Details of the Target

General Information of Target

Target ID LDTP00363
Target Name F-BAR domain only protein 1 (FCHO1)
Gene Name FCHO1
Gene ID 23149
Synonyms
KIAA0290; F-BAR domain only protein 1
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MSYFGEHFWGEKNHGFEVLYHSVKQGPISTKELADFIRERATIEETYSKAMAKLSKLASN
GTPMGTFAPLWEVFRVSSDKLALCHLELTRKLQDLIKDVLRYGEEQLKTHKKCKEEVVST
LDAVQVLSGVSQLLPKSRENYLNRCMDQERLRRESTSQKEMDKAETKTKKAAESLRRSVE
KYNSARADFEQKMLDSALRFQAMEETHLRHMKALLGSYAHSVEDTHVQIGQVHEEFKQNI
ENVSVEMLLRKFAESKGTGREKPGPLDFEAYSAAALQEAMKRLRGAKAFRLPGLSRRERE
PEPPAAVDFLEPDSGTCPEVDEEGFTVRPDVTQNSTAEPSRFSSSDSDFDDEEPRKFYVH
IKPAPARAPACSPEAAAAQLRATAGSLILPPGPGGTMKRHSSRDAAGKPQRPRSAPRTSS
CAERLQSEEQVSKNLFGPPLESAFDHEDFTGSSSLGFTSSPSPFSSSSPENVEDSGLDSP
SHAAPGPSPDSWVPRPGTPQSPPSCRAPPPEARGIRAPPLPDSPQPLASSPGPWGLEALA
GGDLMPAPADPTAREGLAAPPRRLRSRKVSCPLTRSNGDLSRSLSPSPLGSSAASTALER
PSFLSQTGHGVSRGPSPVVLGSQDALPIATAFTEYVHAYFRGHSPSCLARVTGELTMTFP
AGIVRVFSGTPPPPVLSFRLVHTTAIEHFQPNADLLFSDPSQSDPETKDFWLNMAALTEA
LQRQAEQNPTASYYNVVLLRYQFSRPGPQSVPLQLSAHWQCGATLTQVSVEYGYRPGATA
VPTPLTNVQILLPVGEPVTNVRLQPAATWNLEEKRLTWRLPDVSEAGGSGRLSASWEPLS
GPSTPSPVAAQFTSEGTTLSGVDLELVGSGYRMSLVKRRFATGMYLVSC
Target Bioclass
Other
Family
FCHO family
Subcellular location
Membrane, clathrin-coated pit
Function
Functions in an early step of clathrin-mediated endocytosis. Has both a membrane binding/bending activity and the ability to recruit proteins essential to the formation of functional clathrin-coated pits. May regulate Bmp signaling by regulating clathrin-mediated endocytosis of Bmp receptors. Involved in the regulation of T-cell poliferation and activation. Affects TCR clustering upon receptor triggering and modulates its internalisation, playing a role in TCR-dependent T-cell activation.
Uniprot ID
O14526
Ensemble ID
ENST00000595033.5
HGNC ID
HGNC:29002

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
IM95 Deletion: p.W9GfsTer15 .
MDAMB231 SNV: p.E17K .
NCIH716 SNV: p.A275G .
SSP25 SNV: p.A517E .
