Details of the Target

General Information of Target

Target ID LDTP05846
Target Name Phosducin-like protein (PDCL)
Gene Name PDCL
Gene ID 5082
Synonyms
PHLOP1; PhLP1; Phosducin-like protein; PHLP
3D Structure
Download
2D Sequence (FASTA)
Download
3D Structure (PDB)
Download
Sequence
MTTLDDKLLGEKLQYYYSSSEDEDSDHEDKDRGRCAPASSSVPAEAELAGEGISVNTGPK
GVINDWRRFKQLETEQREEQCREMERLIKKLSMTCRSHLDEEEEQQKQKDLQEKISGKMT
LKEFAIMNEDQDDEEFLQQYRKQRMEEMRQQLHKGPQFKQVFEISSGEGFLDMIDKEQKS
IVIMVHIYEDGIPGTEAMNGCMICLAAEYPAVKFCKVKSSVIGASSQFTRNALPALLIYK
GGELIGNFVRVTDQLGDDFFAVDLEAFLQEFGLLPEKEVLVLTSVRNSATCHSEDSDLEI
D
Target Bioclass
Other
Family
Phosducin family
Subcellular location
Cell projection, cilium
Function
Acts as a positive regulator of hedgehog signaling and regulates ciliary function.; [Isoform 1]: Functions as a co-chaperone for CCT in the assembly of heterotrimeric G protein complexes, facilitates the assembly of both Gbeta-Ggamma and RGS-Gbeta5 heterodimers.; [Isoform 2]: Acts as a negative regulator of heterotrimeric G proteins assembly by trapping the preloaded G beta subunits inside the CCT chaperonin.
Uniprot ID
Q13371
Ensemble ID
ENST00000259467.9
HGNC ID
HGNC:8770

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
CAL33 SNV: p.V62M DBIA    Probe Info 
COLO320 SNV: p.A36P DBIA    Probe Info 
RKO SNV: p.V161F .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 13 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
BTD
 Probe Info  		C95(0.90)  LDD2099  [1]
Curcusone 37
 Probe Info  		2.45  LDD0188  [2]
DBIA
 Probe Info  		C291(7.99)  LDD0204  [3]
Acrolein
 Probe Info  		N.A.  LDD0221  [4]
IA-alkyne
 Probe Info  		N.A.  LDD0036  [5]
Lodoacetamide azide
 Probe Info  		N.A.  LDD0037  [5]
NAIA_4
 Probe Info  		N.A.  LDD2226  [6]
NAIA_5
 Probe Info  		N.A.  LDD2225  [6]
TFBX
 Probe Info  		N.A.  LDD0027  [7]
IPM
 Probe Info  		N.A.  LDD0005  [8]
VSF
 Probe Info  		N.A.  LDD0007  [8]
Phosphinate-6
 Probe Info  		N.A.  LDD0018  [9]
AOyne
 Probe Info  		15.00  LDD0443  [10]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0026  4SU-RNA+native RNA HEK-293T C95(9.07)  LDD0372  [11]
 LDCM0215  AC10 HEK-293T C35(1.09)  LDD1508  [12]
 LDCM0226  AC11 HEK-293T C35(1.12)  LDD1509  [12]
 LDCM0237  AC12 HEK-293T C35(0.95); C81(1.13); C95(1.12)  LDD1510  [12]
 LDCM0259  AC14 HEK-293T C35(0.97); C95(1.30)  LDD1512  [12]
 LDCM0270  AC15 HEK-293T C35(0.96); C95(1.13)  LDD1513  [12]
 LDCM0277  AC18 HEK-293T C35(0.97)  LDD1516  [12]
 LDCM0278  AC19 HEK-293T C35(1.50)  LDD1517  [12]
 LDCM0279  AC2 HEK-293T C35(0.91)  LDD1518  [12]
 LDCM0280  AC20 HEK-293T C35(0.97); C81(0.94); C95(1.18)  LDD1519  [12]
 LDCM0282  AC22 HEK-293T C35(1.08); C95(1.24)  LDD1521  [12]
