Details of the Target

General Information of Target

Target ID LDTP11957
Target Name Protein O-mannose kinase (POMK)
Gene Name POMK
Gene ID 84197
Synonyms
SGK196; Protein O-mannose kinase; POMK; EC 2.7.1.183; Protein kinase-like protein SgK196; Sugen kinase 196
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MDKSGIDSLDHVTSDAVELANRSDNSSDSSLFKTQCIPYSPKGEKRNPIRKFVRTPESVH
ASDSSSDSSFEPIPLTIKAIFERFKNRKKRYKKKKKRRYQPTGRPRGRPEGRRNPIYSLI
DKKKQFRSRGSGFPFLESENEKNAPWRKILTFEQAVARGFFNYIEKLKYEHHLKESLKQM
NVGEDLENEDFDSRRYKFLDDDGSISPIEESTAEDEDATHLEDNECDIKLAGDSFIVSSE
FPVRLSVYLEEEDITEEAALSKKRATKAKNTGQRGLKM
Target Bioclass
Enzyme
Family
Protein kinase superfamily, Ser/Thr protein kinase family, STKL subfamily
Subcellular location
Endoplasmic reticulum membrane
Function
Protein O-mannose kinase that specifically mediates phosphorylation at the 6-position of an O-mannose of the trisaccharide (N-acetylgalactosamine (GalNAc)-beta-1,3-N-acetylglucosamine (GlcNAc)-beta-1,4-mannose) to generate phosphorylated O-mannosyl trisaccharide (N-acetylgalactosamine-beta-1,3-N-acetylglucosamine-beta-1,4-(phosphate-6-)mannose). Phosphorylated O-mannosyl trisaccharide is a carbohydrate structure present in alpha-dystroglycan (DAG1), which is required for binding laminin G-like domain-containing extracellular proteins with high affinity. Only shows kinase activity when the GalNAc-beta-3-GlcNAc-beta-terminus is linked to the 4-position of O-mannose, suggesting that this disaccharide serves as the substrate recognition motif.
Uniprot ID
Q9H5K3
Ensemble ID
ENST00000331373.10
HGNC ID
HGNC:26267

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 5 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C55(1.79)  LDD3323  [1]
BTD
 Probe Info  		C55(1.04)  LDD2094  [2]
5E-2FA
 Probe Info  		N.A.  LDD2235  [3]
m-APA
 Probe Info  		N.A.  LDD2231  [3]
IA-alkyne
 Probe Info  		C55(0.00); C203(0.00)  LDD0162  [4]
PAL-AfBPP Probe
Click To Hide/Show 4 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C091
 Probe Info  		25.63  LDD1782  [5]
C092
 Probe Info  		49.18  LDD1783  [5]
C094
 Probe Info  		55.72  LDD1785  [5]
OEA-DA
 Probe Info  		13.47  LDD0046  [6]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0214  AC1 HEK-293T C55(1.00); C203(1.05)  LDD1507  [7]
 LDCM0215  AC10 HEK-293T C55(1.01); C203(1.02)  LDD1508  [7]
 LDCM0226  AC11 HEK-293T C55(1.18); C203(1.06)  LDD1509  [7]
 LDCM0259  AC14 HEK-293T C55(1.02); C203(1.11)  LDD1512  [7]
 LDCM0270  AC15 HEK-293T C203(1.03)  LDD1513  [7]
 LDCM0276  AC17 HEK-293T C55(0.93); C203(1.00)  LDD1515  [7]
 LDCM0277  AC18 HEK-293T C55(1.11); C203(0.95)  LDD1516  [7]
 LDCM0278  AC19 HEK-293T C55(1.12); C203(1.24)  LDD1517  [7]
 LDCM0279  AC2 HEK-293T C55(0.97); C203(1.06)  LDD1518  [7]
 LDCM0281  AC21 HEK-293T C55(1.04); C203(1.04)  LDD1520  [7]
 LDCM0282  AC22 HEK-293T C55(1.15); C203(0.98)  LDD1521  [7]
 LDCM0283  AC23 HEK-293T C203(1.01)  LDD1522  [7]
