HCT 116 C143(0.97)  LDD0647  [1]
 LDCM0331  AC67 HCT 116 C143(1.08)  LDD0648  [1]
 LDCM0334  AC7 HCT 116 C218(0.82); C143(1.22)  LDD0651  [1]
 LDCM0345  AC8 HCT 116 C218(0.85); C143(1.29)  LDD0662  [1]
 LDCM0349  AC83 HCT 116 C218(1.82)  LDD0666  [1]
 LDCM0350  AC84 HCT 116 C218(1.72)  LDD0667  [1]
 LDCM0351  AC85 HCT 116 C218(1.52)  LDD0668  [1]
 LDCM0352  AC86 HCT 116 C218(1.32)  LDD0669  [1]
 LDCM0353  AC87 HCT 116 C218(1.09)  LDD0670  [1]
 LDCM0354  AC88 HCT 116 C218(1.18)  LDD0671  [1]
 LDCM0355  AC89 HCT 116 C218(1.38)  LDD0672  [1]
 LDCM0357  AC90 HCT 116 C218(0.92)  LDD0674  [1]
 LDCM0358  AC91 HCT 116 C218(1.85)  LDD0675  [1]
 LDCM0359  AC92 HCT 116 C218(1.72)  LDD0676  [1]
 LDCM0360  AC93 HCT 116 C218(1.17)  LDD0677  [1]
 LDCM0361  AC94 HCT 116 C218(1.21)  LDD0678  [1]
 LDCM0362  AC95 HCT 116 C218(1.08)  LDD0679  [1]
 LDCM0363  AC96 HCT 116 C218(1.37)  LDD0680  [1]
 LDCM0364  AC97 HCT 116 C218(1.60)  LDD0681  [1]
 LDCM0248  AKOS034007472 HCT 116 C143(1.29); C218(0.90)  LDD0565  [1]
 LDCM0356  AKOS034007680 HCT 116 C218(0.90); C143(1.21)  LDD0673  [1]
 LDCM0275  AKOS034007705 HCT 116 C218(0.71); C143(1.61)  LDD0592  [1]
 LDCM0370  CL101 HCT 116 C218(0.94); C143(1.21)  LDD0687  [1]
 LDCM0371  CL102 HCT 116 C218(1.03); C143(1.08)  LDD0688  [1]
 LDCM0372  CL103 HCT 116 C218(0.84); C143(1.10)  LDD0689  [1]
 LDCM0373  CL104 HCT 116 C218(0.87); C143(1.34)  LDD0690  [1]
 LDCM0374  CL105 HCT 116 C143(1.01); C218(1.30)  LDD0691  [1]
 LDCM0375  CL106 HCT 116 C143(1.01); C218(1.43)  LDD0692  [1]
 LDCM0376  CL107 HCT 116 C143(0.94); C218(1.30)  LDD0693  [1]
 LDCM0377  CL108 HCT 116 C143(0.98); C218(1.46)  LDD0694  [1]
 LDCM0378  CL109 HCT 116 C143(0.96); C218(1.41)  LDD0695  [1]
 LDCM0380  CL110 HCT 116 C143(1.18); C218(1.95)  LDD0697  [1]
 LDCM0381  CL111 HCT 116 C143(0.94); C218(1.40)  LDD0698  [1]
 LDCM0382  CL112 HCT 116 C218(1.09)  LDD0699  [1]
 LDCM0383  CL113 HCT 116 C218(1.04)  LDD0700  [1]
 LDCM0384  CL114 HCT 116 C218(1.18)  LDD0701  [1]
 LDCM0385  CL115 HCT 116 C218(1.07)  LDD0702  [1]
 LDCM0386  CL116 HCT 116 C218(1.04)  LDD0703  [1]
 LDCM0387  CL117 HCT 116 C218(1.00)  LDD0704  [1]
 LDCM0388  CL118 HCT 116 C218(1.02)  LDD0705  [1]
 LDCM0389  CL119 HCT 116 C218(1.09)  LDD0706  [1]
 LDCM0391  CL120 HCT 116 C218(1.02)  LDD0708  [1]
 LDCM0392  CL121 HCT 116 C218(0.89)  LDD0709  [1]
 LDCM0393  CL122 HCT 116 C218(1.19)  LDD0710  [1]
 LDCM0394  CL123 HCT 116 C218(2.28)  LDD0711  [1]
 LDCM0395  CL124 HCT 116 C218(1.54)  LDD0712  [1]
 LDCM0396  CL125 HCT 116 C143(1.11)  LDD0713  [1]
 LDCM0397  CL126 HCT 116 C143(1.21)  LDD0714  [1]
 LDCM0398  CL127 HCT 116 C143(1.01)  LDD0715  [1]
 LDCM0399  CL128 HCT 116 C143(0.92)  LDD0716  [1]