 LDCM0283  AC23 HEK-293T C35(1.15); C95(1.32)  LDD1522  [12]
 LDCM0284  AC24 HEK-293T C35(0.97)  LDD1523  [12]
 LDCM0286  AC26 HEK-293T C35(0.98)  LDD1525  [12]
 LDCM0287  AC27 HEK-293T C35(0.91)  LDD1526  [12]
 LDCM0288  AC28 HEK-293T C35(0.95); C81(1.09); C95(1.14)  LDD1527  [12]
 LDCM0290  AC3 HEK-293T C35(1.03)  LDD1529  [12]
 LDCM0291  AC30 HEK-293T C35(0.98); C95(1.18)  LDD1530  [12]
 LDCM0292  AC31 HEK-293T C35(1.05); C95(1.32)  LDD1531  [12]
 LDCM0293  AC32 HEK-293T C35(0.89)  LDD1532  [12]
 LDCM0295  AC34 HEK-293T C35(1.06)  LDD1534  [12]
 LDCM0296  AC35 HEK-293T C35(0.87)  LDD1535  [12]
 LDCM0297  AC36 HEK-293T C35(0.94); C81(1.23); C95(1.23)  LDD1536  [12]
 LDCM0299  AC38 HEK-293T C35(1.14); C95(1.28)  LDD1538  [12]
 LDCM0300  AC39 HEK-293T C35(1.23); C95(1.25)  LDD1539  [12]
 LDCM0301  AC4 HEK-293T C35(0.97); C81(1.18); C95(1.18)  LDD1540  [12]
 LDCM0302  AC40 HEK-293T C35(0.91)  LDD1541  [12]
 LDCM0304  AC42 HEK-293T C35(1.01)  LDD1543  [12]
 LDCM0305  AC43 HEK-293T C35(0.93)  LDD1544  [12]
 LDCM0306  AC44 HEK-293T C35(1.02); C81(1.00); C95(1.11)  LDD1545  [12]
 LDCM0308  AC46 HEK-293T C35(1.08); C95(1.16)  LDD1547  [12]
 LDCM0309  AC47 HEK-293T C35(1.05); C95(1.23)  LDD1548  [12]
 LDCM0310  AC48 HEK-293T C35(1.05)  LDD1549  [12]
 LDCM0313  AC50 HEK-293T C35(0.98)  LDD1552  [12]
 LDCM0314  AC51 HEK-293T C35(0.95)  LDD1553  [12]
 LDCM0315  AC52 HEK-293T C35(1.00); C81(1.13); C95(1.12)  LDD1554  [12]
 LDCM0317  AC54 HEK-293T C35(0.94); C95(1.16)  LDD1556  [12]
 LDCM0318  AC55 HEK-293T C35(1.03); C95(1.20)  LDD1557  [12]
 LDCM0319  AC56 HEK-293T C35(0.98)  LDD1558  [12]
 LDCM0321  AC58 HEK-293T C35(0.95)  LDD1560  [12]
 LDCM0322  AC59 HEK-293T C35(1.01)  LDD1561  [12]
 LDCM0323  AC6 HEK-293T C35(1.03); C95(1.22)  LDD1562  [12]
 LDCM0324  AC60 HEK-293T C35(0.95); C81(1.04); C95(1.23)  LDD1563  [12]
 LDCM0326  AC62 HEK-293T C35(1.01); C95(1.23)  LDD1565  [12]
 LDCM0327  AC63 HEK-293T C35(1.11); C95(1.24)  LDD1566  [12]
 LDCM0328  AC64 HEK-293T C35(0.98)  LDD1567  [12]
 LDCM0334  AC7 HEK-293T C35(1.15); C95(1.50)  LDD1568  [12]
 LDCM0345  AC8 HEK-293T C35(0.97)  LDD1569  [12]
 LDCM0275  AKOS034007705 HEK-293T C35(0.98)  LDD1514  [12]
 LDCM0102  BDHI 8 Jurkat C291(7.99)  LDD0204  [3]
 LDCM0108  Chloroacetamide HeLa N.A.  LDD0222  [4]
 LDCM0632  CL-Sc Hep-G2 C35(1.83); C81(1.13)  LDD2227  [6]
 LDCM0368  CL10 HEK-293T C35(0.96); C95(0.56)  LDD1572  [12]
 LDCM0369  CL100 HEK-293T C35(1.00)  LDD1573  [12]
 LDCM0372  CL103 HEK-293T C35(1.05)  LDD1576  [12]
 LDCM0373  CL104 HEK-293T C35(0.90)  LDD1577  [12]
 LDCM0376  CL107 HEK-293T C35(1.01)  LDD1580  [12]
 LDCM0377  CL108 HEK-293T C35(1.00)  LDD1581  [12]
 LDCM0379  CL11 HEK-293T C35(1.07); C95(0.96)  LDD1583  [12]
