Details of the Target

General Information of Target

Target ID LDTP00300
Target Name Probable ubiquitin carboxyl-terminal hydrolase FAF-Y (USP9Y)
Gene Name USP9Y
Gene ID 8287
Synonyms
DFFRY; Probable ubiquitin carboxyl-terminal hydrolase FAF-Y; EC 3.4.19.12; Deubiquitinating enzyme FAF-Y; Fat facets protein-related, Y-linked; Ubiquitin thioesterase FAF-Y; Ubiquitin-specific protease 9, Y chromosome; Ubiquitin-specific-processing protease FAF-Y
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MTAITHGSPVGGNDSQGQVLDGQSQHLFQQNQTSSPDSSNENSVATPPPEEQGQGDAPPQ
HEDEEPAFPHTELANLDDMINRPRWVVPVLPKGELEVLLEAAIDLSVKGLDVKSEACQRF
FRDGLTISFTKILMDEAVSGWKFEIHRCIINNTHRLVELCVAKLSQDWFPLLELLAMALN
PHCKFHIYNGTRPCELISSNAQLPEDELFARSSDPRSPKGWLVDLINKFGTLNGFQILHD
RFFNGSALNIQIIAALIKPFGQCYEFLSQHTLKKYFIPVIEIVPHLLENLTDEELKKEAK
NEAKNDALSMIIKSLKNLASRISGQDETIKNLEIFRLKMILRLLQISSFNGKMNALNEIN
KVISSVSYYTHRHSNPEEEEWLTAERMAEWIQQNNILSIVLQDSLHQPQYVEKLEKILRF
VIKEKALTLQDLDNIWAAQAGKHEAIVKNVHDLLAKLAWDFSPGQLDHLFDCFKASWTNA
SKKQREKLLELIRRLAEDDKDGVMAHKVLNLLWNLAQSDDVPVDIMDLALSAHIKILDYS
CSQDRDAQKIQWIDHFIEELRTNDKWVIPALKQIREICSLFGEASQNLSQTQRSPHIFYR
HDLINQLQQNHALVTLVAENLATYMNSIRLYAGDHEDYDPQTVRLGSRYSHVQEVQERLN
FLRFLLKDGQLWLCAPQAKQIWKCLAENAVYLCDREACFKWYSKLMGDEPDLDPDINKDF
FESNVLQLDPSLLTENGMKCFERFFKAVNCRERKLIAKRRSYMMDDLELIGLDYLWRVVI
QSSDEIANRAIDLLKEIYTNLGPRLKANQVVIHEDFIQSCFDRLKASYDTLCVFDGDKNS
INCARQEAIRMVRVLTVIKEYINECDSDYHKERMILPMSRAFRGKHLSLIVRFPNQGRQV
DELDIWSHTNDTIGSVRRCIVNRIKANVAHKKIELFVGGELIDSEDDRKLIGQLNLKDKS
LITAKLTQINFNMPSSPDSSSDSSTASPGNHRNHYNDGPNLEVESCLPGVIMSVHPRYIS
FLWQVADLGSNLNMPPLRDGARVLMKLMPPDRTAVEKLRAVCLDHAKLGEGKLSPPLDSL
FFGPSASQVLYLTEVVYALLMPAGVPLTDGSSDFQVHFLKSGGLPLVLSMLIRNNFLPNT
DMETRRGAYLNALKIAKLLLTAIGYGHVRAVAEACQPVVDGTDPITQINQVTHDQAVVLQ
SALQSIPNPSSECVLRNESILLAQEISNEASRYMPDICVIRAIQKIIWASACGALGLVFS
PNEEITKIYQMTTNGSNKLEVEDEQVCCEALEVMTLCFALLPTALDALSKEKAWQTFIID
LLLHCPSKTVRQLAQEQFFLMCTRCCMGHRPLLFFITLLFTILGSTAREKGKYSGDYFTL
LRHLLNYAYNGNINIPNAEVLLVSEIDWLKRIRDNVKNTGETGVEEPILEGHLGVTKELL
AFQTSEKKYHFGCEKGGANLIKELIDDFIFPASKVYLQYLRSGELPAEQAIPVCSSPVTI
NAGFELLVALAIGCVRNLKQIVDCLTEMYYMGTAITTCEALTEWEYLPPVGPRPPKGFVG
LKNAGATCYMNSVIQQLYMIPSIRNSILAIEGTGSDLHDDMFGDEKQDSESNVDPRDDVF
GYPHQFEDKPALSKTEDRKEYNIGVLRHLQVIFGHLAASQLQYYVPRGFWKQFRLWGEPV
NLREQHDALEFFNSLVDSLDEALKALGHPAILSKVLGGSFADQKICQGCPHRYECEESFT
TLNVDIRNHQNLLDSLEQYIKGDLLEGANAYHCEKCDKKVDTVKRLLIKKLPRVLAIQLK
RFDYDWERECAIKFNDYFEFPRELDMGPYTVAGVANLERDNVNSENELIEQKEQSDNETA
GGTKYRLVGVLVHSGQASGGHYYSYIIQRNGKDDQTDHWYKFDDGDVTECKMDDDEEMKN
QCFGGEYMGEVFDHMMKRMSYRRQKRWWNAYILFYEQMDMIDEDDEMIRYISELTIARPH
QIIMSPAIERSVRKQNVKFMHNRLQYSLEYFQFVKKLLTCNGVYLNPAPGQDYLLPEAEE
ITMISIQLAARFLFTTGFHTKKIVRGPASDWYDALCVLLRHSKNVRFWFTHNVLFNVSNR
FSEYLLECPSAEVRGAFAKLIVFIAHFSLQDGSCPSPFASPGPSSQACDNLSLSDHLLRA
TLNLLRREVSEHGHHLQQYFNLFVMYANLGVAEKTQLLKLNVPATFMLVSLDEGPGPPIK
YQYAELGKLYSVVSQLIRCCNVSSTMQSSINGNPPLPNPFGDLNLSQPIMPIQQNVLDIL
FVRTSYVKKIIEDCSNSEDTIKLLRFCSWENPQFSSTVLSELLWQVAYSYTYELRPYLDL
LFQILLIEDSWQTHRIHNALKGIPDDRDGLFDTIQRSKNHYQKRAYQCIKCMVALFSSCP
VAYQILQGNGDLKRKWTWAVEWLGDELERRPYTGNPQYSYNNWSPPVQSNETANGYFLER
SHSARMTLAKACELCPEEEPDDQDAPDEHEPSPSEDAPLYPHSPASQYQQNNHVHGQPYT
GPAAHHLNNPQKTGQRTQENYEGNEEVSSPQMKDQ
Target Bioclass
Enzyme
Family
