Details of the Target

General Information of Target

Target ID LDTP01165
Target Name NADH dehydrogenase iron-sulfur protein 7, mitochondrial (NDUFS7)
Gene Name NDUFS7
Gene ID 374291
Synonyms
NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial; EC 7.1.1.2; Complex I-20kD; CI-20kD; NADH-ubiquinone oxidoreductase 20 kDa subunit; PSST subunit
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
Download
Sequence
MAVLSAPGLRGFRILGLRSSVGPAVQARGVHQSVATDGPSSTQPALPKARAVAPKPSSRG
EYVVAKLDDLVNWARRSSLWPMTFGLACCAVEMMHMAAPRYDMDRFGVVFRASPRQSDVM
IVAGTLTNKMAPALRKVYDQMPEPRYVVSMGSCANGGGYYHYSYSVVRGCDRIVPVDIYI
PGCPPTAEALLYGILQLQRKIKRERRLQIWYRR
Target Bioclass
Enzyme
Family
Complex I 20 kDa subunit family
Subcellular location
Mitochondrion inner membrane
Function
Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which catalyzes electron transfer from NADH through the respiratory chain, using ubiquinone as an electron acceptor. Essential for the catalytic activity of complex I.
Uniprot ID
O75251
Ensemble ID
ENST00000233627.14
HGNC ID
HGNC:7714
ChEMBL ID
CHEMBL2363065

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
CCK81 SNV: p.G193S .
COLO320 SNV: p.C183W .
IM95 SNV: p.G193S .
JURKAT SNV: p.A154T .
MFE319 SNV: p.Y162H .
P31FUJ SNV: p.T42P .
SHP77 Substitution: p.R76L .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 20 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
TH211
 Probe Info  		Y62(16.45)  LDD0257  [1]
ONAyne
 Probe Info  		N.A.  LDD0273  [2]
Probe 1
 Probe Info  		Y62(23.80)  LDD3495  [3]
DBIA
 Probe Info  		C183(1.25)  LDD3342  [4]
Jackson_14
 Probe Info  		5.88  LDD0123  [5]
IPM
 Probe Info  		C194(4.56)  LDD0406  [6]
1d-yne
 Probe Info  		N.A.  LDD0358  [7]
4-Iodoacetamidophenylacetylene
 Probe Info  		N.A.  LDD0038  [8]
IA-alkyne
 Probe Info  		C183(0.00); C88(0.00); C89(0.00)  LDD0036  [8]
Lodoacetamide azide
 Probe Info  		N.A.  LDD0037  [8]
NAIA_4
 Probe Info  		N.A.  LDD2226  [9]
NHS
 Probe Info  		N.A.  LDD0010  [10]
STPyne
 Probe Info  		N.A.  LDD0009  [10]
Ox-W18
 Probe Info  		N.A.  LDD2175  [11]
1c-yne
 Probe Info  		K136(0.00); K66(0.00)  LDD0228  [7]
Acrolein
 Probe Info  		N.A.  LDD0217  [12]
AOyne
 Probe Info  		11.10  LDD0443  [13]
NAIA_5
 Probe Info  		C183(0.00); C153(0.00)  LDD2223  [9]
TER-AC
 Probe Info  		N.A.  LDD0426  [14]
TPP-AC
 Probe Info  		N.A.  LDD0427  [14]
PAL-AfBPP Probe
Click To Hide/Show 6 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
FFF probe11
 Probe Info  		20.00  LDD0471  [15]
FFF probe14
 Probe Info  		20.00  LDD0477  [15]
FFF probe2
 Probe Info  		18.94  LDD0463  [15]
FFF probe3
 Probe Info  		13.81  LDD0464  [15]
JN0003
 Probe Info  		20.00  LDD0469  [15]
OEA-DA
 Probe Info  		3.09  LDD0046  [16]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0214  AC1 HEK-293T C183(1.00)  LDD1507  [17]