 LDCM0436  CL46 HCT 116 C143(0.80); C218(0.80)  LDD0753  [1]
 LDCM0437  CL47 HCT 116 C143(1.05); C218(0.95)  LDD0754  [1]
 LDCM0438  CL48 HCT 116 C143(0.88); C218(0.84)  LDD0755  [1]
 LDCM0439  CL49 HCT 116 C143(0.93); C218(0.93)  LDD0756  [1]
 LDCM0441  CL50 HCT 116 C143(1.13); C218(0.98)  LDD0758  [1]
 LDCM0442  CL51 HCT 116 C143(1.02); C218(0.85)  LDD0759  [1]
 LDCM0443  CL52 HCT 116 C143(1.00); C218(0.92)  LDD0760  [1]
 LDCM0444  CL53 HCT 116 C143(0.82); C218(1.07)  LDD0761  [1]
 LDCM0445  CL54 HCT 116 C143(0.86); C218(0.93)  LDD0762  [1]
 LDCM0446  CL55 HCT 116 C143(0.80); C218(0.78)  LDD0763  [1]
 LDCM0447  CL56 HCT 116 C143(1.02); C218(0.97)  LDD0764  [1]
 LDCM0448  CL57 HCT 116 C143(0.93); C218(1.11)  LDD0765  [1]
 LDCM0449  CL58 HCT 116 C143(0.81); C218(0.84)  LDD0766  [1]
 LDCM0450  CL59 HCT 116 C143(0.99); C218(1.02)  LDD0767  [1]
 LDCM0452  CL60 HCT 116 C143(1.01); C218(1.10)  LDD0769  [1]
 LDCM0453  CL61 HCT 116 C218(1.11)  LDD0770  [1]
 LDCM0454  CL62 HCT 116 C218(1.17)  LDD0771  [1]
 LDCM0455  CL63 HCT 116 C218(1.13)  LDD0772  [1]
 LDCM0456  CL64 HCT 116 C218(1.69)  LDD0773  [1]
 LDCM0457  CL65 HCT 116 C218(1.16)  LDD0774  [1]
 LDCM0458  CL66 HCT 116 C218(1.77)  LDD0775  [1]
 LDCM0459  CL67 HCT 116 C218(1.42)  LDD0776  [1]
 LDCM0460  CL68 HCT 116 C218(1.52)  LDD0777  [1]
 LDCM0461  CL69 HCT 116 C218(1.40)  LDD0778  [1]
 LDCM0463  CL70 HCT 116 C218(1.22)  LDD0780  [1]
 LDCM0464  CL71 HCT 116 C218(1.38)  LDD0781  [1]
 LDCM0465  CL72 HCT 116 C218(1.14)  LDD0782  [1]
 LDCM0466  CL73 HCT 116 C218(1.48)  LDD0783  [1]
 LDCM0467  CL74 HCT 116 C218(1.31)  LDD0784  [1]
 LDCM0469  CL76 HCT 116 C218(0.33)  LDD0786  [1]
 LDCM0470  CL77 HCT 116 C218(0.93)  LDD0787  [1]
 LDCM0471  CL78 HCT 116 C218(1.52)  LDD0788  [1]
 LDCM0472  CL79 HCT 116 C218(0.72)  LDD0789  [1]
 LDCM0474  CL80 HCT 116 C218(0.92)  LDD0791  [1]
 LDCM0475  CL81 HCT 116 C218(0.76)  LDD0792  [1]
 LDCM0476  CL82 HCT 116 C218(0.78)  LDD0793  [1]
 LDCM0477  CL83 HCT 116 C218(1.03)  LDD0794  [1]
 LDCM0478  CL84 HCT 116 C218(1.09)  LDD0795  [1]
 LDCM0479  CL85 HCT 116 C218(0.92)  LDD0796  [1]
 LDCM0480  CL86 HCT 116 C218(0.58)  LDD0797  [1]
 LDCM0481  CL87 HCT 116 C218(0.90)  LDD0798  [1]
 LDCM0482  CL88 HCT 116 C218(0.75)  LDD0799  [1]
 LDCM0483  CL89 HCT 116 C218(0.86)  LDD0800  [1]
 LDCM0485  CL90 HCT 116 C218(1.49)  LDD0802  [1]
 LDCM0468  Fragment33 HCT 116 C218(1.44)  LDD0785  [1]
 LDCM0090  Rapamycin JHH-7 7.64  LDD0213  [3]

References

1 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
2 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
3 Rapamycin targets STAT3 and impacts c-Myc to suppress tumor growth. Cell Chem Biol. 2022 Mar 17;29(3):373-385.e6. doi: 10.1016/j.chembiol.2021.10.006. Epub 2021 Oct 26.