 LDCM0284  AC24 HEK-293T C203(0.98)  LDD1523  [7]
 LDCM0285  AC25 HEK-293T C55(0.93); C203(0.93)  LDD1524  [7]
 LDCM0286  AC26 HEK-293T C55(0.99); C203(0.96)  LDD1525  [7]
 LDCM0287  AC27 HEK-293T C55(1.10); C203(1.08)  LDD1526  [7]
 LDCM0289  AC29 HEK-293T C55(1.03); C203(1.01)  LDD1528  [7]
 LDCM0290  AC3 HEK-293T C55(1.02); C203(1.05)  LDD1529  [7]
 LDCM0291  AC30 HEK-293T C55(1.04); C203(0.99)  LDD1530  [7]
 LDCM0292  AC31 HEK-293T C203(1.00)  LDD1531  [7]
 LDCM0293  AC32 HEK-293T C203(0.95)  LDD1532  [7]
 LDCM0294  AC33 HEK-293T C55(1.02); C203(1.11)  LDD1533  [7]
 LDCM0295  AC34 HEK-293T C55(1.08); C203(1.02)  LDD1534  [7]
 LDCM0296  AC35 HEK-293T C55(1.09); C203(1.06)  LDD1535  [7]
 LDCM0298  AC37 HEK-293T C55(1.14); C203(1.09)  LDD1537  [7]
 LDCM0299  AC38 HEK-293T C55(1.11); C203(1.09)  LDD1538  [7]
 LDCM0300  AC39 HEK-293T C203(1.08)  LDD1539  [7]
 LDCM0302  AC40 HEK-293T C203(1.04)  LDD1541  [7]
 LDCM0303  AC41 HEK-293T C55(1.02); C203(1.02)  LDD1542  [7]
 LDCM0304  AC42 HEK-293T C55(1.05); C203(1.04)  LDD1543  [7]
 LDCM0305  AC43 HEK-293T C55(1.11); C203(1.07)  LDD1544  [7]
 LDCM0307  AC45 HEK-293T C55(1.10); C203(1.12)  LDD1546  [7]
 LDCM0308  AC46 HEK-293T C55(1.14); C203(1.04)  LDD1547  [7]
 LDCM0309  AC47 HEK-293T C203(1.06)  LDD1548  [7]
 LDCM0310  AC48 HEK-293T C203(1.10)  LDD1549  [7]
 LDCM0311  AC49 HEK-293T C55(1.06); C203(1.01)  LDD1550  [7]
 LDCM0312  AC5 HEK-293T C55(1.03); C203(1.08)  LDD1551  [7]
 LDCM0313  AC50 HEK-293T C55(1.01); C203(1.02)  LDD1552  [7]
 LDCM0314  AC51 HEK-293T C55(1.11); C203(1.05)  LDD1553  [7]
 LDCM0316  AC53 HEK-293T C55(1.04); C203(1.07)  LDD1555  [7]
 LDCM0317  AC54 HEK-293T C55(1.04); C203(1.00)  LDD1556  [7]
 LDCM0318  AC55 HEK-293T C203(1.07)  LDD1557  [7]
 LDCM0319  AC56 HEK-293T C203(1.01)  LDD1558  [7]
 LDCM0320  AC57 HEK-293T C55(1.03); C203(1.00)  LDD1559  [7]
 LDCM0321  AC58 HEK-293T C55(1.05); C203(0.98)  LDD1560  [7]
 LDCM0322  AC59 HEK-293T C55(1.01); C203(1.03)  LDD1561  [7]
 LDCM0323  AC6 HEK-293T C55(1.08); C203(1.06)  LDD1562  [7]
 LDCM0325  AC61 HEK-293T C55(1.20); C203(1.12)  LDD1564  [7]
 LDCM0326  AC62 HEK-293T C55(0.99); C203(1.05)  LDD1565  [7]
 LDCM0327  AC63 HEK-293T C203(1.02)  LDD1566  [7]
 LDCM0328  AC64 HEK-293T C203(0.96)  LDD1567  [7]
 LDCM0334  AC7 HEK-293T C203(1.07)  LDD1568  [7]
 LDCM0345  AC8 HEK-293T C203(1.00)  LDD1569  [7]
 LDCM0248  AKOS034007472 HEK-293T C55(1.08); C203(1.09)  LDD1511  [7]
 LDCM0356  AKOS034007680 HEK-293T C55(1.08); C203(0.97)  LDD1570  [7]
 LDCM0275  AKOS034007705 HEK-293T C203(1.04)  LDD1514  [7]
 LDCM0368  CL10 HEK-293T C55(1.35); C203(1.28)  LDD1572  [7]
 LDCM0371  CL102 HEK-293T C55(1.17); C203(1.02)  LDD1575  [7]
 LDCM0375  CL106 HEK-293T C55(1.03); C203(1.03)  LDD1579  [7]
 LDCM0379  CL11 HEK-293T C203(1.17)  LDD1583  [7]
 LDCM0380  CL110 HEK-293T C55(1.14); C203(1.11)  LDD1584  [7]
 LDCM0384  CL114 HEK-293T C55(1.27); C203(1.10)  LDD1588  [7]
