Details of the Target

General Information of Target

Target ID LDTP06887
Target Name Nuclear cap-binding protein subunit 3 (NCBP3)
Gene Name NCBP3
Gene ID 55421
Synonyms
C17orf85; Nuclear cap-binding protein subunit 3; Protein ELG
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MAAVRGLRVSVKAEAPAGPALGLPSPEAESGVDRGEPEPMEVEEGELEIVPVRRSLKELI
PDTSRRYENKAGSFITGIDVTSKEAIEKKEQRAKRFHFRSEVNLAQRNVALDRDMMKKAI
PKVRLETIYICGVDEMSTQDVFSYFKEYPPAHIEWLDDTSCNVVWLDEMTATRALINMSS
LPAQDKIRSRDASEDKSAEKRKKDKQEDSSDDDEAEEGEVEDENSSDVELDTLSQVEEES
LLRNDLRPANKLAKGNRLFMRFATKDDKKELGAARRSQYYMKYGNPNYGGMKGILSNSWK
RRYHSRRIQRDVIKKRALIGDDVGLTSYKHRHSGLVNVPEEPIEEEEEEEEEEEEEEEED
QDMDADDRVVVEYHEELPALKQPRERSASRRSSASSSDSDEMDYDLELKMISTPSPKKSM
KMTMYADEVESQLKNIRNSMRADSVSSSNIKNRIGNKLPPEKFADVRHLLDEKRQHSRPR
PPVSSTKSDIRQRLGKRPHSPEKAFSSNPVVRREPSSDVHSRLGVPRQDSKGLYADTREK
KSGNLWTRLGSAPKTKEKNTKKVDHRAPGAEEDDSELQRAWGALIKEKEQSRQKKSRLDN
LPSLQIEVSRESSSGSEAES
Target Bioclass
Other
Family
NCBP3 family
Subcellular location
Nucleus
Function
Associates with NCBP1/CBP80 to form an alternative cap-binding complex (CBC) which plays a key role in mRNA export. NCBP3 serves as adapter protein linking the capped RNAs (m7GpppG-capped RNA) to NCBP1/CBP80. Unlike the conventional CBC with NCBP2 which binds both small nuclear RNA (snRNA) and messenger (mRNA) and is involved in their export from the nucleus, the alternative CBC with NCBP3 does not bind snRNA and associates only with mRNA thereby playing a role in only mRNA export. The alternative CBC is particularly important in cellular stress situations such as virus infections and the NCBP3 activity is critical to inhibit virus growth.
Uniprot ID
Q53F19
Ensemble ID
ENST00000389005.6
HGNC ID
HGNC:24612

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
CMK SNV: p.A426G .
MDST8 SNV: p.H304Y .
MEWO SNV: p.S143F .
MOLT4 SNV: p.A20T; p.S444G .
OVK18 SNV: p.I313N .
RKO SNV: p.R453Ter .
RPMI8226 SNV: p.K314N .
SW1271 SNV: p.G18W .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 7 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
STPyne
 Probe Info  		K329(2.78); K496(1.00)  LDD0277  [1]
Probe 1
 Probe Info  		Y283(15.54)  LDD3495  [2]
DBIA
 Probe Info  		C131(0.96)  LDD1507  [3]
m-APA
 Probe Info  		N.A.  LDD2231  [4]
4-Iodoacetamidophenylacetylene
 Probe Info  		N.A.  LDD0038  [5]
Lodoacetamide azide
 Probe Info  		N.A.  LDD0037  [5]
SF
 Probe Info  		N.A.  LDD0028  [6]
PAL-AfBPP Probe
Click To Hide/Show 3 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C389
 Probe Info  		5.78  LDD2048  [7]
C420
 Probe Info  		14.93  LDD2075  [7]
C430
 Probe Info  		5.03  LDD2085  [7]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0214  AC1 HEK-293T C131(0.96)  LDD1507  [3]
