Details of the Target

General Information of Target

Target ID LDTP08409
Target Name Transcriptional repressor p66-alpha (GATAD2A)
Gene Name GATAD2A
Gene ID 54815
Synonyms
Transcriptional repressor p66-alpha; Hp66alpha; GATA zinc finger domain-containing protein 2A
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MTEEACRTRSQKRALERDPTEDDVESKKIKMERGLLASDLNTDGDMRVTPEPGAGPTQGL
LRATEATAMAMGRGEGLVGDGPVDMRTSHSDMKSERRPPSPDVIVLSDNEQPSSPRVNGL
TTVALKETSTEALMKSSPEERERMIKQLKEELRLEEAKLVLLKKLRQSQIQKEATAQKPT
GSVGSTVTTPPPLVRGTQNIPAGKPSLQTSSARMPGSVIPPPLVRGGQQASSKLGPQASS
QVVMPPLVRGAQQIHSIRQHSSTGPPPLLLAPRASVPSVQIQGQRIIQQGLIRVANVPNT
SLLVNIPQPTPASLKGTTATSAQANSTPTSVASVVTSAESPASRQAAAKLALRKQLEKTL
LEIPPPKPPAPEMNFLPSAANNEFIYLVGLEEVVQNLLETQGRMSAATVLSREPYMCAQC
KTDFTCRWREEKSGAIMCENCMTTNQKKALKVEHTSRLKAAFVKALQQEQEIEQRLLQQG
TAPAQAKAEPTAAPHPVLKQVIKPRRKLAFRSGEARDWSNGAVLQASSQLSRGSATTPRG
VLHTFSPSPKLQNSASATALVSRTGRHSERTVSAGKGSATSNWKKTPLSTGGTLAFVSPS
LAVHKSSSAVDRQREYLLDMIPPRSIPQSATWK
Target Bioclass
Transcription factor
Subcellular location
Nucleus speckle
Function
Transcriptional repressor. Acts as a component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin. Enhances MBD2-mediated repression. Efficient repression requires the presence of GATAD2B.
Uniprot ID
Q86YP4
Ensemble ID
ENST00000358713.7
HGNC ID
HGNC:29989

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 16 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
m-APA
 Probe Info  		15.00  LDD0402  [1]
STPyne
 Probe Info  		K459(8.58)  LDD0277  [2]
DBIA
 Probe Info  		C427(3.24)  LDD3337  [3]
BTD
 Probe Info  		C426(1.09)  LDD2107  [4]
IA-alkyne
 Probe Info  		N.A.  LDD0162  [5]
NAIA_5
 Probe Info  		N.A.  LDD2224  [6]
Compound 10
 Probe Info  		N.A.  LDD2216  [7]
ENE
 Probe Info  		N.A.  LDD0006  [8]
IPM
 Probe Info  		N.A.  LDD0005  [8]
TFBX
 Probe Info  		N.A.  LDD0148  [9]
VSF
 Probe Info  		N.A.  LDD0007  [8]
Phosphinate-6
 Probe Info  		N.A.  LDD0018  [10]
Acrolein
 Probe Info  		N.A.  LDD0217  [11]
Crotonaldehyde
 Probe Info  		N.A.  LDD0219  [11]
Methacrolein
 Probe Info  		N.A.  LDD0218  [11]
AOyne
 Probe Info  		6.10  LDD0443  [12]
PAL-AfBPP Probe
Click To Hide/Show 3 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C187
 Probe Info  		21.86  LDD1865  [13]
C191
 Probe Info  		14.12  LDD1868  [13]
C193
 Probe Info  		8.34  LDD1869  [13]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0226  AC11 HEK-293T C420(1.00)  LDD1509  [14]
 LDCM0237  AC12 HEK-293T C441(0.92)  LDD1510  [14]
 LDCM0259  AC14 HEK-293T C417(0.94); C441(0.98)  LDD1512  [14]
 LDCM0278  AC19 HEK-293T C420(0.66)  LDD1517  [14]
 LDCM0280  AC20 HEK-293T C441(0.93)  LDD1519  [14]
 LDCM0281  AC21 HEK-293T C420(1.03)  LDD1520  [14]
 LDCM0282  AC22 HEK-293T C417(0.91); C441(1.04)  LDD1521  [14]
 LDCM0284  AC24 HEK-293T C420(0.95)  LDD1523  [14]
 LDCM0287  AC27 HEK-293T C420(1.02)  LDD1526  [14]
 LDCM0288  AC28 HEK-293T C441(1.01)  LDD1527  [14]
 LDCM0289  AC29 HEK-293T C420(1.22)  LDD1528  [14]
