Details of the Target

General Information of Target

Target ID LDTP01480
Target Name Calsyntenin-1 (CLSTN1)
Gene Name CLSTN1
Gene ID 22883
Synonyms
CS1; KIAA0911; Calsyntenin-1; Alcadein-alpha; Alc-alpha; Alzheimer-related cadherin-like protein; Non-classical cadherin XB31alpha) [Cleaved into: Soluble Alc-alpha; SAlc-alpha; CTF1-alpha; C-terminal fragment 1-alpha)]
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MLRRPAPALAPAARLLLAGLLCGGGVWAARVNKHKPWLEPTYHGIVTENDNTVLLDPPLI
ALDKDAPLRFAESFEVTVTKEGEICGFKIHGQNVPFDAVVVDKSTGEGVIRSKEKLDCEL
QKDYSFTIQAYDCGKGPDGTNVKKSHKATVHIQVNDVNEYAPVFKEKSYKATVIEGKQYD
SILRVEAVDADCSPQFSQICSYEIITPDVPFTVDKDGYIKNTEKLNYGKEHQYKLTVTAY
DCGKKRATEDVLVKISIKPTCTPGWQGWNNRIEYEPGTGALAVFPNIHLETCDEPVASVQ
ATVELETSHIGKGCDRDTYSEKSLHRLCGAAAGTAELLPSPSGSLNWTMGLPTDNGHDSD
QVFEFNGTQAVRIPDGVVSVSPKEPFTISVWMRHGPFGRKKETILCSSDKTDMNRHHYSL
YVHGCRLIFLFRQDPSEEKKYRPAEFHWKLNQVCDEEWHHYVLNVEFPSVTLYVDGTSHE
PFSVTEDYPLHPSKIETQLVVGACWQEFSGVENDNETEPVTVASAGGDLHMTQFFRGNLA
GLTLRSGKLADKKVIDCLYTCKEGLDLQVLEDSGRGVQIQAHPSQLVLTLEGEDLGELDK
AMQHISYLNSRQFPTPGIRRLKITSTIKCFNEATCISVPPVDGYVMVLQPEEPKISLSGV
HHFARAASEFESSEGVFLFPELRIISTITREVEPEGDGAEDPTVQESLVSEEIVHDLDTC
EVTVEGEELNHEQESLEVDMARLQQKGIEVSSSELGMTFTGVDTMASYEEVLHLLRYRNW
HARSLLDRKFKLICSELNGRYISNEFKVEVNVIHTANPMEHANHMAAQPQFVHPEHRSFV
DLSGHNLANPHPFAVVPSTATVVIVVCVSFLVFMIILGVFRIRAAHRRTMRDQDTGKENE
MDWDDSALTITVNPMETYEDQHSSEEEEEEEEEEESEDGEEEDDITSAESESSEEEEGEQ
GDPQNATRQQQLEWDDSTLSY
Target Bioclass
Other
Family
Calsyntenin family
Subcellular location
Nucleus; Postsynaptic cell membrane
Function
Postsynaptic adhesion molecule that binds to presynaptic neurexins to mediate both excitatory and inhibitory synapse formation. Promotes synapse development by acting as a cell adhesion molecule at the postsynaptic membrane, which associates with neurexin-alpha at the presynaptic membrane. Also functions as a cargo in axonal anterograde transport by acting as a molecular adapter that promotes KLC1 association with vesicles. Complex formation with APBA2 and APP, stabilizes APP metabolism and enhances APBA2-mediated suppression of beta-APP40 secretion, due to the retardation of intracellular APP maturation.; [Soluble Alc-alpha]: As intracellular fragment AlcICD, suppresses APBB1-dependent transactivation stimulated by APP C-terminal intracellular fragment (AICD), most probably by competing with AICD for APBB1-binding.; [CTF1-alpha]: In complex with APBA2 and C99, a C-terminal APP fragment, abolishes C99 interaction with PSEN1 and thus APP C99 cleavage by gamma-secretase, most probably through stabilization of the direct interaction between APBA2 and APP.
Uniprot ID
O94985
Ensemble ID
ENST00000361311.4
HGNC ID
HGNC:17447

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
CCK81 SNV: p.F96L .
CMK SNV: p.H151L .
G415 SNV: p.R665L .
GAMG SNV: p.A6T; p.A13V .
HEC1B SNV: p.T634I .
