Details of the Target

General Information of Target

Target ID LDTP00830
Target Name BUB3-interacting and GLEBS motif-containing protein ZNF207 (ZNF207)
Gene Name ZNF207
Gene ID 7756
Synonyms
BUGZ; BUB3-interacting and GLEBS motif-containing protein ZNF207; BuGZ; hBuGZ; Zinc finger protein 207
3D Structure
Download
2D Sequence (FASTA)
Download
3D Structure (PDB)
Download
Sequence
MGRKKKKQLKPWCWYCNRDFDDEKILIQHQKAKHFKCHICHKKLYTGPGLAIHCMQVHKE
TIDAVPNAIPGRTDIELEIYGMEGIPEKDMDERRRLLEQKTQESQKKKQQDDSDEYDDDD
SAASTSFQPQPVQPQQGYIPPMAQPGLPPVPGAPGMPPGIPPLMPGVPPLMPGMPPVMPG
MPPGMMPMGGMMPPGPGIPPLMPGMPPGMPPPVPRPGIPPMTQAQAVSAPGILNRPPAPT
ATVPAPQPPVTKPLFPSAGQMGTPVTSSSTASSNSESLSASSKALFPSTAQAQAAVQGPV
GTDFKPLNSTPATTTEPPKPTFPAYTQSTASTTSTTNSTAAKPAASITSKPATLTTTSAT
SKLIHPDEDISLEERRAQLPKYQRNLPRPGQAPIGNPPVGPIGGMMPPQPGIPQQQGMRP
PMPPHGQYGGHHQGMPGYLPGAMPPYGQGPPMVPPYQGGPPRPPMGMRPPVMSQGGRY
Target Bioclass
Other
Subcellular location
Nucleus
Function
Kinetochore- and microtubule-binding protein that plays a key role in spindle assembly. ZNF207/BuGZ is mainly composed of disordered low-complexity regions and undergoes phase transition or coacervation to form temperature-dependent liquid droplets. Coacervation promotes microtubule bundling and concentrates tubulin, promoting microtubule polymerization and assembly of spindle and spindle matrix by concentrating its building blocks. Also acts as a regulator of mitotic chromosome alignment by mediating the stability and kinetochore loading of BUB3. Mechanisms by which BUB3 is protected are unclear: according to a first report, ZNF207/BuGZ may act by blocking ubiquitination and proteasomal degradation of BUB3. According to another report, the stabilization is independent of the proteasome.
Uniprot ID
O43670
Ensemble ID
ENST00000321233.10
HGNC ID
HGNC:12998

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 17 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
CY-1
 Probe Info  		100.00  LDD0243  [1]
YN-1
 Probe Info  		100.00  LDD0444  [2]
STPyne
 Probe Info  		K31(9.67); K33(10.00); K381(9.09)  LDD0277  [3]
Probe 1
 Probe Info  		Y80(25.64)  LDD3495  [4]
DBIA
 Probe Info  		C54(1.27)  LDD3310  [5]
BTD
 Probe Info  		C54(0.90)  LDD2109  [6]
HHS-475
 Probe Info  		Y45(0.34)  LDD0264  [7]
ATP probe
 Probe Info  		K31(0.00); K33(0.00); K24(0.00); K43(0.00)  LDD0199  [8]
4-Iodoacetamidophenylacetylene
 Probe Info  		N.A.  LDD0038  [9]
IA-alkyne
 Probe Info  		C13(0.00); C16(0.00)  LDD0036  [9]
Lodoacetamide azide
 Probe Info  		N.A.  LDD0037  [9]
NHS
 Probe Info  		N.A.  LDD0010  [10]
SF
 Probe Info  		N.A.  LDD0028  [11]
Acrolein
 Probe Info  		N.A.  LDD0217  [12]
Methacrolein
 Probe Info  		N.A.  LDD0218  [12]
HHS-465
 Probe Info  		N.A.  LDD2240  [13]
HHS-482
 Probe Info  		Y45(0.73)  LDD2239  [14]
PAL-AfBPP Probe
