Details of the Target

General Information of Target

Target ID LDTP13242
Target Name DNA polymerase lambda (POLL)
Gene Name POLL
Gene ID 27343
Synonyms
DNA polymerase lambda; Pol Lambda; EC 2.7.7.7; EC 4.2.99.-; DNA polymerase beta-2; Pol beta2; DNA polymerase kappa
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MDKYDDLGLEASKFIEDLNMYEASKDGLFRVDKGAGNNPEFEETRRVFATKMAKIHLQQQ
QQQLLQEETLPRGSRGPVNGGGRLGPQARWEVVGSKLTVDGAAKPPLAASTGAPGAVTTL
AAGQPPYPPQEQRSRPYLHGTRHGSQDCGSRESLATSEMSAFHQPGPCEDPSCLTHGDYY
DNLSLASPKWGDKPGVSPSIGLSVGSGWPSSPGSDPPLPKPCGDHPLNHRQLSLSSSRSS
EGSLGGQNSGIGGRSSEKPTGLWSTASSQRVSPGLPSPNLENGAPAVGPVQPRTPSVSAP
LALSCPRQGGLPRSNSGLGGEVSGVMSKPNVDPQPWFQDGPKSYLSSSAPSSSPAGLDGS
QQGAVPGLGPKPGCTDLGTGPKLSPTSLVHPVMSTLPELSCKEGPLGWSSDGSLGSVLLD
SPSSPRVRLPCQPLVPGPELRPSAAELKLEALTQRLEREMDAHPKADYFGACVKCSKGVF
GAGQACQAMGNLYHDTCFTCAACSRKLRGKAFYFVNGKVFCEEDFLYSGFQQSADRCFLC
GHLIMDMILQALGKSYHPGCFRCVICNECLDGVPFTVDSENKIYCVRDYHKVLAPKCAAC
GLPILPPEGSDETIRVVSMDRDYHVECYHCEDCGLELNDEDGHRCYPLEDHLFCHSCHVK
RLEKRPSSTALHQHHF
Target Bioclass
Enzyme
Family
DNA polymerase type-X family
Subcellular location
Nucleus
Function
DNA polymerase that functions in several pathways of DNA repair. Involved in base excision repair (BER) responsible for repair of lesions that give rise to abasic (AP) sites in DNA. Also contributes to DNA double-strand break repair by non-homologous end joining and homologous recombination. Has both template-dependent and template-independent (terminal transferase) DNA polymerase activities . Has also a 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity.
Uniprot ID
Q9UGP5
Ensemble ID
ENST00000299206.8
HGNC ID
HGNC:9184
ChEMBL ID
CHEMBL5367

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
DU145 SNV: p.A285T .
FTC133 SNV: p.S199G .
HCT15 SNV: p.L489M DBIA    Probe Info 
MEWO SNV: p.A405T DBIA    Probe Info 
NCIH196 SNV: p.R452L .
RL952 SNV: p.R478W DBIA    Probe Info 

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 1 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C241(1.24)  LDD0563  [1]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0214  AC1 HEK-293T C241(0.92)  LDD1507  [2]
 LDCM0246  AC128 HCT 116 C241(1.24)  LDD0563  [1]
 LDCM0247  AC129 HCT 116 C241(0.80)  LDD0564  [1]
 LDCM0249  AC130 HCT 116 C241(1.02)  LDD0566  [1]
 LDCM0250  AC131 HCT 116 C241(0.94)  LDD0567  [1]
 LDCM0251  AC132 HCT 116 C241(0.77)  LDD0568  [1]
 LDCM0252  AC133 HCT 116 C241(0.88)  LDD0569  [1]
 LDCM0253  AC134 HCT 116 C241(0.97)  LDD0570  [1]
 LDCM0254  AC135 HCT 116 C241(0.98)  LDD0571  [1]
