Details of the Target

General Information of Target

Target ID LDTP09976
Target Name Sarcoplasmic/endoplasmic reticulum calcium ATPase 3 (ATP2A3)
Gene Name ATP2A3
Gene ID 489
Synonyms
Sarcoplasmic/endoplasmic reticulum calcium ATPase 3; SERCA3; SR Ca(2+)-ATPase 3; EC 7.2.2.10; Calcium pump 3
3D Structure
Download
2D Sequence (FASTA)
Download
3D Structure (PDB)
Download
Sequence
MPDPAKSAPAPKKGSKKAVTKAQKKDGKKRKRSRKESYSVYVYKVLKQVHPDTGISSKAM
GIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLPGELAKHAVSEGTKAVT
KYTSSK
Target Bioclass
Enzyme
Family
Cation transport ATPase (P-type) (TC 3.A.3) family, Type IIA subfamily
Subcellular location
Nucleus membrane
Function
This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium. Transports calcium ions from the cytosol into the sarcoplasmic/endoplasmic reticulum lumen. Contributes to calcium sequestration involved in muscular excitation/contraction.
Uniprot ID
Q93084
Ensemble ID
ENST00000309890.11
HGNC ID
HGNC:813
ChEMBL ID
CHEMBL2401

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 15 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C636(4.54); C364(1.70)  LDD3310  [1]
SAHA-CA-4PAP
 Probe Info  		4.20  LDD0269  [2]
Johansson_61
 Probe Info  		_(5.64)  LDD1485  [3]
AHL-Pu-1
 Probe Info  		C447(2.03)  LDD0168  [4]
4-Iodoacetamidophenylacetylene
 Probe Info  		C561(0.00); C581(0.00); C614(0.00); C498(0.00)  LDD0038  [5]
IA-alkyne
 Probe Info  		C471(0.00); C614(0.00); C561(0.00); C581(0.00)  LDD0036  [5]
IPIAA_H
 Probe Info  		N.A.  LDD0030  [6]
IPIAA_L
 Probe Info  		N.A.  LDD0031  [6]
Lodoacetamide azide
 Probe Info  		C561(0.00); C614(0.00); C498(0.00); C581(0.00)  LDD0037  [5]
Compound 10
 Probe Info  		N.A.  LDD2216  [7]
Acrolein
 Probe Info  		C447(0.00); C364(0.00); C636(0.00)  LDD0217  [8]
Cinnamaldehyde
 Probe Info  		C364(0.00); C447(0.00)  LDD0220  [8]
Crotonaldehyde
 Probe Info  		C364(0.00); C447(0.00)  LDD0219  [8]
Methacrolein
 Probe Info  		C447(0.00); C364(0.00)  LDD0218  [8]
NAIA_5
 Probe Info  		C614(0.00); C498(0.00); C636(0.00)  LDD2223  [9]
PAL-AfBPP Probe
Click To Hide/Show 6 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
FFF probe11
 Probe Info  		20.00  LDD0472  [10]
FFF probe13
 Probe Info  		10.34  LDD0476  [10]
FFF probe3
 Probe Info  		20.00  LDD0465  [10]
STS-1
 Probe Info  		N.A.  LDD0136  [11]
STS-2
 Probe Info  		N.A.  LDD0138  [11]
VE-P
 Probe Info  		N.A.  LDD0396  [12]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0025  4SU-RNA HEK-293T C447(2.03)  LDD0168  [4]
 LDCM0108  Chloroacetamide HeLa C447(0.00); C364(0.00); C636(0.00)  LDD0222  [8]
 LDCM0632  CL-Sc Hep-G2 C636(2.11); C447(0.97)  LDD2227  [9]
 LDCM0634  CY-0357 Hep-G2 C581(0.98)  LDD2228  [9]
 LDCM0625  F8 Ramos C636(0.53); C364(1.65); C447(0.77); C471(0.98)  LDD2187  [13]
 LDCM0572  Fragment10 Ramos C636(3.22); C364(0.39); C447(0.97); C561(0.31)  LDD2189  [13]
 LDCM0573  Fragment11 Ramos C636(0.36); C364(0.48); C447(0.15); C561(0.26)  LDD2190  [13]
 LDCM0574  Fragment12 Ramos C636(7.00); C364(0.66); C447(1.48); C561(0.63)  LDD2191  [13]
 LDCM0575  Fragment13 Ramos C636(1.46); C364(0.76); C447(0.96); C561(1.16)  LDD2192  [13]
 LDCM0576  Fragment14 Ramos C636(2.29); C364(1.89); C447(2.52); C561(0.70)  LDD2193  [13]
 LDCM0579  Fragment20 Ramos C636(7.69); C364(0.75); C447(3.12); C561(0.80)  LDD2194  [13]
 LDCM0580  Fragment21 Ramos C636(1.79); C364(0.67); C447(1.37); C471(0.97)  LDD2195  [13]
 LDCM0582  Fragment23 Ramos C636(2.20); C364(0.54); C447(1.43); C561(0.29)  LDD2196  [13]
 LDCM0578  Fragment27 Ramos C636(1.26); C364(0.65); C447(0.96); C471(1.00)  LDD2197  [13]
 LDCM0586  Fragment28 Ramos C636(0.93); C364(0.56); C447(1.39); C471(0.38)  LDD2198  [13]
 LDCM0588  Fragment30 Ramos C636(2.25); C364(0.70); C447(0.78); C561(2.17)  LDD2199  [13]
 LDCM0589  Fragment31 Ramos C636(1.91); C364(0.78); C447(1.29); C561(1.61)  LDD2200  [13]
 LDCM0590  Fragment32 Ramos C636(2.79); C364(0.41); C447(0.89); C561(0.46)  LDD2201  [13]
 LDCM0468  Fragment33 Ramos C636(1.18); C364(0.50); C447(0.67); C561(2.96)  LDD2202  [13]
 LDCM0596  Fragment38 Ramos C636(2.14); C364(0.78); C447(0.72); C471(1.17)  LDD2203  [13]
 LDCM0566  Fragment4 Ramos C636(1.87); C364(0.91); C447(1.67); C561(1.43)  LDD2184  [13]
 LDCM0610  Fragment52 Ramos C636(1.66); C364(0.83); C447(0.65); C561(0.36)  LDD2204  [13]
 LDCM0614  Fragment56 Ramos C636(1.47); C364(0.76); C447(0.75); C561(2.29)  LDD2205  [13]
 LDCM0617  Fragment63-S Jurkat _(20.00)  LDD1490  [3]
 LDCM0569  Fragment7 Jurkat _(5.64)  LDD1485  [3]
 LDCM0571  Fragment9 Ramos C636(9.80); C364(0.65); C447(3.05); C561(0.55)  LDD2188  [13]
 LDCM0107  IAA HeLa C364(0.00); C636(0.00); C447(0.00)  LDD0221  [8]
 LDCM0022  KB02 Ramos C636(1.37); C364(0.79); C447(1.05); C561(0.77)  LDD2182  [13]
 LDCM0023  KB03 Ramos C636(4.34); C364(1.24); C447(1.74); C561(1.15)  LDD2183  [13]
 LDCM0024  KB05 COLO792 C636(4.54); C364(1.70)  LDD3310  [1]
 LDCM0109  NEM HeLa N.A.  LDD0232  [8]
 LDCM0131  RA190 MM1.R C581(1.55)  LDD0304  [14]
 LDCM0096  SAHA K562 4.20  LDD0269  [2]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
TGF-beta receptor type-2 (TGFBR2) TKL Ser/Thr protein kinase family P37173

