Details of the Target

General Information of Target

Target ID LDTP09079
Target Name E3 ubiquitin-protein ligase RNF149 (RNF149)
Gene Name RNF149
Gene ID 284996
Synonyms
DNAPTP2; E3 ubiquitin-protein ligase RNF149; EC 2.3.2.27; DNA polymerase-transactivated protein 2; RING finger protein 149; RING-type E3 ubiquitin transferase RNF149
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MAWRRREASVGARGVLALALLALALCVPGARGRALEWFSAVVNIEYVDPQTNLTVWSVSE
SGRFGDSSPKEGAHGLVGVPWAPGGDLEGCAPDTRFFVPEPGGRGAAPWVALVARGGCTF
KDKVLVAARRNASAVVLYNEERYGNITLPMSHAGTGNIVVIMISYPKGREILELVQKGIP
VTMTIGVGTRHVQEFISGQSVVFVAIAFITMMIISLAWLIFYYIQRFLYTGSQIGSQSHR
KETKKVIGQLLLHTVKHGEKGIDVDAENCAVCIENFKVKDIIRILPCKHIFHRICIDPWL
LDHRTCPMCKLDVIKALGYWGEPGDVQEMPAPESPPGRDPAANLSLALPDDDGSDDSSPP
SASPAESEPQCDPSFKGDAGENTALLEAGRSDSRHGGPIS
Target Bioclass
Enzyme
Subcellular location
Membrane
Function E3 ubiquitin-protein ligase. Ubiquitinates BRAF, inducing its proteasomal degradation.
Uniprot ID
Q8NC42
Ensemble ID
ENST00000295317.4
HGNC ID
HGNC:23137

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 6 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
JZ128-DTB
 Probe Info  		N.A.  LDD0462  [1]
DBIA
 Probe Info  		C295(0.91)  LDD0531  [2]
4-Iodoacetamidophenylacetylene
 Probe Info  		N.A.  LDD0038  [3]
IA-alkyne
 Probe Info  		N.A.  LDD0036  [3]
NAIA_4
 Probe Info  		N.A.  LDD2226  [4]
NAIA_5
 Probe Info  		N.A.  LDD2224  [4]
PAL-AfBPP Probe
Click To Hide/Show 1 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C170
 Probe Info  		14.42  LDD1850  [5]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0214  AC1 HCT 116 C295(0.91)  LDD0531  [2]
 LDCM0215  AC10 HCT 116 C295(1.09)  LDD0532  [2]
 LDCM0226  AC11 HCT 116 C295(0.96)  LDD0543  [2]
 LDCM0237  AC12 HCT 116 C295(0.89)  LDD0554  [2]
 LDCM0259  AC14 HCT 116 C295(1.18)  LDD0576  [2]
 LDCM0270  AC15 HCT 116 C295(1.03)  LDD0587  [2]
 LDCM0276  AC17 HCT 116 C269(0.83); C272(0.83); C295(0.97)  LDD0593  [2]
 LDCM0277  AC18 HCT 116 C269(1.02); C272(1.02); C295(1.22)  LDD0594  [2]
 LDCM0278  AC19 HCT 116 C269(0.97); C272(0.97); C295(1.00)  LDD0595  [2]
 LDCM0279  AC2 HCT 116 C295(0.94)  LDD0596  [2]
 LDCM0280  AC20 HCT 116 C269(0.88); C272(0.88); C295(1.11)  LDD0597  [2]
 LDCM0281  AC21 HCT 116 C269(0.95); C272(0.95); C295(0.96)  LDD0598  [2]
 LDCM0282  AC22 HCT 116 C269(0.84); C272(0.84); C295(0.91)  LDD0599  [2]
 LDCM0283  AC23 HCT 116 C269(0.81); C272(0.81); C295(0.91)  LDD0600  [2]
 LDCM0284  AC24 HCT 116 C269(0.86); C272(0.86); C295(0.99)  LDD0601  [2]
