Details of the Target

General Information of Target

Target ID LDTP01135
Target Name GATOR1 complex protein DEPDC5 (DEPDC5)
Gene Name DEPDC5
Gene ID 9681
Synonyms
KIAA0645; GATOR1 complex protein DEPDC5; DEP domain-containing protein 5
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MRTTKVYKLVIHKKGFGGSDDELVVNPKVFPHIKLGDIVEIAHPNDEYSPLLLQVKSLKE
DLQKETISVDQTVTQVFRLRPYQDVYVNVVDPKDVTLDLVELTFKDQYIGRGDMWRLKKS
LVSTCAYITQKVEFAGIRAQAGELWVKNEKVMCGYISEDTRVVFRSTSAMVYIFIQMSCE
MWDFDIYGDLYFEKAVNGFLADLFTKWKEKNCSHEVTVVLFSRTFYDAKSVDEFPEINRA
SIRQDHKGRFYEDFYKVVVQNERREEWTSLLVTIKKLFIQYPVLVRLEQAEGFPQGDNST
SAQGNYLEAINLSFNVFDKHYINRNFDRTGQMSVVITPGVGVFEVDRLLMILTKQRMIDN
GIGVDLVCMGEQPLHAVPLFKLHNRSAPRDSRLGDDYNIPHWINHSFYTSKSQLFCNSFT
PRIKLAGKKPASEKAKNGRDTSLGSPKESENALPIQVDYDAYDAQVFRLPGPSRAQCLTT
CRSVRERESHSRKSASSCDVSSSPSLPSRTLPTEEVRSQASDDSSLGKSANILMIPHPHL
HQYEVSSSLGYTSTRDVLENMMEPPQRDSSAPGRFHVGSAESMLHVRPGGYTPQRALINP
FAPSRMPMKLTSNRRRWMHTFPVGPSGEAIQIHHQTRQNMAELQGSGQRDPTHSSAELLE
LAYHEAAGRHSNSRQPGDGMSFLNFSGTEELSVGLLSNSGAGMNPRTQNKDSLEDSVSTS
PDPILTLSAPPVVPGFCCTVGVDWKSLTTPACLPLTTDYFPDRQGLQNDYTEGCYDLLPE
ADIDRRDEDGVQMTAQQVFEEFICQRLMQGYQIIVQPKTQKPNPAVPPPLSSSPLYSRGL
VSRNRPEEEDQYWLSMGRTFHKVTLKDKMITVTRYLPKYPYESAQIHYTYSLCPSHSDSE
FVSCWVEFSHERLEEYKWNYLDQYICSAGSEDFSLIESLKFWRTRFLLLPACVTATKRIT
EGEAHCDIYGDRPRADEDEWQLLDGFVRFVEGLNRIRRRHRSDRMMRKGTAMKGLQMTGP
ISTHSLESTAPPVGKKGTSALSALLEMEASQKCLGEQQAAVHGGKSSAQSAESSSVAMTP
TYMDSPRKDGAFFMEFVRSPRTASSAFYPQVSVDQTATPMLDGTSLGICTGQSMDRGNSQ
TFGNSQNIGEQGYSSTNSSDSSSQQLVASSLTSSSTLTEILEAMKHPSTGVQLLSEQKGL
SPYCFISAEVVHWLVNHVEGIQTQAMAIDIMQKMLEEQLITHASGEAWRTFIYGFYFYKI
VTDKEPDRVAMQQPATTWHTAGVDDFASFQRKWFEVAFVAEELVHSEIPAFLLPWLPSRP
ASYASRHSSFSRSFGGRSQAAALLAATVPEQRTVTLDVDVNNRTDRLEWCSCYYHGNFSL
NAAFEIKLHWMAVTAAVLFEMVQGWHRKATSCGFLLVPVLEGPFALPSYLYGDPLRAQLF
IPLNISCLLKEGSEHLFDSFEPETYWDRMHLFQEAIAHRFGFVQDKYSASAFNFPAENKP
QYIHVTGTVFLQLPYSKRKFSGQQRRRRNSTSSTNQNMFCEERVGYNWAYNTMLTKTWRS
SATGDEKFADRLLKDFTDFCINRDNRLVTFWTSCLEKMHASAP
Target Bioclass
Other
Family
IML1 family
Subcellular location
Cytoplasm, cytosol
Function
As a component of the GATOR1 complex functions as an inhibitor of the amino acid-sensing branch of the mTORC1 pathway . In response to amino acid depletion, the GATOR1 complex has GTPase activating protein (GAP) activity and strongly increases GTP hydrolysis by RagA/RRAGA (or RagB/RRAGB) within heterodimeric Rag complexes, thereby turning them into their inactive GDP-bound form, releasing mTORC1 from lysosomal surface and inhibiting mTORC1 signaling. In the presence of abundant amino acids, the GATOR1 complex is negatively regulated by GATOR2, the other GATOR subcomplex, in this amino acid-sensing branch of the TORC1 pathway. Within the GATOR1 complex, DEPDC5 mediates direct interaction with the nucleotide-binding pocket of small GTPases Rag (RagA/RRAGA, RagB/RRAGB, RagC/RRAGC and/or RagD/RRAGD) and coordinates their nucleotide loading states by promoting RagA/RRAGA or RagB/RRAGB into their GDP-binding state and RagC/RRAGC or RagD/RRAGD into their GTP-binding state. However, it does not execute the GAP activity, which is mediated by NPRL2.