 LDCM0290  AC3 HEK-293T C420(1.10)  LDD1529  [14]
 LDCM0291  AC30 HEK-293T C417(0.97); C441(1.10)  LDD1530  [14]
 LDCM0293  AC32 HEK-293T C420(1.26)  LDD1532  [14]
 LDCM0296  AC35 HEK-293T C420(1.17)  LDD1535  [14]
 LDCM0297  AC36 HEK-293T C441(1.02)  LDD1536  [14]
 LDCM0298  AC37 HEK-293T C420(1.13)  LDD1537  [14]
 LDCM0299  AC38 HEK-293T C417(1.01); C441(0.99)  LDD1538  [14]
 LDCM0301  AC4 HEK-293T C441(1.04)  LDD1540  [14]
 LDCM0302  AC40 HEK-293T C420(1.00)  LDD1541  [14]
 LDCM0305  AC43 HEK-293T C420(1.03)  LDD1544  [14]
 LDCM0306  AC44 HEK-293T C441(0.86)  LDD1545  [14]
 LDCM0307  AC45 HEK-293T C420(1.00)  LDD1546  [14]
 LDCM0308  AC46 HEK-293T C417(1.02); C441(1.04)  LDD1547  [14]
 LDCM0310  AC48 HEK-293T C420(0.94)  LDD1549  [14]
 LDCM0312  AC5 HEK-293T C420(0.99)  LDD1551  [14]
 LDCM0314  AC51 HEK-293T C420(1.01)  LDD1553  [14]
 LDCM0315  AC52 HEK-293T C441(1.01)  LDD1554  [14]
 LDCM0316  AC53 HEK-293T C420(1.04)  LDD1555  [14]
 LDCM0317  AC54 HEK-293T C417(1.08); C441(1.03)  LDD1556  [14]
 LDCM0319  AC56 HEK-293T C420(1.10)  LDD1558  [14]
 LDCM0322  AC59 HEK-293T C420(0.97)  LDD1561  [14]
 LDCM0323  AC6 HEK-293T C417(1.16); C441(1.12)  LDD1562  [14]
 LDCM0324  AC60 HEK-293T C441(0.92)  LDD1563  [14]
 LDCM0325  AC61 HEK-293T C420(1.19)  LDD1564  [14]
 LDCM0326  AC62 HEK-293T C417(0.90); C441(0.94)  LDD1565  [14]
 LDCM0328  AC64 HEK-293T C420(1.03)  LDD1567  [14]
 LDCM0345  AC8 HEK-293T C420(1.17)  LDD1569  [14]
 LDCM0248  AKOS034007472 HEK-293T C420(1.15)  LDD1511  [14]
 LDCM0275  AKOS034007705 HEK-293T C420(1.09)  LDD1514  [14]
 LDCM0156  Aniline NCI-H1299 12.73  LDD0403  [1]
 LDCM0108  Chloroacetamide HeLa N.A.  LDD0222  [11]
 LDCM0368  CL10 HEK-293T C417(1.12); C441(1.21)  LDD1572  [14]
 LDCM0369  CL100 HEK-293T C441(1.20)  LDD1573  [14]
 LDCM0371  CL102 HEK-293T C420(1.12)  LDD1575  [14]
 LDCM0372  CL103 HEK-293T C441(0.94)  LDD1576  [14]
 LDCM0373  CL104 HEK-293T C441(1.10)  LDD1577  [14]
 LDCM0375  CL106 HEK-293T C420(1.14)  LDD1579  [14]
 LDCM0376  CL107 HEK-293T C441(1.01)  LDD1580  [14]
 LDCM0377  CL108 HEK-293T C441(1.10)  LDD1581  [14]
 LDCM0380  CL110 HEK-293T C420(1.17)  LDD1584  [14]
 LDCM0381  CL111 HEK-293T C441(0.83)  LDD1585  [14]
 LDCM0382  CL112 HEK-293T C441(1.18)  LDD1586  [14]
 LDCM0384  CL114 HEK-293T C420(1.16)  LDD1588  [14]
 LDCM0385  CL115 HEK-293T C441(0.98)  LDD1589  [14]
 LDCM0386  CL116 HEK-293T C441(1.11)  LDD1590  [14]
 LDCM0388  CL118 HEK-293T C420(1.04)  LDD1592  [14]
 LDCM0389  CL119 HEK-293T C441(1.06)  LDD1593  [14]
 LDCM0390  CL12 HEK-293T C420(1.11)  LDD1594  [14]
 LDCM0391  CL120 HEK-293T C441(1.09)  LDD1595  [14]
 LDCM0393  CL122 HEK-293T C420(1.15)  LDD1597  [14]
 LDCM0394  CL123 HEK-293T C441(0.85)  LDD1598  [14]
 LDCM0395  CL124 HEK-293T C441(1.02)  LDD1599  [14]
 LDCM0397  CL126 HEK-293T C420(1.18)  LDD1601  [14]
 LDCM0398  CL127 HEK-293T C441(1.31)  LDD1602  [14]
 LDCM0399  CL128 HEK-293T C441(1.20)  LDD1603  [14]
 LDCM0401  CL14 HEK-293T C420(1.15)  LDD1605  [14]
