Details of the Target

General Information of Target

Target ID LDTP00160
Target Name Espin (ESPN)
Gene Name ESPN
Gene ID 83715
Synonyms
DFNB36; Espin; Autosomal recessive deafness type 36 protein; Ectoplasmic specialization protein
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MALEQALQAARQGELDVLRSLHAAGLLGPSLRDPLDALPVHHAARAGKLHCLRFLVEEAA
LPAAARARNGATPAHDASATGHLACLQWLLSQGGCRVQDKDNSGATVLHLAARFGHPEVV
NWLLHHGGGDPTAATDMGALPIHYAAAKGDFPSLRLLVEHYPEGVNAQTKNGATPLYLAC
QEGHLEVTQYLVQECGADPHARAHDGMTPLHAAAQMGHSPVIVWLVSCTDVSLSEQDKDG
ATAMHFAASRGHTKVLSWLLLHGGEISADLWGGTPLHDAAENGELECCQILVVNGAELDV
RDRDGYTAADLSDFNGHSHCTRYLRTVENLSVEHRVLSRDPSAELEAKQPDSGMSSPNTT
VSVQPLNFDLSSPTSTLSNYDSCSSSHSSIKGQHPPCGLSSARAADIQSYMDMLNPELGL
PRGTIGKPTPPPPPPSFPPPPPPPGTQLPPPPPGYPAPKPPVGPQAADIYMQTKNKLRHV
ETEALKKELSSCDGHDGLRRQDSSRKPRAFSKQPSTGDYYRQLGRCPGETLAARPGMAHS
EEVRARQPARAGCPRLGPAARGSLEGPSAPPQAALLPGNHVPNGCAADPKASRELPPPPP
PPPPPLPEAASSPPPAPPLPLESAGPGCGQRRSSSSTGSTKSFNMMSPTGDNSELLAEIK
AGKSLKPTPQSKGLTTVFSGIGQPAFQPDSPLPSVSPALSPVRSPTPPAAGFQPLLNGSL
VPVPPTTPAPGVQLDVEALIPTHDEQGRPIPEWKRQVMVRKMQLKMQEEEEQRRKEEEEE
ARLASMPAWRRDLLRKKLEEEREQKRKEEERQKQEELRREKEQSEKLRTLGYDESKLAPW
QRQVILKKGDIAKY
Target Bioclass
Other
Subcellular location
Cytoplasm, cytoskeleton
Function
Multifunctional actin-bundling protein. Plays a major role in regulating the organization, dimension, dynamics and signaling capacities of the actin filament-rich microvilli in the mechanosensory and chemosensory cells. Required for the assembly and stabilization of the stereociliary parallel actin bundles. Plays a crucial role in the formation and maintenance of inner ear hair cell stereocilia. Involved in the elongation of actin in stereocilia. In extrastriolar hair cells, required for targeting MYO3B to stereocilia tips, and for regulation of stereocilia diameter and staircase formation.
Uniprot ID
B1AK53
Ensemble ID
ENST00000461727.6
HGNC ID
HGNC:13281

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
22RV1 Insertion: p.T649DfsTer10 .
CCFSTTG1 SNV: p.S338F .
ETK1 SNV: p.A280S .
HCC1937 SNV: p.P444S .
HT115 SNV: p.V581A .
JHH1 SNV: p.K148Ter .
KPL1 SNV: p.P452T .
MCC13 Substitution: p.G392R .
MCC26 SNV: p.C287G .
MCF7 SNV: p.P452T .
MDST8 SNV: p.A405V .
MEWO SNV: p.P433L; p.P648S .
MFE319 SNV: p.A616V .
NCIH2172 SNV: p.A212D .
OCUG1 SNV: p.E57K .
SKMM2 SNV: p.G316A .