Uniprot ID
O75140
Ensemble ID
ENST00000382111.6
HGNC ID
HGNC:18423

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 4 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C416(1.00)  LDD0531  [1]
IA-alkyne
 Probe Info  		N.A.  LDD0032  [2]
NAIA_5
 Probe Info  		N.A.  LDD2225  [3]
IPM
 Probe Info  		C498(0.00); C1540(0.00); C416(0.00); C1053(0.00)  LDD2156  [4]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0214  AC1 HCT 116 C416(1.00)  LDD0531  [1]
 LDCM0215  AC10 HCT 116 C477(1.21); C481(1.21)  LDD0532  [1]
 LDCM0216  AC100 HCT 116 C416(1.44)  LDD0533  [1]
 LDCM0217  AC101 HCT 116 C416(1.34)  LDD0534  [1]
 LDCM0218  AC102 HCT 116 C416(1.65)  LDD0535  [1]
 LDCM0219  AC103 HCT 116 C416(2.10)  LDD0536  [1]
 LDCM0220  AC104 HCT 116 C416(1.86)  LDD0537  [1]
 LDCM0221  AC105 HCT 116 C416(2.77)  LDD0538  [1]
 LDCM0222  AC106 HCT 116 C416(1.71)  LDD0539  [1]
 LDCM0223  AC107 HCT 116 C416(2.31)  LDD0540  [1]
 LDCM0224  AC108 HCT 116 C416(1.18)  LDD0541  [1]
 LDCM0225  AC109 HCT 116 C416(1.66)  LDD0542  [1]
 LDCM0226  AC11 HCT 116 C477(1.26); C481(1.26)  LDD0543  [1]
 LDCM0227  AC110 HCT 116 C416(1.31)  LDD0544  [1]
 LDCM0228  AC111 HCT 116 C416(1.35)  LDD0545  [1]
 LDCM0229  AC112 HCT 116 C416(1.54)  LDD0546  [1]
 LDCM0230  AC113 HCT 116 C477(0.80); C481(0.80)  LDD0547  [1]
 LDCM0231  AC114 HCT 116 C477(1.44); C481(1.44)  LDD0548  [1]
 LDCM0232  AC115 HCT 116 C477(1.73); C481(1.73)  LDD0549  [1]
 LDCM0233  AC116 HCT 116 C477(4.45); C481(4.45)  LDD0550  [1]
 LDCM0234  AC117 HCT 116 C477(1.19); C481(1.19)  LDD0551  [1]
 LDCM0235  AC118 HCT 116 C477(1.17); C481(1.17)  LDD0552  [1]
 LDCM0236  AC119 HCT 116 C477(1.32); C481(1.32)  LDD0553  [1]
 LDCM0237  AC12 HCT 116 C477(1.09); C481(1.09)  LDD0554  [1]
 LDCM0238  AC120 HCT 116 C477(1.52); C481(1.52)  LDD0555  [1]
 LDCM0239  AC121 HCT 116 C477(0.85); C481(0.85)  LDD0556  [1]
 LDCM0240  AC122 HCT 116 C477(1.05); C481(1.05)  LDD0557  [1]
 LDCM0241  AC123 HCT 116 C477(0.83); C481(0.83)  LDD0558  [1]
 LDCM0242  AC124 HCT 116 C477(1.51); C481(1.51)  LDD0559  [1]
 LDCM0243  AC125 HCT 116 C477(1.38); C481(1.38)  LDD0560  [1]
 LDCM0244  AC126 HCT 116 C477(2.29); C481(2.29)  LDD0561  [1]
 LDCM0245  AC127 HCT 116 C477(1.29); C481(1.29)  LDD0562  [1]
 LDCM0259  AC14 HCT 116 C477(0.97); C481(0.97)  LDD0576  [1]
 LDCM0263  AC143 HCT 116 C416(1.10)  LDD0580  [1]
 LDCM0264  AC144 HCT 116 C416(1.31)  LDD0581  [1]
