Details of the Target

General Information of Target

Target ID LDTP11967
Target Name Probable ATP-dependent RNA helicase DHX35 (DHX35)
Gene Name DHX35
Gene ID 60625
Synonyms
C20orf15; DDX35; Probable ATP-dependent RNA helicase DHX35; EC 3.6.4.13; DEAH box protein 35
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MKLWIHLFYSSLLACISLHSQTPVLSSRGSCDSLCNCEEKDGTMLINCEAKGIKMVSEIS
VPPSRPFQLSLLNNGLTMLHTNDFSGLTNAISIHLGFNNIADIEIGAFNGLGLLKQLHIN
HNSLEILKEDTFHGLENLEFLQADNNFITVIEPSAFSKLNRLKVLILNDNAIESLPPNIF
RFVPLTHLDLRGNQLQTLPYVGFLEHIGRILDLQLEDNKWACNCDLLQLKTWLENMPPQS
IIGDVVCNSPPFFKGSILSRLKKESICPTPPVYEEHEDPSGSLHLAATSSINDSRMSTKT
TSILKLPTKAPGLIPYITKPSTQLPGPYCPIPCNCKVLSPSGLLIHCQERNIESLSDLRP
PPQNPRKLILAGNIIHSLMKSDLVEYFTLEMLHLGNNRIEVLEEGSFMNLTRLQKLYLNG
NHLTKLSKGMFLGLHNLEYLYLEYNAIKEILPGTFNPMPKLKVLYLNNNLLQVLPPHIFS
GVPLTKVNLKTNQFTHLPVSNILDDLDLLTQIDLEDNPWDCSCDLVGLQQWIQKLSKNTV
TDDILCTSPGHLDKKELKALNSEILCPGLVNNPSMPTQTSYLMVTTPATTTNTADTILRS
LTDAVPLSVLILGLLIMFITIVFCAAGIVVLVLHRRRRYKKKQVDEQMRDNSPVHLQYSM
YGHKTTHHTTERPSASLYEQHMVSPMVHVYRSPSFGPKHLEEEEERNEKEGSDAKHLQRS
LLEQENHSPLTGSNMKYKTTNQSTEFLSFQDASSLYRNILEKERELQQLGITEYLRKNIA
QLQPDMEAHYPGAHEELKLMETLMYSRPRKVLVEQTKNEYFELKANLHAEPDYLEVLEQQ
T
Target Bioclass
Enzyme
Family
DEAD box helicase family, DEAH subfamily
Function May be involved in pre-mRNA splicing.
Uniprot ID
Q9H5Z1
Ensemble ID
ENST00000252011.8
HGNC ID
HGNC:15861

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
L428 SNV: p.I358T .
TOV21G SNV: p.S292L DBIA    Probe Info 

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 6 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
IPM
 Probe Info  		C141(0.00); C135(0.00)  LDD0241  [1]
DBIA
 Probe Info  		C609(0.93)  LDD0531  [2]
4-Iodoacetamidophenylacetylene
 Probe Info  		C473(0.00); C135(0.00); C141(0.00)  LDD0038  [3]
IA-alkyne
 Probe Info  		C473(0.00); C565(0.00); C609(0.00)  LDD0036  [3]
Lodoacetamide azide
 Probe Info  		C609(0.00); C473(0.00); C135(0.00); C565(0.00)  LDD0037  [3]
AOyne
 Probe Info  		9.10  LDD0443  [4]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0214  AC1 HCT 116 C609(0.93)  LDD0531  [2]
 LDCM0215  AC10 PaTu 8988t C135(0.87)  LDD1094  [2]
 LDCM0226  AC11 PaTu 8988t C135(0.90)  LDD1105  [2]
 LDCM0237  AC12 PaTu 8988t C135(0.97)  LDD1116  [2]
 LDCM0246  AC128 PaTu 8988t C135(0.76)  LDD1125  [2]
 LDCM0247  AC129 PaTu 8988t C135(0.87)  LDD1126  [2]
