Details of the Target

General Information of Target

Target ID LDTP07260
Target Name Muscleblind-like protein 2 (MBNL2)
Gene Name MBNL2
Gene ID 10150
Synonyms
MBLL; MBLL39; MLP1; Muscleblind-like protein 2; Muscleblind-like protein 1; Muscleblind-like protein-like; Muscleblind-like protein-like 39
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MALNVAPVRDTKWLTLEVCRQFQRGTCSRSDEECKFAHPPKSCQVENGRVIACFDSLKGR
CSRENCKYLHPPTHLKTQLEINGRNNLIQQKTAAAMLAQQMQFMFPGTPLHPVPTFPVGP
AIGTNTAISFAPYLAPVTPGVGLVPTEILPTTPVIVPGSPPVTVPGSTATQKLLRTDKLE
VCREFQRGNCARGETDCRFAHPADSTMIDTSDNTVTVCMDYIKGRCMREKCKYFHPPAHL
QAKIKAAQHQANQAAVAAQAAAAAATVMAFPPGALHPLPKRQALEKSNGTSAVFNPSVLH
YQQALTSAQLQQHAAFIPTGSVLCMTPATSIDNSEIISRNGMECQESALRITKHCYCTYY
PVSSSIELPQTAC
Target Type
Literature-reported
Target Bioclass
Other
Family
Muscleblind family
Subcellular location
Nucleus
Function
Mediates pre-mRNA alternative splicing regulation. Acts either as activator or repressor of splicing on specific pre-mRNA targets. Inhibits cardiac troponin-T (TNNT2) pre-mRNA exon inclusion but induces insulin receptor (IR) pre-mRNA exon inclusion in muscle. Antagonizes the alternative splicing activity pattern of CELF proteins. RNA-binding protein that binds to 5'ACACCC-3' core sequence, termed zipcode, within the 3'UTR of ITGA3. Binds to CUG triplet repeat expansion in myotonic dystrophy muscle cells by sequestering the target RNAs. Together with RNA binding proteins RBPMS and RBFOX2, activates vascular smooth muscle cells alternative splicing events. Regulates NCOR2 alternative splicing. Seems to regulate expression and localization of ITGA3 by transporting it from the nucleus to cytoplasm at adhesion plaques. May play a role in myotonic dystrophy pathophysiology (DM).
TTD ID
T34708
Uniprot ID
Q5VZF2
DrugMap ID
TTH9OLG
Ensemble ID
ENST00000343600.9
HGNC ID
HGNC:16746

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 15 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
TH211
 Probe Info  		Y233(5.86)  LDD0257  [1]
IPM
 Probe Info  		C53(0.00); C19(0.00); C43(0.00); C182(0.00)  LDD0241  [2]
DBIA
 Probe Info  		C53(0.80)  LDD3310  [3]
BTD
 Probe Info  		C182(1.22)  LDD1700  [4]
HHS-482
 Probe Info  		Y233(1.31)  LDD0285  [5]
HHS-475
 Probe Info  		Y68(0.48)  LDD0264  [6]
HHS-465
 Probe Info  		Y233(5.57)  LDD2237  [7]
IA-alkyne
 Probe Info  		C53(0.00); C19(0.00); C197(0.00); C43(0.00)  LDD0162  [8]
NAIA_4
 Probe Info  		C19(0.00); C53(0.00)  LDD2226  [9]
STPyne
 Probe Info  		N.A.  LDD0009  [10]
TFBX
 Probe Info  		C182(0.00); C43(0.00); C53(0.00)  LDD0148  [11]
Acrolein
 Probe Info  		C19(0.00); C53(0.00); C43(0.00)  LDD0217  [12]
Crotonaldehyde
 Probe Info  		N.A.  LDD0219  [12]
Methacrolein
 Probe Info  		N.A.  LDD0218  [12]
NAIA_5
