Details of the Target

General Information of Target

Target ID LDTP00635
Target Name Monocarboxylate transporter 4 (SLC16A3)
Gene Name SLC16A3
Gene ID 9123
Synonyms
MCT3; MCT4; Monocarboxylate transporter 4; MCT 4; Solute carrier family 16 member 3
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MGGAVVDEGPTGVKAPDGGWGWAVLFGCFVITGFSYAFPKAVSVFFKELIQEFGIGYSDT
AWISSILLAMLYGTGPLCSVCVNRFGCRPVMLVGGLFASLGMVAASFCRSIIQVYLTTGV
ITGLGLALNFQPSLIMLNRYFSKRRPMANGLAAAGSPVFLCALSPLGQLLQDRYGWRGGF
LILGGLLLNCCVCAALMRPLVVTAQPGSGPPRPSRRLLDLSVFRDRGFVLYAVAASVMVL
GLFVPPVFVVSYAKDLGVPDTKAAFLLTILGFIDIFARPAAGFVAGLGKVRPYSVYLFSF
SMFFNGLADLAGSTAGDYGGLVVFCIFFGISYGMVGALQFEVLMAIVGTHKFSSAIGLVL
LMEAVAVLVGPPSGGKLLDATHVYMYVFILAGAEVLTSSLILLLGNFFCIRKKPKEPQPE
VAAAEEEKLHKPPADSGVDLREVEHFLKAEPEKNGEVVHTPETSV
Target Type
Literature-reported
Target Bioclass
Transporter and channel
Family
Major facilitator superfamily, Monocarboxylate porter (TC 2.A.1.13) family
Subcellular location
Cell membrane
Function Proton-dependent transporter of monocarboxylates such as L-lactate and pyruvate. Plays a predominant role in L-lactate efflux from highly glycolytic cells.
TTD ID
T31788
Uniprot ID
O15427
DrugMap ID
TTG6VD5
Ensemble ID
ENST00000392339.6
HGNC ID
HGNC:10924
ChEMBL ID
CHEMBL2073663

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 12 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
CY4
 Probe Info  		100.00  LDD0244  [1]
YN-1
 Probe Info  		100.00  LDD0444  [2]
YN-4
 Probe Info  		100.00  LDD0445  [2]
STPyne
 Probe Info  		K448(7.29)  LDD0277  [3]
IPM
 Probe Info  		N.A.  LDD0241  [4]
DBIA
 Probe Info  		C161(0.70)  LDD0531  [5]
m-APA
 Probe Info  		N.A.  LDD2231  [6]
IA-alkyne
 Probe Info  		N.A.  LDD0162  [7]
NAIA_4
 Probe Info  		N.A.  LDD2226  [8]
TFBX
 Probe Info  		N.A.  LDD0148  [9]
Acrolein
 Probe Info  		N.A.  LDD0217  [10]
W1
 Probe Info  		N.A.  LDD0236  [4]
PAL-AfBPP Probe
Click To Hide/Show 3 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
STS-2
 Probe Info  		N.A.  LDD0138  [11]
VE-P
 Probe Info  		N.A.  LDD0396  [12]
STS-1
 Probe Info  		N.A.  LDD0068  [13]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0214  AC1 HCT 116 C161(0.70)  LDD0531  [5]
 LDCM0215  AC10 HCT 116 C161(0.74)  LDD0532  [5]
 LDCM0226  AC11 HCT 116 C161(0.78)  LDD0543  [5]
 LDCM0230  AC113 HCT 116 C161(0.65)  LDD0547  [5]
 LDCM0231  AC114 HCT 116 C161(0.76)  LDD0548  [5]
 LDCM0232  AC115 HCT 116 C161(0.73)  LDD0549  [5]
 LDCM0233  AC116 HCT 116 C161(0.62)  LDD0550  [5]
