Details of the Target

General Information of Target

Target ID LDTP09484
Target Name Protein phosphatase Slingshot homolog 3 (SSH3)
Gene Name SSH3
Gene ID 54961
Synonyms
SSH3L; Protein phosphatase Slingshot homolog 3; EC 3.1.3.16; EC 3.1.3.48; SSH-like protein 3; SSH-3L; hSSH-3L
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MALVTVSRSPPGSGASTPVGPWDQAVQRRSRLQRRQSFAVLRGAVLGLQDGGDNDDAAEA
SSEPTEKAPSEEELHGDQTDFGQGSQSPQKQEEQRQHLHLMVQLLRPQDDIRLAAQLEAP
RPPRLRYLLVVSTREGEGLSQDETVLLGVDFPDSSSPSCTLGLVLPLWSDTQVYLDGDGG
FSVTSGGQSRIFKPISIQTMWATLQVLHQACEAALGSGLVPGGSALTWASHYQERLNSEQ
SCLNEWTAMADLESLRPPSAEPGGSSEQEQMEQAIRAELWKVLDVSDLESVTSKEIRQAL
ELRLGLPLQQYRDFIDNQMLLLVAQRDRASRIFPHLYLGSEWNAANLEELQRNRVTHILN
MAREIDNFYPERFTYHNVRLWDEESAQLLPHWKETHRFIEAARAQGTHVLVHCKMGVSRS
AATVLAYAMKQYECSLEQALRHVQELRPIARPNPGFLRQLQIYQGILTASRQSHVWEQKV
GGVSPEEHPAPEVSTPFPPLPPEPEGGGEEKVVGMEESQAAPKEEPGPRPRINLRGVMRS
ISLLEPSLELESTSETSDMPEVFSSHESSHEEPLQPFPQLARTKGGQQVDRGPQPALKSR
QSVVTLQGSAVVANRTQAFQEQEQGQGQGQGEPCISSTPRFRKVVRQASVHDSGEEGEA
Target Bioclass
Enzyme
Family
Protein-tyrosine phosphatase family
Subcellular location
Cytoplasm, cytoskeleton
Function
Protein phosphatase which may play a role in the regulation of actin filament dynamics. Can dephosphorylate and activate the actin binding/depolymerizing factor cofilin, which subsequently binds to actin filaments and stimulates their disassembly.
Uniprot ID
Q8TE77
Ensemble ID
ENST00000308127.9
HGNC ID
HGNC:30581