Peptidase C19 family
Subcellular location
Cytoplasm
Function
May function as a ubiquitin-protein or polyubiquitin hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. May therefore play an important regulatory role at the level of protein turnover by preventing degradation of proteins through the removal of conjugated ubiquitin. Essential component of TGF-beta/BMP signaling cascade. Deubiquitinates monoubiquitinated SMAD4, opposing the activity of E3 ubiquitin-protein ligase TRIM33. Monoubiquitination of SMAD4 hampers its ability to form a stable complex with activated SMAD2/3 resulting in inhibition of TGF-beta/BMP signaling cascade. Deubiquitination of SMAD4 by USP9X re-empowers its competence to mediate TGF-beta signaling.
Uniprot ID
O00507
Ensemble ID
ENST00000338981.7
HGNC ID
HGNC:12633

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
HCT116 SNV: p.N13S; p.M764I .
KPNYN SNV: p.Y1097F DBIA    Probe Info 
LNCaP clone FGC SNV: p.C865Y DBIA    Probe Info 
MEWO SNV: p.G2424R DBIA    Probe Info 
RL SNV: p.W459Ter DBIA    Probe Info 
SNU1 SNV: p.Q2005Ter DBIA    Probe Info 

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 14 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
Probe 1
 Probe Info  		Y649(2.17)  LDD3495  [1]
DBIA
 Probe Info  		C1062(5.21); C148(2.88); C674(2.45); C1773(1.03)  LDD3311  [2]
BTD
 Probe Info  		C117(0.65)  LDD2148  [3]
AHL-Pu-1
 Probe Info  		C541(2.12); C674(3.51); C1238(2.24)  LDD0168  [4]
IA-alkyne
 Probe Info  		C148(2.24); C674(2.11)  LDD0371  [4]
HHS-465
 Probe Info  		Y368(2.99)  LDD2237  [5]
W1
 Probe Info  		C1238(0.58)  LDD0239  [6]
IPM
 Probe Info  		N.A.  LDD0147  [7]
NPM
 Probe Info  		N.A.  LDD0016  [8]
PF-06672131
 Probe Info  		N.A.  LDD0017  [9]
Acrolein
 Probe Info  		H635(0.00); C2108(0.00); H651(0.00); H443(0.00)  LDD0217  [10]
Methacrolein
 Probe Info  		C1910(0.00); C160(0.00)  LDD0218  [10]
m-APA
 Probe Info  		N.A.  LDD0404  [11]
NAIA_5
 Probe Info  		N.A.  LDD2223  [12]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0025  4SU-RNA HEK-293T C541(2.12); C674(3.51); C1238(2.24)  LDD0168  [4]
 LDCM0026  4SU-RNA+native RNA HEK-293T C674(3.33)  LDD0169  [4]
 LDCM0214  AC1 HEK-293T C148(1.02); C1773(0.85); C1238(1.21); C674(1.09)  LDD1507  [13]
 LDCM0215  AC10 HEK-293T C148(0.98); C1773(1.02); C1238(0.95); C674(1.06)  LDD1508  [13]
 LDCM0226  AC11 HEK-293T C148(1.00); C1773(0.95); C1238(0.97); C674(0.85)  LDD1509  [13]
 LDCM0237  AC12 HEK-293T C148(0.97); C1773(1.04); C1238(0.97); C674(0.97)  LDD1510  [13]
 LDCM0259  AC14 HEK-293T C148(1.01); C1773(0.96); C1238(0.94); C674(0.89)  LDD1512  [13]
 LDCM0270  AC15 HEK-293T C148(0.96); C1773(1.08); C1238(1.02); C674(0.89)  LDD1513  [13]
 LDCM0276  AC17 HEK-293T C148(1.05); C1773(0.91); C1238(0.99); C674(0.91)  LDD1515  [13]
 LDCM0277  AC18 HEK-293T C148(0.98); C1773(1.00); C1238(0.99); C674(1.25)  LDD1516  [13]
 LDCM0278  AC19 HEK-293T C148(1.10); C1773(0.73); C1238(1.00); C674(0.94)  LDD1517  [13]
 LDCM0279  AC2 HEK-293T C148(1.04); C1773(1.02); C1238(0.96); C674(1.03)  LDD1518  [13]
 LDCM0280  AC20 HEK-293T C148(1.00); C1773(0.92); C1238(0.92); C674(0.93)  LDD1519  [13]