 LDCM0234  AC117 HCT 116 C161(0.79)  LDD0551  [5]
 LDCM0235  AC118 HCT 116 C161(0.80)  LDD0552  [5]
 LDCM0236  AC119 HCT 116 C161(0.81)  LDD0553  [5]
 LDCM0237  AC12 HCT 116 C161(0.77)  LDD0554  [5]
 LDCM0238  AC120 HCT 116 C161(0.70)  LDD0555  [5]
 LDCM0239  AC121 HCT 116 C161(0.67)  LDD0556  [5]
 LDCM0240  AC122 HCT 116 C161(0.67)  LDD0557  [5]
 LDCM0241  AC123 HCT 116 C161(0.91)  LDD0558  [5]
 LDCM0242  AC124 HCT 116 C161(0.68)  LDD0559  [5]
 LDCM0243  AC125 HCT 116 C161(0.78)  LDD0560  [5]
 LDCM0244  AC126 HCT 116 C161(0.71)  LDD0561  [5]
 LDCM0245  AC127 HCT 116 C161(0.58)  LDD0562  [5]
 LDCM0246  AC128 HCT 116 C161(1.19)  LDD0563  [5]
 LDCM0247  AC129 HCT 116 C161(0.74)  LDD0564  [5]
 LDCM0249  AC130 HCT 116 C161(1.05)  LDD0566  [5]
 LDCM0250  AC131 HCT 116 C161(1.98)  LDD0567  [5]
 LDCM0251  AC132 HCT 116 C161(0.84)  LDD0568  [5]
 LDCM0252  AC133 HCT 116 C161(0.62)  LDD0569  [5]
 LDCM0253  AC134 HCT 116 C161(1.07)  LDD0570  [5]
 LDCM0254  AC135 HCT 116 C161(0.87)  LDD0571  [5]
 LDCM0255  AC136 HCT 116 C161(1.00)  LDD0572  [5]
 LDCM0256  AC137 HCT 116 C161(0.80)  LDD0573  [5]
 LDCM0257  AC138 HCT 116 C161(0.78)  LDD0574  [5]
 LDCM0258  AC139 HCT 116 C161(0.67)  LDD0575  [5]
 LDCM0259  AC14 HCT 116 C161(0.77)  LDD0576  [5]
 LDCM0260  AC140 HCT 116 C161(0.95)  LDD0577  [5]
 LDCM0261  AC141 HCT 116 C161(0.99)  LDD0578  [5]
 LDCM0262  AC142 HCT 116 C161(0.94)  LDD0579  [5]
 LDCM0270  AC15 HCT 116 C161(0.83)  LDD0587  [5]
 LDCM0279  AC2 HCT 116 C161(0.66)  LDD0596  [5]
 LDCM0290  AC3 HCT 116 C161(0.70)  LDD0607  [5]
 LDCM0301  AC4 HCT 116 C161(0.65)  LDD0618  [5]
 LDCM0308  AC46 HCT 116 C161(1.18)  LDD0625  [5]
 LDCM0309  AC47 HCT 116 C161(1.24)  LDD0626  [5]
 LDCM0310  AC48 HCT 116 C161(1.06)  LDD0627  [5]
 LDCM0311  AC49 HCT 116 C161(1.15)  LDD0628  [5]
 LDCM0312  AC5 HCT 116 C161(0.61)  LDD0629  [5]
 LDCM0313  AC50 HCT 116 C161(1.23)  LDD0630  [5]
 LDCM0314  AC51 HCT 116 C161(1.31)  LDD0631  [5]
 LDCM0315  AC52 HCT 116 C161(1.19)  LDD0632  [5]
 LDCM0316  AC53 HCT 116 C161(1.31)  LDD0633  [5]
 LDCM0317  AC54 HCT 116 C161(1.86)  LDD0634  [5]
 LDCM0318  AC55 HCT 116 C161(1.08)  LDD0635  [5]
 LDCM0319  AC56 HCT 116 C161(0.94)  LDD0636  [5]
 LDCM0323  AC6 HCT 116 C161(0.91)  LDD0640  [5]
 LDCM0334  AC7 HCT 116 C161(0.83)  LDD0651  [5]
 LDCM0345  AC8 HCT 116 C161(0.70)  LDD0662  [5]
 LDCM0349  AC83 HCT 116 C161(0.92)  LDD0666  [5]
 LDCM0350  AC84 HCT 116 C161(0.88)  LDD0667  [5]