TC71 SNV: p.R499L .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 3 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C526(0.89)  LDD0531  [1]
NAIA_5
 Probe Info  		N.A.  LDD2225  [2]
TFBX
 Probe Info  		N.A.  LDD0148  [3]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0214  AC1 HCT 116 C526(0.89)  LDD0531  [1]
 LDCM0215  AC10 HCT 116 C526(1.17)  LDD0532  [1]
 LDCM0226  AC11 HCT 116 C526(0.99)  LDD0543  [1]
 LDCM0230  AC113 HCT 116 C526(1.13)  LDD0547  [1]
 LDCM0231  AC114 HCT 116 C526(0.77)  LDD0548  [1]
 LDCM0232  AC115 HCT 116 C526(0.69)  LDD0549  [1]
 LDCM0233  AC116 HCT 116 C526(1.00)  LDD0550  [1]
 LDCM0234  AC117 HCT 116 C526(0.97)  LDD0551  [1]
 LDCM0235  AC118 HCT 116 C526(0.85)  LDD0552  [1]
 LDCM0236  AC119 HCT 116 C526(1.07)  LDD0553  [1]
 LDCM0237  AC12 HCT 116 C526(1.18)  LDD0554  [1]
 LDCM0238  AC120 HCT 116 C526(0.96)  LDD0555  [1]
 LDCM0239  AC121 HCT 116 C526(0.71)  LDD0556  [1]
 LDCM0240  AC122 HCT 116 C526(0.95)  LDD0557  [1]
 LDCM0241  AC123 HCT 116 C526(0.92)  LDD0558  [1]
 LDCM0242  AC124 HCT 116 C526(0.65)  LDD0559  [1]
 LDCM0243  AC125 HCT 116 C526(0.73)  LDD0560  [1]
 LDCM0244  AC126 HCT 116 C526(0.76)  LDD0561  [1]
 LDCM0245  AC127 HCT 116 C526(0.98)  LDD0562  [1]
 LDCM0246  AC128 HCT 116 C526(0.88)  LDD0563  [1]
 LDCM0247  AC129 HCT 116 C526(0.67)  LDD0564  [1]
 LDCM0249  AC130 HCT 116 C526(0.88)  LDD0566  [1]
 LDCM0250  AC131 HCT 116 C526(0.88)  LDD0567  [1]
 LDCM0251  AC132 HCT 116 C526(1.07)  LDD0568  [1]
 LDCM0252  AC133 HCT 116 C526(1.18)  LDD0569  [1]
 LDCM0253  AC134 HCT 116 C526(1.25)  LDD0570  [1]
 LDCM0254  AC135 HCT 116 C526(1.13)  LDD0571  [1]
 LDCM0255  AC136 HCT 116 C526(1.00)  LDD0572  [1]
 LDCM0256  AC137 HCT 116 C526(0.88)  LDD0573  [1]
 LDCM0257  AC138 HCT 116 C526(0.99)  LDD0574  [1]
 LDCM0258  AC139 HCT 116 C526(1.16)  LDD0575  [1]
 LDCM0259  AC14 HCT 116 C526(1.13)  LDD0576  [1]
 LDCM0260  AC140 HCT 116 C526(1.23)  LDD0577  [1]
 LDCM0261  AC141 HCT 116 C526(1.23)  LDD0578  [1]
 LDCM0262  AC142 HCT 116 C526(0.83)  LDD0579  [1]
 LDCM0263  AC143 HCT 116 C526(1.41)  LDD0580  [1]
 LDCM0264  AC144 HCT 116 C526(1.12)  LDD0581  [1]
 LDCM0265  AC145 HCT 116 C526(1.08)  LDD0582  [1]
 LDCM0266  AC146 HCT 116 C526(1.53)  LDD0583  [1]
 LDCM0267  AC147 HCT 116 C526(0.97)  LDD0584  [1]
 LDCM0268  AC148 HCT 116 C526(2.66)  LDD0585  [1]
 LDCM0269  AC149 HCT 116 C526(1.68)  LDD0586  [1]
 LDCM0270  AC15 HCT 116 C526(1.12)  LDD0587  [1]
 LDCM0271  AC150 HCT 116 C526(1.18)  LDD0588  [1]
 LDCM0272  AC151 HCT 116 C526(0.93)  LDD0589  [1]
 LDCM0273  AC152 HCT 116 C526(1.04)  LDD0590  [1]
 LDCM0274  AC153 HCT 116 C526(1.94)  LDD0591  [1]
