Details of the Target

General Information of Target

Target ID LDTP08984
Target Name Protein enabled homolog (ENAH)
Gene Name ENAH
Gene ID 55740
Synonyms
MENA; Protein enabled homolog
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MSEQSICQARAAVMVYDDANKKWVPAGGSTGFSRVHIYHHTGNNTFRVVGRKIQDHQVVI
NCAIPKGLKYNQATQTFHQWRDARQVYGLNFGSKEDANVFASAMMHALEVLNSQETGPTL
PRQNSQLPAQVQNGPSQEELEIQRRQLQEQQRQKELERERLERERMERERLERERLERER
LERERLEQEQLERERQERERQERLERQERLERQERLERQERLDRERQERQERERLERLER
ERQERERQEQLEREQLEWERERRISSAAAPASVETPLNSVLGDSSASEPGLQAASQPAET
PSQQGIVLGPLAPPPPPPLPPGPAQASVALPPPPGPPPPPPLPSTGPPPPPPPPPLPNQV
PPPPPPPPAPPLPASGFFLASMSEDNRPLTGLAAAIAGAKLRKVSRMEDTSFPSGGNAIG
VNSASSKTDTGRGNGPLPLGGSGLMEEMSALLARRRRIAEKGSTIETEQKEDKGEDSEPV
TSKASSTSTPEPTRKPWERTNTMNGSKSPVISRRDSPRKNQIVFDNRSYDSLHRPKSTPL
SQPSANGVQTEGLDYDRLKQDILDEMRKELTKLKEELIDAIRQELSKSNTA
Target Type
Literature-reported
Target Bioclass
Other
Family
Ena/VASP family
Subcellular location
Cytoplasm
Function
Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance and lamellipodial and filopodial dynamics in migrating cells. ENAH induces the formation of F-actin rich outgrowths in fibroblasts. Acts synergistically with BAIAP2-alpha and downstream of NTN1 to promote filipodia formation.
TTD ID
T95238
Uniprot ID
Q8N8S7
DrugMap ID
TTY36UA
Ensemble ID
ENST00000366843.7
HGNC ID
HGNC:18271

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 22 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
OPA-S-S-alkyne
 Probe Info  		K559(2.92); K574(5.16)  LDD3494  [1]
Probe 1
 Probe Info  		Y16(43.38); Y87(8.59); Y555(9.75)  LDD3495  [2]
BTD
 Probe Info  		C62(1.95)  LDD1700  [3]
HHS-475
 Probe Info  		Y87(0.96); Y38(1.20); Y16(1.27)  LDD0264  [4]
HHS-465
 Probe Info  		Y16(10.00); Y38(7.45); Y529(10.00); Y70(5.83)  LDD2237  [5]
DBIA
 Probe Info  		C62(0.76)  LDD0531  [6]
5E-2FA
 Probe Info  		H56(0.00); H39(0.00); H78(0.00)  LDD2235  [7]
ATP probe
 Probe Info  		N.A.  LDD0199  [8]
m-APA
 Probe Info  		H106(0.00); H56(0.00)  LDD2231  [7]
IA-alkyne
 Probe Info  		C62(0.00); C7(0.00)  LDD0165  [9]
Lodoacetamide azide
 Probe Info  		N.A.  LDD0037  [10]
JW-RF-010
 Probe Info  		C62(0.00); C7(0.00)  LDD0026  [11]
IPM
 Probe Info  		N.A.  LDD0005  [12]
NHS
 Probe Info  		K574(0.00); K559(0.00)  LDD0010  [12]
SF
 Probe Info  		N.A.  LDD0028  [13]
STPyne
 Probe Info  		K574(0.00); K559(0.00)  LDD0009  [12]
YN-1
 Probe Info  		N.A.  LDD0446  [14]
Ox-W18
 Probe Info  		W23(0.00); W258(0.00)  LDD2175  [15]
1c-yne
 Probe Info  		K427(0.00); K94(0.00)  LDD0228  [16]
1d-yne
 Probe Info  		K427(0.00); K461(0.00)  LDD0357  [16]
Acrolein
 Probe Info  		N.A.  LDD0217  [17]
NAIA_5
 Probe Info  		C62(0.00); C7(0.00)  LDD2223  [18]
PAL-AfBPP Probe
Click To Hide/Show 3 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C187
 Probe Info  		18.51  LDD1865  [19]
C191