 LDCM0402  CL15 HEK-293T C441(0.87)  LDD1606  [14]
 LDCM0403  CL16 HEK-293T C441(1.02)  LDD1607  [14]
 LDCM0406  CL19 HEK-293T C420(1.09)  LDD1610  [14]
 LDCM0407  CL2 HEK-293T C420(1.06)  LDD1611  [14]
 LDCM0408  CL20 HEK-293T C441(0.81)  LDD1612  [14]
 LDCM0409  CL21 HEK-293T C420(1.06)  LDD1613  [14]
 LDCM0410  CL22 HEK-293T C417(1.09); C441(0.99)  LDD1614  [14]
 LDCM0412  CL24 HEK-293T C420(1.12)  LDD1616  [14]
 LDCM0414  CL26 HEK-293T C420(1.06)  LDD1618  [14]
 LDCM0415  CL27 HEK-293T C441(0.79)  LDD1619  [14]
 LDCM0416  CL28 HEK-293T C441(0.99)  LDD1620  [14]
 LDCM0418  CL3 HEK-293T C441(1.11)  LDD1622  [14]
 LDCM0420  CL31 HEK-293T C420(1.04)  LDD1624  [14]
 LDCM0421  CL32 HEK-293T C441(0.88)  LDD1625  [14]
 LDCM0422  CL33 HEK-293T C420(1.05)  LDD1626  [14]
 LDCM0423  CL34 HEK-293T C417(1.03); C441(0.77)  LDD1627  [14]
 LDCM0425  CL36 HEK-293T C420(1.10)  LDD1629  [14]
 LDCM0428  CL39 HEK-293T C441(1.03)  LDD1632  [14]
 LDCM0429  CL4 HEK-293T C441(1.11)  LDD1633  [14]
 LDCM0430  CL40 HEK-293T C441(1.07)  LDD1634  [14]
 LDCM0433  CL43 HEK-293T C420(0.91)  LDD1637  [14]
 LDCM0434  CL44 HEK-293T C441(0.95)  LDD1638  [14]
 LDCM0435  CL45 HEK-293T C420(1.02)  LDD1639  [14]
 LDCM0436  CL46 HEK-293T C417(1.24); C441(0.92)  LDD1640  [14]
 LDCM0438  CL48 HEK-293T C420(1.13)  LDD1642  [14]
 LDCM0441  CL50 HEK-293T C420(1.08)  LDD1645  [14]
 LDCM0443  CL52 HEK-293T C441(1.11)  LDD1646  [14]
 LDCM0446  CL55 HEK-293T C420(1.00)  LDD1649  [14]
 LDCM0447  CL56 HEK-293T C441(0.95)  LDD1650  [14]
 LDCM0448  CL57 HEK-293T C420(1.11)  LDD1651  [14]
 LDCM0449  CL58 HEK-293T C417(1.08); C441(0.94)  LDD1652  [14]
 LDCM0452  CL60 HEK-293T C420(0.93)  LDD1655  [14]
 LDCM0454  CL62 HEK-293T C420(1.01)  LDD1657  [14]
 LDCM0455  CL63 HEK-293T C441(0.81)  LDD1658  [14]
 LDCM0456  CL64 HEK-293T C441(1.08)  LDD1659  [14]
 LDCM0459  CL67 HEK-293T C420(1.05)  LDD1662  [14]
 LDCM0460  CL68 HEK-293T C441(0.72)  LDD1663  [14]
 LDCM0461  CL69 HEK-293T C420(1.33)  LDD1664  [14]
 LDCM0462  CL7 HEK-293T C420(0.98)  LDD1665  [14]
 LDCM0463  CL70 HEK-293T C417(1.13); C441(0.90)  LDD1666  [14]
 LDCM0465  CL72 HEK-293T C420(1.13)  LDD1668  [14]
 LDCM0467  CL74 HEK-293T C420(1.13)  LDD1670  [14]
 LDCM0469  CL76 HEK-293T C441(0.99)  LDD1672  [14]
 LDCM0472  CL79 HEK-293T C420(0.96)  LDD1675  [14]
 LDCM0473  CL8 HEK-293T C441(0.76)  LDD1676  [14]
 LDCM0474  CL80 HEK-293T C441(0.95)  LDD1677  [14]
 LDCM0475  CL81 HEK-293T C420(1.25)  LDD1678  [14]
 LDCM0476  CL82 HEK-293T C417(0.97); C441(1.16)  LDD1679  [14]
 LDCM0478  CL84 HEK-293T C420(0.88)  LDD1681  [14]
 LDCM0480  CL86 HEK-293T C420(1.12)  LDD1683  [14]
 LDCM0481  CL87 HEK-293T C441(1.18)  LDD1684  [14]
 LDCM0482  CL88 HEK-293T C441(1.19)  LDD1685  [14]
 LDCM0484  CL9 HEK-293T C420(1.20)  LDD1687  [14]
 LDCM0486  CL91 HEK-293T C420(1.10)  LDD1689  [14]
 LDCM0487  CL92 HEK-293T C441(0.88)  LDD1690  [14]
 LDCM0488  CL93 HEK-293T C420(1.06)  LDD1691  [14]
