Details of the Target

General Information of Target

Target ID LDTP04109
Target Name Small ribosomal subunit protein uS4 (RPS9)
Gene Name RPS9
Gene ID 6203
Synonyms
Small ribosomal subunit protein uS4; 40S ribosomal protein S9
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MPVARSWVCRKTYVTPRRPFEKSRLDQELKLIGEYGLRNKREVWRVKFTLAKIRKAAREL
LTLDEKDPRRLFEGNALLRRLVRIGVLDEGKMKLDYILGLKIEDFLERRLQTQVFKLGLA
KSIHHARVLIRQRHIRVRKQVVNIPSFIVRLDSQKHIDFSLRSPYGGGRPGRVKRKNAKK
GQGGAGAGDDEEED
Target Bioclass
Other
Family
Universal ribosomal protein uS4 family
Subcellular location
Cytoplasm
Function
Component of the small ribosomal subunit. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome.
Uniprot ID
P46781
Ensemble ID
ENST00000302907.9
HGNC ID
HGNC:10442

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 32 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
m-APA
 Probe Info  		13.01  LDD0402  [1]
A-EBA
 Probe Info  		3.43  LDD0215  [2]
CHEMBL5175495
 Probe Info  		6.56  LDD0196  [3]
C-Sul
 Probe Info  		3.43  LDD0066  [4]
TH211
 Probe Info  		Y13(20.00); Y165(20.00); Y96(11.89)  LDD0257  [5]
TH214
 Probe Info  		Y13(12.50)  LDD0258  [5]
TH216
 Probe Info  		Y96(17.90); Y35(11.53); Y13(11.11)  LDD0259  [5]
YN-4
 Probe Info  		100.00  LDD0445  [6]
ONAyne
 Probe Info  		K11(0.00); K22(0.00); K30(0.00); K155(0.00)  LDD0273  [7]
OPA-S-S-alkyne
 Probe Info  		K30(1.01); K155(2.28); K52(2.39); K66(2.63)  LDD3494  [8]
Probe 1
 Probe Info  		Y35(11.99); Y165(584,252.31)  LDD3495  [9]
DBIA
 Probe Info  		C169(1.61)  LDD3432  [10]
AZ-9
 Probe Info  		10.00  LDD2154  [11]
HHS-475
 Probe Info  		Y96(0.65)  LDD0264  [12]
HHS-465
 Probe Info  		Y13(6.27); Y96(10.00)  LDD2237  [13]
Acrolein
 Probe Info  		N.A.  LDD0223  [14]
W1
 Probe Info  		K52(0.53); K139(0.59)  LDD0238  [15]
5E-2FA
 Probe Info  		N.A.  LDD2235  [16]
ATP probe
 Probe Info  		K139(0.00); K66(0.00); K30(0.00); K91(0.00)  LDD0199  [17]
ATP probe
 Probe Info  		K93(0.00); K30(0.00)  LDD0035  [18]
NAIA_5
 Probe Info  		N.A.  LDD2224  [19]
1d-yne
 Probe Info  		K91(0.00); K93(0.00)  LDD0356  [20]
NHS
 Probe Info  		K116(0.00); K139(0.00); K66(0.00); K155(0.00)  LDD0010  [21]
SF
 Probe Info  		K139(0.00); Y35(0.00); K155(0.00); K30(0.00)  LDD0028  [22]
STPyne
 Probe Info  		K155(0.00); K139(0.00)  LDD0009  [21]
Phosphinate-6
 Probe Info  		N.A.  LDD0018  [23]
Ox-W18
 Probe Info  		N.A.  LDD2175  [24]
1c-yne
 Probe Info  		K155(0.00); K93(0.00); K30(0.00)  LDD0228  [20]
AOyne
 Probe Info  		12.70  LDD0443  [25]
TER-AC
 Probe Info  		N.A.  LDD0426  [26]
THL-R
 Probe Info  		N.A.  LDD0077  [27]
HHS-482
 Probe Info  		Y13(1.05); Y96(1.11)  LDD2239  [13]
PAL-AfBPP Probe
Click To Hide/Show 1 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
STS-2
 Probe Info  		N.A.  LDD0138  [28]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0156  Aniline NCI-H1299 11.72  LDD0403  [1]
 LDCM0151  AZ-11 HeLa 10.00  LDD2154  [11]
 LDCM0116  HHS-0101 DM93 Y96(0.65)  LDD0264  [12]
 LDCM0117  HHS-0201 DM93 Y96(0.75)  LDD0265  [12]
 LDCM0118  HHS-0301 DM93 Y96(1.09)  LDD0266  [12]
 LDCM0119  HHS-0401 DM93 Y96(1.18)  LDD0267  [12]
 LDCM0120  HHS-0701 DM93 Y96(0.88)  LDD0268  [12]
 LDCM0022  KB02 HEC-1 C169(4.76)  LDD2350  [10]
 LDCM0023  KB03 HEC-1 C169(6.97)  LDD2767  [10]
 LDCM0024  KB05 SKN C169(1.61)  LDD3432  [10]
 LDCM0109  NEM HeLa N.A.  LDD0223  [14]
 LDCM0018  Orlistat Hep-G2 N.A.  LDD0077  [27]
 LDCM0111  W14 Hep-G2 K52(0.53); K139(0.59)  LDD0238  [15]
 LDCM0112  W16 Hep-G2 R54(0.95); Q140(1.07)  LDD0239  [15]
 LDCM0113  W17 Hep-G2 K139(0.50); Q140(0.50); R54(0.53)  LDD0240  [15]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Transcription factor
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Signal transducer and activator of transcription 3 (STAT3) Transcription factor STAT family P40763