 LDCM0215  AC10 HEK-293T C131(0.98)  LDD1508  [3]
 LDCM0226  AC11 HEK-293T C131(1.08)  LDD1509  [3]
 LDCM0237  AC12 HEK-293T C131(1.15)  LDD1510  [3]
 LDCM0259  AC14 HEK-293T C131(0.98)  LDD1512  [3]
 LDCM0270  AC15 HEK-293T C131(1.10)  LDD1513  [3]
 LDCM0276  AC17 HEK-293T C131(0.85)  LDD1515  [3]
 LDCM0277  AC18 HEK-293T C131(0.86)  LDD1516  [3]
 LDCM0278  AC19 HEK-293T C131(0.90)  LDD1517  [3]
 LDCM0279  AC2 HEK-293T C131(1.05)  LDD1518  [3]
 LDCM0280  AC20 HEK-293T C131(0.95)  LDD1519  [3]
 LDCM0281  AC21 HEK-293T C131(1.03)  LDD1520  [3]
 LDCM0282  AC22 HEK-293T C131(0.90)  LDD1521  [3]
 LDCM0283  AC23 HEK-293T C131(1.37)  LDD1522  [3]
 LDCM0284  AC24 HEK-293T C131(0.87)  LDD1523  [3]
 LDCM0285  AC25 HEK-293T C131(0.89)  LDD1524  [3]
 LDCM0286  AC26 HEK-293T C131(1.04)  LDD1525  [3]
 LDCM0287  AC27 HEK-293T C131(1.27)  LDD1526  [3]
 LDCM0288  AC28 HEK-293T C131(0.97)  LDD1527  [3]
 LDCM0289  AC29 HEK-293T C131(0.97)  LDD1528  [3]
 LDCM0290  AC3 HEK-293T C131(0.99)  LDD1529  [3]
 LDCM0291  AC30 HEK-293T C131(1.04)  LDD1530  [3]
 LDCM0292  AC31 HEK-293T C131(1.39)  LDD1531  [3]
 LDCM0293  AC32 HEK-293T C131(1.00)  LDD1532  [3]
 LDCM0294  AC33 HEK-293T C131(1.06)  LDD1533  [3]
 LDCM0295  AC34 HEK-293T C131(0.99)  LDD1534  [3]
 LDCM0296  AC35 HEK-293T C131(1.07)  LDD1535  [3]
 LDCM0297  AC36 HEK-293T C131(1.12)  LDD1536  [3]
 LDCM0298  AC37 HEK-293T C131(1.00)  LDD1537  [3]
 LDCM0299  AC38 HEK-293T C131(1.09)  LDD1538  [3]
 LDCM0300  AC39 HEK-293T C131(1.39)  LDD1539  [3]
 LDCM0301  AC4 HEK-293T C131(1.14)  LDD1540  [3]
 LDCM0302  AC40 HEK-293T C131(0.98)  LDD1541  [3]
 LDCM0303  AC41 HEK-293T C131(0.90)  LDD1542  [3]
 LDCM0304  AC42 HEK-293T C131(0.94)  LDD1543  [3]
 LDCM0305  AC43 HEK-293T C131(1.02)  LDD1544  [3]
 LDCM0306  AC44 HEK-293T C131(1.25)  LDD1545  [3]
 LDCM0307  AC45 HEK-293T C131(1.03)  LDD1546  [3]
 LDCM0308  AC46 HEK-293T C131(1.19)  LDD1547  [3]
 LDCM0309  AC47 HEK-293T C131(1.30)  LDD1548  [3]
 LDCM0310  AC48 HEK-293T C131(1.06)  LDD1549  [3]
 LDCM0311  AC49 HEK-293T C131(0.82)  LDD1550  [3]
 LDCM0312  AC5 HEK-293T C131(0.94)  LDD1551  [3]
 LDCM0313  AC50 HEK-293T C131(1.05)  LDD1552  [3]
 LDCM0314  AC51 HEK-293T C131(0.92)  LDD1553  [3]
 LDCM0315  AC52 HEK-293T C131(1.26)  LDD1554  [3]
 LDCM0316  AC53 HEK-293T C131(1.11)  LDD1555  [3]
 LDCM0317  AC54 HEK-293T C131(0.95)  LDD1556  [3]
 LDCM0318  AC55 HEK-293T C131(1.31)  LDD1557  [3]
 LDCM0319  AC56 HEK-293T C131(0.88)  LDD1558  [3]
 LDCM0320  AC57 HEK-293T C131(1.05)  LDD1559  [3]
 LDCM0321  AC58 HEK-293T C131(0.95)  LDD1560  [3]
 LDCM0322  AC59 HEK-293T C131(1.03)  LDD1561  [3]
 LDCM0323  AC6 HEK-293T C131(1.00)  LDD1562  [3]
 LDCM0324  AC60 HEK-293T C131(1.05)  LDD1563  [3]
 LDCM0325  AC61 HEK-293T C131(1.06)  LDD1564  [3]