MEWO SNV: p.H662Y DBIA    Probe Info 
RKO SNV: p.C22R .
SNGM SNV: p.P650H .
SNU478 SNV: p.S381R DBIA    Probe Info 
TOV21G SNV: p.P207T .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 3 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
OPA-S-S-alkyne
 Probe Info  		K322(0.81)  LDD3494  [1]
DBIA
 Probe Info  		C242(0.97)  LDD0547  [2]
IA-alkyne
 Probe Info  		N.A.  LDD0165  [3]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0230  AC113 HCT 116 C242(0.97)  LDD0547  [2]
 LDCM0231  AC114 HCT 116 C242(0.78)  LDD0548  [2]
 LDCM0232  AC115 HCT 116 C242(0.84)  LDD0549  [2]
 LDCM0233  AC116 HCT 116 C242(0.94)  LDD0550  [2]
 LDCM0234  AC117 HCT 116 C242(0.93)  LDD0551  [2]
 LDCM0235  AC118 HCT 116 C242(0.80)  LDD0552  [2]
 LDCM0236  AC119 HCT 116 C242(0.92)  LDD0553  [2]
 LDCM0238  AC120 HCT 116 C242(1.06)  LDD0555  [2]
 LDCM0239  AC121 HCT 116 C242(0.86)  LDD0556  [2]
 LDCM0240  AC122 HCT 116 C242(0.98)  LDD0557  [2]
 LDCM0241  AC123 HCT 116 C242(0.82)  LDD0558  [2]
 LDCM0242  AC124 HCT 116 C242(1.00)  LDD0559  [2]
 LDCM0243  AC125 HCT 116 C242(0.94)  LDD0560  [2]
 LDCM0244  AC126 HCT 116 C242(0.84)  LDD0561  [2]
 LDCM0245  AC127 HCT 116 C242(0.95)  LDD0562  [2]
 LDCM0246  AC128 PaTu 8988t C242(1.15)  LDD1125  [2]
 LDCM0247  AC129 PaTu 8988t C242(1.21)  LDD1126  [2]
 LDCM0249  AC130 PaTu 8988t C242(1.08)  LDD1128  [2]
 LDCM0250  AC131 PaTu 8988t C242(1.27)  LDD1129  [2]
 LDCM0251  AC132 PaTu 8988t C242(0.99)  LDD1130  [2]
 LDCM0252  AC133 PaTu 8988t C242(1.45)  LDD1131  [2]
 LDCM0253  AC134 PaTu 8988t C242(1.33)  LDD1132  [2]
 LDCM0254  AC135 PaTu 8988t C242(1.23)  LDD1133  [2]
 LDCM0255  AC136 PaTu 8988t C242(1.27)  LDD1134  [2]
 LDCM0256  AC137 PaTu 8988t C242(1.31)  LDD1135  [2]
 LDCM0257  AC138 PaTu 8988t C242(1.04)  LDD1136  [2]
 LDCM0258  AC139 PaTu 8988t C242(1.10)  LDD1137  [2]
 LDCM0260  AC140 PaTu 8988t C242(1.09)  LDD1139  [2]
 LDCM0261  AC141 PaTu 8988t C242(1.11)  LDD1140  [2]
 LDCM0262  AC142 PaTu 8988t C242(1.14)  LDD1141  [2]
 LDCM0263  AC143 PaTu 8988t C242(1.03)  LDD1142  [2]
 LDCM0264  AC144 PaTu 8988t C242(1.09)  LDD1143  [2]
 LDCM0265  AC145 PaTu 8988t C242(0.99)  LDD1144  [2]
 LDCM0266  AC146 PaTu 8988t C242(1.24)  LDD1145  [2]
 LDCM0267  AC147 PaTu 8988t C242(0.97)  LDD1146  [2]
 LDCM0268  AC148 PaTu 8988t C242(1.13)  LDD1147  [2]
 LDCM0269  AC149 PaTu 8988t C242(1.25)  LDD1148  [2]
 LDCM0271  AC150 PaTu 8988t C242(1.24)  LDD1150  [2]
 LDCM0272  AC151 PaTu 8988t C242(1.52)  LDD1151  [2]
 LDCM0273  AC152 PaTu 8988t C242(1.20)  LDD1152  [2]
 LDCM0274  AC153 PaTu 8988t C242(1.15)  LDD1153  [2]