 LDCM0285  AC25 HCT 116 C295(1.01); C269(1.07); C272(1.07)  LDD0602  [2]
 LDCM0286  AC26 HCT 116 C269(1.15); C272(1.15); C295(1.16)  LDD0603  [2]
 LDCM0287  AC27 HCT 116 C295(1.13); C269(1.47); C272(1.47)  LDD0604  [2]
 LDCM0288  AC28 HCT 116 C295(1.12); C269(1.40); C272(1.40)  LDD0605  [2]
 LDCM0289  AC29 HCT 116 C295(1.23); C269(1.30); C272(1.30)  LDD0606  [2]
 LDCM0290  AC3 HCT 116 C295(0.99)  LDD0607  [2]
 LDCM0291  AC30 HCT 116 C295(1.21); C269(1.38); C272(1.38)  LDD0608  [2]
 LDCM0292  AC31 HCT 116 C295(1.18); C269(1.33); C272(1.33)  LDD0609  [2]
 LDCM0293  AC32 HCT 116 C269(1.39); C272(1.39); C295(1.41)  LDD0610  [2]
 LDCM0294  AC33 HCT 116 C295(1.30); C269(1.79); C272(1.79)  LDD0611  [2]
 LDCM0295  AC34 HCT 116 C295(1.27); C269(1.42); C272(1.42)  LDD0612  [2]
 LDCM0296  AC35 HCT 116 C295(0.97)  LDD0613  [2]
 LDCM0297  AC36 HCT 116 C295(1.04)  LDD0614  [2]
 LDCM0298  AC37 HCT 116 C295(0.98)  LDD0615  [2]
 LDCM0299  AC38 HCT 116 C295(0.96)  LDD0616  [2]
 LDCM0300  AC39 HCT 116 C295(1.22)  LDD0617  [2]
 LDCM0301  AC4 HCT 116 C295(1.06)  LDD0618  [2]
 LDCM0302  AC40 HCT 116 C295(1.15)  LDD0619  [2]
 LDCM0303  AC41 HCT 116 C295(1.03)  LDD0620  [2]
 LDCM0304  AC42 HCT 116 C295(1.12)  LDD0621  [2]
 LDCM0305  AC43 HCT 116 C295(1.26)  LDD0622  [2]
 LDCM0306  AC44 HCT 116 C295(1.24)  LDD0623  [2]
 LDCM0307  AC45 HCT 116 C295(1.14)  LDD0624  [2]
 LDCM0308  AC46 HEK-293T C295(0.91); C269(1.22)  LDD1547  [6]
 LDCM0309  AC47 HEK-293T C295(1.00)  LDD1548  [6]
 LDCM0310  AC48 HEK-293T C269(1.03)  LDD1549  [6]
 LDCM0312  AC5 HCT 116 C295(1.15)  LDD0629  [2]
 LDCM0313  AC50 HEK-293T C295(0.96)  LDD1552  [6]
 LDCM0315  AC52 HEK-293T C295(0.96)  LDD1554  [6]
 LDCM0316  AC53 HEK-293T C295(1.01); C90(0.92); C269(1.00)  LDD1555  [6]
 LDCM0317  AC54 HEK-293T C295(0.98); C269(1.07)  LDD1556  [6]
 LDCM0318  AC55 HEK-293T C295(1.00)  LDD1557  [6]
 LDCM0319  AC56 HEK-293T C269(1.07)  LDD1558  [6]
 LDCM0320  AC57 HCT 116 C269(1.06); C272(1.06); C295(1.43)  LDD0637  [2]
 LDCM0321  AC58 HCT 116 C295(1.14); C269(1.18); C272(1.18)  LDD0638  [2]
 LDCM0322  AC59 HCT 116 C269(1.28); C272(1.28); C295(1.47)  LDD0639  [2]
 LDCM0323  AC6 HCT 116 C295(1.15)  LDD0640  [2]
 LDCM0324  AC60 HCT 116 C269(1.01); C272(1.01); C295(1.28)  LDD0641  [2]
 LDCM0325  AC61 HCT 116 C269(0.98); C272(0.98); C295(1.12)  LDD0642  [2]
 LDCM0326  AC62 HCT 116 C269(1.12); C272(1.12); C295(1.35)  LDD0643  [2]
 LDCM0327  AC63 HCT 116 C269(1.00); C272(1.00); C295(1.05)  LDD0644  [2]
 LDCM0328  AC64 HCT 116 C269(1.22); C272(1.22); C295(1.39)  LDD0645  [2]
