Details of the Target

General Information of Target

Target ID LDTP06631
Target Name NADH dehydrogenase 1 alpha subcomplex subunit 9, mitochondrial (NDUFA9)
Gene Name NDUFA9
Gene ID 4704
Synonyms
NDUFS2L; NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial; Complex I-39kD; CI-39kD; NADH-ubiquinone oxidoreductase 39 kDa subunit
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MAAAAQSRVVRVLSMSRSAITAIATSVCHGPPCRQLHHALMPHGKGGRSSVSGIVATVFG
ATGFLGRYVVNHLGRMGSQVIIPYRCDKYDIMHLRPMGDLGQLLFLEWDARDKDSIRRVV
QHSNVVINLIGRDWETKNFDFEDVFVKIPQAIAQLSKEAGVEKFIHVSHLNANIKSSSRY
LRNKAVGEKVVRDAFPEAIIVKPSDIFGREDRFLNSFASMHRFGPIPLGSLGWKTVKQPV
YVVDVSKGIVNAVKDPDANGKSFAFVGPSRYLLFHLVKYIFAVAHRLFLPFPLPLFAYRW
VARVFEISPFEPWITRDKVERMHITDMKLPHLPGLEDLGIQATPLELKAIEVLRRHRTYR
WLSAEIEDVKPAKTVNI
Target Bioclass
Enzyme
Family
Complex I NDUFA9 subunit family
Subcellular location
Mitochondrion matrix
Function
Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Required for proper complex I assembly. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Uniprot ID
Q16795
Ensemble ID
ENST00000266544.10
HGNC ID
HGNC:7693
ChEMBL ID
CHEMBL2363065