 LDCM0326  AC62 HEK-293T C131(1.00)  LDD1565  [3]
 LDCM0327  AC63 HEK-293T C131(1.11)  LDD1566  [3]
 LDCM0328  AC64 HEK-293T C131(1.07)  LDD1567  [3]
 LDCM0334  AC7 HEK-293T C131(1.15)  LDD1568  [3]
 LDCM0345  AC8 HEK-293T C131(1.01)  LDD1569  [3]
 LDCM0248  AKOS034007472 HEK-293T C131(1.04)  LDD1511  [3]
 LDCM0356  AKOS034007680 HEK-293T C131(1.01)  LDD1570  [3]
 LDCM0275  AKOS034007705 HEK-293T C131(1.02)  LDD1514  [3]
 LDCM0367  CL1 HEK-293T C131(1.22)  LDD1571  [3]
 LDCM0368  CL10 HEK-293T C131(0.93)  LDD1572  [3]
 LDCM0369  CL100 HEK-293T C131(1.08)  LDD1573  [3]
 LDCM0370  CL101 HEK-293T C131(1.07)  LDD1574  [3]
 LDCM0371  CL102 HEK-293T C131(0.76)  LDD1575  [3]
 LDCM0372  CL103 HEK-293T C131(1.10)  LDD1576  [3]
 LDCM0373  CL104 HEK-293T C131(1.06)  LDD1577  [3]
 LDCM0374  CL105 HEK-293T C131(1.11)  LDD1578  [3]
 LDCM0375  CL106 HEK-293T C131(0.92)  LDD1579  [3]
 LDCM0376  CL107 HEK-293T C131(0.91)  LDD1580  [3]
 LDCM0377  CL108 HEK-293T C131(1.02)  LDD1581  [3]
 LDCM0378  CL109 HEK-293T C131(1.04)  LDD1582  [3]
 LDCM0379  CL11 HEK-293T C131(0.95)  LDD1583  [3]
 LDCM0380  CL110 HEK-293T C131(1.00)  LDD1584  [3]
 LDCM0381  CL111 HEK-293T C131(1.00)  LDD1585  [3]
 LDCM0382  CL112 HEK-293T C131(0.89)  LDD1586  [3]
 LDCM0383  CL113 HEK-293T C131(1.27)  LDD1587  [3]
 LDCM0384  CL114 HEK-293T C131(1.06)  LDD1588  [3]
 LDCM0385  CL115 HEK-293T C131(1.10)  LDD1589  [3]
 LDCM0386  CL116 HEK-293T C131(1.07)  LDD1590  [3]
 LDCM0387  CL117 HEK-293T C131(1.03)  LDD1591  [3]
 LDCM0388  CL118 HEK-293T C131(0.97)  LDD1592  [3]
 LDCM0389  CL119 HEK-293T C131(1.09)  LDD1593  [3]
 LDCM0390  CL12 HEK-293T C131(1.02)  LDD1594  [3]
 LDCM0391  CL120 HEK-293T C131(1.28)  LDD1595  [3]
 LDCM0392  CL121 HEK-293T C131(0.93)  LDD1596  [3]
 LDCM0393  CL122 HEK-293T C131(1.13)  LDD1597  [3]
 LDCM0394  CL123 HEK-293T C131(0.87)  LDD1598  [3]
 LDCM0395  CL124 HEK-293T C131(1.12)  LDD1599  [3]
 LDCM0396  CL125 HEK-293T C131(0.98)  LDD1600  [3]
 LDCM0397  CL126 HEK-293T C131(1.19)  LDD1601  [3]
 LDCM0398  CL127 HEK-293T C131(0.88)  LDD1602  [3]
 LDCM0399  CL128 HEK-293T C131(1.14)  LDD1603  [3]
 LDCM0400  CL13 HEK-293T C131(1.01)  LDD1604  [3]
 LDCM0401  CL14 HEK-293T C131(1.00)  LDD1605  [3]
 LDCM0402  CL15 HEK-293T C131(0.90)  LDD1606  [3]
 LDCM0403  CL16 HEK-293T C131(1.26)  LDD1607  [3]
 LDCM0404  CL17 HEK-293T C131(0.91)  LDD1608  [3]
 LDCM0405  CL18 HEK-293T C131(1.09)  LDD1609  [3]
 LDCM0406  CL19 HEK-293T C131(1.22)  LDD1610  [3]
 LDCM0407  CL2 HEK-293T C131(1.19)  LDD1611  [3]
 LDCM0408  CL20 HEK-293T C131(1.12)  LDD1612  [3]
 LDCM0409  CL21 HEK-293T C131(0.82)  LDD1613  [3]
 LDCM0410  CL22 HEK-293T C131(1.00)  LDD1614  [3]
 LDCM0411  CL23 HEK-293T C131(1.42)  LDD1615  [3]