 LDCM0281  AC21 HEK-293T C148(1.01); C1773(0.94); C1238(0.98); C674(0.93)  LDD1520  [13]
 LDCM0282  AC22 HEK-293T C148(1.03); C1773(0.95); C1238(0.98); C674(0.94)  LDD1521  [13]
 LDCM0283  AC23 HEK-293T C148(0.93); C1773(1.09); C1238(0.93); C674(0.82)  LDD1522  [13]
 LDCM0284  AC24 HEK-293T C148(0.96); C1773(1.00); C1238(0.91); C674(0.86)  LDD1523  [13]
 LDCM0285  AC25 HEK-293T C148(1.04); C1773(0.92); C1238(1.08); C674(0.94)  LDD1524  [13]
 LDCM0286  AC26 HEK-293T C148(1.02); C1773(0.99); C1238(0.93); C674(0.98)  LDD1525  [13]
 LDCM0287  AC27 HEK-293T C148(1.00); C1773(0.94); C1238(1.00); C674(0.85)  LDD1526  [13]
 LDCM0288  AC28 HEK-293T C148(0.96); C1773(1.02); C1238(0.96); C674(0.96)  LDD1527  [13]
 LDCM0289  AC29 HEK-293T C148(0.99); C1773(0.86); C1238(0.95); C674(0.92)  LDD1528  [13]
 LDCM0290  AC3 HEK-293T C148(0.93); C1773(0.91); C1238(0.99); C674(1.07)  LDD1529  [13]
 LDCM0291  AC30 HEK-293T C148(0.96); C1773(0.98); C1238(1.05); C674(0.91)  LDD1530  [13]
 LDCM0292  AC31 HEK-293T C148(0.93); C1773(1.05); C1238(0.99); C674(0.91)  LDD1531  [13]
 LDCM0293  AC32 HEK-293T C148(0.97); C1773(0.96); C1238(0.91); C674(0.93)  LDD1532  [13]
 LDCM0294  AC33 HEK-293T C148(1.02); C1773(0.85); C1238(1.04); C674(0.90)  LDD1533  [13]
 LDCM0295  AC34 HEK-293T C148(0.96); C1773(0.94); C1238(0.95); C674(1.06)  LDD1534  [13]
 LDCM0296  AC35 HEK-293T C148(0.99); C1773(0.91); C1238(0.96); C674(0.84)  LDD1535  [13]
 LDCM0297  AC36 HEK-293T C148(1.03); C1773(1.08); C1238(0.99); C674(1.00)  LDD1536  [13]
 LDCM0298  AC37 HEK-293T C148(0.95); C1773(0.99); C1238(0.95); C674(0.93)  LDD1537  [13]
 LDCM0299  AC38 HEK-293T C148(1.06); C1773(0.98); C1238(0.97); C674(0.89)  LDD1538  [13]
 LDCM0300  AC39 HEK-293T C148(0.95); C1773(1.01); C1238(0.97); C674(0.86)  LDD1539  [13]
 LDCM0301  AC4 HEK-293T C148(0.99); C1773(1.07); C1238(0.91); C674(1.09)  LDD1540  [13]
 LDCM0302  AC40 HEK-293T C148(0.88); C1773(0.90); C1238(0.97); C674(0.83)  LDD1541  [13]
 LDCM0303  AC41 HEK-293T C148(0.95); C1773(0.87); C1238(1.18); C674(0.93)  LDD1542  [13]
 LDCM0304  AC42 HEK-293T C148(0.91); C1773(0.94); C1238(1.03); C674(1.06)  LDD1543  [13]
 LDCM0305  AC43 HEK-293T C148(0.92); C1773(0.96); C1238(0.99); C674(0.94)  LDD1544  [13]
 LDCM0306  AC44 HEK-293T C148(0.96); C1773(1.09); C1238(0.93); C674(0.96)  LDD1545  [13]
 LDCM0307  AC45 HEK-293T C148(0.95); C1773(0.92); C1238(0.98); C674(1.01)  LDD1546  [13]
 LDCM0308  AC46 HEK-293T C148(1.01); C1773(0.92); C1238(0.98); C674(0.88)  LDD1547  [13]
 LDCM0309  AC47 HEK-293T C148(0.88); C1773(1.07); C1238(0.98); C674(0.99)  LDD1548  [13]
 LDCM0310  AC48 HEK-293T C148(0.89); C1773(0.96); C1238(1.02); C674(0.87)  LDD1549  [13]
 LDCM0311  AC49 HEK-293T C148(0.97); C1773(0.89); C1238(1.07); C674(0.96)  LDD1550  [13]
 LDCM0312  AC5 HEK-293T C148(0.99); C1773(0.94); C1238(0.97); C674(0.99)  LDD1551  [13]
 LDCM0313  AC50 HEK-293T C148(1.04); C1773(1.01); C1238(0.91); C674(1.01)  LDD1552  [13]
 LDCM0314  AC51 HEK-293T C148(0.99); C1773(0.94); C1238(0.99); C674(0.91)  LDD1553  [13]
 LDCM0315  AC52 HEK-293T C148(0.98); C1773(1.08); C1238(0.99); C674(0.92)  LDD1554  [13]