 LDCM0249  AC130 PaTu 8988t C135(0.89)  LDD1128  [2]
 LDCM0250  AC131 PaTu 8988t C135(1.00)  LDD1129  [2]
 LDCM0251  AC132 PaTu 8988t C135(0.87)  LDD1130  [2]
 LDCM0252  AC133 PaTu 8988t C135(1.07)  LDD1131  [2]
 LDCM0253  AC134 PaTu 8988t C135(0.97)  LDD1132  [2]
 LDCM0254  AC135 PaTu 8988t C135(0.82)  LDD1133  [2]
 LDCM0255  AC136 PaTu 8988t C135(0.87)  LDD1134  [2]
 LDCM0256  AC137 PaTu 8988t C135(0.84)  LDD1135  [2]
 LDCM0257  AC138 PaTu 8988t C135(0.77)  LDD1136  [2]
 LDCM0258  AC139 PaTu 8988t C135(0.79)  LDD1137  [2]
 LDCM0259  AC14 PaTu 8988t C135(0.88)  LDD1138  [2]
 LDCM0260  AC140 PaTu 8988t C135(0.68)  LDD1139  [2]
 LDCM0261  AC141 PaTu 8988t C135(0.95)  LDD1140  [2]
 LDCM0262  AC142 PaTu 8988t C135(0.95)  LDD1141  [2]
 LDCM0263  AC143 PaTu 8988t C135(1.04)  LDD1142  [2]
 LDCM0264  AC144 PaTu 8988t C135(1.00)  LDD1143  [2]
 LDCM0265  AC145 PaTu 8988t C135(1.10)  LDD1144  [2]
 LDCM0266  AC146 PaTu 8988t C135(0.95)  LDD1145  [2]
 LDCM0267  AC147 PaTu 8988t C135(1.22)  LDD1146  [2]
 LDCM0268  AC148 PaTu 8988t C135(1.16)  LDD1147  [2]
 LDCM0269  AC149 PaTu 8988t C135(1.10)  LDD1148  [2]
 LDCM0270  AC15 PaTu 8988t C135(0.93)  LDD1149  [2]
 LDCM0271  AC150 PaTu 8988t C135(1.22)  LDD1150  [2]
 LDCM0272  AC151 PaTu 8988t C135(1.16)  LDD1151  [2]
 LDCM0273  AC152 PaTu 8988t C135(1.02)  LDD1152  [2]
 LDCM0274  AC153 PaTu 8988t C135(1.15)  LDD1153  [2]
 LDCM0621  AC154 PaTu 8988t C135(1.11)  LDD2166  [2]
 LDCM0622  AC155 PaTu 8988t C135(1.06)  LDD2167  [2]
 LDCM0623  AC156 PaTu 8988t C135(1.02)  LDD2168  [2]
 LDCM0624  AC157 PaTu 8988t C135(1.15)  LDD2169  [2]
 LDCM0276  AC17 PaTu 8988t C141(0.89)  LDD1155  [2]
 LDCM0277  AC18 PaTu 8988t C141(1.26)  LDD1156  [2]
 LDCM0278  AC19 PaTu 8988t C141(1.00)  LDD1157  [2]
 LDCM0279  AC2 HCT 116 C609(1.08)  LDD0596  [2]
 LDCM0280  AC20 PaTu 8988t C141(1.08)  LDD1159  [2]
 LDCM0281  AC21 PaTu 8988t C141(1.18)  LDD1160  [2]
 LDCM0282  AC22 PaTu 8988t C141(1.26)  LDD1161  [2]
 LDCM0283  AC23 PaTu 8988t C141(1.09)  LDD1162  [2]
 LDCM0284  AC24 PaTu 8988t C141(0.91)  LDD1163  [2]
 LDCM0285  AC25 HCT 116 C609(0.93)  LDD0602  [2]
 LDCM0286  AC26 HCT 116 C609(1.06)  LDD0603  [2]
 LDCM0287  AC27 HCT 116 C609(0.98)  LDD0604  [2]
 LDCM0288  AC28 HCT 116 C609(0.96)  LDD0605  [2]
 LDCM0289  AC29 HCT 116 C609(1.22)  LDD0606  [2]
