Details of the Target

General Information of Target

Target ID LDTP14212
Target Name Solute carrier family 12 member 7 (SLC12A7)
Gene Name SLC12A7
Gene ID 10723
Synonyms
KCC4; Solute carrier family 12 member 7; Electroneutral potassium-chloride cotransporter 4; K-Cl cotransporter 4
3D Structure
Download
2D Sequence (FASTA)
Download
3D Structure (PDB)
Download
Sequence
MMGEAAVAAGPCPLREDSFTRFSSQSNVYGLAGGAGGRGELLAATLKGKVLGFRYQDLRQ
KIRPVAKELQFNYIPVDAEIVSIDTFNKSPPKRGLVVGITFIKDSGDKGSPFLNIYCDYE
PGSEYNLDSIAQSCLNLELQFTPFQLCHAEVQVGDQLETVFLLSGNDPAIHLYKENEGLH
QFEEQPVENLFPELTNLTSSVLWLDVHNFPGTSRRLSALGCQSGYVRVAHVDQRSREVLQ
MWSVLQDGPISRVIVFSLSAAKETKDRPLQDEYSVLVASMLEPAVVYRDLLNRGLEDQLL
LPGSDQFDSVLCSLVTDVDLDGRPEVLVATYGQELLCYKYRGPESGLPEAQHGFHLLWQR
SFSSPLLAMAHVDLTGDGLQELAVVSLKGVHILQHSLIQASELVLTRLRHQVEQRRRRLQ
GLEDGAGAGPAENAAS
Target Type
Literature-reported
Target Bioclass
Transporter and channel
Family
SLC12A transporter family
Subcellular location
Cell membrane
Function
Mediates electroneutral potassium-chloride cotransport when activated by cell swelling. May mediate K(+) uptake into Deiters' cells in the cochlea and contribute to K(+) recycling in the inner ear. Important for the survival of cochlear outer and inner hair cells and the maintenance of the organ of Corti. May be required for basolateral Cl(-) extrusion in the kidney and contribute to renal acidification.
TTD ID
T83320
Uniprot ID
Q9Y666
DrugMap ID
TTU2PCD
Ensemble ID
ENST00000264930.10
HGNC ID
HGNC:10915

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
CCK81 SNV: p.G18R .
HDQP1 SNV: p.L490Q .
JURKAT SNV: p.Q1035Ter .
K562 SNV: p.P2S; p.V7M .
KMS12BM SNV: p.E26K .
LNCaP clone FGC SNV: p.A233T .
LU65 SNV: p.A277V DBIA    Probe Info 
MCC26 SNV: p.G328C .
PF382 SNV: p.P540L .
RD SNV: p.E1063K .
RL952 SNV: p.W380Ter .
SW403 SNV: p.G294C .
TOV21G SNV: p.W616C .
U937 SNV: p.T455N .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 6 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
HDSF-alk
 Probe Info  		1.68  LDD0197  [1]
OPA-S-S-alkyne
 Probe Info  		K393(3.46)  LDD3494  [2]
DBIA
 Probe Info  		C762(3.18)  LDD3310  [3]
Acrolein
 Probe Info  		N.A.  LDD0222  [4]
IA-alkyne
 Probe Info  		N.A.  LDD0162  [5]
ATP probe
 Probe Info  		N.A.  LDD0035  [6]
PAL-AfBPP Probe
Click To Hide/Show 1 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C161
 Probe Info  		10.56  LDD1841  [7]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0214  AC1 HEK-293T C762(0.98)  LDD1507  [8]
 LDCM0215  AC10 HEK-293T C762(0.87)  LDD1508  [8]
 LDCM0226  AC11 HEK-293T C762(0.85)  LDD1509  [8]
 LDCM0237  AC12 HEK-293T C762(1.14)  LDD1510  [8]