 LDCM0316  AC53 HEK-293T C148(0.97); C1773(0.96); C1238(0.98); C674(0.95)  LDD1555  [13]
 LDCM0317  AC54 HEK-293T C148(1.00); C1773(0.95); C1238(0.95); C674(0.90)  LDD1556  [13]
 LDCM0318  AC55 HEK-293T C148(0.93); C1773(1.04); C1238(0.99); C674(0.90)  LDD1557  [13]
 LDCM0319  AC56 HEK-293T C148(0.98); C1773(0.90); C1238(1.03); C674(0.91)  LDD1558  [13]
 LDCM0320  AC57 HEK-293T C148(0.99); C1773(0.81); C1238(1.07); C674(0.98)  LDD1559  [13]
 LDCM0321  AC58 HEK-293T C148(0.99); C1773(0.97); C1238(0.96); C674(1.01)  LDD1560  [13]
 LDCM0322  AC59 HEK-293T C148(0.98); C1773(0.93); C1238(0.97); C674(0.96)  LDD1561  [13]
 LDCM0323  AC6 HEK-293T C148(1.01); C1773(0.90); C1238(1.00); C674(0.97)  LDD1562  [13]
 LDCM0324  AC60 HEK-293T C148(0.99); C1773(1.05); C1238(0.97); C674(1.00)  LDD1563  [13]
 LDCM0325  AC61 HEK-293T C148(1.00); C1773(0.91); C1238(1.01); C674(1.02)  LDD1564  [13]
 LDCM0326  AC62 HEK-293T C148(1.02); C1773(0.95); C1238(1.05); C674(0.96)  LDD1565  [13]
 LDCM0327  AC63 HEK-293T C148(0.92); C1773(1.01); C1238(0.97); C674(0.94)  LDD1566  [13]
 LDCM0328  AC64 HEK-293T C148(1.01); C1773(0.94); C1238(0.95); C674(1.07)  LDD1567  [13]
 LDCM0334  AC7 HEK-293T C148(0.91); C1773(1.08); C1238(0.98); C674(0.92)  LDD1568  [13]
 LDCM0345  AC8 HEK-293T C148(1.01); C1773(1.00); C1238(0.96); C674(0.92)  LDD1569  [13]
 LDCM0248  AKOS034007472 HEK-293T C148(1.04); C1773(0.96); C1238(0.97); C674(0.89)  LDD1511  [13]
 LDCM0356  AKOS034007680 HEK-293T C148(1.01); C1773(0.83); C1238(0.96); C674(0.96)  LDD1570  [13]
 LDCM0275  AKOS034007705 HEK-293T C148(0.94); C1773(0.97); C1238(0.99); C674(0.87)  LDD1514  [13]
 LDCM0156  Aniline NCI-H1299 N.A.  LDD0404  [11]
 LDCM0108  Chloroacetamide HeLa C160(0.00); H651(0.00); C2108(0.00); H635(0.00)  LDD0222  [10]
 LDCM0367  CL1 HEK-293T C148(0.91); C1773(1.04); C1238(0.92); C674(0.95)  LDD1571  [13]
 LDCM0368  CL10 HEK-293T C148(1.33); C1773(0.91); C1238(1.38); C674(1.87)  LDD1572  [13]
 LDCM0369  CL100 HEK-293T C148(1.03); C1773(0.90); C1238(1.00); C674(1.03)  LDD1573  [13]
 LDCM0370  CL101 HEK-293T C148(0.96); C1773(0.97); C1238(0.93); C674(1.01)  LDD1574  [13]
 LDCM0371  CL102 HEK-293T C148(1.10); C1773(0.82); C1238(1.04); C674(0.93)  LDD1575  [13]
 LDCM0372  CL103 HEK-293T C148(0.88); C1773(0.94); C1238(0.98); C674(0.85)  LDD1576  [13]
 LDCM0373  CL104 HEK-293T C148(0.97); C1773(0.95); C1238(0.96); C674(0.99)  LDD1577  [13]
 LDCM0374  CL105 HEK-293T C148(0.96); C1773(0.88); C1238(0.99); C674(1.03)  LDD1578  [13]
 LDCM0375  CL106 HEK-293T C148(1.14); C1773(0.83); C1238(0.99); C674(0.89)  LDD1579  [13]
 LDCM0376  CL107 HEK-293T C148(0.93); C1773(0.95); C1238(0.96); C674(0.80)  LDD1580  [13]
 LDCM0377  CL108 HEK-293T C148(1.02); C1773(0.92); C1238(0.98); C674(0.93)  LDD1581  [13]
 LDCM0378  CL109 HEK-293T C148(1.01); C1773(0.97); C1238(0.93); C674(1.08)  LDD1582  [13]
 LDCM0379  CL11 HEK-293T C148(1.00); C1773(1.14); C1238(1.25); C674(0.63)  LDD1583  [13]
 LDCM0380  CL110 HEK-293T C148(1.15); C1773(0.82); C1238(1.07); C674(0.96)  LDD1584  [13]
 LDCM0381  CL111 HEK-293T C148(0.92); C1773(0.93); C1238(1.02); C674(0.85)  LDD1585  [13]
 LDCM0382  CL112 HEK-293T C148(0.93); C1773(0.89); C1238(0.96); C674(0.97)  LDD1586  [13]
