Details of the Target

General Information of Target

Target ID LDTP06495
Target Name Upstream stimulatory factor 2 (USF2)
Gene Name USF2
Gene ID 7392
Synonyms
BHLHB12; Upstream stimulatory factor 2; Class B basic helix-loop-helix protein 12; bHLHb12; FOS-interacting protein; FIP; Major late transcription factor 2; Upstream transcription factor 2
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MDMLDPGLDPAASATAAAAASHDKGPEAEEGVELQEGGDGPGAEEQTAVAITSVQQAAFG
DHNIQYQFRTETNGGQVTYRVVQVTDGQLDGQGDTAGAVSVVSTAAFAGGQQAVTQVGVD
GAAQRPGPAAASVPPGPAAPFPLAVIQNPFSNGGSPAAEAVSGEARFAYFPASSVGDTTA
VSVQTTDQSLQAGGQFYVMMTPQDVLQTGTQRTIAPRTHPYSPKIDGTRTPRDERRRAQH
NEVERRRRDKINNWIVQLSKIIPDCNADNSKTGASKGGILSKACDYIRELRQTNQRMQET
FKEAERLQMDNELLRQQIEELKNENALLRAQLQQHNLEMVGEGTRQ
Target Bioclass
Transcription factor
Subcellular location
Nucleus
Function Transcription factor that binds to a symmetrical DNA sequence (E-boxes) (5'-CACGTG-3') that is found in a variety of viral and cellular promoters.
Uniprot ID
Q15853
Ensemble ID
ENST00000222305.8
HGNC ID
HGNC:12594

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
G361 SNV: p.P231S .
HCT15 SNV: p.G118D .
JHH4 SNV: p.S100I .
JURKAT SNV: p.V183I .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 8 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
STPyne
 Probe Info  		K250(10.00)  LDD0277  [1]
Probe 1
 Probe Info  		Y79(72.13)  LDD3495  [2]
Alkyne-RA190
 Probe Info  		2.87  LDD0303  [3]
DBIA
 Probe Info  		C284(21.48)  LDD0209  [4]
IA-alkyne
 Probe Info  		N.A.  LDD0165  [5]
Lodoacetamide azide
 Probe Info  		N.A.  LDD0037  [6]
TFBX
 Probe Info  		N.A.  LDD0027  [7]
IPM
 Probe Info  		C284(0.00); C265(0.00)  LDD0147  [7]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0214  AC1 HCT 116 C265(1.15)  LDD0531  [8]
 LDCM0215  AC10 HEK-293T C265(0.94)  LDD1508  [9]
 LDCM0216  AC100 HCT 116 C265(1.01)  LDD0533  [8]
 LDCM0217  AC101 HCT 116 C265(0.96)  LDD0534  [8]
 LDCM0218  AC102 HCT 116 C265(0.99)  LDD0535  [8]
 LDCM0219  AC103 HCT 116 C265(0.95)  LDD0536  [8]
 LDCM0220  AC104 HCT 116 C265(0.99)  LDD0537  [8]
 LDCM0221  AC105 HCT 116 C265(0.94)  LDD0538  [8]
 LDCM0222  AC106 HCT 116 C265(0.94)  LDD0539  [8]
 LDCM0223  AC107 HCT 116 C265(0.97)  LDD0540  [8]
 LDCM0224  AC108 HCT 116 C265(0.96)  LDD0541  [8]
 LDCM0225  AC109 HCT 116 C265(0.95)  LDD0542  [8]
 LDCM0226  AC11 HEK-293T C265(1.05)  LDD1509  [9]
 LDCM0227  AC110 HCT 116 C265(0.98)  LDD0544  [8]
 LDCM0228  AC111 HCT 116 C265(0.98)  LDD0545  [8]
 LDCM0229  AC112 HCT 116 C265(1.05)  LDD0546  [8]
 LDCM0237  AC12 HEK-293T C265(0.84)  LDD1510  [9]
 LDCM0259  AC14 HEK-293T C265(0.96)  LDD1512  [9]
 LDCM0270  AC15 HEK-293T C265(1.12)  LDD1513  [9]
 LDCM0276  AC17 HEK-293T C265(0.92)  LDD1515  [9]