 LDCM0265  AC145 HCT 116 C416(1.19)  LDD0582  [1]
 LDCM0266  AC146 HCT 116 C416(1.56)  LDD0583  [1]
 LDCM0267  AC147 HCT 116 C416(1.31)  LDD0584  [1]
 LDCM0268  AC148 HCT 116 C416(1.65)  LDD0585  [1]
 LDCM0269  AC149 HCT 116 C416(1.41)  LDD0586  [1]
 LDCM0270  AC15 HCT 116 C477(1.04); C481(1.04)  LDD0587  [1]
 LDCM0271  AC150 HCT 116 C416(1.36)  LDD0588  [1]
 LDCM0272  AC151 HCT 116 C416(1.29)  LDD0589  [1]
 LDCM0273  AC152 HCT 116 C416(1.33)  LDD0590  [1]
 LDCM0274  AC153 HCT 116 C416(1.66)  LDD0591  [1]
 LDCM0621  AC154 HCT 116 C416(1.32)  LDD2158  [1]
 LDCM0622  AC155 HCT 116 C416(1.58)  LDD2159  [1]
 LDCM0623  AC156 HCT 116 C416(1.46)  LDD2160  [1]
 LDCM0624  AC157 HCT 116 C416(1.31)  LDD2161  [1]
 LDCM0276  AC17 HCT 116 C416(0.88)  LDD0593  [1]
 LDCM0277  AC18 HCT 116 C416(0.95)  LDD0594  [1]
 LDCM0278  AC19 HCT 116 C416(0.95)  LDD0595  [1]
 LDCM0279  AC2 HCT 116 C416(1.03)  LDD0596  [1]
 LDCM0280  AC20 HCT 116 C416(0.87)  LDD0597  [1]
 LDCM0281  AC21 HCT 116 C416(0.90)  LDD0598  [1]
 LDCM0282  AC22 HCT 116 C416(1.03)  LDD0599  [1]
 LDCM0283  AC23 HCT 116 C416(0.93)  LDD0600  [1]
 LDCM0284  AC24 HCT 116 C416(0.83)  LDD0601  [1]
 LDCM0285  AC25 HEK-293T C1053(1.04); C952(1.12); C481(0.96); C477(1.05)  LDD1524  [5]
 LDCM0286  AC26 HEK-293T C1053(0.93); C481(1.02); C477(0.97)  LDD1525  [5]
 LDCM0287  AC27 HEK-293T C1053(1.04); C477(1.14)  LDD1526  [5]
 LDCM0288  AC28 HEK-293T C1053(1.00); C952(1.04); C477(1.07)  LDD1527  [5]
 LDCM0289  AC29 HEK-293T C1053(0.98); C952(1.10); C477(0.81)  LDD1528  [5]
 LDCM0290  AC3 HCT 116 C416(1.09)  LDD0607  [1]
 LDCM0291  AC30 HEK-293T C952(0.99)  LDD1530  [5]
 LDCM0292  AC31 HEK-293T C481(1.11)  LDD1531  [5]
 LDCM0293  AC32 HEK-293T C952(1.00)  LDD1532  [5]
 LDCM0294  AC33 HEK-293T C1053(1.04); C952(1.09); C481(1.12); C477(1.26)  LDD1533  [5]
 LDCM0295  AC34 HEK-293T C1053(1.02); C481(1.00); C477(1.27)  LDD1534  [5]
 LDCM0296  AC35 HEK-293T C477(1.17); C481(1.17)  LDD0894  [1]
 LDCM0297  AC36 HEK-293T C477(1.12); C481(1.12)  LDD0895  [1]
 LDCM0298  AC37 HEK-293T C477(1.17); C481(1.17)  LDD0896  [1]
 LDCM0299  AC38 HEK-293T C477(1.36); C481(1.36)  LDD0897  [1]
 LDCM0300  AC39 HEK-293T C477(1.40); C481(1.40)  LDD0898  [1]
 LDCM0301  AC4 HCT 116 C416(1.08)  LDD0618  [1]
 LDCM0302  AC40 HEK-293T C477(1.29); C481(1.29)  LDD0900  [1]
 LDCM0303  AC41 HEK-293T C477(1.02); C481(1.02)  LDD0901  [1]