 LDCM0383  CL113 HEK-293T C148(0.99); C1773(0.95); C1238(0.92); C674(0.98)  LDD1587  [13]
 LDCM0384  CL114 HEK-293T C148(0.97); C1773(0.79); C1238(1.07); C674(0.96)  LDD1588  [13]
 LDCM0385  CL115 HEK-293T C148(0.92); C1773(0.92); C1238(1.03); C674(0.84)  LDD1589  [13]
 LDCM0386  CL116 HEK-293T C148(0.97); C1773(0.96); C1238(1.02); C674(0.92)  LDD1590  [13]
 LDCM0387  CL117 HEK-293T C148(0.94); C1773(0.99); C1238(0.95); C674(0.96)  LDD1591  [13]
 LDCM0388  CL118 HEK-293T C148(1.01); C1773(0.85); C1238(1.01); C674(0.93)  LDD1592  [13]
 LDCM0389  CL119 HEK-293T C148(0.99); C1773(0.94); C1238(0.99); C674(0.86)  LDD1593  [13]
 LDCM0390  CL12 HEK-293T C148(1.12); C1773(1.00); C1238(1.09); C674(0.59)  LDD1594  [13]
 LDCM0391  CL120 HEK-293T C148(1.05); C1773(0.96); C1238(0.89); C674(0.97)  LDD1595  [13]
 LDCM0392  CL121 HEK-293T C148(1.03); C1773(0.98); C1238(0.91); C674(0.95)  LDD1596  [13]
 LDCM0393  CL122 HEK-293T C148(1.04); C1773(0.84); C1238(1.05); C674(1.01)  LDD1597  [13]
 LDCM0394  CL123 HEK-293T C148(0.94); C1773(0.90); C1238(1.01); C674(0.85)  LDD1598  [13]
 LDCM0395  CL124 HEK-293T C148(1.10); C1773(0.90); C1238(0.95); C674(0.97)  LDD1599  [13]
 LDCM0396  CL125 HEK-293T C148(0.98); C1773(0.92); C1238(0.91); C674(1.30)  LDD1600  [13]
 LDCM0397  CL126 HEK-293T C148(1.17); C1773(0.85); C1238(1.07); C674(1.07)  LDD1601  [13]
 LDCM0398  CL127 HEK-293T C148(0.98); C1773(0.94); C1238(1.00); C674(1.07)  LDD1602  [13]
 LDCM0399  CL128 HEK-293T C148(0.99); C1773(0.94); C1238(0.99); C674(1.22)  LDD1603  [13]
 LDCM0400  CL13 HEK-293T C148(0.90); C1773(0.95); C1238(0.96); C674(0.97)  LDD1604  [13]
 LDCM0401  CL14 HEK-293T C148(1.11); C1773(0.93); C1238(1.00); C674(0.88)  LDD1605  [13]
 LDCM0402  CL15 HEK-293T C148(1.06); C1773(0.92); C1238(1.04); C674(0.66)  LDD1606  [13]
 LDCM0403  CL16 HEK-293T C148(0.94); C1773(1.09); C1238(1.00); C674(0.86)  LDD1607  [13]
 LDCM0404  CL17 HEK-293T C148(1.22); C1773(0.82); C1238(1.07); C674(0.79)  LDD1608  [13]
 LDCM0405  CL18 HEK-293T C148(1.00); C1773(1.01); C1238(0.96); C674(0.85)  LDD1609  [13]
 LDCM0406  CL19 HEK-293T C148(1.02); C1773(0.97); C1238(0.94); C674(0.80)  LDD1610  [13]
 LDCM0407  CL2 HEK-293T C148(1.03); C1773(0.93); C1238(1.08); C674(0.89)  LDD1611  [13]
 LDCM0408  CL20 HEK-293T C148(1.04); C1773(1.10); C1238(1.01); C674(0.68)  LDD1612  [13]
 LDCM0409  CL21 HEK-293T C148(1.13); C1773(0.89); C1238(1.07); C674(0.65)  LDD1613  [13]
 LDCM0410  CL22 HEK-293T C148(1.13); C1773(0.99); C1238(1.05); C674(0.66)  LDD1614  [13]
 LDCM0411  CL23 HEK-293T C148(1.06); C1773(1.15); C1238(1.11); C674(0.65)  LDD1615  [13]
 LDCM0412  CL24 HEK-293T C148(1.03); C1773(0.99); C1238(1.11); C674(0.64)  LDD1616  [13]
 LDCM0413  CL25 HEK-293T C148(1.04); C1773(0.90); C1238(0.93); C674(1.19)  LDD1617  [13]
 LDCM0414  CL26 HEK-293T C148(0.93); C1773(0.91); C1238(0.99); C674(0.89)  LDD1618  [13]
 LDCM0415  CL27 HEK-293T C148(0.97); C1773(1.03); C1238(1.02); C674(0.77)  LDD1619  [13]
 LDCM0416  CL28 HEK-293T C148(1.03); C1773(0.92); C1238(1.00); C674(0.83)  LDD1620  [13]
 LDCM0417  CL29 HEK-293T C148(1.01); C1773(0.88); C1238(0.96); C674(0.75)  LDD1621  [13]