 LDCM0621  AC154 HCT 116 C526(1.11)  LDD2158  [1]
 LDCM0622  AC155 HCT 116 C526(1.33)  LDD2159  [1]
 LDCM0623  AC156 HCT 116 C526(1.36)  LDD2160  [1]
 LDCM0624  AC157 HCT 116 C526(1.10)  LDD2161  [1]
 LDCM0276  AC17 HCT 116 C526(0.82)  LDD0593  [1]
 LDCM0277  AC18 HCT 116 C526(0.79)  LDD0594  [1]
 LDCM0278  AC19 HCT 116 C526(0.92)  LDD0595  [1]
 LDCM0279  AC2 HCT 116 C526(0.99)  LDD0596  [1]
 LDCM0280  AC20 HCT 116 C526(0.96)  LDD0597  [1]
 LDCM0281  AC21 HCT 116 C526(0.77)  LDD0598  [1]
 LDCM0282  AC22 HCT 116 C526(0.98)  LDD0599  [1]
 LDCM0283  AC23 HCT 116 C526(0.85)  LDD0600  [1]
 LDCM0284  AC24 HCT 116 C526(0.80)  LDD0601  [1]
 LDCM0285  AC25 HCT 116 C526(1.25)  LDD0602  [1]
 LDCM0286  AC26 HCT 116 C526(1.40)  LDD0603  [1]
 LDCM0287  AC27 HCT 116 C526(2.01)  LDD0604  [1]
 LDCM0288  AC28 HCT 116 C526(2.09)  LDD0605  [1]
 LDCM0289  AC29 HCT 116 C526(1.75)  LDD0606  [1]
 LDCM0290  AC3 HCT 116 C526(0.90)  LDD0607  [1]
 LDCM0291  AC30 HCT 116 C526(2.21)  LDD0608  [1]
 LDCM0292  AC31 HCT 116 C526(1.49)  LDD0609  [1]
 LDCM0293  AC32 HCT 116 C526(1.92)  LDD0610  [1]
 LDCM0294  AC33 HCT 116 C526(1.27)  LDD0611  [1]
 LDCM0295  AC34 HCT 116 C526(1.19)  LDD0612  [1]
 LDCM0296  AC35 HCT 116 C526(0.90)  LDD0613  [1]
 LDCM0297  AC36 HCT 116 C526(1.05)  LDD0614  [1]
 LDCM0298  AC37 HCT 116 C526(0.81)  LDD0615  [1]
 LDCM0299  AC38 HCT 116 C526(1.23)  LDD0616  [1]
 LDCM0300  AC39 HCT 116 C526(1.01)  LDD0617  [1]
 LDCM0301  AC4 HCT 116 C526(0.77)  LDD0618  [1]
 LDCM0302  AC40 HCT 116 C526(1.01)  LDD0619  [1]
 LDCM0303  AC41 HCT 116 C526(1.41)  LDD0620  [1]
 LDCM0304  AC42 HCT 116 C526(1.07)  LDD0621  [1]
 LDCM0305  AC43 HCT 116 C526(1.90)  LDD0622  [1]
 LDCM0306  AC44 HCT 116 C526(0.90)  LDD0623  [1]
 LDCM0307  AC45 HCT 116 C526(1.02)  LDD0624  [1]
 LDCM0308  AC46 HCT 116 C526(1.02)  LDD0625  [1]
 LDCM0309  AC47 HCT 116 C526(1.05)  LDD0626  [1]
 LDCM0310  AC48 HCT 116 C526(1.10)  LDD0627  [1]
 LDCM0311  AC49 HCT 116 C526(1.38)  LDD0628  [1]
 LDCM0312  AC5 HCT 116 C526(0.89)  LDD0629  [1]
 LDCM0313  AC50 HCT 116 C526(1.29)  LDD0630  [1]
 LDCM0314  AC51 HCT 116 C526(1.25)  LDD0631  [1]
 LDCM0315  AC52 HCT 116 C526(1.16)  LDD0632  [1]
 LDCM0316  AC53 HCT 116 C526(1.16)  LDD0633  [1]
 LDCM0317  AC54 HCT 116 C526(1.06)  LDD0634  [1]
 LDCM0318  AC55 HCT 116 C526(1.03)  LDD0635  [1]
 LDCM0319  AC56 HCT 116 C526(1.14)  LDD0636  [1]
 LDCM0323  AC6 HCT 116 C526(1.23)  LDD0640  [1]
 LDCM0334  AC7 HCT 116 C526(1.00)  LDD0651  [1]
 LDCM0345  AC8 HCT 116 C526(1.02)  LDD0662  [1]
 LDCM0349  AC83 HCT 116 C526(1.16)  LDD0666  [1]
 LDCM0350  AC84 HCT 116 C526(1.26)  LDD0667  [1]