 LDCM0304  AC42 HEK-293T C477(1.04); C481(1.04)  LDD0902  [1]
 LDCM0305  AC43 HEK-293T C477(1.15); C481(1.15)  LDD0903  [1]
 LDCM0306  AC44 HEK-293T C477(1.08); C481(1.08)  LDD0904  [1]
 LDCM0307  AC45 HEK-293T C477(1.31); C481(1.31)  LDD0905  [1]
 LDCM0308  AC46 HEK-293T C477(0.86); C481(0.86)  LDD0906  [1]
 LDCM0309  AC47 HEK-293T C477(0.94); C481(0.94)  LDD0907  [1]
 LDCM0310  AC48 HEK-293T C477(0.83); C481(0.83)  LDD0908  [1]
 LDCM0311  AC49 HEK-293T C477(0.98); C481(0.98)  LDD0909  [1]
 LDCM0312  AC5 HCT 116 C416(1.00)  LDD0629  [1]
 LDCM0313  AC50 HEK-293T C477(1.06); C481(1.06)  LDD0911  [1]
 LDCM0314  AC51 HEK-293T C477(1.11); C481(1.11)  LDD0912  [1]
 LDCM0315  AC52 HEK-293T C477(1.13); C481(1.13)  LDD0913  [1]
 LDCM0316  AC53 HEK-293T C477(1.60); C481(1.60)  LDD0914  [1]
 LDCM0317  AC54 HEK-293T C477(1.17); C481(1.17)  LDD0915  [1]
 LDCM0318  AC55 HEK-293T C477(1.40); C481(1.40)  LDD0916  [1]
 LDCM0319  AC56 HEK-293T C477(0.91); C481(0.91)  LDD0917  [1]
 LDCM0320  AC57 HCT 116 C416(0.99)  LDD0637  [1]
 LDCM0321  AC58 HCT 116 C416(1.00)  LDD0638  [1]
 LDCM0322  AC59 HCT 116 C416(1.06)  LDD0639  [1]
 LDCM0323  AC6 HCT 116 C477(1.10); C481(1.10)  LDD0640  [1]
 LDCM0324  AC60 HCT 116 C416(1.08)  LDD0641  [1]
 LDCM0325  AC61 HCT 116 C416(0.91)  LDD0642  [1]
 LDCM0326  AC62 HCT 116 C416(1.27)  LDD0643  [1]
 LDCM0327  AC63 HCT 116 C416(1.08)  LDD0644  [1]
 LDCM0328  AC64 HCT 116 C416(1.24)  LDD0645  [1]
 LDCM0329  AC65 HCT 116 C416(0.97)  LDD0646  [1]
 LDCM0330  AC66 HCT 116 C416(1.03)  LDD0647  [1]
 LDCM0331  AC67 HCT 116 C416(1.08)  LDD0648  [1]
 LDCM0332  AC68 HCT 116 C416(1.04)  LDD0649  [1]
 LDCM0333  AC69 HCT 116 C416(1.05)  LDD0650  [1]
 LDCM0334  AC7 HCT 116 C477(1.07); C481(1.07)  LDD0651  [1]
 LDCM0335  AC70 HCT 116 C416(0.93)  LDD0652  [1]
 LDCM0336  AC71 HCT 116 C416(1.08)  LDD0653  [1]
 LDCM0337  AC72 HCT 116 C416(1.12)  LDD0654  [1]
 LDCM0338  AC73 HCT 116 C416(0.99)  LDD0655  [1]
 LDCM0339  AC74 HCT 116 C416(1.14)  LDD0656  [1]
 LDCM0340  AC75 HCT 116 C416(1.10)  LDD0657  [1]
 LDCM0341  AC76 HCT 116 C416(0.98)  LDD0658  [1]
 LDCM0342  AC77 HCT 116 C416(0.96)  LDD0659  [1]
 LDCM0343  AC78 HCT 116 C416(1.06)  LDD0660  [1]
 LDCM0344  AC79 HCT 116 C416(1.16)  LDD0661  [1]
 LDCM0345  AC8 HCT 116 C477(1.15); C481(1.15)  LDD0662  [1]
 LDCM0346  AC80 HCT 116 C416(1.19)  LDD0663  [1]
 LDCM0347  AC81 HCT 116 C416(1.03)  LDD0664  [1]
 LDCM0348  AC82 HCT 116 C416(0.96)  LDD0665  [1]