The Drug(s) Related To This Target

Approved
Click To Hide/Show 1 Drug(s) Interacting with This Target
Drug Name Drug Type External ID
Artenimol . DB11638

References

1 Quantitative and Site-Specific Chemoproteomic Profiling of Targets of Acrolein. Chem Res Toxicol. 2019 Mar 18;32(3):467-473. doi: 10.1021/acs.chemrestox.8b00343. Epub 2019 Jan 15.
2 2-Ethynylbenzaldehyde-Based, Lysine-Targeting Irreversible Covalent Inhibitors for Protein Kinases and Nonkinases. J Am Chem Soc. 2023 Feb 12. doi: 10.1021/jacs.2c11595. Online ahead of print.
Mass spectrometry data entry: PXD037665
3 Charting the Chemical Space of Acrylamide-Based Inhibitors of zDHHC20. ACS Med Chem Lett. 2022 Sep 26;13(10):1648-1654. doi: 10.1021/acsmedchemlett.2c00336. eCollection 2022 Oct 13.
4 Low-Toxicity Sulfonium-Based Probes for Cysteine-Specific Profiling in Live Cells. Anal Chem. 2022 Mar 15;94(10):4366-4372. doi: 10.1021/acs.analchem.1c05129. Epub 2022 Mar 4.
5 Chemoproteomic profiling of kinases in live cells using electrophilic sulfonyl triazole probes. Chem Sci. 2021 Jan 21;12(9):3295-3307. doi: 10.1039/d0sc06623k.
6 Ynamide Electrophile for the Profiling of Ligandable Carboxyl Residues in Live Cells and the Development of New Covalent Inhibitors. J Med Chem. 2022 Aug 11;65(15):10408-10418. doi: 10.1021/acs.jmedchem.2c00272. Epub 2022 Jul 26.
7 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
8 A chemical proteomics approach for global mapping of functional lysines on cell surface of living cell. Nat Commun. 2024 Apr 8;15(1):2997. doi: 10.1038/s41467-024-47033-w.
Mass spectrometry data entry: PXD042888
9 An Azo Coupling-Based Chemoproteomic Approach to Systematically Profile the Tyrosine Reactivity in the Human Proteome. Anal Chem. 2021 Jul 27;93(29):10334-10342. doi: 10.1021/acs.analchem.1c01935. Epub 2021 Jul 12.
10 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
11 2H-Azirine-Based Reagents for Chemoselective Bioconjugation at Carboxyl Residues Inside Live Cells. J Am Chem Soc. 2020 Apr 1;142(13):6051-6059. doi: 10.1021/jacs.9b12116. Epub 2020 Mar 23.
12 Discovery of a Cell-Active SuTEx Ligand of Prostaglandin Reductase 2. Chembiochem. 2021 Jun 15;22(12):2134-2139. doi: 10.1002/cbic.202000879. Epub 2021 Apr 29.
13 Global targeting of functional tyrosines using sulfur-triazole exchange chemistry. Nat Chem Biol. 2020 Feb;16(2):150-159. doi: 10.1038/s41589-019-0404-5. Epub 2019 Nov 25.
14 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
