Details of the Target

General Information of Target

Target ID LDTP09777
Target Name T-complex protein 1 subunit zeta-2 (CCT6B)
Gene Name CCT6B
Gene ID 10693
Synonyms
T-complex protein 1 subunit zeta-2; TCP-1-zeta-2; CCT-zeta-2; CCT-zeta-like; TCP-1-zeta-like; Testis-specific Tcp20; Testis-specific protein TSA303
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MAEAHQAVAFQFTVTPDGVDFRLSREALKHVYLSGINSWKKRLIRIKNGILRGVYPGSPT
SWLVVIMATVGSSFCNVDISLGLVSCIQRCLPQGCGPYQTPQTRALLSMAIFSTGVWVTG
IFFFRQTLKLLLCYHGWMFEMHGKTSNLTRIWAMCIRLLSSRHPMLYSFQTSLPKLPVPR
VSATIQRYLESVRPLLDDEEYYRMELLAKEFQDKTAPRLQKYLVLKSWWASNYVSDWWEE
YIYLRGRSPLMVNSNYYVMDLVLIKNTDVQAARLGNIIHAMIMYRRKLDREEIKPVMALG
IVPMCSYQMERMFNTTRIPGKDTDVLQHLSDSRHVAVYHKGRFFKLWLYEGARLLKPQDL
EMQFQRILDDPSPPQPGEEKLAALTAGGRVEWAQARQAFFSSGKNKAALEAIERAAFFVA
LDEESYSYDPEDEASLSLYGKALLHGNCYNRWFDKSFTLISFKNGQLGLNAEHAWADAPI
IGHLWEFVLGTDSFHLGYTETGHCLGKPNPALAPPTRLQWDIPKQCQAVIESSYQVAKAL
ADDVELYCFQFLPFGKGLIKKCRTSPDAFVQIALQLAHFRDRGKFCLTYEASMTRMFREG
RTETVRSCTSESTAFVQAMMEGSHTKADLRDLFQKAAKKHQNMYRLAMTGAGIDRHLFCL
YLVSKYLGVSSPFLAEVLSEPWRLSTSQIPQSQIRMFDPEQHPNHLGAGGGFGPVADDGY
GVSYMIAGENTIFFHISSKFSSSETNAQRFGNHIRKALLDIADLFQVPKAYS
Target Bioclass
Other
Family
TCP-1 chaperonin family
Subcellular location
Cytoplasm
Function Component of the chaperonin-containing T-complex (TRiC), a molecular chaperone complex that assists the folding of proteins upon ATP hydrolysis.
Uniprot ID
Q92526
Ensemble ID
ENST00000314144.10
HGNC ID
HGNC:1621