 LDCM0329  AC65 HCT 116 C269(1.83); C272(1.83); C295(2.45)  LDD0646  [2]
 LDCM0330  AC66 HCT 116 C269(1.76); C272(1.76); C295(2.40)  LDD0647  [2]
 LDCM0331  AC67 HCT 116 C269(1.49); C272(1.49); C295(1.92)  LDD0648  [2]
 LDCM0334  AC7 HCT 116 C295(1.11)  LDD0651  [2]
 LDCM0345  AC8 HCT 116 C295(1.04)  LDD0662  [2]
 LDCM0248  AKOS034007472 HCT 116 C295(1.00)  LDD0565  [2]
 LDCM0356  AKOS034007680 HCT 116 C295(1.11)  LDD0673  [2]
 LDCM0275  AKOS034007705 HCT 116 C295(1.22)  LDD0592  [2]
 LDCM0367  CL1 HCT 116 C269(0.73); C272(0.73); C295(0.94)  LDD0684  [2]
 LDCM0368  CL10 HCT 116 C269(0.81); C272(0.81); C295(1.09)  LDD0685  [2]
 LDCM0369  CL100 HCT 116 C295(0.88)  LDD0686  [2]
 LDCM0370  CL101 HCT 116 C295(1.04)  LDD0687  [2]
 LDCM0371  CL102 HCT 116 C295(1.18)  LDD0688  [2]
 LDCM0372  CL103 HCT 116 C295(1.03)  LDD0689  [2]
 LDCM0373  CL104 HCT 116 C295(0.93)  LDD0690  [2]
 LDCM0374  CL105 HCT 116 C269(0.94); C272(0.94); C295(1.09)  LDD0691  [2]
 LDCM0375  CL106 HCT 116 C269(0.97); C272(0.97); C295(1.23)  LDD0692  [2]
 LDCM0376  CL107 HCT 116 C269(0.96); C272(0.96); C295(1.17)  LDD0693  [2]
 LDCM0377  CL108 HCT 116 C269(1.00); C272(1.00); C295(1.24)  LDD0694  [2]
 LDCM0378  CL109 HCT 116 C269(0.98); C272(0.98); C295(1.12)  LDD0695  [2]
 LDCM0379  CL11 HCT 116 C269(0.98); C272(0.98); C295(1.12)  LDD0696  [2]
 LDCM0380  CL110 HCT 116 C269(0.95); C272(0.95); C295(1.14)  LDD0697  [2]
 LDCM0381  CL111 HCT 116 C269(0.86); C272(0.86); C295(1.08)  LDD0698  [2]
 LDCM0382  CL112 HCT 116 C295(1.00); C269(1.36); C272(1.36)  LDD0699  [2]
 LDCM0383  CL113 HCT 116 C295(1.26); C269(1.36); C272(1.36)  LDD0700  [2]
 LDCM0384  CL114 HCT 116 C295(1.05); C269(1.38); C272(1.38)  LDD0701  [2]
 LDCM0385  CL115 HCT 116 C295(1.19); C269(1.26); C272(1.26)  LDD0702  [2]
 LDCM0386  CL116 HCT 116 C295(1.41); C269(1.57); C272(1.57)  LDD0703  [2]
 LDCM0387  CL117 HCT 116 C295(1.37)  LDD0704  [2]
 LDCM0388  CL118 HCT 116 C295(1.19)  LDD0705  [2]
 LDCM0389  CL119 HCT 116 C295(1.01)  LDD0706  [2]
 LDCM0390  CL12 HCT 116 C269(0.90); C272(0.90); C295(1.31)  LDD0707  [2]
 LDCM0391  CL120 HCT 116 C295(1.12)  LDD0708  [2]
 LDCM0392  CL121 HEK-293T C295(0.93)  LDD1596  [6]
 LDCM0393  CL122 HEK-293T C295(1.05)  LDD1597  [6]
 LDCM0396  CL125 HCT 116 C269(0.92); C272(0.92); C295(1.07)  LDD0713  [2]
 LDCM0397  CL126 HCT 116 C295(1.05); C269(1.06); C272(1.06)  LDD0714  [2]
 LDCM0398  CL127 HCT 116 C295(0.84); C269(0.91); C272(0.91)  LDD0715  [2]
 LDCM0399  CL128 HCT 116 C269(1.25); C272(1.25); C295(1.46)  LDD0716  [2]