 Probe Info  		10.85  LDD1868  [19]
C193
 Probe Info  		5.66  LDD1869  [19]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0548  1-(4-(Benzo[d][1,3]dioxol-5-ylmethyl)piperazin-1-yl)-2-nitroethan-1-one MDA-MB-231 C62(0.40)  LDD2142  [3]
 LDCM0524  2-Cyano-N-(2-morpholin-4-yl-ethyl)-acetamide MDA-MB-231 C62(0.95)  LDD2117  [3]
 LDCM0558  2-Cyano-N-phenylacetamide MDA-MB-231 C62(1.12)  LDD2152  [3]
 LDCM0214  AC1 HCT 116 C62(0.76)  LDD0531  [6]
 LDCM0279  AC2 HCT 116 C62(0.94)  LDD0596  [6]
 LDCM0290  AC3 HCT 116 C62(0.96)  LDD0607  [6]
 LDCM0301  AC4 HCT 116 C62(0.97)  LDD0618  [6]
 LDCM0312  AC5 HCT 116 C62(0.98)  LDD0629  [6]
 LDCM0108  Chloroacetamide HeLa N.A.  LDD0222  [17]
 LDCM0367  CL1 HCT 116 C62(0.99)  LDD0684  [6]
 LDCM0368  CL10 HCT 116 C62(0.69)  LDD0685  [6]
 LDCM0369  CL100 HCT 116 C62(0.91)  LDD0686  [6]
 LDCM0379  CL11 HCT 116 C62(0.85)  LDD0696  [6]
 LDCM0390  CL12 HCT 116 C62(1.06)  LDD0707  [6]
 LDCM0400  CL13 HCT 116 C62(0.78)  LDD0717  [6]
 LDCM0401  CL14 HCT 116 C62(0.87)  LDD0718  [6]
 LDCM0402  CL15 HCT 116 C62(0.52)  LDD0719  [6]
 LDCM0407  CL2 HCT 116 C62(0.93)  LDD0724  [6]
 LDCM0418  CL3 HCT 116 C62(0.88)  LDD0735  [6]
 LDCM0420  CL31 HCT 116 C62(1.12)  LDD0737  [6]
 LDCM0421  CL32 HCT 116 C62(1.15)  LDD0738  [6]
 LDCM0422  CL33 HCT 116 C62(0.97)  LDD0739  [6]
 LDCM0423  CL34 HCT 116 C62(1.07)  LDD0740  [6]
 LDCM0424  CL35 HCT 116 C62(1.00)  LDD0741  [6]
 LDCM0425  CL36 HCT 116 C62(0.92)  LDD0742  [6]
 LDCM0426  CL37 HCT 116 C62(1.26)  LDD0743  [6]
 LDCM0428  CL39 HCT 116 C62(1.18)  LDD0745  [6]
 LDCM0429  CL4 HCT 116 C62(0.85)  LDD0746  [6]
 LDCM0430  CL40 HCT 116 C62(1.05)  LDD0747  [6]
 LDCM0431  CL41 HCT 116 C62(1.20)  LDD0748  [6]
 LDCM0432  CL42 HCT 116 C62(0.96)  LDD0749  [6]
 LDCM0433  CL43 HCT 116 C62(1.06)  LDD0750  [6]
 LDCM0434  CL44 HCT 116 C62(1.20)  LDD0751  [6]
 LDCM0435  CL45 HCT 116 C62(0.98)  LDD0752  [6]
 LDCM0440  CL5 HCT 116 C62(0.93)  LDD0757  [6]
 LDCM0451  CL6 HCT 116 C62(0.70)  LDD0768  [6]
 LDCM0453  CL61 HCT 116 C62(0.94)  LDD0770  [6]
 LDCM0454  CL62 HCT 116 C62(0.96)  LDD0771  [6]
 LDCM0455  CL63 HCT 116 C62(0.88)  LDD0772  [6]
 LDCM0456  CL64 HCT 116 C62(0.89)  LDD0773  [6]
 LDCM0457  CL65 HCT 116 C62(0.96)  LDD0774  [6]
 LDCM0458  CL66 HCT 116 C62(1.00)  LDD0775  [6]
 LDCM0459  CL67 HCT 116 C62(0.91)  LDD0776  [6]
 LDCM0460  CL68 HCT 116 C62(0.75)  LDD0777  [6]
 LDCM0461  CL69 HCT 116 C62(0.75)  LDD0778  [6]
 LDCM0462  CL7 HCT 116 C62(0.87)  LDD0779  [6]
 LDCM0463  CL70 HCT 116 C62(0.74)  LDD0780  [6]
 LDCM0464  CL71 HCT 116 C62(0.85)  LDD0781  [6]
 LDCM0465  CL72 HCT 116 C62(1.06)  LDD0782  [6]
 LDCM0466  CL73 HCT 116 C62(0.88)  LDD0783  [6]
 LDCM0467  CL74 HCT 116 C62(0.89)  LDD0784  [6]
 LDCM0473  CL8 HCT 116 C62(0.94)  LDD0790  [6]
 LDCM0484  CL9 HCT 116 C62(0.89)  LDD0801  [6]
 LDCM0486  CL91 HCT 116 C62(0.95)  LDD0803  [6]