 LDCM0226  AC11 HEK-293T C183(0.90)  LDD1509  [17]
 LDCM0259  AC14 HEK-293T C183(1.01)  LDD1512  [17]
 LDCM0270  AC15 HEK-293T C183(0.91); C88(0.85)  LDD1513  [17]
 LDCM0276  AC17 HEK-293T C183(1.14)  LDD1515  [17]
 LDCM0278  AC19 HEK-293T C183(0.88)  LDD1517  [17]
 LDCM0281  AC21 HEK-293T C183(0.87)  LDD1520  [17]
 LDCM0282  AC22 HEK-293T C183(1.04)  LDD1521  [17]
 LDCM0283  AC23 HEK-293T C183(0.99); C88(0.75)  LDD1522  [17]
 LDCM0284  AC24 HEK-293T C183(0.97)  LDD1523  [17]
 LDCM0285  AC25 HEK-293T C183(0.97)  LDD1524  [17]
 LDCM0287  AC27 HEK-293T C183(0.82)  LDD1526  [17]
 LDCM0289  AC29 HEK-293T C183(0.89)  LDD1528  [17]
 LDCM0290  AC3 HEK-293T C183(0.98)  LDD1529  [17]
 LDCM0291  AC30 HEK-293T C183(0.80)  LDD1530  [17]
 LDCM0292  AC31 HEK-293T C183(0.95); C88(0.87)  LDD1531  [17]
 LDCM0293  AC32 HEK-293T C183(0.96)  LDD1532  [17]
 LDCM0294  AC33 HEK-293T C183(1.03)  LDD1533  [17]
 LDCM0296  AC35 HEK-293T C183(0.92)  LDD1535  [17]
 LDCM0298  AC37 HEK-293T C183(0.91)  LDD1537  [17]
 LDCM0299  AC38 HEK-293T C183(0.93)  LDD1538  [17]
 LDCM0300  AC39 HEK-293T C183(0.99); C88(0.90)  LDD1539  [17]
 LDCM0302  AC40 HEK-293T C183(1.21)  LDD1541  [17]
 LDCM0303  AC41 HEK-293T C183(1.01)  LDD1542  [17]
 LDCM0305  AC43 HEK-293T C183(0.96)  LDD1544  [17]
 LDCM0307  AC45 HEK-293T C183(0.82)  LDD1546  [17]
 LDCM0308  AC46 HEK-293T C183(1.12)  LDD1547  [17]
 LDCM0309  AC47 HEK-293T C183(0.97); C88(1.08)  LDD1548  [17]
 LDCM0310  AC48 HEK-293T C183(1.28)  LDD1549  [17]
 LDCM0311  AC49 HEK-293T C183(0.93)  LDD1550  [17]
 LDCM0312  AC5 HEK-293T C183(0.91)  LDD1551  [17]
 LDCM0314  AC51 HEK-293T C183(0.92)  LDD1553  [17]
 LDCM0316  AC53 HEK-293T C183(0.80)  LDD1555  [17]
 LDCM0317  AC54 HEK-293T C183(1.35)  LDD1556  [17]
 LDCM0318  AC55 HEK-293T C183(0.95); C88(0.91)  LDD1557  [17]
 LDCM0319  AC56 HEK-293T C183(0.83)  LDD1558  [17]
 LDCM0320  AC57 HEK-293T C183(1.48)  LDD1559  [17]
 LDCM0322  AC59 HEK-293T C183(1.01)  LDD1561  [17]
 LDCM0323  AC6 HEK-293T C183(1.01)  LDD1562  [17]
 LDCM0325  AC61 HEK-293T C183(0.98)  LDD1564  [17]
 LDCM0326  AC62 HEK-293T C183(0.94)  LDD1565  [17]
 LDCM0327  AC63 HEK-293T C183(1.10); C88(0.87)  LDD1566  [17]
 LDCM0328  AC64 HEK-293T C183(1.24)  LDD1567  [17]
 LDCM0334  AC7 HEK-293T C183(1.06); C88(0.87)  LDD1568  [17]
 LDCM0345  AC8 HEK-293T C183(1.03)  LDD1569  [17]
 LDCM0248  AKOS034007472 HEK-293T C183(0.99)  LDD1511  [17]
 LDCM0356  AKOS034007680 HEK-293T C183(0.87)  LDD1570  [17]
 LDCM0275  AKOS034007705 HEK-293T C183(1.00)  LDD1514  [17]
 LDCM0630  CCW28-3 231MFP C183(1.14)  LDD2214  [18]
 LDCM0367  CL1 HEK-293T C183(1.30)  LDD1571  [17]
 LDCM0368  CL10 HEK-293T C183(0.91)  LDD1572  [17]
 LDCM0369  CL100 HEK-293T C183(0.93)  LDD1573  [17]
 LDCM0370  CL101 HEK-293T C183(1.07)  LDD1574  [17]
 LDCM0371  CL102 HEK-293T C183(1.29)  LDD1575  [17]
 LDCM0372  CL103 HEK-293T C183(1.65)  LDD1576  [17]