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
HT115 SNV: p.V271A .
HUPT4 SNV: p.N293Y DBIA    Probe Info 
LN229 SNV: p.Y457N .
LS123 SNV: p.R434P .
LS180 Deletion: p.N400MfsTer30 DBIA    Probe Info 
NCIH1155 SNV: p.A126T .
WM115 SNV: p.P448S .
WM2664 Insertion: p.E257RfsTer7
SNV: p.P448S		 .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 8 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
TH216
 Probe Info  		Y109(13.15)  LDD0259  [1]
YN-4
 Probe Info  		100.00  LDD0445  [2]
DBIA
 Probe Info  		C508(4.56)  LDD3313  [3]
HHS-482
 Probe Info  		Y109(0.77)  LDD0285  [4]
HHS-465
 Probe Info  		Y109(10.00)  LDD2237  [5]
Lodoacetamide azide
 Probe Info  		N.A.  LDD0037  [6]
Acrolein
 Probe Info  		N.A.  LDD0217  [7]
Methacrolein
 Probe Info  		N.A.  LDD0218  [7]
PAL-AfBPP Probe
Click To Hide/Show 3 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
STS-1
 Probe Info  		N.A.  LDD0137  [8]
STS-2
 Probe Info  		N.A.  LDD0138  [8]
DA-2
 Probe Info  		N.A.  LDD0070  [9]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0108  Chloroacetamide HeLa N.A.  LDD0222  [7]
 LDCM0371  CL102 HEK-293T C233(0.86); C282(0.94)  LDD1575  [10]
 LDCM0375  CL106 HEK-293T C233(1.06); C282(0.98)  LDD1579  [10]
 LDCM0380  CL110 HEK-293T C233(0.94); C282(1.06)  LDD1584  [10]
 LDCM0384  CL114 HEK-293T C233(0.92); C282(1.21)  LDD1588  [10]
 LDCM0388  CL118 HEK-293T C233(0.98); C282(1.11)  LDD1592  [10]
 LDCM0393  CL122 HEK-293T C233(0.83); C282(0.83)  LDD1597  [10]
 LDCM0397  CL126 HEK-293T C233(0.91); C282(0.94)  LDD1601  [10]
 LDCM0401  CL14 HEK-293T C233(1.15); C282(0.84)  LDD1605  [10]
 LDCM0407  CL2 HEK-293T C233(1.16); C282(0.86)  LDD1611  [10]
 LDCM0414  CL26 HEK-293T C233(1.00); C282(0.96)  LDD1618  [10]
 LDCM0441  CL50 HEK-293T C233(0.96); C282(0.93)  LDD1645  [10]
 LDCM0454  CL62 HEK-293T C233(1.04); C282(0.92)  LDD1657  [10]
 LDCM0467  CL74 HEK-293T C233(1.12); C282(0.95)  LDD1670  [10]
 LDCM0480  CL86 HEK-293T C233(0.86); C282(0.98)  LDD1683  [10]
 LDCM0493  CL98 HEK-293T C233(0.93); C282(1.16)  LDD1696  [10]
 LDCM0427  Fragment51 HEK-293T C233(1.01); C282(0.84)  LDD1631  [10]
 LDCM0107  IAA HeLa H214(0.00); H115(0.00)  LDD0221  [7]
 LDCM0123  JWB131 DM93 Y109(0.77)  LDD0285  [4]
 LDCM0124  JWB142 DM93 Y109(0.35)  LDD0286  [4]
 LDCM0125  JWB146 DM93 Y109(0.98)  LDD0287  [4]
 LDCM0126  JWB150 DM93 Y109(4.37)  LDD0288  [4]
 LDCM0127  JWB152 DM93 Y109(1.18)  LDD0289  [4]
 LDCM0128  JWB198 DM93 Y109(1.20)  LDD0290  [4]
 LDCM0129  JWB202 DM93 Y109(0.30)  LDD0291  [4]
 LDCM0130  JWB211 DM93 Y109(0.85)  LDD0292  [4]
 LDCM0022  KB02 A-172 C508(1.29)  LDD2251  [3]
 LDCM0023  KB03 42-MG-BA C508(2.51)  LDD2661  [3]
 LDCM0024  KB05 Hs 936.T C508(4.56)  LDD3313  [3]
 LDCM0109  NEM HeLa H115(0.00); H214(0.00)  LDD0223  [7]

References

1 Chemoproteomic profiling of kinases in live cells using electrophilic sulfonyl triazole probes. Chem Sci. 2021 Jan 21;12(9):3295-3307. doi: 10.1039/d0sc06623k.
2 Ynamide Electrophile for the Profiling of Ligandable Carboxyl Residues in Live Cells and the Development of New Covalent Inhibitors. J Med Chem. 2022 Aug 11;65(15):10408-10418. doi: 10.1021/acs.jmedchem.2c00272. Epub 2022 Jul 26.
3 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
4 Chemoproteomic profiling of kinases in live cells using electrophilic sulfonyl triazole probes. Chem Sci. 2021 Jan 21;12(9):3295-3307. doi: 10.1039/d0sc06623k.
5 Global targeting of functional tyrosines using sulfur-triazole exchange chemistry. Nat Chem Biol. 2020 Feb;16(2):150-159. doi: 10.1038/s41589-019-0404-5. Epub 2019 Nov 25.
6 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
7 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
8 Design and synthesis of minimalist terminal alkyne-containing diazirine photo-crosslinkers and their incorporation into kinase inhibitors for cell- and tissue-based proteome profiling. Angew Chem Int Ed Engl. 2013 Aug 12;52(33):8551-6. doi: 10.1002/anie.201300683. Epub 2013 Jun 10.
9 Cell-based proteome profiling of potential dasatinib targets by use of affinity-based probes. J Am Chem Soc. 2012 Feb 15;134(6):3001-14. doi: 10.1021/ja208518u. Epub 2012 Feb 1.
10 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402