 LDCM0255  AC136 HCT 116 C241(0.84)  LDD0572  [1]
 LDCM0256  AC137 HCT 116 C241(0.80)  LDD0573  [1]
 LDCM0257  AC138 HCT 116 C241(0.93)  LDD0574  [1]
 LDCM0258  AC139 HCT 116 C241(0.91)  LDD0575  [1]
 LDCM0260  AC140 HCT 116 C241(0.91)  LDD0577  [1]
 LDCM0261  AC141 HCT 116 C241(0.95)  LDD0578  [1]
 LDCM0262  AC142 HCT 116 C241(0.75)  LDD0579  [1]
 LDCM0276  AC17 HEK-293T C241(0.87)  LDD1515  [2]
 LDCM0285  AC25 HEK-293T C241(0.85)  LDD1524  [2]
 LDCM0294  AC33 HEK-293T C241(0.90)  LDD1533  [2]
 LDCM0303  AC41 HEK-293T C241(0.90)  LDD1542  [2]
 LDCM0308  AC46 HCT 116 C241(1.38)  LDD0625  [1]
 LDCM0309  AC47 HCT 116 C241(1.11)  LDD0626  [1]
 LDCM0310  AC48 HCT 116 C241(1.24)  LDD0627  [1]
 LDCM0311  AC49 HCT 116 C241(1.52)  LDD0628  [1]
 LDCM0313  AC50 HCT 116 C241(1.66)  LDD0630  [1]
 LDCM0314  AC51 HCT 116 C241(1.35)  LDD0631  [1]
 LDCM0315  AC52 HCT 116 C241(1.27)  LDD0632  [1]
 LDCM0316  AC53 HCT 116 C241(1.35)  LDD0633  [1]
 LDCM0317  AC54 HCT 116 C241(1.27)  LDD0634  [1]
 LDCM0318  AC55 HCT 116 C241(1.19)  LDD0635  [1]
 LDCM0319  AC56 HCT 116 C241(1.45)  LDD0636  [1]
 LDCM0320  AC57 HCT 116 C241(1.28)  LDD0637  [1]
 LDCM0321  AC58 HCT 116 C241(1.26)  LDD0638  [1]
 LDCM0322  AC59 HCT 116 C241(1.28)  LDD0639  [1]
 LDCM0324  AC60 HCT 116 C241(1.35)  LDD0641  [1]
 LDCM0325  AC61 HCT 116 C241(1.27)  LDD0642  [1]
 LDCM0326  AC62 HCT 116 C241(1.35)  LDD0643  [1]
 LDCM0327  AC63 HCT 116 C241(1.19)  LDD0644  [1]
 LDCM0328  AC64 HCT 116 C241(1.53)  LDD0645  [1]
 LDCM0329  AC65 HCT 116 C241(1.51)  LDD0646  [1]
 LDCM0330  AC66 HCT 116 C241(1.33)  LDD0647  [1]
 LDCM0331  AC67 HCT 116 C241(1.50)  LDD0648  [1]
 LDCM0332  AC68 HCT 116 C241(0.96)  LDD0649  [1]
 LDCM0333  AC69 HCT 116 C241(0.88)  LDD0650  [1]
 LDCM0335  AC70 HCT 116 C241(0.96)  LDD0652  [1]
 LDCM0336  AC71 HCT 116 C241(0.76)  LDD0653  [1]
 LDCM0337  AC72 HCT 116 C241(1.03)  LDD0654  [1]
 LDCM0338  AC73 HCT 116 C241(1.05)  LDD0655  [1]
 LDCM0339  AC74 HCT 116 C241(1.04)  LDD0656  [1]
 LDCM0340  AC75 HCT 116 C241(1.06)  LDD0657  [1]
 LDCM0341  AC76 HCT 116 C241(1.03)  LDD0658  [1]
 LDCM0342  AC77 HCT 116 C241(0.89)  LDD0659  [1]
 LDCM0343  AC78 HCT 116 C241(1.00)  LDD0660  [1]
 LDCM0344  AC79 HCT 116 C241(0.99)  LDD0661  [1]
 LDCM0346  AC80 HCT 116 C241(1.06)  LDD0663  [1]
 LDCM0347  AC81 HCT 116 C241(0.90)  LDD0664  [1]
 LDCM0348  AC82 HCT 116 C241(0.97)  LDD0665  [1]
 LDCM0356  AKOS034007680 HEK-293T C241(0.92)  LDD1570  [2]
 LDCM0367  CL1 HCT 116 C241(0.99)  LDD0684  [1]
 LDCM0368  CL10 HCT 116 C241(0.95)  LDD0685  [1]