 LDCM0400  CL13 HCT 116 C269(0.90); C272(0.90); C295(1.05)  LDD0717  [2]
 LDCM0401  CL14 HCT 116 C269(1.01); C272(1.01); C295(1.08)  LDD0718  [2]
 LDCM0402  CL15 HCT 116 C295(1.08); C269(1.13); C272(1.13)  LDD0719  [2]
 LDCM0403  CL16 HCT 116 C295(0.84)  LDD0720  [2]
 LDCM0404  CL17 HCT 116 C295(0.92)  LDD0721  [2]
 LDCM0405  CL18 HCT 116 C295(1.06)  LDD0722  [2]
 LDCM0406  CL19 HCT 116 C295(1.00)  LDD0723  [2]
 LDCM0407  CL2 HCT 116 C269(0.73); C272(0.73); C295(0.83)  LDD0724  [2]
 LDCM0408  CL20 HCT 116 C295(1.07)  LDD0725  [2]
 LDCM0409  CL21 HCT 116 C295(1.10)  LDD0726  [2]
 LDCM0410  CL22 HCT 116 C295(1.31)  LDD0727  [2]
 LDCM0411  CL23 HCT 116 C295(1.07)  LDD0728  [2]
 LDCM0412  CL24 HCT 116 C295(1.09)  LDD0729  [2]
 LDCM0413  CL25 HCT 116 C295(0.89)  LDD0730  [2]
 LDCM0414  CL26 HCT 116 C295(0.92)  LDD0731  [2]
 LDCM0415  CL27 HCT 116 C295(1.05)  LDD0732  [2]
 LDCM0416  CL28 HCT 116 C295(1.04)  LDD0733  [2]
 LDCM0417  CL29 HCT 116 C295(1.11)  LDD0734  [2]
 LDCM0418  CL3 HCT 116 C269(0.94); C272(0.94); C295(1.05)  LDD0735  [2]
 LDCM0419  CL30 HCT 116 C295(0.98)  LDD0736  [2]
 LDCM0421  CL32 HEK-293T C295(1.05)  LDD1625  [6]
 LDCM0422  CL33 HEK-293T C295(1.00); C90(2.34); C269(1.14)  LDD1626  [6]
 LDCM0423  CL34 HEK-293T C295(0.90); C269(1.11)  LDD1627  [6]
 LDCM0424  CL35 HEK-293T C295(0.97)  LDD1628  [6]
 LDCM0425  CL36 HEK-293T C269(1.13)  LDD1629  [6]
 LDCM0426  CL37 HEK-293T C295(1.01)  LDD1630  [6]
 LDCM0429  CL4 HCT 116 C269(0.97); C272(0.97); C295(0.90)  LDD0746  [2]
 LDCM0432  CL42 HEK-293T C295(0.92)  LDD1636  [6]
 LDCM0434  CL44 HEK-293T C295(1.09)  LDD1638  [6]
 LDCM0435  CL45 HEK-293T C295(1.03); C90(4.34); C269(1.11)  LDD1639  [6]
 LDCM0436  CL46 HEK-293T C295(0.95); C269(1.23)  LDD1640  [6]
 LDCM0437  CL47 HEK-293T C295(0.91)  LDD1641  [6]
 LDCM0438  CL48 HEK-293T C269(1.18)  LDD1642  [6]
 LDCM0439  CL49 HEK-293T C295(1.09)  LDD1643  [6]
 LDCM0440  CL5 HCT 116 C269(0.85); C272(0.85); C295(0.99)  LDD0757  [2]
 LDCM0441  CL50 HEK-293T C295(1.00)  LDD1645  [6]
 LDCM0445  CL54 HEK-293T C295(1.01)  LDD1648  [6]
 LDCM0447  CL56 HEK-293T C295(1.09)  LDD1650  [6]
 LDCM0448  CL57 HEK-293T C295(1.06); C90(3.69); C269(0.98)  LDD1651  [6]
 LDCM0449  CL58 HEK-293T C295(0.91); C269(1.13)  LDD1652  [6]
 LDCM0450  CL59 HEK-293T C295(0.99)  LDD1653  [6]
 LDCM0451  CL6 HCT 116 C269(0.88); C272(0.88); C295(0.89)  LDD0768  [2]
 LDCM0452  CL60 HEK-293T C269(1.17)  LDD1655  [6]
 LDCM0453  CL61 HCT 116 C295(1.08)  LDD0770  [2]
 LDCM0454  CL62 HCT 116 C295(0.92)  LDD0771  [2]
 LDCM0455  CL63 HCT 116 C295(1.16)  LDD0772  [2]