 LDCM0277  AC18 HEK-293T C265(1.02)  LDD1516  [9]
 LDCM0278  AC19 HEK-293T C265(1.12)  LDD1517  [9]
 LDCM0279  AC2 HCT 116 C265(1.16)  LDD0596  [8]
 LDCM0280  AC20 HEK-293T C265(1.30)  LDD1519  [9]
 LDCM0281  AC21 HEK-293T C265(0.94)  LDD1520  [9]
 LDCM0282  AC22 HEK-293T C265(1.00)  LDD1521  [9]
 LDCM0283  AC23 HEK-293T C265(1.10)  LDD1522  [9]
 LDCM0284  AC24 HEK-293T C265(0.99)  LDD1523  [9]
 LDCM0285  AC25 HEK-293T C265(1.00)  LDD1524  [9]
 LDCM0286  AC26 HEK-293T C265(1.07)  LDD1525  [9]
 LDCM0287  AC27 HEK-293T C265(0.97)  LDD1526  [9]
 LDCM0288  AC28 HEK-293T C265(0.96)  LDD1527  [9]
 LDCM0289  AC29 HEK-293T C265(1.08)  LDD1528  [9]
 LDCM0290  AC3 HCT 116 C265(1.15)  LDD0607  [8]
 LDCM0291  AC30 HEK-293T C265(1.03)  LDD1530  [9]
 LDCM0292  AC31 HEK-293T C265(1.11)  LDD1531  [9]
 LDCM0293  AC32 HEK-293T C265(1.03)  LDD1532  [9]
 LDCM0294  AC33 HEK-293T C265(0.86)  LDD1533  [9]
 LDCM0295  AC34 HEK-293T C265(1.03)  LDD1534  [9]
 LDCM0296  AC35 HEK-293T C265(0.96)  LDD1535  [9]
 LDCM0297  AC36 HEK-293T C265(0.81)  LDD1536  [9]
 LDCM0298  AC37 HEK-293T C265(0.89)  LDD1537  [9]
 LDCM0299  AC38 HEK-293T C265(1.04)  LDD1538  [9]
 LDCM0300  AC39 HEK-293T C265(1.00)  LDD1539  [9]
 LDCM0301  AC4 HCT 116 C265(1.30)  LDD0618  [8]
 LDCM0302  AC40 HEK-293T C265(0.93)  LDD1541  [9]
 LDCM0303  AC41 HEK-293T C265(1.03)  LDD1542  [9]
 LDCM0304  AC42 HEK-293T C265(0.96)  LDD1543  [9]
 LDCM0305  AC43 HEK-293T C265(1.28)  LDD1544  [9]
 LDCM0306  AC44 HEK-293T C265(1.03)  LDD1545  [9]
 LDCM0307  AC45 HEK-293T C265(0.97)  LDD1546  [9]
 LDCM0308  AC46 HEK-293T C265(0.94)  LDD1547  [9]
 LDCM0309  AC47 HEK-293T C265(1.34)  LDD1548  [9]
 LDCM0310  AC48 HEK-293T C265(1.02)  LDD1549  [9]
 LDCM0311  AC49 HEK-293T C265(0.92)  LDD1550  [9]
 LDCM0312  AC5 HCT 116 C265(1.23)  LDD0629  [8]
 LDCM0313  AC50 HEK-293T C265(0.98)  LDD1552  [9]
 LDCM0314  AC51 HEK-293T C265(1.00)  LDD1553  [9]
 LDCM0315  AC52 HEK-293T C265(0.90)  LDD1554  [9]
 LDCM0316  AC53 HEK-293T C265(0.92)  LDD1555  [9]
 LDCM0317  AC54 HEK-293T C265(0.87)  LDD1556  [9]
 LDCM0318  AC55 HEK-293T C265(1.01)  LDD1557  [9]
 LDCM0319  AC56 HEK-293T C265(1.05)  LDD1558  [9]
 LDCM0320  AC57 HEK-293T C265(0.89)  LDD1559  [9]
 LDCM0321  AC58 HEK-293T C265(0.97)  LDD1560  [9]
 LDCM0322  AC59 HEK-293T C265(0.81)  LDD1561  [9]
 LDCM0323  AC6 HEK-293T C265(0.95)  LDD1562  [9]
 LDCM0324  AC60 HEK-293T C265(0.90)  LDD1563  [9]
 LDCM0325  AC61 HEK-293T C265(0.93)  LDD1564  [9]
 LDCM0326  AC62 HEK-293T C265(1.02)  LDD1565  [9]
 LDCM0327  AC63 HEK-293T C265(1.43)  LDD1566  [9]
 LDCM0328  AC64 HEK-293T C265(0.96)  LDD1567  [9]
 LDCM0334  AC7 HEK-293T C265(1.07)  LDD1568  [9]
 LDCM0345  AC8 HEK-293T C265(1.04)  LDD1569  [9]