 LDCM0351  AC85 HCT 116 C526(1.37)  LDD0668  [1]
 LDCM0352  AC86 HCT 116 C526(1.19)  LDD0669  [1]
 LDCM0353  AC87 HCT 116 C526(1.53)  LDD0670  [1]
 LDCM0354  AC88 HCT 116 C526(1.17)  LDD0671  [1]
 LDCM0355  AC89 HCT 116 C526(1.30)  LDD0672  [1]
 LDCM0357  AC90 HCT 116 C526(1.38)  LDD0674  [1]
 LDCM0358  AC91 HCT 116 C526(1.46)  LDD0675  [1]
 LDCM0359  AC92 HCT 116 C526(1.47)  LDD0676  [1]
 LDCM0360  AC93 HCT 116 C526(1.29)  LDD0677  [1]
 LDCM0361  AC94 HCT 116 C526(1.30)  LDD0678  [1]
 LDCM0362  AC95 HCT 116 C526(1.41)  LDD0679  [1]
 LDCM0363  AC96 HCT 116 C526(1.12)  LDD0680  [1]
 LDCM0364  AC97 HCT 116 C526(1.57)  LDD0681  [1]
 LDCM0248  AKOS034007472 HCT 116 C526(1.08)  LDD0565  [1]
 LDCM0356  AKOS034007680 HCT 116 C526(1.18)  LDD0673  [1]
 LDCM0275  AKOS034007705 HCT 116 C526(1.22)  LDD0592  [1]
 LDCM0020  ARS-1620 HCC44 C526(1.06)  LDD2171  [1]
 LDCM0632  CL-Sc Hep-G2 C492(20.00)  LDD2227  [2]
 LDCM0367  CL1 HCT 116 C526(1.11)  LDD0684  [1]
 LDCM0368  CL10 HCT 116 C526(0.97)  LDD0685  [1]
 LDCM0369  CL100 HCT 116 C526(0.93)  LDD0686  [1]
 LDCM0370  CL101 HCT 116 C526(1.27)  LDD0687  [1]
 LDCM0371  CL102 HCT 116 C526(1.62)  LDD0688  [1]
 LDCM0372  CL103 HCT 116 C526(1.11)  LDD0689  [1]
 LDCM0373  CL104 HCT 116 C526(1.17)  LDD0690  [1]
 LDCM0374  CL105 HCT 116 C526(0.78)  LDD0691  [1]
 LDCM0375  CL106 HCT 116 C526(0.74)  LDD0692  [1]
 LDCM0376  CL107 HCT 116 C526(0.74)  LDD0693  [1]
 LDCM0377  CL108 HCT 116 C526(0.79)  LDD0694  [1]
 LDCM0378  CL109 HCT 116 C526(0.85)  LDD0695  [1]
 LDCM0379  CL11 HCT 116 C526(1.56)  LDD0696  [1]
 LDCM0380  CL110 HCT 116 C526(0.96)  LDD0697  [1]
 LDCM0381  CL111 HCT 116 C526(1.10)  LDD0698  [1]
 LDCM0382  CL112 HCT 116 C526(1.31)  LDD0699  [1]
 LDCM0383  CL113 HCT 116 C526(1.42)  LDD0700  [1]
 LDCM0384  CL114 HCT 116 C526(1.86)  LDD0701  [1]
 LDCM0385  CL115 HCT 116 C526(1.86)  LDD0702  [1]
 LDCM0386  CL116 HCT 116 C526(1.39)  LDD0703  [1]
 LDCM0387  CL117 HCT 116 C526(1.08)  LDD0704  [1]
 LDCM0388  CL118 HCT 116 C526(1.25)  LDD0705  [1]
 LDCM0389  CL119 HCT 116 C526(1.42)  LDD0706  [1]
 LDCM0390  CL12 HCT 116 C526(1.05)  LDD0707  [1]
 LDCM0391  CL120 HCT 116 C526(1.55)  LDD0708  [1]
 LDCM0392  CL121 HCT 116 C526(0.86)  LDD0709  [1]
 LDCM0393  CL122 HCT 116 C526(0.94)  LDD0710  [1]
 LDCM0394  CL123 HCT 116 C526(1.53)  LDD0711  [1]
 LDCM0395  CL124 HCT 116 C526(1.19)  LDD0712  [1]
 LDCM0400  CL13 HCT 116 C526(0.82)  LDD0717  [1]
 LDCM0401  CL14 HCT 116 C526(1.22)  LDD0718  [1]
 LDCM0402  CL15 HCT 116 C526(1.08)  LDD0719  [1]
 LDCM0403  CL16 HCT 116 C526(1.03)  LDD0720  [1]
 LDCM0404  CL17 HCT 116 C526(1.73)  LDD0721  [1]