 LDCM0381  CL111 HEK-293T C35(0.97)  LDD1585  [12]
 LDCM0382  CL112 HEK-293T C35(1.06)  LDD1586  [12]
 LDCM0385  CL115 HEK-293T C35(1.04)  LDD1589  [12]
 LDCM0386  CL116 HEK-293T C35(0.97)  LDD1590  [12]
 LDCM0389  CL119 HEK-293T C35(1.10)  LDD1593  [12]
 LDCM0390  CL12 HEK-293T C35(0.91)  LDD1594  [12]
 LDCM0391  CL120 HEK-293T C35(0.92)  LDD1595  [12]
 LDCM0394  CL123 HEK-293T C35(1.02)  LDD1598  [12]
 LDCM0395  CL124 HEK-293T C35(0.96)  LDD1599  [12]
 LDCM0398  CL127 HEK-293T C35(1.07)  LDD1602  [12]
 LDCM0399  CL128 HEK-293T C35(0.85)  LDD1603  [12]
 LDCM0402  CL15 HEK-293T C35(1.11)  LDD1606  [12]
 LDCM0403  CL16 HEK-293T C35(0.96)  LDD1607  [12]
 LDCM0405  CL18 HEK-293T C35(0.99)  LDD1609  [12]
 LDCM0406  CL19 HEK-293T C35(1.06)  LDD1610  [12]
 LDCM0408  CL20 HEK-293T C35(1.00); C81(0.34); C95(0.77)  LDD1612  [12]
 LDCM0410  CL22 HEK-293T C35(0.98); C95(0.57)  LDD1614  [12]
 LDCM0411  CL23 HEK-293T C35(1.21); C95(0.74)  LDD1615  [12]
 LDCM0412  CL24 HEK-293T C35(0.94)  LDD1616  [12]
 LDCM0415  CL27 HEK-293T C35(1.04)  LDD1619  [12]
 LDCM0416  CL28 HEK-293T C35(1.01)  LDD1620  [12]
 LDCM0418  CL3 HEK-293T C35(1.08)  LDD1622  [12]
 LDCM0419  CL30 HEK-293T C35(1.00)  LDD1623  [12]
 LDCM0420  CL31 HEK-293T C35(0.87)  LDD1624  [12]
 LDCM0421  CL32 HEK-293T C35(1.08); C81(0.39); C95(0.81)  LDD1625  [12]
 LDCM0423  CL34 HEK-293T C35(0.94); C95(0.57)  LDD1627  [12]
 LDCM0424  CL35 HEK-293T C35(1.07); C95(0.71)  LDD1628  [12]
 LDCM0425  CL36 HEK-293T C35(0.99)  LDD1629  [12]
 LDCM0428  CL39 HEK-293T C35(0.84)  LDD1632  [12]
 LDCM0429  CL4 HEK-293T C35(1.00)  LDD1633  [12]
 LDCM0430  CL40 HEK-293T C35(1.13)  LDD1634  [12]
 LDCM0432  CL42 HEK-293T C35(1.00)  LDD1636  [12]
 LDCM0433  CL43 HEK-293T C35(0.97)  LDD1637  [12]
 LDCM0434  CL44 HEK-293T C35(0.88); C81(0.47); C95(0.84)  LDD1638  [12]
 LDCM0436  CL46 HEK-293T C35(1.03); C95(0.60)  LDD1640  [12]
 LDCM0437  CL47 HEK-293T C35(1.13); C95(0.59)  LDD1641  [12]
 LDCM0438  CL48 HEK-293T C35(0.92)  LDD1642  [12]
 LDCM0443  CL52 HEK-293T C35(0.97)  LDD1646  [12]
 LDCM0445  CL54 HEK-293T C35(0.88)  LDD1648  [12]
 LDCM0446  CL55 HEK-293T C35(0.83)  LDD1649  [12]
 LDCM0447  CL56 HEK-293T C35(0.95); C81(0.38); C95(0.80)  LDD1650  [12]
 LDCM0449  CL58 HEK-293T C35(1.08); C95(0.60)  LDD1652  [12]
 LDCM0450  CL59 HEK-293T C35(1.08); C95(0.77)  LDD1653  [12]
 LDCM0451  CL6 HEK-293T C35(0.97)  LDD1654  [12]
 LDCM0452  CL60 HEK-293T C35(0.90)  LDD1655  [12]
 LDCM0455  CL63 HEK-293T C35(0.98)  LDD1658  [12]
 LDCM0456  CL64 HEK-293T C35(0.84)  LDD1659  [12]
 LDCM0458  CL66 HEK-293T C35(0.96)  LDD1661  [12]
 LDCM0459  CL67 HEK-293T C35(0.84)  LDD1662  [12]
 LDCM0460  CL68 HEK-293T C35(0.94); C81(0.40); C95(0.76)  LDD1663  [12]