References

1 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
2 Streamlined Target Deconvolution Approach Utilizing a Single Photoreactive Chloroalkane Capture Tag. ACS Chem Biol. 2021 Feb 19;16(2):404-413. doi: 10.1021/acschembio.0c00987. Epub 2021 Feb 5.
3 Proteome-wide covalent ligand discovery in native biological systems. Nature. 2016 Jun 23;534(7608):570-4. doi: 10.1038/nature18002. Epub 2016 Jun 15.
4 Chemoproteomic capture of RNA binding activity in living cells. Nat Commun. 2023 Oct 7;14(1):6282. doi: 10.1038/s41467-023-41844-z.
Mass spectrometry data entry: PXD044625
5 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
6 SP3-Enabled Rapid and High Coverage Chemoproteomic Identification of Cell-State-Dependent Redox-Sensitive Cysteines. Mol Cell Proteomics. 2022 Apr;21(4):100218. doi: 10.1016/j.mcpro.2022.100218. Epub 2022 Feb 25.
Mass spectrometry data entry: PXD029500 , PXD031647
7 Multiplexed CuAAC Suzuki-Miyaura Labeling for Tandem Activity-Based Chemoproteomic Profiling. Anal Chem. 2021 Feb 2;93(4):2610-2618. doi: 10.1021/acs.analchem.0c04726. Epub 2021 Jan 20.
Mass spectrometry data entry: PXD022279
8 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
9 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
10 Ligand and Target Discovery by Fragment-Based Screening in Human Cells. Cell. 2017 Jan 26;168(3):527-541.e29. doi: 10.1016/j.cell.2016.12.029. Epub 2017 Jan 19.
11 Design and synthesis of minimalist terminal alkyne-containing diazirine photo-crosslinkers and their incorporation into kinase inhibitors for cell- and tissue-based proteome profiling. Angew Chem Int Ed Engl. 2013 Aug 12;52(33):8551-6. doi: 10.1002/anie.201300683. Epub 2013 Jun 10.
12 Pharmacological Targeting of Vacuolar H(+)-ATPase via Subunit V1G Combats Multidrug-Resistant Cancer. Cell Chem Biol. 2020 Nov 19;27(11):1359-1370.e8. doi: 10.1016/j.chembiol.2020.06.011. Epub 2020 Jul 9.
13 Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry. Methods Enzymol. 2023;679:295-322. doi: 10.1016/bs.mie.2022.07.037. Epub 2022 Sep 7.
Mass spectrometry data entry: PXD027578
14 Physical and Functional Analysis of the Putative Rpn13 Inhibitor RA190. Cell Chem Biol. 2020 Nov 19;27(11):1371-1382.e6. doi: 10.1016/j.chembiol.2020.08.007. Epub 2020 Aug 27.