 LDCM0621  AC154 PaTu 8988t C242(1.45)  LDD2166  [2]
 LDCM0622  AC155 PaTu 8988t C242(1.38)  LDD2167  [2]
 LDCM0623  AC156 PaTu 8988t C242(1.20)  LDD2168  [2]
 LDCM0624  AC157 PaTu 8988t C242(1.22)  LDD2169  [2]
 LDCM0296  AC35 HCT 116 C242(1.01)  LDD0613  [2]
 LDCM0297  AC36 HCT 116 C242(0.94)  LDD0614  [2]
 LDCM0298  AC37 HCT 116 C242(0.84)  LDD0615  [2]
 LDCM0299  AC38 HCT 116 C242(1.08)  LDD0616  [2]
 LDCM0300  AC39 HCT 116 C242(1.05)  LDD0617  [2]
 LDCM0302  AC40 HCT 116 C242(0.95)  LDD0619  [2]
 LDCM0303  AC41 HCT 116 C242(0.94)  LDD0620  [2]
 LDCM0304  AC42 HCT 116 C242(1.07)  LDD0621  [2]
 LDCM0305  AC43 HCT 116 C242(0.96)  LDD0622  [2]
 LDCM0306  AC44 HCT 116 C242(0.96)  LDD0623  [2]
 LDCM0307  AC45 HCT 116 C242(0.98)  LDD0624  [2]
 LDCM0320  AC57 PaTu 8988t C242(1.04)  LDD1199  [2]
 LDCM0321  AC58 PaTu 8988t C242(0.88)  LDD1200  [2]
 LDCM0322  AC59 PaTu 8988t C242(1.01)  LDD1201  [2]
 LDCM0324  AC60 PaTu 8988t C242(0.98)  LDD1203  [2]
 LDCM0325  AC61 PaTu 8988t C242(0.92)  LDD1204  [2]
 LDCM0326  AC62 PaTu 8988t C242(0.96)  LDD1205  [2]
 LDCM0327  AC63 PaTu 8988t C242(0.91)  LDD1206  [2]
 LDCM0328  AC64 PaTu 8988t C242(1.04)  LDD1207  [2]
 LDCM0329  AC65 PaTu 8988t C242(1.04)  LDD1208  [2]
 LDCM0330  AC66 PaTu 8988t C242(0.87)  LDD1209  [2]
 LDCM0331  AC67 PaTu 8988t C242(1.01)  LDD1210  [2]
 LDCM0332  AC68 PaTu 8988t C242(1.14)  LDD1211  [2]
 LDCM0333  AC69 PaTu 8988t C242(1.15)  LDD1212  [2]
 LDCM0335  AC70 PaTu 8988t C242(1.12)  LDD1214  [2]
 LDCM0336  AC71 PaTu 8988t C242(1.03)  LDD1215  [2]
 LDCM0337  AC72 PaTu 8988t C242(1.05)  LDD1216  [2]
 LDCM0338  AC73 PaTu 8988t C242(1.17)  LDD1217  [2]
 LDCM0339  AC74 PaTu 8988t C242(1.08)  LDD1218  [2]
 LDCM0340  AC75 PaTu 8988t C242(1.25)  LDD1219  [2]
 LDCM0341  AC76 PaTu 8988t C242(1.22)  LDD1220  [2]
 LDCM0342  AC77 PaTu 8988t C242(1.08)  LDD1221  [2]
 LDCM0343  AC78 PaTu 8988t C242(1.15)  LDD1222  [2]
 LDCM0344  AC79 PaTu 8988t C242(1.14)  LDD1223  [2]
 LDCM0346  AC80 PaTu 8988t C242(1.34)  LDD1225  [2]
 LDCM0347  AC81 PaTu 8988t C242(1.23)  LDD1226  [2]
 LDCM0348  AC82 PaTu 8988t C242(1.19)  LDD1227  [2]
 LDCM0367  CL1 PaTu 8988t C242(1.31)  LDD1246  [2]
 LDCM0368  CL10 PaTu 8988t C242(2.23)  LDD1247  [2]
 LDCM0379  CL11 PaTu 8988t C242(1.64)  LDD1258  [2]
 LDCM0387  CL117 HCT 116 C242(1.01)  LDD0704  [2]
 LDCM0388  CL118 HCT 116 C242(1.00)  LDD0705  [2]
 LDCM0389  CL119 HCT 116 C242(1.05)  LDD0706  [2]
 LDCM0390  CL12 PaTu 8988t C242(1.42)  LDD1269  [2]
 LDCM0391  CL120 HCT 116 C242(0.89)  LDD0708  [2]
 LDCM0396  CL125 PaTu 8988t C242(0.93)  LDD1275  [2]