 LDCM0290  AC3 HCT 116 C609(1.00)  LDD0607  [2]
 LDCM0291  AC30 HCT 116 C609(1.04)  LDD0608  [2]
 LDCM0292  AC31 HCT 116 C609(0.92)  LDD0609  [2]
 LDCM0293  AC32 HCT 116 C609(1.16)  LDD0610  [2]
 LDCM0294  AC33 HCT 116 C609(0.99)  LDD0611  [2]
 LDCM0295  AC34 HCT 116 C609(1.52)  LDD0612  [2]
 LDCM0296  AC35 HCT 116 C609(0.85)  LDD0613  [2]
 LDCM0297  AC36 HCT 116 C609(0.95)  LDD0614  [2]
 LDCM0298  AC37 HCT 116 C609(1.03)  LDD0615  [2]
 LDCM0299  AC38 HCT 116 C609(0.94)  LDD0616  [2]
 LDCM0300  AC39 HCT 116 C609(0.88)  LDD0617  [2]
 LDCM0301  AC4 HCT 116 C609(0.92)  LDD0618  [2]
 LDCM0302  AC40 HCT 116 C609(1.25)  LDD0619  [2]
 LDCM0303  AC41 HCT 116 C609(1.05)  LDD0620  [2]
 LDCM0304  AC42 HCT 116 C609(0.99)  LDD0621  [2]
 LDCM0305  AC43 HCT 116 C609(0.98)  LDD0622  [2]
 LDCM0306  AC44 HCT 116 C609(1.03)  LDD0623  [2]
 LDCM0307  AC45 HCT 116 C609(1.05)  LDD0624  [2]
 LDCM0308  AC46 HCT 116 C609(1.08)  LDD0625  [2]
 LDCM0309  AC47 HCT 116 C609(1.03)  LDD0626  [2]
 LDCM0310  AC48 HCT 116 C609(0.72)  LDD0627  [2]
 LDCM0311  AC49 HCT 116 C609(0.71)  LDD0628  [2]
 LDCM0312  AC5 HCT 116 C609(0.87)  LDD0629  [2]
 LDCM0313  AC50 HCT 116 C609(0.70)  LDD0630  [2]
 LDCM0314  AC51 HCT 116 C609(0.89)  LDD0631  [2]
 LDCM0315  AC52 HCT 116 C609(0.86)  LDD0632  [2]
 LDCM0316  AC53 HCT 116 C609(0.80)  LDD0633  [2]
 LDCM0317  AC54 HCT 116 C609(0.78)  LDD0634  [2]
 LDCM0318  AC55 HCT 116 C609(0.73)  LDD0635  [2]
 LDCM0319  AC56 HCT 116 C609(0.88)  LDD0636  [2]
 LDCM0320  AC57 HCT 116 C141(0.79)  LDD0637  [2]
 LDCM0321  AC58 HCT 116 C141(0.82)  LDD0638  [2]
 LDCM0322  AC59 HCT 116 C141(0.81)  LDD0639  [2]
 LDCM0323  AC6 PaTu 8988t C135(0.92)  LDD1202  [2]
 LDCM0324  AC60 HCT 116 C141(0.77)  LDD0641  [2]
 LDCM0325  AC61 HCT 116 C141(0.92)  LDD0642  [2]
 LDCM0326  AC62 HCT 116 C141(0.96)  LDD0643  [2]
 LDCM0327  AC63 HCT 116 C141(0.90)  LDD0644  [2]
 LDCM0328  AC64 HCT 116 C141(0.82)  LDD0645  [2]
 LDCM0329  AC65 HCT 116 C141(1.23)  LDD0646  [2]
 LDCM0330  AC66 HCT 116 C141(1.17)  LDD0647  [2]
 LDCM0331  AC67 HCT 116 C141(0.87)  LDD0648  [2]
 LDCM0332  AC68 HCT 116 C141(0.63)  LDD0649  [2]
 LDCM0333  AC69 HCT 116 C141(0.61)  LDD0650  [2]
 LDCM0334  AC7 PaTu 8988t C135(0.97)  LDD1213  [2]
 LDCM0335  AC70 HCT 116 C141(0.42)  LDD0652  [2]
 LDCM0336  AC71 HCT 116 C141(0.52)  LDD0653  [2]
 LDCM0337  AC72 HCT 116 C141(0.91)  LDD0654  [2]