 LDCM0351  AC85 HCT 116 C161(0.73)  LDD0668  [5]
 LDCM0352  AC86 HCT 116 C161(0.90)  LDD0669  [5]
 LDCM0353  AC87 HCT 116 C161(0.80)  LDD0670  [5]
 LDCM0354  AC88 HCT 116 C161(0.69)  LDD0671  [5]
 LDCM0355  AC89 HCT 116 C161(0.83)  LDD0672  [5]
 LDCM0357  AC90 HCT 116 C161(0.94)  LDD0674  [5]
 LDCM0358  AC91 HCT 116 C161(0.83)  LDD0675  [5]
 LDCM0359  AC92 HCT 116 C161(0.89)  LDD0676  [5]
 LDCM0360  AC93 HCT 116 C161(0.79)  LDD0677  [5]
 LDCM0361  AC94 HCT 116 C161(0.96)  LDD0678  [5]
 LDCM0362  AC95 HCT 116 C161(0.85)  LDD0679  [5]
 LDCM0363  AC96 HCT 116 C161(0.84)  LDD0680  [5]
 LDCM0364  AC97 HCT 116 C161(0.96)  LDD0681  [5]
 LDCM0248  AKOS034007472 HCT 116 C161(0.79)  LDD0565  [5]
 LDCM0356  AKOS034007680 HCT 116 C161(0.71)  LDD0673  [5]
 LDCM0275  AKOS034007705 HCT 116 C161(0.75)  LDD0592  [5]
 LDCM0108  Chloroacetamide HeLa N.A.  LDD0222  [10]
 LDCM0367  CL1 HCT 116 C161(1.06)  LDD0684  [5]
 LDCM0368  CL10 HCT 116 C161(1.02)  LDD0685  [5]
 LDCM0369  CL100 HCT 116 C161(0.73)  LDD0686  [5]
 LDCM0370  CL101 HCT 116 C161(0.68)  LDD0687  [5]
 LDCM0371  CL102 HCT 116 C161(0.68)  LDD0688  [5]
 LDCM0372  CL103 HCT 116 C161(0.81)  LDD0689  [5]
 LDCM0373  CL104 HCT 116 C161(0.79)  LDD0690  [5]
 LDCM0379  CL11 HCT 116 C161(1.14)  LDD0696  [5]
 LDCM0390  CL12 HCT 116 C161(0.87)  LDD0707  [5]
 LDCM0392  CL121 HCT 116 C161(0.92)  LDD0709  [5]
 LDCM0393  CL122 HCT 116 C161(1.14)  LDD0710  [5]
 LDCM0394  CL123 HCT 116 C161(1.17)  LDD0711  [5]
 LDCM0395  CL124 HCT 116 C161(1.22)  LDD0712  [5]
 LDCM0400  CL13 HCT 116 C161(1.07)  LDD0717  [5]
 LDCM0401  CL14 HCT 116 C161(1.15)  LDD0718  [5]
 LDCM0402  CL15 HCT 116 C161(1.25)  LDD0719  [5]
 LDCM0403  CL16 HCT 116 C161(0.76)  LDD0720  [5]
 LDCM0404  CL17 HCT 116 C161(0.77)  LDD0721  [5]
 LDCM0405  CL18 HCT 116 C161(0.91)  LDD0722  [5]
 LDCM0406  CL19 HCT 116 C161(0.76)  LDD0723  [5]
 LDCM0407  CL2 HCT 116 C161(1.09)  LDD0724  [5]
 LDCM0408  CL20 HCT 116 C161(0.80)  LDD0725  [5]
 LDCM0409  CL21 HCT 116 C161(0.78)  LDD0726  [5]
 LDCM0410  CL22 HCT 116 C161(0.87)  LDD0727  [5]
 LDCM0411  CL23 HCT 116 C161(1.12)  LDD0728  [5]
 LDCM0412  CL24 HCT 116 C161(0.71)  LDD0729  [5]
 LDCM0413  CL25 HCT 116 C161(0.81)  LDD0730  [5]
 LDCM0414  CL26 HCT 116 C161(0.90)  LDD0731  [5]
 LDCM0415  CL27 HCT 116 C161(0.74)  LDD0732  [5]
 LDCM0416  CL28 HCT 116 C161(0.81)  LDD0733  [5]
 LDCM0417  CL29 HCT 116 C161(0.76)  LDD0734  [5]
 LDCM0418  CL3 HCT 116 C161(0.94)  LDD0735  [5]