T24 SNV: p.D478N .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 4 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C371(5.13); C571(9.05)  LDD0209  [1]
4-Iodoacetamidophenylacetylene
 Probe Info  		C421(0.00); C647(0.00)  LDD0038  [2]
IA-alkyne
 Probe Info  		N.A.  LDD0166  [3]
Lodoacetamide azide
 Probe Info  		N.A.  LDD0037  [2]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0216  AC100 HCT 116 C889(1.22)  LDD0533  [4]
 LDCM0217  AC101 HCT 116 C889(1.74)  LDD0534  [4]
 LDCM0218  AC102 HCT 116 C889(1.57)  LDD0535  [4]
 LDCM0219  AC103 HCT 116 C889(2.02)  LDD0536  [4]
 LDCM0220  AC104 HCT 116 C889(1.70)  LDD0537  [4]
 LDCM0221  AC105 HCT 116 C889(2.18)  LDD0538  [4]
 LDCM0222  AC106 HCT 116 C889(2.42)  LDD0539  [4]
 LDCM0223  AC107 HCT 116 C889(1.87)  LDD0540  [4]
 LDCM0224  AC108 HCT 116 C889(1.50)  LDD0541  [4]
 LDCM0225  AC109 HCT 116 C889(1.44)  LDD0542  [4]
 LDCM0227  AC110 HCT 116 C889(1.61)  LDD0544  [4]
 LDCM0228  AC111 HCT 116 C889(1.46)  LDD0545  [4]
 LDCM0229  AC112 HCT 116 C889(1.66)  LDD0546  [4]
 LDCM0263  AC143 HCT 116 C889(0.90)  LDD0580  [4]
 LDCM0264  AC144 HCT 116 C889(1.58)  LDD0581  [4]
 LDCM0265  AC145 HCT 116 C889(1.18)  LDD0582  [4]
 LDCM0266  AC146 HCT 116 C889(1.64)  LDD0583  [4]
 LDCM0267  AC147 HCT 116 C889(1.45)  LDD0584  [4]
 LDCM0268  AC148 HCT 116 C889(2.52)  LDD0585  [4]
 LDCM0269  AC149 HCT 116 C889(1.70)  LDD0586  [4]
 LDCM0271  AC150 HCT 116 C889(1.12)  LDD0588  [4]
 LDCM0272  AC151 HCT 116 C889(1.24)  LDD0589  [4]
 LDCM0273  AC152 HCT 116 C889(1.70)  LDD0590  [4]
 LDCM0274  AC153 HCT 116 C889(2.82)  LDD0591  [4]
 LDCM0621  AC154 HCT 116 C889(1.64)  LDD2158  [4]
 LDCM0622  AC155 HCT 116 C889(1.34)  LDD2159  [4]
 LDCM0623  AC156 HCT 116 C889(1.10)  LDD2160  [4]
 LDCM0624  AC157 HCT 116 C889(0.95)  LDD2161  [4]
 LDCM0276  AC17 HCT 116 C889(0.94)  LDD0593  [4]
 LDCM0277  AC18 HCT 116 C889(1.90)  LDD0594  [4]
 LDCM0278  AC19 HCT 116 C889(1.14)  LDD0595  [4]
 LDCM0280  AC20 HCT 116 C889(1.04)  LDD0597  [4]
 LDCM0281  AC21 HCT 116 C889(1.58)  LDD0598  [4]
 LDCM0282  AC22 HCT 116 C889(1.15)  LDD0599  [4]
 LDCM0283  AC23 HCT 116 C889(1.07)  LDD0600  [4]
 LDCM0284  AC24 HCT 116 C889(1.14)  LDD0601  [4]
 LDCM0285  AC25 HCT 116 C889(1.11)  LDD0602  [4]
 LDCM0286  AC26 HCT 116 C889(1.30)  LDD0603  [4]
 LDCM0287  AC27 HCT 116 C889(1.48)  LDD0604  [4]
 LDCM0288  AC28 HCT 116 C889(1.39)  LDD0605  [4]
 LDCM0289  AC29 HCT 116 C889(1.26)  LDD0606  [4]
 LDCM0291  AC30 HCT 116 C889(1.53)  LDD0608  [4]
 LDCM0292  AC31 HCT 116 C889(1.59)  LDD0609  [4]
 LDCM0293  AC32 HCT 116 C889(1.69)  LDD0610  [4]
 LDCM0294  AC33 HCT 116 C889(1.65)  LDD0611  [4]
 LDCM0295  AC34 HCT 116 C889(1.93)  LDD0612  [4]