 LDCM0338  AC73 HCT 116 C141(0.53)  LDD0655  [2]
 LDCM0339  AC74 HCT 116 C141(0.59)  LDD0656  [2]
 LDCM0340  AC75 HCT 116 C141(0.55)  LDD0657  [2]
 LDCM0341  AC76 HCT 116 C141(0.47)  LDD0658  [2]
 LDCM0342  AC77 HCT 116 C141(0.64)  LDD0659  [2]
 LDCM0343  AC78 HCT 116 C141(0.69)  LDD0660  [2]
 LDCM0344  AC79 HCT 116 C141(0.65)  LDD0661  [2]
 LDCM0345  AC8 PaTu 8988t C135(0.81)  LDD1224  [2]
 LDCM0346  AC80 HCT 116 C141(0.83)  LDD0663  [2]
 LDCM0347  AC81 HCT 116 C141(0.46)  LDD0664  [2]
 LDCM0348  AC82 HCT 116 C141(0.49)  LDD0665  [2]
 LDCM0349  AC83 HCT 116 C141(0.81)  LDD0666  [2]
 LDCM0350  AC84 HCT 116 C141(0.64)  LDD0667  [2]
 LDCM0351  AC85 HCT 116 C141(0.73)  LDD0668  [2]
 LDCM0352  AC86 HCT 116 C141(0.82)  LDD0669  [2]
 LDCM0353  AC87 HCT 116 C141(0.65)  LDD0670  [2]
 LDCM0354  AC88 HCT 116 C141(0.78)  LDD0671  [2]
 LDCM0355  AC89 HCT 116 C141(0.77)  LDD0672  [2]
 LDCM0357  AC90 HCT 116 C141(1.42)  LDD0674  [2]
 LDCM0358  AC91 HCT 116 C141(0.72)  LDD0675  [2]
 LDCM0359  AC92 HCT 116 C141(0.68)  LDD0676  [2]
 LDCM0360  AC93 HCT 116 C141(0.88)  LDD0677  [2]
 LDCM0361  AC94 HCT 116 C141(0.58)  LDD0678  [2]
 LDCM0362  AC95 HCT 116 C141(0.72)  LDD0679  [2]
 LDCM0363  AC96 HCT 116 C141(0.81)  LDD0680  [2]
 LDCM0364  AC97 HCT 116 C141(0.70)  LDD0681  [2]
 LDCM0248  AKOS034007472 PaTu 8988t C135(0.96)  LDD1127  [2]
 LDCM0356  AKOS034007680 PaTu 8988t C135(0.85)  LDD1235  [2]
 LDCM0275  AKOS034007705 PaTu 8988t C135(0.78)  LDD1154  [2]
 LDCM0369  CL100 HCT 116 C609(1.00)  LDD0686  [2]
 LDCM0370  CL101 PaTu 8988t C135(1.12)  LDD1249  [2]
 LDCM0371  CL102 PaTu 8988t C135(1.27)  LDD1250  [2]
 LDCM0372  CL103 PaTu 8988t C135(0.95)  LDD1251  [2]
 LDCM0373  CL104 PaTu 8988t C135(0.89)  LDD1252  [2]
 LDCM0374  CL105 PaTu 8988t C141(0.85)  LDD1253  [2]
 LDCM0375  CL106 PaTu 8988t C141(0.84)  LDD1254  [2]
 LDCM0376  CL107 PaTu 8988t C141(0.75)  LDD1255  [2]
 LDCM0377  CL108 PaTu 8988t C141(0.83)  LDD1256  [2]
 LDCM0378  CL109 PaTu 8988t C141(1.01)  LDD1257  [2]
 LDCM0379  CL11 HEK-293T C81(0.99)  LDD1583  [5]
 LDCM0380  CL110 PaTu 8988t C141(1.04)  LDD1259  [2]
 LDCM0381  CL111 PaTu 8988t C141(1.08)  LDD1260  [2]
 LDCM0382  CL112 HCT 116 C609(0.85)  LDD0699  [2]
 LDCM0383  CL113 HCT 116 C609(1.15)  LDD0700  [2]
 LDCM0384  CL114 HCT 116 C609(0.89)  LDD0701  [2]
 LDCM0385  CL115 HCT 116 C609(1.41)  LDD0702  [2]