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 12 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
m-APA
 Probe Info  		10.13  LDD0402  [1]
STPyne
 Probe Info  		K189(1.33); K234(6.82)  LDD0277  [2]
IPM
 Probe Info  		N.A.  LDD0241  [3]
BTD
 Probe Info  		C86(0.88)  LDD2093  [4]
Jackson_14
 Probe Info  		2.63  LDD0123  [5]
DBIA
 Probe Info  		C86(0.93)  LDD1507  [6]
4-Iodoacetamidophenylacetylene
 Probe Info  		N.A.  LDD0038  [7]
IA-alkyne
 Probe Info  		N.A.  LDD0036  [7]
Lodoacetamide azide
 Probe Info  		N.A.  LDD0037  [7]
Ox-W18
 Probe Info  		W313(0.00); W134(0.00)  LDD2175  [8]
Acrolein
 Probe Info  		N.A.  LDD0217  [9]
AOyne
 Probe Info  		6.20  LDD0443  [10]
PAL-AfBPP Probe
Click To Hide/Show 11 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
FFF probe11
 Probe Info  		11.68  LDD0471  [11]
FFF probe13
 Probe Info  		20.00  LDD0475  [11]
FFF probe14
 Probe Info  		20.00  LDD0477  [11]
FFF probe2
 Probe Info  		20.00  LDD0463  [11]
FFF probe3
 Probe Info  		15.05  LDD0464  [11]
JN0003
 Probe Info  		13.71  LDD0469  [11]
STS-1
 Probe Info  		N.A.  LDD0136  [12]
STS-2
 Probe Info  		N.A.  LDD0138  [12]
VE-P
 Probe Info  		N.A.  LDD0396  [13]
DA-2
 Probe Info  		N.A.  LDD0071  [14]
OEA-DA
 Probe Info  		3.13  LDD0046  [15]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0524  2-Cyano-N-(2-morpholin-4-yl-ethyl)-acetamide MDA-MB-231 C86(0.99)  LDD2117  [4]
 LDCM0214  AC1 HEK-293T C86(0.93)  LDD1507  [6]
 LDCM0259  AC14 HEK-293T C86(1.31)  LDD1512  [6]
 LDCM0270  AC15 HEK-293T C86(1.33)  LDD1513  [6]
 LDCM0276  AC17 HEK-293T C86(1.23)  LDD1515  [6]
 LDCM0281  AC21 HEK-293T C86(1.25)  LDD1520  [6]
 LDCM0282  AC22 HEK-293T C86(1.10)  LDD1521  [6]
 LDCM0283  AC23 HEK-293T C86(0.95)  LDD1522  [6]
 LDCM0284  AC24 HEK-293T C86(0.97)  LDD1523  [6]
 LDCM0285  AC25 HEK-293T C86(1.10)  LDD1524  [6]
 LDCM0289  AC29 HEK-293T C86(1.24)  LDD1528  [6]
 LDCM0291  AC30 HEK-293T C86(1.02)  LDD1530  [6]
 LDCM0292  AC31 HEK-293T C86(0.92)  LDD1531  [6]
 LDCM0293  AC32 HEK-293T C86(1.77)  LDD1532  [6]
 LDCM0294  AC33 HEK-293T C86(1.52)  LDD1533  [6]
 LDCM0298  AC37 HEK-293T C86(1.25)  LDD1537  [6]
 LDCM0299  AC38 HEK-293T C86(1.49)  LDD1538  [6]
 LDCM0300  AC39 HEK-293T C86(1.67)  LDD1539  [6]
 LDCM0302  AC40 HEK-293T C86(1.08)  LDD1541  [6]
 LDCM0303  AC41 HEK-293T C86(0.88)  LDD1542  [6]
 LDCM0307  AC45 HEK-293T C86(1.35)  LDD1546  [6]
 LDCM0308  AC46 HEK-293T C86(1.68)  LDD1547  [6]
 LDCM0309  AC47 HEK-293T C86(0.94)  LDD1548  [6]
 LDCM0310  AC48 HEK-293T C86(1.18)  LDD1549  [6]
 LDCM0311  AC49 HEK-293T C86(1.59)  LDD1550  [6]
 LDCM0312  AC5 HEK-293T C86(1.28)  LDD1551  [6]
 LDCM0316  AC53 HEK-293T C86(1.73)  LDD1555  [6]
 LDCM0317  AC54 HEK-293T C86(1.42)  LDD1556  [6]
 LDCM0318  AC55 HEK-293T C86(1.21)  LDD1557  [6]
 LDCM0319  AC56 HEK-293T C86(1.17)  LDD1558  [6]
 LDCM0320  AC57 HEK-293T C86(0.77)  LDD1559  [6]
 LDCM0323  AC6 HEK-293T C86(1.27)  LDD1562  [6]
 LDCM0325  AC61 HEK-293T C86(0.98)  LDD1564  [6]
 LDCM0326  AC62 HEK-293T C86(1.13)  LDD1565  [6]
 LDCM0327  AC63 HEK-293T C86(1.05)  LDD1566  [6]
 LDCM0328  AC64 HEK-293T C86(0.73)  LDD1567  [6]
 LDCM0334  AC7 HEK-293T C86(1.16)  LDD1568  [6]
 LDCM0345  AC8 HEK-293T C86(0.84)  LDD1569  [6]
 LDCM0248  AKOS034007472 HEK-293T C86(1.13)  LDD1511  [6]
 LDCM0356  AKOS034007680 HEK-293T C86(1.23)  LDD1570  [6]
 LDCM0275  AKOS034007705 HEK-293T C86(1.18)  LDD1514  [6]
 LDCM0156  Aniline NCI-H1299 11.35  LDD0403  [1]
 LDCM0108  Chloroacetamide HeLa H43(0.00); H122(0.00); H38(0.00)  LDD0222  [9]
 LDCM0367  CL1 HEK-293T C86(0.86)  LDD1571  [6]
 LDCM0368  CL10 HEK-293T C86(0.44)  LDD1572  [6]
 LDCM0370  CL101 HEK-293T C86(1.01)  LDD1574  [6]
 LDCM0374  CL105 HEK-293T C86(1.01)  LDD1578  [6]
 LDCM0378  CL109 HEK-293T C86(1.08)  LDD1582  [6]
 LDCM0379  CL11 HEK-293T C86(0.33)  LDD1583  [6]
 LDCM0383  CL113 HEK-293T C86(1.12)  LDD1587  [6]
 LDCM0387  CL117 HEK-293T C86(0.95)  LDD1591  [6]
 LDCM0390  CL12 HEK-293T C86(0.56)  LDD1594  [6]
 LDCM0392  CL121 HEK-293T C86(1.18)  LDD1596  [6]