 LDCM0308  AC46 HCT 116 C889(1.05)  LDD0625  [4]
 LDCM0309  AC47 HCT 116 C889(0.99)  LDD0626  [4]
 LDCM0310  AC48 HCT 116 C889(1.18)  LDD0627  [4]
 LDCM0311  AC49 HCT 116 C889(1.75)  LDD0628  [4]
 LDCM0313  AC50 HCT 116 C889(1.89)  LDD0630  [4]
 LDCM0314  AC51 HCT 116 C889(0.76)  LDD0631  [4]
 LDCM0315  AC52 HCT 116 C889(1.02)  LDD0632  [4]
 LDCM0316  AC53 HCT 116 C889(1.32)  LDD0633  [4]
 LDCM0317  AC54 HCT 116 C889(1.66)  LDD0634  [4]
 LDCM0318  AC55 HCT 116 C889(1.74)  LDD0635  [4]
 LDCM0319  AC56 HCT 116 C889(2.49)  LDD0636  [4]
 LDCM0320  AC57 HCT 116 C889(1.51)  LDD0637  [4]
 LDCM0321  AC58 HCT 116 C889(1.57)  LDD0638  [4]
 LDCM0322  AC59 HCT 116 C889(1.57)  LDD0639  [4]
 LDCM0324  AC60 HCT 116 C889(1.63)  LDD0641  [4]
 LDCM0325  AC61 HCT 116 C889(1.69)  LDD0642  [4]
 LDCM0326  AC62 HCT 116 C889(1.63)  LDD0643  [4]
 LDCM0327  AC63 HCT 116 C889(1.54)  LDD0644  [4]
 LDCM0328  AC64 HCT 116 C889(2.02)  LDD0645  [4]
 LDCM0329  AC65 HCT 116 C889(1.95)  LDD0646  [4]
 LDCM0330  AC66 HCT 116 C889(1.89)  LDD0647  [4]
 LDCM0331  AC67 HCT 116 C889(1.88)  LDD0648  [4]
 LDCM0365  AC98 HCT 116 C889(2.49)  LDD0682  [4]
 LDCM0366  AC99 HCT 116 C889(1.55)  LDD0683  [4]
 LDCM0374  CL105 HCT 116 C889(1.45)  LDD0691  [4]
 LDCM0375  CL106 HCT 116 C889(2.26)  LDD0692  [4]
 LDCM0376  CL107 HCT 116 C889(1.66)  LDD0693  [4]
 LDCM0377  CL108 HCT 116 C889(1.82)  LDD0694  [4]
 LDCM0378  CL109 HCT 116 C889(1.39)  LDD0695  [4]
 LDCM0380  CL110 HCT 116 C889(1.53)  LDD0697  [4]
 LDCM0381  CL111 HCT 116 C889(1.43)  LDD0698  [4]
 LDCM0382  CL112 HCT 116 C889(1.01)  LDD0699  [4]
 LDCM0383  CL113 HCT 116 C889(1.41)  LDD0700  [4]
 LDCM0384  CL114 HCT 116 C889(1.01)  LDD0701  [4]
 LDCM0385  CL115 HCT 116 C889(1.23)  LDD0702  [4]
 LDCM0386  CL116 HCT 116 C889(1.48)  LDD0703  [4]
 LDCM0392  CL121 HCT 116 C889(2.46)  LDD0709  [4]
 LDCM0393  CL122 HCT 116 C889(1.32)  LDD0710  [4]
 LDCM0394  CL123 HCT 116 C889(1.26)  LDD0711  [4]
 LDCM0395  CL124 HCT 116 C889(1.46)  LDD0712  [4]
 LDCM0396  CL125 HCT 116 C889(1.08)  LDD0713  [4]
 LDCM0397  CL126 HCT 116 C889(1.35)  LDD0714  [4]
 LDCM0398  CL127 HCT 116 C889(1.42)  LDD0715  [4]
 LDCM0399  CL128 HCT 116 C889(1.50)  LDD0716  [4]
 LDCM0403  CL16 HCT 116 C889(1.12)  LDD0720  [4]
 LDCM0404  CL17 HCT 116 C889(1.06)  LDD0721  [4]
 LDCM0405  CL18 HCT 116 C889(2.00)  LDD0722  [4]
 LDCM0406  CL19 HCT 116 C889(1.61)  LDD0723  [4]
 LDCM0408  CL20 HCT 116 C889(1.76)  LDD0725  [4]
 LDCM0409  CL21 HCT 116 C889(2.42)  LDD0726  [4]
 LDCM0410  CL22 HCT 116 C889(2.98)  LDD0727  [4]
 LDCM0411  CL23 HCT 116 C889(1.40)  LDD0728  [4]
 LDCM0412  CL24 HCT 116 C889(1.87)  LDD0729  [4]
 LDCM0413  CL25 HCT 116 C889(1.83)  LDD0730  [4]
 LDCM0414  CL26 HCT 116 C889(1.31)  LDD0731  [4]