 LDCM0373  CL104 HEK-293T C183(1.00)  LDD1577  [17]
 LDCM0374  CL105 HEK-293T C183(0.93)  LDD1578  [17]
 LDCM0375  CL106 HEK-293T C183(0.95)  LDD1579  [17]
 LDCM0376  CL107 HEK-293T C183(0.95)  LDD1580  [17]
 LDCM0377  CL108 HEK-293T C183(1.13)  LDD1581  [17]
 LDCM0378  CL109 HEK-293T C183(0.96)  LDD1582  [17]
 LDCM0379  CL11 HEK-293T C183(1.21); C88(0.64)  LDD1583  [17]
 LDCM0380  CL110 HEK-293T C183(1.01)  LDD1584  [17]
 LDCM0381  CL111 HEK-293T C183(0.63)  LDD1585  [17]
 LDCM0382  CL112 HEK-293T C183(1.04)  LDD1586  [17]
 LDCM0383  CL113 HEK-293T C183(1.35)  LDD1587  [17]
 LDCM0384  CL114 HEK-293T C183(0.90)  LDD1588  [17]
 LDCM0385  CL115 HEK-293T C183(0.78)  LDD1589  [17]
 LDCM0386  CL116 HEK-293T C183(1.02)  LDD1590  [17]
 LDCM0387  CL117 HEK-293T C183(1.10)  LDD1591  [17]
 LDCM0388  CL118 HEK-293T C183(0.84)  LDD1592  [17]
 LDCM0389  CL119 HEK-293T C183(0.90)  LDD1593  [17]
 LDCM0390  CL12 HEK-293T C183(1.31)  LDD1594  [17]
 LDCM0391  CL120 HEK-293T C183(1.04)  LDD1595  [17]
 LDCM0392  CL121 HEK-293T C183(0.94)  LDD1596  [17]
 LDCM0393  CL122 HEK-293T C183(1.42)  LDD1597  [17]
 LDCM0394  CL123 HEK-293T C183(1.11)  LDD1598  [17]
 LDCM0395  CL124 HEK-293T C183(0.97)  LDD1599  [17]
 LDCM0396  CL125 HEK-293T C183(1.05)  LDD1600  [17]
 LDCM0397  CL126 HEK-293T C183(1.07)  LDD1601  [17]
 LDCM0398  CL127 HEK-293T C183(0.98)  LDD1602  [17]
 LDCM0399  CL128 HEK-293T C183(1.24)  LDD1603  [17]
 LDCM0400  CL13 HEK-293T C183(0.95)  LDD1604  [17]
 LDCM0401  CL14 HEK-293T C183(1.33)  LDD1605  [17]
 LDCM0402  CL15 HEK-293T C183(2.04)  LDD1606  [17]
 LDCM0403  CL16 HEK-293T C183(1.67)  LDD1607  [17]
 LDCM0404  CL17 HEK-293T C183(2.69)  LDD1608  [17]
 LDCM0406  CL19 HEK-293T C183(1.07)  LDD1610  [17]
 LDCM0407  CL2 HEK-293T C183(1.47)  LDD1611  [17]
 LDCM0409  CL21 HEK-293T C183(0.57)  LDD1613  [17]
 LDCM0410  CL22 HEK-293T C183(1.12)  LDD1614  [17]
 LDCM0411  CL23 HEK-293T C183(0.94); C88(0.76)  LDD1615  [17]
 LDCM0412  CL24 HEK-293T C183(1.23)  LDD1616  [17]
 LDCM0413  CL25 HEK-293T C183(0.99)  LDD1617  [17]
 LDCM0414  CL26 HEK-293T C183(1.65)  LDD1618  [17]
 LDCM0415  CL27 HEK-293T C183(0.75)  LDD1619  [17]
 LDCM0416  CL28 HEK-293T C183(1.21)  LDD1620  [17]
 LDCM0417  CL29 HEK-293T C183(0.96)  LDD1621  [17]
 LDCM0418  CL3 HEK-293T C183(1.40)  LDD1622  [17]
 LDCM0420  CL31 HEK-293T C183(0.85)  LDD1624  [17]
 LDCM0422  CL33 HEK-293T C183(0.95)  LDD1626  [17]
 LDCM0423  CL34 HEK-293T C183(0.97)  LDD1627  [17]
 LDCM0424  CL35 HEK-293T C183(1.47); C88(0.61)  LDD1628  [17]
 LDCM0425  CL36 HEK-293T C183(1.18)  LDD1629  [17]
 LDCM0426  CL37 HEK-293T C183(1.14)  LDD1630  [17]
 LDCM0428  CL39 HEK-293T C183(1.03)  LDD1632  [17]
 LDCM0429  CL4 HEK-293T C183(1.00)  LDD1633  [17]
 LDCM0430  CL40 HEK-293T C183(1.02)  LDD1634  [17]
 LDCM0431  CL41 HEK-293T C183(0.79)  LDD1635  [17]