 LDCM0365  AC98 HCT 116 C416(1.60)  LDD0682  [1]
 LDCM0366  AC99 HCT 116 C416(1.44)  LDD0683  [1]
 LDCM0248  AKOS034007472 HCT 116 C477(1.07); C481(1.07)  LDD0565  [1]
 LDCM0356  AKOS034007680 HCT 116 C477(1.25); C481(1.25)  LDD0673  [1]
 LDCM0275  AKOS034007705 HCT 116 C477(1.16); C481(1.16)  LDD0592  [1]
 LDCM0367  CL1 HEK-293T C1053(0.92); C952(0.92)  LDD1571  [5]
 LDCM0368  CL10 HEK-293T C952(0.71)  LDD1572  [5]
 LDCM0369  CL100 HCT 116 C416(1.13)  LDD0686  [1]
 LDCM0370  CL101 HCT 116 C477(0.98); C481(0.98)  LDD0687  [1]
 LDCM0371  CL102 HCT 116 C477(1.02); C481(1.02)  LDD0688  [1]
 LDCM0372  CL103 HCT 116 C477(1.13); C481(1.13)  LDD0689  [1]
 LDCM0373  CL104 HCT 116 C477(1.19); C481(1.19)  LDD0690  [1]
 LDCM0374  CL105 HCT 116 C416(0.95)  LDD0691  [1]
 LDCM0375  CL106 HCT 116 C416(1.09)  LDD0692  [1]
 LDCM0376  CL107 HCT 116 C416(0.99)  LDD0693  [1]
 LDCM0377  CL108 HCT 116 C416(0.91)  LDD0694  [1]
 LDCM0378  CL109 HCT 116 C416(1.05)  LDD0695  [1]
 LDCM0379  CL11 HEK-293T C481(1.19)  LDD1583  [5]
 LDCM0380  CL110 HCT 116 C416(0.87)  LDD0697  [1]
 LDCM0381  CL111 HCT 116 C416(1.06)  LDD0698  [1]
 LDCM0382  CL112 HEK-293T C1053(1.15); C481(1.06)  LDD1586  [5]
 LDCM0383  CL113 HEK-293T C1053(0.94); C952(1.05)  LDD1587  [5]
 LDCM0384  CL114 HEK-293T C1053(0.92); C952(0.96); C481(1.01); C477(0.99)  LDD1588  [5]
 LDCM0385  CL115 HEK-293T C1053(0.89); C952(1.11)  LDD1589  [5]
 LDCM0386  CL116 HEK-293T C1053(0.99); C481(0.93)  LDD1590  [5]
 LDCM0387  CL117 HEK-293T C477(1.35); C481(1.35)  LDD0985  [1]
 LDCM0388  CL118 HEK-293T C477(0.87); C481(0.87)  LDD0986  [1]
 LDCM0389  CL119 HEK-293T C477(0.90); C481(0.90)  LDD0987  [1]
 LDCM0390  CL12 HEK-293T C952(0.96)  LDD1594  [5]
 LDCM0391  CL120 HEK-293T C477(1.15); C481(1.15)  LDD0989  [1]
 LDCM0392  CL121 HEK-293T C477(1.28); C481(1.28)  LDD0990  [1]
 LDCM0393  CL122 HEK-293T C477(1.11); C481(1.11)  LDD0991  [1]
 LDCM0394  CL123 HEK-293T C477(2.71); C481(2.71)  LDD0992  [1]
 LDCM0395  CL124 HEK-293T C477(1.20); C481(1.20)  LDD0993  [1]
 LDCM0396  CL125 HCT 116 C416(1.17)  LDD0713  [1]
 LDCM0397  CL126 HCT 116 C416(1.01)  LDD0714  [1]
 LDCM0398  CL127 HCT 116 C416(1.07)  LDD0715  [1]
 LDCM0399  CL128 HCT 116 C416(0.98)  LDD0716  [1]
 LDCM0400  CL13 HEK-293T C1053(1.11); C952(0.95)  LDD1604  [5]
 LDCM0401  CL14 HEK-293T C1053(0.88); C952(0.95); C481(1.26); C477(0.86)  LDD1605  [5]