Click To Hide/Show 3 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C187
 Probe Info  		18.25  LDD1865  [15]
C191
 Probe Info  		17.39  LDD1868  [15]
C193
 Probe Info  		9.00  LDD1869  [15]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0226  AC11 HEK-293T C13(0.97)  LDD1509  [16]
 LDCM0237  AC12 HEK-293T C13(0.96)  LDD1510  [16]
 LDCM0270  AC15 HEK-293T C13(0.89)  LDD1513  [16]
 LDCM0278  AC19 HEK-293T C13(0.85)  LDD1517  [16]
 LDCM0280  AC20 HEK-293T C13(1.01)  LDD1519  [16]
 LDCM0283  AC23 HEK-293T C13(0.95)  LDD1522  [16]
 LDCM0287  AC27 HEK-293T C13(0.89)  LDD1526  [16]
 LDCM0288  AC28 HEK-293T C13(1.04)  LDD1527  [16]
 LDCM0290  AC3 HEK-293T C13(1.00)  LDD1529  [16]
 LDCM0292  AC31 HEK-293T C13(0.97)  LDD1531  [16]
 LDCM0296  AC35 HEK-293T C13(0.99)  LDD1535  [16]
 LDCM0297  AC36 HEK-293T C13(1.09)  LDD1536  [16]
 LDCM0300  AC39 HEK-293T C13(1.06)  LDD1539  [16]
 LDCM0301  AC4 HEK-293T C13(1.10)  LDD1540  [16]
 LDCM0305  AC43 HEK-293T C13(0.88)  LDD1544  [16]
 LDCM0306  AC44 HEK-293T C13(1.03)  LDD1545  [16]
 LDCM0309  AC47 HEK-293T C13(0.97)  LDD1548  [16]
 LDCM0314  AC51 HEK-293T C13(1.03)  LDD1553  [16]
 LDCM0315  AC52 HEK-293T C13(1.04)  LDD1554  [16]
 LDCM0318  AC55 HEK-293T C13(1.03)  LDD1557  [16]
 LDCM0322  AC59 HEK-293T C13(0.91)  LDD1561  [16]
 LDCM0324  AC60 HEK-293T C13(0.88)  LDD1563  [16]
 LDCM0327  AC63 HEK-293T C13(0.85)  LDD1566  [16]
 LDCM0334  AC7 HEK-293T C13(0.88)  LDD1568  [16]
 LDCM0108  Chloroacetamide HeLa N.A.  LDD0222  [12]
 LDCM0369  CL100 HEK-293T C13(1.12)  LDD1573  [16]
 LDCM0371  CL102 HEK-293T C13(0.93)  LDD1575  [16]
 LDCM0372  CL103 HEK-293T C13(0.95)  LDD1576  [16]
 LDCM0373  CL104 HEK-293T C13(1.04)  LDD1577  [16]
 LDCM0375  CL106 HEK-293T C13(0.96)  LDD1579  [16]
 LDCM0376  CL107 HEK-293T C13(0.98)  LDD1580  [16]
 LDCM0377  CL108 HEK-293T C13(1.04)  LDD1581  [16]
 LDCM0379  CL11 HEK-293T C13(0.95)  LDD1583  [16]
 LDCM0380  CL110 HEK-293T C13(0.85)  LDD1584  [16]
 LDCM0381  CL111 HEK-293T C13(0.85)  LDD1585  [16]
 LDCM0382  CL112 HEK-293T C13(0.92)  LDD1586  [16]
 LDCM0384  CL114 HEK-293T C13(0.94)  LDD1588  [16]
 LDCM0385  CL115 HEK-293T C13(0.98)  LDD1589  [16]
 LDCM0386  CL116 HEK-293T C13(1.15)  LDD1590  [16]
 LDCM0388  CL118 HEK-293T C13(0.94)  LDD1592  [16]
 LDCM0389  CL119 HEK-293T C13(0.98)  LDD1593  [16]
 LDCM0391  CL120 HEK-293T C13(1.13)  LDD1595  [16]
 LDCM0393  CL122 HEK-293T C13(0.93)  LDD1597  [16]
 LDCM0394  CL123 HEK-293T C13(0.97)  LDD1598  [16]
 LDCM0395  CL124 HEK-293T C13(1.12)  LDD1599  [16]
 LDCM0397  CL126 HEK-293T C13(0.83)  LDD1601  [16]
 LDCM0398  CL127 HEK-293T C13(1.00)  LDD1602  [16]
 LDCM0399  CL128 HEK-293T C13(1.06)  LDD1603  [16]
 LDCM0401  CL14 HEK-293T C13(1.10)  LDD1605  [16]
 LDCM0402  CL15 HEK-293T C13(1.19)  LDD1606  [16]