 LDCM0489  CL94 HEK-293T C417(1.12); C441(0.82)  LDD1692  [14]
 LDCM0491  CL96 HEK-293T C420(1.10)  LDD1694  [14]
 LDCM0493  CL98 HEK-293T C420(1.05)  LDD1696  [14]
 LDCM0494  CL99 HEK-293T C441(1.01)  LDD1697  [14]
 LDCM0495  E2913 HEK-293T C441(0.79)  LDD1698  [14]
 LDCM0213  Electrophilic fragment 2 MDA-MB-231 C426(0.82)  LDD1702  [4]
 LDCM0468  Fragment33 HEK-293T C441(1.03)  LDD1671  [14]
 LDCM0427  Fragment51 HEK-293T C420(1.05)  LDD1631  [14]
 LDCM0022  KB02 HEK-293T C417(1.15); C420(1.15)  LDD1492  [14]
 LDCM0023  KB03 HEK-293T C417(1.08); C420(1.08)  LDD1497  [14]
 LDCM0024  KB05 MSTO-211H C427(3.24)  LDD3337  [3]
 LDCM0109  NEM HeLa N.A.  LDD0223  [11]
 LDCM0514  Nucleophilic fragment 20a MDA-MB-231 C426(1.09)  LDD2107  [4]
 LDCM0530  Nucleophilic fragment 28a MDA-MB-231 C426(1.04)  LDD2123  [4]
 LDCM0532  Nucleophilic fragment 29a MDA-MB-231 C426(0.94)  LDD2125  [4]
 LDCM0543  Nucleophilic fragment 38 MDA-MB-231 C426(1.59)  LDD2136  [4]
 LDCM0546  Nucleophilic fragment 40 MDA-MB-231 C426(1.14)  LDD2140  [4]
 LDCM0552  Nucleophilic fragment 6a MDA-MB-231 C426(1.26)  LDD2146  [4]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Other
Click To Hide/Show 6 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Golgin subfamily A member 2 (GOLGA2) GOLGA2 family Q08379
Keratin, type I cuticular Ha2 (KRT32) Intermediate filament family Q14532
Keratin, type I cuticular Ha4 (KRT34) Intermediate filament family O76011
Keratin, type I cytoskeletal 40 (KRT40) Intermediate filament family Q6A162
Methyl-CpG-binding domain protein 3-like 1 (MBD3L1) MBD3L family Q8WWY6
Methyl-CpG-binding domain protein 2 (MBD2) . Q9UBB5

References

1 Quantitative and Site-Specific Chemoproteomic Profiling of Targets of Acrolein. Chem Res Toxicol. 2019 Mar 18;32(3):467-473. doi: 10.1021/acs.chemrestox.8b00343. Epub 2019 Jan 15.
2 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
3 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
4 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
5 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
6 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
7 Multiplexed CuAAC Suzuki-Miyaura Labeling for Tandem Activity-Based Chemoproteomic Profiling. Anal Chem. 2021 Feb 2;93(4):2610-2618. doi: 10.1021/acs.analchem.0c04726. Epub 2021 Jan 20.
Mass spectrometry data entry: PXD022279
8 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
9 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
10 DFT-Guided Discovery of Ethynyl-Triazolyl-Phosphinates as Modular Electrophiles for Chemoselective Cysteine Bioconjugation and Profiling. Angew Chem Int Ed Engl. 2022 Oct 10;61(41):e202205348. doi: 10.1002/anie.202205348. Epub 2022 Aug 22.
Mass spectrometry data entry: PXD033004
11 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
12 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
13 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587
14 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402