 LDCM0259  AC14 HEK-293T C762(0.91)  LDD1512  [8]
 LDCM0270  AC15 HEK-293T C762(0.83)  LDD1513  [8]
 LDCM0276  AC17 HEK-293T C762(1.04)  LDD1515  [8]
 LDCM0277  AC18 HEK-293T C762(0.93)  LDD1516  [8]
 LDCM0278  AC19 HEK-293T C762(0.93)  LDD1517  [8]
 LDCM0279  AC2 HEK-293T C762(1.12)  LDD1518  [8]
 LDCM0280  AC20 HEK-293T C762(1.02)  LDD1519  [8]
 LDCM0282  AC22 HEK-293T C762(1.26)  LDD1521  [8]
 LDCM0283  AC23 HEK-293T C762(1.06)  LDD1522  [8]
 LDCM0284  AC24 HEK-293T C762(1.00)  LDD1523  [8]
 LDCM0285  AC25 HEK-293T C762(1.00)  LDD1524  [8]
 LDCM0286  AC26 HEK-293T C762(1.12)  LDD1525  [8]
 LDCM0287  AC27 HEK-293T C762(1.05)  LDD1526  [8]
 LDCM0288  AC28 HEK-293T C762(0.97)  LDD1527  [8]
 LDCM0290  AC3 HEK-293T C762(0.98)  LDD1529  [8]
 LDCM0291  AC30 HEK-293T C762(1.12)  LDD1530  [8]
 LDCM0292  AC31 HEK-293T C762(0.95)  LDD1531  [8]
 LDCM0293  AC32 HEK-293T C762(0.87)  LDD1532  [8]
 LDCM0294  AC33 HEK-293T C762(1.27)  LDD1533  [8]
 LDCM0295  AC34 HEK-293T C762(1.07)  LDD1534  [8]
 LDCM0296  AC35 HEK-293T C762(0.87)  LDD1535  [8]
 LDCM0297  AC36 HEK-293T C762(1.03)  LDD1536  [8]
 LDCM0299  AC38 HEK-293T C762(0.98)  LDD1538  [8]
 LDCM0300  AC39 HEK-293T C762(0.85)  LDD1539  [8]
 LDCM0301  AC4 HEK-293T C762(1.02)  LDD1540  [8]
 LDCM0302  AC40 HEK-293T C762(1.12)  LDD1541  [8]
 LDCM0303  AC41 HEK-293T C762(1.05)  LDD1542  [8]
 LDCM0304  AC42 HEK-293T C762(1.02)  LDD1543  [8]
 LDCM0305  AC43 HEK-293T C762(0.97)  LDD1544  [8]
 LDCM0306  AC44 HEK-293T C762(1.09)  LDD1545  [8]
 LDCM0308  AC46 HEK-293T C762(0.91)  LDD1547  [8]
 LDCM0309  AC47 HEK-293T C762(0.96)  LDD1548  [8]
 LDCM0310  AC48 HEK-293T C762(0.97)  LDD1549  [8]
 LDCM0311  AC49 HEK-293T C762(0.86)  LDD1550  [8]
 LDCM0313  AC50 HEK-293T C762(0.93)  LDD1552  [8]
 LDCM0314  AC51 HEK-293T C762(0.96)  LDD1553  [8]
 LDCM0315  AC52 HEK-293T C762(1.08)  LDD1554  [8]
 LDCM0317  AC54 HEK-293T C762(0.98)  LDD1556  [8]
 LDCM0318  AC55 HEK-293T C762(0.84)  LDD1557  [8]
 LDCM0319  AC56 HEK-293T C762(0.90)  LDD1558  [8]
 LDCM0320  AC57 HEK-293T C762(1.15)  LDD1559  [8]
 LDCM0321  AC58 HEK-293T C762(0.94)  LDD1560  [8]
 LDCM0322  AC59 HEK-293T C762(0.86)  LDD1561  [8]
 LDCM0323  AC6 HEK-293T C762(1.04)  LDD1562  [8]
 LDCM0324  AC60 HEK-293T C762(0.90)  LDD1563  [8]
 LDCM0326  AC62 HEK-293T C762(1.02)  LDD1565  [8]
 LDCM0327  AC63 HEK-293T C762(0.84)  LDD1566  [8]
 LDCM0328  AC64 HEK-293T C762(0.84)  LDD1567  [8]
 LDCM0334  AC7 HEK-293T C762(0.98)  LDD1568  [8]
 LDCM0345  AC8 HEK-293T C762(1.08)  LDD1569  [8]
 LDCM0356  AKOS034007680 HEK-293T C762(0.97)  LDD1570  [8]
 LDCM0275  AKOS034007705 HEK-293T C762(1.03)  LDD1514  [8]