 LDCM0388  CL118 HEK-293T C55(1.02); C203(1.09)  LDD1592  [7]
 LDCM0390  CL12 HEK-293T C203(1.15)  LDD1594  [7]
 LDCM0393  CL122 HEK-293T C55(1.16); C203(1.06)  LDD1597  [7]
 LDCM0397  CL126 HEK-293T C55(1.10); C203(1.09)  LDD1601  [7]
 LDCM0401  CL14 HEK-293T C55(1.14); C203(1.06)  LDD1605  [7]
 LDCM0404  CL17 HEK-293T C55(1.34); C203(1.13)  LDD1608  [7]
 LDCM0405  CL18 HEK-293T C55(1.09); C203(0.99)  LDD1609  [7]
 LDCM0406  CL19 HEK-293T C55(1.21); C203(1.09)  LDD1610  [7]
 LDCM0407  CL2 HEK-293T C55(1.10); C203(1.17)  LDD1611  [7]
 LDCM0409  CL21 HEK-293T C55(1.38); C203(1.13)  LDD1613  [7]
 LDCM0410  CL22 HEK-293T C55(1.19); C203(1.16)  LDD1614  [7]
 LDCM0411  CL23 HEK-293T C203(1.19)  LDD1615  [7]
 LDCM0412  CL24 HEK-293T C203(1.07)  LDD1616  [7]
 LDCM0414  CL26 HEK-293T C55(0.95); C203(1.01)  LDD1618  [7]
 LDCM0417  CL29 HEK-293T C55(0.99); C203(0.99)  LDD1621  [7]
 LDCM0419  CL30 HEK-293T C55(1.07); C203(0.97)  LDD1623  [7]
 LDCM0420  CL31 HEK-293T C55(1.07); C203(1.13)  LDD1624  [7]
 LDCM0422  CL33 HEK-293T C55(1.26); C203(1.19)  LDD1626  [7]
 LDCM0423  CL34 HEK-293T C55(1.11); C203(1.10)  LDD1627  [7]
 LDCM0424  CL35 HEK-293T C203(1.11)  LDD1628  [7]
 LDCM0425  CL36 HEK-293T C203(1.04)  LDD1629  [7]
 LDCM0431  CL41 HEK-293T C55(1.11); C203(0.97)  LDD1635  [7]
 LDCM0432  CL42 HEK-293T C55(1.13); C203(1.03)  LDD1636  [7]
 LDCM0433  CL43 HEK-293T C55(1.15); C203(1.12)  LDD1637  [7]
 LDCM0435  CL45 HEK-293T C55(1.32); C203(1.11)  LDD1639  [7]
 LDCM0436  CL46 HEK-293T C55(1.17); C203(1.08)  LDD1640  [7]
 LDCM0437  CL47 HEK-293T C203(1.17)  LDD1641  [7]
 LDCM0438  CL48 HEK-293T C203(1.07)  LDD1642  [7]
 LDCM0440  CL5 HEK-293T C55(1.01); C203(1.05)  LDD1644  [7]
 LDCM0441  CL50 HEK-293T C55(1.13); C203(1.07)  LDD1645  [7]
 LDCM0444  CL53 HEK-293T C55(1.14); C203(1.22)  LDD1647  [7]
 LDCM0445  CL54 HEK-293T C55(1.29); C203(1.07)  LDD1648  [7]
 LDCM0446  CL55 HEK-293T C55(1.13); C203(1.09)  LDD1649  [7]
 LDCM0448  CL57 HEK-293T C55(1.50); C203(1.09)  LDD1651  [7]
 LDCM0449  CL58 HEK-293T C55(1.23); C203(1.16)  LDD1652  [7]
 LDCM0450  CL59 HEK-293T C203(1.17)  LDD1653  [7]
 LDCM0451  CL6 HEK-293T C55(1.12); C203(1.14)  LDD1654  [7]
 LDCM0452  CL60 HEK-293T C203(1.18)  LDD1655  [7]
 LDCM0454  CL62 HEK-293T C55(1.07); C203(1.03)  LDD1657  [7]
 LDCM0457  CL65 HEK-293T C55(1.06); C203(0.94)  LDD1660  [7]
 LDCM0458  CL66 HEK-293T C55(1.08); C203(0.97)  LDD1661  [7]
 LDCM0459  CL67 HEK-293T C55(1.09); C203(1.14)  LDD1662  [7]
 LDCM0461  CL69 HEK-293T C55(1.28); C203(1.12)  LDD1664  [7]
 LDCM0462  CL7 HEK-293T C55(1.18); C203(1.07)  LDD1665  [7]
 LDCM0463  CL70 HEK-293T C55(1.15); C203(1.00)  LDD1666  [7]
 LDCM0464  CL71 HEK-293T C203(1.20)  LDD1667  [7]
 LDCM0465  CL72 HEK-293T C203(1.09)  LDD1668  [7]
 LDCM0467  CL74 HEK-293T C55(0.97); C203(1.00)  LDD1670  [7]
 LDCM0470  CL77 HEK-293T C55(1.12); C203(1.12)  LDD1673  [7]
 LDCM0471  CL78 HEK-293T C55(1.09); C203(1.08)  LDD1674  [7]