 LDCM0403  CL16 HEK-293T C13(1.15)  LDD1607  [16]
 LDCM0406  CL19 HEK-293T C13(1.21)  LDD1610  [16]
 LDCM0407  CL2 HEK-293T C13(1.00)  LDD1611  [16]
 LDCM0408  CL20 HEK-293T C13(1.18)  LDD1612  [16]
 LDCM0411  CL23 HEK-293T C13(1.12)  LDD1615  [16]
 LDCM0414  CL26 HEK-293T C13(1.11)  LDD1618  [16]
 LDCM0415  CL27 HEK-293T C13(1.01)  LDD1619  [16]
 LDCM0416  CL28 HEK-293T C13(1.09)  LDD1620  [16]
 LDCM0418  CL3 HEK-293T C13(1.10)  LDD1622  [16]
 LDCM0420  CL31 HEK-293T C13(1.07)  LDD1624  [16]
 LDCM0421  CL32 HEK-293T C13(1.02)  LDD1625  [16]
 LDCM0424  CL35 HEK-293T C13(0.88)  LDD1628  [16]
 LDCM0428  CL39 HEK-293T C13(1.08)  LDD1632  [16]
 LDCM0429  CL4 HEK-293T C13(1.14)  LDD1633  [16]
 LDCM0430  CL40 HEK-293T C13(0.98)  LDD1634  [16]
 LDCM0433  CL43 HEK-293T C13(0.86)  LDD1637  [16]
 LDCM0434  CL44 HEK-293T C13(0.95)  LDD1638  [16]
 LDCM0437  CL47 HEK-293T C13(0.90)  LDD1641  [16]
 LDCM0441  CL50 HEK-293T C13(1.05)  LDD1645  [16]
 LDCM0443  CL52 HEK-293T C13(1.01)  LDD1646  [16]
 LDCM0446  CL55 HEK-293T C13(0.88)  LDD1649  [16]
 LDCM0447  CL56 HEK-293T C13(0.98)  LDD1650  [16]
 LDCM0450  CL59 HEK-293T C13(0.89)  LDD1653  [16]
 LDCM0454  CL62 HEK-293T C13(1.08)  LDD1657  [16]
 LDCM0455  CL63 HEK-293T C13(1.09)  LDD1658  [16]
 LDCM0456  CL64 HEK-293T C13(1.00)  LDD1659  [16]
 LDCM0459  CL67 HEK-293T C13(0.97)  LDD1662  [16]
 LDCM0460  CL68 HEK-293T C13(1.05)  LDD1663  [16]
 LDCM0462  CL7 HEK-293T C13(1.04)  LDD1665  [16]
 LDCM0464  CL71 HEK-293T C13(0.93)  LDD1667  [16]
 LDCM0467  CL74 HEK-293T C13(1.12)  LDD1670  [16]
 LDCM0469  CL76 HEK-293T C13(1.07)  LDD1672  [16]
 LDCM0472  CL79 HEK-293T C13(0.97)  LDD1675  [16]
 LDCM0473  CL8 HEK-293T C13(0.90)  LDD1676  [16]
 LDCM0474  CL80 HEK-293T C13(1.15)  LDD1677  [16]
 LDCM0477  CL83 HEK-293T C13(0.92)  LDD1680  [16]
 LDCM0480  CL86 HEK-293T C13(1.12)  LDD1683  [16]
 LDCM0481  CL87 HEK-293T C13(1.00)  LDD1684  [16]
 LDCM0482  CL88 HEK-293T C13(1.12)  LDD1685  [16]
 LDCM0486  CL91 HEK-293T C13(1.05)  LDD1689  [16]
 LDCM0487  CL92 HEK-293T C13(1.11)  LDD1690  [16]
 LDCM0490  CL95 HEK-293T C13(0.98)  LDD1693  [16]
 LDCM0493  CL98 HEK-293T C13(0.92)  LDD1696  [16]
 LDCM0494  CL99 HEK-293T C13(0.98)  LDD1697  [16]
 LDCM0495  E2913 HEK-293T C13(1.03)  LDD1698  [16]
 LDCM0468  Fragment33 HEK-293T C13(1.16)  LDD1671  [16]
 LDCM0427  Fragment51 HEK-293T C13(1.04)  LDD1631  [16]
 LDCM0116  HHS-0101 DM93 Y45(0.34)  LDD0264  [7]
 LDCM0117  HHS-0201 DM93 Y45(0.06)  LDD0265  [7]
 LDCM0118  HHS-0301 DM93 Y45(1.57)  LDD0266  [7]
 LDCM0119  HHS-0401 DM93 Y45(0.57)  LDD0267  [7]
 LDCM0120  HHS-0701 DM93 Y45(1.00)  LDD0268  [7]
 LDCM0107  IAA HeLa N.A.  LDD0221  [12]
 LDCM0022  KB02 HEK-293T C13(0.98)  LDD1492  [16]
 LDCM0023  KB03 HEK-293T C13(1.03)  LDD1497  [16]
 LDCM0024  KB05 COLO792 C54(1.27)  LDD3310  [5]