 LDCM0379  CL11 HCT 116 C241(0.90)  LDD0696  [1]
 LDCM0390  CL12 HCT 116 C241(1.08)  LDD0707  [1]
 LDCM0392  CL121 HCT 116 C241(1.12)  LDD0709  [1]
 LDCM0393  CL122 HCT 116 C241(1.33)  LDD0710  [1]
 LDCM0394  CL123 HCT 116 C241(1.26)  LDD0711  [1]
 LDCM0395  CL124 HCT 116 C241(1.37)  LDD0712  [1]
 LDCM0396  CL125 HCT 116 C241(0.91)  LDD0713  [1]
 LDCM0397  CL126 HCT 116 C241(0.93)  LDD0714  [1]
 LDCM0398  CL127 HCT 116 C241(1.03)  LDD0715  [1]
 LDCM0399  CL128 HCT 116 C241(1.40)  LDD0716  [1]
 LDCM0400  CL13 HCT 116 C241(1.06)  LDD0717  [1]
 LDCM0401  CL14 HCT 116 C241(1.12)  LDD0718  [1]
 LDCM0402  CL15 HCT 116 C241(1.07)  LDD0719  [1]
 LDCM0403  CL16 HCT 116 C241(1.06)  LDD0720  [1]
 LDCM0404  CL17 HCT 116 C241(1.06)  LDD0721  [1]
 LDCM0405  CL18 HCT 116 C241(0.95)  LDD0722  [1]
 LDCM0406  CL19 HCT 116 C241(1.05)  LDD0723  [1]
 LDCM0407  CL2 HCT 116 C241(0.96)  LDD0724  [1]
 LDCM0408  CL20 HCT 116 C241(1.01)  LDD0725  [1]
 LDCM0409  CL21 HCT 116 C241(1.23)  LDD0726  [1]
 LDCM0410  CL22 HCT 116 C241(1.66)  LDD0727  [1]
 LDCM0411  CL23 HCT 116 C241(1.00)  LDD0728  [1]
 LDCM0412  CL24 HCT 116 C241(1.19)  LDD0729  [1]
 LDCM0413  CL25 HCT 116 C241(1.17)  LDD0730  [1]
 LDCM0414  CL26 HCT 116 C241(0.93)  LDD0731  [1]
 LDCM0415  CL27 HCT 116 C241(1.00)  LDD0732  [1]
 LDCM0416  CL28 HCT 116 C241(0.95)  LDD0733  [1]
 LDCM0417  CL29 HCT 116 C241(1.21)  LDD0734  [1]
 LDCM0418  CL3 HCT 116 C241(0.79)  LDD0735  [1]
 LDCM0419  CL30 HCT 116 C241(0.94)  LDD0736  [1]
 LDCM0420  CL31 HCT 116 C241(1.11)  LDD0737  [1]
 LDCM0421  CL32 HCT 116 C241(1.25)  LDD0738  [1]
 LDCM0422  CL33 HCT 116 C241(1.25)  LDD0739  [1]
 LDCM0423  CL34 HCT 116 C241(1.17)  LDD0740  [1]
 LDCM0424  CL35 HCT 116 C241(1.20)  LDD0741  [1]
 LDCM0425  CL36 HCT 116 C241(1.26)  LDD0742  [1]
 LDCM0426  CL37 HCT 116 C241(1.48)  LDD0743  [1]
 LDCM0428  CL39 HCT 116 C241(1.51)  LDD0745  [1]
 LDCM0429  CL4 HCT 116 C241(1.20)  LDD0746  [1]
 LDCM0430  CL40 HCT 116 C241(1.28)  LDD0747  [1]
 LDCM0431  CL41 HCT 116 C241(1.38)  LDD0748  [1]
 LDCM0432  CL42 HCT 116 C241(1.94)  LDD0749  [1]
 LDCM0433  CL43 HCT 116 C241(1.35)  LDD0750  [1]
 LDCM0434  CL44 HCT 116 C241(1.28)  LDD0751  [1]
 LDCM0435  CL45 HCT 116 C241(1.28)  LDD0752  [1]
 LDCM0436  CL46 HCT 116 C241(0.83)  LDD0753  [1]
 LDCM0437  CL47 HCT 116 C241(0.76)  LDD0754  [1]
 LDCM0438  CL48 HCT 116 C241(0.78)  LDD0755  [1]
 LDCM0439  CL49 HCT 116 C241(1.00)  LDD0756  [1]
 LDCM0440  CL5 HCT 116 C241(0.95)  LDD0757  [1]
 LDCM0441  CL50 HCT 116 C241(0.81)  LDD0758  [1]