 LDCM0418  CL3 HEK-293T C148(0.96); C1773(1.00); C1238(1.09); C674(0.72)  LDD1622  [13]
 LDCM0419  CL30 HEK-293T C148(1.01); C1773(0.96); C1238(0.91); C674(0.82)  LDD1623  [13]
 LDCM0420  CL31 HEK-293T C148(1.06); C1773(0.90); C1238(1.06); C674(0.74)  LDD1624  [13]
 LDCM0421  CL32 HEK-293T C148(1.08); C1773(0.92); C1238(0.99); C674(0.75)  LDD1625  [13]
 LDCM0422  CL33 HEK-293T C148(1.02); C1773(0.83); C1238(1.08); C674(1.08)  LDD1626  [13]
 LDCM0423  CL34 HEK-293T C148(1.21); C1773(0.99); C1238(1.04); C674(0.63)  LDD1627  [13]
 LDCM0424  CL35 HEK-293T C148(1.03); C1773(0.93); C1238(1.13); C674(0.62)  LDD1628  [13]
 LDCM0425  CL36 HEK-293T C148(1.11); C1773(1.01); C1238(1.04); C674(0.60)  LDD1629  [13]
 LDCM0426  CL37 HEK-293T C148(1.03); C1773(1.02); C1238(0.90); C674(1.10)  LDD1630  [13]
 LDCM0428  CL39 HEK-293T C148(0.89); C1773(0.92); C1238(0.94); C674(0.80)  LDD1632  [13]
 LDCM0429  CL4 HEK-293T C148(0.94); C1773(1.01); C1238(1.03); C674(0.87)  LDD1633  [13]
 LDCM0430  CL40 HEK-293T C148(0.98); C1773(0.98); C1238(1.02); C674(0.82)  LDD1634  [13]
 LDCM0431  CL41 HEK-293T C148(1.03); C1773(0.85); C1238(1.02); C674(0.75)  LDD1635  [13]
 LDCM0432  CL42 HEK-293T C148(1.11); C1773(0.99); C1238(1.00); C674(0.79)  LDD1636  [13]
 LDCM0433  CL43 HEK-293T C148(1.04); C1773(0.97); C1238(0.95); C674(0.67)  LDD1637  [13]
 LDCM0434  CL44 HEK-293T C148(1.05); C1773(1.08); C1238(1.02); C674(0.80)  LDD1638  [13]
 LDCM0435  CL45 HEK-293T C148(1.03); C1773(0.38); C1238(1.11); C674(2.78)  LDD1639  [13]
 LDCM0436  CL46 HEK-293T C148(1.17); C1773(0.97); C1238(1.04); C674(0.70)  LDD1640  [13]
 LDCM0437  CL47 HEK-293T C148(1.05); C1773(1.01); C1238(1.19); C674(0.58)  LDD1641  [13]
 LDCM0438  CL48 HEK-293T C148(1.09); C1773(0.90); C1238(0.99); C674(0.61)  LDD1642  [13]
 LDCM0439  CL49 HEK-293T C148(0.96); C1773(0.97); C1238(0.96); C674(1.06)  LDD1643  [13]
 LDCM0440  CL5 HEK-293T C148(0.93); C1773(0.91); C1238(1.18); C674(0.88)  LDD1644  [13]
 LDCM0441  CL50 HEK-293T C148(1.07); C1773(0.79); C1238(1.15); C674(0.91)  LDD1645  [13]
 LDCM0443  CL52 HEK-293T C148(1.03); C1773(0.90); C1238(0.95); C674(0.84)  LDD1646  [13]
 LDCM0444  CL53 HEK-293T C148(1.19); C1773(0.76); C1238(0.99); C674(0.76)  LDD1647  [13]
 LDCM0445  CL54 HEK-293T C148(1.01); C1773(0.85); C1238(0.97); C674(0.83)  LDD1648  [13]
 LDCM0446  CL55 HEK-293T C148(1.09); C1773(0.94); C1238(1.34); C674(0.70)  LDD1649  [13]
 LDCM0447  CL56 HEK-293T C148(1.06); C1773(1.09); C1238(0.97); C674(0.69)  LDD1650  [13]
 LDCM0448  CL57 HEK-293T C148(1.02); C1773(0.85); C1238(1.13); C674(0.63)  LDD1651  [13]
 LDCM0449  CL58 HEK-293T C148(1.14); C1773(0.93); C1238(1.05); C674(0.60)  LDD1652  [13]
 LDCM0450  CL59 HEK-293T C148(1.01); C1773(1.09); C1238(1.07); C674(0.77)  LDD1653  [13]
 LDCM0451  CL6 HEK-293T C148(1.07); C1773(0.97); C1238(0.94); C674(0.83)  LDD1654  [13]
 LDCM0452  CL60 HEK-293T C148(1.14); C1773(0.94); C1238(1.05); C674(0.63)  LDD1655  [13]
 LDCM0453  CL61 HEK-293T C148(1.05); C1773(1.01); C1238(0.90); C674(1.15)  LDD1656  [13]
 LDCM0454  CL62 HEK-293T C148(1.10); C1773(0.89); C1238(1.05); C674(1.03)  LDD1657  [13]