 LDCM0487  CL92 HCT 116 C62(0.87)  LDD0804  [6]
 LDCM0488  CL93 HCT 116 C62(0.92)  LDD0805  [6]
 LDCM0489  CL94 HCT 116 C62(0.92)  LDD0806  [6]
 LDCM0490  CL95 HCT 116 C62(1.04)  LDD0807  [6]
 LDCM0491  CL96 HCT 116 C62(0.89)  LDD0808  [6]
 LDCM0492  CL97 HCT 116 C62(0.91)  LDD0809  [6]
 LDCM0493  CL98 HCT 116 C62(0.97)  LDD0810  [6]
 LDCM0494  CL99 HCT 116 C62(0.93)  LDD0811  [6]
 LDCM0468  Fragment33 HCT 116 C62(0.93)  LDD0785  [6]
 LDCM0427  Fragment51 HCT 116 C62(0.51)  LDD0744  [6]
 LDCM0116  HHS-0101 DM93 Y87(0.96); Y38(1.20); Y16(1.27)  LDD0264  [4]
 LDCM0117  HHS-0201 DM93 Y38(0.74); Y87(0.92); Y16(1.01)  LDD0265  [4]
 LDCM0118  HHS-0301 DM93 Y38(0.40); Y87(0.86); Y16(0.93)  LDD0266  [4]
 LDCM0119  HHS-0401 DM93 Y38(0.49); Y87(1.13); Y16(1.30)  LDD0267  [4]
 LDCM0120  HHS-0701 DM93 Y38(0.71); Y16(0.75); Y87(0.83)  LDD0268  [4]
 LDCM0022  KB02 HCC1954 C62(1.30)  LDD2343  [20]
 LDCM0023  KB03 MDA-MB-231 C62(0.80)  LDD1701  [3]
 LDCM0024  KB05 HCC1954 C62(1.48)  LDD3177  [20]
 LDCM0500  Nucleophilic fragment 13a MDA-MB-231 C62(1.06)  LDD2093  [3]
 LDCM0505  Nucleophilic fragment 15b MDA-MB-231 C62(0.82)  LDD2098  [3]
 LDCM0506  Nucleophilic fragment 16a MDA-MB-231 C62(1.13)  LDD2099  [3]
 LDCM0508  Nucleophilic fragment 17a MDA-MB-231 C62(0.88)  LDD2101  [3]
 LDCM0511  Nucleophilic fragment 18b MDA-MB-231 C62(0.39)  LDD2104  [3]
 LDCM0513  Nucleophilic fragment 19b MDA-MB-231 C62(0.52)  LDD2106  [3]
 LDCM0514  Nucleophilic fragment 20a MDA-MB-231 C62(1.23)  LDD2107  [3]
 LDCM0515  Nucleophilic fragment 20b MDA-MB-231 C62(0.60)  LDD2108  [3]
 LDCM0516  Nucleophilic fragment 21a MDA-MB-231 C62(1.29)  LDD2109  [3]
 LDCM0517  Nucleophilic fragment 21b MDA-MB-231 C62(0.42)  LDD2110  [3]
 LDCM0518  Nucleophilic fragment 22a MDA-MB-231 C62(1.10)  LDD2111  [3]
 LDCM0522  Nucleophilic fragment 24a MDA-MB-231 C62(0.45)  LDD2115  [3]
 LDCM0526  Nucleophilic fragment 26a MDA-MB-231 C62(2.10)  LDD2119  [3]
 LDCM0532  Nucleophilic fragment 29a MDA-MB-231 C62(0.87)  LDD2125  [3]
 LDCM0534  Nucleophilic fragment 30a MDA-MB-231 C62(1.05)  LDD2127  [3]
 LDCM0536  Nucleophilic fragment 31 MDA-MB-231 C62(1.07)  LDD2129  [3]
 LDCM0541  Nucleophilic fragment 36 MDA-MB-231 C62(0.50)  LDD2134  [3]
 LDCM0542  Nucleophilic fragment 37 MDA-MB-231 C62(1.37)  LDD2135  [3]
 LDCM0544  Nucleophilic fragment 39 MDA-MB-231 C62(0.98)  LDD2137  [3]
 LDCM0211  Nucleophilic fragment 3b MDA-MB-231 C62(1.95)  LDD1700  [3]
 LDCM0546  Nucleophilic fragment 40 MDA-MB-231 C62(0.76)  LDD2140  [3]
 LDCM0550  Nucleophilic fragment 5a MDA-MB-231 C62(2.70)  LDD2144  [3]
 LDCM0552  Nucleophilic fragment 6a MDA-MB-231 C62(0.96)  LDD2146  [3]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Transcription factor
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Telomeric repeat-binding factor 2-interacting protein 1 (TERF2IP) RAP1 family Q9NYB0