 LDCM0419  CL30 HCT 116 C161(0.84)  LDD0736  [5]
 LDCM0429  CL4 HCT 116 C161(1.23)  LDD0746  [5]
 LDCM0440  CL5 HCT 116 C161(1.22)  LDD0757  [5]
 LDCM0451  CL6 HCT 116 C161(1.05)  LDD0768  [5]
 LDCM0462  CL7 HCT 116 C161(1.03)  LDD0779  [5]
 LDCM0469  CL76 HCT 116 C161(0.88)  LDD0786  [5]
 LDCM0470  CL77 HCT 116 C161(1.33)  LDD0787  [5]
 LDCM0471  CL78 HCT 116 C161(0.73)  LDD0788  [5]
 LDCM0472  CL79 HCT 116 C161(1.13)  LDD0789  [5]
 LDCM0473  CL8 HCT 116 C161(0.99)  LDD0790  [5]
 LDCM0474  CL80 HCT 116 C161(0.84)  LDD0791  [5]
 LDCM0475  CL81 HCT 116 C161(0.74)  LDD0792  [5]
 LDCM0476  CL82 HCT 116 C161(0.94)  LDD0793  [5]
 LDCM0477  CL83 HCT 116 C161(0.92)  LDD0794  [5]
 LDCM0478  CL84 HCT 116 C161(0.91)  LDD0795  [5]
 LDCM0479  CL85 HCT 116 C161(0.83)  LDD0796  [5]
 LDCM0480  CL86 HCT 116 C161(0.75)  LDD0797  [5]
 LDCM0481  CL87 HCT 116 C161(0.82)  LDD0798  [5]
 LDCM0482  CL88 HCT 116 C161(0.80)  LDD0799  [5]
 LDCM0483  CL89 HCT 116 C161(0.86)  LDD0800  [5]
 LDCM0484  CL9 HCT 116 C161(1.24)  LDD0801  [5]
 LDCM0485  CL90 HCT 116 C161(0.96)  LDD0802  [5]
 LDCM0486  CL91 HCT 116 C161(0.67)  LDD0803  [5]
 LDCM0487  CL92 HCT 116 C161(0.65)  LDD0804  [5]
 LDCM0488  CL93 HCT 116 C161(0.68)  LDD0805  [5]
 LDCM0489  CL94 HCT 116 C161(0.73)  LDD0806  [5]
 LDCM0490  CL95 HCT 116 C161(0.58)  LDD0807  [5]
 LDCM0491  CL96 HCT 116 C161(0.62)  LDD0808  [5]
 LDCM0492  CL97 HCT 116 C161(0.68)  LDD0809  [5]
 LDCM0493  CL98 HCT 116 C161(0.61)  LDD0810  [5]
 LDCM0494  CL99 HCT 116 C161(0.66)  LDD0811  [5]
 LDCM0107  IAA HeLa N.A.  LDD0221  [10]
 LDCM0109  NEM HeLa N.A.  LDD0223  [10]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Transporter and channel
Click To Hide/Show 3 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Gap junction alpha-8 protein (GJA8) Connexin family P48165
Disks large homolog 1 (DLG1) MAGUK family Q12959
Neuron-specific calcium-binding protein hippocalcin (HPCA) Recoverin family P84074
Other
Click To Hide/Show 5 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Protein BANP (BANP) BANP/SMAR1 family Q8N9N5
Protein FAM9B (FAM9B) FAM9 family Q8IZU0
Nuclear receptor 2C2-associated protein (NR2C2AP) NR2C2AP family Q86WQ0
Ubiquilin-1 (UBQLN1) . Q9UMX0
Ubiquilin-2 (UBQLN2) . Q9UHD9

The Drug(s) Related To This Target

Approved
Click To Hide/Show 4 Drug(s) Interacting with This Target
Drug Name Drug Type External ID
Gamma-hydroxybutyric Acid Small molecular drug DB01440
Lactic Acid Small molecular drug DB04398