 LDCM0415  CL27 HCT 116 C889(1.33)  LDD0732  [4]
 LDCM0416  CL28 HCT 116 C889(1.58)  LDD0733  [4]
 LDCM0417  CL29 HCT 116 C889(1.85)  LDD0734  [4]
 LDCM0419  CL30 HCT 116 C889(1.40)  LDD0736  [4]
 LDCM0420  CL31 HCT 116 C889(0.98)  LDD0737  [4]
 LDCM0421  CL32 HCT 116 C889(0.98)  LDD0738  [4]
 LDCM0422  CL33 HCT 116 C889(0.90)  LDD0739  [4]
 LDCM0423  CL34 HCT 116 C889(0.99)  LDD0740  [4]
 LDCM0424  CL35 HCT 116 C889(1.08)  LDD0741  [4]
 LDCM0425  CL36 HCT 116 C889(0.99)  LDD0742  [4]
 LDCM0426  CL37 HCT 116 C889(0.92)  LDD0743  [4]
 LDCM0428  CL39 HCT 116 C889(1.05)  LDD0745  [4]
 LDCM0430  CL40 HCT 116 C889(0.94)  LDD0747  [4]
 LDCM0431  CL41 HCT 116 C889(1.05)  LDD0748  [4]
 LDCM0432  CL42 HCT 116 C889(0.95)  LDD0749  [4]
 LDCM0433  CL43 HCT 116 C889(0.90)  LDD0750  [4]
 LDCM0434  CL44 HCT 116 C889(1.03)  LDD0751  [4]
 LDCM0435  CL45 HCT 116 C889(1.08)  LDD0752  [4]
 LDCM0436  CL46 HCT 116 C889(0.99)  LDD0753  [4]
 LDCM0437  CL47 HCT 116 C889(1.01)  LDD0754  [4]
 LDCM0438  CL48 HCT 116 C889(1.00)  LDD0755  [4]
 LDCM0439  CL49 HCT 116 C889(1.06)  LDD0756  [4]
 LDCM0441  CL50 HCT 116 C889(0.87)  LDD0758  [4]
 LDCM0442  CL51 HCT 116 C889(1.03)  LDD0759  [4]
 LDCM0443  CL52 HCT 116 C889(1.16)  LDD0760  [4]
 LDCM0444  CL53 HCT 116 C889(0.89)  LDD0761  [4]
 LDCM0445  CL54 HCT 116 C889(0.98)  LDD0762  [4]
 LDCM0446  CL55 HCT 116 C889(1.06)  LDD0763  [4]
 LDCM0447  CL56 HCT 116 C889(1.02)  LDD0764  [4]
 LDCM0448  CL57 HCT 116 C889(0.99)  LDD0765  [4]
 LDCM0449  CL58 HCT 116 C889(1.06)  LDD0766  [4]
 LDCM0450  CL59 HCT 116 C889(1.06)  LDD0767  [4]
 LDCM0452  CL60 HCT 116 C889(1.01)  LDD0769  [4]
 LDCM0453  CL61 HCT 116 C889(1.07)  LDD0770  [4]
 LDCM0454  CL62 HCT 116 C889(0.89)  LDD0771  [4]
 LDCM0455  CL63 HCT 116 C889(1.26)  LDD0772  [4]
 LDCM0456  CL64 HCT 116 C889(1.39)  LDD0773  [4]
 LDCM0457  CL65 HCT 116 C889(1.29)  LDD0774  [4]
 LDCM0458  CL66 HCT 116 C889(1.46)  LDD0775  [4]
 LDCM0459  CL67 HCT 116 C889(1.64)  LDD0776  [4]
 LDCM0460  CL68 HCT 116 C889(1.38)  LDD0777  [4]
 LDCM0461  CL69 HCT 116 C889(1.36)  LDD0778  [4]
 LDCM0463  CL70 HCT 116 C889(1.32)  LDD0780  [4]
 LDCM0464  CL71 HCT 116 C889(1.21)  LDD0781  [4]
 LDCM0465  CL72 HCT 116 C889(1.08)  LDD0782  [4]
 LDCM0466  CL73 HCT 116 C889(1.71)  LDD0783  [4]
 LDCM0467  CL74 HCT 116 C889(1.46)  LDD0784  [4]
 LDCM0625  F8 Ramos C421(1.45); C647(1.79); C571(1.26); C371(1.40)  LDD2187  [5]
 LDCM0572  Fragment10 Ramos C421(2.02); C647(1.09); C571(6.19); C371(0.89)  LDD2189  [5]
 LDCM0573  Fragment11 Ramos C421(3.85); C647(2.51); C571(2.82); C371(0.23)  LDD2190  [5]
 LDCM0574  Fragment12 Ramos C421(1.14); C647(1.18); C571(2.45); C371(0.64)  LDD2191  [5]
 LDCM0575  Fragment13 Ramos C421(1.09); C647(0.94); C571(1.01); C371(1.04)  LDD2192  [5]