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
COLO800 SNV: p.Q24H DBIA    Probe Info 
DU145 SNV: p.M200T DBIA    Probe Info 
HCC15 Deletion: p.E348RfsTer12 .
JURKAT Deletion: p.P69RfsTer37 .
LNCaP clone FGC SNV: p.G339C DBIA    Probe Info 
MOLT4 SNV: p.R29M; p.Q298H; p.R372H .
NCIH358 SNV: p.S599P .
NUGC3 Deletion: p.E135del DBIA    Probe Info 
P31FUJ SNV: p.Q205H; p.E269V .
SG231 SNV: p.E486K DBIA    Probe Info 
SUPT1 SNV: p.W392L .
U937 SNV: p.L283M .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 5 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
Probe 1
 Probe Info  		Y311(21.30); Y369(25.62)  LDD3495  [1]
DBIA
 Probe Info  		C434(1.72)  LDD0532  [2]
IA-alkyne
 Probe Info  		N.A.  LDD0162  [3]
IPM
 Probe Info  		N.A.  LDD0025  [4]
NAIA_5
 Probe Info  		C413(0.00); C211(0.00); C434(0.00)  LDD2223  [5]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0215  AC10 HCT 116 C434(1.72)  LDD0532  [2]
 LDCM0226  AC11 HCT 116 C434(1.37)  LDD0543  [2]
 LDCM0237  AC12 HCT 116 C434(2.52)  LDD0554  [2]
 LDCM0259  AC14 HCT 116 C434(1.27)  LDD0576  [2]
 LDCM0263  AC143 PaTu 8988t C434(1.02)  LDD1142  [2]
 LDCM0264  AC144 PaTu 8988t C434(2.52)  LDD1143  [2]
 LDCM0265  AC145 PaTu 8988t C434(1.13)  LDD1144  [2]
 LDCM0266  AC146 PaTu 8988t C434(0.88)  LDD1145  [2]
 LDCM0267  AC147 PaTu 8988t C434(2.38)  LDD1146  [2]
 LDCM0268  AC148 PaTu 8988t C434(1.37)  LDD1147  [2]
 LDCM0269  AC149 PaTu 8988t C434(1.61)  LDD1148  [2]
 LDCM0270  AC15 HCT 116 C434(0.91)  LDD0587  [2]
 LDCM0271  AC150 PaTu 8988t C434(1.19)  LDD1150  [2]
 LDCM0272  AC151 PaTu 8988t C434(1.44)  LDD1151  [2]
 LDCM0273  AC152 PaTu 8988t C434(1.51)  LDD1152  [2]
 LDCM0274  AC153 PaTu 8988t C434(1.32)  LDD1153  [2]
 LDCM0621  AC154 PaTu 8988t C434(1.82)  LDD2166  [2]
 LDCM0622  AC155 PaTu 8988t C434(1.85)  LDD2167  [2]
 LDCM0623  AC156 PaTu 8988t C434(1.40)  LDD2168  [2]
 LDCM0624  AC157 PaTu 8988t C434(1.37)  LDD2169  [2]
 LDCM0323  AC6 HCT 116 C434(0.81)  LDD0640  [2]
 LDCM0334  AC7 HCT 116 C434(1.15)  LDD0651  [2]
 LDCM0345  AC8 HCT 116 C434(1.13)  LDD0662  [2]
 LDCM0248  AKOS034007472 HCT 116 C434(1.12)  LDD0565  [2]
 LDCM0356  AKOS034007680 HCT 116 C434(1.16)  LDD0673  [2]
 LDCM0275  AKOS034007705 HCT 116 C434(1.25)  LDD0592  [2]
 LDCM0632  CL-Sc Hep-G2 C413(20.00)  LDD2227  [5]
 LDCM0370  CL101 HCT 116 C434(1.05)  LDD0687  [2]
 LDCM0371  CL102 HCT 116 C434(1.52)  LDD0688  [2]
 LDCM0372  CL103 HCT 116 C434(1.03)  LDD0689  [2]
 LDCM0373  CL104 HCT 116 C434(1.42)  LDD0690  [2]
 LDCM0403  CL16 HCT 116 C434(1.00)  LDD0720  [2]
 LDCM0404  CL17 HCT 116 C434(0.91)  LDD0721  [2]
 LDCM0405  CL18 HCT 116 C434(0.60)  LDD0722  [2]
 LDCM0406  CL19 HCT 116 C434(0.65)  LDD0723  [2]
 LDCM0408  CL20 HCT 116 C434(0.72)  LDD0725  [2]
 LDCM0409  CL21 HCT 116 C434(0.73)  LDD0726  [2]
 LDCM0410  CL22 HCT 116 C434(0.79)  LDD0727  [2]
 LDCM0411  CL23 HCT 116 C434(0.76)  LDD0728  [2]
 LDCM0412  CL24 HCT 116 C434(0.95)  LDD0729  [2]
 LDCM0413  CL25 HCT 116 C434(0.68)  LDD0730  [2]
 LDCM0414  CL26 HCT 116 C434(0.58)  LDD0731  [2]
 LDCM0415  CL27 HCT 116 C434(0.73)  LDD0732  [2]
 LDCM0416  CL28 HCT 116 C434(0.68)  LDD0733  [2]
 LDCM0417  CL29 HCT 116 C434(0.97)  LDD0734  [2]
 LDCM0419  CL30 HCT 116 C434(0.96)  LDD0736  [2]
 LDCM0420  CL31 HCT 116 C434(0.98)  LDD0737  [2]
 LDCM0421  CL32 HCT 116 C434(1.45)  LDD0738  [2]
 LDCM0422  CL33 HCT 116 C434(1.02)  LDD0739  [2]
 LDCM0423  CL34 HCT 116 C434(1.36)  LDD0740  [2]
 LDCM0424  CL35 HCT 116 C434(0.73)  LDD0741  [2]
 LDCM0425  CL36 HCT 116 C434(1.18)  LDD0742  [2]