 LDCM0386  CL116 HCT 116 C609(1.08)  LDD0703  [2]
 LDCM0387  CL117 HCT 116 C609(0.84)  LDD0704  [2]
 LDCM0388  CL118 HCT 116 C609(0.82)  LDD0705  [2]
 LDCM0389  CL119 HCT 116 C609(0.85)  LDD0706  [2]
 LDCM0390  CL12 HEK-293T C135(1.05)  LDD1594  [5]
 LDCM0391  CL120 HCT 116 C609(0.89)  LDD0708  [2]
 LDCM0392  CL121 HCT 116 C609(0.59)  LDD0709  [2]
 LDCM0393  CL122 HCT 116 C609(0.92)  LDD0710  [2]
 LDCM0394  CL123 HCT 116 C609(1.15)  LDD0711  [2]
 LDCM0395  CL124 HCT 116 C609(0.75)  LDD0712  [2]
 LDCM0396  CL125 HCT 116 C141(0.91)  LDD0713  [2]
 LDCM0397  CL126 HCT 116 C141(1.04)  LDD0714  [2]
 LDCM0398  CL127 HCT 116 C141(1.17)  LDD0715  [2]
 LDCM0399  CL128 HCT 116 C141(0.98)  LDD0716  [2]
 LDCM0401  CL14 HEK-293T C135(1.09)  LDD1605  [5]
 LDCM0402  CL15 HEK-293T C135(0.90)  LDD1606  [5]
 LDCM0403  CL16 HEK-293T C135(1.06)  LDD1607  [5]
 LDCM0405  CL18 HEK-293T C135(0.93); C81(1.08)  LDD1609  [5]
 LDCM0407  CL2 HEK-293T C135(1.06)  LDD1611  [5]
 LDCM0411  CL23 HEK-293T C81(1.25)  LDD1615  [5]
 LDCM0412  CL24 HEK-293T C135(1.12)  LDD1616  [5]
 LDCM0414  CL26 HEK-293T C135(1.06)  LDD1618  [5]
 LDCM0415  CL27 HEK-293T C135(0.87)  LDD1619  [5]
 LDCM0416  CL28 HEK-293T C135(1.14)  LDD1620  [5]
 LDCM0418  CL3 HEK-293T C135(1.04)  LDD1622  [5]
 LDCM0419  CL30 HEK-293T C135(1.07); C81(1.20)  LDD1623  [5]
 LDCM0420  CL31 HCT 116 C141(0.94); C609(1.42)  LDD0737  [2]
 LDCM0421  CL32 HCT 116 C141(1.39); C609(0.77)  LDD0738  [2]
 LDCM0422  CL33 HCT 116 C141(1.34); C609(1.25)  LDD0739  [2]
 LDCM0423  CL34 HCT 116 C141(1.07); C609(1.22)  LDD0740  [2]
 LDCM0424  CL35 HCT 116 C141(1.12); C609(1.46)  LDD0741  [2]
 LDCM0425  CL36 HCT 116 C141(0.97); C609(1.04)  LDD0742  [2]
 LDCM0426  CL37 HCT 116 C141(0.97); C609(1.35)  LDD0743  [2]
 LDCM0428  CL39 HCT 116 C141(1.24); C609(1.20)  LDD0745  [2]
 LDCM0429  CL4 HEK-293T C135(1.14)  LDD1633  [5]
 LDCM0430  CL40 HCT 116 C141(0.87); C609(1.07)  LDD0747  [2]
 LDCM0431  CL41 HCT 116 C141(1.02); C609(1.09)  LDD0748  [2]
 LDCM0432  CL42 HCT 116 C141(0.82); C609(1.09)  LDD0749  [2]
 LDCM0433  CL43 HCT 116 C141(1.34); C609(1.06)  LDD0750  [2]
 LDCM0434  CL44 HCT 116 C141(1.03); C609(1.12)  LDD0751  [2]
 LDCM0435  CL45 HCT 116 C141(1.03); C609(1.39)  LDD0752  [2]
 LDCM0437  CL47 HEK-293T C81(1.16)  LDD1641  [5]
 LDCM0438  CL48 HEK-293T C135(0.99)  LDD1642  [5]