Other
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Testin (TES) Prickle / espinas / testin family Q9UGI8
Protocadherin Fat 1 (FAT1) . Q14517

References

1 A chemical proteomics approach for global mapping of functional lysines on cell surface of living cell. Nat Commun. 2024 Apr 8;15(1):2997. doi: 10.1038/s41467-024-47033-w.
Mass spectrometry data entry: PXD042888
2 An Azo Coupling-Based Chemoproteomic Approach to Systematically Profile the Tyrosine Reactivity in the Human Proteome. Anal Chem. 2021 Jul 27;93(29):10334-10342. doi: 10.1021/acs.analchem.1c01935. Epub 2021 Jul 12.
3 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
4 Discovery of a Cell-Active SuTEx Ligand of Prostaglandin Reductase 2. Chembiochem. 2021 Jun 15;22(12):2134-2139. doi: 10.1002/cbic.202000879. Epub 2021 Apr 29.
5 Global targeting of functional tyrosines using sulfur-triazole exchange chemistry. Nat Chem Biol. 2020 Feb;16(2):150-159. doi: 10.1038/s41589-019-0404-5. Epub 2019 Nov 25.
6 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
7 Global profiling of functional histidines in live cells using small-molecule photosensitizer and chemical probe relay labelling. Nat Chem. 2024 Jun 4. doi: 10.1038/s41557-024-01545-6. Online ahead of print.
Mass spectrometry data entry: PXD042377
8 Targeted Proteomic Approaches for Proteome-Wide Characterizations of the AMP-Binding Capacities of Kinases. J Proteome Res. 2022 Aug 5;21(8):2063-2070. doi: 10.1021/acs.jproteome.2c00225. Epub 2022 Jul 12.
9 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
10 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
11 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
12 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
13 Solid Phase Synthesis of Fluorosulfate Containing Macrocycles for Chemoproteomic Workflows. bioRxiv [Preprint]. 2023 Feb 18:2023.02.17.529022. doi: 10.1101/2023.02.17.529022.
Mass spectrometry data entry: PXD039931
14 Ynamide Electrophile for the Profiling of Ligandable Carboxyl Residues in Live Cells and the Development of New Covalent Inhibitors. J Med Chem. 2022 Aug 11;65(15):10408-10418. doi: 10.1021/acs.jmedchem.2c00272. Epub 2022 Jul 26.
15 Oxidative cyclization reagents reveal tryptophan cation- interactions. Nature. 2024 Mar;627(8004):680-687. doi: 10.1038/s41586-024-07140-6. Epub 2024 Mar 6.
Mass spectrometry data entry: PXD001377 , PXD005252
16 Tunable Amine-Reactive Electrophiles for Selective Profiling of Lysine. Angew Chem Int Ed Engl. 2022 Jan 26;61(5):e202112107. doi: 10.1002/anie.202112107. Epub 2021 Dec 16.
17 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
18 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
19 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587
20 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840