 LDCM0108  Chloroacetamide HeLa N.A.  LDD0222  [4]
 LDCM0367  CL1 HEK-293T C762(0.85)  LDD1571  [8]
 LDCM0368  CL10 HEK-293T C762(1.01)  LDD1572  [8]
 LDCM0369  CL100 HEK-293T C762(1.07)  LDD1573  [8]
 LDCM0370  CL101 HEK-293T C762(0.66)  LDD1574  [8]
 LDCM0372  CL103 HEK-293T C762(1.16)  LDD1576  [8]
 LDCM0373  CL104 HEK-293T C762(0.95)  LDD1577  [8]
 LDCM0374  CL105 HEK-293T C762(0.84)  LDD1578  [8]
 LDCM0376  CL107 HEK-293T C762(1.15)  LDD1580  [8]
 LDCM0377  CL108 HEK-293T C762(1.09)  LDD1581  [8]
 LDCM0378  CL109 HEK-293T C762(0.83)  LDD1582  [8]
 LDCM0379  CL11 HEK-293T C762(1.12)  LDD1583  [8]
 LDCM0381  CL111 HEK-293T C762(1.19)  LDD1585  [8]
 LDCM0382  CL112 HEK-293T C762(1.00)  LDD1586  [8]
 LDCM0383  CL113 HEK-293T C762(0.92)  LDD1587  [8]
 LDCM0385  CL115 HEK-293T C762(0.93)  LDD1589  [8]
 LDCM0386  CL116 HEK-293T C762(1.27)  LDD1590  [8]
 LDCM0387  CL117 HEK-293T C762(1.01)  LDD1591  [8]
 LDCM0389  CL119 HEK-293T C762(1.12)  LDD1593  [8]
 LDCM0390  CL12 HEK-293T C762(1.11)  LDD1594  [8]
 LDCM0391  CL120 HEK-293T C762(1.06)  LDD1595  [8]
 LDCM0392  CL121 HEK-293T C762(0.89)  LDD1596  [8]
 LDCM0394  CL123 HEK-293T C762(1.01)  LDD1598  [8]
 LDCM0395  CL124 HEK-293T C762(1.16)  LDD1599  [8]
 LDCM0396  CL125 HEK-293T C762(0.97)  LDD1600  [8]
 LDCM0398  CL127 HEK-293T C762(1.10)  LDD1602  [8]
 LDCM0399  CL128 HEK-293T C762(1.16)  LDD1603  [8]
 LDCM0400  CL13 HEK-293T C762(0.77)  LDD1604  [8]
 LDCM0402  CL15 HEK-293T C762(1.00)  LDD1606  [8]
 LDCM0403  CL16 HEK-293T C762(1.09)  LDD1607  [8]
 LDCM0404  CL17 HEK-293T C762(1.07)  LDD1608  [8]
 LDCM0405  CL18 HEK-293T C762(0.98)  LDD1609  [8]
 LDCM0406  CL19 HEK-293T C762(1.02)  LDD1610  [8]
 LDCM0408  CL20 HEK-293T C762(1.02)  LDD1612  [8]
 LDCM0410  CL22 HEK-293T C762(1.02)  LDD1614  [8]
 LDCM0411  CL23 HEK-293T C762(0.90)  LDD1615  [8]
 LDCM0412  CL24 HEK-293T C762(1.13)  LDD1616  [8]
 LDCM0413  CL25 HEK-293T C762(1.17)  LDD1617  [8]
 LDCM0415  CL27 HEK-293T C762(1.13)  LDD1619  [8]
 LDCM0416  CL28 HEK-293T C762(1.25)  LDD1620  [8]
 LDCM0417  CL29 HEK-293T C762(0.99)  LDD1621  [8]
 LDCM0418  CL3 HEK-293T C762(1.29)  LDD1622  [8]
 LDCM0419  CL30 HEK-293T C762(0.89)  LDD1623  [8]
 LDCM0420  CL31 HEK-293T C762(0.95)  LDD1624  [8]
 LDCM0421  CL32 HEK-293T C762(0.99)  LDD1625  [8]
 LDCM0423  CL34 HEK-293T C762(1.03)  LDD1627  [8]
 LDCM0424  CL35 HEK-293T C762(0.92)  LDD1628  [8]
 LDCM0425  CL36 HEK-293T C762(1.29)  LDD1629  [8]
 LDCM0426  CL37 HEK-293T C762(0.84)  LDD1630  [8]
 LDCM0428  CL39 HEK-293T C762(1.17)  LDD1632  [8]
 LDCM0429  CL4 HEK-293T C762(1.06)  LDD1633  [8]
 LDCM0430  CL40 HEK-293T C762(1.06)  LDD1634  [8]