 LDCM0109  NEM HeLa H365(0.00); C54(0.00)  LDD0223  [12]
 LDCM0516  Nucleophilic fragment 21a MDA-MB-231 C54(0.90)  LDD2109  [6]
 LDCM0527  Nucleophilic fragment 26b MDA-MB-231 C37(1.04)  LDD2120  [6]
 LDCM0530  Nucleophilic fragment 28a MDA-MB-231 C37(0.56); C54(0.89)  LDD2123  [6]
 LDCM0540  Nucleophilic fragment 35 MDA-MB-231 C37(0.91)  LDD2133  [6]
 LDCM0541  Nucleophilic fragment 36 MDA-MB-231 C37(0.91)  LDD2134  [6]
 LDCM0543  Nucleophilic fragment 38 MDA-MB-231 C54(1.02)  LDD2136  [6]
 LDCM0549  Nucleophilic fragment 43 MDA-MB-231 C37(0.79)  LDD2143  [6]

References

1 Cyclopropenone, Cyclopropeniminium Ion, and Cyclopropenethione as Novel Electrophilic Warheads for Potential Target Discovery of Triple-Negative Breast Cancer. J Med Chem. 2023 Feb 23;66(4):2851-2864. doi: 10.1021/acs.jmedchem.2c01889. Epub 2023 Feb 10.
2 Ynamide Electrophile for the Profiling of Ligandable Carboxyl Residues in Live Cells and the Development of New Covalent Inhibitors. J Med Chem. 2022 Aug 11;65(15):10408-10418. doi: 10.1021/acs.jmedchem.2c00272. Epub 2022 Jul 26.
3 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
4 An Azo Coupling-Based Chemoproteomic Approach to Systematically Profile the Tyrosine Reactivity in the Human Proteome. Anal Chem. 2021 Jul 27;93(29):10334-10342. doi: 10.1021/acs.analchem.1c01935. Epub 2021 Jul 12.
5 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
6 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
7 Discovery of a Cell-Active SuTEx Ligand of Prostaglandin Reductase 2. Chembiochem. 2021 Jun 15;22(12):2134-2139. doi: 10.1002/cbic.202000879. Epub 2021 Apr 29.
8 Targeted Proteomic Approaches for Proteome-Wide Characterizations of the AMP-Binding Capacities of Kinases. J Proteome Res. 2022 Aug 5;21(8):2063-2070. doi: 10.1021/acs.jproteome.2c00225. Epub 2022 Jul 12.
9 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
10 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
11 Solid Phase Synthesis of Fluorosulfate Containing Macrocycles for Chemoproteomic Workflows. bioRxiv [Preprint]. 2023 Feb 18:2023.02.17.529022. doi: 10.1101/2023.02.17.529022.
Mass spectrometry data entry: PXD039931
12 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
13 Global profiling identifies a stress-responsive tyrosine site on EDC3 regulating biomolecular condensate formation. Cell Chem Biol. 2022 Dec 15;29(12):1709-1720.e7. doi: 10.1016/j.chembiol.2022.11.008. Epub 2022 Dec 6.
Mass spectrometry data entry: PXD038010
14 Global targeting of functional tyrosines using sulfur-triazole exchange chemistry. Nat Chem Biol. 2020 Feb;16(2):150-159. doi: 10.1038/s41589-019-0404-5. Epub 2019 Nov 25.
15 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587
16 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402