 LDCM0455  CL63 HEK-293T C148(0.97); C1773(0.91); C1238(1.11); C674(0.77)  LDD1658  [13]
 LDCM0456  CL64 HEK-293T C148(1.12); C1773(0.92); C1238(0.99); C674(0.82)  LDD1659  [13]
 LDCM0457  CL65 HEK-293T C148(0.93); C1773(0.86); C1238(1.04); C674(0.83)  LDD1660  [13]
 LDCM0458  CL66 HEK-293T C148(0.98); C1773(0.95); C1238(0.96); C674(0.81)  LDD1661  [13]
 LDCM0459  CL67 HEK-293T C148(1.09); C1773(0.95); C1238(1.03); C674(0.74)  LDD1662  [13]
 LDCM0460  CL68 HEK-293T C148(1.07); C1773(1.06); C1238(1.01); C674(0.77)  LDD1663  [13]
 LDCM0461  CL69 HEK-293T C148(0.98); C1773(0.97); C1238(1.05); C674(0.69)  LDD1664  [13]
 LDCM0462  CL7 HEK-293T C148(1.12); C1773(0.98); C1238(0.97); C674(0.75)  LDD1665  [13]
 LDCM0463  CL70 HEK-293T C148(1.24); C1773(0.96); C1238(1.06); C674(0.67)  LDD1666  [13]
 LDCM0464  CL71 HEK-293T C148(0.99); C1773(1.13); C1238(1.15); C674(0.64)  LDD1667  [13]
 LDCM0465  CL72 HEK-293T C148(1.09); C1773(1.01); C1238(1.05); C674(0.60)  LDD1668  [13]
 LDCM0466  CL73 HEK-293T C148(1.03); C1773(0.97); C1238(0.92); C674(1.00)  LDD1669  [13]
 LDCM0467  CL74 HEK-293T C148(1.02); C1773(0.91); C1238(1.00); C674(1.00)  LDD1670  [13]
 LDCM0469  CL76 HEK-293T C148(1.00); C1773(0.94); C1238(1.04); C674(0.87)  LDD1672  [13]
 LDCM0470  CL77 HEK-293T C148(1.04); C1773(0.79); C1238(0.94); C674(0.79)  LDD1673  [13]
 LDCM0471  CL78 HEK-293T C148(1.04); C1773(0.91); C1238(0.91); C674(0.87)  LDD1674  [13]
 LDCM0472  CL79 HEK-293T C148(1.11); C1773(1.04); C1238(0.97); C674(0.76)  LDD1675  [13]
 LDCM0473  CL8 HEK-293T C148(1.56); C1773(0.90); C1238(0.96); C674(0.75)  LDD1676  [13]
 LDCM0474  CL80 HEK-293T C148(1.10); C1773(1.19); C1238(1.02); C674(0.75)  LDD1677  [13]
 LDCM0475  CL81 HEK-293T C148(1.09); C1773(1.01); C1238(1.00); C674(0.70)  LDD1678  [13]
 LDCM0476  CL82 HEK-293T C148(1.12); C1773(0.94); C1238(1.00); C674(0.72)  LDD1679  [13]
 LDCM0477  CL83 HEK-293T C148(0.99); C1773(0.99); C1238(1.06); C674(0.65)  LDD1680  [13]
 LDCM0478  CL84 HEK-293T C148(1.06); C1773(0.90); C1238(1.02); C674(0.66)  LDD1681  [13]
 LDCM0479  CL85 HEK-293T C148(0.99); C1773(0.96); C1238(0.89); C674(1.33)  LDD1682  [13]
 LDCM0480  CL86 HEK-293T C148(0.95); C1773(0.80); C1238(1.01); C674(1.19)  LDD1683  [13]
 LDCM0481  CL87 HEK-293T C148(0.98); C1773(0.93); C1238(1.06); C674(0.96)  LDD1684  [13]
 LDCM0482  CL88 HEK-293T C148(0.98); C1773(0.99); C1238(1.04); C674(1.04)  LDD1685  [13]
 LDCM0483  CL89 HEK-293T C148(1.04); C1773(0.86); C1238(0.99); C674(0.89)  LDD1686  [13]
 LDCM0484  CL9 HEK-293T C148(1.13); C1773(0.98); C1238(0.97); C674(2.82)  LDD1687  [13]
 LDCM0485  CL90 HEK-293T C148(0.90); C1773(0.80); C1238(1.00); C674(1.13)  LDD1688  [13]
 LDCM0486  CL91 HEK-293T C148(1.05); C1773(0.95); C1238(0.96); C674(0.76)  LDD1689  [13]
 LDCM0487  CL92 HEK-293T C148(0.95); C1773(1.03); C1238(0.95); C674(0.88)  LDD1690  [13]
 LDCM0488  CL93 HEK-293T C148(0.94); C1773(0.87); C1238(1.03); C674(0.70)  LDD1691  [13]
 LDCM0489  CL94 HEK-293T C148(1.11); C1773(0.85); C1238(1.17); C674(0.83)  LDD1692  [13]
 LDCM0490  CL95 HEK-293T C148(1.02); C1773(0.83); C1238(1.12); C674(0.71)  LDD1693  [13]