 LDCM0412  CL24 HEK-293T C131(1.00)  LDD1616  [3]
 LDCM0413  CL25 HEK-293T C131(1.12)  LDD1617  [3]
 LDCM0414  CL26 HEK-293T C131(0.99)  LDD1618  [3]
 LDCM0415  CL27 HEK-293T C131(0.98)  LDD1619  [3]
 LDCM0416  CL28 HEK-293T C131(0.83)  LDD1620  [3]
 LDCM0417  CL29 HEK-293T C131(0.97)  LDD1621  [3]
 LDCM0418  CL3 HEK-293T C131(1.11)  LDD1622  [3]
 LDCM0419  CL30 HEK-293T C131(0.97)  LDD1623  [3]
 LDCM0420  CL31 HEK-293T C131(0.94)  LDD1624  [3]
 LDCM0421  CL32 HEK-293T C131(0.95)  LDD1625  [3]
 LDCM0422  CL33 HEK-293T C131(0.85)  LDD1626  [3]
 LDCM0423  CL34 HEK-293T C131(0.99)  LDD1627  [3]
 LDCM0424  CL35 HEK-293T C131(1.21)  LDD1628  [3]
 LDCM0425  CL36 HEK-293T C131(1.06)  LDD1629  [3]
 LDCM0426  CL37 HEK-293T C131(1.15)  LDD1630  [3]
 LDCM0428  CL39 HEK-293T C131(1.01)  LDD1632  [3]
 LDCM0429  CL4 HEK-293T C131(1.08)  LDD1633  [3]
 LDCM0430  CL40 HEK-293T C131(0.99)  LDD1634  [3]
 LDCM0431  CL41 HEK-293T C131(0.87)  LDD1635  [3]
 LDCM0432  CL42 HEK-293T C131(1.14)  LDD1636  [3]
 LDCM0433  CL43 HEK-293T C131(1.15)  LDD1637  [3]
 LDCM0434  CL44 HEK-293T C131(1.06)  LDD1638  [3]
 LDCM0435  CL45 HEK-293T C131(0.99)  LDD1639  [3]
 LDCM0436  CL46 HEK-293T C131(0.75)  LDD1640  [3]
 LDCM0437  CL47 HEK-293T C131(1.28)  LDD1641  [3]
 LDCM0438  CL48 HEK-293T C131(0.92)  LDD1642  [3]
 LDCM0439  CL49 HEK-293T C131(1.19)  LDD1643  [3]
 LDCM0440  CL5 HEK-293T C131(1.03)  LDD1644  [3]
 LDCM0441  CL50 HEK-293T C131(1.14)  LDD1645  [3]
 LDCM0443  CL52 HEK-293T C131(1.16)  LDD1646  [3]
 LDCM0444  CL53 HEK-293T C131(0.90)  LDD1647  [3]
 LDCM0445  CL54 HEK-293T C131(0.90)  LDD1648  [3]
 LDCM0446  CL55 HEK-293T C131(1.21)  LDD1649  [3]
 LDCM0447  CL56 HEK-293T C131(1.09)  LDD1650  [3]
 LDCM0448  CL57 HEK-293T C131(1.00)  LDD1651  [3]
 LDCM0449  CL58 HEK-293T C131(1.04)  LDD1652  [3]
 LDCM0450  CL59 HEK-293T C131(1.26)  LDD1653  [3]
 LDCM0451  CL6 HEK-293T C131(0.97)  LDD1654  [3]
 LDCM0452  CL60 HEK-293T C131(1.08)  LDD1655  [3]
 LDCM0453  CL61 HEK-293T C131(1.20)  LDD1656  [3]
 LDCM0454  CL62 HEK-293T C131(1.03)  LDD1657  [3]
 LDCM0455  CL63 HEK-293T C131(0.98)  LDD1658  [3]
 LDCM0456  CL64 HEK-293T C131(0.85)  LDD1659  [3]
 LDCM0457  CL65 HEK-293T C131(0.95)  LDD1660  [3]
 LDCM0458  CL66 HEK-293T C131(0.97)  LDD1661  [3]
 LDCM0459  CL67 HEK-293T C131(0.96)  LDD1662  [3]
 LDCM0460  CL68 HEK-293T C131(1.03)  LDD1663  [3]
 LDCM0461  CL69 HEK-293T C131(0.97)  LDD1664  [3]
 LDCM0462  CL7 HEK-293T C131(1.08)  LDD1665  [3]
 LDCM0463  CL70 HEK-293T C131(0.96)  LDD1666  [3]
 LDCM0464  CL71 HEK-293T C131(1.13)  LDD1667  [3]
 LDCM0465  CL72 HEK-293T C131(0.99)  LDD1668  [3]
 LDCM0466  CL73 HEK-293T C131(1.04)  LDD1669  [3]
 LDCM0467  CL74 HEK-293T C131(1.12)  LDD1670  [3]
 LDCM0469  CL76 HEK-293T C131(1.13)  LDD1672  [3]