 LDCM0396  CL125 HEK-293T C86(1.10)  LDD1600  [6]
 LDCM0400  CL13 HEK-293T C86(0.99)  LDD1604  [6]
 LDCM0404  CL17 HEK-293T C86(0.65)  LDD1608  [6]
 LDCM0409  CL21 HEK-293T C86(0.53)  LDD1613  [6]
 LDCM0410  CL22 HEK-293T C86(0.40)  LDD1614  [6]
 LDCM0411  CL23 HEK-293T C86(0.31)  LDD1615  [6]
 LDCM0412  CL24 HEK-293T C86(0.37)  LDD1616  [6]
 LDCM0413  CL25 HEK-293T C86(0.75)  LDD1617  [6]
 LDCM0417  CL29 HEK-293T C86(0.97)  LDD1621  [6]
 LDCM0422  CL33 HEK-293T C86(0.51)  LDD1626  [6]
 LDCM0423  CL34 HEK-293T C86(0.35)  LDD1627  [6]
 LDCM0424  CL35 HEK-293T C86(0.28)  LDD1628  [6]
 LDCM0425  CL36 HEK-293T C86(0.54)  LDD1629  [6]
 LDCM0426  CL37 HEK-293T C86(0.98)  LDD1630  [6]
 LDCM0431  CL41 HEK-293T C86(0.95)  LDD1635  [6]
 LDCM0435  CL45 HEK-293T C86(0.61)  LDD1639  [6]
 LDCM0436  CL46 HEK-293T C86(0.39)  LDD1640  [6]
 LDCM0437  CL47 HEK-293T C86(0.31)  LDD1641  [6]
 LDCM0438  CL48 HEK-293T C86(0.31)  LDD1642  [6]
 LDCM0439  CL49 HEK-293T C86(1.01)  LDD1643  [6]
 LDCM0440  CL5 HEK-293T C86(0.83)  LDD1644  [6]
 LDCM0444  CL53 HEK-293T C86(0.81)  LDD1647  [6]
 LDCM0448  CL57 HEK-293T C86(0.58)  LDD1651  [6]
 LDCM0449  CL58 HEK-293T C86(0.45)  LDD1652  [6]
 LDCM0450  CL59 HEK-293T C86(0.45)  LDD1653  [6]
 LDCM0452  CL60 HEK-293T C86(0.40)  LDD1655  [6]
 LDCM0453  CL61 HEK-293T C86(0.87)  LDD1656  [6]
 LDCM0457  CL65 HEK-293T C86(1.17)  LDD1660  [6]
 LDCM0461  CL69 HEK-293T C86(0.72)  LDD1664  [6]
 LDCM0463  CL70 HEK-293T C86(0.52)  LDD1666  [6]
 LDCM0464  CL71 HEK-293T C86(0.51)  LDD1667  [6]
 LDCM0465  CL72 HEK-293T C86(0.43)  LDD1668  [6]
 LDCM0466  CL73 HEK-293T C86(0.83)  LDD1669  [6]
 LDCM0470  CL77 HEK-293T C86(0.40)  LDD1673  [6]
 LDCM0475  CL81 HEK-293T C86(0.80)  LDD1678  [6]
 LDCM0476  CL82 HEK-293T C86(0.64)  LDD1679  [6]
 LDCM0477  CL83 HEK-293T C86(0.51)  LDD1680  [6]
 LDCM0478  CL84 HEK-293T C86(0.66)  LDD1681  [6]
 LDCM0479  CL85 HEK-293T C86(0.78)  LDD1682  [6]
 LDCM0483  CL89 HEK-293T C86(0.68)  LDD1686  [6]
 LDCM0484  CL9 HEK-293T C86(0.50)  LDD1687  [6]
 LDCM0488  CL93 HEK-293T C86(0.78)  LDD1691  [6]
 LDCM0489  CL94 HEK-293T C86(0.64)  LDD1692  [6]
 LDCM0490  CL95 HEK-293T C86(0.67)  LDD1693  [6]
 LDCM0491  CL96 HEK-293T C86(0.90)  LDD1694  [6]
 LDCM0492  CL97 HEK-293T C86(0.82)  LDD1695  [6]
 LDCM0107  IAA HeLa N.A.  LDD0221  [9]
 LDCM0528  N-(4-bromophenyl)-2-cyano-N-phenylacetamide MDA-MB-231 C86(0.82)  LDD2121  [4]
 LDCM0109  NEM HeLa H122(0.00); H38(0.00)  LDD0223  [9]
 LDCM0500  Nucleophilic fragment 13a MDA-MB-231 C86(0.88)  LDD2093  [4]
 LDCM0506  Nucleophilic fragment 16a MDA-MB-231 C86(0.86)  LDD2099  [4]
 LDCM0514  Nucleophilic fragment 20a MDA-MB-231 C86(1.10)  LDD2107  [4]
 LDCM0515  Nucleophilic fragment 20b MDA-MB-231 C86(0.94)  LDD2108  [4]
 LDCM0516  Nucleophilic fragment 21a MDA-MB-231 C86(0.67)  LDD2109  [4]
 LDCM0518  Nucleophilic fragment 22a MDA-MB-231 C86(1.01)  LDD2111  [4]
 LDCM0526  Nucleophilic fragment 26a MDA-MB-231 C86(2.10)  LDD2119  [4]
 LDCM0532  Nucleophilic fragment 29a MDA-MB-231 C86(0.80)  LDD2125  [4]
 LDCM0536  Nucleophilic fragment 31 MDA-MB-231 C86(1.21)  LDD2129  [4]
 LDCM0543  Nucleophilic fragment 38 MDA-MB-231 C86(1.21)  LDD2136  [4]
 LDCM0550  Nucleophilic fragment 5a MDA-MB-231 C86(1.90)  LDD2144  [4]
 LDCM0552  Nucleophilic fragment 6a MDA-MB-231 C86(0.89)  LDD2146  [4]
 LDCM0559  Nucleophilic fragment 9b MDA-MB-231 C86(1.01)  LDD2153  [4]
 LDCM0016  Ranjitkar_cp1 MDA-MB-231 2.63  LDD0123  [5]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Nitric oxide-associated protein 1 (NOA1) TRAFAC class YlqF/YawG GTPase family Q8NC60
Transporter and channel
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Huntingtin (HTT) Huntingtin family P42858
Other
Click To Hide/Show 3 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Biogenesis of lysosome-related organelles complex 1 subunit 1 (BLOC1S1) BLOC1S1 family P78537
Cysteine-rich tail protein 1 (CYSRT1) CYSRT1 family A8MQ03
Keratin-associated protein 6-2 (KRTAP6-2) KRTAP type 6 family Q3LI66