 LDCM0431  CL41 HEK-293T C762(1.19)  LDD1635  [8]
 LDCM0432  CL42 HEK-293T C762(0.97)  LDD1636  [8]
 LDCM0433  CL43 HEK-293T C762(0.92)  LDD1637  [8]
 LDCM0434  CL44 HEK-293T C762(1.04)  LDD1638  [8]
 LDCM0436  CL46 HEK-293T C762(1.17)  LDD1640  [8]
 LDCM0437  CL47 HEK-293T C762(1.05)  LDD1641  [8]
 LDCM0438  CL48 HEK-293T C762(1.13)  LDD1642  [8]
 LDCM0439  CL49 HEK-293T C762(0.83)  LDD1643  [8]
 LDCM0440  CL5 HEK-293T C762(1.06)  LDD1644  [8]
 LDCM0443  CL52 HEK-293T C762(0.95)  LDD1646  [8]
 LDCM0444  CL53 HEK-293T C762(1.13)  LDD1647  [8]
 LDCM0445  CL54 HEK-293T C762(1.37)  LDD1648  [8]
 LDCM0446  CL55 HEK-293T C762(0.98)  LDD1649  [8]
 LDCM0447  CL56 HEK-293T C762(1.09)  LDD1650  [8]
 LDCM0449  CL58 HEK-293T C762(1.02)  LDD1652  [8]
 LDCM0450  CL59 HEK-293T C762(1.14)  LDD1653  [8]
 LDCM0451  CL6 HEK-293T C762(0.91)  LDD1654  [8]
 LDCM0452  CL60 HEK-293T C762(1.25)  LDD1655  [8]
 LDCM0453  CL61 HEK-293T C762(1.06)  LDD1656  [8]
 LDCM0455  CL63 HEK-293T C762(1.09)  LDD1658  [8]
 LDCM0456  CL64 HEK-293T C762(1.17)  LDD1659  [8]
 LDCM0457  CL65 HEK-293T C762(0.92)  LDD1660  [8]
 LDCM0458  CL66 HEK-293T C762(1.06)  LDD1661  [8]
 LDCM0459  CL67 HEK-293T C762(1.09)  LDD1662  [8]
 LDCM0460  CL68 HEK-293T C762(1.10)  LDD1663  [8]
 LDCM0462  CL7 HEK-293T C762(1.02)  LDD1665  [8]
 LDCM0463  CL70 HEK-293T C762(1.22)  LDD1666  [8]
 LDCM0464  CL71 HEK-293T C762(0.93)  LDD1667  [8]
 LDCM0465  CL72 HEK-293T C762(1.29)  LDD1668  [8]
 LDCM0466  CL73 HEK-293T C762(0.88)  LDD1669  [8]
 LDCM0469  CL76 HEK-293T C762(1.20)  LDD1672  [8]
 LDCM0470  CL77 HEK-293T C762(0.96)  LDD1673  [8]
 LDCM0471  CL78 HEK-293T C762(1.15)  LDD1674  [8]
 LDCM0472  CL79 HEK-293T C762(0.99)  LDD1675  [8]
 LDCM0473  CL8 HEK-293T C762(1.41)  LDD1676  [8]
 LDCM0474  CL80 HEK-293T C762(1.12)  LDD1677  [8]
 LDCM0476  CL82 HEK-293T C762(1.11)  LDD1679  [8]
 LDCM0477  CL83 HEK-293T C762(1.08)  LDD1680  [8]
 LDCM0478  CL84 HEK-293T C762(1.11)  LDD1681  [8]
 LDCM0479  CL85 HEK-293T C762(0.97)  LDD1682  [8]
 LDCM0481  CL87 HEK-293T C762(0.98)  LDD1684  [8]
 LDCM0482  CL88 HEK-293T C762(1.30)  LDD1685  [8]
 LDCM0483  CL89 HEK-293T C762(1.13)  LDD1686  [8]
 LDCM0485  CL90 HEK-293T C762(1.50)  LDD1688  [8]
 LDCM0486  CL91 HEK-293T C762(0.87)  LDD1689  [8]
 LDCM0487  CL92 HEK-293T C762(1.09)  LDD1690  [8]
 LDCM0489  CL94 HEK-293T C762(1.28)  LDD1692  [8]
 LDCM0490  CL95 HEK-293T C762(1.02)  LDD1693  [8]
 LDCM0491  CL96 HEK-293T C762(1.15)  LDD1694  [8]
 LDCM0492  CL97 HEK-293T C762(0.79)  LDD1695  [8]
 LDCM0494  CL99 HEK-293T C762(0.94)  LDD1697  [8]
 LDCM0495  E2913 HEK-293T C762(1.08)  LDD1698  [8]