 LDCM0402  CL15 HEK-293T C1053(0.85); C952(1.08)  LDD1606  [5]
 LDCM0403  CL16 HCT 116 C416(1.39)  LDD0720  [1]
 LDCM0404  CL17 HCT 116 C416(1.12)  LDD0721  [1]
 LDCM0405  CL18 HCT 116 C416(1.13)  LDD0722  [1]
 LDCM0406  CL19 HCT 116 C416(1.10)  LDD0723  [1]
 LDCM0407  CL2 HEK-293T C1053(0.96); C952(0.97); C481(1.03); C477(0.98)  LDD1611  [5]
 LDCM0408  CL20 HCT 116 C416(1.09)  LDD0725  [1]
 LDCM0409  CL21 HCT 116 C416(1.11)  LDD0726  [1]
 LDCM0410  CL22 HCT 116 C416(1.16)  LDD0727  [1]
 LDCM0411  CL23 HCT 116 C416(1.15)  LDD0728  [1]
 LDCM0412  CL24 HCT 116 C416(1.06)  LDD0729  [1]
 LDCM0413  CL25 HCT 116 C416(1.22)  LDD0730  [1]
 LDCM0414  CL26 HCT 116 C416(1.21)  LDD0731  [1]
 LDCM0415  CL27 HCT 116 C416(1.13)  LDD0732  [1]
 LDCM0416  CL28 HCT 116 C416(1.05)  LDD0733  [1]
 LDCM0417  CL29 HCT 116 C416(1.04)  LDD0734  [1]
 LDCM0418  CL3 HEK-293T C1053(0.85); C952(1.12)  LDD1622  [5]
 LDCM0419  CL30 HCT 116 C416(1.04)  LDD0736  [1]
 LDCM0420  CL31 HCT 116 C416(0.96)  LDD0737  [1]
 LDCM0421  CL32 HEK-293T C477(0.91); C481(0.91)  LDD1019  [1]
 LDCM0422  CL33 HEK-293T C477(1.57); C481(1.57)  LDD1020  [1]
 LDCM0423  CL34 HEK-293T C477(1.09); C481(1.09)  LDD1021  [1]
 LDCM0424  CL35 HEK-293T C477(1.28); C481(1.28)  LDD1022  [1]
 LDCM0425  CL36 HEK-293T C477(1.45); C481(1.45)  LDD1023  [1]
 LDCM0426  CL37 HEK-293T C477(1.42); C481(1.42)  LDD1024  [1]
 LDCM0428  CL39 HEK-293T C477(1.30); C481(1.30)  LDD1026  [1]
 LDCM0429  CL4 HEK-293T C1053(1.08); C481(1.10)  LDD1633  [5]
 LDCM0430  CL40 HEK-293T C477(0.99); C481(0.99)  LDD1028  [1]
 LDCM0431  CL41 HEK-293T C477(1.10); C481(1.10)  LDD1029  [1]
 LDCM0432  CL42 HEK-293T C477(1.26); C481(1.26)  LDD1030  [1]
 LDCM0433  CL43 HEK-293T C477(1.60); C481(1.60)  LDD1031  [1]
 LDCM0434  CL44 HEK-293T C477(1.13); C481(1.13)  LDD1032  [1]
 LDCM0435  CL45 HEK-293T C477(1.20); C481(1.20)  LDD1033  [1]
 LDCM0436  CL46 HEK-293T C952(0.90)  LDD1640  [5]
 LDCM0437  CL47 HEK-293T C481(0.99)  LDD1641  [5]
 LDCM0438  CL48 HEK-293T C952(1.01)  LDD1642  [5]
 LDCM0439  CL49 HEK-293T C1053(0.98); C952(0.94)  LDD1643  [5]
 LDCM0440  CL5 HEK-293T C1053(1.13); C952(1.04); C481(0.97); C477(1.17)  LDD1644  [5]
 LDCM0441  CL50 HEK-293T C1053(1.01); C952(1.04); C481(1.03); C477(0.95)  LDD1645  [5]
 LDCM0443  CL52 HEK-293T C1053(1.04); C481(0.96)  LDD1646  [5]
 LDCM0444  CL53 HEK-293T C1053(0.97); C952(0.96); C481(0.76); C477(1.00)  LDD1647  [5]