 LDCM0426  CL37 HCT 116 C434(1.09)  LDD0743  [2]
 LDCM0428  CL39 HCT 116 C434(1.08)  LDD0745  [2]
 LDCM0430  CL40 HCT 116 C434(1.41)  LDD0747  [2]
 LDCM0431  CL41 HCT 116 C434(1.32)  LDD0748  [2]
 LDCM0432  CL42 HCT 116 C434(1.49)  LDD0749  [2]
 LDCM0433  CL43 HCT 116 C434(1.31)  LDD0750  [2]
 LDCM0434  CL44 HCT 116 C434(1.10)  LDD0751  [2]
 LDCM0435  CL45 HCT 116 C434(1.07)  LDD0752  [2]
 LDCM0436  CL46 HCT 116 C434(1.07)  LDD0753  [2]
 LDCM0437  CL47 HCT 116 C434(1.07)  LDD0754  [2]
 LDCM0438  CL48 HCT 116 C434(1.17)  LDD0755  [2]
 LDCM0439  CL49 HCT 116 C434(1.37)  LDD0756  [2]
 LDCM0441  CL50 HCT 116 C434(1.11)  LDD0758  [2]
 LDCM0442  CL51 HCT 116 C434(1.16)  LDD0759  [2]
 LDCM0443  CL52 HCT 116 C434(1.10)  LDD0760  [2]
 LDCM0444  CL53 HCT 116 C434(1.27)  LDD0761  [2]
 LDCM0445  CL54 HCT 116 C434(0.86)  LDD0762  [2]
 LDCM0446  CL55 HCT 116 C434(1.12)  LDD0763  [2]
 LDCM0447  CL56 HCT 116 C434(1.11)  LDD0764  [2]
 LDCM0448  CL57 HCT 116 C434(1.06)  LDD0765  [2]
 LDCM0449  CL58 HCT 116 C434(1.07)  LDD0766  [2]
 LDCM0450  CL59 HCT 116 C434(1.36)  LDD0767  [2]
 LDCM0452  CL60 HCT 116 C434(1.48)  LDD0769  [2]
 LDCM0453  CL61 PaTu 8988t C434(0.97)  LDD1332  [2]
 LDCM0454  CL62 PaTu 8988t C434(2.81)  LDD1333  [2]
 LDCM0455  CL63 PaTu 8988t C434(1.63)  LDD1334  [2]
 LDCM0456  CL64 PaTu 8988t C434(1.32)  LDD1335  [2]
 LDCM0457  CL65 PaTu 8988t C434(1.19)  LDD1336  [2]
 LDCM0458  CL66 PaTu 8988t C434(2.18)  LDD1337  [2]
 LDCM0459  CL67 PaTu 8988t C434(1.45)  LDD1338  [2]
 LDCM0460  CL68 PaTu 8988t C434(1.20)  LDD1339  [2]
 LDCM0461  CL69 PaTu 8988t C434(1.39)  LDD1340  [2]
 LDCM0463  CL70 PaTu 8988t C434(1.38)  LDD1342  [2]
 LDCM0464  CL71 PaTu 8988t C434(1.16)  LDD1343  [2]
 LDCM0465  CL72 PaTu 8988t C434(1.41)  LDD1344  [2]
 LDCM0466  CL73 PaTu 8988t C434(1.12)  LDD1345  [2]
 LDCM0467  CL74 PaTu 8988t C434(1.02)  LDD1346  [2]
 LDCM0469  CL76 HCT 116 C434(0.61)  LDD0786  [2]
 LDCM0470  CL77 HCT 116 C434(0.73)  LDD0787  [2]
 LDCM0471  CL78 HCT 116 C434(0.41)  LDD0788  [2]
 LDCM0472  CL79 HCT 116 C434(0.81)  LDD0789  [2]
 LDCM0474  CL80 HCT 116 C434(0.59)  LDD0791  [2]
 LDCM0475  CL81 HCT 116 C434(0.56)  LDD0792  [2]
 LDCM0476  CL82 HCT 116 C434(0.56)  LDD0793  [2]
 LDCM0477  CL83 HCT 116 C434(0.56)  LDD0794  [2]
 LDCM0478  CL84 HCT 116 C434(0.76)  LDD0795  [2]
 LDCM0479  CL85 HCT 116 C434(0.54)  LDD0796  [2]
 LDCM0480  CL86 HCT 116 C434(0.64)  LDD0797  [2]
 LDCM0481  CL87 HCT 116 C434(0.77)  LDD0798  [2]
 LDCM0482  CL88 HCT 116 C434(0.56)  LDD0799  [2]
 LDCM0483  CL89 HCT 116 C434(0.83)  LDD0800  [2]
 LDCM0485  CL90 HCT 116 C434(0.70)  LDD0802  [2]
 LDCM0468  Fragment33 PaTu 8988t C434(1.25)  LDD1347  [2]
 LDCM0427  Fragment51 HCT 116 C434(1.00)  LDD0744  [2]
 LDCM0022  KB02 22RV1 C634(1.00)  LDD2243  [6]
 LDCM0023  KB03 22RV1 C634(0.87)  LDD2660  [6]
 LDCM0024  KB05 G361 C83(1.86)  LDD3311  [6]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Other
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Cytoplasmic protein NCK2 (NCK2) . O43639

References

1 An Azo Coupling-Based Chemoproteomic Approach to Systematically Profile the Tyrosine Reactivity in the Human Proteome. Anal Chem. 2021 Jul 27;93(29):10334-10342. doi: 10.1021/acs.analchem.1c01935. Epub 2021 Jul 12.
2 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
3 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
4 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
5 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
6 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840