 LDCM0462  CL7 HEK-293T C35(0.87)  LDD1665  [12]
 LDCM0463  CL70 HEK-293T C35(0.98); C95(0.65)  LDD1666  [12]
 LDCM0464  CL71 HEK-293T C35(1.25); C95(0.72)  LDD1667  [12]
 LDCM0465  CL72 HEK-293T C35(0.94)  LDD1668  [12]
 LDCM0469  CL76 HEK-293T C35(0.97)  LDD1672  [12]
 LDCM0471  CL78 HEK-293T C35(1.00)  LDD1674  [12]
 LDCM0472  CL79 HEK-293T C35(0.88)  LDD1675  [12]
 LDCM0473  CL8 HEK-293T C35(0.74); C81(0.56); C95(0.77)  LDD1676  [12]
 LDCM0474  CL80 HEK-293T C35(1.04); C81(0.54); C95(0.81)  LDD1677  [12]
 LDCM0476  CL82 HEK-293T C35(1.03); C95(0.69)  LDD1679  [12]
 LDCM0477  CL83 HEK-293T C35(0.94); C95(0.78)  LDD1680  [12]
 LDCM0478  CL84 HEK-293T C35(0.96)  LDD1681  [12]
 LDCM0481  CL87 HEK-293T C35(0.96)  LDD1684  [12]
 LDCM0482  CL88 HEK-293T C35(0.93)  LDD1685  [12]
 LDCM0485  CL90 HEK-293T C35(0.85)  LDD1688  [12]
 LDCM0486  CL91 HEK-293T C35(0.81)  LDD1689  [12]
 LDCM0487  CL92 HEK-293T C35(0.95); C81(0.82); C95(0.89)  LDD1690  [12]
 LDCM0489  CL94 HEK-293T C35(1.05); C95(0.78)  LDD1692  [12]
 LDCM0490  CL95 HEK-293T C35(1.18); C95(0.82)  LDD1693  [12]
 LDCM0491  CL96 HEK-293T C35(0.88)  LDD1694  [12]
 LDCM0494  CL99 HEK-293T C35(0.96)  LDD1697  [12]
 LDCM0033  Curcusone1d MCF-7 2.45  LDD0188  [2]
 LDCM0634  CY-0357 Hep-G2 C35(0.50)  LDD2228  [6]
 LDCM0495  E2913 HEK-293T C35(0.98)  LDD1698  [12]
 LDCM0213  Electrophilic fragment 2 MDA-MB-231 C95(1.53)  LDD1702  [1]
 LDCM0625  F8 Ramos C81(1.61); C35(0.98)  LDD2187  [13]
 LDCM0572  Fragment10 Ramos C81(1.18); C35(0.89)  LDD2189  [13]
 LDCM0573  Fragment11 Ramos C81(5.44); C35(0.86)  LDD2190  [13]
 LDCM0574  Fragment12 Ramos C81(0.57); C35(0.84)  LDD2191  [13]
 LDCM0575  Fragment13 Ramos C81(0.64); C35(0.69)  LDD2192  [13]
 LDCM0576  Fragment14 Ramos C81(0.95); C35(0.81)  LDD2193  [13]
 LDCM0579  Fragment20 Ramos C81(0.58); C35(0.82)  LDD2194  [13]
 LDCM0580  Fragment21 Ramos C81(0.74); C35(0.83)  LDD2195  [13]
 LDCM0582  Fragment23 Ramos C81(1.28); C35(0.86)  LDD2196  [13]
 LDCM0578  Fragment27 Ramos C81(1.01); C35(0.77)  LDD2197  [13]
 LDCM0586  Fragment28 Ramos C81(0.85); C35(1.17)  LDD2198  [13]
 LDCM0588  Fragment30 Ramos C81(0.74); C35(0.80)  LDD2199  [13]
 LDCM0589  Fragment31 Ramos C81(0.67); C35(0.69)  LDD2200  [13]
 LDCM0590  Fragment32 Ramos C81(1.20); C35(0.87)  LDD2201  [13]
 LDCM0468  Fragment33 HEK-293T C35(1.06)  LDD1671  [12]
 LDCM0596  Fragment38 Ramos C81(1.49); C35(0.75)  LDD2203  [13]
 LDCM0566  Fragment4 Ramos C81(1.27)  LDD2184  [13]
 LDCM0610  Fragment52 Ramos C81(0.75); C35(0.76)  LDD2204  [13]
 LDCM0614  Fragment56 Ramos C81(0.62); C35(0.91)  LDD2205  [13]
 LDCM0569  Fragment7 Ramos C81(1.41); C35(2.26)  LDD2186  [13]
 LDCM0571  Fragment9 Ramos C81(0.64); C35(0.82)  LDD2188  [13]