Niacin Small molecular drug DB00627
Pyruvic Acid Small molecular drug DB00119
Investigative
Click To Hide/Show 8 Drug(s) Interacting with This Target
Drug Name Drug Type External ID
Alpha-ketoisovalerate Small molecular drug DB04074
D-lactic Acid Small molecular drug DB03066
Ferulic Acid Small molecular drug DB07767
Formic Acid Small molecular drug DB01942
Glyoxylic Acid Small molecular drug DB04343
Oxamic Acid Small molecular drug DB03940
Acetoacetic Acid . DB01762
Phenylpyruvic Acid . DB03884

References

1 Cyclopropenone, Cyclopropeniminium Ion, and Cyclopropenethione as Novel Electrophilic Warheads for Potential Target Discovery of Triple-Negative Breast Cancer. J Med Chem. 2023 Feb 23;66(4):2851-2864. doi: 10.1021/acs.jmedchem.2c01889. Epub 2023 Feb 10.
2 Ynamide Electrophile for the Profiling of Ligandable Carboxyl Residues in Live Cells and the Development of New Covalent Inhibitors. J Med Chem. 2022 Aug 11;65(15):10408-10418. doi: 10.1021/acs.jmedchem.2c00272. Epub 2022 Jul 26.
3 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
4 Oxidant-Induced Bioconjugation for Protein Labeling in Live Cells. ACS Chem Biol. 2023 Jan 20;18(1):112-122. doi: 10.1021/acschembio.2c00740. Epub 2022 Dec 21.
5 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
6 Global profiling of functional histidines in live cells using small-molecule photosensitizer and chemical probe relay labelling. Nat Chem. 2024 Jun 4. doi: 10.1038/s41557-024-01545-6. Online ahead of print.
Mass spectrometry data entry: PXD042377
7 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
8 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
9 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
10 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
11 Design and synthesis of minimalist terminal alkyne-containing diazirine photo-crosslinkers and their incorporation into kinase inhibitors for cell- and tissue-based proteome profiling. Angew Chem Int Ed Engl. 2013 Aug 12;52(33):8551-6. doi: 10.1002/anie.201300683. Epub 2013 Jun 10.
12 Pharmacological Targeting of Vacuolar H(+)-ATPase via Subunit V1G Combats Multidrug-Resistant Cancer. Cell Chem Biol. 2020 Nov 19;27(11):1359-1370.e8. doi: 10.1016/j.chembiol.2020.06.011. Epub 2020 Jul 9.
13 Proteome profiling reveals potential cellular targets of staurosporine using a clickable cell-permeable probe. Chem Commun (Camb). 2011 Oct 28;47(40):11306-8. doi: 10.1039/c1cc14824a. Epub 2011 Sep 16.