 LDCM0349  AC83 HCT 116 C265(1.19)  LDD0666  [8]
 LDCM0350  AC84 HCT 116 C265(1.06)  LDD0667  [8]
 LDCM0351  AC85 HCT 116 C265(1.07)  LDD0668  [8]
 LDCM0352  AC86 HCT 116 C265(1.16)  LDD0669  [8]
 LDCM0353  AC87 HCT 116 C265(1.06)  LDD0670  [8]
 LDCM0354  AC88 HCT 116 C265(1.22)  LDD0671  [8]
 LDCM0355  AC89 HCT 116 C265(1.19)  LDD0672  [8]
 LDCM0357  AC90 HCT 116 C265(1.19)  LDD0674  [8]
 LDCM0358  AC91 HCT 116 C265(1.02)  LDD0675  [8]
 LDCM0359  AC92 HCT 116 C265(0.94)  LDD0676  [8]
 LDCM0360  AC93 HCT 116 C265(1.05)  LDD0677  [8]
 LDCM0361  AC94 HCT 116 C265(1.09)  LDD0678  [8]
 LDCM0362  AC95 HCT 116 C265(1.14)  LDD0679  [8]
 LDCM0363  AC96 HCT 116 C265(1.04)  LDD0680  [8]
 LDCM0364  AC97 HCT 116 C265(1.03)  LDD0681  [8]
 LDCM0365  AC98 HCT 116 C265(1.06)  LDD0682  [8]
 LDCM0366  AC99 HCT 116 C265(0.95)  LDD0683  [8]
 LDCM0248  AKOS034007472 HEK-293T C265(0.92)  LDD1511  [9]
 LDCM0356  AKOS034007680 HEK-293T C265(0.86)  LDD1570  [9]
 LDCM0275  AKOS034007705 HEK-293T C265(0.98)  LDD1514  [9]
 LDCM0367  CL1 HEK-293T C265(0.95)  LDD1571  [9]
 LDCM0368  CL10 HEK-293T C265(1.05)  LDD1572  [9]
 LDCM0369  CL100 HCT 116 C265(1.11)  LDD0686  [8]
 LDCM0370  CL101 HEK-293T C265(1.16)  LDD1574  [9]
 LDCM0371  CL102 HEK-293T C265(1.03)  LDD1575  [9]
 LDCM0373  CL104 HEK-293T C265(0.76)  LDD1577  [9]
 LDCM0374  CL105 HEK-293T C265(1.27)  LDD1578  [9]
 LDCM0375  CL106 HEK-293T C265(1.01)  LDD1579  [9]
 LDCM0377  CL108 HEK-293T C265(0.76)  LDD1581  [9]
 LDCM0378  CL109 HEK-293T C265(1.31)  LDD1582  [9]
 LDCM0379  CL11 HEK-293T C265(1.02)  LDD1583  [9]
 LDCM0380  CL110 HEK-293T C265(1.00)  LDD1584  [9]
 LDCM0382  CL112 HEK-293T C265(0.82)  LDD1586  [9]
 LDCM0383  CL113 HEK-293T C265(0.85)  LDD1587  [9]
 LDCM0384  CL114 HEK-293T C265(1.02)  LDD1588  [9]
 LDCM0386  CL116 HEK-293T C265(0.92)  LDD1590  [9]
 LDCM0387  CL117 HEK-293T C265(0.93)  LDD1591  [9]
 LDCM0388  CL118 HEK-293T C265(1.00)  LDD1592  [9]
 LDCM0390  CL12 HEK-293T C265(1.05)  LDD1594  [9]
 LDCM0391  CL120 HEK-293T C265(0.78)  LDD1595  [9]
 LDCM0392  CL121 HEK-293T C265(0.98)  LDD1596  [9]
 LDCM0393  CL122 HEK-293T C265(0.96)  LDD1597  [9]
 LDCM0395  CL124 HEK-293T C265(0.86)  LDD1599  [9]
 LDCM0396  CL125 HEK-293T C265(0.91)  LDD1600  [9]
 LDCM0397  CL126 HEK-293T C265(0.92)  LDD1601  [9]
 LDCM0399  CL128 HEK-293T C265(0.82)  LDD1603  [9]
 LDCM0400  CL13 HEK-293T C265(1.11)  LDD1604  [9]
 LDCM0401  CL14 HEK-293T C265(0.85)  LDD1605  [9]
 LDCM0403  CL16 HEK-293T C265(0.89)  LDD1607  [9]
 LDCM0404  CL17 HCT 116 C265(1.04)  LDD0721  [8]
 LDCM0405  CL18 HCT 116 C265(0.90)  LDD0722  [8]
 LDCM0406  CL19 HCT 116 C265(0.91)  LDD0723  [8]
 LDCM0407  CL2 HEK-293T C265(0.93)  LDD1611  [9]
 LDCM0408  CL20 HCT 116 C265(0.90)  LDD0725  [8]