 LDCM0456  CL64 HCT 116 C295(1.09)  LDD0773  [2]
 LDCM0457  CL65 HCT 116 C295(1.09)  LDD0774  [2]
 LDCM0458  CL66 HCT 116 C295(1.26)  LDD0775  [2]
 LDCM0459  CL67 HCT 116 C295(1.18)  LDD0776  [2]
 LDCM0460  CL68 HCT 116 C295(1.20)  LDD0777  [2]
 LDCM0461  CL69 HCT 116 C295(0.96)  LDD0778  [2]
 LDCM0462  CL7 HCT 116 C269(0.94); C272(0.94); C295(1.32)  LDD0779  [2]
 LDCM0463  CL70 HCT 116 C295(1.10)  LDD0780  [2]
 LDCM0464  CL71 HCT 116 C295(1.03)  LDD0781  [2]
 LDCM0465  CL72 HCT 116 C295(1.06)  LDD0782  [2]
 LDCM0466  CL73 HCT 116 C295(1.13)  LDD0783  [2]
 LDCM0467  CL74 HCT 116 C295(1.12)  LDD0784  [2]
 LDCM0471  CL78 HEK-293T C295(1.05)  LDD1674  [6]
 LDCM0473  CL8 HCT 116 C269(1.06); C272(1.06); C295(1.32)  LDD0790  [2]
 LDCM0474  CL80 HEK-293T C295(1.07)  LDD1677  [6]
 LDCM0475  CL81 HEK-293T C295(1.05); C90(0.63); C269(1.03)  LDD1678  [6]
 LDCM0476  CL82 HEK-293T C295(0.94); C269(1.30)  LDD1679  [6]
 LDCM0477  CL83 HEK-293T C295(0.98)  LDD1680  [6]
 LDCM0478  CL84 HEK-293T C269(1.32)  LDD1681  [6]
 LDCM0479  CL85 HEK-293T C295(1.07)  LDD1682  [6]
 LDCM0480  CL86 HEK-293T C295(1.12)  LDD1683  [6]
 LDCM0484  CL9 HCT 116 C269(0.74); C272(0.74); C295(0.93)  LDD0801  [2]
 LDCM0485  CL90 HEK-293T C295(1.11)  LDD1688  [6]
 LDCM0486  CL91 HCT 116 C295(1.26)  LDD0803  [2]
 LDCM0487  CL92 HCT 116 C295(1.02)  LDD0804  [2]
 LDCM0488  CL93 HCT 116 C295(1.25)  LDD0805  [2]
 LDCM0489  CL94 HCT 116 C295(1.54)  LDD0806  [2]
 LDCM0490  CL95 HCT 116 C295(1.24)  LDD0807  [2]
 LDCM0491  CL96 HCT 116 C295(1.16)  LDD0808  [2]
 LDCM0492  CL97 HCT 116 C295(0.94)  LDD0809  [2]
 LDCM0493  CL98 HCT 116 C295(0.89)  LDD0810  [2]
 LDCM0494  CL99 HCT 116 C295(0.89)  LDD0811  [2]
 LDCM0468  Fragment33 HCT 116 C295(1.21)  LDD0785  [2]
 LDCM0427  Fragment51 HEK-293T C295(1.08)  LDD1631  [6]
 LDCM0179  JZ128 PC-3 N.A.  LDD0462  [1]
 LDCM0022  KB02 HEK-293T C295(1.03)  LDD1492  [6]
 LDCM0023  KB03 HEK-293T C295(1.02)  LDD1497  [6]
 LDCM0024  KB05 COLO792 C295(1.76)  LDD3310  [7]

References

1 A Chemoproteomic Strategy for Direct and Proteome-Wide Covalent Inhibitor Target-Site Identification. J Am Chem Soc. 2019 Jan 9;141(1):191-203. doi: 10.1021/jacs.8b07911. Epub 2018 Dec 20.
2 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
3 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
4 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
5 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587
6 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
7 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840