 LDCM0576  Fragment14 Ramos C421(0.99); C647(0.86); C571(0.82); C371(0.95)  LDD2193  [5]
 LDCM0579  Fragment20 Ramos C421(1.38); C647(1.10); C571(2.78); C371(0.77)  LDD2194  [5]
 LDCM0580  Fragment21 Ramos C421(1.14); C647(0.99); C571(0.97); C371(0.60)  LDD2195  [5]
 LDCM0582  Fragment23 Ramos C421(1.39); C647(1.21); C571(1.24); C371(1.01)  LDD2196  [5]
 LDCM0578  Fragment27 Ramos C421(1.11); C647(1.08); C571(0.90); C371(0.87)  LDD2197  [5]
 LDCM0586  Fragment28 Ramos C421(0.59); C647(0.45); C571(0.89); C371(1.28)  LDD2198  [5]
 LDCM0588  Fragment30 Ramos C421(1.72); C647(1.19); C571(1.59); C371(0.87)  LDD2199  [5]
 LDCM0589  Fragment31 Ramos C421(1.20); C647(1.19); C571(0.99); C371(0.77)  LDD2200  [5]
 LDCM0590  Fragment32 Ramos C421(2.84); C647(2.12); C371(0.87)  LDD2201  [5]
 LDCM0468  Fragment33 HCT 116 C889(1.31)  LDD0785  [4]
 LDCM0596  Fragment38 Ramos C421(1.14); C647(1.38); C571(0.89); C371(0.42)  LDD2203  [5]
 LDCM0566  Fragment4 Ramos C421(0.97); C647(1.18); C571(2.02); C371(1.04)  LDD2184  [5]
 LDCM0427  Fragment51 HCT 116 C889(1.07)  LDD0744  [4]
 LDCM0610  Fragment52 Ramos C421(1.44); C647(1.46); C571(1.37)  LDD2204  [5]
 LDCM0614  Fragment56 Ramos C421(1.83); C647(1.76); C571(1.20); C371(1.37)  LDD2205  [5]
 LDCM0569  Fragment7 Ramos C421(1.23); C647(1.06); C571(3.98); C371(0.88)  LDD2186  [5]
 LDCM0571  Fragment9 Ramos C421(1.47); C647(1.11); C571(3.32); C371(0.86)  LDD2188  [5]
 LDCM0022  KB02 T cell C571(14.73)  LDD1703  [6]
 LDCM0023  KB03 Jurkat C371(5.13); C571(9.05)  LDD0209  [1]
 LDCM0024  KB05 MOLM-13 C371(1.68); C647(2.70); C421(1.25)  LDD3333  [7]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Protein-tyrosine kinase 6 (PTK6) Tyr protein kinase family Q13882
Exosome complex component RRP46 (EXOSC5) RNase PH family Q9NQT4
Other
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Placental protein 13-like (LGALS14) . Q8TCE9

References

1 Covalent Inhibition by a Natural Product-Inspired Latent Electrophile. J Am Chem Soc. 2023 May 24;145(20):11097-11109. doi: 10.1021/jacs.3c00598. Epub 2023 May 15.
2 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
3 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
4 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
5 Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry. Methods Enzymol. 2023;679:295-322. doi: 10.1016/bs.mie.2022.07.037. Epub 2022 Sep 7.
Mass spectrometry data entry: PXD027578
6 An Activity-Guided Map of Electrophile-Cysteine Interactions in Primary Human T Cells. Cell. 2020 Aug 20;182(4):1009-1026.e29. doi: 10.1016/j.cell.2020.07.001. Epub 2020 Jul 29.
7 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840