 LDCM0409  CL21 HCT 116 C265(0.82)  LDD0726  [8]
 LDCM0410  CL22 HCT 116 C265(0.83)  LDD0727  [8]
 LDCM0411  CL23 HCT 116 C265(0.76)  LDD0728  [8]
 LDCM0412  CL24 HCT 116 C265(0.87)  LDD0729  [8]
 LDCM0413  CL25 HCT 116 C265(0.79)  LDD0730  [8]
 LDCM0414  CL26 HCT 116 C265(0.90)  LDD0731  [8]
 LDCM0415  CL27 HCT 116 C265(0.88)  LDD0732  [8]
 LDCM0416  CL28 HCT 116 C265(0.74)  LDD0733  [8]
 LDCM0417  CL29 HCT 116 C265(0.88)  LDD0734  [8]
 LDCM0419  CL30 HCT 116 C265(0.81)  LDD0736  [8]
 LDCM0420  CL31 HCT 116 C265(1.10)  LDD0737  [8]
 LDCM0421  CL32 HCT 116 C265(0.86)  LDD0738  [8]
 LDCM0422  CL33 HCT 116 C265(1.30)  LDD0739  [8]
 LDCM0423  CL34 HCT 116 C265(1.13)  LDD0740  [8]
 LDCM0424  CL35 HCT 116 C265(1.02)  LDD0741  [8]
 LDCM0425  CL36 HCT 116 C265(1.04)  LDD0742  [8]
 LDCM0426  CL37 HCT 116 C265(0.81)  LDD0743  [8]
 LDCM0428  CL39 HCT 116 C265(1.09)  LDD0745  [8]
 LDCM0429  CL4 HEK-293T C265(1.00)  LDD1633  [9]
 LDCM0430  CL40 HCT 116 C265(0.92)  LDD0747  [8]
 LDCM0431  CL41 HCT 116 C265(1.00)  LDD0748  [8]
 LDCM0432  CL42 HCT 116 C265(0.83)  LDD0749  [8]
 LDCM0433  CL43 HCT 116 C265(1.14)  LDD0750  [8]
 LDCM0434  CL44 HCT 116 C265(0.77)  LDD0751  [8]
 LDCM0435  CL45 HCT 116 C265(0.79)  LDD0752  [8]
 LDCM0436  CL46 HEK-293T C265(0.85)  LDD1640  [9]
 LDCM0437  CL47 HEK-293T C265(1.34)  LDD1641  [9]
 LDCM0438  CL48 HEK-293T C265(0.95)  LDD1642  [9]
 LDCM0439  CL49 HEK-293T C265(1.08)  LDD1643  [9]
 LDCM0440  CL5 HEK-293T C265(0.82)  LDD1644  [9]
 LDCM0441  CL50 HEK-293T C265(0.93)  LDD1645  [9]
 LDCM0443  CL52 HEK-293T C265(0.74)  LDD1646  [9]
 LDCM0444  CL53 HEK-293T C265(1.00)  LDD1647  [9]
 LDCM0445  CL54 HEK-293T C265(1.01)  LDD1648  [9]
 LDCM0446  CL55 HEK-293T C265(0.86)  LDD1649  [9]
 LDCM0447  CL56 HEK-293T C265(0.93)  LDD1650  [9]
 LDCM0448  CL57 HEK-293T C265(0.93)  LDD1651  [9]
 LDCM0449  CL58 HEK-293T C265(0.90)  LDD1652  [9]
 LDCM0450  CL59 HEK-293T C265(1.05)  LDD1653  [9]
 LDCM0451  CL6 HEK-293T C265(1.04)  LDD1654  [9]
 LDCM0452  CL60 HEK-293T C265(1.01)  LDD1655  [9]
 LDCM0453  CL61 HEK-293T C265(1.08)  LDD1656  [9]
 LDCM0454  CL62 HEK-293T C265(0.90)  LDD1657  [9]
 LDCM0456  CL64 HEK-293T C265(0.88)  LDD1659  [9]
 LDCM0457  CL65 HEK-293T C265(0.92)  LDD1660  [9]
 LDCM0458  CL66 HEK-293T C265(0.98)  LDD1661  [9]
 LDCM0459  CL67 HEK-293T C265(1.01)  LDD1662  [9]
 LDCM0460  CL68 HEK-293T C265(0.93)  LDD1663  [9]
 LDCM0461  CL69 HEK-293T C265(0.90)  LDD1664  [9]
 LDCM0462  CL7 HEK-293T C265(1.04)  LDD1665  [9]
 LDCM0463  CL70 HEK-293T C265(0.86)  LDD1666  [9]
 LDCM0464  CL71 HEK-293T C265(0.92)  LDD1667  [9]
 LDCM0465  CL72 HEK-293T C265(0.97)  LDD1668  [9]
 LDCM0466  CL73 HEK-293T C265(0.92)  LDD1669  [9]
 LDCM0467  CL74 HEK-293T C265(0.96)  LDD1670  [9]