 LDCM0107  IAA HeLa N.A.  LDD0221  [4]
 LDCM0022  KB02 HEK-293T C35(1.12)  LDD1492  [12]
 LDCM0023  KB03 Jurkat C81(204.88)  LDD0209  [3]
 LDCM0024  KB05 SKMEL2 C35(1.18)  LDD3322  [14]
 LDCM0109  NEM HeLa N.A.  LDD0223  [4]
 LDCM0506  Nucleophilic fragment 16a MDA-MB-231 C95(0.90)  LDD2099  [1]
 LDCM0514  Nucleophilic fragment 20a MDA-MB-231 C95(1.36)  LDD2107  [1]
 LDCM0516  Nucleophilic fragment 21a MDA-MB-231 C95(0.94)  LDD2109  [1]
 LDCM0532  Nucleophilic fragment 29a MDA-MB-231 C95(0.84)  LDD2125  [1]
 LDCM0534  Nucleophilic fragment 30a MDA-MB-231 C95(0.93)  LDD2127  [1]
 LDCM0544  Nucleophilic fragment 39 MDA-MB-231 C95(1.25)  LDD2137  [1]
 LDCM0546  Nucleophilic fragment 40 MDA-MB-231 C95(1.05)  LDD2140  [1]
 LDCM0552  Nucleophilic fragment 6a MDA-MB-231 C95(0.88)  LDD2146  [1]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
26S proteasome regulatory subunit 8 (PSMC5) AAA ATPase family P62195
Kallikrein-6 (KLK6) Peptidase S1 family Q92876
Other
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
T-complex protein 1 subunit gamma (CCT3) TCP-1 chaperonin family P49368
Large ribosomal subunit protein uL11m (MRPL11) Universal ribosomal protein uL11 family Q9Y3B7

References

1 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
2 Total Synthesis and Target Identification of the Curcusone Diterpenes. J Am Chem Soc. 2021 Mar 24;143(11):4379-4386. doi: 10.1021/jacs.1c00557. Epub 2021 Mar 11.
3 Covalent Inhibition by a Natural Product-Inspired Latent Electrophile. J Am Chem Soc. 2023 May 24;145(20):11097-11109. doi: 10.1021/jacs.3c00598. Epub 2023 May 15.
4 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
5 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
6 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
7 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
8 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
9 DFT-Guided Discovery of Ethynyl-Triazolyl-Phosphinates as Modular Electrophiles for Chemoselective Cysteine Bioconjugation and Profiling. Angew Chem Int Ed Engl. 2022 Oct 10;61(41):e202205348. doi: 10.1002/anie.202205348. Epub 2022 Aug 22.
Mass spectrometry data entry: PXD033004
10 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
11 Chemoproteomic capture of RNA binding activity in living cells. Nat Commun. 2023 Oct 7;14(1):6282. doi: 10.1038/s41467-023-41844-z.
Mass spectrometry data entry: PXD044625
12 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
13 Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry. Methods Enzymol. 2023;679:295-322. doi: 10.1016/bs.mie.2022.07.037. Epub 2022 Sep 7.
Mass spectrometry data entry: PXD027578
14 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840