 LDCM0433  CL43 HEK-293T C183(0.87)  LDD1637  [17]
 LDCM0435  CL45 HEK-293T C183(1.05)  LDD1639  [17]
 LDCM0436  CL46 HEK-293T C183(0.91)  LDD1640  [17]
 LDCM0437  CL47 HEK-293T C183(1.15); C88(0.74)  LDD1641  [17]
 LDCM0438  CL48 HEK-293T C183(1.40)  LDD1642  [17]
 LDCM0439  CL49 HEK-293T C183(1.22)  LDD1643  [17]
 LDCM0440  CL5 HEK-293T C183(1.67)  LDD1644  [17]
 LDCM0441  CL50 HEK-293T C183(1.44)  LDD1645  [17]
 LDCM0443  CL52 HEK-293T C183(0.97)  LDD1646  [17]
 LDCM0444  CL53 HEK-293T C183(1.23)  LDD1647  [17]
 LDCM0446  CL55 HEK-293T C183(1.04)  LDD1649  [17]
 LDCM0448  CL57 HEK-293T C183(0.79)  LDD1651  [17]
 LDCM0449  CL58 HEK-293T C183(1.16)  LDD1652  [17]
 LDCM0450  CL59 HEK-293T C183(1.00); C88(0.81)  LDD1653  [17]
 LDCM0452  CL60 HEK-293T C183(1.34)  LDD1655  [17]
 LDCM0453  CL61 HEK-293T C183(1.16)  LDD1656  [17]
 LDCM0454  CL62 HEK-293T C183(1.31)  LDD1657  [17]
 LDCM0455  CL63 HEK-293T C183(1.25)  LDD1658  [17]
 LDCM0456  CL64 HEK-293T C183(1.34)  LDD1659  [17]
 LDCM0457  CL65 HEK-293T C183(1.14)  LDD1660  [17]
 LDCM0459  CL67 HEK-293T C183(1.06)  LDD1662  [17]
 LDCM0461  CL69 HEK-293T C183(1.06)  LDD1664  [17]
 LDCM0462  CL7 HEK-293T C183(0.97)  LDD1665  [17]
 LDCM0463  CL70 HEK-293T C183(1.09)  LDD1666  [17]
 LDCM0464  CL71 HEK-293T C183(0.97); C88(0.65)  LDD1667  [17]
 LDCM0465  CL72 HEK-293T C183(1.51)  LDD1668  [17]
 LDCM0466  CL73 HEK-293T C183(1.09)  LDD1669  [17]
 LDCM0467  CL74 HEK-293T C183(1.05)  LDD1670  [17]
 LDCM0469  CL76 HEK-293T C183(1.15)  LDD1672  [17]
 LDCM0470  CL77 HEK-293T C183(1.06)  LDD1673  [17]
 LDCM0472  CL79 HEK-293T C183(0.86)  LDD1675  [17]
 LDCM0475  CL81 HEK-293T C183(1.05)  LDD1678  [17]
 LDCM0476  CL82 HEK-293T C183(1.00)  LDD1679  [17]
 LDCM0477  CL83 HEK-293T C183(1.09); C88(0.72)  LDD1680  [17]
 LDCM0478  CL84 HEK-293T C183(1.16)  LDD1681  [17]
 LDCM0479  CL85 HEK-293T C183(0.97)  LDD1682  [17]
 LDCM0480  CL86 HEK-293T C183(0.90)  LDD1683  [17]
 LDCM0481  CL87 HEK-293T C183(0.83)  LDD1684  [17]
 LDCM0482  CL88 HEK-293T C183(1.29)  LDD1685  [17]
 LDCM0483  CL89 HEK-293T C183(1.10)  LDD1686  [17]
 LDCM0484  CL9 HEK-293T C183(0.98)  LDD1687  [17]
 LDCM0486  CL91 HEK-293T C183(0.94)  LDD1689  [17]
 LDCM0488  CL93 HEK-293T C183(0.86)  LDD1691  [17]
 LDCM0489  CL94 HEK-293T C183(0.96)  LDD1692  [17]
 LDCM0490  CL95 HEK-293T C183(1.15); C88(0.93)  LDD1693  [17]
 LDCM0491  CL96 HEK-293T C183(1.24)  LDD1694  [17]
 LDCM0492  CL97 HEK-293T C183(1.27)  LDD1695  [17]
 LDCM0493  CL98 HEK-293T C183(1.59)  LDD1696  [17]
 LDCM0494  CL99 HEK-293T C183(0.92)  LDD1697  [17]
 LDCM0495  E2913 HEK-293T C183(1.07)  LDD1698  [17]
 LDCM0157  EN6 HEK293-A C194(4.56)  LDD0406  [6]
 LDCM0468  Fragment33 HEK-293T C183(1.15)  LDD1671  [17]
 LDCM0427  Fragment51 HEK-293T C183(0.67)  LDD1631  [17]
 LDCM0022  KB02 AN3-CA C183(1.05)  LDD2264  [4]