 LDCM0445  CL54 HEK-293T C1053(0.97); C481(0.94); C477(0.98)  LDD1648  [5]
 LDCM0446  CL55 HEK-293T C1053(1.01); C477(1.17)  LDD1649  [5]
 LDCM0447  CL56 HEK-293T C1053(1.00); C952(0.93); C477(1.06)  LDD1650  [5]
 LDCM0448  CL57 HEK-293T C1053(1.02); C952(0.92); C477(1.00)  LDD1651  [5]
 LDCM0449  CL58 HEK-293T C952(0.95)  LDD1652  [5]
 LDCM0450  CL59 HEK-293T C481(1.09)  LDD1653  [5]
 LDCM0451  CL6 HEK-293T C1053(0.99); C481(1.00); C477(1.05)  LDD1654  [5]
 LDCM0452  CL60 HEK-293T C952(0.92)  LDD1655  [5]
 LDCM0453  CL61 HCT 116 C477(1.03); C481(1.03)  LDD0770  [1]
 LDCM0454  CL62 HCT 116 C477(1.08); C481(1.08)  LDD0771  [1]
 LDCM0455  CL63 HCT 116 C477(1.11); C481(1.11)  LDD0772  [1]
 LDCM0456  CL64 HCT 116 C477(0.91); C481(0.91)  LDD0773  [1]
 LDCM0457  CL65 HCT 116 C477(1.20); C481(1.20)  LDD0774  [1]
 LDCM0458  CL66 HCT 116 C477(0.95); C481(0.95)  LDD0775  [1]
 LDCM0459  CL67 HCT 116 C477(1.00); C481(1.00)  LDD0776  [1]
 LDCM0460  CL68 HCT 116 C477(0.76); C481(0.76)  LDD0777  [1]
 LDCM0461  CL69 HCT 116 C477(1.23); C481(1.23)  LDD0778  [1]
 LDCM0462  CL7 HEK-293T C1053(1.02); C477(1.01)  LDD1665  [5]
 LDCM0463  CL70 HCT 116 C477(0.95); C481(0.95)  LDD0780  [1]
 LDCM0464  CL71 HCT 116 C477(1.01); C481(1.01)  LDD0781  [1]
 LDCM0465  CL72 HCT 116 C477(0.98); C481(0.98)  LDD0782  [1]
 LDCM0466  CL73 HCT 116 C477(0.97); C481(0.97)  LDD0783  [1]
 LDCM0467  CL74 HCT 116 C477(1.21); C481(1.21)  LDD0784  [1]
 LDCM0469  CL76 HCT 116 C477(0.81); C481(0.81); C416(0.84)  LDD0786  [1]
 LDCM0470  CL77 HCT 116 C477(0.78); C481(0.78); C416(0.85)  LDD0787  [1]
 LDCM0471  CL78 HCT 116 C477(1.30); C481(1.30); C416(1.19)  LDD0788  [1]
 LDCM0472  CL79 HCT 116 C477(0.73); C481(0.73); C416(0.85)  LDD0789  [1]
 LDCM0473  CL8 HEK-293T C1053(0.96); C952(0.99); C477(1.01)  LDD1676  [5]
 LDCM0474  CL80 HCT 116 C477(0.82); C481(0.82); C416(0.86)  LDD0791  [1]
 LDCM0475  CL81 HCT 116 C477(0.71); C481(0.71); C416(0.84)  LDD0792  [1]
 LDCM0476  CL82 HCT 116 C477(0.64); C481(0.64); C416(0.78)  LDD0793  [1]
 LDCM0477  CL83 HCT 116 C477(0.73); C481(0.73); C416(0.91)  LDD0794  [1]
 LDCM0478  CL84 HCT 116 C477(0.93); C481(0.93); C416(0.88)  LDD0795  [1]
 LDCM0479  CL85 HCT 116 C477(0.88); C481(0.88); C416(0.88)  LDD0796  [1]
 LDCM0480  CL86 HCT 116 C477(0.97); C481(0.97); C416(0.85)  LDD0797  [1]
 LDCM0481  CL87 HCT 116 C477(0.82); C481(0.82); C416(0.80)  LDD0798  [1]