 LDCM0441  CL50 HEK-293T C135(0.94)  LDD1645  [5]
 LDCM0443  CL52 HEK-293T C135(1.21)  LDD1646  [5]
 LDCM0445  CL54 HEK-293T C135(1.03); C81(0.95)  LDD1648  [5]
 LDCM0450  CL59 HEK-293T C81(1.22)  LDD1653  [5]
 LDCM0451  CL6 HEK-293T C135(1.15); C81(1.01)  LDD1654  [5]
 LDCM0452  CL60 HEK-293T C135(1.03)  LDD1655  [5]
 LDCM0454  CL62 HEK-293T C135(0.90)  LDD1657  [5]
 LDCM0455  CL63 HEK-293T C135(1.16)  LDD1658  [5]
 LDCM0456  CL64 HEK-293T C135(1.16)  LDD1659  [5]
 LDCM0458  CL66 HEK-293T C135(0.99); C81(1.25)  LDD1661  [5]
 LDCM0464  CL71 HEK-293T C81(1.26)  LDD1667  [5]
 LDCM0465  CL72 HEK-293T C135(1.09)  LDD1668  [5]
 LDCM0467  CL74 HEK-293T C135(1.09)  LDD1670  [5]
 LDCM0469  CL76 HEK-293T C135(0.93)  LDD1672  [5]
 LDCM0471  CL78 HEK-293T C135(1.21); C81(1.26)  LDD1674  [5]
 LDCM0477  CL83 HEK-293T C81(1.06)  LDD1680  [5]
 LDCM0478  CL84 HEK-293T C135(1.05)  LDD1681  [5]
 LDCM0480  CL86 HEK-293T C135(0.96)  LDD1683  [5]
 LDCM0481  CL87 HEK-293T C135(0.97)  LDD1684  [5]
 LDCM0482  CL88 HEK-293T C135(1.00)  LDD1685  [5]
 LDCM0485  CL90 HEK-293T C135(1.19); C81(1.09)  LDD1688  [5]
 LDCM0486  CL91 HCT 116 C609(1.02)  LDD0803  [2]
 LDCM0487  CL92 HCT 116 C609(1.01)  LDD0804  [2]
 LDCM0488  CL93 HCT 116 C609(0.99)  LDD0805  [2]
 LDCM0489  CL94 HCT 116 C609(1.00)  LDD0806  [2]
 LDCM0490  CL95 HCT 116 C609(0.89)  LDD0807  [2]
 LDCM0491  CL96 HCT 116 C609(0.93)  LDD0808  [2]
 LDCM0492  CL97 HCT 116 C609(0.94)  LDD0809  [2]
 LDCM0493  CL98 HCT 116 C609(0.99)  LDD0810  [2]
 LDCM0494  CL99 HCT 116 C609(1.07)  LDD0811  [2]
 LDCM0495  E2913 HEK-293T C135(0.91)  LDD1698  [5]
 LDCM0468  Fragment33 HEK-293T C135(0.95)  LDD1671  [5]
 LDCM0427  Fragment51 HCT 116 C141(0.70); C609(1.12)  LDD0744  [2]
 LDCM0022  KB02 HEK-293T C141(1.00)  LDD1492  [5]
 LDCM0023  KB03 HEK-293T C141(1.02)  LDD1497  [5]
 LDCM0024  KB05 COLO792 C135(1.53)  LDD3310  [6]

References

1 Oxidant-Induced Bioconjugation for Protein Labeling in Live Cells. ACS Chem Biol. 2023 Jan 20;18(1):112-122. doi: 10.1021/acschembio.2c00740. Epub 2022 Dec 21.
2 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
3 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
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Mass spectrometry data entry: PXD044402
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Mass spectrometry data entry: PXD047840