 LDCM0397  CL126 PaTu 8988t C242(1.01)  LDD1276  [2]
 LDCM0398  CL127 PaTu 8988t C242(0.99)  LDD1277  [2]
 LDCM0399  CL128 PaTu 8988t C242(1.04)  LDD1278  [2]
 LDCM0400  CL13 PaTu 8988t C242(1.97)  LDD1279  [2]
 LDCM0401  CL14 PaTu 8988t C242(1.43)  LDD1280  [2]
 LDCM0402  CL15 PaTu 8988t C242(1.64)  LDD1281  [2]
 LDCM0407  CL2 PaTu 8988t C242(0.98)  LDD1286  [2]
 LDCM0418  CL3 PaTu 8988t C242(1.12)  LDD1297  [2]
 LDCM0420  CL31 HCT 116 C242(1.19)  LDD0737  [2]
 LDCM0421  CL32 HCT 116 C242(0.97)  LDD0738  [2]
 LDCM0422  CL33 HCT 116 C242(0.99)  LDD0739  [2]
 LDCM0423  CL34 HCT 116 C242(0.99)  LDD0740  [2]
 LDCM0424  CL35 HCT 116 C242(1.05)  LDD0741  [2]
 LDCM0425  CL36 HCT 116 C242(1.10)  LDD0742  [2]
 LDCM0426  CL37 HCT 116 C242(0.99)  LDD0743  [2]
 LDCM0428  CL39 HCT 116 C242(1.06)  LDD0745  [2]
 LDCM0429  CL4 PaTu 8988t C242(1.47)  LDD1308  [2]
 LDCM0430  CL40 HCT 116 C242(1.21)  LDD0747  [2]
 LDCM0431  CL41 HCT 116 C242(1.04)  LDD0748  [2]
 LDCM0432  CL42 HCT 116 C242(0.95)  LDD0749  [2]
 LDCM0433  CL43 HCT 116 C242(1.10)  LDD0750  [2]
 LDCM0434  CL44 HCT 116 C242(1.28)  LDD0751  [2]
 LDCM0435  CL45 HCT 116 C242(1.30)  LDD0752  [2]
 LDCM0436  CL46 HCT 116 C242(1.09)  LDD0753  [2]
 LDCM0437  CL47 HCT 116 C242(0.99)  LDD0754  [2]
 LDCM0438  CL48 HCT 116 C242(0.95)  LDD0755  [2]
 LDCM0439  CL49 HCT 116 C242(0.97)  LDD0756  [2]
 LDCM0440  CL5 PaTu 8988t C242(1.06)  LDD1319  [2]
 LDCM0441  CL50 HCT 116 C242(1.02)  LDD0758  [2]
 LDCM0442  CL51 HCT 116 C242(1.06)  LDD0759  [2]
 LDCM0443  CL52 HCT 116 C242(1.11)  LDD0760  [2]
 LDCM0444  CL53 HCT 116 C242(0.94)  LDD0761  [2]
 LDCM0445  CL54 HCT 116 C242(1.02)  LDD0762  [2]
 LDCM0446  CL55 HCT 116 C242(0.96)  LDD0763  [2]
 LDCM0447  CL56 HCT 116 C242(0.95)  LDD0764  [2]
 LDCM0448  CL57 HCT 116 C242(1.08)  LDD0765  [2]
 LDCM0449  CL58 HCT 116 C242(0.93)  LDD0766  [2]
 LDCM0450  CL59 HCT 116 C242(1.03)  LDD0767  [2]
 LDCM0451  CL6 PaTu 8988t C242(1.17)  LDD1330  [2]
 LDCM0452  CL60 HCT 116 C242(1.07)  LDD0769  [2]
 LDCM0453  CL61 HCT 116 C242(1.03)  LDD0770  [2]
 LDCM0454  CL62 HCT 116 C242(0.98)  LDD0771  [2]
 LDCM0455  CL63 HCT 116 C242(1.05)  LDD0772  [2]
 LDCM0456  CL64 HCT 116 C242(1.06)  LDD0773  [2]
 LDCM0457  CL65 HCT 116 C242(1.07)  LDD0774  [2]
 LDCM0458  CL66 HCT 116 C242(1.12)  LDD0775  [2]
 LDCM0459  CL67 HCT 116 C242(0.99)  LDD0776  [2]
 LDCM0460  CL68 HCT 116 C242(1.08)  LDD0777  [2]
 LDCM0461  CL69 HCT 116 C242(1.27)  LDD0778  [2]
 LDCM0462  CL7 PaTu 8988t C242(1.24)  LDD1341  [2]
 LDCM0463  CL70 HCT 116 C242(0.96)  LDD0780  [2]
 LDCM0464  CL71 HCT 116 C242(1.11)  LDD0781  [2]