 Probe Info  		C182(0.00); C43(0.00); C53(0.00); C19(0.00)  LDD2223  [9]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0026  4SU-RNA+native RNA HEK-293T C43(2.45)  LDD0372  [13]
 LDCM0226  AC11 HEK-293T C218(1.03)  LDD1509  [14]
 LDCM0270  AC15 HEK-293T C218(1.08)  LDD1513  [14]
 LDCM0278  AC19 HEK-293T C218(0.83)  LDD1517  [14]
 LDCM0283  AC23 HEK-293T C218(1.03)  LDD1522  [14]
 LDCM0287  AC27 HEK-293T C218(1.00)  LDD1526  [14]
 LDCM0290  AC3 HEK-293T C218(1.03)  LDD1529  [14]
 LDCM0292  AC31 HEK-293T C218(1.11)  LDD1531  [14]
 LDCM0296  AC35 HEK-293T C218(0.94)  LDD1535  [14]
 LDCM0300  AC39 HEK-293T C218(1.18)  LDD1539  [14]
 LDCM0305  AC43 HEK-293T C218(0.93)  LDD1544  [14]
 LDCM0309  AC47 HEK-293T C218(1.24)  LDD1548  [14]
 LDCM0314  AC51 HEK-293T C218(1.20)  LDD1553  [14]
 LDCM0318  AC55 HEK-293T C218(1.15)  LDD1557  [14]
 LDCM0322  AC59 HEK-293T C218(1.13)  LDD1561  [14]
 LDCM0327  AC63 HEK-293T C218(0.96)  LDD1566  [14]
 LDCM0334  AC7 HEK-293T C218(1.28)  LDD1568  [14]
 LDCM0545  Acetamide MDA-MB-231 C182(0.87); C43(0.49)  LDD2138  [4]
 LDCM0108  Chloroacetamide HeLa C19(0.00); C53(0.00)  LDD0222  [12]
 LDCM0367  CL1 HEK-293T C218(1.13)  LDD1571  [14]
 LDCM0369  CL100 HEK-293T C27(0.99); C34(0.99)  LDD1573  [14]
 LDCM0370  CL101 HEK-293T C218(1.01)  LDD1574  [14]
 LDCM0371  CL102 HEK-293T C218(0.86)  LDD1575  [14]
 LDCM0372  CL103 HEK-293T C218(1.22)  LDD1576  [14]
 LDCM0373  CL104 HEK-293T C27(1.06); C34(1.06)  LDD1577  [14]
 LDCM0374  CL105 HEK-293T C218(1.01)  LDD1578  [14]
 LDCM0375  CL106 HEK-293T C218(1.02)  LDD1579  [14]
 LDCM0376  CL107 HEK-293T C218(1.12)  LDD1580  [14]
 LDCM0377  CL108 HEK-293T C27(1.02); C34(1.02)  LDD1581  [14]
 LDCM0378  CL109 HEK-293T C218(0.93)  LDD1582  [14]
 LDCM0379  CL11 HEK-293T C218(1.10)  LDD1583  [14]
 LDCM0380  CL110 HEK-293T C218(0.96)  LDD1584  [14]
 LDCM0381  CL111 HEK-293T C218(1.14)  LDD1585  [14]
 LDCM0382  CL112 HEK-293T C27(1.02); C34(1.02)  LDD1586  [14]
 LDCM0383  CL113 HEK-293T C218(1.32)  LDD1587  [14]
 LDCM0384  CL114 HEK-293T C218(1.02)  LDD1588  [14]
 LDCM0385  CL115 HEK-293T C218(1.18)  LDD1589  [14]
 LDCM0386  CL116 HEK-293T C27(0.99); C34(0.99)  LDD1590  [14]
 LDCM0387  CL117 HEK-293T C218(0.98)  LDD1591  [14]
 LDCM0388  CL118 HEK-293T C218(0.96)  LDD1592  [14]
 LDCM0389  CL119 HEK-293T C218(1.01)  LDD1593  [14]
 LDCM0391  CL120 HEK-293T C27(0.96); C34(0.96)  LDD1595  [14]
 LDCM0392  CL121 HEK-293T C218(1.17)  LDD1596  [14]
 LDCM0393  CL122 HEK-293T C218(0.99)  LDD1597  [14]
 LDCM0394  CL123 HEK-293T C218(1.11)  LDD1598  [14]
 LDCM0395  CL124 HEK-293T C27(0.94); C34(0.94)  LDD1599  [14]
 LDCM0396  CL125 HEK-293T C218(1.14)  LDD1600  [14]
 LDCM0397  CL126 HEK-293T C218(1.03)  LDD1601  [14]
 LDCM0398  CL127 HEK-293T C218(1.03)  LDD1602  [14]
 LDCM0399  CL128 HEK-293T C27(1.09); C34(1.09)  LDD1603  [14]
 LDCM0400  CL13 HEK-293T C218(1.19)  LDD1604  [14]