 LDCM0491  CL96 HEK-293T C148(1.05); C1773(0.85); C1238(1.03); C674(0.75)  LDD1694  [13]
 LDCM0492  CL97 HEK-293T C148(1.07); C1773(0.97); C1238(0.98); C674(1.22)  LDD1695  [13]
 LDCM0493  CL98 HEK-293T C148(1.04); C1773(0.80); C1238(1.09); C674(1.33)  LDD1696  [13]
 LDCM0494  CL99 HEK-293T C148(0.91); C1773(0.99); C1238(1.04); C674(2.24)  LDD1697  [13]
 LDCM0495  E2913 HEK-293T C148(0.94); C1773(0.86); C1238(1.01); C674(0.75)  LDD1698  [13]
 LDCM0203  EV-96 T cell C843(10.49)  LDD0523  [14]
 LDCM0468  Fragment33 HEK-293T C148(0.85); C1773(0.92); C1238(1.00); C674(0.84)  LDD1671  [13]
 LDCM0427  Fragment51 HEK-293T C148(0.90); C1773(0.88); C1238(1.03); C674(0.98)  LDD1631  [13]
 LDCM0107  IAA HeLa H651(0.00); H451(0.00); C160(0.00); C2108(0.00)  LDD0221  [10]
 LDCM0022  KB02 22RV1 C148(2.56); C1773(1.08)  LDD2243  [2]
 LDCM0023  KB03 22RV1 C148(3.89); C1773(1.53)  LDD2660  [2]
 LDCM0024  KB05 G361 C1062(5.21); C148(2.88); C674(2.45); C1773(1.03)  LDD3311  [2]
 LDCM0109  NEM HeLa H651(0.00); H635(0.00); H451(0.00)  LDD0223  [10]
 LDCM0554  Nucleophilic fragment 7a MDA-MB-231 C117(0.65)  LDD2148  [3]
 LDCM0627  NUDT7-COV-1 HEK-293T C148(1.33); C674(0.77)  LDD2206  [15]
 LDCM0628  OTUB2-COV-1 HEK-293T C117(0.76); C148(0.67)  LDD2207  [15]
 LDCM0112  W16 Hep-G2 C1238(0.58)  LDD0239  [6]

References

1 An Azo Coupling-Based Chemoproteomic Approach to Systematically Profile the Tyrosine Reactivity in the Human Proteome. Anal Chem. 2021 Jul 27;93(29):10334-10342. doi: 10.1021/acs.analchem.1c01935. Epub 2021 Jul 12.
2 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
3 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
4 Chemoproteomic capture of RNA binding activity in living cells. Nat Commun. 2023 Oct 7;14(1):6282. doi: 10.1038/s41467-023-41844-z.
Mass spectrometry data entry: PXD044625
5 Global targeting of functional tyrosines using sulfur-triazole exchange chemistry. Nat Chem Biol. 2020 Feb;16(2):150-159. doi: 10.1038/s41589-019-0404-5. Epub 2019 Nov 25.
6 Oxidant-Induced Bioconjugation for Protein Labeling in Live Cells. ACS Chem Biol. 2023 Jan 20;18(1):112-122. doi: 10.1021/acschembio.2c00740. Epub 2022 Dec 21.
7 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
8 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
9 Site-Specific Activity-Based Protein Profiling Using Phosphonate Handles. Mol Cell Proteomics. 2023 Jan;22(1):100455. doi: 10.1016/j.mcpro.2022.100455. Epub 2022 Nov 24.
Mass spectrometry data entry: PXD036569
10 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
11 Quantitative and Site-Specific Chemoproteomic Profiling of Targets of Acrolein. Chem Res Toxicol. 2019 Mar 18;32(3):467-473. doi: 10.1021/acs.chemrestox.8b00343. Epub 2019 Jan 15.
12 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
13 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
14 An Activity-Guided Map of Electrophile-Cysteine Interactions in Primary Human T Cells. Cell. 2020 Aug 20;182(4):1009-1026.e29. doi: 10.1016/j.cell.2020.07.001. Epub 2020 Jul 29.
15 Rapid Covalent-Probe Discovery by Electrophile-Fragment Screening. J Am Chem Soc. 2019 Jun 5;141(22):8951-8968. doi: 10.1021/jacs.9b02822. Epub 2019 May 22.