 LDCM0482  CL88 HCT 116 C477(0.68); C481(0.68); C416(0.86)  LDD0799  [1]
 LDCM0483  CL89 HCT 116 C477(0.82); C481(0.82); C416(0.97)  LDD0800  [1]
 LDCM0484  CL9 HEK-293T C1053(0.97); C952(0.97); C477(0.93)  LDD1687  [5]
 LDCM0485  CL90 HCT 116 C477(1.02); C481(1.02); C416(1.03)  LDD0802  [1]
 LDCM0486  CL91 HCT 116 C416(1.08)  LDD0803  [1]
 LDCM0487  CL92 HCT 116 C416(1.32)  LDD0804  [1]
 LDCM0488  CL93 HCT 116 C416(0.97)  LDD0805  [1]
 LDCM0489  CL94 HCT 116 C416(1.10)  LDD0806  [1]
 LDCM0490  CL95 HCT 116 C416(0.98)  LDD0807  [1]
 LDCM0491  CL96 HCT 116 C416(0.99)  LDD0808  [1]
 LDCM0492  CL97 HCT 116 C416(1.19)  LDD0809  [1]
 LDCM0493  CL98 HCT 116 C416(1.30)  LDD0810  [1]
 LDCM0494  CL99 HCT 116 C416(1.03)  LDD0811  [1]
 LDCM0495  E2913 HEK-293T C1053(0.93); C952(0.96)  LDD1698  [5]
 LDCM0468  Fragment33 HCT 116 C477(0.87); C481(0.87)  LDD0785  [1]
 LDCM0427  Fragment51 HEK-293T C477(1.14); C481(1.14)  LDD1025  [1]
 LDCM0022  KB02 T cell-activated C477, C481(16.74)  LDD1704  [6]
 LDCM0023  KB03 697 C952(1.99)  LDD2662  [7]
 LDCM0024  KB05 MV4-11 C952(1.66)  LDD3338  [7]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Ubiquitin carboxyl-terminal hydrolase 2 (USP2) Peptidase C19 family O75604
Myotubularin-related protein 9 (MTMR9) Protein-tyrosine phosphatase family Q96QG7
Other
Click To Hide/Show 5 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Mitotic spindle assembly checkpoint protein MAD2A (MAD2L1) MAD2 family Q13257
Synaptotagmin-17 (SYT17) Synaptotagmin family Q9BSW7
T-complex protein 1 subunit gamma (CCT3) TCP-1 chaperonin family P49368
Developmental pluripotency-associated protein 3 (DPPA3) . Q6W0C5
Mitotic spindle assembly checkpoint protein MAD2B (MAD2L2) . Q9UI95

References

1 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
2 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
3 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
4 Benchmarking Cleavable Biotin Tags for Peptide-Centric Chemoproteomics. J Proteome Res. 2022 May 6;21(5):1349-1358. doi: 10.1021/acs.jproteome.2c00174. Epub 2022 Apr 25.
Mass spectrometry data entry: PXD031019
5 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
6 An Activity-Guided Map of Electrophile-Cysteine Interactions in Primary Human T Cells. Cell. 2020 Aug 20;182(4):1009-1026.e29. doi: 10.1016/j.cell.2020.07.001. Epub 2020 Jul 29.
7 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840