 LDCM0469  CL76 HEK-293T C265(0.79)  LDD1672  [9]
 LDCM0470  CL77 HEK-293T C265(1.00)  LDD1673  [9]
 LDCM0471  CL78 HEK-293T C265(0.96)  LDD1674  [9]
 LDCM0472  CL79 HEK-293T C265(0.92)  LDD1675  [9]
 LDCM0473  CL8 HEK-293T C265(1.56)  LDD1676  [9]
 LDCM0474  CL80 HEK-293T C265(0.90)  LDD1677  [9]
 LDCM0475  CL81 HEK-293T C265(0.98)  LDD1678  [9]
 LDCM0476  CL82 HEK-293T C265(0.87)  LDD1679  [9]
 LDCM0477  CL83 HEK-293T C265(1.06)  LDD1680  [9]
 LDCM0478  CL84 HEK-293T C265(0.99)  LDD1681  [9]
 LDCM0479  CL85 HEK-293T C265(0.77)  LDD1682  [9]
 LDCM0480  CL86 HEK-293T C265(0.88)  LDD1683  [9]
 LDCM0482  CL88 HEK-293T C265(1.16)  LDD1685  [9]
 LDCM0483  CL89 HEK-293T C265(1.06)  LDD1686  [9]
 LDCM0484  CL9 HEK-293T C265(0.98)  LDD1687  [9]
 LDCM0485  CL90 HEK-293T C265(1.06)  LDD1688  [9]
 LDCM0486  CL91 HCT 116 C265(1.17)  LDD0803  [8]
 LDCM0487  CL92 HCT 116 C265(0.94)  LDD0804  [8]
 LDCM0488  CL93 HCT 116 C265(1.18)  LDD0805  [8]
 LDCM0489  CL94 HCT 116 C265(1.07)  LDD0806  [8]
 LDCM0490  CL95 HCT 116 C265(1.05)  LDD0807  [8]
 LDCM0491  CL96 HCT 116 C265(1.12)  LDD0808  [8]
 LDCM0492  CL97 HCT 116 C265(1.16)  LDD0809  [8]
 LDCM0493  CL98 HCT 116 C265(1.23)  LDD0810  [8]
 LDCM0494  CL99 HCT 116 C265(1.11)  LDD0811  [8]
 LDCM0427  Fragment51 HCT 116 C265(0.49)  LDD0744  [8]
 LDCM0022  KB02 HEK-293T C265(0.90)  LDD1492  [9]
 LDCM0023  KB03 Jurkat C284(21.48)  LDD0209  [4]
 LDCM0024  KB05 HEK-293T C265(0.90)  LDD1502  [9]
 LDCM0131  RA190 SK-MEL-5 2.87  LDD0303  [3]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Transcription factor
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Transcriptional enhancer factor TEF-4 (TEAD2) . Q15562
Other
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Leucine zipper putative tumor suppressor 2 (LZTS2) LZTS2 family Q9BRK4

References

1 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
2 An Azo Coupling-Based Chemoproteomic Approach to Systematically Profile the Tyrosine Reactivity in the Human Proteome. Anal Chem. 2021 Jul 27;93(29):10334-10342. doi: 10.1021/acs.analchem.1c01935. Epub 2021 Jul 12.
3 Physical and Functional Analysis of the Putative Rpn13 Inhibitor RA190. Cell Chem Biol. 2020 Nov 19;27(11):1371-1382.e6. doi: 10.1016/j.chembiol.2020.08.007. Epub 2020 Aug 27.
4 Covalent Inhibition by a Natural Product-Inspired Latent Electrophile. J Am Chem Soc. 2023 May 24;145(20):11097-11109. doi: 10.1021/jacs.3c00598. Epub 2023 May 15.
5 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
6 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
7 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
8 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
9 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402