15 Oxidant-Induced Bioconjugation for Protein Labeling in Live Cells. ACS Chem Biol. 2023 Jan 20;18(1):112-122. doi: 10.1021/acschembio.2c00740. Epub 2022 Dec 21.
16 Global profiling of functional histidines in live cells using small-molecule photosensitizer and chemical probe relay labelling. Nat Chem. 2024 Jun 4. doi: 10.1038/s41557-024-01545-6. Online ahead of print.
Mass spectrometry data entry: PXD042377
17 Targeted Proteomic Approaches for Proteome-Wide Characterizations of the AMP-Binding Capacities of Kinases. J Proteome Res. 2022 Aug 5;21(8):2063-2070. doi: 10.1021/acs.jproteome.2c00225. Epub 2022 Jul 12.
18 Comparison of Quantitative Mass Spectrometry Platforms for Monitoring Kinase ATP Probe Uptake in Lung Cancer. J Proteome Res. 2018 Jan 5;17(1):63-75. doi: 10.1021/acs.jproteome.7b00329. Epub 2017 Nov 22.
Mass spectrometry data entry: PXD006095 , PXD006096
19 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
20 Tunable Amine-Reactive Electrophiles for Selective Profiling of Lysine. Angew Chem Int Ed Engl. 2022 Jan 26;61(5):e202112107. doi: 10.1002/anie.202112107. Epub 2021 Dec 16.
21 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
22 Solid Phase Synthesis of Fluorosulfate Containing Macrocycles for Chemoproteomic Workflows. bioRxiv [Preprint]. 2023 Feb 18:2023.02.17.529022. doi: 10.1101/2023.02.17.529022.
Mass spectrometry data entry: PXD039931
23 DFT-Guided Discovery of Ethynyl-Triazolyl-Phosphinates as Modular Electrophiles for Chemoselective Cysteine Bioconjugation and Profiling. Angew Chem Int Ed Engl. 2022 Oct 10;61(41):e202205348. doi: 10.1002/anie.202205348. Epub 2022 Aug 22.
Mass spectrometry data entry: PXD033004
24 Oxidative cyclization reagents reveal tryptophan cation- interactions. Nature. 2024 Mar;627(8004):680-687. doi: 10.1038/s41586-024-07140-6. Epub 2024 Mar 6.
Mass spectrometry data entry: PXD001377 , PXD005252
25 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
26 Differently Tagged Probes for Protein Profiling of Mitochondria. Chembiochem. 2019 May 2;20(9):1155-1160. doi: 10.1002/cbic.201800735. Epub 2019 Mar 26.
27 Activity-based proteome profiling of potential cellular targets of Orlistat--an FDA-approved drug with anti-tumor activities. J Am Chem Soc. 2010 Jan 20;132(2):656-66. doi: 10.1021/ja907716f.
28 Design and synthesis of minimalist terminal alkyne-containing diazirine photo-crosslinkers and their incorporation into kinase inhibitors for cell- and tissue-based proteome profiling. Angew Chem Int Ed Engl. 2013 Aug 12;52(33):8551-6. doi: 10.1002/anie.201300683. Epub 2013 Jun 10.