 LDCM0468  Fragment33 HEK-293T C762(1.13)  LDD1671  [8]
 LDCM0022  KB02 769-P C762(2.26)  LDD2246  [3]
 LDCM0023  KB03 769-P C762(3.12)  LDD2663  [3]
 LDCM0024  KB05 COLO792 C762(3.18)  LDD3310  [3]
 LDCM0109  NEM HeLa N.A.  LDD0227  [4]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Transporter and channel
Click To Hide/Show 6 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Bax inhibitor 1 (TMBIM6) BI1 family P55061
Hepatic sodium/bile acid cotransporter (SLC10A1) Bile acid:sodium symporter (BASS) family Q14973
Sodium-dependent organic anion transporter (SLC10A6) Bile acid:sodium symporter (BASS) family Q3KNW5
Gap junction beta-1 protein (GJB1) Connexin family P08034
Membrane-spanning 4-domains subfamily A member 13 (MS4A13) MS4A family Q5J8X5
BOS complex subunit TMEM147 (TMEM147) TMEM147 family Q9BVK8
Transcription factor
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Homeobox protein orthopedia (OTP) Paired homeobox family Q5XKR4
GPCR
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Delta-type opioid receptor (OPRD1) G-protein coupled receptor 1 family P41143
G-protein coupled receptor 61 (GPR61) G-protein coupled receptor 1 family Q9BZJ8
Other
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Transmembrane protein 54 (TMEM54) TMEM54 family Q969K7
Protein YIPF6 (YIPF6) YIP1 family Q96EC8

The Drug(s) Related To This Target

Approved
Click To Hide/Show 1 Drug(s) Interacting with This Target
Drug Name Drug Type External ID
Potassium Chloride Small molecular drug DB00761
Investigative
Click To Hide/Show 1 Drug(s) Interacting with This Target
Drug Name Drug Type External ID
Dioa Small molecular drug D0K9KZ

References

1 Fatty Acyl Sulfonyl Fluoride as an Activity-Based Probe for Profiling Fatty Acid-Associated Proteins in Living Cells. Chembiochem. 2022 Feb 16;23(4):e202100628. doi: 10.1002/cbic.202100628. Epub 2021 Dec 30.
2 A chemical proteomics approach for global mapping of functional lysines on cell surface of living cell. Nat Commun. 2024 Apr 8;15(1):2997. doi: 10.1038/s41467-024-47033-w.
Mass spectrometry data entry: PXD042888
3 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
4 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
5 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
6 Comparison of Quantitative Mass Spectrometry Platforms for Monitoring Kinase ATP Probe Uptake in Lung Cancer. J Proteome Res. 2018 Jan 5;17(1):63-75. doi: 10.1021/acs.jproteome.7b00329. Epub 2017 Nov 22.
Mass spectrometry data entry: PXD006095 , PXD006096
7 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587
8 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402