 LDCM0023  KB03 AN3-CA C183(0.97)  LDD2681  [4]
 LDCM0024  KB05 NCI-H1155 C183(1.25)  LDD3342  [4]
 LDCM0109  NEM HeLa N.A.  LDD0224  [12]
 LDCM0016  Ranjitkar_cp1 MDA-MB-231 5.88  LDD0123  [5]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Transporter and channel
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Junctophilin-3 (JPH3) Junctophilin family Q8WXH2

The Drug(s) Related To This Target

Approved
Click To Hide/Show 2 Drug(s) Interacting with This Target
Drug Name Drug Type External ID
Doxorubicin Small molecular drug DB00997
Nadh Small molecular drug DB00157

References

1 Chemoproteomic profiling of kinases in live cells using electrophilic sulfonyl triazole probes. Chem Sci. 2021 Jan 21;12(9):3295-3307. doi: 10.1039/d0sc06623k.
2 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
3 An Azo Coupling-Based Chemoproteomic Approach to Systematically Profile the Tyrosine Reactivity in the Human Proteome. Anal Chem. 2021 Jul 27;93(29):10334-10342. doi: 10.1021/acs.analchem.1c01935. Epub 2021 Jul 12.
4 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
5 Appendage and Scaffold Diverse Fully Functionalized Small-Molecule Probes via a Minimalist Terminal Alkyne-Aliphatic Diazirine Isocyanide. J Org Chem. 2018 Sep 21;83(18):11245-11253. doi: 10.1021/acs.joc.8b01831. Epub 2018 Aug 31.
6 Covalent targeting of the vacuolar H(+)-ATPase activates autophagy via mTORC1 inhibition. Nat Chem Biol. 2019 Aug;15(8):776-785. doi: 10.1038/s41589-019-0308-4. Epub 2019 Jul 8.
7 Tunable Amine-Reactive Electrophiles for Selective Profiling of Lysine. Angew Chem Int Ed Engl. 2022 Jan 26;61(5):e202112107. doi: 10.1002/anie.202112107. Epub 2021 Dec 16.
8 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
9 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
10 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
11 Oxidative cyclization reagents reveal tryptophan cation- interactions. Nature. 2024 Mar;627(8004):680-687. doi: 10.1038/s41586-024-07140-6. Epub 2024 Mar 6.
Mass spectrometry data entry: PXD001377 , PXD005252
12 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
13 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
14 Differently Tagged Probes for Protein Profiling of Mitochondria. Chembiochem. 2019 May 2;20(9):1155-1160. doi: 10.1002/cbic.201800735. Epub 2019 Mar 26.
15 Ligand and Target Discovery by Fragment-Based Screening in Human Cells. Cell. 2017 Jan 26;168(3):527-541.e29. doi: 10.1016/j.cell.2016.12.029. Epub 2017 Jan 19.
16 Mapping Protein Targets of Bioactive Small Molecules Using Lipid-Based Chemical Proteomics. ACS Chem Biol. 2017 Oct 20;12(10):2671-2681. doi: 10.1021/acschembio.7b00581. Epub 2017 Sep 20.
Mass spectrometry data entry: PXD007570
17 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
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