 LDCM0465  CL72 HCT 116 C242(1.38)  LDD0782  [2]
 LDCM0466  CL73 HCT 116 C242(1.07)  LDD0783  [2]
 LDCM0467  CL74 HCT 116 C242(1.14)  LDD0784  [2]
 LDCM0469  CL76 PaTu 8988t C242(1.24)  LDD1348  [2]
 LDCM0470  CL77 PaTu 8988t C242(1.03)  LDD1349  [2]
 LDCM0471  CL78 PaTu 8988t C242(1.32)  LDD1350  [2]
 LDCM0472  CL79 PaTu 8988t C242(1.18)  LDD1351  [2]
 LDCM0473  CL8 PaTu 8988t C242(1.06)  LDD1352  [2]
 LDCM0474  CL80 PaTu 8988t C242(1.31)  LDD1353  [2]
 LDCM0475  CL81 PaTu 8988t C242(1.32)  LDD1354  [2]
 LDCM0476  CL82 PaTu 8988t C242(1.36)  LDD1355  [2]
 LDCM0477  CL83 PaTu 8988t C242(1.33)  LDD1356  [2]
 LDCM0478  CL84 PaTu 8988t C242(1.46)  LDD1357  [2]
 LDCM0479  CL85 PaTu 8988t C242(1.03)  LDD1358  [2]
 LDCM0480  CL86 PaTu 8988t C242(1.35)  LDD1359  [2]
 LDCM0481  CL87 PaTu 8988t C242(1.53)  LDD1360  [2]
 LDCM0482  CL88 PaTu 8988t C242(1.45)  LDD1361  [2]
 LDCM0483  CL89 PaTu 8988t C242(1.38)  LDD1362  [2]
 LDCM0484  CL9 PaTu 8988t C242(1.26)  LDD1363  [2]
 LDCM0485  CL90 PaTu 8988t C242(1.57)  LDD1364  [2]
 LDCM0468  Fragment33 HCT 116 C242(1.03)  LDD0785  [2]
 LDCM0427  Fragment51 HCT 116 C242(0.87)  LDD0744  [2]
 LDCM0022  KB02 HEK-293T C118(0.96); C406(0.99); C242(0.95); C261(0.91)  LDD1492  [4]
 LDCM0023  KB03 HEK-293T C118(1.01); C406(0.98); C242(1.07); C261(1.11)  LDD1497  [4]
 LDCM0024  KB05 MKN45 C261(2.62)  LDD3332  [5]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
E3 ubiquitin-protein ligase RNF10 (RNF10) RNF10 family Q8N5U6
E3 ubiquitin-protein ligase PPP1R11 (PPP1R11) . O60927
Transporter and channel
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Amyloid-beta precursor protein (APP) APP family P05067
Amyloid-beta A4 precursor protein-binding family A member 2 (APBA2) . Q99767
Other
Click To Hide/Show 5 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
SNW domain-containing protein 1 (SNW1) SNW family Q13573
Histone-binding protein RBBP4 (RBBP4) WD repeat RBAP46/RBAP48/MSI1 family Q09028
BTB/POZ domain-containing protein KCTD17 (KCTD17) . Q8N5Z5
Numb-like protein (NUMBL) . Q9Y6R0
Putative apolipoprotein(a)-like protein 2 (LPAL2) . Q16609

References

1 A chemical proteomics approach for global mapping of functional lysines on cell surface of living cell. Nat Commun. 2024 Apr 8;15(1):2997. doi: 10.1038/s41467-024-47033-w.
Mass spectrometry data entry: PXD042888
2 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
3 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
4 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
5 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840