The Drug(s) Related To This Target

Approved
Click To Hide/Show 2 Drug(s) Interacting with This Target
Drug Name Drug Type External ID
Flavin Adenine Dinucleotide Small molecular drug DB03147
Nadh Small molecular drug DB00157

References

1 Quantitative and Site-Specific Chemoproteomic Profiling of Targets of Acrolein. Chem Res Toxicol. 2019 Mar 18;32(3):467-473. doi: 10.1021/acs.chemrestox.8b00343. Epub 2019 Jan 15.
2 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
3 Oxidant-Induced Bioconjugation for Protein Labeling in Live Cells. ACS Chem Biol. 2023 Jan 20;18(1):112-122. doi: 10.1021/acschembio.2c00740. Epub 2022 Dec 21.
4 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
5 Appendage and Scaffold Diverse Fully Functionalized Small-Molecule Probes via a Minimalist Terminal Alkyne-Aliphatic Diazirine Isocyanide. J Org Chem. 2018 Sep 21;83(18):11245-11253. doi: 10.1021/acs.joc.8b01831. Epub 2018 Aug 31.
6 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
7 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
8 Oxidative cyclization reagents reveal tryptophan cation- interactions. Nature. 2024 Mar;627(8004):680-687. doi: 10.1038/s41586-024-07140-6. Epub 2024 Mar 6.
Mass spectrometry data entry: PXD001377 , PXD005252
9 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
10 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
11 Ligand and Target Discovery by Fragment-Based Screening in Human Cells. Cell. 2017 Jan 26;168(3):527-541.e29. doi: 10.1016/j.cell.2016.12.029. Epub 2017 Jan 19.
12 Design and synthesis of minimalist terminal alkyne-containing diazirine photo-crosslinkers and their incorporation into kinase inhibitors for cell- and tissue-based proteome profiling. Angew Chem Int Ed Engl. 2013 Aug 12;52(33):8551-6. doi: 10.1002/anie.201300683. Epub 2013 Jun 10.
13 Pharmacological Targeting of Vacuolar H(+)-ATPase via Subunit V1G Combats Multidrug-Resistant Cancer. Cell Chem Biol. 2020 Nov 19;27(11):1359-1370.e8. doi: 10.1016/j.chembiol.2020.06.011. Epub 2020 Jul 9.
14 Cell-based proteome profiling of potential dasatinib targets by use of affinity-based probes. J Am Chem Soc. 2012 Feb 15;134(6):3001-14. doi: 10.1021/ja208518u. Epub 2012 Feb 1.
15 Mapping Protein Targets of Bioactive Small Molecules Using Lipid-Based Chemical Proteomics. ACS Chem Biol. 2017 Oct 20;12(10):2671-2681. doi: 10.1021/acschembio.7b00581. Epub 2017 Sep 20.
Mass spectrometry data entry: PXD007570