 LDCM0470  CL77 HEK-293T C131(0.96)  LDD1673  [3]
 LDCM0471  CL78 HEK-293T C131(1.05)  LDD1674  [3]
 LDCM0472  CL79 HEK-293T C131(0.97)  LDD1675  [3]
 LDCM0473  CL8 HEK-293T C131(0.77)  LDD1676  [3]
 LDCM0474  CL80 HEK-293T C131(0.92)  LDD1677  [3]
 LDCM0475  CL81 HEK-293T C131(1.22)  LDD1678  [3]
 LDCM0476  CL82 HEK-293T C131(1.01)  LDD1679  [3]
 LDCM0477  CL83 HEK-293T C131(1.24)  LDD1680  [3]
 LDCM0478  CL84 HEK-293T C131(1.10)  LDD1681  [3]
 LDCM0479  CL85 HEK-293T C131(1.12)  LDD1682  [3]
 LDCM0480  CL86 HEK-293T C131(1.00)  LDD1683  [3]
 LDCM0481  CL87 HEK-293T C131(1.08)  LDD1684  [3]
 LDCM0482  CL88 HEK-293T C131(1.12)  LDD1685  [3]
 LDCM0483  CL89 HEK-293T C131(0.85)  LDD1686  [3]
 LDCM0484  CL9 HEK-293T C131(1.03)  LDD1687  [3]
 LDCM0485  CL90 HEK-293T C131(0.82)  LDD1688  [3]
 LDCM0486  CL91 HEK-293T C131(1.06)  LDD1689  [3]
 LDCM0487  CL92 HEK-293T C131(1.06)  LDD1690  [3]
 LDCM0488  CL93 HEK-293T C131(0.91)  LDD1691  [3]
 LDCM0489  CL94 HEK-293T C131(0.97)  LDD1692  [3]
 LDCM0490  CL95 HEK-293T C131(1.13)  LDD1693  [3]
 LDCM0491  CL96 HEK-293T C131(0.84)  LDD1694  [3]
 LDCM0492  CL97 HEK-293T C131(0.95)  LDD1695  [3]
 LDCM0493  CL98 HEK-293T C131(0.91)  LDD1696  [3]
 LDCM0494  CL99 HEK-293T C131(1.05)  LDD1697  [3]
 LDCM0495  E2913 HEK-293T C131(1.12)  LDD1698  [3]
 LDCM0468  Fragment33 HEK-293T C131(1.05)  LDD1671  [3]
 LDCM0427  Fragment51 HEK-293T C131(1.00)  LDD1631  [3]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Transporter and channel
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Importin subunit alpha-3 (KPNA4) Importin alpha family O00629
Nuclear cap-binding protein subunit 1 (NCBP1) NCBP1 family Q09161
Other
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Leukocyte receptor cluster member 8 (LENG8) . Q96PV6

References

1 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
2 An Azo Coupling-Based Chemoproteomic Approach to Systematically Profile the Tyrosine Reactivity in the Human Proteome. Anal Chem. 2021 Jul 27;93(29):10334-10342. doi: 10.1021/acs.analchem.1c01935. Epub 2021 Jul 12.
3 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
4 Global profiling of functional histidines in live cells using small-molecule photosensitizer and chemical probe relay labelling. Nat Chem. 2024 Jun 4. doi: 10.1038/s41557-024-01545-6. Online ahead of print.
Mass spectrometry data entry: PXD042377
5 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
6 Solid Phase Synthesis of Fluorosulfate Containing Macrocycles for Chemoproteomic Workflows. bioRxiv [Preprint]. 2023 Feb 18:2023.02.17.529022. doi: 10.1101/2023.02.17.529022.
Mass spectrometry data entry: PXD039931
7 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587