 LDCM0401  CL14 HEK-293T C218(0.96)  LDD1605  [14]
 LDCM0402  CL15 HEK-293T C218(1.06)  LDD1606  [14]
 LDCM0403  CL16 HEK-293T C27(1.09); C34(1.09)  LDD1607  [14]
 LDCM0406  CL19 HEK-293T C218(1.19)  LDD1610  [14]
 LDCM0407  CL2 HEK-293T C218(1.13)  LDD1611  [14]
 LDCM0411  CL23 HEK-293T C218(1.27)  LDD1615  [14]
 LDCM0413  CL25 HEK-293T C218(0.94)  LDD1617  [14]
 LDCM0414  CL26 HEK-293T C218(1.09)  LDD1618  [14]
 LDCM0415  CL27 HEK-293T C218(1.08)  LDD1619  [14]
 LDCM0416  CL28 HEK-293T C27(1.00); C34(1.00)  LDD1620  [14]
 LDCM0418  CL3 HEK-293T C218(1.24)  LDD1622  [14]
 LDCM0420  CL31 HEK-293T C218(1.02)  LDD1624  [14]
 LDCM0424  CL35 HEK-293T C218(1.06)  LDD1628  [14]
 LDCM0426  CL37 HEK-293T C218(0.99)  LDD1630  [14]
 LDCM0428  CL39 HEK-293T C218(0.74)  LDD1632  [14]
 LDCM0429  CL4 HEK-293T C27(1.07); C34(1.07)  LDD1633  [14]
 LDCM0430  CL40 HEK-293T C27(0.96); C34(0.96)  LDD1634  [14]
 LDCM0433  CL43 HEK-293T C218(1.23)  LDD1637  [14]
 LDCM0437  CL47 HEK-293T C218(1.23)  LDD1641  [14]
 LDCM0439  CL49 HEK-293T C218(1.30)  LDD1643  [14]
 LDCM0441  CL50 HEK-293T C218(1.15)  LDD1645  [14]
 LDCM0443  CL52 HEK-293T C27(1.04); C34(1.04)  LDD1646  [14]
 LDCM0446  CL55 HEK-293T C218(1.04)  LDD1649  [14]
 LDCM0450  CL59 HEK-293T C218(1.12)  LDD1653  [14]
 LDCM0453  CL61 HEK-293T C218(1.14)  LDD1656  [14]
 LDCM0454  CL62 HEK-293T C218(1.05)  LDD1657  [14]
 LDCM0455  CL63 HEK-293T C218(1.11)  LDD1658  [14]
 LDCM0456  CL64 HEK-293T C27(0.97); C34(0.97)  LDD1659  [14]
 LDCM0459  CL67 HEK-293T C218(1.17)  LDD1662  [14]
 LDCM0462  CL7 HEK-293T C218(1.23)  LDD1665  [14]
 LDCM0464  CL71 HEK-293T C218(1.06)  LDD1667  [14]
 LDCM0466  CL73 HEK-293T C218(0.96)  LDD1669  [14]
 LDCM0467  CL74 HEK-293T C218(1.24)  LDD1670  [14]
 LDCM0469  CL76 HEK-293T C27(0.99); C34(0.99)  LDD1672  [14]
 LDCM0472  CL79 HEK-293T C218(1.08)  LDD1675  [14]
 LDCM0477  CL83 HEK-293T C218(0.92)  LDD1680  [14]
 LDCM0479  CL85 HEK-293T C218(1.02)  LDD1682  [14]
 LDCM0480  CL86 HEK-293T C218(1.11)  LDD1683  [14]
 LDCM0481  CL87 HEK-293T C218(1.15)  LDD1684  [14]
 LDCM0482  CL88 HEK-293T C27(1.12); C34(1.12)  LDD1685  [14]
 LDCM0486  CL91 HEK-293T C218(1.22)  LDD1689  [14]
 LDCM0490  CL95 HEK-293T C218(0.90)  LDD1693  [14]
 LDCM0492  CL97 HEK-293T C218(1.11)  LDD1695  [14]
 LDCM0493  CL98 HEK-293T C218(0.89)  LDD1696  [14]
 LDCM0494  CL99 HEK-293T C218(0.96)  LDD1697  [14]
 LDCM0495  E2913 HEK-293T C218(1.08)  LDD1698  [14]
 LDCM0468  Fragment33 HEK-293T C218(1.26)  LDD1671  [14]
 LDCM0427  Fragment51 HEK-293T C218(1.04)  LDD1631  [14]
 LDCM0116  HHS-0101 DM93 Y68(0.48)  LDD0264  [6]
 LDCM0117  HHS-0201 DM93 Y68(0.64)  LDD0265  [6]
 LDCM0118  HHS-0301 DM93 Y68(0.48)  LDD0266  [6]
 LDCM0119  HHS-0401 DM93 Y68(0.49)  LDD0267  [6]
 LDCM0120  HHS-0701 DM93 Y68(0.53)  LDD0268  [6]
 LDCM0107  IAA HeLa N.A.  LDD0221  [12]