 LDCM0442  CL51 HCT 116 C241(0.92)  LDD0759  [1]
 LDCM0443  CL52 HCT 116 C241(0.77)  LDD0760  [1]
 LDCM0444  CL53 HCT 116 C241(0.90)  LDD0761  [1]
 LDCM0445  CL54 HCT 116 C241(0.98)  LDD0762  [1]
 LDCM0446  CL55 HCT 116 C241(0.84)  LDD0763  [1]
 LDCM0447  CL56 HCT 116 C241(0.85)  LDD0764  [1]
 LDCM0448  CL57 HCT 116 C241(0.77)  LDD0765  [1]
 LDCM0449  CL58 HCT 116 C241(0.67)  LDD0766  [1]
 LDCM0450  CL59 HCT 116 C241(0.72)  LDD0767  [1]
 LDCM0451  CL6 HCT 116 C241(0.96)  LDD0768  [1]
 LDCM0452  CL60 HCT 116 C241(0.88)  LDD0769  [1]
 LDCM0453  CL61 HCT 116 C241(0.95)  LDD0770  [1]
 LDCM0454  CL62 HCT 116 C241(1.00)  LDD0771  [1]
 LDCM0455  CL63 HCT 116 C241(1.04)  LDD0772  [1]
 LDCM0456  CL64 HCT 116 C241(1.18)  LDD0773  [1]
 LDCM0457  CL65 HCT 116 C241(0.95)  LDD0774  [1]
 LDCM0458  CL66 HCT 116 C241(1.19)  LDD0775  [1]
 LDCM0459  CL67 HCT 116 C241(1.05)  LDD0776  [1]
 LDCM0460  CL68 HCT 116 C241(0.95)  LDD0777  [1]
 LDCM0461  CL69 HCT 116 C241(1.05)  LDD0778  [1]
 LDCM0462  CL7 HCT 116 C241(1.05)  LDD0779  [1]
 LDCM0463  CL70 HCT 116 C241(1.04)  LDD0780  [1]
 LDCM0464  CL71 HCT 116 C241(1.11)  LDD0781  [1]
 LDCM0465  CL72 HCT 116 C241(0.98)  LDD0782  [1]
 LDCM0466  CL73 HCT 116 C241(1.13)  LDD0783  [1]
 LDCM0467  CL74 HCT 116 C241(1.14)  LDD0784  [1]
 LDCM0469  CL76 HCT 116 C241(0.92)  LDD0786  [1]
 LDCM0470  CL77 HCT 116 C241(0.98)  LDD0787  [1]
 LDCM0471  CL78 HCT 116 C241(0.90)  LDD0788  [1]
 LDCM0472  CL79 HCT 116 C241(1.02)  LDD0789  [1]
 LDCM0473  CL8 HCT 116 C241(1.06)  LDD0790  [1]
 LDCM0474  CL80 HCT 116 C241(0.93)  LDD0791  [1]
 LDCM0475  CL81 HCT 116 C241(1.08)  LDD0792  [1]
 LDCM0476  CL82 HCT 116 C241(0.86)  LDD0793  [1]
 LDCM0477  CL83 HCT 116 C241(0.97)  LDD0794  [1]
 LDCM0478  CL84 HCT 116 C241(0.90)  LDD0795  [1]
 LDCM0479  CL85 HCT 116 C241(0.96)  LDD0796  [1]
 LDCM0480  CL86 HCT 116 C241(0.81)  LDD0797  [1]
 LDCM0481  CL87 HCT 116 C241(0.90)  LDD0798  [1]
 LDCM0482  CL88 HCT 116 C241(0.85)  LDD0799  [1]
 LDCM0483  CL89 HCT 116 C241(0.83)  LDD0800  [1]
 LDCM0484  CL9 HCT 116 C241(1.06)  LDD0801  [1]
 LDCM0485  CL90 HCT 116 C241(1.01)  LDD0802  [1]
 LDCM0468  Fragment33 HCT 116 C241(1.07)  LDD0785  [1]
 LDCM0427  Fragment51 HCT 116 C241(1.42)  LDD0744  [1]
 LDCM0022  KB02 A2058 C241(2.55)  LDD2253  [3]
 LDCM0023  KB03 A2058 C241(3.01)  LDD2670  [3]
 LDCM0024  KB05 COLO792 C241(6.27)  LDD3310  [3]

References

1 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
2 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
3 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840