 LDCM0472  CL79 HEK-293T C55(1.14); C203(1.07)  LDD1675  [7]
 LDCM0475  CL81 HEK-293T C55(1.24); C203(1.08)  LDD1678  [7]
 LDCM0476  CL82 HEK-293T C55(1.22); C203(1.13)  LDD1679  [7]
 LDCM0477  CL83 HEK-293T C203(1.26)  LDD1680  [7]
 LDCM0478  CL84 HEK-293T C203(1.20)  LDD1681  [7]
 LDCM0480  CL86 HEK-293T C55(1.16); C203(1.03)  LDD1683  [7]
 LDCM0483  CL89 HEK-293T C55(1.00); C203(1.03)  LDD1686  [7]
 LDCM0484  CL9 HEK-293T C55(1.27); C203(1.17)  LDD1687  [7]
 LDCM0485  CL90 HEK-293T C55(1.25); C203(1.30)  LDD1688  [7]
 LDCM0486  CL91 HEK-293T C55(1.18); C203(1.05)  LDD1689  [7]
 LDCM0488  CL93 HEK-293T C55(1.10); C203(1.09)  LDD1691  [7]
 LDCM0489  CL94 HEK-293T C55(1.12); C203(1.10)  LDD1692  [7]
 LDCM0490  CL95 HEK-293T C203(1.24)  LDD1693  [7]
 LDCM0491  CL96 HEK-293T C203(1.19)  LDD1694  [7]
 LDCM0493  CL98 HEK-293T C55(1.00); C203(1.01)  LDD1696  [7]
 LDCM0427  Fragment51 HEK-293T C55(1.17); C203(0.99)  LDD1631  [7]
 LDCM0022  KB02 A101D C55(1.97)  LDD2250  [1]
 LDCM0023  KB03 A101D C55(1.74)  LDD2667  [1]
 LDCM0024  KB05 SKMEL24 C55(1.79)  LDD3323  [1]
 LDCM0501  Nucleophilic fragment 13b MDA-MB-231 C55(1.04)  LDD2094  [2]
 LDCM0512  Nucleophilic fragment 19a MDA-MB-231 C55(1.69)  LDD2105  [2]
 LDCM0559  Nucleophilic fragment 9b MDA-MB-231 C55(1.39)  LDD2153  [2]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 3 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Protein O-linked-mannose beta-1,2-N-acetylglucosaminyltransferase 1 (POMGNT1) Glycosyltransferase 13 family Q8WZA1
Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 3 (B3GAT3) Glycosyltransferase 43 family O94766
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3A (STT3A) STT3 family P46977
Transporter and channel
Click To Hide/Show 3 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Membrane-spanning 4-domains subfamily A member 13 (MS4A13) MS4A family Q5J8X5
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1 (RPN1) OST1 family P04843
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2 (RPN2) SWP1 family P04844
Other
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Immediate early response 3-interacting protein 1 (IER3IP1) YOS1 family Q9Y5U9

References

1 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
2 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
3 Global profiling of functional histidines in live cells using small-molecule photosensitizer and chemical probe relay labelling. Nat Chem. 2024 Jun 4. doi: 10.1038/s41557-024-01545-6. Online ahead of print.
Mass spectrometry data entry: PXD042377
4 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
5 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587
6 Mapping Protein Targets of Bioactive Small Molecules Using Lipid-Based Chemical Proteomics. ACS Chem Biol. 2017 Oct 20;12(10):2671-2681. doi: 10.1021/acschembio.7b00581. Epub 2017 Sep 20.
Mass spectrometry data entry: PXD007570
7 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402