 LDCM0123  JWB131 DM93 Y233(1.31)  LDD0285  [5]
 LDCM0124  JWB142 DM93 Y233(0.82)  LDD0286  [5]
 LDCM0125  JWB146 DM93 Y233(1.31)  LDD0287  [5]
 LDCM0126  JWB150 DM93 Y233(3.46); Y68(0.94)  LDD0288  [5]
 LDCM0127  JWB152 DM93 Y233(2.85); Y68(0.78)  LDD0289  [5]
 LDCM0128  JWB198 DM93 Y233(1.57)  LDD0290  [5]
 LDCM0129  JWB202 DM93 Y233(0.55)  LDD0291  [5]
 LDCM0130  JWB211 DM93 Y233(1.32)  LDD0292  [5]
 LDCM0022  KB02 22RV1 C43(1.32); C53(1.13); C20(1.00)  LDD2243  [3]
 LDCM0023  KB03 22RV1 C43(1.60); C53(1.35); C20(1.12)  LDD2660  [3]
 LDCM0024  KB05 COLO792 C53(0.80)  LDD3310  [3]
 LDCM0109  NEM HeLa N.A.  LDD0226  [12]
 LDCM0496  Nucleophilic fragment 11a MDA-MB-231 C182(1.53); C43(0.72)  LDD2089  [4]
 LDCM0506  Nucleophilic fragment 16a MDA-MB-231 C182(0.90)  LDD2099  [4]
 LDCM0514  Nucleophilic fragment 20a MDA-MB-231 C182(0.85)  LDD2107  [4]
 LDCM0211  Nucleophilic fragment 3b MDA-MB-231 C182(1.22)  LDD1700  [4]
 LDCM0546  Nucleophilic fragment 40 MDA-MB-231 C182(0.55)  LDD2140  [4]
 LDCM0552  Nucleophilic fragment 6a MDA-MB-231 C182(0.64)  LDD2146  [4]
 LDCM0556  Nucleophilic fragment 8a MDA-MB-231 C182(1.39)  LDD2150  [4]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Other
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
U1 small nuclear ribonucleoprotein A (SNRPA) RRM U1 A/B'' family P09012

References

1 Chemoproteomic profiling of kinases in live cells using electrophilic sulfonyl triazole probes. Chem Sci. 2021 Jan 21;12(9):3295-3307. doi: 10.1039/d0sc06623k.
2 Oxidant-Induced Bioconjugation for Protein Labeling in Live Cells. ACS Chem Biol. 2023 Jan 20;18(1):112-122. doi: 10.1021/acschembio.2c00740. Epub 2022 Dec 21.
3 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
4 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
5 Chemoproteomic profiling of kinases in live cells using electrophilic sulfonyl triazole probes. Chem Sci. 2021 Jan 21;12(9):3295-3307. doi: 10.1039/d0sc06623k.
6 Discovery of a Cell-Active SuTEx Ligand of Prostaglandin Reductase 2. Chembiochem. 2021 Jun 15;22(12):2134-2139. doi: 10.1002/cbic.202000879. Epub 2021 Apr 29.
7 Global targeting of functional tyrosines using sulfur-triazole exchange chemistry. Nat Chem Biol. 2020 Feb;16(2):150-159. doi: 10.1038/s41589-019-0404-5. Epub 2019 Nov 25.
8 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
9 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
10 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
11 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
12 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
13 Chemoproteomic capture of RNA binding activity in living cells. Nat Commun. 2023 Oct 7;14(1):6282. doi: 10.1038/s41467-023-41844-z.
Mass spectrometry data entry: PXD044625
14 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402