 LDCM0405  CL18 HCT 116 C526(1.11)  LDD0722  [1]
 LDCM0406  CL19 HCT 116 C526(0.93)  LDD0723  [1]
 LDCM0407  CL2 HCT 116 C526(1.17)  LDD0724  [1]
 LDCM0408  CL20 HCT 116 C526(1.22)  LDD0725  [1]
 LDCM0409  CL21 HCT 116 C526(1.51)  LDD0726  [1]
 LDCM0410  CL22 HCT 116 C526(0.92)  LDD0727  [1]
 LDCM0411  CL23 HCT 116 C526(0.97)  LDD0728  [1]
 LDCM0412  CL24 HCT 116 C526(1.04)  LDD0729  [1]
 LDCM0413  CL25 HCT 116 C526(1.13)  LDD0730  [1]
 LDCM0414  CL26 HCT 116 C526(1.17)  LDD0731  [1]
 LDCM0415  CL27 HCT 116 C526(1.01)  LDD0732  [1]
 LDCM0416  CL28 HCT 116 C526(1.27)  LDD0733  [1]
 LDCM0417  CL29 HCT 116 C526(1.30)  LDD0734  [1]
 LDCM0418  CL3 HCT 116 C526(1.01)  LDD0735  [1]
 LDCM0419  CL30 HCT 116 C526(1.22)  LDD0736  [1]
 LDCM0420  CL31 HCT 116 C526(1.14)  LDD0737  [1]
 LDCM0421  CL32 HCT 116 C526(1.02)  LDD0738  [1]
 LDCM0422  CL33 HCT 116 C526(1.38)  LDD0739  [1]
 LDCM0423  CL34 HCT 116 C526(1.08)  LDD0740  [1]
 LDCM0424  CL35 HCT 116 C526(1.17)  LDD0741  [1]
 LDCM0425  CL36 HCT 116 C526(1.52)  LDD0742  [1]
 LDCM0426  CL37 HCT 116 C526(1.07)  LDD0743  [1]
 LDCM0428  CL39 HCT 116 C526(1.14)  LDD0745  [1]
 LDCM0429  CL4 HCT 116 C526(0.95)  LDD0746  [1]
 LDCM0430  CL40 HCT 116 C526(2.73)  LDD0747  [1]
 LDCM0431  CL41 HCT 116 C526(1.31)  LDD0748  [1]
 LDCM0432  CL42 HCT 116 C526(1.28)  LDD0749  [1]
 LDCM0433  CL43 HCT 116 C526(1.34)  LDD0750  [1]
 LDCM0434  CL44 HCT 116 C526(1.41)  LDD0751  [1]
 LDCM0435  CL45 HCT 116 C526(1.48)  LDD0752  [1]
 LDCM0440  CL5 HCT 116 C526(0.81)  LDD0757  [1]
 LDCM0451  CL6 HCT 116 C526(1.05)  LDD0768  [1]
 LDCM0462  CL7 HCT 116 C526(0.83)  LDD0779  [1]
 LDCM0473  CL8 HCT 116 C526(2.05)  LDD0790  [1]
 LDCM0484  CL9 HCT 116 C526(0.95)  LDD0801  [1]
 LDCM0486  CL91 HCT 116 C526(1.21)  LDD0803  [1]
 LDCM0487  CL92 HCT 116 C526(1.17)  LDD0804  [1]
 LDCM0488  CL93 HCT 116 C526(1.11)  LDD0805  [1]
 LDCM0489  CL94 HCT 116 C526(1.21)  LDD0806  [1]
 LDCM0490  CL95 HCT 116 C526(1.25)  LDD0807  [1]
 LDCM0491  CL96 HCT 116 C526(0.99)  LDD0808  [1]
 LDCM0492  CL97 HCT 116 C526(1.15)  LDD0809  [1]
 LDCM0493  CL98 HCT 116 C526(0.94)  LDD0810  [1]
 LDCM0494  CL99 HCT 116 C526(0.94)  LDD0811  [1]
 LDCM0427  Fragment51 HCT 116 C526(6.02)  LDD0744  [1]
 LDCM0022  KB02 AU565 C397(2.75)  LDD2265  [4]
 LDCM0023  KB03 AU565 C397(2.64)  LDD2682  [4]
 LDCM0024  KB05 SNU-308 C397(4.41)  LDD3441  [4]
 LDCM0021  THZ1 HCT 116 C526(1.06)  LDD2173  [1]

References

1 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
2 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
3 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
4 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840